LOCUS       DAZBWR010000001        78564 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000001 DAZBWR010000000
VERSION     DAZBWR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.001"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..726)
                     /locus_tag="ACHGT5_000001"
     CDS             complement(61..726)
                     /locus_tag="ACHGT5_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN7216025.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACHGT5_000002"
     CDS             complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACHGT5_000002"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN7216026.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACHGT5_000003"
     CDS             complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACHGT5_000003"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN7216027.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACHGT5_000004"
     CDS             complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACHGT5_000004"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN7216028.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3040..3360
                     /locus_tag="ACHGT5_000005"
     CDS             3040..3360
                     /locus_tag="ACHGT5_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN7216029.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3472..4329)
                     /locus_tag="ACHGT5_000006"
     CDS             complement(3472..4329)
                     /locus_tag="ACHGT5_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN7216030.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4437..5741)
                     /locus_tag="ACHGT5_000007"
     CDS             complement(4437..5741)
                     /locus_tag="ACHGT5_000007"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN7216031.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5738..6247)
                     /locus_tag="ACHGT5_000008"
     CDS             complement(5738..6247)
                     /locus_tag="ACHGT5_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216032.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6422..8557)
                     /locus_tag="ACHGT5_000009"
     CDS             complement(6422..8557)
                     /locus_tag="ACHGT5_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN7216033.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8640..10295)
                     /locus_tag="ACHGT5_000010"
     CDS             complement(8640..10295)
                     /locus_tag="ACHGT5_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN7216034.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10297..10611)
                     /locus_tag="ACHGT5_000011"
     CDS             complement(10297..10611)
                     /locus_tag="ACHGT5_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN7216035.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10781..11041
                     /locus_tag="ACHGT5_000012"
     CDS             10781..11041
                     /locus_tag="ACHGT5_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN7216036.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11055..11369
                     /locus_tag="ACHGT5_000013"
     CDS             11055..11369
                     /locus_tag="ACHGT5_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN7216037.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12099..12653)
                     /locus_tag="ACHGT5_000014"
     CDS             complement(12099..12653)
                     /locus_tag="ACHGT5_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN7216038.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12779..14708)
                     /locus_tag="ACHGT5_000015"
                     /pseudo
     CDS             complement(12779..14708)
                     /locus_tag="ACHGT5_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15014..15730)
                     /locus_tag="ACHGT5_000016"
     CDS             complement(15014..15730)
                     /locus_tag="ACHGT5_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN7216039.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACHGT5_000017"
     CDS             complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACHGT5_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN7216040.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACHGT5_000018"
     CDS             complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACHGT5_000018"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN7216041.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19200..20660)
                     /locus_tag="ACHGT5_000019"
     CDS             complement(19200..20660)
                     /locus_tag="ACHGT5_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216042.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20650..21945)
                     /locus_tag="ACHGT5_000020"
     CDS             complement(20650..21945)
                     /locus_tag="ACHGT5_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN7216043.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22277..22954)
                     /locus_tag="ACHGT5_000021"
     CDS             complement(22277..22954)
                     /locus_tag="ACHGT5_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN7216044.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22984..24075)
                     /locus_tag="ACHGT5_000022"
     CDS             complement(22984..24075)
                     /locus_tag="ACHGT5_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN7216045.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24176..24610)
                     /locus_tag="ACHGT5_000023"
     CDS             complement(24176..24610)
                     /locus_tag="ACHGT5_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN7216046.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25194..26651)
                     /locus_tag="ACHGT5_000024"
     CDS             complement(25194..26651)
                     /locus_tag="ACHGT5_000024"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN7216047.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26648..28543)
                     /locus_tag="ACHGT5_000025"
     CDS             complement(26648..28543)
                     /locus_tag="ACHGT5_000025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN7216048.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28601..30376)
                     /locus_tag="ACHGT5_000026"
     CDS             complement(28601..30376)
                     /locus_tag="ACHGT5_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN7216049.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30426..31922)
                     /locus_tag="ACHGT5_000027"
     CDS             complement(30426..31922)
                     /locus_tag="ACHGT5_000027"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN7216050.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32116..33738)
                     /locus_tag="ACHGT5_000028"
     CDS             complement(32116..33738)
                     /locus_tag="ACHGT5_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN7216051.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34066..35379)
                     /locus_tag="ACHGT5_000029"
     CDS             complement(34066..35379)
                     /locus_tag="ACHGT5_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN7216052.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35624..35848)
                     /locus_tag="ACHGT5_000030"
     CDS             complement(35624..35848)
                     /locus_tag="ACHGT5_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216053.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35868..36290)
                     /locus_tag="ACHGT5_000031"
     CDS             complement(35868..36290)
                     /locus_tag="ACHGT5_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7216054.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(36432..37142)
                     /locus_tag="ACHGT5_000032"
     CDS             complement(36432..37142)
                     /locus_tag="ACHGT5_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN7216055.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37160..37657)
                     /locus_tag="ACHGT5_000033"
     CDS             complement(37160..37657)
                     /locus_tag="ACHGT5_000033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216056.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37694..38146)
                     /locus_tag="ACHGT5_000034"
     CDS             complement(37694..38146)
                     /locus_tag="ACHGT5_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN7216057.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(38281..38886)
                     /locus_tag="ACHGT5_000035"
     CDS             complement(38281..38886)
                     /locus_tag="ACHGT5_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN7216058.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(39336..39956)
                     /locus_tag="ACHGT5_000036"
     CDS             complement(39336..39956)
                     /locus_tag="ACHGT5_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7216059.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39958..40827)
                     /locus_tag="ACHGT5_000037"
     CDS             complement(39958..40827)
                     /locus_tag="ACHGT5_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN7216060.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40850..41257)
                     /locus_tag="ACHGT5_000038"
     CDS             complement(40850..41257)
                     /locus_tag="ACHGT5_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN7216061.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(41276..42565)
                     /locus_tag="ACHGT5_000039"
     CDS             complement(41276..42565)
                     /locus_tag="ACHGT5_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN7216062.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(42592..42894)
                     /locus_tag="ACHGT5_000040"
     CDS             complement(42592..42894)
                     /locus_tag="ACHGT5_000040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7216063.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42919..45012)
                     /locus_tag="ACHGT5_000041"
     CDS             complement(42919..45012)
                     /locus_tag="ACHGT5_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN7216064.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(45501..46265)
                     /locus_tag="ACHGT5_000042"
     CDS             complement(45501..46265)
                     /locus_tag="ACHGT5_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN7216065.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACHGT5_000043"
     CDS             complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACHGT5_000043"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN7216066.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(47270..48346)
                     /locus_tag="ACHGT5_000044"
     CDS             complement(47270..48346)
                     /locus_tag="ACHGT5_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN7216067.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(48370..48963)
                     /locus_tag="ACHGT5_000045"
     CDS             complement(48370..48963)
                     /locus_tag="ACHGT5_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN7216068.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACHGT5_000046"
     CDS             complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACHGT5_000046"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN7216069.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(50633..50932)
                     /locus_tag="ACHGT5_000047"
     CDS             complement(50633..50932)
                     /locus_tag="ACHGT5_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN7216070.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACHGT5_000048"
     CDS             complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACHGT5_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFN7216071.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(52687..53598)
                     /locus_tag="ACHGT5_000049"
     CDS             complement(52687..53598)
                     /locus_tag="ACHGT5_000049"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN7216072.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            53718..54575
                     /locus_tag="ACHGT5_000050"
     CDS             53718..54575
                     /locus_tag="ACHGT5_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN7216073.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(54762..55124)
                     /locus_tag="ACHGT5_000051"
     CDS             complement(54762..55124)
                     /locus_tag="ACHGT5_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN7216074.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(55313..55495)
                     /locus_tag="ACHGT5_000052"
     CDS             complement(55313..55495)
                     /locus_tag="ACHGT5_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216075.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            55470..56909
                     /locus_tag="ACHGT5_000053"
     CDS             55470..56909
                     /locus_tag="ACHGT5_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216076.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(56916..59468)
                     /locus_tag="ACHGT5_000054"
     CDS             complement(56916..59468)
                     /locus_tag="ACHGT5_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN7216077.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(59582..60913)
                     /locus_tag="ACHGT5_000055"
     CDS             complement(59582..60913)
                     /locus_tag="ACHGT5_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN7216078.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60879..62357)
                     /locus_tag="ACHGT5_000056"
     CDS             complement(60879..62357)
                     /locus_tag="ACHGT5_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN7216079.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(62662..64497)
                     /gene="tdc"
                     /locus_tag="ACHGT5_000057"
     CDS             complement(62662..64497)
                     /gene="tdc"
                     /locus_tag="ACHGT5_000057"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN7216080.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            64967..66394
                     /locus_tag="ACHGT5_000058"
     CDS             64967..66394
                     /locus_tag="ACHGT5_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216081.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(66486..66851)
                     /locus_tag="ACHGT5_000059"
     CDS             complement(66486..66851)
                     /locus_tag="ACHGT5_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN7216082.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(66848..67213)
                     /locus_tag="ACHGT5_000060"
     CDS             complement(66848..67213)
                     /locus_tag="ACHGT5_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN7216083.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(67405..67653)
                     /locus_tag="ACHGT5_000061"
     CDS             complement(67405..67653)
                     /locus_tag="ACHGT5_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216084.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            67652..67780
                     /locus_tag="ACHGT5_000062"
     CDS             67652..67780
                     /locus_tag="ACHGT5_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN7216085.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            67837..69531
                     /locus_tag="ACHGT5_000063"
     CDS             67837..69531
                     /locus_tag="ACHGT5_000063"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN7216086.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            69631..69927
                     /locus_tag="ACHGT5_000064"
     CDS             69631..69927
                     /locus_tag="ACHGT5_000064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216087.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            70305..71438
                     /locus_tag="ACHGT5_000065"
     CDS             70305..71438
                     /locus_tag="ACHGT5_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN7216088.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            71469..71789
                     /locus_tag="ACHGT5_000066"
     CDS             71469..71789
                     /locus_tag="ACHGT5_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN7216089.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(71818..72282)
                     /locus_tag="ACHGT5_000067"
     CDS             complement(71818..72282)
                     /locus_tag="ACHGT5_000067"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216090.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(72413..73666)
                     /locus_tag="ACHGT5_000068"
     CDS             complement(72413..73666)
                     /locus_tag="ACHGT5_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN7216091.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(73695..74012)
                     /locus_tag="ACHGT5_000069"
     CDS             complement(73695..74012)
                     /locus_tag="ACHGT5_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN7216092.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(74038..74358)
                     /locus_tag="ACHGT5_000070"
     CDS             complement(74038..74358)
                     /locus_tag="ACHGT5_000070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7216093.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(74822..76237)
                     /locus_tag="ACHGT5_000071"
     CDS             complement(74822..76237)
                     /locus_tag="ACHGT5_000071"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN7216094.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(76254..77570)
                     /locus_tag="ACHGT5_000072"
     CDS             complement(76254..77570)
                     /locus_tag="ACHGT5_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7216095.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(77720..78418)
                     /locus_tag="ACHGT5_000073"
     CDS             complement(77720..78418)
                     /locus_tag="ACHGT5_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN7216096.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT        22841 a        16682 c        13342 g        25699 t
ORIGIN      
        1 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
       61 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
      121 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
      181 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
      241 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
      301 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
      361 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
      421 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
      481 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
      541 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
      601 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
      661 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
      721 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
      781 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
      841 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
      901 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
      961 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     1021 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     1081 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     1141 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     1201 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     1261 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     1321 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     1381 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     1441 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     1501 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     1561 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     1621 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     1681 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     1741 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     1801 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     1861 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     1921 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     1981 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     2041 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     2101 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     2161 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     2221 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     2281 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     2341 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     2401 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     2461 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     2521 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     2581 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     2641 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     2701 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     2761 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     2821 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     2881 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     2941 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     3001 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     3061 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     3121 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     3181 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     3241 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     3301 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     3361 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     3421 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     3481 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     3541 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     3601 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     3661 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     3721 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     3781 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     3841 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     3901 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     3961 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     4021 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     4081 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     4141 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     4201 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     4261 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     4321 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     4381 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     4441 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     4501 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     4561 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     4621 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     4681 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     4741 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     4801 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     4861 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     4921 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     4981 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     5041 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     5101 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     5161 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     5221 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     5281 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     5341 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     5401 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     5461 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     5521 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     5581 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     5641 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     5701 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     5761 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     5821 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     5881 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     5941 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     6001 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     6061 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     6121 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     6181 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     6241 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     6301 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     6361 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     6421 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     6481 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
     6541 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
     6601 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
     6661 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
     6721 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
     6781 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
     6841 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
     6901 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
     6961 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
     7021 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
     7081 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
     7141 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
     7201 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
     7261 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
     7321 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
     7381 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
     7441 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
     7501 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
     7561 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
     7621 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
     7681 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
     7741 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
     7801 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
     7861 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
     7921 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
     7981 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
     8041 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
     8101 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
     8161 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
     8221 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
     8281 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
     8341 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
     8401 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
     8461 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
     8521 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
     8581 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
     8641 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
     8701 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
     8761 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
     8821 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
     8881 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
     8941 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
     9001 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
     9061 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
     9121 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
     9181 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
     9241 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
     9301 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
     9361 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
     9421 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
     9481 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
     9541 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
     9601 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
     9661 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
     9721 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
     9781 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
     9841 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
     9901 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
     9961 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    10021 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    10081 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    10141 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    10201 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    10261 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    10321 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    10381 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    10441 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    10501 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    10561 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    10621 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    10681 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    10741 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    10801 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    10861 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    10921 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    10981 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    11041 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    11101 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    11161 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    11221 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    11281 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    11341 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    11401 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    11461 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    11521 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    11581 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    11641 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    11701 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    11761 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    11821 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    11881 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    11941 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    12001 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    12061 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    12121 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    12181 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    12241 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    12301 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    12361 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    12421 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    12481 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    12541 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    12601 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    12661 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    12721 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    12781 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    12841 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    12901 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    12961 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    13021 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    13081 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    13141 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    13201 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    13261 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    13321 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    13381 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    13441 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    13501 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    13561 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    13621 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    13681 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    13741 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    13801 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    13861 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    13921 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    13981 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    14041 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    14101 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    14161 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    14221 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    14281 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    14341 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    14401 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    14461 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    14521 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    14581 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    14641 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    14701 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    14761 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    14821 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    14881 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    14941 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    15001 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    15061 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    15121 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    15181 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    15241 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    15301 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    15361 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    15421 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    15481 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    15541 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    15601 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    15661 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    15721 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    15781 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    15841 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    15901 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    15961 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    16021 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    16081 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    16141 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    16201 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    16261 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    16321 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    16381 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    16441 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    16501 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    16561 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    16621 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    16681 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    16741 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    16801 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    16861 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    16921 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    16981 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    17041 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    17101 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    17161 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    17221 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    17281 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    17341 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    17401 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    17461 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    17521 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    17581 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    17641 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    17701 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    17761 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    17821 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    17881 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    17941 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    18001 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    18061 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    18121 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    18181 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    18241 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    18301 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    18361 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    18421 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    18481 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    18541 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    18601 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    18661 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    18721 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    18781 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    18841 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    18901 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    18961 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    19021 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    19081 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    19141 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    19201 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    19261 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    19321 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    19381 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    19441 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    19501 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    19561 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    19621 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    19681 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    19741 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    19801 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    19861 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    19921 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    19981 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    20041 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    20101 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    20161 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    20221 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    20281 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    20341 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    20401 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    20461 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    20521 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    20581 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    20641 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    20701 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    20761 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    20821 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    20881 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    20941 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    21001 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    21061 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    21121 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    21181 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    21241 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    21301 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    21361 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    21421 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    21481 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    21541 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    21601 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    21661 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    21721 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    21781 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    21841 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    21901 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    21961 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    22021 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    22081 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    22141 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    22201 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    22261 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    22321 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    22381 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    22441 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    22501 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    22561 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    22621 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    22681 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    22741 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    22801 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    22861 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    22921 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    22981 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    23041 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    23101 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    23161 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    23221 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    23281 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    23341 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    23401 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    23461 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    23521 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    23581 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    23641 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    23701 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    23761 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    23821 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    23881 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    23941 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    24001 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    24061 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    24121 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    24181 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    24241 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    24301 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    24361 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    24421 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    24481 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    24541 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    24601 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    24661 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    24721 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    24781 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    24841 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    24901 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    24961 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    25021 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    25081 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    25141 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    25201 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    25261 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    25321 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    25381 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    25441 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    25501 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    25561 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    25621 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    25681 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    25741 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    25801 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    25861 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    25921 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    25981 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    26041 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    26101 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    26161 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    26221 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    26281 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    26341 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    26401 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    26461 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    26521 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    26581 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    26641 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    26701 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    26761 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    26821 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    26881 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    26941 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    27001 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    27061 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    27121 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    27181 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    27241 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    27301 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    27361 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    27421 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    27481 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    27541 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    27601 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    27661 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    27721 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    27781 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    27841 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    27901 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    27961 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    28021 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    28081 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    28141 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    28201 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    28261 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    28321 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    28381 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    28441 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    28501 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    28561 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    28621 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    28681 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    28741 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    28801 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    28861 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    28921 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    28981 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    29041 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    29101 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    29161 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    29221 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    29281 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    29341 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    29401 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    29461 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    29521 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    29581 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    29641 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    29701 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    29761 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    29821 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    29881 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    29941 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    30001 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    30061 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    30121 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    30181 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    30241 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    30301 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    30361 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    30421 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    30481 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    30541 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    30601 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    30661 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    30721 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    30781 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    30841 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    30901 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    30961 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    31021 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    31081 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    31141 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    31201 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    31261 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    31321 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    31381 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    31441 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    31501 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    31561 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    31621 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    31681 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    31741 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    31801 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    31861 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    31921 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    31981 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    32041 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    32101 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    32161 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    32221 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    32281 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    32341 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    32401 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    32461 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    32521 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    32581 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    32641 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    32701 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    32761 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    32821 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    32881 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    32941 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    33001 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    33061 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    33121 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    33181 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    33241 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    33301 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    33361 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    33421 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    33481 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    33541 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    33601 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    33661 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    33721 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    33781 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    33841 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    33901 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    33961 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    34021 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    34081 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    34141 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    34201 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    34261 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    34321 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    34381 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    34441 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    34501 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    34561 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    34621 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    34681 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    34741 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    34801 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    34861 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    34921 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    34981 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    35041 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    35101 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    35161 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    35221 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    35281 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    35341 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    35401 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    35461 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    35521 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    35581 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    35641 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    35701 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    35761 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    35821 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    35881 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    35941 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    36001 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    36061 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    36121 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    36181 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    36241 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    36301 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    36361 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    36421 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    36481 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    36541 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    36601 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    36661 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    36721 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    36781 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    36841 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    36901 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    36961 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    37021 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    37081 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    37141 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    37201 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    37261 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    37321 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    37381 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    37441 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    37501 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    37561 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    37621 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    37681 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    37741 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    37801 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    37861 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    37921 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    37981 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    38041 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    38101 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    38161 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    38221 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    38281 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    38341 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    38401 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    38461 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    38521 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    38581 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    38641 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    38701 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    38761 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    38821 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    38881 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    38941 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    39001 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    39061 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    39121 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    39181 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    39241 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    39301 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    39361 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    39421 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    39481 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    39541 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    39601 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    39661 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    39721 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    39781 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    39841 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    39901 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    39961 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    40021 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    40081 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    40141 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    40201 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    40261 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    40321 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    40381 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    40441 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    40501 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    40561 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    40621 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    40681 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    40741 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    40801 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    40861 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    40921 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    40981 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    41041 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    41101 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    41161 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    41221 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    41281 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    41341 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    41401 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    41461 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    41521 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    41581 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    41641 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    41701 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    41761 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    41821 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    41881 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    41941 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    42001 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    42061 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    42121 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    42181 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    42241 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    42301 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    42361 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    42421 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    42481 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    42541 cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta tttagataac
    42601 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
    42661 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
    42721 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
    42781 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
    42841 aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt tcatctttgt
    42901 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
    42961 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
    43021 ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa aaataatctt
    43081 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
    43141 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
    43201 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
    43261 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
    43321 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
    43381 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
    43441 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
    43501 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
    43561 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
    43621 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
    43681 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
    43741 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
    43801 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
    43861 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
    43921 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
    43981 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
    44041 taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
    44101 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
    44161 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
    44221 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
    44281 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
    44341 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
    44401 gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga ttttttgagc
    44461 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
    44521 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
    44581 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
    44641 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
    44701 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
    44761 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
    44821 caacaaaaaa cctttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
    44881 gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga actcacttgc
    44941 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
    45001 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
    45061 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
    45121 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
    45181 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    45241 aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat cgcagatgca
    45301 agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct tgagcgataa
    45361 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
    45421 ctatctctgg cacaccgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
    45481 ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc ggcttgccat
    45541 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
    45601 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
    45661 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
    45721 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
    45781 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
    45841 caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc tttcttttct
    45901 ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt ttgtctttta
    45961 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaat
    46021 cccgtttgct cataactaga aggacctgta cctggaccat aatacccata agaatctctg
    46081 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa tgaacctctg
    46141 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
    46201 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
    46261 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata gccttgctaa
    46321 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
    46381 cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct atcttcagca
    46441 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
    46501 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
    46561 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
    46621 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
    46681 ctagctcctt agctgcttga attgcaagcc tgatatgcgg ataccctaat atcgcttctt
    46741 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
    46801 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
    46861 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
    46921 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
    46981 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca tgatgccatg
    47041 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta agaaataaag
    47101 catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca ggttttggac
    47161 ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc tgagtcactg
    47221 ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat tacttgctga
    47281 aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca aatgcacaac
    47341 ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc agccaactat
    47401 ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac gtacgataag
    47461 cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca atcattttgg
    47521 aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg atcgtattca
    47581 agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa
    47641 agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc aaatattgat
    47701 agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta gcaacaaacc
    47761 gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt tgatagaaca
    47821 gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca tattttttat
    47881 tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg atctgcagca
    47941 tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc agtaaaaagc
    48001 ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa agataagccg
    48061 tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa aattcccaat
    48121 aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata aacattggtg
    48181 ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc
    48241 tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca gctgatactt
    48301 tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct ttctctctct
    48361 ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt tcaatagaat
    48421 ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc gagtcactac
    48481 ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa ttggcaattc
    48541 aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta ctgccggaat
    48601 cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc ctgataatat
    48661 gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct cttgtctata
    48721 cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat gttttgagat
    48781 ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc
    48841 gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa cgacccattc
    48901 aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat gtgcttgaat
    48961 cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg tttcgacacg
    49021 tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt
    49081 gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat tttgttctga
    49141 agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt ttgtataatt
    49201 attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt cagcaaccga
    49261 acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa tttctttatt
    49321 gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc
    49381 atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg cataagcagc
    49441 agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc cgtatgcttg
    49501 gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt tatcttcttc
    49561 gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt
    49621 tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga tggcttcttc
    49681 tatgaattta cttgatttac cataatacac agtactaata gaagaagttg gatttttcat
    49741 gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt catttccttc
    49801 tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt cattgtaatc
    49861 gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat aaaatgctga
    49921 gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag cagctttgat
    49981 tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc gttgacgaga
    50041 tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa cgcctgcttc
    50101 ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg cgattaaata
    50161 actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc ctggaatcaa
    50221 accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt ctgaaataga
    50281 taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta caatgactgg
    50341 aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag caacgatttt
    50401 tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag cgattactac
    50461 tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac tttcagggac
    50521 gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa tcaattcacg
    50581 tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa aactattttt
    50641 catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct ggtgtagcac
    50701 cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat ctcgttaata
    50761 cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca gtagattctg
    50821 ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact cctacatgga
    50881 ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc atggatctct
    50941 cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc ttccatctgg
    51001 gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga atttcattgg
    51061 tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg cagctaataa
    51121 tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca cgatgccggc
    51181 tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg taacaccgcc
    51241 aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca aataagcaat
    51301 tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg gtgcattggc
    51361 taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga ctggtttttt
    51421 atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc ctaatggatc
    51481 atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc caggattatg
    51541 tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg tagaagagtt
    51601 tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat attgtcctgc
    51661 agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc
    51721 tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat ggatgctttc
    51781 aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata cccagttctt
    51841 cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca attcgatagc
    51901 tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg cgtagattcc
    51961 tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag gatctcgtgt
    52021 gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta cccagtcacc
    52081 agggatatct acacgtggta aagaatcgac aacttcgtta acttgaacga tcacgatacc
    52141 atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga aacctgtgta
    52201 caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg gaatcaaatc
    52261 gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt tcattgtgcc
    52321 gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga aatcgatctt
    52381 gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac tagacatgat
    52441 caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg aaagaaaact
    52501 tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa tcagtttttc
    52561 cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt tgctgatttt
    52621 ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg gtttttccat
    52681 aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat gttggtctag
    52741 attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct ttgtcaaagt
    52801 attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca agtccatcat
    52861 ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa tctcttctgc
    52921 gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat cagatagatg
    52981 gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga gacagtgaga
    53041 tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa
    53101 cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac aattttcatt
    53161 tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct cagcttcgct
    53221 tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt ctttcattaa
    53281 gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc gacaagcaac
    53341 agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt
    53401 ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga taaaaaccgt
    53461 atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt ctaaacgttc
    53521 catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt gtccaggaaa
    53581 taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca ctgctatctt
    53641 agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat
    53701 ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa
    53761 caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc ttgagccgtg
    53821 cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc ggcagaaaca
    53881 ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca ttagacacat
    53941 tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat
    54001 tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt atggaaaagc
    54061 atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt accgatcctg
    54121 tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc atgaaagaac
    54181 ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat ctgcaattag
    54241 aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc cgtcatacac
    54301 tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg acagatgatc
    54361 ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc ccaaaagttt
    54421 cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat ttgcccttga
    54481 cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc aaaatgattg
    54541 caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg gacaaaattc
    54601 ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta gaaaataagc
    54661 cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    54721 aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt tgatttaaaa
    54781 cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag atcaatagag
    54841 atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc tcgcaaaatt
    54901 ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg gtcacttgca
    54961 tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt tttgcttttt
    55021 tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg agacaaattg
    55081 tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt ccatctcttt
    55141 cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa tacaagtagt
    55201 tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt caatccgcct
    55261 gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc acctattttt
    55321 tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca gtaaatgctc
    55381 cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt attttctccg
    55441 tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa atcattaccg
    55501 aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat acaaagataa
    55561 ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt tctgaccttc
    55621 aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa gcaggaatca
    55681 gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt ttcaaacaat
    55741 cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct gtccctgatt
    55801 ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact aaaatcaatc
    55861 agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct tgtattcttg
    55921 gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc actcataaca
    55981 actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt ttaagaaacc
    56041 tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt tggttcttta
    56101 tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat accaacagca
    56161 cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa ggtatttatc
    56221 ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg aattacaaca
    56281 ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta gaggtcctcg
    56341 aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa aaaacatctc
    56401 tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata ttgattgaag
    56461 cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat gcacacttgt
    56521 atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc aatgaaccaa
    56581 attactttct ccaactcttg tctctactga tccaacaaac gattttttct atcaaaccga
    56641 atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg ttcttgcagc
    56701 aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa ctaaccgatc
    56761 tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct ctagattctc
    56821 tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc aatcgtttga
    56881 ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc tctagctgtt
    56941 ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac aactaagaat
    57001 agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact tactgtaccg
    57061 atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc aaggatacct
    57121 aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg cttggacaac
    57181 tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag aagaatggtt
    57241 tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac catgatcagt
    57301 agggagacat agatgatccg aaacaaatga taaccagata ggattccttg ctttggatct
    57361 cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc ataagataaa
    57421 gtgattgtcg ttaccatatt gacccataaa atctgtactg gggacaaagg tagcgggcga
    57481 ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg caagaagaaa
    57541 ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc ttttgcaatc
    57601 gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac ttcactacct
    57661 ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc atttacgccg
    57721 tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat tcgcagttta
    57781 tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat caactcttca
    57841 tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt taagccaatt
    57901 tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt cactgagatc
    57961 cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg atcgatcaat
    58021 ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc ctgtttcgaa
    58081 tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa ttctgcagct
    58141 gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa taatacttct
    58201 ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt tgccatatat
    58261 ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag attggctgtt
    58321 tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc tgttttctgt
    58381 tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca ggcttcttct
    58441 tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt cttatctgaa
    58501 cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat gatcgccttt
    58561 tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc tggcaatcct
    58621 tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaagaatatg ccaatccata
    58681 ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat gccttgaaaa
    58741 atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg ttcgaccgat
    58801 tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac aacgacaccg
    58861 actgcagaac cagattgtac aagcgttcct gaaaaaccca tatttttctg gtcgccgatc
    58921 gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc tcccgtcaaa
    58981 atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc tggaacgaca
    59041 tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg gattttttct
    59101 tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa accattcaat
    59161 gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat cactacgaat
    59221 aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga ggcaatgatc
    59281 aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg ttttgtctgc
    59341 tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg ttcttcttgc
    59401 gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt caatctgtac
    59461 cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg gaagtttttt
    59521 caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa tgtatctggg
    59581 cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat acaaaccaga
    59641 taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg
    59701 aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc cgtttctttt
    59761 ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga aaacaactcg
    59821 ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa aaatgtagac
    59881 aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt acgcctttct
    59941 tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga tataaaatcc
    60001 tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat
    60061 tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact aaaggacttt
    60121 cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat
    60181 agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc ttttgcggat
    60241 cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg aataaaacca
    60301 taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg atctgtccaa
    60361 aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat cctataccca
    60421 ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac
    60481 caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc
    60541 ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt
    60601 ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact
    60661 ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa
    60721 ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc aaataaacga
    60781 gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc
    60841 caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg
    60901 ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc
    60961 caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg
    61021 gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaat
    61081 ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct
    61141 ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact
    61201 gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc
    61261 aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg
    61321 acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg
    61381 aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag
    61441 gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca
    61501 ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc
    61561 aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg
    61621 aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggattttcc
    61681 aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca
    61741 aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg
    61801 cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc
    61861 atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc
    61921 atatcaaatt tcaaattcaa aaaagtgatg atccaataaa cgactaaaat gacaactaat
    61981 gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagcg
    62041 agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca
    62101 acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg
    62161 ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta caataatact
    62221 gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt
    62281 acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat
    62341 gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt
    62401 ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat
    62461 caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc
    62521 caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg
    62581 ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat
    62641 tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt
    62701 ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat
    62761 agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat
    62821 tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga
    62881 agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt
    62941 ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt
    63001 gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt
    63061 aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat
    63121 caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc
    63181 ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt
    63241 tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg
    63301 tttgattacg attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt
    63361 gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac
    63421 gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa
    63481 ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg
    63541 taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga ttttatctac
    63601 actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat
    63661 tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc
    63721 ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat ctaacgcctt
    63781 catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt ggatattttt
    63841 tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt
    63901 catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac
    63961 tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac gggcgtacca
    64021 taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat
    64081 attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga
    64141 agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc
    64201 aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat
    64261 cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt
    64321 ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat
    64381 ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac
    64441 tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt
    64501 tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat ctgtaacacc
    64561 tcttcactat agcgccaaaa atgagaattt tttttttata acatcaaatc cacgtagatt
    64621 tttacaaaca aaaataaata aagtcggatt tttctgataa ttcaacgcaa aaaagcgaac
    64681 aatttttcaa taaatcagtc ttctaaaata gataacctaa aaaaagcgaa caaacaaata
    64741 aaatgactgt ccttcattca ctaaacatat ttttattttt ttatattgtt aaaaattcgc
    64801 aatttatttt ttaatagaat tatctgtttt ccaaatttta taacataaaa aacctttttt
    64861 aacggcattt atatgctttt tcagaaatta actgatgatt tcaaaaagaa aacatgtcat
    64921 aatatttccc atattgtgca aaaaaataca aggcggaggc tctactatga ttgaagaata
    64981 tattgagaaa aatattttaa gacagctgtt tttatgtggg caattctatg taaataaaga
    65041 agtcaatcta gaaaagctct ccaatctcct gcatgtttgc aaaaccactt tactcaatga
    65101 tatcaataac ataaaaaaag aatttgaaga acagatcgct tatacacatc gggaaaagga
    65161 ccgttacact ttatattttt cagaacatat acctcgatgc aagatcatgc agcaattgtc
    65221 tcaaaattcg ttgtttttaa aaacttgtct gctttatttg gaagaagatg aacctgatta
    65281 tcttcaatta actgaatgtg aattcatctc tgtcagcaaa gcttattcat taaaaaaaca
    65341 agtattagct tattttaatg actgcgggat cgaaatcgac cgctactccc ctcgattcac
    65401 tgaaatggaa agacgtctgc tgcttcttaa tgtgtcgtat cgtttaggcg gttttaattc
    65461 ttgggagcta cctgaatcgt tttttgaacg tgccgatcgt ttcattgaat ccgtaacgga
    65521 aaatagtggc agattttatg ataaagaaaa taaagaaatc ttatcgattg gttttgctat
    65581 cagtttccta cgccagcaag tgtgtgctgt gacaatagat tcaaaattta tcgaagaaat
    65641 caaaaaacgt cctatttaca attatgtcga atcagcatgg gaaaacactg atttccaaac
    65701 ctattataaa aaagaagaat tcgcctttat tttgacacta ttcaatcttt gtaattatgg
    65761 tttccattct tatcagttga ttgcggaaga ctttcaacag ctacaccaag tgtttattga
    65821 caatactccc gaaataaaag aactagttgc tacctttgaa tctcacttca atcaagaatt
    65881 attcggaaac caaccgttcg aacgtgcgct gattcatctg atgcgttcgg cttgggataa
    65941 ctatcaatta ttcatgccag aaaaatttta tcttttaaat gaggaacaga caaatttata
    66001 taaagaagtc cagacaattt tttccagctg gtccagccag ttaccttatg atctacggtt
    66061 gaatcctaat tgcatgcggg cttttgtgat cgaattgagc ggaattctcc gtctgacaaa
    66121 agaacatctg actatctata tcgtcacaaa ttcggatgtt cattatctga tttatcggga
    66181 agccttggaa gctgtaacta ctttcgattt tcaagttgca ccaacaattt attcaagtat
    66241 atctgatata aagaagtatg cgcagcagtc ctcaaaccgg gtattatgtg aacgtacatt
    66301 atatacacca gatgcggtcc aatatgaaaa tattattcct atttcgatca atacgatcga
    66361 tcgagctatc atttcagctg tgcaaaacaa ataaggggct gtgacaaaaa tcatgtcaca
    66421 gcctcttatt tgttgaagat gcccatttct gtaacaatct cttgatacac gtcaggctca
    66481 caacctcact tattttggac atctacgggt ccgatctctt tatttaaaaa ataagaaatg
    66541 atggttcgga taactaccaa cgccgctagt tggaaaatat cctgcaaggt aggtttgata
    66601 atcgattcga tgatcccagc tgaaacaaga atttccaaac ttagaagtat atagcctccc
    66661 aacatctttt taatagcatt gttctcgctg actcggtcgc tttgcacttt tgtatggaca
    66721 aataaatatt tcaaaaaatc cttgattgat agacaaacac cccaaaccaa taaacagata
    66781 gcaaagatat caagtcccag tacaagtttt tcgaaatatg gaagaagaaa ctgcatcaac
    66841 tctgtcgtca tcctacctct cctcttcttt gtttttcttt gtcttctatt tcctgattca
    66901 agaaataaga aatgactgtt cgaatgataa taataagtgc aagcttcaaa atatctgtcc
    66961 aagtcggctt gataactgac tcgatgatat cagcaacgac tagtacttcc aagcttaaca
    67021 aaatataact tgctagcagt tttcttgtat ctgcattacg agtattacgt tctgcatagt
    67081 ttttcttgac atgagaccaa tcaaaaagtt tgaatagaac aagaacgatc ccgacaatta
    67141 agataccgat agaacaaata tttaaaatag caatgatcca ttctagaaat tcaattaaat
    67201 ttgagcgaaa catatacgct acctactttc ttttaatttg ttcctattct tttattgtaa
    67261 aagaaagtta ccttgttcgt ctatttaaaa gttgttctaa tgataacggt tcgcttaaat
    67321 ttttcttttt cactctccca tttttctccc tccatccata aatcgtggaa gtatccaaaa
    67381 caacgtagca agttaaaata aggcttagtc cgtcatcagc ttttcaaaga agcactgtct
    67441 cggtgctttt tttgtaccca aatttctaaa aatagaacca tacacctcct caaatctttt
    67501 cttcttacct accctatttt tatttacagg aaaatcagac acttcttctt ttttgtcaaa
    67561 atttcctttt tcttctcgtc ctatcatttg gcattctaat aaaaacgaac tatccttgga
    67621 tatagaaaga gaaaacaagg aggcgttttt catgagtaaa aaattaatca aagtcggcat
    67681 cggtttaggt ttgcttgccc ttggcgctgc atatctagga aaaaagacag gactattcga
    67741 agatgacagt catttatatg atgaattcga atcaatctag catcctattt agttgaatca
    67801 ctacggacat acttggaaga cattggagga atcacaatga gaaaaagtaa agcaataatg
    67861 attggtgccg gtattgccaa tatggcagcg gctgtttatt tgattcaaga aggcaaatgg
    67921 aaaggggatc agatcacctt ttattcattg gatgatcacg gttcaaatga tggtgcaccg
    67981 accgacactg tagtggatga atattggaat aaaaaccatc cattagaaaa ccaaaaaggc
    68041 tatattgctc gaggcggtcg catgcttaat taccgtacct atgtagattt gatggatctg
    68101 ttaagtcgaa taccttctgt tactgagcca gggatgactg ctgaagaaga tacgagagat
    68161 tttgatgcaa aacaccgaac ctttgacaaa gcacgtttga tggaaggtgg caaaggaatc
    68221 attgatggcg gaaaactggg attcaataat aaagaccgta ttcttctgac taaactgatt
    68281 gctatgcctg attcagaaga agaaaaacta gacaatatca ctatcgaaga atatttcaaa
    68341 gatgatccac acttttttga aacaaacttc tggtttatgt gggaaacaac atttgctttc
    68401 agaacacgta gttctgctca agaattgcgt cgctatatgc atcaaatgat ttatgaattc
    68461 actcaaatcg aacatctagt gggcgtcaat cgaacacgtt acaatcaata cgaaagtatt
    68521 atgcttcctt tgatcgagta cttgaaagaa caaggatgca aaatcatttt gaaccgccgt
    68581 gtcactgact gggaattcaa agaaacaccg atgcaagatg aaatcactgt gactggtttg
    68641 aaaattacca atacactgac tcaagaagaa gaatacgttg tggtcgatga agatacagca
    68701 gttattttca ctaatgggtc cattacagat tcagctactt taggagatta cgaaacacca
    68761 gctgtagaaa atatggatta tggtgcagct tctagtctct ggaaaaaagc cagtgaacgt
    68821 ttctacaact tgggaaatcc tgacaaattc tttgctgatc gagatgcaag cgaatgggtc
    68881 agctttaccc tgacgacgaa aaaccatttg ttgttgaatg aaatcacccg tatcacgact
    68941 caagaaccag gaaatgcctt gaattcattt atttcaacca caccgatcac gccgttaggc
    69001 caaaaagatg tcaatatgtc tatcgtcgtg catcatcaac cgcattttac gtctcagaaa
    69061 ccaaatgaaa cagttatttg gggttatttc ctttatccaa gacgacgtgg cgaatttgtg
    69121 gataaacagt atattaaaat gaatggaaaa gaaatgctag aagaactgat tggacaactt
    69181 tcaaaagtag atcctggacc agtgaatatt cgtgaaaaag aaacagaaat tttcgacagc
    69241 gtaataaata atatccctgt ctatatgcct tatgcttccg cgttgttcaa taatcgtgcc
    69301 aaaagcgatc gaccaaaagt cattcctaaa cattcgacca atctggcctt tacaggagaa
    69361 tttgtcgagc agccttatca gatgatcttt actgaacaaa gtgctgtacg ttctggagaa
    69421 atcgctgctt tccattttgc gggtattcct atgtcccgtc ttgtcaaaac accgcgttac
    69481 gacaaagata ttcctactct agctcgtgcc gcaaaaaaaa tgtttgaata atcaagaggt
    69541 tgtgaaaaag ctgccctgca tcaagtaata agaacaacca ggctaaaaca gcttcttatg
    69601 tttttgattt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
    69661 ggttgtgaaa aagctgccct gcatcaagta ataagaacac ccaagctaaa actgcttctt
    69721 atgtttttga cttggttgga cttattaccg cagatgcaag cttttgaaca ccatttacca
    69781 agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt
    69841 cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta
    69901 ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg
    69961 ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa
    70021 cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc
    70081 ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa
    70141 aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga
    70201 aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta
    70261 ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt
    70321 ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa
    70381 cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc
    70441 atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg
    70501 ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt
    70561 ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg
    70621 aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc
    70681 attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg
    70741 taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag
    70801 cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt
    70861 ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa
    70921 tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa
    70981 cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg
    71041 gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag
    71101 cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag
    71161 ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt
    71221 acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta
    71281 tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa
    71341 tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa
    71401 aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga
    71461 ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag
    71521 atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct
    71581 cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa
    71641 ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga
    71701 ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga
    71761 agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca
    71821 ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt
    71881 caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt
    71941 ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc
    72001 ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg
    72061 gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa
    72121 gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc
    72181 atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa
    72241 ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta
    72301 aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag
    72361 tgatgtaaaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat
    72421 ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca
    72481 aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca
    72541 atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta
    72601 aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg
    72661 gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat
    72721 tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc
    72781 ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa
    72841 agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgcccagct gcgattgcat
    72901 taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg
    72961 ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg
    73021 gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga
    73081 tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga
    73141 tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca
    73201 ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc
    73261 caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga
    73321 agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta
    73381 aatgataagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag
    73441 cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga
    73501 acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca
    73561 tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg
    73621 ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt
    73681 ttctttactg tagattacgc gttcaatcga cgatacagat caatgatctc ttttgctaaa
    73741 tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact
    73801 tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg
    73861 actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc
    73921 gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc
    73981 atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta
    74041 tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc
    74101 agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt
    74161 ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg
    74221 attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc
    74281 tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat
    74341 gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg tatataagca
    74401 agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat
    74461 accaagttgc atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat
    74521 tacctttttt cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt
    74581 ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
    74641 cataagatgt tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca
    74701 acctctcaat aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt
    74761 cacaacctct tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta
    74821 tttatttata gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc
    74881 acgaagcaga ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc
    74941 catatcgatt cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat
    75001 agccttcgca atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga
    75061 taaattcgat acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat
    75121 attctcttcc cataccgttt tctgttatca gccaagggat attatgataa ttttctttta
    75181 gatacatggc tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca
    75241 ttttcttccc aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt
    75301 cattccattg ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga
    75361 aatctactgt atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg
    75421 attgtttttg acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa
    75481 aaaatactcg attataaaac aaatcataca tataagcagc tttttggtct tctggagaat
    75541 tggatcgagc atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc
    75601 cgctgcggta aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca
    75661 tccacttttt tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt
    75721 ggggaacgat tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa
    75781 aggcaatttg agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat
    75841 atttttccaa aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt
    75901 ctgcttcgat caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta
    75961 tacagttctc ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca
    76021 gatggatgga tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta
    76081 cggctgggcc atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa
    76141 acgaatcctg tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag
    76201 caccaagaat aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc
    76261 ccatcatttt ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca
    76321 ataatacaaa caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca
    76381 ttgacgatcg gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa
    76441 gcggttgcaa aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct
    76501 aaaattggat tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc
    76561 ccaggagcaa gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat
    76621 actgcaatca gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa
    76681 aatggcatag taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca
    76741 gcaattgccg ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata
    76801 ccaatcaccc aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt
    76861 acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct
    76921 gtaagttctt tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg
    76981 aataaagcag aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt
    77041 agtttcttct gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt
    77101 cccaaaaata accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca
    77161 gtctgatttt cattcactaa ttcataaaca gttggattaa cagataaata agacatcact
    77221 gcgatgatac cagggaatac accatccact ttattcagct tggctaattc gatccctatc
    77281 agaaaaacag catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata
    77341 ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca
    77401 aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca
    77461 gcaaatgcgt tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc
    77521 cctaaaacat tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct
    77581 tctccttgac gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa
    77641 ctatttactc ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata
    77701 aaatacatca gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc
    77761 tccgatttat tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt
    77821 cgatggcctg aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat
    77881 cataattttt attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg
    77941 ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt
    78001 cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga
    78061 tttgattctt cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc
    78121 tcagttcaaa taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg
    78181 ggtcattggc aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa
    78241 ggatattcct ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta
    78301 aataatcgga caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct
    78361 ttaaatatct aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac
    78421 cacatacctc cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat
    78481 ctgatgattt tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga
    78541 tatttaccat aaaaaagact agga
//
LOCUS       DAZBWR010000002        78211 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000002 DAZBWR010000000
VERSION     DAZBWR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.002"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..413
                     /locus_tag="ACHGT5_000074"
     CDS             147..413
                     /locus_tag="ACHGT5_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN7216097.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            410..841
                     /gene="ruvX"
                     /locus_tag="ACHGT5_000075"
     CDS             410..841
                     /gene="ruvX"
                     /locus_tag="ACHGT5_000075"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN7216098.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            866..1171
                     /locus_tag="ACHGT5_000076"
     CDS             866..1171
                     /locus_tag="ACHGT5_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN7216099.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1252..2397)
                     /locus_tag="ACHGT5_000077"
     CDS             complement(1252..2397)
                     /locus_tag="ACHGT5_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7216100.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(2458..3105)
                     /locus_tag="ACHGT5_000078"
     CDS             complement(2458..3105)
                     /locus_tag="ACHGT5_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7216101.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            3299..3592
                     /locus_tag="ACHGT5_000079"
     CDS             3299..3592
                     /locus_tag="ACHGT5_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216102.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            3636..3947
                     /locus_tag="ACHGT5_000080"
     CDS             3636..3947
                     /locus_tag="ACHGT5_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216103.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            3944..4568
                     /locus_tag="ACHGT5_000081"
                     /pseudo
     CDS             3944..4568
                     /locus_tag="ACHGT5_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            4709..4867
                     /locus_tag="ACHGT5_000082"
     CDS             4709..4867
                     /locus_tag="ACHGT5_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216104.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            4868..5230
                     /locus_tag="ACHGT5_000083"
     CDS             4868..5230
                     /locus_tag="ACHGT5_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216105.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            5267..6115
                     /locus_tag="ACHGT5_000084"
     CDS             5267..6115
                     /locus_tag="ACHGT5_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN7216106.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            6105..7580
                     /locus_tag="ACHGT5_000085"
     CDS             6105..7580
                     /locus_tag="ACHGT5_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN7216107.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            7846..8253
                     /locus_tag="ACHGT5_000086"
     CDS             7846..8253
                     /locus_tag="ACHGT5_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN7216108.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            8256..8471
                     /locus_tag="ACHGT5_000087"
     CDS             8256..8471
                     /locus_tag="ACHGT5_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216109.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            8475..8855
                     /locus_tag="ACHGT5_000088"
     CDS             8475..8855
                     /locus_tag="ACHGT5_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN7216110.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            8989..9144
                     /locus_tag="ACHGT5_000089"
     CDS             8989..9144
                     /locus_tag="ACHGT5_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN7216111.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            9213..9686
                     /locus_tag="ACHGT5_000090"
     CDS             9213..9686
                     /locus_tag="ACHGT5_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN7216112.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            9683..11377
                     /locus_tag="ACHGT5_000091"
     CDS             9683..11377
                     /locus_tag="ACHGT5_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN7216113.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            11343..11528
                     /locus_tag="ACHGT5_000092"
     CDS             11343..11528
                     /locus_tag="ACHGT5_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216114.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            11532..12707
                     /locus_tag="ACHGT5_000093"
     CDS             11532..12707
                     /locus_tag="ACHGT5_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN7216115.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            12700..14223
                     /locus_tag="ACHGT5_000094"
     CDS             12700..14223
                     /locus_tag="ACHGT5_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN7216116.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            14279..14563
                     /locus_tag="ACHGT5_000095"
     CDS             14279..14563
                     /locus_tag="ACHGT5_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN7216117.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            14550..14885
                     /locus_tag="ACHGT5_000096"
     CDS             14550..14885
                     /locus_tag="ACHGT5_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN7216118.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            15180..15806
                     /locus_tag="ACHGT5_000097"
     CDS             15180..15806
                     /locus_tag="ACHGT5_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216119.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            16548..17384
                     /locus_tag="ACHGT5_000098"
     CDS             16548..17384
                     /locus_tag="ACHGT5_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN7216120.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            17448..17879
                     /locus_tag="ACHGT5_000099"
     CDS             17448..17879
                     /locus_tag="ACHGT5_000099"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216121.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(17941..19263)
                     /locus_tag="ACHGT5_000100"
     CDS             complement(17941..19263)
                     /locus_tag="ACHGT5_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN7216122.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(19417..21057)
                     /locus_tag="ACHGT5_000101"
     CDS             complement(19417..21057)
                     /locus_tag="ACHGT5_000101"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN7216123.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            21390..21860
                     /locus_tag="ACHGT5_000102"
     CDS             21390..21860
                     /locus_tag="ACHGT5_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN7216124.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            22082..23185
                     /locus_tag="ACHGT5_000103"
     CDS             22082..23185
                     /locus_tag="ACHGT5_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN7216125.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            23469..24149
                     /locus_tag="ACHGT5_000104"
     CDS             23469..24149
                     /locus_tag="ACHGT5_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN7216126.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24239..25273
                     /locus_tag="ACHGT5_000105"
     CDS             24239..25273
                     /locus_tag="ACHGT5_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN7216127.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25287..25622
                     /locus_tag="ACHGT5_000106"
     CDS             25287..25622
                     /locus_tag="ACHGT5_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216128.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25606..28086
                     /locus_tag="ACHGT5_000107"
     CDS             25606..28086
                     /locus_tag="ACHGT5_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216129.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            28142..29701
                     /locus_tag="ACHGT5_000108"
     CDS             28142..29701
                     /locus_tag="ACHGT5_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216130.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29698..31062
                     /locus_tag="ACHGT5_000109"
     CDS             29698..31062
                     /locus_tag="ACHGT5_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN7216131.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            31233..32303
                     /locus_tag="ACHGT5_000110"
     CDS             31233..32303
                     /locus_tag="ACHGT5_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216132.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            32318..32986
                     /locus_tag="ACHGT5_000111"
     CDS             32318..32986
                     /locus_tag="ACHGT5_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216133.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            33006..33584
                     /locus_tag="ACHGT5_000112"
     CDS             33006..33584
                     /locus_tag="ACHGT5_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7216134.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            33985..34896
                     /locus_tag="ACHGT5_000113"
     CDS             33985..34896
                     /locus_tag="ACHGT5_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN7216135.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(34984..35901)
                     /gene="rnhC"
                     /locus_tag="ACHGT5_000114"
     CDS             complement(34984..35901)
                     /gene="rnhC"
                     /locus_tag="ACHGT5_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN7216136.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            36103..36540
                     /gene="zapA"
                     /locus_tag="ACHGT5_000115"
     CDS             36103..36540
                     /gene="zapA"
                     /locus_tag="ACHGT5_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN7216137.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            36599..37147
                     /locus_tag="ACHGT5_000116"
     CDS             36599..37147
                     /locus_tag="ACHGT5_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFN7216138.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            37262..39622
                     /locus_tag="ACHGT5_000117"
     CDS             37262..39622
                     /locus_tag="ACHGT5_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN7216139.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            39709..40023
                     /gene="trxA"
                     /locus_tag="ACHGT5_000118"
     CDS             39709..40023
                     /gene="trxA"
                     /locus_tag="ACHGT5_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN7216140.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            40205..41983
                     /gene="uvrC"
                     /locus_tag="ACHGT5_000119"
     CDS             40205..41983
                     /gene="uvrC"
                     /locus_tag="ACHGT5_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN7216141.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            42128..43027
                     /locus_tag="ACHGT5_000120"
     CDS             42128..43027
                     /locus_tag="ACHGT5_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216142.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            43020..44282
                     /locus_tag="ACHGT5_000121"
     CDS             43020..44282
                     /locus_tag="ACHGT5_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216143.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            44443..46341
                     /locus_tag="ACHGT5_000122"
     CDS             44443..46341
                     /locus_tag="ACHGT5_000122"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN7216144.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(46671..47390)
                     /locus_tag="ACHGT5_000123"
     CDS             complement(46671..47390)
                     /locus_tag="ACHGT5_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN7216145.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    47580..47627
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            47727..48059
                     /locus_tag="ACHGT5_000124"
     CDS             47727..48059
                     /locus_tag="ACHGT5_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216146.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            48046..50049
                     /locus_tag="ACHGT5_000125"
     CDS             48046..50049
                     /locus_tag="ACHGT5_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN7216147.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            50068..50538
                     /locus_tag="ACHGT5_000126"
     CDS             50068..50538
                     /locus_tag="ACHGT5_000126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN7216148.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            50559..51143
                     /locus_tag="ACHGT5_000127"
     CDS             50559..51143
                     /locus_tag="ACHGT5_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFN7216149.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            51158..52144
                     /locus_tag="ACHGT5_000128"
     CDS             51158..52144
                     /locus_tag="ACHGT5_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN7216150.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            52134..52445
                     /locus_tag="ACHGT5_000129"
     CDS             52134..52445
                     /locus_tag="ACHGT5_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN7216151.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            52467..54248
                     /locus_tag="ACHGT5_000130"
     CDS             52467..54248
                     /locus_tag="ACHGT5_000130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN7216152.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            54241..55617
                     /locus_tag="ACHGT5_000131"
     CDS             54241..55617
                     /locus_tag="ACHGT5_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN7216153.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            55624..56259
                     /locus_tag="ACHGT5_000132"
     CDS             55624..56259
                     /locus_tag="ACHGT5_000132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN7216154.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            56289..57644
                     /locus_tag="ACHGT5_000133"
     CDS             56289..57644
                     /locus_tag="ACHGT5_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN7216155.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(57836..59230)
                     /locus_tag="ACHGT5_000134"
     CDS             complement(57836..59230)
                     /locus_tag="ACHGT5_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN7216156.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            59322..60293
                     /locus_tag="ACHGT5_000135"
     CDS             59322..60293
                     /locus_tag="ACHGT5_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN7216157.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            60290..61072
                     /locus_tag="ACHGT5_000136"
     CDS             60290..61072
                     /locus_tag="ACHGT5_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN7216158.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(61241..61612)
                     /locus_tag="ACHGT5_000137"
     CDS             complement(61241..61612)
                     /locus_tag="ACHGT5_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7216159.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            61746..62189
                     /locus_tag="ACHGT5_000138"
     CDS             61746..62189
                     /locus_tag="ACHGT5_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN7216160.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(62232..63041)
                     /gene="yidA"
                     /locus_tag="ACHGT5_000139"
     CDS             complement(62232..63041)
                     /gene="yidA"
                     /locus_tag="ACHGT5_000139"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN7216161.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(63060..64427)
                     /locus_tag="ACHGT5_000140"
     CDS             complement(63060..64427)
                     /locus_tag="ACHGT5_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN7216162.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(64411..65241)
                     /locus_tag="ACHGT5_000141"
     CDS             complement(64411..65241)
                     /locus_tag="ACHGT5_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN7216163.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            65404..65841
                     /locus_tag="ACHGT5_000142"
     CDS             65404..65841
                     /locus_tag="ACHGT5_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN7216164.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            65917..66537
                     /gene="rpoE"
                     /locus_tag="ACHGT5_000143"
     CDS             65917..66537
                     /gene="rpoE"
                     /locus_tag="ACHGT5_000143"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN7216165.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            66726..67232
                     /locus_tag="ACHGT5_000144"
     CDS             66726..67232
                     /locus_tag="ACHGT5_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN7216166.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            67249..68148
                     /locus_tag="ACHGT5_000145"
     CDS             67249..68148
                     /locus_tag="ACHGT5_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN7216167.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            68235..69119
                     /locus_tag="ACHGT5_000146"
     CDS             68235..69119
                     /locus_tag="ACHGT5_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN7216168.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(69311..69382)
                     /locus_tag="ACHGT5_000147"
     tRNA            complement(69311..69382)
                     /locus_tag="ACHGT5_000147"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69347..69349),aa:Arg,seq:ccg)
     gene            69442..70776
                     /gene="rpoN"
                     /locus_tag="ACHGT5_000148"
     CDS             69442..70776
                     /gene="rpoN"
                     /locus_tag="ACHGT5_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN7216169.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            70925..72016
                     /locus_tag="ACHGT5_000149"
     CDS             70925..72016
                     /locus_tag="ACHGT5_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN7216170.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            72057..73058
                     /gene="gap"
                     /locus_tag="ACHGT5_000150"
     CDS             72057..73058
                     /gene="gap"
                     /locus_tag="ACHGT5_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN7216171.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            73168..74358
                     /gene="pgk"
                     /locus_tag="ACHGT5_000151"
     CDS             73168..74358
                     /gene="pgk"
                     /locus_tag="ACHGT5_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN7216172.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            74462..75217
                     /gene="tpiA"
                     /locus_tag="ACHGT5_000152"
     CDS             74462..75217
                     /gene="tpiA"
                     /locus_tag="ACHGT5_000152"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN7216173.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            75333..76631
                     /gene="eno"
                     /locus_tag="ACHGT5_000153"
     CDS             75333..76631
                     /gene="eno"
                     /locus_tag="ACHGT5_000153"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN7216174.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            77030..78169
                     /locus_tag="ACHGT5_000154"
     CDS             77030..78169
                     /locus_tag="ACHGT5_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN7216175.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        26103 a        13441 c        16425 g        22242 t
ORIGIN      
        1 cagaaaaaag gaaaaaaaca tacaaatagt gtttcttaat ttattcggaa gtttggaata
       61 tttgtttgta gttttccctt tcttcgagta aaataaggtt gtagaaggta agctggaatt
      121 accttgcggg aaagaggtgt agaatcatgg gttttacaga tgaaaccgta cgttttggtt
      181 ttgacgatag tcgcaaaaaa gaagtgagcg aaacattagc gattgtttat cgggctttgg
      241 aagaaaaagg ctacaatcca atcaatcaga ttgtcggcta cttgctttct ggagacccgt
      301 cctacattcc tcgttatcgt gatgctcgaa atttgattcg ccgtcacgaa cgcgatgaga
      361 tcatggaagt aattgtcaag gactacctct caaatcatgg agtgaattta tgagagtaat
      421 gggcttggat gtcggttcta agacagtagg tgttgctatc agtgacccct ttggctggac
      481 agctcaagga gtagagatca ttcggatcaa tgaagcagaa gaagagtttg gctttgatcg
      541 gttgaaggaa ttagtagcac aatatgaagt agaccggttt gtagttggat tacctaaaaa
      601 tatgaacaat tccatcggac caagggcaga agcttccatg tcatacggag aaaagatcaa
      661 agaactattc tccttgccag ttgattatca agatgaacga ttaaccactg ttcaggcaga
      721 acgtatgcta gtagagcagg caaatacttc aagagccaaa cgaaaaaaag tcatcgataa
      781 attagcagct gtgatgatct tacaaaatta tttagacggc tcaggtaagc aattatttta
      841 ataataaaag aaaagaggta atcttatgac agaacataac cattcccacg accatgaagg
      901 acacgaacac atcacattgg tggatgacca aggaaatgaa acattatatg aaatcctact
      961 aacagttgac ggacaagaag aatttggacg taactatgtt cttttatatc ctgctggtgt
     1021 ttcagaagat gaagatgtag aattacaagc ttatgcatac atcgaaaacg aagatggaac
     1081 agaaggcgag ttagaacaaa tcgaaacaga cgcagaatgg gacatgattg aagaagtatt
     1141 caatacattc atggcggaag aagaagaata gactataaag ctaggagaaa ccttgatatg
     1201 atagggtttc tccttttttt attttttact gtatgccata ttgtatgcca tctagtcaat
     1261 atttaagtgt tgtactaatt tttcaacagt cttttctttc gtttcttttg ccaagtgacc
     1321 gtaacaatcg atcgtctgac taatttttgt atgccgtaat ctagtttgaa cttctaccat
     1381 agtagctcca gcgtccaaga gcattgaagc atgagtgtgt cttgctacat gcacaccgag
     1441 aaatggaacc atagcttttt cacaaatagt aatcaatctt gcataaatag cgtggtgtgt
     1501 gattagttct ttatagatac cacaaaaaat catagtcgga tcagttacac caagtgtcag
     1561 catatatttc cgctgattaa tctgccattt ttttaatata gaaattgttc tttcatctag
     1621 tggaagaacc ccagcactac aatcagtttt aggatcactg attttatagc catttttgga
     1681 ttggcttagt gttttattga tggtgaccgt attttcttga aaatcaatgt ctgaccaatt
     1741 caatgcaagg gcttcactga tcctaacccc gctataaaag agaaagcgta gtaacgtttt
     1801 atcaaagtcg tttcgataag tttcaggttt gctgtttagg taatcaaaca aggtgatcag
     1861 ttgttcttta ctgtaaactt tgaccttttc tttatgctta gtggtggttc ttttaggcat
     1921 taatacgttg tctagtggat tagtctcaat aatcccaaga ttgatagcgt agctaaaaat
     1981 acgcttagaa gttgaatgaa gtaccttgta ttggtcagta gtttcagccc agctatttac
     2041 aatttgttgg gcttttttta ccgtgacttc attcacacga agatttccat attttggaag
     2101 aatccacttt ctaaacttga tttcagtttt catgaaggtg gcttctcgga cagtggtttt
     2161 atatgtttct agccacaagt cataaacttc tttaaacgtt tcgttgttcg cttttttaat
     2221 accgttttta tcgtagtcca cttgcaagcg tgacaaggca agctgggctt ctttttttgt
     2281 tttgtaacct cgtctacgaa ccttatcagg tttgccagtc attttgttca ttccaagata
     2341 ggcttcatag agccaaaatt tacctttttt agtttcatat tgcttgaatg ttgccataat
     2401 agttttccct ccatagtgta gcaagccgtg ggggcggttg gaggaggcag tttattatta
     2461 ttttgaatag tgtttattta gctcagtaac aagagctgtg aactctttta gaagatcatt
     2521 ttcataaaat tcttgatact cttttttctc atctgtattt ttttctgaat cattaattaa
     2581 tgagtcatat cgtaaaagcg tatttagaag agctgcgaga taagcaattg gggtactccc
     2641 gtcaggaaat tcaattcgag atcctgtttt aagatatttt attgcgtttg ttaaataaat
     2701 cttctgtgag acagtcaggt tatcttcttc gatgttattg atagcattag aaagattaga
     2761 atcaattata gagttatttt tttcaatagt ttttttcaat tgcttttcaa gcacatttcg
     2821 cttaatttcg ggcatcatat gcatatctct taatgtataa gatccgttta atagataggt
     2881 agtggataca tctaatacct ttgaaagttg tttaagcctg gtgttattgg gaagattata
     2941 gccattttcc caattactaa cggcaccttt actggccggt ggggataatt tacctccaag
     3001 ttgttccatt gagagtccct tttcaattct agccagtcta attctttgtc ctgtctccac
     3061 tgcttcgggg tatttttttc tagccacatt ttcaccacca ttcatatatt ttctctacta
     3121 tatcacgatt taaatatgtg tgaaacataa aagttcaaaa aagtatgtta aaattcttgc
     3181 aaatgaaaag gtatgatggt atattgagtt tataagaggt atagaaatgt attcaagagc
     3241 ctcttgcatt tttttaaaat ctttgtatag aaatgtatgc taacgagggg ggataaatat
     3301 ggaattaaaa attgacaaac tcacgcttga tgatccagct tttaaaatta ttgacgatcg
     3361 agttgttcaa caagtagagc aaactcaagg aacgttactt tcattattag aaaaaaagta
     3421 ccagtttcca aaatatatga acaaggcaca agctgctaag tatatgaacg tttcatacaa
     3481 tactatgatg cagaaatatg ttcctaacgg tttaaagttg attatagttg atggagtcgt
     3541 aagaatttca caagatgaat gtgatcgctt tatggaagaa taccaaaaat aaacgcaagc
     3601 cgtgggggca aagggattaa taggaggaaa taataatgga atcgacacaa aataaagcaa
     3661 tcgaaaaagt attatcaaca attcttacag aagacacagc ggcagaatta gcaaacttgg
     3721 aaggcaaagc gctagaagaa acgtttgaat gtttgtacga acaaatggac tatcaaaaat
     3781 tgctaccaca agaaccaacg gtaagcggag tattgcgtgg cttgaacgat ttagtacaag
     3841 cagagtttaa agaacgactt tctattgaag agtatcaaga aattttgtat cagcaagttg
     3901 agttgttagc gagcttgcta gggcttgaac tggaggacgt tgaatgaaaa ctgaaatttg
     3961 gaacggacac attataagat ttgtcgatat taatgatgaa tggtgggcag tggcgaaaga
     4021 tgttgcggaa gcgttaggtc ttaaacaggt tactagagcc attcactctt tgcctaaaga
     4081 tggggttact actagtaagg tcatcgattc attgggcaga acacaagatg taaacatcat
     4141 caacgaaaaa aaatatttac cgcatggcat tcaaaagccg taaaaaagaa gccgaggcat
     4201 ttcaagactg gattttcgac atcatcaaag aactacgcca gtccacagga cttgaaggct
     4261 ttcaagtatt ccgcatgcta gacaaagaac accaaaaaga agcaatgact aaattaagcc
     4321 acgctattac tgaaccaaaa ccagtagact acattaaagc gaatgtgata gccaataaag
     4381 ctgtatcaac tatttatggt cattccaaaa tggtaaaaaa gaaagatatg actcctgaaa
     4441 tgttggttga tcgtgaacct attttagatg agacagtaga attaatgact gttaaagaaa
     4501 aatatgggtt gcagttttca gtgagtgaga aaatttataa tcgttctgct gaattgcaga
     4561 caacttagga gcattaacat gatcgagaac aaagaaaaag ccttatccat aacagtcttg
     4621 gcggacagga taaaggcaac ttaattggga taaaacccga tattctttgt tccgattata
     4681 ccatattaaa aaatcggagg gaaccattat gaaacgaaac ttaattatag gaagcatttt
     4741 aatcggcgga attttcggct tattaccatt aattgcaaaa atagctatct ttgcaggatt
     4801 agcgttatat ctcttcactg cttatgacga gtacgactat caattaagaa ctggagggca
     4861 taagtagatg tacaaaacaa aactattaaa ccaactcgat agccttgaat tagaggaaat
     4921 taatcaaggg atcgctgaac tagaaaataa tatcgggaaa acctactttg gaaattcatt
     4981 caatgaaaaa ctaacagttt tgtatgtact caaaaaacat gcagaacaca aaataatttg
     5041 tcgagaaatc aacgagctaa aaaatcagat tttaaccgca tggttaaaca ttactgatat
     5101 gcaagaagca agggtgaaaa catttaatac atgggtaaag tatcagaatc aattaaaagg
     5161 tgctgaattt gttcgggatg ggttgaaata cgaacttgag cagttgaagc tcatggaggt
     5221 gtctgaatga tttataccag tattttgaaa aaggaggaga ggtttattgg caaataatta
     5281 tcaagcagca acggaattac aaaagtcggg atggtcaatt tatcccttat ctcctggaac
     5341 taaaattcca ataaaaggct ctagtggttg gaatgatgca actaacgatt ctaatcaaat
     5401 taatgaatgg tggacggaag atgcaactag aaatattggg ttaatgcttg aagatcatca
     5461 aatggtggta gttgacctag atcaacattc tgaaacacga aacggcgttg agaattataa
     5521 aaaacttgct tcaatgtatg atccatttcc ttctacttat acggaaatca caccaggtaa
     5581 aggaatccat ttcttcttta aaattcctga tggtgtaatt atccaacaag aaacaggtgc
     5641 atttgctgat atcttcgggc gtgatgataa aggtaaaagt ttaagcggga ttgatattat
     5701 cactaaagga gttccaatag cgccaactat gattatttca agaagtgttg ggagagcata
     5761 tgaacctatt gaaaacgtat ctctaaataa tattgcacca gctcccgatt ggctagtgaa
     5821 cctactgcta aaaaaaccgc aagaaataaa gactcaatac cgacccgtag caaaaacagg
     5881 gacagcaaaa aaattagata tgattgttca aggcgctggc gaaggtagta gaaacgatca
     5941 tttaactaag ttatgcggtt ggttactatg gcacggtgta gataatcaaa ctttagcaga
     6001 attgatttat acagcaaatg cgtacaatga accaccgcta aaagataaag aagtgaatca
     6061 aattattcga tcgatgatta aaaaagattt gagggggaaa gctcatggct cataagcata
     6121 aattagatag tattttagac tttcccgaag ccagcgagcg tgaagacaat atcattgaat
     6181 tgaaaacctg gatgtcacga ttaagatgca ataaagacga tcagattaaa agtaacagtg
     6241 ttgtgaatgc agaattgatt ttaaccaatg atagcaattt agctggaaca attgcttata
     6301 acgaatttag cggatacatt cacctattaa aagattctcc ttggatcaat cgtaccgctg
     6361 gagaatggga agacagtttt gaggatgcct taacggcata tattgaagaa aactacaatg
     6421 tagtttttga tgacaacaaa atacacaaag cagtagtaaa tgtggctaga aagaatgttt
     6481 tcaacccagt aaaagaacgt atcgaaaaag ttaaatggga tcaacagcct agactggaaa
     6541 cactgtttat tgacctttta ggggtagacg acaaccttta cacacgagaa gtaacaaaac
     6601 gttggatagt cggatcagtc gcacgtatct ataaaccagg aataaagttt gagatcgttc
     6661 cagttttgga tggaccgcaa gggattggta agagtaccgt tccagcatta ctttacacgg
     6721 atgatttttt caccgattca ttagattcgc taggtgaaaa gaaagatgat tatatgcagc
     6781 ttcaaggcaa tgtaattatt gaacttggag aattatcttc aatgaacaaa actaaaattg
     6841 aacagataaa aaacttcatc agtgccaaga tcgataaaat tagacctcct tatggtcgga
     6901 atgtcatagc ttgggcaagg caatgtgtat ttattggaac gtcgaatgat gggcagtatt
     6961 tgaaagatga tacaggaaac cgccgttttt atcctttgcc ttgtaagaat aagccaaaga
     7021 tgaatccgtt taaaacaaag gatgaatatt ttcttcaagt gttagctgaa gcaaaggtgc
     7081 tttttgataa aggacaacga atctatttta gtcccgatga agataaagaa gtcttagaaa
     7141 ttgcaaaaga ctatcaagag gatgccaaga tggagaatcc aattaaagaa gcaatcacta
     7201 aatatttgga aatggaaatc ccgtataaat gggaagaagc tccagcgtgg gcgagacgtt
     7261 cttattatca gaactatcct gacagtaaat gtaatgagaa aaatttacag catttctata
     7321 atggagctac tatgcaacaa gagttttatt tagttgatag cgtactcacc gctgatattt
     7381 tggaagctgt ttttgataaa caagcaaagg atttattgaa tggtcgatca gatgcagaaa
     7441 ctaaaaagat tgccttgatt attagcagta ttcctggttg ggaacggaag caactgccaa
     7501 aaagaaataa acgacgagga ttttacaata agtcaaatgc ctatgaaaat aaaaatcgag
     7561 ctggaaagta caaaaagtaa cgggtagtaa cgggtcagta acacctagcc cgttactagt
     7621 ttacccttac tgccccaagg gatacagcaa tttggtaacg gggtaacacc ttatttcctt
     7681 aaaacttttg aaatagttag ctaatatgct ggggaataat gttactggat tttacccgtt
     7741 acctgttacc aaaacgctag aacccttgtc cccgttggga ttgagtggta acgggtacct
     7801 gttacgtagg tgttaccacg tgttacgaat caaggaggaa agtaaatgct caatattatt
     7861 tcaacaaaca aagcaccgaa ctttcaatat acagatgaaa tggatcggtt tctaatgaac
     7921 acgctggcat ttagtgtggg attggtaaca gaagattatt ccacatttga tccagaagta
     7981 ttaaagataa tggaagaaga acctgattgg ctgcaggagt cagtagcgtg gtgtcaatcg
     8041 ctagtagtgg gatcattggt agatagtggc aactatgatg atacaggtga gctaatggat
     8101 gaatttaatt gtctgcttaa cctatatgat cgagcgagac aacgagagct tacatcgaat
     8161 gaggataatt tgtttttaaa catccatgat aagttcttgg ctttgctact aacagatgat
     8221 gaattgataa ctaatttatt ggaggtagaa taattatgca tatggaaagt attagatact
     8281 tcgatggtga gaaacacatt gagtattcaa acactcaaga agatgtagac tttattagtt
     8341 ttgaggaaga taaaatcact aaagttaatt ttaaagacgg aacgtgcttg aagatagttt
     8401 cgccttatat tgaatacaaa tcaaaatggg aacaagatga ttctgacgaa cgagatcgct
     8461 ttggttggta atctatggca gttaaaaaac aatgtaatca tgctggatgt aaaacattga
     8521 ttgattatag gcagaagtat tgtgggaagc ataaagcaaa gcaaacggca attaagcgtg
     8581 aagaaagaaa gcaaagcgaa ggtaaatatt ttcaattcta tcaaagtaga acatggcgaa
     8641 aggcttcata cttgtatcgg ttaaatcatc cagtatgtga ggattgccta gaagaaggct
     8701 tgataaggaa agctgatgtt gtagatcaca aaattgaact aaaagatgat tggaatagac
     8761 ggttagatga aactaatttt cgttctttat gccatgcaca ccataattcc aagacagcca
     8821 atgaaagaca aagacgagaa aagagtaccc tttgagttaa gggggcatag tgtgaaactt
     8881 actgacaatc gatgcctact cattttggta caaataaccg ttgtgaaaag gcataagggt
     8941 aattacaaat aacgattaca cttgtaatca caagtgaaaa agtatataat gaaagtagga
     9001 gatttattga aaaatacaat tgttattact gataaaacag ggaaacagcg caccattgag
     9061 ttacctcatt ttggtcaagt gaccattcaa atgcagaacg gaaaaatcat ttatatagat
     9121 aaattagata aagaaaaatt ctgatcgaaa aacgaaggaa tgtgagctta attgcttacg
     9181 ttcctttttc ttttgtctga aaggaggaca acatgggaca ccctaaaata ttacacgata
     9241 caaaaggcaa tatatcaagt gaagaaaaag caatccgtgt ggacgctaga gaagaattgt
     9301 tcaagcagca accattaata aatatcacgc cccctgactg gatggcagcg agcgctagaa
     9361 gtgaatggaa tcgtatcgta ccaacattaa aaaaagatta tccattgagt gaagcagatt
     9421 atggttcatt ggtagcctat tgtttagcct ttgctcgaat gaaaacagcc gaagccgaga
     9481 taagaaaatc aggtacgttt atcacatgcg aaaacggagt aaagaaagcc aatccagcgg
     9541 ttcgggttca atcacaagct atgagtgatt tgaaaaaaca agccacctca ctaggtatga
     9601 ccttagaatc acgatcaaaa ctagctttga acaaggttaa aaatgatgaa cccgaagacc
     9661 cgttcaaaga gttgatgaat tcatgaatga ttacattgag aaagtcctat caggtaagct
     9721 gattgcaccc aagaaaatta tccaagcgtg tgagcgtcat ataagcgatt tggagcgttc
     9781 taagtcagat agcttccctt atgtgttcga tgaagaacaa gccaccaaag cgattaaatt
     9841 cattgagttg ctaccatcta cggacggtaa agcaatcaag atgttaggat ttcaaaaatt
     9901 cattctaggc agtctttatg gctggcgtac taaagaaggg aattacaggc gattcaatcg
     9961 agcgtttacc agtatgagcc gtaagaatgg gaaaacgtat atcgcaagtg gcatggctgc
    10021 caatgcgttg attatggaac aagaaccagc agaagcaagg caagtattgt ttgtaagtaa
    10081 cgctttgaaa caagctaaat tgggctatga tatgctgtct aattcactta gaaacgtggt
    10141 caagtctagt aagtttttaa gaccacaact gaaaattatg aactctaaga ttcagcactt
    10201 gccctctaat tcgttcgcta tggcactggc tagtgaaacc agcacgctag atgggtttgc
    10261 accaacaacc gcaattcttg acgagtggca cgaagcaaaa actcgtaaaa cgtacaacgt
    10321 cattaagtca ggaatgaccc aacaaaagaa cggcttattg tgtgttatta gtaccgctgg
    10381 gcttgattta aacgttccta tgtacgagga atacttattg ttagatcgtg tgctaaaagg
    10441 cgaagaacaa gccgacaggt actttatagc gatatgggaa ttggacgatc ccgaagaaat
    10501 tcacgatcaa gagaaatgga tcaaagccaa tccgattttt gaaagtgaag aaatcaaaaa
    10561 agtaatgatt ccaaccattc aagatgatgt gaaccttgct ttgaaacaaa ataaccttaa
    10621 ttctgtattg gtgaaaaact tcaatttatg gagacaagcg agtgaggaca gctatatgat
    10681 tgctgaagac tggcaggcaa ccgaagtaga accacaagat attacaggca agcccgttta
    10741 tatcggagtg gatttatcta aaacagatga tttaactagc gtttcatgga tcgtaccgct
    10801 agataacggc gaactttatt gtgattcaca tagctttgta gccaccaaat atgggcttca
    10861 agacaaagaa aagcgcgacg gtttgcctta tcgagaacta gaaaaagctg gtgagtgttc
    10921 cattactcaa ttagaaagtg gaatcgtgga ctatgaccaa gtatttcagt ttattcaaga
    10981 tttgattcaa gaaaatgatt tggaatgtat ggggatttgt tatgacccgt ataacgctaa
    11041 ttcgcttatc agtaaagctg aaaaagccaa ctacccaatg ttagaagtaa gacaaggaac
    11101 gattactcta aacgttccga cccgaacttt tagagaacaa gtttatgaag gcaacgttat
    11161 tcacaataaa aatacgattc tcacccatgc agtgaacaac gctattttaa aaacggataa
    11221 caacggcatt cagattaata aatcaaagaa cagtaacaaa attgatccaa tagctgcatt
    11281 aatcaatgcc tatgtgtttg caatggatta cttcaccaca acggaaggag cgaaagcaga
    11341 caatgaattt tatacaagtg aagaattttc tttctaatta cattcatacc gttcttttac
    11401 ttcttggatt ggtgtgtgtg ttggttgcta tcaccttact aacaaatgtc tactatggct
    11461 tgttagcgct gggcatagtg cttattgggg tagcggtcat gctaaataca gaacagaagg
    11521 gaggttaaaa gatggcattt tttaaagcga gacaaaatac aacgggagat cctttcctac
    11581 aacatgtggt atcaatccaa tcggatgatt acaccaccag ctttacaagc gttcgtgcat
    11641 taagaaatag tgatgtgttt gcagccgttc gaattattgc tagtgatatt gcttcaagtc
    11701 cgattcaatt ggtcaaaaac aatatgccac aagctgataa tgaactggtg aagttactaa
    11761 acgagaaacc taattcagaa atggatggtt ggcatttaaa gtttgctttg gcggtcaata
    11821 tgttgctaaa cggtaatagc tttgcagaga ttaaacgcaa cggtgaaaag gtagaagaaa
    11881 ttcacttatt acctaactca agtgtaacgg ttactcaatt agataatggt acgttgtctt
    11941 atcagattgg tgataaaaaa agacgtgtga agtctagcga tattttgcac ttcaaatatt
    12001 tcacccaaga tggtttaaca ggattaccac cactttatgc gttacgtgat gaactgaaaa
    12061 ttcaacaagc tggcaaccgc acattgcaca atttctttac tcgtggtgtc agtgggtcag
    12121 gtattttgaa ggttcataag tcagatttag acggatcagc aaagaacgca attcgtgaaa
    12181 agtttgaaga agccaacggt tcaagtagtg gagataatgc tcttagaacg atcattcttg
    12241 atgaaacaat ggactacaaa acattagaaa taaacaccga tgttttgaag cttatcaatt
    12301 ccaatgattg gaacacaaaa caaattgcca aggcgtttgg tgtaccgatt gagcgtttag
    12361 gcgttgaaaa tgaacattct agcaccgttc aaagtaatct ccagtatatc caaagtacgc
    12421 tgattcatta ctttaatgtg tttgtgagtg aattagatac gaaacttgaa accaatatcc
    12481 gctttaattc cgatcagtta ctagagactg atcctgaaac aaaagtaaaa aatgtattgg
    12541 atcaggtcaa agggtcactt ctcacgatta atgaggggcg gtcgaaaatg gggctacccc
    12601 ccatggatgg tggcgatcgt ttactagcaa gtttgaactt tacgtattta gatacgttgg
    12661 agaaatatca attaaaagaa caggaaggag ttacaccagt tgaataatga agaagaaaag
    12721 gaaaaacggc tgacagaaga agctgagcta acagccgatt ctcccaaagt agagaaagaa
    12781 aatgaaaaac aaccaacaga cggcaaaact atttcaggct atgcgttgaa attcgggcaa
    12841 ccgtcaaaag atttaggcgg ctttgtagaa gtgattacac ccgaagcatt aaaagaggtg
    12901 gatttatcaa atgtgttctt attatatgga cacgattatt caaagccact agctagtgtc
    12961 aaagcaggta cgttgaaatt gaatgtcgat gatacaggat tacattttga agcagagcta
    13021 accgacacga cctattcaaa tgatgtgtat gagaatatct caaaaggggt tgttgattct
    13081 atgtcctttg ggttcgtgct tggattagat tcattcgaca aaaaagaaga tggcacaatt
    13141 gaacgatcaa tagataaaat caaagcactt aatgaaatta gtgtcgtgac cgttcccgct
    13201 tatgattcat caaatgtcca agtaaataag cgttcttacg aatcgtttat gagtaacaac
    13261 caagcaaaac aaacaaacaa tagcttagaa tccacttcta aagcacaaaa ggagagtaat
    13321 aacatggaaa aaactttaat cgataacgaa aaaacagaat tgcgtgggta tgaagaatat
    13381 atccgttcac aaggcgaagt gcgtgatgga gtcactactg taaatgcagc ggcagttgtt
    13441 cccgaagaag taatcggtga agtctttgat ttgaaacgtt caaattataa cttagctcaa
    13501 tatgcaacag taaaaacagt atcaaatgga caaggtaaat atccagtagc aactaaccaa
    13561 caagcagtgt tagcaacaaa agctgaactt gctgaaattg gtgatattga cgctgaaatg
    13621 tttacttcag ttgattataa agtagaaact cgtgctggta agattgcctt atcaaatgag
    13681 gttgtggaag attcagcagt gaatattgta caagaggtca aagatcagtt agcgaaattg
    13741 gtagaaaaca ctgacaataa gcatatcatg gatttattaa aaacattcac taagaaaacg
    13801 gctgctacgt tggacgattt gaaacaacta tacaatgtgg cattagaccc agcattaaat
    13861 aaaatggtaa ttctaaacca aagcggatat taccacctag atacattgaa agattcagat
    13921 ggacgttaca ttttacaacc cgatgtgaca gcacctagtg gtaaatcatt attcggtatg
    13981 ccagtagtat tgattgcaga tacattgttt gccaatccta aagctggaac attccctatg
    14041 attatggggg atattgcaca atctatcttt gttgctcgta gaaatcaagt aacgactcaa
    14101 tgggaaaaat tcgattacta ctcacaagga cttgcagtga tcgttcgcaa cgattataag
    14161 aaaattgatc aaaatgcttc agtgtatatt gagtttacgc cagttgtaac accaaaagta
    14221 tagaaaaata ttgggcggcg gtttatccca ctgccttttt ttcattagga gagataaaat
    14281 ggtaattttg gatagcataa aaaaaagtat gcggatcgat cacactattg atgatgactt
    14341 tattcaacaa ttgattgata cagcaggcga atatataaaa agtgctattg atagtagcgc
    14401 aacggataaa gatatggata attatcagca atttgatttg gcggtgtcat tacttactca
    14461 acattggtat ttgaaccgtc aagaagccag cagcgaacgg ataccagtaa cggtacaagc
    14521 attagtacaa caaatgagag gtgcttatta tgccaatcat taagaatgta aatgaattga
    14581 ctgagagagt caagtttaaa aagacaaaac gggtaaaaga tgaagatggg cagatggtgg
    14641 atagtgaaga aactgtattt gaatgttggg ctaatgtgcg ttcacaaatg ctaaaagacg
    14701 tgcttgctag tgtaggtacc attcttgaag gaacgttgac gtttattatt cgatacgatc
    14761 aagattatga actaacaaac gatatgaaag tagcttggaa aaacaagagt tataaaatta
    14821 tttcaatcaa tgaaggaacg gcgtttaaag attatacaac gatcatagct aaacagattt
    14881 cttagaatga ttacacttgt aaacgttgtg ggtatttagt ataattaagg tagtaaatga
    14941 agggttagct gcctgaattt tgcaagactt agctagtcga aatctattgt tggactgaaa
    15001 attgtaggtg tggttgcaaa catgctggac taagaaagat gatagatgat gaacgttcaa
    15061 ttttaataca ggcatgctag gactgattgg atggttttgg caagctgatt gtgtagaaat
    15121 caaagaacac gtctgttgtg gacgtgtttt ttttgttata attcaaaaga ggtgatttaa
    15181 tggacaaagc agagtttaca aatttttatt ggaagtatta tctacattta gaaaatgagt
    15241 ttattaatac cacagatttt gtgatgctcg atgagaataa tttcaaaaca ttctctattg
    15301 aatatcaaaa actactacta gcgattggtt cggagtgtga gattattttc aaagaacttt
    15361 gcggatttga tagcagtagc aataaaagca taactgattt taaagctgtt attaaatcta
    15421 gcgaattaat gtgtttggat aatggtgtta gggttatgaa ttctttaaca ttgacttcac
    15481 taaaaccttt tggagatggg tggcctgaag atacaccaga ttggtggaaa atatataaca
    15541 gagtaaaaca tggtcgttct tcaaattata aggaagcaaa tttggaaaat gtgttgtatg
    15601 ctttagcaag tctatattta ttagaggaat atttgcataa gaaagtaata ctagaaggtg
    15661 aagtagactt tataagtcca gagtcaaatc tatttacgtt atcttgggaa agaaaacatt
    15721 cgtcgatgca aaaagtgacg tttgaaaaag tggattcaaa ttatgtagct ccactcattg
    15781 attttaaaga acaagaagaa atttaaaaac ccccacggct tattgaagtt tttgtatgcc
    15841 atattgtatg ccatttggtt taaatcgctt gaaaaatagt gatagataac tctaattcat
    15901 ttagtcataa acgctgtttt gataaccttt atagttcttt gatatgggtt tctcattcat
    15961 ggcggaagaa gaagaataga acaaaagaat gaattaataa gatataaaaa aagggattgt
    16021 gacaaacgtc ttgtcacaat ccctttttcc aaataaacga tgttcaatca gctgcccctc
    16081 ggcattcgct cgaaaaacgg cgaaatatga agaactattt tgccatttgt ttctctaata
    16141 atcaggtcaa aacgttgact tcacagtctc ttccttaaac ggtgttctcc tgccaactcc
    16201 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa ttgttccttg
    16261 tgactcggag ctgaacggca ggctcacgac ctctttctta aacggtgttc aaaagcttgc
    16321 atctgcggta ataagttcaa ccaaacgaaa attatgagga gctatttttg tttggctgtt
    16381 cttattactt gatgcaggac agctttttca caacctcttt tcgtttagca ctaaatttcc
    16441 ttaaaaagta gaaaagcttt cgaagcctcg tttgtttctt ttactgtata ataaaagaaa
    16501 agagtaacaa tcgctaaaag atataaacaa gagaaaggaa atacaagatg attggtctga
    16561 tttattttgt ggtgatcgtt ctggcgaaca cagttggcgc tatttccggg atgggtggag
    16621 gcgtgttgat caaaccgatt cttgatctta tcggcgccca ttctgttgca gggatttctt
    16681 tttactcaac agttgcggta ttcacgatgt ccattgtttc gactgtgcga caagtatcaa
    16741 gcgggaaatc actgaattgg cagattgtcg gttgggtatc tagtggagct gttgttggag
    16801 gagtagtcgg caacattgta ttcgaagttt ttttacagtt atttgaaaac gagaagcatg
    16861 tgcagctgat ccaaattttt ttgacggtat tgaccttggt ttttgcgttc ttctacacca
    16921 agcatcatca gccaaaattt catttgacgt catggacttg gtaccttata tgtggggggg
    16981 ttctgggctt tttagctagt tttttaggta ttggtggtgg accagtcaat gtttctttgt
    17041 tgatgctgat gtttgcattg ccaataaaag aagcaacact atattctcta tctacgattt
    17101 tcttttctca actggcaaaa ttggtaacga ttgctttaac aagtagcttt atgcgctttg
    17161 atttatctat gttgttttat gttatccccg ctgctgttat tggaggtttg tggggagcaa
    17221 gatttagcag ggttttgtct ccaaaaaaag tgacctttat ttttcaagta attgtcctcg
    17281 tggttctttt gatcaatctt tacaatgctt tcgtcatctt acaaacatta ggagaatcta
    17341 gactaacgca agtcaaaatc actgaaacat attgttctaa atgaaaaaag gagtatactg
    17401 tgataaaaaa attcatttaa aaaaataaaa attggctggt gatttggatg ttgcgggaaa
    17461 tgcgtttagg agatgcagaa gcactacgag aaatcaatgc ggaacaatta ggatatgatg
    17521 taccattaac cgttacggtg agacaaatga agaaactgtt gaaggagcca gaaaaaaatt
    17581 ttttcttggt ctatgaagat cctacttcta aaaaagcagc tggctatgta caagcggagt
    17641 cctatgaatc gattttcagc gatccgatgt tcaatattat ggcacttgct gtgtctaaac
    17701 atgctgaaaa aagaggaatc ggaaaggcct tgatgactgg cgtagaagaa gcgaaaagac
    17761 gaggaatatc agcaataaga cttaattctg cagaatacag agtagaagca caccgatttt
    17821 atgaacgcat cggttatcat tccgataaaa tgcaaaaaag atttttgaaa atcctgtaaa
    17881 tttttatcaa aaaatacctg gtcatcaagc cgatacttga tgatcaggta ttttttttga
    17941 ctaagaaagt aattgcatca agaaacctga gatgatcaaa acgatggcac caccaagccg
    18001 attgcccatt tgagcaaaag caataagttc catccgatta gctgcagata agacagctac
    18061 atttcctgta ccacccatcg agttgttgca taagccagca gtaatagttg attcgattgg
    18121 atataaacca aacaaacgac cgacaaatcc tgaaactact gaaatcgcga taacacttgt
    18181 caaacataag acaacaaatt gccatgtcaa agcagaagcc aatacattta gattcaataa
    18241 cgcaataccg ataccggcaa gaacagctgc tgtcaagttt ttgacaatca agttgttgaa
    18301 catgatagct gcatcttcgt aatattctgg taataggttt gcgattttac agataacaac
    18361 gataatgatc atgaatgcgt aagcatggac tttaggaaca aaatagttac agatttgtcc
    18421 taagatgaag aaagtcaatg ataccatcaa accgacacct aattgtggaa tggaaggttt
    18481 tacatttcta ggttttccat cgccaaggtc gcctttttcc attaatttac cattaccgtt
    18541 gaattttggc attgattgtc ccgcacgagc aactaacgct gcaccaatga tagctaggac
    18601 atttgtcatt gctgtcgcag gaatcaggcg ggaaatgata gaagaagcgt ccgtacctaa
    18661 aacatttgca taaatcgttg ataaaggtac agctccggct ccaacaccac cagccattgc
    18721 gggcaatgag atatataaaa cggaatttgc aaaaccattt ccgataagca tacctacgag
    18781 tcctacggca aaaaaggaga cgaccattga aataagtgca acgggaataa aacgaacaga
    18841 agctttcatc aatagattac ggttcatacc aaggatgcta ccagtaatca aggcagcaat
    18901 ataaaagtct aaaaagccca tattgctgac aaagttttct gtggatttta ctacatactc
    18961 aggtaagatc ccaaatgtag caatcgcggc agaggcaaag atcgcaaaaa cggagccacc
    19021 acctaaataa cttttgacga tagggatttt tgttccgata aaatgaaata aattaccgaa
    19081 aatgaccaaa acagcgattg ggccaatcat attcgtcggt aattttccta aagccattgc
    19141 aatgatcagg acaaggacca tgatcaaata aaggggcaaa cttacgccgg taatttttgt
    19201 tgaccaaaac ccttcttttt tcttttcctc aactgcagtt gcggttgttg ttttttgtgc
    19261 catttttaca gatcctttct tattctgtat cgtatgaaaa aagccaaaga gaagcggcaa
    19321 aagagcagta gttggctggc tgctgttttg ccgcagtcgc ttttttcttt ttagacgtca
    19381 tacaaagttt ctgtatgttc ttttactata acaatcttaa tcttctaaag atttatattc
    19441 aggaacccat ttagcagatt caacagcttc tttgatatcc gtgatttctt cacgattcaa
    19501 tttttgtgca acaactgatt ttgcgacagt ttcagcaatc gtttgagaga attcagtgat
    19561 ttttgctact ggaggcaaga tagctgctcc tggttttgtt acatcaacga ttccgcctaa
    19621 tgctcgacta gcttgagaaa tgatttcagc atttactcgt gtagctgttg aagcaatcaa
    19681 accaagccct aaacctgggt acatcaacgc attatttgct tgaccgattt ggtaagttac
    19741 accgttatat tccacatcag cagcagggat acctgttcca actaaagctt ttccgtccgt
    19801 ccattcaatc aaatcttttg cttttgcttc tgccaatttt gttggattag acaaaggcat
    19861 gatgattgga cgtggggtat gagccgccat ttctttcacg atttcttcag tgaatgctcc
    19921 aggttgggta gaagtaccga tcatgatcgt tggatggatt gcttttacaa ctgcttctaa
    19981 gtttgtcaat tcatcagcat tagtgaattc tgagcgttta cgagcgaatg ggatttgacc
    20041 aggagtcaat ccttttgtat cttcaaacaa taaaccctgt ttatctactt gatagaaatg
    20101 ttggcgtgct tcttcttcag gtacaccttg acggatcatt tcatctaaaa ggatgtttgc
    20161 gatacctaca ccagcagtac cagcaccaaa tgtcaagatc gtttgatctt tcaattgctc
    20221 acctgatata ttcaatccac ctaatacacc agctagtaca acgattcctg tgccttggat
    20281 atcatcattg aaagtagtga ttttatcttc gtatttttct aagatcgttg cagcatttcc
    20341 tcgtccaaaa tcttcccaat gtaataacaa ttctgggaat aattcagtag ctgattgaac
    20401 gaattgatca atgaattcgt agtaagtttc gccttctaca cgagcatgac gattccctaa
    20461 gtataatgga ttattcaata attcttcgtt attagtacca gcgtcgattg atacaggcaa
    20521 gacttgtgca ggattgatac cagccgcagc agtgtagacc atcaatttac cgatagcgat
    20581 gtcaacaccg ttcacacccc agtcacccat acctaagatt ccttctgcat ctgtcacaac
    20641 gatcaaacga atgtcgcgtc catctgctgc gtttttcaaa cttgctttga tattttctgg
    20701 ttcgtcgatt gaaagaaatg cagcatcttg tggtttcaag aaaatttcgt tgtattgttc
    20761 gattgaatcc gcaacgacag gatcataaac gacaggcatg aattcaacta aatgttcgcc
    20821 catcaattta taaaacaatg tccggttcgt attgaataga ttcattaaga agatacgttt
    20881 ttccaaatcg gtctgtttgc ttaagtattg accgtaagct tggacagatt gttgttccaa
    20941 tgtttggaca gtacttggaa gcataccagt gattccgtat tttgcccgtt cttcttttgt
    21001 aaaagctgtt cctttattta gaaaaggatt atttaataag tttagccctg taagcatgaa
    21061 tgattcctcc taagttttaa tacaaacgaa gtataggagt cagatgagga tatagtcaaa
    21121 aagagcaggt ataataaatt ttatttatat gagtaataga gtcaaaagaa atcaaaatag
    21181 cggtatttgt tctttttata tcaacaatcc ttgcttgatt actatgcgta tctcttatat
    21241 gctaaagaaa gaaaagtaga agaataaagg atatgttaga tagataaata gaatctctga
    21301 agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata aaaaatacat
    21361 aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc agaaaaagcg
    21421 aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc tatacattaa
    21481 agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt gtggacagtc
    21541 tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca gaaatccgtc
    21601 agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat gtgagattca
    21661 aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc gagctatatg
    21721 gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg ttcaaggaag
    21781 aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata caaagaaaca
    21841 acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt gacacgactt
    21901 ttgtcaaagt cccatcgttt tttatctatt tgtgttctaa acatttcttt cagagtagta
    21961 acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg gaagagtaat
    22021 ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg aaggcattga
    22081 aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta tcaatatgga
    22141 agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt acatcagcag
    22201 tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc atcagctgat
    22261 tattcctcat gtattaaaag atgtagaatt acccgataca actacacatt ttgacaaaga
    22321 aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag ctcatgtgaa
    22381 ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata cagcaagttc
    22441 ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag caccgcaatg
    22501 gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg aagtggccaa
    22561 gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg gaaaccgtga
    22621 aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc aggcttatca
    22681 atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga agcttagtcc
    22741 aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga aaggtgtcca
    22801 gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct gggtatctaa
    22861 tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc aatatgtggc
    22921 agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac gcggacagca
    22981 tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac ccgttattta
    23041 tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct ttaaaacaga
    23101 attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca aaaaaataaa
    23161 attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga gtgcggcaga
    23221 tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa tttgaaacga
    23281 aaaaataaaa ttgaaatatt ttttcatact taataaaaaa ggaagaatat tcatgtaaac
    23341 gattttattg tttttttctc ttttatacaa aaagggtcat aatttcaagt aagcggatga
    23401 attcatgagg attttttgca tactgtctct actatactta ggtcactaag aaaaaggagt
    23461 gatttattat gaaaaagaac atgattggga cattattact ttcaacactt cttctaggcg
    23521 gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat attaccttta
    23581 ctgaaccaac aaataccgtg gagcctctta accctactga tccttcaaaa ccagtagagc
    23641 cagcagatcc ggaaaatcca gcaactggcc agacaggtcc tttaacactg gatgtagttc
    23701 ccgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat caagtagacg
    23761 cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta ggtgcagatg
    23821 gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac ggttcacaag
    23881 ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg tcagataacg
    23941 aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt gcagcgacac
    24001 caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt tatgatccag
    24061 caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct gatttgactt
    24121 ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa actgcggtga
    24181 aaatactata ggtgaaggat tttttaccgc agtttttgtt gaaaggatgt ggtggacaat
    24241 gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat ttttcatttt
    24301 tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac cggtatttcc
    24361 agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac cggaacaaaa
    24421 tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc
    24481 tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac
    24541 gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat
    24601 caatgtatca gcacatgcag aaacaactgt acctatagaa gtgaaaatcc cagcaaaaag
    24661 ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag ctgaaacaga
    24721 aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt
    24781 acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag atttggacga
    24841 ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta ggattatcag
    24901 caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg aaaatacgtc
    24961 taatgcattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt tagctggtga
    25021 taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg acagtaatga
    25081 atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa
    25141 ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat tggttgctgc
    25201 agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagg
    25261 agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga gggattattg
    25321 caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa agttcaggta
    25381 cgattacatt ttatgatcta agtccaattc cgcatccaag tggttcagtc aatggcgcat
    25441 caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc gtattcccgg
    25501 agacgaatga acaaaagatc cgttttcttt atttgatcat tggtatagaa ttatggcttc
    25561 ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt gctacaaaat
    25621 aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag tatatttatc
    25681 ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta tgatccatac
    25741 acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt
    25801 gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt gaacgatggt
    25861 tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca tgtgaaacta
    25921 acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt ggcgatcatc
    25981 aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg gaacaacgcc
    26041 tacttgtata ttgccggtgc ccccatcaag gttactagcg gaagtttatt atcagcagga
    26101 agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt gacagatatt
    26161 attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca atttgatgtt
    26221 acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag tccaaacaaa
    26281 attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt ttctgttgat
    26341 ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt agctccagct
    26401 gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc atatgcattt
    26461 tctgcaagtg cagagataaa tacgtggaat agttacatct caccaagtga ttcaacagga
    26521 aaatataata catttgctga taccacagaa tacgtggatg gaaataacca gaaccttcca
    26581 ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt tgatcagttg
    26641 ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt tacacaagga
    26701 gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc caatatcgtg
    26761 tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa ggtattggag
    26821 cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag tcagtgggga
    26881 aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac tgaactaaaa
    26941 aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc gacaggatgg
    27001 acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac agctacttct
    27061 acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt ggacgatgta
    27121 cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc tggaatcacc
    27181 actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag tcagctaaaa
    27241 aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg agatacgatt
    27301 caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga caaaaaaatc
    27361 acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt aaatggtaca
    27421 actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct attgagcaaa
    27481 ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga tacttcttta
    27541 caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac attagcagat
    27601 ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct tgtccccaaa
    27661 caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga tctccccaaa
    27721 ccgttgaaga caagttatta tgaatcaaaa ggcgattttt cttttgatgt gcgggatact
    27781 cggcttccaa gcacgtctcc ttggcaactg acaggtacat tgacttcttt atttaagaat
    27841 gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg atcaaagcaa
    27901 ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc taaaggagag
    27961 gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag cagtacgaat
    28021 gctcaacctg gcgcaacgta tcaaggcatg gtcacatgga aactgacagc tgggccaacc
    28081 agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct ctttttcaag
    28141 gatgagaagg aaacttctgc tgttaaaatc agaaatgctt gaaggagtgg acttggaact
    28201 tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac ttttattaga
    28261 ggaaaaagga gaggtttccg ttaataacgt acttacccgc ttatcagttg atcgggctac
    28321 cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac aattggaaat
    28381 tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag cagtcgatgt
    28441 ttattatcat tgttacttgc gctattctac aaaatatcag atacttcttt atctgttgga
    28501 aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt cggctacttt
    28561 gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga aaataaaaag
    28621 cgggcggctg accggtccag aaagtcaaat tcgttatttt tatttccaat tattttggtt
    28681 tggacgtccc tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa acactttttt
    28741 agatctgctg caaaaagagt ttagttttgc tattacagaa gaaggacgat tgaaatttga
    28801 aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca aaccagatta
    28861 tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc tccatacatt
    28921 tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa cgttggtttt
    28981 ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg tccaggagtt
    29041 tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa cgtttaaaca
    29101 atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc aaaagattgc
    29161 gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga tgaatatttt
    29221 tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat tcctggtttt
    29281 atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag tagacgagga
    29341 aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttacggacgc tgatcaagca
    29401 gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa tggcggatat
    29461 cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt tagtcgaata
    29521 cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt acccgtcaca
    29581 gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt tcccctattt
    29641 gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga ataaaacatg
    29701 aaaatcgtga tcattggtgc ttcccatgca ggcatcacag ctgccctaaa tctaagaaaa
    29761 cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg actaggctat
    29821 gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc tgaagttgcc
    29881 cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg gagagtaact
    29941 gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa aaacgagacc
    30001 ataacctatg ataaattaat cgtagcgaca ggttcctctc ctgtcacctt gtataaacaa
    30061 attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca agtacttgcc
    30121 gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttacattgg attggaatta
    30181 gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat gccgaatgtt
    30241 ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct gcaaactaag
    30301 caaataaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga agagaaagta
    30361 gtatccgtac aacttctgtc tcaaacaata aaggcagata tggtcatttt ttctgcccaa
    30421 actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga tgagactgtg
    30481 atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg agatatcgtc
    30541 ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac tcgagctgta
    30601 catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta cgacctcaga
    30661 caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg gctgacagaa
    30721 gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt tgatctattt
    30781 ccacaatacg acgaagaaaa caaaacagta aatgcaaagc tgatctacca tccggataca
    30841 ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga tttgaacctg
    30901 cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga ggattttgga
    30961 tttctgtctg aatacacctc tcgttttcac tacctcaatg agttagcttt taaagtacta
    31021 gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc acggtctttt
    31081 attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat ataatgaaat
    31141 agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat aaaattgttg
    31201 tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat gaaattatgc
    31261 attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc tacctggtat
    31321 tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca gtcgacaagt
    31381 gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg atgtccatga
    31441 ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt gcccaaacac
    31501 ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt agcttcttcg
    31561 gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct tttgataacg
    31621 atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc ataggagata
    31681 cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg gatcatgcca
    31741 aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagaa atccgcttcg
    31801 aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga ggtaaaatca
    31861 gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat gaattacctg
    31921 gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc gttattgcgg
    31981 ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat atctttggtg
    32041 taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg gcacaaacat
    32101 tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga acatcccttg
    32161 ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc attagcttgt
    32221 tgatgttagt agtagctgtg ttaggcgcac tattttctgt tcggacaatc gtaaaaatcg
    32281 atccgttgaa ggcaattgga taagaaggag gaagaacatg aaagcaatag aatttaaaga
    32341 agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag aaacaaccat
    32401 gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag gtaaaagtac
    32461 ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat ttatcaatga
    32521 tcaagaattc agtaatcaaa aagaaagcga acgcgttaaa ctacgtttta aagaaattgg
    32581 ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc agttattatt
    32641 agtagataag attaaacatg aacatcgtca aaaagaagca aacgaattat ttgagcagct
    32701 tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag aacggcaaag
    32761 ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag atgaaccaac
    32821 tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa aagaatcaaa
    32881 agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg acaaatgcga
    32941 tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa aaagcaggtg
    33001 gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa gaagcgactg
    33061 acttgtttat gtccaaggga tatctcggca cttcaacaag agaaatcgca caaaaagttg
    33121 ggatcaccca gcccaatctc tatcattatt tcggtgataa agaaaaacta tatacggcag
    33181 ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt caaacaagcg
    33241 aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag acacatctgg
    33301 tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa acaagagatc
    33361 atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc ttttcagcaa
    33421 accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat tttttcttag
    33481 tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta aatgtggcgc
    33541 aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt tatataggga
    33601 gaaaatagtt aggtgaagaa gcaagatatg acgaagatcg ttatatcttg cttctttata
    33661 taggtagttt ttcataaaga caaatttgtt attctctcat gattatcagg gtatattttt
    33721 atttattatg tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt
    33781 gtagaatgaa ggaaactatt tattgggtag atgaatcacc acgaatagtc aaaaggagat
    33841 ttcaggatat gccaaaaatg aaaaaaatca ttttagcagt aatgttattt tctacggtta
    33901 tatcttttac agcccctttt ttgcttactt aagctgattt taagttttgg cccttagtct
    33961 ggatctacga ggtgagagtc aagaatgaaa aaaataattg gagcttttgt atttgcaatc
    34021 attatcatta ttatctgttt tcaaatagat gtttttggtc aaaaaaagat agttggacaa
    34081 acggtcagca gtacgaacag tacggttaca aagacaaagg aatcatttga tgatacagaa
    34141 caagagacat taatcaaaat cggaaaaaat gaccgtacac tatatacaga tgactcgcta
    34201 actaccccac ttgcaactat aaacggcgga gagctggcgg agtttctttc tgaaacaaaa
    34261 aatgcttatc agatcaagac aaatgatggc tatactggat atttagccct cgaagatgga
    34321 accaaagtca caaagaatat acaaacaaaa ccggaagagc tatctgatgc agtgattgta
    34381 ttagatcctg ggcatggcgg aaatgatact ggggcattaa gcaatgatga acaaacagaa
    34441 gaaaaagata taacattaag tacagccata aaagtaaaaa aggcgctaga ggatgctgga
    34501 gcaacggttt atttgaccca tactacagat gaattagtac agcttggaga tatatgcgac
    34561 tacagtgaag aaaaaaaggc agatgtcttt atcagtttac acgcagactc tacggaatat
    34621 gcgaatgaag caactggaat cacgacatat tattactatg gacaagagga aacattagca
    34681 cagacaattg ctgactcctt cactgatctg cctctggatt caagaggaat ctccactggg
    34741 aactatcaag ttcttcgaga aaatcttcaa ccttctattt taatcgagat gggttatatg
    34801 aataatgata gtgatttaaa ggaattagta acagacagtt atcagcagca gattgctgca
    34861 agtttgacac aagcattgac cacttatttt cagtaataaa taagcccagc caaacaaaaa
    34921 tagctcctca tatttttcgt ttggccgggc ttattatcgc agatgcaagc ttttgaacac
    34981 cgtttatttt tttagtagct tcaaagcttt ttctgtattt gcaaagtgaa gtttggcata
    35041 tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt ggtcagattt
    35101 cgttccagct cctgatggaa gggtgattcc agcttcagct gatgctttgt gtaactcttc
    35161 caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat attgttctcc
    35221 tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg catatttttt
    35281 aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt ctggctcgat
    35341 tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt
    35401 gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt tataggggat
    35461 ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact cttttgaatc
    35521 tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag cagcacatac
    35581 tgttagcgga ccgaaatagc tgccatttcc gacctcatca ctacctaaaa cggataattg
    35641 actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc
    35701 ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca cttttccgga
    35761 tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat aaggaatctg
    35821 tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt cgtttgttgc
    35881 tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac tataccgcaa
    35941 ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa agtttatcat
    36001 ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca acttcatggt
    36061 acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca cgaaaaaaca
    36121 cgctacaaag cggtgattgc caaccaaaca tatactatta tcggacggga aaccaagcat
    36181 catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa acaattatca
    36241 ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa
    36301 ttgaagaaac aagaaagaat cttggagctg gaagaagaaa cagcagaatt aaagaaaaaa
    36361 atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa
    36421 gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa
    36481 gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa
    36541 gatcctttta tcaaaataag aaaaatttaa aaaaagttag aagagaaagg aacgtagcat
    36601 gctgagtttg ctgattttat ttttattgct aattgcgttt ttttctggag cacgtcgcgg
    36661 gtttgctttg caaatcatat tcgcgattgg atatgtattg tcatttattg ctgcccagca
    36721 tttttacaaa ccattagcaa gtcatttgga attatacatt ccatacccag ctgtcacgcc
    36781 aacatcaaag ttagcatttt ttgatcaggt atttgccttt cacctagatg aagcttttta
    36841 tgcaggaact gcttttctga ttattttgct aattggctgg ctgcttaccc gatttgtggg
    36901 ggtttttgtc catggattga cgtatgtgcc gatcttgaga caggtagact ggattgcagg
    36961 aggcatcctg tcactaatca tggcttacgt tactatattt ttgattttgc agttacttgc
    37021 ctttgttcca ttagatgtgg tccaaaatca attcggcgag catagtttcg ctcgctttat
    37081 cgttgagaga acgccatttc ttaccaacaa gatgtatgag ctttgggtaa cgaatatttt
    37141 gaattaagga aaatagtgct ggaaaatatt tcgttttttg tacagagtag attatttaag
    37201 aaggctgcgt agcgtatgtc cggcacagct cttttttcat agtgaaagat aaggtgataa
    37261 aatgaataac cgaatccttg aaacattgga atttgaaaaa gtcaaacaga tggtcagaca
    37321 atttgtagtg actgcacaag gaaaagaaga actggcagaa ttggtaccag tgagcgagga
    37381 acaaacgatc acaaactggc ttcaagaaac tgaggatgga ttgaaagtgc aacgtctgcg
    37441 aggcgggatc cctataccta aacttgaaaa tatccgtccc catatgaaac ggattgagat
    37501 cggagcagac ttgaatggag tggagcttgc acaagtttcc agagtattgt ctacgacaag
    37561 cgaattgaat cgtttcattg atgatcttta tgacagtgaa atcgaatttg cacgtttgta
    37621 tatgtgggca gatcagttag tcacgatccc tgttttgagc cgccgtttaa aggaagcaat
    37681 cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag ttaaagagca taagacagaa
    37741 cattcggcga agtgaacaag ctgttcgtga acagttagat gggatcgtcc gtgggaaaaa
    37801 tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat gaccgctacg ttatcccagt
    37861 caaacaagaa taccgcggtg tatttggcgg tgtagtccac gatcaaagtg cttcaggaca
    37921 gactttattt attgaaccaa aacaagtggt ggatttgaat aatcgtttgc gtcaatacca
    37981 aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa ctttctgctg aacttgttcc
    38041 ccatcgtcaa gaaatcatcc acaatgctta tgtgattggt aaaatggatc tgatgaatgc
    38101 aaaagcacga tttggtaaag aagtaaaagc tatcgtgccg ggaatcagtg aagacaatca
    38161 tgtcgtcttg aaacaagcaa gacatccact gatcgatcag gaaaaagtag tttctaatga
    38221 catcacaatc ggaaaagact atcaagccat cgtcattaca ggacctaaca caggtgggaa
    38281 aacgatcacg ttgaagactt taggtctgtt acaactgatg gggcaagcag gattaccgat
    38341 tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa gtatttgcgg atataggaga
    38401 cgaacagtcg attgagcaaa gtctatcgac cttttcttct catatgacga acactgtaga
    38461 tattttatca aaagtcaatg aaaaaagtct ggtattattt gacgagctcg gtgcaggaac
    38521 agatccgcaa gaaggtgctg ctctagcaat cgcaatctta gatgatcttg gtaaaaagtc
    38581 tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa gtttatggat ataatcgggc
    38641 aaatacgatt aatgccagca tggagtttga tgtggataca ttaagtccaa cgtatcgttt
    38701 gctgattggc gttcctggac gaagcaatgc gtttgaaatc tctagccgat taggtcttga
    38761 tacagaagtt attgatgaag cgaagcagtt gatgaatgat gagagccaag atttaaacga
    38821 aatgatcaca gatttagaga atcgccggaa aatggcagag accgaatatc tggaaatgcg
    38881 tcattttgtt tctgaggcac aagaacttca cgatgatttg aaagaagctt acagctattt
    38941 ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa aaagcaaatg aagtcgtgtc
    39001 agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt cgtaaaatgc aacagcagat
    39061 tgggcaagga aatgtgaaag agcatcagtt gatcgatgca aagacccaat tagcaaatct
    39121 acatcaagaa gaaactttga agaaaaataa agtattgaag aaggcgaaag aacaaaaaac
    39181 gttgaaaccg ggagacgaag tgttggttac gacttacggc caaagaggga cgcttttacg
    39241 taaaaacggg aatcagtggc aagtagaaat cggtatatta aaaatgaatg tgtccgaaga
    39301 cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa cgagttatcc atgctgtacg
    39361 atctgaatct agtagtcacg tgccgaatca attggacctt cgaggaaaac gatacgaaga
    39421 agcgctaagt gaggtagatc aatatttgga ttctgccatt ctagctggtt atcctcaagt
    39481 gactattgtc cacggaaaag gaacaggcgc acttcgcaag ggaattactg attatcttaa
    39541 aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca aaccaaggcg gaaatggtgc
    39601 gacaattgtt aaatttaagt aaagcttact aaaggtaagc agatggttcg ctatccaagt
    39661 atagaaatgc tatactagtc atagatttta aaaatggagg tctttgatat gtcacaagta
    39721 attacagata agacatttaa tgaagaaaca gataaaggac ttgtattgat tgatttttgg
    39781 gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt tggatcagtt ggaacaagaa
    39841 tatgatgaag aagaattcag aattgctaaa atggatgtgg atgaaaatcc agaaactcct
    39901 caacaattcg gtatcatgag cattccaaca ttaatgttga aaaaagatgg tcaagtagta
    39961 gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc aaatgatcga tcaatattta
    40021 taaaaactta cagcaaaaat aagctgaggg tgggactgaa tccagttccg tcctcggttt
    40081 ttttgcggtt gagttaaggt tattcaaatt aatatttttt gagtataatt acgttgtcag
    40141 gctggattgt ttttttgaca gacatgctat aataacttta attgaatatg aaggggagta
    40201 ccagatgaac gaacgaatca aaaacaaatt agctttactt cctgatcagc ccggctgtta
    40261 cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc aaagccaaaa tattaaaaaa
    40321 tcgtgtccgc tcatatttta ctggtagcca taatacgaaa acggaacggc ttgttagtga
    40381 aatcgaagac tttgaataca tcgttacaga atcgaatatc gaagcattgc tcttagagat
    40441 caatctgata aagaaaaatg atcctaaata caacattatg ttaaaggatg ataaaaccta
    40501 tcctttcctt aagataacga atgaaaaata tccccgtttg gtgattactc gtaaggtact
    40561 aaaagacaaa gcactgtatt ttggaccata tcccgatgtc ggtgcggcga atgagacaaa
    40621 gaagatcctc gatcgattat ttcctctgag aaaatgcaaa cctagtcaga cgaaggagcc
    40681 ttgtctttac tatcatttgg gacaatgtct gtgtccttat tatttcgacg tagatccgaa
    40741 agtctatacg aaaatcgttg atgaagtgaa aaacttttta aacggaggac acgaaaaaat
    40801 cgagtcagag atccatcaga aaatggaaaa agctgctgaa aacatggagt ttgaaaaagc
    40861 tgcagaatac cgagaccaga tccgtgccat tgagacaatc atgactcgcc aaaaaatgac
    40921 aaatactgat ttactggatc gagatgtatt tggttatgcg attgataaag gatggatgtg
    40981 tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa cgagatgttt caatgtttcc
    41041 tttctataac gaagcagaag aagatttctt gacttacctc ggtcagtttt atcaggaaaa
    41101 tgagcatttc attcctaaag aagtcttgat tcctgatacg atcgacaagc ccagtgtaga
    41161 agctttgtta gctacgaaag tgctgcaacc aaaacgagga gaaaagaaaa aattagttga
    41221 attggcgagt aagaatgcct cagttgcttt gaatgaacgg tttgatctga ttgccagaaa
    41281 acaggaacgg acgatcggag cagttgaacg tttaggaaac gcgatgaata tcccgacccc
    41341 aatacgtatc gagtcttttg ataactctaa tatcatgggg acgaatcctg tggctgcgat
    41401 ggtcgtattc attgatggaa aaccggcaag aaaagaatat cggaaatata aaataaaaac
    41461 agtcaacgga ccggatgatt atgcatcaat gagagaagtc atctatcgca gatattcgcg
    41521 agtgattcgc gaagggttgc ctttacctga tttgatcttg atcgatggag gaaaaggaca
    41581 agtagatgtg gcaaaagatg tcttagaaaa tcaattagga atcgatattc ctattgctgg
    41641 catggcaaaa aacgataaac ataaaacaaa tgagttgcta ttcggtacag atttggctgt
    41701 agtaccgctt gagcgaaatt caccagagtt tttcttactc cagcgaatcc aagatgaagt
    41761 ccatcgattc gcaatcactt tccatcggca gctacgcagt aaaaacagtt ttgcttcccg
    41821 attagatgaa attgaagggt taggaccaaa acgaaaaaaa atgctgctta aagagttcaa
    41881 atcgttgaaa aatatcacag cagccacgac agaagaatta caagaaatcg gactgccgaa
    41941 aaatgtcgca caaaacgtat ttgataaatt acatcaggaa tgaggaaagg gcactttaca
    42001 aagaaagtaa agtgtcctat cattttatgt acaacaaaaa gagcgtttac gttttgttgg
    42061 taaataataa atcgcttacg tctggtgtct aaggatatat gtgatttctt gaagaaggag
    42121 tgaagaaatg ttagaagtca aggatcttgt taaaactttt gggaactaca cagctgtcga
    42181 tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg ctaattggcc aaaacggggc
    42241 tgggaagaca acgacttttc gtttgatctt ggattttttg acacaagacc aaggagaagt
    42301 gttatggaat ggccatcatc ttggggagaa agattatgat gtcatcggtt atcttccgga
    42361 ggaacgggga ctttatccga aagtaacgat tcaagaccaa ctgctttatt ttgcagaact
    42421 tagagggaaa tcaagaaaag agattgaacc aaaaatcgac ttttggatgg aaaaatttca
    42481 ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct aaaggaaatc agcaaaaagt
    42541 ccagctgatc gccacgttaa tccatgaacc taaattgatc attttggatg agccattcag
    42601 cgggttagat ccggtaaatg ctgaattgct aaaagatgga atattggaat tgaaaaaaca
    42661 tggttcatgt gtcatttttt ctagccacaa tatggataat gtagaaaaaa tctgcgatca
    42721 tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga aaagtacatg agattcgtga
    42781 atcatttggc cgtacgaagt tattcttgga atctggttta agccaacagg aagtagaaga
    42841 aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat acgttagaga ttaccttaga
    42901 agatcctgaa gctgggaaag aaatctttac tcgtgcgact cagttcggct acattcctat
    42961 gtttaaccag caaccgccaa ctttggaaga gattttcaaa ttgaaagcag gtgaaatcga
    43021 tgcgtaagtt ttggattatt accaaagatg tctatttaaa aaatgttaag tcgatttctt
    43081 tcttgatcat gatattagtc ccttttatca tgctgggtat catttatgta gcaggaaact
    43141 ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt ggctgataat gagcaaatca
    43201 gtcagcaaat gagccaagtc aaaacagatg attttgagtt caaagcagtt gattctgaag
    43261 aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc gtatttagta gtctcaactg
    43321 agaatgataa gataaatggg atgctttata gcgaaaattc attaggacaa agtacacaac
    43381 tactgataca acaacaactg actggcttgc aatcgatgct tcgagcgagt caattaggca
    43441 tttcaccaga agaagtagct agcttgagcc aacaagcaag tttgactcga caaaaagtca
    43501 attttgattc aaaaggaaaa atgacagtcg gagaagataa ctcgacagtc caatacgtcg
    43561 taagttatgt agcaacgatt gtcttgttca ttattatttt gacgtatgcg cagatcattg
    43621 cacaagagat cgcttctgaa aaaggcacgc ggatcatgga agtgatcctg tccagtacga
    43681 ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt acttgttgca ttaacgcaaa
    43741 tggcattata cggtattatt tttggtatag gatacagtca attcaaagat atggaaatgg
    43801 tcaaaaatat attagacggt atctcattgg ataacttgat tggaccattc ttgtggtatt
    43861 ctcttctatt catgatcttt ggcattttga ttttttctgt tttagcagct ttatgtggct
    43921 ctttggtcaa caaagcagaa gatacagcaa aagcaatttt gccagtgact tatctatctc
    43981 taggcggata catgcttgga ttgatactag gagcttcaga tccaaacaat atcgttattc
    44041 ggatcacttc gtatattcca ttcttatctt catatattat gccggttcgc ttagcaaatg
    44101 agacagtcgg tatgggtgga gcattgattt ccttgttggt acttgtagcc gtaacattct
    44161 tattgatgat cttgtctgca aacatgtaca aatcgaatgt actcgtttat agcgaaggcg
    44221 gattatggag ttcattgaaa caatcgattt caattatgag gaatgaacga aaaaaagcat
    44281 aaaacaatcc taaaatacag aaggaagctg gacatctatt gttcccagct tctttttgta
    44341 tagacctatt ttatttattt tactatattg gatatatatt cattgaaaat agtgtataac
    44401 aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg ttatttagat
    44461 ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat caatctcgaa
    44521 gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca tggcgaattt
    44581 cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa gatcaaagac
    44641 cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt tacggcttat
    44701 ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca gctgatccat
    44761 catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa agttcgaaaa
    44821 ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga tgaccgtatc
    44881 aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat gaaggaagaa
    44941 gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga tcatttgcat
    45001 atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga tcgaatcatg
    45061 gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat tggtgcgtat
    45121 tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta taattattta
    45181 tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt tgctcatcaa
    45241 acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc cgaaagagag
    45301 caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa atcagaacat
    45361 cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct ggaaaaaatc
    45421 gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa aggaacttgt
    45481 tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca aaaagtgatt
    45541 gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat gacgtttttg
    45601 atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca tggctgtata
    45661 cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta tcgcggaaaa
    45721 gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct gactgcaaaa
    45781 gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc gcctttattc
    45841 ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca tacgcacgtc
    45901 gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga aaaaatttta
    45961 gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac gccagtaaaa
    46021 gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat tgacggaggt
    46081 ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt atgcaattct
    46141 tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca atttgcaaaa
    46201 aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg caaactcaat
    46261 cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa aaggctgttg
    46321 gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag actggagact
    46381 gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc tgccaactcc
    46441 tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa ttgctccttg
    46501 tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt caaaagccag
    46561 tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca caaaattttt
    46621 cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta ttaaaattca
    46681 ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt aagttccgga
    46741 tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc ttcaatgagt
    46801 ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac tgcctgatga
    46861 tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact gtctgaaaca
    46921 atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg ttttactgac
    46981 cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac attcaatagt
    47041 tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc caagatcagt
    47101 gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg aggatcttcc
    47161 atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc gatttgtcca
    47221 atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa tccttgagca
    47281 tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc ttggaaagta
    47341 tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat ttgtattccc
    47401 cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta agacttgccc
    47461 ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt gtgaaattca
    47521 tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag aaataaaaag
    47581 gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat tttattgaag
    47641 gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa aagaaagcat
    47701 gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg gaacaaatcc
    47761 gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag ctgaagatct
    47821 atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga caacaactaa
    47881 aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa gaaaaaatga
    47941 aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat caaaagcatc
    48001 agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc cgtcactaag
    48061 atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt acaagcagta
    48121 caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa caaccagtgg
    48181 gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt aagcgcttta
    48241 ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca cggcgatagc
    48301 cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt ggaagccaat
    48361 tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa agaaaagtgg
    48421 gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct tcttcattgg
    48481 caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt tgagatgggg
    48541 actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct tgatgacgtt
    48601 tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt tgctttaaaa
    48661 aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa agaaacgtat
    48721 ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt atccattgtt
    48781 cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta tatccaagaa
    48841 tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat aaaagaacgg
    48901 tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga agaaaaacag
    48961 aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt cagtttcgaa
    49021 acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa ccatccgctt
    49081 gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga agaagtagat
    49141 ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt cgcagatgtt
    49201 ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact tgttctacca
    49261 cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc gatcatctgg
    49321 ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt tggcatccgc
    49381 ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat tttatccgtg
    49441 attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga aaatatcagg
    49501 agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat cctaattggg
    49561 atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt ttaccttggc
    49621 gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat ccaaacgccg
    49681 tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac cggctatatc
    49741 ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg aagtattgga
    49801 gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag tgtagggatc
    49861 cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag tgcctatgtc
    49921 catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg aggcggacgt
    49981 gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa gaaaaaaaac
    50041 aataaataat tggaggattt ttatataatg atggattact taattagtca aaatggtgga
    50101 atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat cggttcagct
    50161 aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca acccgaaaaa
    50221 ttcgggcagg ctctgattct gcaattgctt ccaggtacgc aaggtttgta tggattcgtt
    50281 attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca aggactgaac
    50341 tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat tgcccaagga
    50401 aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc tacaaaagga
    50461 atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat ttccttccta
    50521 ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt gaaaaaatca
    50581 tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa gaagagcgaa
    50641 tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa catcaaaaac
    50701 aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg aatcgccagc
    50761 aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga cgattgtttg
    50821 cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag tttatcaaaa
    50881 atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga gaaaaatcag
    50941 cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc tttatgatgg
    51001 ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat caaggagttc
    51061 agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg agttttgaga
    51121 ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg aactaaatcc
    51181 cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac ggatgattca
    51241 aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca gtccatatat
    51301 ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga gccgtctatt
    51361 tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata cgatgcgtta
    51421 tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa atctggacca
    51481 tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca ttcatacgca
    51541 agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac gtgattattt
    51601 tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt ttttgacaga
    51661 gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag tcattgcttt
    51721 tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa gaacatatgg
    51781 gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat ttttgaactt
    51841 tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt atgctgattt
    51901 tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac agttgaaaga
    51961 tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac cactgccact
    52021 actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac tggttatcgg
    52081 caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag tctatgacgc
    52141 ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg tttggctttg
    52201 atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa atggcaaaga
    52261 atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca gaaacgatcg
    52321 aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat caaggatcat
    52381 tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt caaaatatct
    52441 tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa gtctccggac
    52501 ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt ctagtgggag
    52561 atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct attcaagtat
    52621 atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt gaagctttat
    52681 ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa cggccattag
    52741 atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa ttgcccgcat
    52801 tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa acggtgagtg
    52861 ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa atcatggtgc
    52921 caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact attgatgaaa
    52981 cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt caaaaatggc
    53041 cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc atgatcactg
    53101 gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca gctgtacctg
    53161 gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg gccgatgtgg
    53221 acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat gttttaaatg
    53281 aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt acggtattga
    53341 ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat acgggaatca
    53401 caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca gactccactt
    53461 cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg cctggtgatg
    53521 aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga tctggaagag
    53581 tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt gctgtatccc
    53641 cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc gtaaaagtat
    53701 tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc gattggatac
    53761 aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc cttcaagctg
    53821 actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag cagctgaatg
    53881 aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg acattagaag
    53941 tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat gtcgatacat
    54001 tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt ggtgaagaag
    54061 gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt accgtatcga
    54121 ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa aaactagatc
    54181 aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga ggcatgagca
    54241 atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc ggttgaaaaa
    54301 gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg cgagattcgt
    54361 cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt cgaaggaaca
    54421 agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt agaactaggt
    54481 gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa agataacgga
    54541 ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa tccagtagct
    54601 cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca tttgaataca
    54661 ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca taaagaatta
    54721 gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt cgccgtagtc
    54781 tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga ttttcgtcaa
    54841 acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc tgcaatcgaa
    54901 cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta tgaaaaggga
    54961 atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt gcgagagatt
    55021 tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct ttacacgaac
    55081 ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc ggttactcaa
    55141 attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga cttgactggt
    55201 tatattacag aaggacagat catcttgact agagagcttt acaagagtgg gattcagcca
    55261 ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg acccggaaag
    55321 acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc acaaggaaaa
    55381 caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt agacaagatc
    55441 tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt ctacacgaac
    55501 cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt gccaagaacc
    55561 gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg gaagtgagtt
    55621 ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt gaaaaaacag
    55681 ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga actgatgcgt
    55741 caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga agagtctatg
    55801 acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc tttcatcgaa
    55861 gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa aaatatcatg
    55921 agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga cacacctttg
    55981 gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg gtttactacc
    56041 ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact gatggcgagt
    56101 gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat ccctcaatta
    56161 gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc agaagtaaca
    56221 aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt ttttaaaaga
    56281 ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct gttcagcttc
    56341 tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc ctaccatttt
    56401 ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca acttctgctg
    56461 tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt gctggtcaat
    56521 ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt ccgattctat
    56581 gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta aaagaagcgc
    56641 tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta aaattggcct
    56701 ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct gaatttggct
    56761 ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt aatgcagggt
    56821 ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg atcatggtgg
    56881 tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat ttactggaat
    56941 atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt acaggtaccc
    57001 ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca acgctagtgg
    57061 aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca cctagaacgg
    57121 ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt ttaacgatgt
    57181 ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa acaactacgt
    57241 taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga gcggaagcat
    57301 ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt tttgttacgt
    57361 tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata cctaaaactt
    57421 ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta ggtcttacga
    57481 tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg cttatgtaca
    57541 ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca caaagaacac
    57601 aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat cttctgacag
    57661 aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc ttggatactt
    57721 taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc tcgggtagtg
    57781 cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca ataggttata
    57841 attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa atttcaaggt
    57901 tattagagga tagggaaatt cgaacgaatg catctggttg cgtacgtgta gtaaagcgtc
    57961 tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc ttagggtcaa
    58021 tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt ttttctactt
    58081 tgaaaagaga atcaaaaagc tgattggctc tttttgtaag acttaattta gtttctaaga
    58141 tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct aatccagaag
    58201 ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt actggaacta
    58261 caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct aagtagattg
    58321 tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag taagttgtta
    58381 aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc acttcggcta
    58441 attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa gaaggcataa
    58501 gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca ggtcgcattc
    58561 cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc aattcaatga
    58621 aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa agtgcagaaa
    58681 gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct aaggacggaa
    58741 tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt gtgcctttgg
    58801 gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat tgaataaacg
    58861 ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca atgatatgtt
    58921 cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa ataaatgtgc
    58981 cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa gccttgccaa
    59041 cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt aactgagtgt
    59101 ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac gatcgataat
    59161 aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg tcataagaat
    59221 taataggcat tttctccact ccttaaaatt gtataccata caattttacc attgattgta
    59281 ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt attaattttg
    59341 aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa aaatgttttt
    59401 ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt ttacctgacg
    59461 gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac agatattttg
    59521 gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct tctcatttag
    59581 gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata aacgcaaatg
    59641 cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac ttagcaaatc
    59701 cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg catttgactg
    59761 cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta gaagaaagac
    59821 gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt ataaatgcaa
    59881 tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc tctagtaaag
    59941 aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct gtgggcacat
    60001 ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa atctatgtag
    60061 atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg gcagaaggag
    60121 atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag aaaaaaacat
    60181 tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct attgaagatc
    60241 tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa tgaacttaga
    60301 ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag cagcggttta
    60361 tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg aaaacaattt
    60421 atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat ggtttacgcc
    60481 agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg ttttatttaa
    60541 ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta atctatttgt
    60601 gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga aaaaagtgcc
    60661 gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct acttagcacg
    60721 tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc cagattttac
    60781 agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa gtgtgcaaaa
    60841 agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc cagtatgtgg
    60901 atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa caaaattgtc
    60961 agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag aagatcgtgt
    61021 gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt gatttctttc
    61081 aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat ggttgttaaa
    61141 aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa ttattggtgt
    61201 ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg atgacttcga
    61261 ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca ggtaatcctt
    61321 ttaagtcagt aatctcaaga ccagcagctt tatgttgctc gtgtaatttt tctagatcat
    61381 cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca tgatcatagt
    61441 tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca agtgtaaatt
    61501 tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt tgataaaatt
    61561 cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc atagattcat
    61621 ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt tgatttgtgc
    61681 ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta aggtggaaac
    61741 tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat caaacccgtt
    61801 atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag gctccaaatg
    61861 gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa gaagcgattg
    61921 tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat ctgggagaag
    61981 ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca ggatatttct
    62041 ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat aaaatttggt
    62101 gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga tgggcagtcg
    62161 agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct ttctctcagt
    62221 ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg ctcgtcgttt
    62281 gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc cattgctact
    62341 cctagaccag catattcaat catggataag tcgttctcgt tatctccgat agccatgatt
    62401 tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc tttacttgct
    62461 tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta tttatcacgg
    62521 aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc gatcatcatc
    62581 attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt tagcggcatc
    62641 ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg cgtgtagatc
    62701 gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccatcac ttcgatctct
    62761 aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt cgtttgttgt
    62821 acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt caactcgttt
    62881 aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga aacacctttt
    62941 gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt ctcatttaat
    63001 aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc caatatattt
    63061 taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg ataagtttcg
    63121 tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg aaagaaaaat
    63181 cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac ctgtgataat
    63241 tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct gccttgttct
    63301 gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta ataaacagga
    63361 ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga atcccggttg
    63421 ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc taaatcattt
    63481 aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac cccatcatca
    63541 agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag taattgagaa
    63601 tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg aagcagatgc
    63661 tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac atacatttga
    63721 tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa agcgatcccg
    63781 ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc atattctgtt
    63841 ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat ctgactagaa
    63901 atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc taccttttca
    63961 ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg agaagtgatg
    64021 atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt atgagagtaa
    64081 ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg ttcctgatca
    64141 tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat atcgcagatt
    64201 cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc tgctccgtga
    64261 aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac ttctttcgaa
    64321 ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac tggatcacga
    64381 aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat gtagctcctt
    64441 tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca gctggatttc
    64501 tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct tttcaaaagc
    64561 ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca gttcttcaag
    64621 tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt cttcttttaa
    64681 agcagattga taaaatcgct tcatgacttg cccgcgtttt tcctgattac cgttgacact
    64741 taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac cggcaaattt
    64801 tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga tttcgaatgc
    64861 ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct cgtaagcgtc
    64921 atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat tcaaaattcc
    64981 ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt aacctgcatc
    65041 tttcagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca tacctaagat
    65101 gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt tagaatattc
    65161 cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa tgtgctgatc
    65221 tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat tttctacaat
    65281 attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa aatggacaag
    65341 ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa aggagaatga
    65401 ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa agtaaaacag
    65461 caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac aattggcgat
    65521 acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat tcctgtaacg
    65581 atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat gcggatgcgt
    65641 ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata cggtatgatc
    65701 cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc cacagcaggg
    65761 aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga atattatttg
    65821 accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt ctgattttgt
    65881 atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt atttgaaggt
    65941 gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga acaacgtggc
    66001 gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg gaaatcagac
    66061 agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga cggcagcttt
    66121 atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga ttcgatcgat
    66181 gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg ccgtaaacgc
    66241 aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa tgacgatgac
    66301 ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga cgatgatgat
    66361 gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc agatgacccg
    66421 acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat catagacgaa
    66481 gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa agaataaaca
    66541 ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa ggaggtgtga
    66601 cacgattttt gtcacacctt cttttttgta tataaaaatc ccaagtaaat acataaaaaa
    66661 taaaaaaatt gaaactttta gcgcaccttg tacgtcatat taagtgaggc agaaaggagt
    66721 ggagaatgat ttttactgga tataagaaaa aacggcagat cgaaaaatac cgtcacctgc
    66781 tgaatggact catcgaaact gattatcaaa aaatgttccg tatcacgatg aattacgtac
    66841 atgataaaga agaggcctta gatgtgatgc aagatagttt tcataaagcc ctgaactatt
    66901 ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt ttatcggata ttgatccgca
    66961 cggcattaga tagttggcgc agacaaaaaa ggacgcctgt ggacttattt gaaattcaag
    67021 atatccccca acagttacct ttagaaactt ccgttgcaga gctccatatg attttagata
    67081 aaatagacag tccggaaaaa gagattctga tcctctactt ttttgaagga tttcgtttga
    67141 aagaaattgc gactatcttg gatatgaatg aaaacacagt taaaacgaaa atgtaccgct
    67201 cattgaatgc tttacgtcaa atcctagaat gaaggagaga agagtgccat gtattcccaa
    67261 aaagaaaatt taaaaaaact aaagaaaact tataaagaac aagcaattcc caaagggctt
    67321 aaagaggaga tccgccagcg ttttattatc gaagagcaat catttataaa agcaaagcgg
    67381 agaaagagac gtctccaagg gttcggagta tctttggcta tcgtaatgat tacaacaagt
    67441 agccttcttt ttaatagtca agttcgaagc tttgcagaag atttacccat cttaggatca
    67501 gtcattgaat tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat cgatattcag
    67561 gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa caattcatgg attaaatcaa
    67621 aaatactttc gcgaaggaca agcagatttt gaaaaagctg aaaaagaata tggaaatttt
    67681 gaaacggatc attaccaagt tttgggagat tatcagaaaa tcgtagacga taaccggttt
    67741 ctcgtgattg aaaggcagat cacccaaaca gcagcagata gccatgttga aaaaagatac
    67801 gacacaatcg acaagaaaaa cagtgtccag ctctcacttc cacttttatt caaagatgac
    67861 acctatctat ctgttttgac aaaagaagtc aaacggcaga tggccgaaca agtcaaggaa
    67921 gatccatcta agtactattg gactgagcaa gatattcaag aagggacgat agaaaaacca
    67981 acacttgtta cgcctacacg aagtttttat ttgaacaaag agcatcaatt agttttgact
    68041 ttttctcaat acgaaattgc tcctggttat atgggtacac cagaatttgt cattcctaag
    68101 tccgtgacaa aaacgatttt ggcatcggaa gattatttgg atcattagac gagaagtagt
    68161 tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa taataagaag agaaaaagag
    68221 aaagaagaga tatagtgaaa agaaattatt tagttatcag tattattgct ttatccgtgc
    68281 ttttgttttt cgaaattact tcggtggcct ccttgatcac cgaagtaaaa ggacaaacac
    68341 actcttcgga gcattcgtca caatctttca aacaatcaga acaatcaacc attaaaagcc
    68401 aatcaactga aacagctaac gtcaaagaag ccattcaaga atcagcggaa aaggtacaaa
    68461 gtcagtcatc acaatcaaca caagacagta caaaaacgag ccgagatccg gctgtcaaaa
    68521 gcgtcgcaat cagctttgat gatggaccag gagcgaccac aacaccgcag cttctgcgaa
    68581 tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt aggagaaaat acggcgcagc
    68641 atcctgaaat cgtgaagcag acagctgaag cagggcacga gattggcaat cacacatacg
    68701 atcatcagaa tctggccact ctttctgccc agtcgatgac ggaggaagtg acgaaagcag
    68761 acactgagat caaaaaagtc actggcaaaa cccccacttt tgttcgtccg ccctatggtt
    68821 ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt
    68881 cagaagattg gaagacaaga aatcctgatt tgattttgca gaagatccaa gcaacagtat
    68941 acgatggggc aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc
    69001 aagtgatcga ttatctgaaa gagcaagggt atcgcatcac gacagttggg gatttattag
    69061 ggcatccaac ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag
    69121 tgagacaagt atctttaata aataacacat tttaataagt agttgaacac ataactacct
    69181 tacttgcaaa ggcttaacaa tatctataca ggtacaacaa aaaactgtcc accaattttc
    69241 tttggcggac agttttcact taaaaataaa gaagcaacta ttcgtaaatt atgctaaaat
    69301 agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga atcttacgtg
    69361 atatccacta cactaaggac gcaagcactg ggataattgt aactaaacag ataaaaaatt
    69421 acaaggggat tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa acagcagcag
    69481 acacagaaac tagccatgac gcaacagttg caacagtcca tccaagtttt acaatataac
    69541 agtgaggagc tgttggcatt cgttgagaat caagcaatgg aaaatccatt ggtggaagtt
    69601 gttgagccgg aatggcagcc agattacata aaagcttctt catcttctta tgaaggagaa
    69661 gagacaaatt acctaaatca gattcctgat acaaaagggt ctttatttga ttcattgatc
    69721 gaacaagtcc atttgaatta ccgagatact tttttgagga aaatcgttct ttatcttgta
    69781 gaatacatcg atttgaacgg atttttgacg attagtttag aagaagccgg aaaagagatt
    69841 ggagctacgc ctatccaaat gctcgatgcg ttgactctga ttcagcaatt agaaccagct
    69901 ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg agatgattat
    69961 gcgccggaat tagcttatat cgtgttggaa gaatgttttg aggaactggt ggagcgaaaa
    70021 tggaaagaaa tcgcacaacg tttttctgtt gatctacatg ctgttcagca gatattcgac
    70081 tatttacaaa cgttgagtcc aagccctggc cggattttcg accgtagcag cgaattgtac
    70141 attattcctg atgtccgggt attagtggac gacgataaaa atgtacaagt tatctcaaac
    70201 cgaaaaaatc agccaaatat acgctttcaa gagagttact tcaaacaaat gtcacaacaa
    70261 gcagataaag aaactgaaaa ttatttaaaa gaacggaaac aggaattcga atggttgaag
    70321 aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat tgtttcccgg
    70381 caaaaagctt tctttatcga caaggaacgt ccaatcaagc ctcttacgtt aaaggaagtt
    70441 gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg gaaatatttg
    70501 gaaacagact tcggtgtatt tgaattaaaa aaatttttca ctacacgaat tcctacgaat
    70561 agtacagaac agacagaaga cctttcagca gatacagcga agaaaaagct gcaggaatta
    70621 gtcgatcaag aagacaaaaa caagccgttg tctgaccaga aattggttga attgctgaaa
    70681 aaagatgaga tagcgatctc aagacgaaca gtagcaaaat atcgtgattt attaggaatc
    70741 ccgagttcat caaaaagaaa acgttatgat aactaatcag tcaaaagact agaaaaagta
    70801 tttcactttt tctagtcttt ttagttgaaa aacgagagca aaactgttag acttacagtc
    70861 gtgaggttga gatttccctt tattgatgtg aaattcgaat agttaatttc tttttttatc
    70921 gtatatgggt cgttttaagt ctacattgga cgtaaagtgt ccaactaagg agatcgctat
    70981 gctggatgaa ataaaaatga ttgaatctgt tgcaccagat attatcgaag tgttgcaaga
    71041 acgctataaa attttacgaa atatctattg gatgcagccg attggacgtc gaagtttatc
    71101 ggaaagtatg gggatcacag aacgagtatt gcgaacagaa acagatcttt tgaaaaatct
    71161 gaatttgatc gacacctcaa aaagcggtat gacgctgaca actaagggcg aagaagttta
    71221 tcaaagcctt gaaaacttca tggatcagct tttaggtacg catcaaaccg aacaacagtt
    71281 ggcagaatat tttggtaacc aacgctgtat cgttgtttca ggaaacagcg aagagcagac
    71341 caaagtggct gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac tacttccaga
    71401 aggcgagaat atcatcgctg tgatgggagg aactactatg gctgtggtag ccgaacaatt
    71461 gtctaatctg gaaaataaga aacggcataa cctgtttgtc cctgcaagag gcgggattgg
    71521 tgaagcgata actgtacagg caaattctgt cagtgcaaga atggctgcta aagcaaacgg
    71581 taaccaccgt gcactttatg ttccggaaca attaagtctg gcaacttaca attctttact
    71641 caatgagcca tctatccaag aagtcttaaa cttgattagt gaagcgaatt gtgttattca
    71701 tagtattggg cgtgcattac acatggcagc acgtcgtaag atgactgaaa aagaattagt
    71761 catgctgaaa caagcaaatg ctgttgcgga atcttttggc tactttttca atgaaaaagg
    71821 tgaagtcgtt tataaggtcc ctagaatcgg catagaattg caagatttgg aaaaagtccc
    71881 gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag cttatatgaa
    71941 aaatgcacca aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg agattttaaa
    72001 aggggtaacc ctttaaaata aatatttttt tgattttcat aaggaggaaa tcttaaatga
    72061 cagttaaagt aggtattaat ggttttggac gtattggacg tctagcattc cgtcgtatcc
    72121 aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt gacagatgct aaaatgttgg
    72181 cacacttgtt aaaatacgac acaactcaag gacgtttcaa cggaacagtt gaagttcatg
    72241 aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc aacccagaag
    72301 aattaccatg gggcgaacta ggcgtagata tcgttctaga atgtactggt ttcttcactt
    72361 ctaaatcagc agctgaaaaa catttaacag ctggtgctaa acgcgttgtt atctctgctc
    72421 ctggcggaaa cgatgttcca acaatcgttt ataacacaaa ccacgaaaca ttaactggta
    72481 aagaaacagt tatctcaggt gcttcatgta ctactaactg tttagctcct atggctaaaa
    72541 cattgaacga caaatttggt gttgttgaag gattaatgac aactatccac gcttacacag
    72601 gtgaccaaat gactctagac ggacctcatc ctaaaggtga cttccgccgt gcacgcgctg
    72661 ctgctgcaaa catcgttcct aactcaacag gtgctgctaa agctatcggt ttagtaattc
    72721 ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt tcctgtacca acaggttcat
    72781 taacagaatt agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc aatgcagcaa
    72841 tgaaagaagc ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt tcttcagata
    72901 tcgttggtat gacttacggt tcattatttg atgctacaca aactaaagta atgacagttg
    72961 gcgacaaaca attagttaaa actgttgctt ggtacgacaa cgaaatgtca tacactgcac
    73021 aattagtacg tactttagaa tacttcgcta acttataaga tagacaaacg tacaagtgta
    73081 aattatagac atgtacattt aaaagcgggg aagcaatcgc gcttcttccg ctttttttga
    73141 taataagttt attcaggagg aaataaaatg gctaagaaaa cagtgaaaga tatcgattta
    73201 aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga cggcgtgatc
    73261 actgacgaca ctcgtatcaa agcagcattg ccaacaatca actacgttct agaacaaggc
    73321 ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga taaagaagga
    73381 aaatctttag ctccagttgc aaaacgtcta ggcgaattat taggtaaaga agtaacattc
    73441 gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa tgacggcgac
    73501 gttgtcgtat tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga aagcaaaaat
    73561 gatacagaac taggtaaata ctgggcttca ttaggcgatg tatttgttaa cgatgctttc
    73621 ggtacagctc accgtgcaca tgcttctaac gtaggtatcg cttcaactgg tatcccaaca
    73681 gttgctggat tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt agaagaacca
    73741 aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt ctgataaaat cggcgtaatc
    73801 gaaaacttga ttcctaaagc agataaaatc ttgatcggtg gcggaatgac ttacacattc
    73861 tacgaagcaa aaggaatcaa gatcggtaac tctctagtag aagctgataa agtggaacta
    73921 gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct tgccaatcga caatgtctgt
    73981 gcaccagaat tttcaaatga tgttgaaaca caagtaatcg aaggagacat tcctgatggt
    74041 ttgatggctt tagacatcgg cccagcatca gtaaaattat ttgctgatac attacaaggt
    74101 gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa ctttgctaaa
    74161 ggtacaatcg gtgtttgtga agcaatcgct aacttagaag gcgcaacaac aatcatcggt
    74221 ggcggagact cagccgcagc agcagaacaa ttaggatttg ctgacaaatt cacacacatc
    74281 tcaacaggtg gcggcgcaag cttggaattg cttgaaggta aagaattacc aggactagca
    74341 gcaatcaacg ataaataaac gaccaagttt gctcaattct gacgagtaat cgaacaaaag
    74401 gtgaagggag ttttcatgac tccctagcca tttttcaata aattaaggag tgtgttcctc
    74461 aatgcgtaaa cctattatcg caggtaactg gaaaatgaac aaaactgcat cagaagcaaa
    74521 agaatttgct gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg attcagtcat
    74581 cggatctcct gcattattct tacaagaatt aatgacatca gctgaaggta cagaactaaa
    74641 aatctcagca caaaactgct actgggaaaa ctcaggtgcg ttcactggtg aaacttcacc
    74701 agcagctctt gctgatctag gtgttcatta tgtgatcatc ggacattcag aacgtcgtga
    74761 atatttccat gaaacagacg aagacatcaa taaaaaagcg aaagctatct ttgcaaatgg
    74821 tatgacacca atcctatgct gtggcgaatc tttagaaacc tatgaagcag gcaaaactgc
    74881 tgaatggatc gaaggacaaa ttacagctgg tttagctgat ctttcagctg aacaggtgtc
    74941 aaacatggtt attgcttatg aaccaatctg ggcaatcggt actggtaaat cagctgacgc
    75001 acaaatcgct gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac tttatggaaa
    75061 agaagtatca gaagcagttc gtatccaata cggcggttct gtaaaaccag aaaatattgc
    75121 tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa gcttgcaagc
    75181 agattcattc ttgtcattgt tagacgctgt aaaataattg ttgaaaattt gcgaaaaatg
    75241 caagaaatgt ttgcactaat gagcaaaatt aactaaaatc gtatatgagg gaaatccctt
    75301 aggaaaaaac aaactcaaag gagagacaaa acatgtcaat tattactgat gtttacgctc
    75361 gcgagatctt agactcacgt ggtaacccaa caatcgaagt agaagtatac acagaaagcg
    75421 gagcttttgg ccgcggaatg gttccttcag gtgcttcaac tggtgaatac gaagcagttg
    75481 aattacgtga cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa gctgtcgaca
    75541 acgtaaacaa catcattgct gaagcaatca ttggctacga tgttcgtgac caaatggcta
    75601 tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa aggtaaatta ggtgcgaacg
    75661 ctattttagg tgtttctatc gctgttgcac gtgcagctgc tgactaccta gaagtacctt
    75721 tataccacta cctaggcgga ttcaacacaa aagtattgcc aacaccaatg atgaacatca
    75781 tcaacggcgg atcacatgct gataactcaa tcgacttcca agaattcatg atcatgcctg
    75841 ttggcgctcc aacattcaaa gaagctctac gtatgggtgc tgaagtattc cacgcattag
    75901 cttcaatctt gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt ggatttgctc
    75961 caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc gaaaaagctg
    76021 gctatgtacc tggtaaagac gttgttcttg ctatggatgc tgcttcttca gaattctacg
    76081 acaaagaaaa aggtgtttac gtactagctg attcaggcga aggcgaaaaa acaactgacg
    76141 aaatgatcaa attctacgaa gaattagttt ctaaatatcc aatcatctca atcgaagacg
    76201 gattagacga aaacgactgg gacggattca agaaattaac tgacgtatta ggcgacaaag
    76261 ttcaattagt tggtgacgac ttgttcgtaa caaacactca aaaattatct gaaggtatcg
    76321 aaaaaggaat tgctaactca atccttatca aagtgaacca aatcggtaca ttaacagaaa
    76381 ctttcgaagc tatcgaaatg gctaaagaag ctggctacac agcagttgta tctcaccgtt
    76441 ctggtgaaac agaagattca acaatctctg atatcgctgt tgcaacaaac gctggccaaa
    76501 tcaaaactgg ttctctatca cgtactgacc gtattgctaa atacaaccaa ttactacgta
    76561 tcgaagacca acttggtgaa gttgcagaat acaaaggttt gaaatctttc tacaacttaa
    76621 aaaacaaata attcttttct aatgaagata catgatcgac tattgaaaga tagtcgagta
    76681 tgatcactta gttaagtaat cgaactctct acttgctgaa atatcagcag gtagagagtt
    76741 tttttgcaac tagcttctgt atatgatgtt tacaaagaat ggtacaagaa aagatcatcc
    76801 ttgaaaaagg aaagctaaca ataactagca tcatataaga gcttttaagg atagaacagt
    76861 agaataggaa tctatgagca agtcctctcg tataaataaa ataggaggga agtagcagca
    76921 tatttttcat aaagtggctc tctttcttca tttttacttt ggttgctcct atttgaaaat
    76981 tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga gaggattaga tgaaaaaaga
    77041 agaaaaaatc gcgattttac aagagatcat ccgaattaaa tcagtgaatg gaaacgaagg
    77101 tgaagtagca gcctatttga ataagctttt ggcaaagcat gacataacag gagagattgt
    77161 ttcttacaga gatggtagag ataatttgat tgctcgttac caaaagggcc aatcaggaaa
    77221 agtgttaggc ctatctgggc atatggatgt cgtggcggct ggtgatgaat cgtcttggac
    77281 ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg tacggacgtg gtgctacaga
    77341 tatgaagagt ggcttggcag caatggtgat tgccatgatc gaactgaaag agtcaggaaa
    77401 accatttaac ggaacagtca aattattagc cactgtcgga gaagaagtgg gtgaactagg
    77461 tggggaacag ctcacgaaag caggctatgt agatgactta gatgccttga tcatcggaga
    77521 gccaaccaat tacagtttga tgtatacgca catgggatca atcaactata cagtcacctc
    77581 gcacggaaaa gaagcgcata gctctatgcc agaccaaggt tataatgcaa tcaatcacct
    77641 aaatgaattc attacgaaag caaatgctga aatgaatcac ctcgctgaaa caatcgagaa
    77701 tcctgtccta ggaaaaacaa tccataatgt tacattgatc agtggcggga atcaagtcaa
    77761 cagcatccca agccatgcgc agctccaagg gaacattcga tcgattccag aatacccaaa
    77821 tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa ttgaatcaag agacagacta
    77881 tcatttggaa ttgatgatcg attacaacaa aatacctgtc aaagctgatc cagattctcc
    77941 tttgattcat tgcattcagc aacaattcag ccagccatta cctttagttg gagcggcagc
    78001 aacaacggat gcagccgaat ttacgaaagc taaccattcc ttcgactttg tcgtctttgg
    78061 gcctggtgtc gttactttgc ctcatcaagt agatgagtat gttgaaatcg ataattattt
    78121 ggatatgatc gaaaaatatc aaggaattat tttatcctac ttagcgtaag atttttttaa
    78181 taaatgaaaa aacagagcca gattagcatt a
//
LOCUS       DAZBWR010000003        57037 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000003 DAZBWR010000000
VERSION     DAZBWR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.003"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..180
                     /locus_tag="ACHGT5_000155"
     CDS             <1..180
                     /locus_tag="ACHGT5_000155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216176.1"
                     /translation="KYRVSDFSTTSKNYCFFPLKCLQKVGSGRIYWQNQGEGSLNKEK
                     NHRKYKVITYKTSFL"
     gene            complement(467..970)
                     /locus_tag="ACHGT5_000156"
     CDS             complement(467..970)
                     /locus_tag="ACHGT5_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN7216177.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1329..2354
                     /locus_tag="ACHGT5_000157"
     CDS             1329..2354
                     /locus_tag="ACHGT5_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN7216178.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2677..3555
                     /locus_tag="ACHGT5_000158"
     CDS             2677..3555
                     /locus_tag="ACHGT5_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFN7216179.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4031..4147
                     /locus_tag="ACHGT5_000159"
     CDS             4031..4147
                     /locus_tag="ACHGT5_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN7216180.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5075..5413
                     /locus_tag="ACHGT5_000160"
     CDS             5075..5413
                     /locus_tag="ACHGT5_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216181.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5507..5737
                     /locus_tag="ACHGT5_000161"
     CDS             5507..5737
                     /locus_tag="ACHGT5_000161"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN7216182.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6137..6508
                     /locus_tag="ACHGT5_000162"
     CDS             6137..6508
                     /locus_tag="ACHGT5_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN7216183.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6782..7474)
                     /locus_tag="ACHGT5_000163"
     CDS             complement(6782..7474)
                     /locus_tag="ACHGT5_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7216184.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7477..8262)
                     /locus_tag="ACHGT5_000164"
     CDS             complement(7477..8262)
                     /locus_tag="ACHGT5_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN7216185.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8499..9899
                     /gene="lacG"
                     /locus_tag="ACHGT5_000165"
     CDS             8499..9899
                     /gene="lacG"
                     /locus_tag="ACHGT5_000165"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN7216186.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9924..10241
                     /locus_tag="ACHGT5_000166"
     CDS             9924..10241
                     /locus_tag="ACHGT5_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN7216187.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10254..11927
                     /locus_tag="ACHGT5_000167"
     CDS             10254..11927
                     /locus_tag="ACHGT5_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN7216188.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11941..13572
                     /locus_tag="ACHGT5_000168"
     CDS             11941..13572
                     /locus_tag="ACHGT5_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN7216189.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14063..15628)
                     /gene="guaA"
                     /locus_tag="ACHGT5_000169"
     CDS             complement(14063..15628)
                     /gene="guaA"
                     /locus_tag="ACHGT5_000169"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN7216190.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15687..16610)
                     /gene="coaA"
                     /locus_tag="ACHGT5_000170"
     CDS             complement(15687..16610)
                     /gene="coaA"
                     /locus_tag="ACHGT5_000170"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN7216191.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16806..17411
                     /locus_tag="ACHGT5_000171"
     CDS             16806..17411
                     /locus_tag="ACHGT5_000171"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7216192.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17719..18735
                     /locus_tag="ACHGT5_000172"
     CDS             17719..18735
                     /locus_tag="ACHGT5_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7216193.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18876..20177
                     /locus_tag="ACHGT5_000173"
     CDS             18876..20177
                     /locus_tag="ACHGT5_000173"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN7216194.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20223..20885
                     /gene="deoC"
                     /locus_tag="ACHGT5_000174"
     CDS             20223..20885
                     /gene="deoC"
                     /locus_tag="ACHGT5_000174"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN7216195.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20906..21304
                     /locus_tag="ACHGT5_000175"
     CDS             20906..21304
                     /locus_tag="ACHGT5_000175"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN7216196.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21370..22452
                     /locus_tag="ACHGT5_000176"
     CDS             21370..22452
                     /locus_tag="ACHGT5_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7216197.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22664..24226
                     /locus_tag="ACHGT5_000177"
     CDS             22664..24226
                     /locus_tag="ACHGT5_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216198.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24219..25352
                     /locus_tag="ACHGT5_000178"
     CDS             24219..25352
                     /locus_tag="ACHGT5_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216199.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25352..26308
                     /locus_tag="ACHGT5_000179"
     CDS             25352..26308
                     /locus_tag="ACHGT5_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216200.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26407..27573
                     /gene="deoB"
                     /locus_tag="ACHGT5_000180"
     CDS             26407..27573
                     /gene="deoB"
                     /locus_tag="ACHGT5_000180"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN7216201.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27597..28415
                     /locus_tag="ACHGT5_000181"
     CDS             27597..28415
                     /locus_tag="ACHGT5_000181"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN7216202.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28432..29139
                     /gene="deoD"
                     /locus_tag="ACHGT5_000182"
     CDS             28432..29139
                     /gene="deoD"
                     /locus_tag="ACHGT5_000182"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN7216203.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29437..30123)
                     /gene="gpmA"
                     /locus_tag="ACHGT5_000183"
     CDS             complement(29437..30123)
                     /gene="gpmA"
                     /locus_tag="ACHGT5_000183"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN7216204.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30295..30876)
                     /locus_tag="ACHGT5_000184"
     CDS             complement(30295..30876)
                     /locus_tag="ACHGT5_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7216205.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31009..31692)
                     /gene="rpiA"
                     /locus_tag="ACHGT5_000185"
     CDS             complement(31009..31692)
                     /gene="rpiA"
                     /locus_tag="ACHGT5_000185"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN7216206.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32011..32424
                     /gene="rpsL"
                     /locus_tag="ACHGT5_000186"
     CDS             32011..32424
                     /gene="rpsL"
                     /locus_tag="ACHGT5_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN7216207.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32508..32978
                     /gene="rpsG"
                     /locus_tag="ACHGT5_000187"
     CDS             32508..32978
                     /gene="rpsG"
                     /locus_tag="ACHGT5_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN7216208.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33072..35159
                     /gene="fusA"
                     /locus_tag="ACHGT5_000188"
     CDS             33072..35159
                     /gene="fusA"
                     /locus_tag="ACHGT5_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN7216209.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35331..36518
                     /gene="tuf"
                     /locus_tag="ACHGT5_000189"
     CDS             35331..36518
                     /gene="tuf"
                     /locus_tag="ACHGT5_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN7216210.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36925..37233
                     /gene="rpsJ"
                     /locus_tag="ACHGT5_000190"
     CDS             36925..37233
                     /gene="rpsJ"
                     /locus_tag="ACHGT5_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN7216211.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37264..37893
                     /gene="rplC"
                     /locus_tag="ACHGT5_000191"
     CDS             37264..37893
                     /gene="rplC"
                     /locus_tag="ACHGT5_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN7216212.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37921..38544
                     /gene="rplD"
                     /locus_tag="ACHGT5_000192"
     CDS             37921..38544
                     /gene="rplD"
                     /locus_tag="ACHGT5_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN7216213.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38544..38834
                     /gene="rplW"
                     /locus_tag="ACHGT5_000193"
     CDS             38544..38834
                     /gene="rplW"
                     /locus_tag="ACHGT5_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN7216214.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38873..39706
                     /gene="rplB"
                     /locus_tag="ACHGT5_000194"
     CDS             38873..39706
                     /gene="rplB"
                     /locus_tag="ACHGT5_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN7216215.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39749..40027
                     /gene="rpsS"
                     /locus_tag="ACHGT5_000195"
     CDS             39749..40027
                     /gene="rpsS"
                     /locus_tag="ACHGT5_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN7216216.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40050..40397
                     /gene="rplV"
                     /locus_tag="ACHGT5_000196"
     CDS             40050..40397
                     /gene="rplV"
                     /locus_tag="ACHGT5_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN7216217.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40411..41067
                     /gene="rpsC"
                     /locus_tag="ACHGT5_000197"
     CDS             40411..41067
                     /gene="rpsC"
                     /locus_tag="ACHGT5_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN7216218.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41070..41504
                     /gene="rplP"
                     /locus_tag="ACHGT5_000198"
     CDS             41070..41504
                     /gene="rplP"
                     /locus_tag="ACHGT5_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN7216219.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41494..41682
                     /gene="rpmC"
                     /locus_tag="ACHGT5_000199"
     CDS             41494..41682
                     /gene="rpmC"
                     /locus_tag="ACHGT5_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN7216220.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41707..41973
                     /gene="rpsQ"
                     /locus_tag="ACHGT5_000200"
     CDS             41707..41973
                     /gene="rpsQ"
                     /locus_tag="ACHGT5_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN7216221.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42031..42399
                     /gene="rplN"
                     /locus_tag="ACHGT5_000201"
     CDS             42031..42399
                     /gene="rplN"
                     /locus_tag="ACHGT5_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN7216222.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42436..42744
                     /gene="rplX"
                     /locus_tag="ACHGT5_000202"
     CDS             42436..42744
                     /gene="rplX"
                     /locus_tag="ACHGT5_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN7216223.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42772..43311
                     /gene="rplE"
                     /locus_tag="ACHGT5_000203"
     CDS             42772..43311
                     /gene="rplE"
                     /locus_tag="ACHGT5_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN7216224.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43330..43515
                     /locus_tag="ACHGT5_000204"
     CDS             43330..43515
                     /locus_tag="ACHGT5_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN7216225.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43551..43949
                     /gene="rpsH"
                     /locus_tag="ACHGT5_000205"
     CDS             43551..43949
                     /gene="rpsH"
                     /locus_tag="ACHGT5_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN7216226.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43981..44517
                     /gene="rplF"
                     /locus_tag="ACHGT5_000206"
     CDS             43981..44517
                     /gene="rplF"
                     /locus_tag="ACHGT5_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN7216227.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44678..45034
                     /gene="rplR"
                     /locus_tag="ACHGT5_000207"
     CDS             44678..45034
                     /gene="rplR"
                     /locus_tag="ACHGT5_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN7216228.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45055..45555
                     /gene="rpsE"
                     /locus_tag="ACHGT5_000208"
     CDS             45055..45555
                     /gene="rpsE"
                     /locus_tag="ACHGT5_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN7216229.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45570..45749
                     /gene="rpmD"
                     /locus_tag="ACHGT5_000209"
     CDS             45570..45749
                     /gene="rpmD"
                     /locus_tag="ACHGT5_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN7216230.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45793..46233
                     /gene="rplO"
                     /locus_tag="ACHGT5_000210"
     CDS             45793..46233
                     /gene="rplO"
                     /locus_tag="ACHGT5_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN7216231.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46233..47528
                     /gene="secY"
                     /locus_tag="ACHGT5_000211"
     CDS             46233..47528
                     /gene="secY"
                     /locus_tag="ACHGT5_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN7216232.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47590..48237
                     /locus_tag="ACHGT5_000212"
     CDS             47590..48237
                     /locus_tag="ACHGT5_000212"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN7216233.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48428..48646
                     /gene="infA"
                     /locus_tag="ACHGT5_000213"
     CDS             48428..48646
                     /gene="infA"
                     /locus_tag="ACHGT5_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN7216234.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48679..48795
                     /gene="rpmJ"
                     /locus_tag="ACHGT5_000214"
     CDS             48679..48795
                     /gene="rpmJ"
                     /locus_tag="ACHGT5_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN7216235.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48813..49178
                     /gene="rpsM"
                     /locus_tag="ACHGT5_000215"
     CDS             48813..49178
                     /gene="rpsM"
                     /locus_tag="ACHGT5_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN7216236.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49206..49595
                     /gene="rpsK"
                     /locus_tag="ACHGT5_000216"
     CDS             49206..49595
                     /gene="rpsK"
                     /locus_tag="ACHGT5_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN7216237.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49675..50613
                     /locus_tag="ACHGT5_000217"
     CDS             49675..50613
                     /locus_tag="ACHGT5_000217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN7216238.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50641..51021
                     /gene="rplQ"
                     /locus_tag="ACHGT5_000218"
     CDS             50641..51021
                     /gene="rplQ"
                     /locus_tag="ACHGT5_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN7216239.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51293..52132
                     /locus_tag="ACHGT5_000219"
     CDS             51293..52132
                     /locus_tag="ACHGT5_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN7216240.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52108..52977
                     /locus_tag="ACHGT5_000220"
     CDS             52108..52977
                     /locus_tag="ACHGT5_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN7216241.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52967..53764
                     /locus_tag="ACHGT5_000221"
     CDS             52967..53764
                     /locus_tag="ACHGT5_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN7216242.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53967..54713
                     /gene="truA"
                     /locus_tag="ACHGT5_000222"
     CDS             53967..54713
                     /gene="truA"
                     /locus_tag="ACHGT5_000222"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN7216243.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55377..55571
                     /locus_tag="ACHGT5_000223"
                     /pseudo
     CDS             55377..55571
                     /locus_tag="ACHGT5_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55759..56535)
                     /locus_tag="ACHGT5_000224"
     CDS             complement(55759..56535)
                     /locus_tag="ACHGT5_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN7216244.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56658..>57037
                     /locus_tag="ACHGT5_000225"
     CDS             56658..>57037
                     /locus_tag="ACHGT5_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFN7216245.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYA"
BASE COUNT        18233 a        10176 c        12168 g        16460 t
ORIGIN      
        1 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
       61 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
      121 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
      181 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
      241 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
      301 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
      361 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
      421 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
      481 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
      541 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
      601 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
      661 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
      721 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
      781 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
      841 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
      901 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
      961 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
     1021 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
     1081 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
     1141 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
     1201 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
     1261 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
     1321 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
     1381 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
     1441 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
     1501 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
     1561 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
     1621 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
     1681 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
     1741 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
     1801 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
     1861 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
     1921 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
     1981 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
     2041 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
     2101 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
     2161 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
     2221 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
     2281 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
     2341 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
     2401 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
     2461 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
     2521 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
     2581 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
     2641 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
     2701 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
     2761 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
     2821 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
     2881 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
     2941 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
     3001 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
     3061 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
     3121 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
     3181 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
     3241 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
     3301 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
     3361 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
     3421 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
     3481 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
     3541 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
     3601 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
     3661 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
     3721 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
     3781 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
     3841 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
     3901 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
     3961 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
     4021 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
     4081 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
     4141 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
     4201 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
     4261 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
     4321 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
     4381 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
     4441 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
     4501 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
     4561 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
     4621 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
     4681 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
     4741 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
     4801 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
     4861 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
     4921 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
     4981 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
     5041 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
     5101 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
     5161 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
     5221 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
     5281 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
     5341 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
     5401 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
     5461 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
     5521 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
     5581 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
     5641 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
     5701 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
     5761 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
     5821 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
     5881 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
     5941 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
     6001 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
     6061 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
     6121 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
     6181 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
     6241 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
     6301 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
     6361 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
     6421 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
     6481 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
     6541 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
     6601 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
     6661 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
     6721 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
     6781 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
     6841 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
     6901 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
     6961 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
     7021 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
     7081 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
     7141 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
     7201 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
     7261 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
     7321 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
     7381 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
     7441 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
     7501 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
     7561 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
     7621 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
     7681 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
     7741 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
     7801 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
     7861 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
     7921 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
     7981 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
     8041 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
     8101 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
     8161 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
     8221 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
     8281 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
     8341 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
     8401 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
     8461 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
     8521 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
     8581 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
     8641 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
     8701 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
     8761 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
     8821 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
     8881 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
     8941 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
     9001 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
     9061 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
     9121 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
     9181 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
     9241 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
     9301 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
     9361 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
     9421 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
     9481 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
     9541 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
     9601 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
     9661 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
     9721 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
     9781 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
     9841 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
     9901 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
     9961 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    10021 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    10081 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    10141 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    10201 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    10261 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    10321 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    10381 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    10441 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    10501 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    10561 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    10621 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    10681 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    10741 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    10801 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    10861 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    10921 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    10981 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    11041 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    11101 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    11161 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    11221 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    11281 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    11341 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    11401 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    11461 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    11521 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    11581 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    11641 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    11701 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    11761 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    11821 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    11881 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    11941 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    12001 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    12061 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    12121 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    12181 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    12241 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    12301 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    12361 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    12421 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    12481 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    12541 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    12601 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    12661 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    12721 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    12781 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    12841 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    12901 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    12961 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    13021 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    13081 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    13141 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    13201 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    13261 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    13321 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    13381 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    13441 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    13501 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    13561 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    13621 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    13681 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    13741 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    13801 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    13861 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    13921 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    13981 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    14041 ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga
    14101 tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt
    14161 gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag
    14221 cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga
    14281 tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt
    14341 cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc
    14401 caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag
    14461 gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt
    14521 cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg
    14581 tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga
    14641 aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt
    14701 cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga
    14761 agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca
    14821 ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt
    14881 ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa
    14941 gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga
    15001 taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca
    15061 gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat
    15121 tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa
    15181 agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg
    15241 gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt
    15301 cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat
    15361 agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat
    15421 tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt
    15481 ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa
    15541 tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa
    15601 cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct
    15661 caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata
    15721 tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc
    15781 tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca
    15841 cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt
    15901 aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag
    15961 acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta
    16021 ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg
    16081 atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca
    16141 cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc
    16201 atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc
    16261 tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta
    16321 ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat
    16381 ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa
    16441 atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc
    16501 aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat
    16561 tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc
    16621 tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa
    16681 gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt
    16741 gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg
    16801 aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat
    16861 gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac
    16921 gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac
    16981 ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg
    17041 catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag
    17101 cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct
    17161 atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg
    17221 gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta
    17281 cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag
    17341 aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg
    17401 tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg
    17461 tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt
    17521 tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata
    17581 cagaacttgt atagaagact gccaagtcgg tagataaagg aggtttttat caaaaaacat
    17641 actttccttt tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga
    17701 tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta
    17761 tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca
    17821 agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca
    17881 aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc
    17941 ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc
    18001 atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc
    18061 caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca
    18121 ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt
    18181 cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg
    18241 tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa
    18301 ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat
    18361 gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa
    18421 ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga
    18481 tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga
    18541 ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca
    18601 agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa
    18661 agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta
    18721 ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag
    18781 tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat
    18841 gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa
    18901 aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata
    18961 cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag
    19021 acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca
    19081 tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg
    19141 acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa
    19201 tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg
    19261 gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag
    19321 cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg
    19381 acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa
    19441 tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga
    19501 agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta
    19561 accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa
    19621 atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag
    19681 tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa
    19741 ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat
    19801 tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa
    19861 ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa
    19921 tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag
    19981 aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga
    20041 caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg
    20101 aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg
    20161 acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag
    20221 aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag
    20281 ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc
    20341 cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag
    20401 taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg
    20461 ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa
    20521 aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg
    20581 cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt
    20641 gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag
    20701 gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg
    20761 tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg
    20821 ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct
    20881 attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc
    20941 tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct
    21001 cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt
    21061 aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt
    21121 tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg
    21181 acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg
    21241 tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct
    21301 ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc
    21361 tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg
    21421 ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca
    21481 tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc
    21541 aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga
    21601 aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac
    21661 caagcattga atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg
    21721 atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc
    21781 gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca
    21841 ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa
    21901 ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa
    21961 gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat
    22021 aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct
    22081 tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac
    22141 aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta
    22201 aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta
    22261 gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat
    22321 ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa
    22381 gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct
    22441 gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt
    22501 tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa
    22561 caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt
    22621 caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat
    22681 tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat
    22741 caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa
    22801 atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat
    22861 gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat
    22921 ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg
    22981 aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa
    23041 agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt
    23101 tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat
    23161 ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat
    23221 gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat
    23281 caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa
    23341 cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt
    23401 caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt
    23461 ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg
    23521 cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt
    23581 gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa
    23641 taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa
    23701 atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca
    23761 aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga
    23821 attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc
    23881 aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt
    23941 gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg
    24001 tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga
    24061 aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt
    24121 tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga
    24181 agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat
    24241 tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat
    24301 tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc
    24361 ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag
    24421 cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag
    24481 cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag
    24541 ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg
    24601 ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga
    24661 actatatctt attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc
    24721 gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt
    24781 taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc
    24841 ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg
    24901 gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt
    24961 cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt
    25021 atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt
    25081 ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt
    25141 tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg
    25201 tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg
    25261 ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa
    25321 cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat
    25381 ccttacttct acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt
    25441 ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt
    25501 tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat
    25561 cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac
    25621 agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc
    25681 tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca
    25741 gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt
    25801 ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt
    25861 gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc
    25921 tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg
    25981 cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc
    26041 tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa
    26101 tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat
    26161 tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata
    26221 tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa
    26281 cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg
    26341 aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga
    26401 ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca
    26461 ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa
    26521 gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa
    26581 ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta
    26641 gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt
    26701 cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc
    26761 cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa
    26821 caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc
    26881 gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt
    26941 gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt
    27001 gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga
    27061 cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc
    27121 aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat
    27181 ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat
    27241 ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg
    27301 attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg
    27361 ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga
    27421 gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa
    27481 ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa
    27541 aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga
    27601 ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag
    27661 aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag
    27721 atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc
    27781 atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga
    27841 gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg
    27901 ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat
    27961 ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt
    28021 tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata
    28081 cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag
    28141 aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt
    28201 ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag
    28261 ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc
    28321 aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta
    28381 aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg
    28441 catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg
    28501 cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc
    28561 ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga
    28621 atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag
    28681 aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta
    28741 gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt
    28801 cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa
    28861 aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat
    28921 ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg
    28981 gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc
    29041 gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa
    29101 atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc
    29161 ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg
    29221 gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc
    29281 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    29341 tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg
    29401 acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac
    29461 tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat
    29521 gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg
    29581 tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa
    29641 tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc
    29701 taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg
    29761 aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc
    29821 tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga
    29881 tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat
    29941 tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt
    30001 gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc
    30061 agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg
    30121 catgtttagc tctcctttta agataggttt atatttctga aattttcagt tctgttttat
    30181 tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca
    30241 agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat
    30301 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
    30361 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
    30421 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
    30481 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
    30541 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
    30601 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
    30661 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
    30721 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
    30781 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
    30841 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
    30901 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
    30961 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
    31021 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
    31081 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
    31141 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
    31201 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
    31261 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
    31321 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
    31381 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
    31441 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
    31501 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
    31561 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
    31621 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
    31681 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
    31741 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
    31801 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
    31861 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
    31921 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
    31981 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
    32041 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
    32101 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
    32161 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
    32221 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
    32281 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
    32341 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
    32401 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
    32461 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
    32521 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
    32581 gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata
    32641 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
    32701 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
    32761 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
    32821 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
    32881 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
    32941 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
    33001 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
    33061 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
    33121 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
    33181 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
    33241 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
    33301 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
    33361 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
    33421 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
    33481 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
    33541 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
    33601 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
    33661 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
    33721 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
    33781 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
    33841 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
    33901 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
    33961 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
    34021 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
    34081 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
    34141 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
    34201 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
    34261 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
    34321 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
    34381 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
    34441 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
    34501 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
    34561 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
    34621 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
    34681 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
    34741 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
    34801 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
    34861 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
    34921 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
    34981 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
    35041 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
    35101 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
    35161 ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
    35221 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
    35281 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
    35341 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
    35401 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
    35461 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
    35521 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
    35581 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
    35641 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
    35701 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
    35761 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
    35821 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
    35881 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
    35941 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
    36001 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
    36061 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
    36121 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
    36181 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
    36241 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
    36301 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
    36361 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
    36421 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
    36481 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
    36541 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
    36601 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
    36661 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
    36721 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
    36781 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
    36841 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
    36901 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
    36961 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
    37021 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
    37081 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
    37141 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
    37201 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
    37261 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
    37321 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
    37381 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
    37441 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
    37501 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
    37561 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
    37621 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
    37681 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
    37741 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
    37801 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
    37861 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
    37921 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
    37981 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
    38041 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
    38101 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
    38161 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
    38221 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
    38281 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
    38341 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
    38401 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
    38461 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
    38521 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
    38581 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
    38641 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
    38701 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
    38761 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
    38821 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
    38881 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
    38941 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
    39001 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
    39061 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
    39121 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
    39181 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
    39241 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
    39301 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
    39361 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
    39421 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
    39481 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
    39541 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
    39601 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
    39661 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
    39721 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
    39781 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
    39841 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
    39901 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
    39961 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
    40021 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
    40081 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
    40141 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
    40201 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
    40261 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    40321 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    40381 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    40441 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    40501 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    40561 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    40621 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    40681 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    40741 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    40801 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    40861 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    40921 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    40981 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    41041 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    41101 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    41161 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    41221 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    41281 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    41341 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    41401 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    41461 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    41521 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    41581 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    41641 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    41701 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    41761 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    41821 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    41881 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    41941 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    42001 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    42061 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    42121 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    42181 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    42241 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    42301 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    42361 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    42421 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    42481 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    42541 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    42601 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    42661 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    42721 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    42781 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    42841 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    42901 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    42961 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    43021 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    43081 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    43141 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    43201 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    43261 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    43321 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    43381 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    43441 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    43501 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    43561 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    43621 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    43681 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    43741 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    43801 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    43861 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    43921 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    43981 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    44041 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    44101 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    44161 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    44221 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    44281 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    44341 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    44401 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    44461 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    44521 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    44581 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    44641 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    44701 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    44761 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    44821 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    44881 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    44941 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    45001 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    45061 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    45121 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    45181 aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    45241 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    45301 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    45361 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    45421 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    45481 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    45541 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    45601 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    45661 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    45721 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    45781 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    45841 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    45901 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    45961 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    46021 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    46081 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    46141 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    46201 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    46261 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    46321 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    46381 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    46441 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    46501 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    46561 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    46621 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    46681 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    46741 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    46801 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    46861 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    46921 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    46981 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    47041 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    47101 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    47161 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    47221 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    47281 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    47341 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    47401 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    47461 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    47521 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    47581 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    47641 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    47701 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    47761 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    47821 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    47881 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    47941 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    48001 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    48061 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    48121 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    48181 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    48241 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    48301 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    48361 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    48421 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    48481 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    48541 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    48601 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    48661 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    48721 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    48781 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    48841 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    48901 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    48961 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    49021 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    49081 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    49141 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    49201 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    49261 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    49321 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    49381 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    49441 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    49501 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    49561 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    49621 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    49681 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    49741 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    49801 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    49861 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    49921 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    49981 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    50041 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    50101 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    50161 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    50221 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    50281 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    50341 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    50401 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    50461 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    50521 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    50581 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    50641 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    50701 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    50761 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    50821 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    50881 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    50941 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    51001 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    51061 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    51121 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    51181 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    51241 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    51301 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    51361 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    51421 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    51481 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    51541 tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    51601 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    51661 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    51721 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    51781 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    51841 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    51901 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    51961 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    52021 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    52081 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    52141 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    52201 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    52261 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    52321 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    52381 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    52441 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    52501 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    52561 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    52621 ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt ttggcgtctt
    52681 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    52741 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    52801 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    52861 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    52921 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    52981 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    53041 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    53101 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    53161 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    53221 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    53281 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    53341 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    53401 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    53461 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    53521 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    53581 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    53641 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    53701 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttgagagg
    53761 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    53821 gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    53881 tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat tccagataac
    53941 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcatacgacg
    54001 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    54061 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    54121 cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca gaggagcgtc
    54181 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    54241 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    54301 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    54361 ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    54421 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta cgtacgatct
    54481 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    54541 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    54601 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    54661 gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggttg
    54721 tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaaat
    54781 tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg aatcagaggt
    54841 tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata gctcgtcata
    54901 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    54961 aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct
    55021 catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa
    55081 agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa aaaacagctt
    55141 ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt
    55201 gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttaatga
    55261 ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt tctaattatt
    55321 tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact acttaagatg
    55381 aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa aagaaaacga
    55441 ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc aagtatcgag
    55501 acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat ctgaaaaagt
    55561 tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa aatagcaaaa
    55621 gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat tcttaaagta
    55681 cttaaactaa gtactttatt ttactcttta ggattgatac agtagcatct atccctttat
    55741 taataatctt ttattttttt aaataaagat gttgtctcca tcttcaggca ctaaaacttt
    55801 ttcggaaatt cctttttctt tattgaaaga ctttaattct tctcttgaca gtccccaatg
    55861 gtttacagct tccatatgaa cggcaataat tgtggaatct ggtattgctt tatgcaattc
    55921 atataaatcg tctttcccca taataagaga ttctccaact aaaaaatgat tatctccagc
    55981 atttgctact atgatttctg gattatactg atcaatagtt tgtttaacag cttcatacca
    56041 aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa caataccgca
    56101 aacattacct acataattca agatttctcc tctgccatgt ttgccttttg ttttaaccaa
    56161 ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc acatttttaa aaccatatag
    56221 tcgaatttca ttcgcatcat tttcatcttg cacaaataat tttatttctt taggtaggac
    56281 ctctttcgct atatcgtccc aatgatctag atgtaaatgt gttacaataa cagcatcaat
    56341 accgttgata atatcttcga ctggcattgg caaatcaact agaggattca tttgatcttt
    56401 tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt tttccgctaa
    56461 cattggatca attaaaaatt ttttacctcc atagtctaag atagctgttg cattacgaat
    56521 gtgatgtata ttcataaacg ttgtctcctt tttgttgata tgaaatattt ttagtataat
    56581 tcaattccat acaataaata aaccgaacaa aaaaagtctt ttactatttt tacttaattt
    56641 atgaaaggtt gtttaccatg ttagaccaaa cagatataaa tatcattgag gaactatcaa
    56701 aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt acatttgact ggacccgctg
    56761 tctcggcgag agtgacaaaa ctagaggaaa gtggagttat tgaaaattat acagtaaaat
    56821 taaatatgga aaagctaggg ttttcgattc acgctttttt aattataatt acgcaaaatt
    56881 taaatcataa accctattta tcatttatta aaaatcatga aaaattcttg attagaaatt
    56941 ataagattag tggagggggc tgttatcttt tggaatgtag atttacttca aaaaaatcaa
    57001 tgaacagctt tttggaacag cttaatgaat atgcgaa
//
LOCUS       DAZBWR010000004        53103 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000004 DAZBWR010000000
VERSION     DAZBWR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.004"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..907)
                     /locus_tag="ACHGT5_000226"
     CDS             complement(32..907)
                     /locus_tag="ACHGT5_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN7216246.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="ACHGT5_000227"
     CDS             1055..1387
                     /locus_tag="ACHGT5_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN7216247.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="ACHGT5_000228"
     CDS             1499..2707
                     /locus_tag="ACHGT5_000228"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN7216248.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="ACHGT5_000229"
     CDS             2861..3316
                     /locus_tag="ACHGT5_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216249.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="ACHGT5_000230"
     CDS             3678..3953
                     /locus_tag="ACHGT5_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216250.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..6044
                     /locus_tag="ACHGT5_000231"
     CDS             3966..6044
                     /locus_tag="ACHGT5_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN7216251.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6207..8090
                     /locus_tag="ACHGT5_000232"
     CDS             6207..8090
                     /locus_tag="ACHGT5_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN7216252.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            8102..9049
                     /locus_tag="ACHGT5_000233"
     CDS             8102..9049
                     /locus_tag="ACHGT5_000233"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN7216253.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9069..9596
                     /locus_tag="ACHGT5_000234"
     CDS             9069..9596
                     /locus_tag="ACHGT5_000234"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN7216254.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9659..10312)
                     /locus_tag="ACHGT5_000235"
     CDS             complement(9659..10312)
                     /locus_tag="ACHGT5_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN7216255.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10444..11286
                     /locus_tag="ACHGT5_000236"
     CDS             10444..11286
                     /locus_tag="ACHGT5_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN7216256.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11444..12340
                     /locus_tag="ACHGT5_000237"
     CDS             11444..12340
                     /locus_tag="ACHGT5_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN7216257.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12343..13056
                     /locus_tag="ACHGT5_000238"
     CDS             12343..13056
                     /locus_tag="ACHGT5_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN7216258.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13076..13594
                     /gene="msrA"
                     /locus_tag="ACHGT5_000239"
     CDS             13076..13594
                     /gene="msrA"
                     /locus_tag="ACHGT5_000239"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN7216259.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13591..13818
                     /locus_tag="ACHGT5_000240"
     CDS             13591..13818
                     /locus_tag="ACHGT5_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN7216260.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13970..14521
                     /gene="lepB"
                     /locus_tag="ACHGT5_000241"
     CDS             13970..14521
                     /gene="lepB"
                     /locus_tag="ACHGT5_000241"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN7216261.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14604..15146)
                     /locus_tag="ACHGT5_000242"
     CDS             complement(14604..15146)
                     /locus_tag="ACHGT5_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN7216262.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15383..15781)
                     /locus_tag="ACHGT5_000243"
     CDS             complement(15383..15781)
                     /locus_tag="ACHGT5_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7216263.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16041..16901
                     /gene="ylqF"
                     /locus_tag="ACHGT5_000244"
     CDS             16041..16901
                     /gene="ylqF"
                     /locus_tag="ACHGT5_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN7216264.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            16894..17661
                     /locus_tag="ACHGT5_000245"
     CDS             16894..17661
                     /locus_tag="ACHGT5_000245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN7216265.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            17717..18574
                     /gene="dprA"
                     /locus_tag="ACHGT5_000246"
     CDS             17717..18574
                     /gene="dprA"
                     /locus_tag="ACHGT5_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN7216266.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            18696..20774
                     /gene="topA"
                     /locus_tag="ACHGT5_000247"
     CDS             18696..20774
                     /gene="topA"
                     /locus_tag="ACHGT5_000247"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN7216267.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            20743..22119
                     /gene="trmFO"
                     /locus_tag="ACHGT5_000248"
     CDS             20743..22119
                     /gene="trmFO"
                     /locus_tag="ACHGT5_000248"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN7216268.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            22317..23222
                     /gene="xerC"
                     /locus_tag="ACHGT5_000249"
     CDS             22317..23222
                     /gene="xerC"
                     /locus_tag="ACHGT5_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN7216269.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            23259..23807
                     /gene="hslV"
                     /locus_tag="ACHGT5_000250"
     CDS             23259..23807
                     /gene="hslV"
                     /locus_tag="ACHGT5_000250"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN7216270.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            23821..25221
                     /gene="hslU"
                     /locus_tag="ACHGT5_000251"
     CDS             23821..25221
                     /gene="hslU"
                     /locus_tag="ACHGT5_000251"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN7216271.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            25239..26030
                     /gene="codY"
                     /locus_tag="ACHGT5_000252"
     CDS             25239..26030
                     /gene="codY"
                     /locus_tag="ACHGT5_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN7216272.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            26112..26987
                     /locus_tag="ACHGT5_000253"
     CDS             26112..26987
                     /locus_tag="ACHGT5_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN7216273.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACHGT5_000254"
     CDS             complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACHGT5_000254"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN7216274.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            27884..28318
                     /locus_tag="ACHGT5_000255"
     CDS             27884..28318
                     /locus_tag="ACHGT5_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN7216275.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            28484..30529
                     /gene="parE"
                     /locus_tag="ACHGT5_000256"
     CDS             28484..30529
                     /gene="parE"
                     /locus_tag="ACHGT5_000256"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN7216276.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            30541..32991
                     /gene="parC"
                     /locus_tag="ACHGT5_000257"
     CDS             30541..32991
                     /gene="parC"
                     /locus_tag="ACHGT5_000257"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN7216277.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            33268..35499
                     /gene="pflB"
                     /locus_tag="ACHGT5_000258"
     CDS             33268..35499
                     /gene="pflB"
                     /locus_tag="ACHGT5_000258"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN7216278.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            35743..36504
                     /gene="pflA"
                     /locus_tag="ACHGT5_000259"
     CDS             35743..36504
                     /gene="pflA"
                     /locus_tag="ACHGT5_000259"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN7216279.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            36589..37515
                     /locus_tag="ACHGT5_000260"
     CDS             36589..37515
                     /locus_tag="ACHGT5_000260"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN7216280.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            37697..38590
                     /locus_tag="ACHGT5_000261"
     CDS             37697..38590
                     /locus_tag="ACHGT5_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN7216281.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            38724..38849
                     /locus_tag="ACHGT5_000262"
     CDS             38724..38849
                     /locus_tag="ACHGT5_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN7216282.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(38983..39615)
                     /locus_tag="ACHGT5_000263"
     CDS             complement(38983..39615)
                     /locus_tag="ACHGT5_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216283.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(39763..40305)
                     /locus_tag="ACHGT5_000264"
     CDS             complement(39763..40305)
                     /locus_tag="ACHGT5_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN7216284.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            40440..40829
                     /locus_tag="ACHGT5_000265"
     CDS             40440..40829
                     /locus_tag="ACHGT5_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216285.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            40833..41597
                     /locus_tag="ACHGT5_000266"
     CDS             40833..41597
                     /locus_tag="ACHGT5_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN7216286.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            41726..42673
                     /locus_tag="ACHGT5_000267"
     CDS             41726..42673
                     /locus_tag="ACHGT5_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7216287.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            42803..43543
                     /locus_tag="ACHGT5_000268"
     CDS             42803..43543
                     /locus_tag="ACHGT5_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216288.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(43834..44502)
                     /locus_tag="ACHGT5_000269"
     CDS             complement(43834..44502)
                     /locus_tag="ACHGT5_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN7216289.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            44636..44923
                     /locus_tag="ACHGT5_000270"
     CDS             44636..44923
                     /locus_tag="ACHGT5_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216290.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            45339..46877
                     /locus_tag="ACHGT5_000271"
     CDS             45339..46877
                     /locus_tag="ACHGT5_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN7216291.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            46874..47839
                     /locus_tag="ACHGT5_000272"
     CDS             46874..47839
                     /locus_tag="ACHGT5_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216292.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            47826..49148
                     /locus_tag="ACHGT5_000273"
     CDS             47826..49148
                     /locus_tag="ACHGT5_000273"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN7216293.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      49211..49315
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            49389..50132
                     /locus_tag="ACHGT5_000274"
     CDS             49389..50132
                     /locus_tag="ACHGT5_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216294.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            50147..51139
                     /locus_tag="ACHGT5_000275"
     CDS             50147..51139
                     /locus_tag="ACHGT5_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7216295.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            51151..51888
                     /locus_tag="ACHGT5_000276"
     CDS             51151..51888
                     /locus_tag="ACHGT5_000276"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216296.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            51878..52600
                     /locus_tag="ACHGT5_000277"
     CDS             51878..52600
                     /locus_tag="ACHGT5_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN7216297.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(52684..>53103)
                     /locus_tag="ACHGT5_000278"
     CDS             complement(52684..>53103)
                     /locus_tag="ACHGT5_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN7216298.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        18306 a         9116 c        11118 g        14563 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     6001 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     6061 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     6121 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     6181 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     6241 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     6301 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     6361 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     6421 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     6481 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     6541 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     6601 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     6661 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     6721 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     6781 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     6841 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     6901 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     6961 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     7021 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     7081 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     7141 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     7201 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     7261 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     7321 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     7381 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     7441 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     7501 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     7561 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     7621 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     7681 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     7741 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     7801 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     7861 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     7921 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     7981 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     8041 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     8101 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     8161 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     8221 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     8281 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     8341 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     8401 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     8461 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     8521 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     8581 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     8641 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     8701 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     8761 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     8821 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     8881 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     8941 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     9001 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     9061 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     9121 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     9181 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     9241 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     9301 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     9361 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     9421 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     9481 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     9541 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     9601 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     9661 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     9721 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     9781 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     9841 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     9901 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     9961 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
    10021 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
    10081 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
    10141 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
    10201 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
    10261 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
    10321 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
    10381 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
    10441 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
    10501 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
    10561 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
    10621 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
    10681 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
    10741 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
    10801 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
    10861 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
    10921 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
    10981 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
    11041 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
    11101 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
    11161 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
    11221 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
    11281 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
    11341 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
    11401 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
    11461 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
    11521 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
    11581 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
    11641 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
    11701 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
    11761 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
    11821 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
    11881 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
    11941 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
    12001 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
    12061 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
    12121 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
    12181 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
    12241 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
    12301 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
    12361 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
    12421 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
    12481 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
    12541 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
    12601 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
    12661 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
    12721 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
    12781 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
    12841 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
    12901 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
    12961 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
    13021 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
    13081 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
    13141 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
    13201 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
    13261 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
    13321 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
    13381 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    13441 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    13501 tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    13561 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    13621 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    13681 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    13741 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    13801 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    13861 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    13921 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    13981 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    14041 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    14101 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    14161 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    14221 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    14281 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    14341 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    14401 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    14461 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    14521 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
    14581 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
    14641 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
    14701 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
    14761 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
    14821 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
    14881 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
    14941 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
    15001 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
    15061 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
    15121 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
    15181 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
    15241 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
    15301 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
    15361 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
    15421 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
    15481 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt
    15541 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
    15601 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
    15661 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
    15721 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
    15781 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
    15841 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
    15901 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
    15961 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
    16021 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
    16081 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
    16141 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
    16201 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
    16261 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
    16321 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
    16381 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
    16441 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
    16501 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
    16561 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
    16621 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
    16681 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
    16741 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
    16801 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
    16861 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
    16921 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
    16981 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
    17041 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
    17101 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
    17161 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
    17221 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
    17281 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
    17341 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
    17401 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
    17461 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
    17521 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
    17581 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
    17641 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
    17701 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
    17761 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
    17821 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt
    17881 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
    17941 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
    18001 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
    18061 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
    18121 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
    18181 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
    18241 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
    18301 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
    18361 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
    18421 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
    18481 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
    18541 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
    18601 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
    18661 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
    18721 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
    18781 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
    18841 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
    18901 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
    18961 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
    19021 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
    19081 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
    19141 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
    19201 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
    19261 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
    19321 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
    19381 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
    19441 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
    19501 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
    19561 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
    19621 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
    19681 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
    19741 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
    19801 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
    19861 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
    19921 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
    19981 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
    20041 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
    20101 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
    20161 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
    20221 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
    20281 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
    20341 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
    20401 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
    20461 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
    20521 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
    20581 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
    20641 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
    20701 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
    20761 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
    20821 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
    20881 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
    20941 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
    21001 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
    21061 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
    21121 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
    21181 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
    21241 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
    21301 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
    21361 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
    21421 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
    21481 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
    21541 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
    21601 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
    21661 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
    21721 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
    21781 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
    21841 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
    21901 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
    21961 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
    22021 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
    22081 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
    22141 agacggatag tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg
    22201 aggaattgcg ccaattgtga acaaacttag aaatattggt taaattctga caatttattt
    22261 gtctttttat aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga
    22321 cagaagccac gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag
    22381 aaaaaacgaa agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat
    22441 ctggagacgc tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat
    22501 tagctgatca tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac
    22561 gttcgtttta tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg
    22621 ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag
    22681 cactttttga cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg
    22741 aagtccttta cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca
    22801 ttgattttga ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc
    22861 ctctaggttc ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat
    22921 tgatgacaaa atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa
    22981 tcacgcctac tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata
    23041 gtgatatcca tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg
    23101 cagatttgcg gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct
    23161 atgcccatgt aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt
    23221 aaaaataagg aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt
    23281 caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag
    23341 taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca
    23401 atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa
    23461 aatttgaagg caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag
    23521 ctcaagaatg gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga
    23581 atgataaaga aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta
    23641 tcttagctat cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact
    23701 atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag
    23761 atatctgtat ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat
    23821 atgtatgaat taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta
    23881 ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg
    23941 cagttagatg aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct
    24001 acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt
    24061 gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca
    24121 atggttcggg atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt
    24181 gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt
    24241 atcaaaaaag aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat
    24301 gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat
    24361 cgccgtacag tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt
    24421 gaagttgaag accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg
    24481 atcgacctaa atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta
    24541 acagtcaaag aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat
    24601 gctgatatcc atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat
    24661 gaaatcgata agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc
    24721 caaagagaca tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc
    24781 caaacagatc acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat
    24841 ctaattccag aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct
    24901 gaagattttg tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg
    24961 ttgatcggta cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct
    25021 caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc
    25081 attttggaaa aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa
    25141 ataacgatca cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat
    25201 ttaagtcgct atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa
    25261 cccgcaagat aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc
    25321 cttacgataa aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag
    25381 gaagtgacgg agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca
    25441 aaacaatgtt tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga
    25501 tgaaaactga agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa
    25561 gagagaaata tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt
    25621 tgggtacgat tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag
    25681 cagaatatgg agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg
    25741 aagcagatgt acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg
    25801 aattgaaggc tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg
    25861 cttctaatat tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa
    25921 aattggaatc agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa
    25981 aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg
    26041 actggggcag atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg
    26101 gaggaatggc aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac
    26161 acggtgcgga attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag
    26221 gtgatccagc gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa
    26281 aagatgatca gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc
    26341 gggatatgga ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga
    26401 gtacgccgga gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat
    26461 taggaggaga cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt
    26521 tcttttctat cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg
    26581 aagatttcta tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc
    26641 cattcatcga tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc
    26701 gtgaactttt tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca
    26761 ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag
    26821 ggatctggtc tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa
    26881 tcgctgacac ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag
    26941 gtgcccatga aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg
    27001 agaagttagc agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata
    27061 agggagtgtg acatcgattt gtcacactcc cgttttatct ctttggaatg aaaaagtatc
    27121 aggatttttt cttgctgcta ttcaaaccaa acgggattcg actttcagta tggtttctga
    27181 tacgcttgat attgtcttta tgccgataaa agataaagga agaaactaaa aaggcaatga
    27241 tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc tacataagga aggacgatcg
    27301 tagataaagt gatcaatact gcactgatca tactgctcaa actcaccata cttgtacaaa
    27361 ataaactaac gataaagatg atacaagaat aaacaaaaaa tacaggatta aatcctaaaa
    27421 gcattcctgc acttgtcgca acagcttttc ctcctttgaa cccagcaaaa atcggaaatg
    27481 tatggccaag aatggcgcat accccaaacc aaagagggtt aatcccagaa aggtggaaga
    27541 tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc cataagcagg acaacaatcc
    27601 cggctgtttt gcctaataca cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt
    27661 gtcggatatc ttttttgaaa aacaatttgc caatccagac accggaagga atagaaccta
    27721 aaagatatgc taaaataagt aataagatca atttcatttt ttaccctctt tcaagttgaa
    27781 aactatcctt tattttagca tgaataggta acaggaacaa ttgaaaaaat gaaagataca
    27841 agtataatag atggaataaa aagaaaagca aggggtggca gaaatgtctg taaaaaatcc
    27901 aacacaagaa acgattttca actatttgga agaagcaaaa aatattgccg tcgtaggctt
    27961 gagcaataaa ccagaacgaa caagttacat ggttgcacag gtattgcaag acaatggata
    28021 taaaatcata ccagtaaatc cattattggc tggtgaagaa attctaggcg agaaagtcta
    28081 tgcgaatttg acagaaatcg atgatccaat cgatatcgta gatgtattta gacggagtga
    28141 atttttacct gaaatagcag gtgatttttt gaaaactgat gctaaagtgt tctgggcaca
    28201 attagggatt gaaaacaaag aagcagctga gatgctaaga aaagcaggac gtacagatat
    28261 cgtaatggat cgttgtatta agattgaact tggtaagttg aaagaaaacc aaaaataaaa
    28321 atgaccacaa agaagagatg atcgatagag gggaatgtcg aaaaagcggt taggagataa
    28381 ttaaaattga tttctcgtct accctctttt ttttcacgac cttttctagt atgataatcg
    28441 tggtgcggtt ttgccgcaaa gtattattga aggagcgttt gccttggcaa aaaaagtaaa
    28501 caacgaatac aatgactcgt ccatccaagt attggaagga ttagaggcag ttagaaaaag
    28561 accagggatg tacattggat ccactgacag ccgaggcttg catcatttag tctatgaaat
    28621 cgtcgataat gcagttgacg aagcattatc aggttatggc agtgagattg atgtgaccat
    28681 ccatgaagac aatagtataa ctgttgcaga tagcggacgt gggatgcctg taggaatgca
    28741 tgcttccgga ataccaacag ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt
    28801 tggtcaagga gggtacaaaa cttctggtgg actgcacggt gtaggagcca gtgtggtaaa
    28861 tgccttatcg aaatggctaa ttgtaacgat cgttagagat ggcgtagagt atcagcagaa
    28921 atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc
    28981 aaacgggacg actgtccatt ttttaccaga tgatacaata ttcagtacaa cgaaattttc
    29041 atatgaaatt ttagccgaac ggttaagaga atctgccttt cttctaaaag gcgttaaaat
    29101 ctctctttct gatcttcgtg gagaagaacc agtcaaggaa atttttcatt acgaagaagg
    29161 aatcaaagaa tttgttgatt atctgaatga agaaaaagat acattgacac cagttgttta
    29221 tttttcagga gaaaaagaag gtatcgaagt agaggtcgct tatcaataca atgacggtta
    29281 ttcagaaaat gttttatctt ttgtcaataa cgttcggaca aaagatgggg gaacccatga
    29341 agctggtatg aaagcggcga tgacgaaatc ttataatgaa tatgcgagaa aagttggttt
    29401 gctgaaagaa cgggacaaga atctggaagg tagtgatttc agagaagggt tagctgctgt
    29461 actttctatc cgtgtacctg aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact
    29521 tggtacgcca gttgctcgta ccgtagtaga taacgtcatc agtgaacaaa tgggctttta
    29581 tttgcaggaa aatagcgaaa tgagccagat gttggtccgt aaagcaatca aagccagaga
    29641 agcgagagaa gcagcacgta aagccagaga agaaagccga aatgggaaga agcgtaaaaa
    29701 aggcgaatct cttctttcag gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa
    29761 tgaattgtat ttagtcgaag gggattctgc tggcggatct gctaaacaag gaagagacag
    29821 aaagttccaa gcaattctac ctttacgagg aaaagtcatc aataccgaaa aagctaaaat
    29881 gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc tatacgattg gtgcaggagt
    29941 cggtcctgag ttttctatcg aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc
    30001 ggatacagac ggcgcccata ttcaagtatt actgcttact ttcttttatc gttatatgaa
    30061 accgctgatt gaagctggga aagtatatat cgcgttaccg cctttatata aagtatccaa
    30121 aggacaagga aagaagcaag tcattgaata tgcgtggaca gatgacgaat tggcagcaat
    30181 gattaaaaaa gtcggcaaag gatatatgct gcagcgatat aaaggacttg gagagatgaa
    30241 tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc cgtacgttga tccgcgtccg
    30301 tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga
    30361 acctcgaaga aaatggatcg aaaatcacgt gcaatttacg ctcgaagaag atggcagtat
    30421 cttagataaa aaagaggata ctgaaatctc tccttcagtt tctaatgatt tattagatga
    30481 agaacgagca gataaaaatg aaaacaatca gttatttgaa gtagaatagg aggtcacctg
    30541 atggaaaatc gacaagaagt acaagaatta actttagaag aagtgatggg tgaccgcttc
    30601 ggacgttatt ccaaatatat tatccaagaa cgtgcactgc ctgatatccg tgatggattg
    30661 aagccagtgc aacgccggat attatttgcc atgaacaaag atggcaatac gtatgacaaa
    30721 ggattccgta aatcagcaaa atctgtcgga aatatcatgg ggaattatca tcctcatggt
    30781 gacatcagta tttatgaagc gatggtccgc atgagccaag actggaagtt gcgggaagta
    30841 ctgatcgaaa tgcacgggaa taacgggagt atggacggtg atccacctgc tgcgatgcgt
    30901 tatactgaag ccagattatc caagttaagt ggagagatgc tagcagatat cgagaaagaa
    30961 acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag aaccaacagt ccttccagct
    31021 cgatacccaa accttctcgt caatggatcg acaggtatat ctgctggata tgcaacagaa
    31081 atcccgactc ataacttagc tgaagtgatc gatggcacaa tctatatgat cgatcatcca
    31141 caagcgagcc ttgaaaaatt gatggaatat attcctggac ctgactttcc aacaggaggg
    31201 atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt
    31261 cttcgttcga aaacaaagat agaggcaatg aagggtaata aacagcaaat cgtgatcaca
    31321 gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa aaatggatga gatccgtttg
    31381 aataagaaaa tcgatggaat agctgaagta cgagatgaaa gtgatagaac aggtctgcaa
    31441 attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa ttttgaatta tttgttcaaa
    31501 aacactgagc tacagatcaa ttacaatttc aatatggtcg ctatcgacca tatgactccg
    31561 catcaagttg gactgaaaga cattttaaga agttatatcg agcatcgaaa gcaagtcata
    31621 acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa gacagcatat cgtagaagga
    31681 ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac
    31741 aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat ttacagaaga acaagcagaa
    31801 gcaattgtca cattgcaatt ataccgtttg acgaacacgg atatcacaga gcttcaaaag
    31861 gaatccgaaa gcttgattgc gcaaatcaca gagttgaata aaatcctttc aaatgacaaa
    31921 gagcttttct ctgttatgaa aaaagaattg cgagaagtga aaaaaaatta ttcttccgct
    31981 cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg
    32041 gcacaagaag acgtaattgt cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt
    32101 cgttcttata gtgcttccaa accagaagaa atcgggatgc gagaaggcga ctacttgcta
    32161 tactcaggtg aattgagtac attggaccat gttcttctga tcacaaataa agcaaatgtg
    32221 atctaccgcc ctgtccatga actacccgat ttgaaatgga aagatgctgg tgagcacatt
    32281 tctcaaacaa tctccaatct gtctgtagac gaatcgattt tagctgtttt cccatatcaa
    32341 aaaattcaag cagagaaaac atttgtattc atcagcaaga atggattgat caagcaaaca
    32401 cgaatgacag agttcgaacc atggcgcacc tataagagcc gtccgctcag ctgtatgaag
    32461 ctaaaggcgg aagaagatga actgattgct gtgtatctga aagaagatca gccaggctta
    32521 gatgttttct tagtaacgca tcaaggaatg ggtttgcgct atcctttaac agatgttccc
    32581 gttgttggag caaaggcagc tggagtgaag tctatgaatt tgaaagatgg agataggatt
    32641 atcaacgggc tgcttgtgtt agcagaaggt gatacgccga tcgtaatcgt tactcagcga
    32701 ggagctgtta aacgcatgct tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga
    32761 gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga
    32821 agatcaaaag aaataacctt gatgaatcaa aaagggcagc agttaacgat tgacccaact
    32881 gatttcccta taggtgatcg aacatcgaat ggatcatttg cgatggatga gaaaaaaggt
    32941 ggagaagtca tcgaagttat cgatgctcct gagattttca tcgagtcgta actgttgtag
    33001 tattttaatg aagagacgcc atctgtatta atacaggtgg cgtctctttt tcattcattt
    33061 ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg
    33121 gtatgtcaac gttgatttaa atatatgtaa ataaaacata aaaattgtta aataaaaaac
    33181 aagaaaaccc ttgcaaagat agggtgctgt gttatactaa tcacgtaaaa actgttacat
    33241 aaaacagaaa tttaggaggt ccatacaatg gagcaatgga atggatttaa aggtaccaaa
    33301 tggaaagaag gcgtagacac tagagacttt attcaaaata actacactga atatcgcggt
    33361 gatgacagct ttttggaagg cccaacagaa gcaacagaaa aattatggga tgaattgcaa
    33421 aagttaaatg atatccaatt cgagcgtaac ggtgtctatg atatggacac agaaatcgta
    33481 tcaactatta cttcacacga tgcaggctat ttggataaag atttggaaaa aatcgtcggt
    33541 ctacagacag acaaaccttt gaaacaagca ttcatgccgt tcggcggaat caatatggct
    33601 aacaatgcgc taaaaagcaa tggctataca ccaaacgatg aattgacaca tattttcact
    33661 gactggcgca aaacacataa tcaaggtgta tttgacgctt atactgctga aatgcgtgct
    33721 gctcgtaaaa acaaaatcat tacagggctt ccagacgctt acggtcgcgg acgtatcatc
    33781 ggtgactatc gtcgtgtggc attgtatgga atcgactatt tgatggaaca aaagaaaaaa
    33841 gatcatgata tgactggtta tcatacaatg agtgaagaag tgattcgttt acgtgaagaa
    33901 atcactgaac aatatcgtgc attagcgcaa atgaaagaaa tggctgcgaa atatggctat
    33961 gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc aatggctata cttcggatat
    34021 ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc
    34081 cttgatatct atatccaacg tgatttggat aatggagtaa tcacagaaaa agaagcacaa
    34141 gaattgatcg accacttagt aatgaaatta cgtatggtca aattcgcgcg tacacctgaa
    34201 tataaccaat tattctcagg attccctatc tgggctactt tatccattgc aggtatggga
    34261 ttagatggtc gttcattagt tacaaagaat gatttccgtc tgcttcatac attaacaaat
    34321 atgggacctt caccagaacc aaacttgact gttttgtatt ctgaacacct tccagaagga
    34381 ttcagaacat atgctagcag aatcgctatc gaaagttctt cgattcaatt cgaaaatgat
    34441 aatcagcttc gtgaacaatg gggttctgat gattgtgcga ttgcttgctg tgtatcagga
    34501 acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg ctaacttggc taaaacacta
    34561 ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg cccaagttgg accagaatat
    34621 cgtccaatca catcagaaat actagacttt gaagaagtga aacataactt catgaatatg
    34681 atggattggt tagcagaact atatgtcaat acgttgaaca tcattcatta tatgcatgat
    34741 aaatattctt atgaagcagc tcagctggct ttgatggatt ctgacttgca acgcacattt
    34801 gctacaggta ttgctgggat ctctcatgcg gcagacagct tgtcagcgat caaatatgca
    34861 aaagtaaaag caatccgtaa tgaaaaagga atagcagttg attttgaagt ggatggcgac
    34921 ttccctaaat acggaaatga tgatgaccgt gcagatgaat tagtttcatg gatcttggaa
    34981 tacttcatgg gacaaatcaa acgtcataaa acttaccgca actcaaaacc aacaacttca
    35041 ttattgacga ttacttcaaa tgttgtttac ggtaaaaata caggaaatac tcctgatggc
    35101 cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt
    35161 gggttattag ctagcctgaa ctcaacagct aaaatgaatt acggctgtgc attggatgga
    35221 atttcaaata cccaaacaat caatccttca ggtcttggaa aagaacaaga tacaagaatc
    35281 gaaaacttgc gtaatgtctt agatggatac ttctcaaaag gagcttatca tttgaatgtc
    35341 aatgtattca gtaatgattt attgcgtgat gcaatggaaa atccaatgaa atatccgaac
    35401 ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg acttgacaag agaacaacaa
    35461 ttagatgtct tgatgagaac ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt
    35521 tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg
    35581 cttatttgaa agaaacgatt tacaggggca ggacttgtat gttccgtccc tgtaaatttt
    35641 acacataacg aagcggcaat tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta
    35701 acttgtactt aaaatgaaat caaagaaaag atgtgaactt aaatggaaga aaaaacaatc
    35761 ggctatgttc attctatcga aactttcggt tcagtagacg gacctggact gcgctttgtc
    35821 gtgtttatgc aaggatgccg catgcgttgc caattttgtc acaatccaga tacttggaat
    35881 attggcggag ggaaagaata cacagcagat gaacttttag ataaagcaga acgcttcaga
    35941 ccttactggg gagataaagg tggtattact gttagtggtg gagaacctct attgcagatc
    36001 gattttttga tcgaactatt taaaaaagca aaagagcgga agatgcatac tacattggat
    36061 acatgcggaa aaccttttac gtatgaagaa cctttctttt cacgttttca agaattgatg
    36121 aagtatacag atttactgtt atttgacatc aaacacatcg ataatgaaga gcataaaaaa
    36181 ctaacgcatt gggacaatga aaatatttta gaaatggctc aatatttgtc aaaaatcaac
    36241 aaaccggttt ggattcgcca tgtactagtt cctgagcgaa gtgattatga tgaatatttg
    36301 atccgactag acaactttat tcaaaccttg tcaaatgtag acagagtaga gattcttcct
    36361 tatcatacga tgggaaaata caagtgggaa acacttggtt tgaaatatcc attagaaggg
    36421 atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac gattgcttca tacagaagag
    36481 tatacaaaat atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc
    36541 gtttttttgc tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt
    36601 ttagtttttg gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca
    36661 cacttgcaaa atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa
    36721 gaaacacaat acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct
    36781 aaagcagaaa atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca
    36841 gatatcagcg aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa
    36901 acagctaatc cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta
    36961 aaactatata aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca
    37021 gctatcattt cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca
    37081 gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg
    37141 ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca
    37201 aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt
    37261 aatgaagtga tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca
    37321 aatggctacg atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta
    37381 cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac
    37441 aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg
    37501 gctgtattta gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg
    37561 atataaaaag aaacatagaa catactaaag gccgaagaaa tggaggagaa taacagtcaa
    37621 ttatgactct tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat
    37681 agaaaagaga acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact
    37741 gaccgatgat ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga
    37801 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
    37861 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
    37921 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
    37981 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
    38041 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
    38101 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
    38161 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
    38221 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
    38281 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
    38341 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
    38401 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
    38461 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
    38521 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
    38581 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
    38641 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
    38701 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
    38761 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
    38821 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
    38881 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
    38941 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
    39001 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
    39061 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
    39121 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
    39181 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
    39241 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
    39301 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
    39361 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
    39421 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
    39481 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
    39541 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct
    39601 acgttgaatt tccataatat ctccaaatta aattgataag tatataaagt ataataacaa
    39661 attgatgagt atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt
    39721 taaaatttaa tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt
    39781 tctcactttt ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa
    39841 agcacgctct tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga
    39901 aagtatttcg agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc
    39961 ataaccgtgt tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa
    40021 aatcgttgtg accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact
    40081 gatacctact gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc
    40141 taaaacaagg gataagccga cgatataaat cgtgatctcg accatactga caagtggagc
    40201 aatcagtcga tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc
    40261 aaaattaatg aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt
    40321 catttatata acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac
    40381 atacaaaaaa atgataaaat gattataata gaattataaa gataggttag gtgattaaaa
    40441 tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat
    40501 acttaggtgt ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt
    40561 cgctgacaga tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata
    40621 ttttcggtag cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat
    40681 taggagtggt gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta
    40741 tattatttgg tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat
    40801 caggaaattt aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg
    40861 tccatcatat tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg
    40921 ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa
    40981 aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca
    41041 aacagccggt ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt
    41101 ttgaatttga cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg
    41161 cagttggcgg ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc
    41221 atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa
    41281 tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt
    41341 catgggttaa gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt
    41401 tcttttggca tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag
    41461 aaaaaaatcg gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc
    41521 gacatatggt acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga
    41581 taaaaaagta taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt
    41641 tttattttgg gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg
    41701 acaacatgtt agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa
    41761 tgtttcaaaa atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt
    41821 gaaagagtta gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa
    41881 agcacttgtc acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac
    41941 aactgagccc tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca
    42001 ttattctctc caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat
    42061 catcacaggt gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt
    42121 cttgtttggg gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac
    42181 agcaaaagca acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat
    42241 cgaagtggac gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga
    42301 agttgatctt cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata
    42361 tatgacagaa aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg
    42421 gctagcacta ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt
    42481 tggtgtgatt ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac
    42541 gatcaagcaa gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat
    42601 agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga
    42661 gacaacgaat taagtcttat tactatatat atatttatga aattcatcgc tattttctta
    42721 aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc
    42781 atttttgaga aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc
    42841 gtacgatgat gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg
    42901 acaagaagat cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca
    42961 tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa
    43021 gtcagctccg cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg
    43081 ccattaccaa tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg
    43141 ttctgtcaga caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt
    43201 tgacacgaag gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat
    43261 cccagaccct agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac
    43321 ggatgactct tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg
    43381 tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga
    43441 agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga
    43501 tgatcaagga aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat
    43561 attcggctac agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa
    43621 agtttatatc gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta
    43681 agaaaataat ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt
    43741 tattttttta taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt
    43801 ctgtaaatta aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc
    43861 aagtggttca aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat
    43921 ttccagatac ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata
    43981 cagaaaatgt gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa
    44041 gaaaagactt ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc
    44101 tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat
    44161 acctgtcatc aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc
    44221 ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg
    44281 ttttccaaaa agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac
    44341 agaaggagcg aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc
    44401 tgccagctta gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa
    44461 aatgattccg cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt
    44521 tgaaaatttt cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat
    44581 gaaaaaaaat tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg
    44641 agcagcagac ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt
    44701 ttttttggct caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa
    44761 tcaatttatc tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct
    44821 ttttttgatg aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc
    44881 tctttatgga gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca
    44941 agacgtttat gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata
    45001 cagtataata ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt
    45061 attggctgtt tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc
    45121 caagtgacaa tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa
    45181 gttgatccga ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt
    45241 cttttttttg ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga
    45301 aatgtttata cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat
    45361 taagtgaaag aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca
    45421 tttctttgct gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata
    45481 aaaaaatggc agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg
    45541 ccttattagg tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac
    45601 ttgttattta cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg
    45661 acccgtctat tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga
    45721 aagtagagct tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt
    45781 taatcatcgg tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta
    45841 ttttgttagg tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg
    45901 ccttgcttgc aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat
    45961 attccatcat tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt
    46021 ctgccttgtt taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa
    46081 tcctgatcca aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa
    46141 aaccgaattt tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata
    46201 tctatcctga gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga
    46261 aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc
    46321 aattcgatat gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca
    46381 agagatcttt tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg
    46441 atcagatcaa agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa
    46501 tccaagaagc atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt
    46561 accaagcaat caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc
    46621 tcaaggagta cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag
    46681 gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga
    46741 ataaattaag cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg
    46801 tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa
    46861 aggaggaaga tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg
    46921 aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt
    46981 tggtccttcc ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc
    47041 atcagcagga gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat
    47101 gcggtggaat atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa
    47161 gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg
    47221 tgtagatgaa cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc
    47281 tagagagttg tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag
    47341 cccagatgtg attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc
    47401 attacaagaa ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac
    47461 gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga
    47521 aattatccaa tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg
    47581 gcatttcttt gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa
    47641 tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc
    47701 tatgaaagaa gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca
    47761 aagaagtatt gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga
    47821 aggaaatgaa aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg
    47881 cttatggatt gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg
    47941 ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac
    48001 gtaatcgggt aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg
    48061 aagaacttca aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac
    48121 aattagcaga agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat
    48181 ctattgaagt caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa
    48241 gacctacgat cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat
    48301 cattgcctgt actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc
    48361 tagctacgat tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc
    48421 cattaaaaga atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg
    48481 gcattcagtt tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc
    48541 gtttagtcgg aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac
    48601 agccgaatac agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa
    48661 ttgcagtcaa tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg
    48721 tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta
    48781 agcggataac ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct
    48841 acgccagtcc taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc
    48901 aagtggtaga gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg
    48961 ctaaggctga attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg
    49021 agcaagcaga cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa
    49081 aagagttgga tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat
    49141 tgaaataatc agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt
    49201 aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt
    49261 agacccattg aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag
    49321 cgggttacta tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag
    49381 gtaacaaaat gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt
    49441 ggcagattgt tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg
    49501 tattttctgc attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa
    49561 atgaagcagt gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa
    49621 tggattattg gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt
    49681 tgccaatcat ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa
    49741 aagtgatcct tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat
    49801 tgaggaaagt atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga
    49861 agatttatcg aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag
    49921 tggctgctac ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag
    49981 gcttagggta ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg
    50041 cgatcatttg tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa
    50101 aagggtatta tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg
    50161 gcttttatta ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag
    50221 caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac
    50281 taatcatact ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa
    50341 tgtccaaatc atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa
    50401 agcacaattt ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa
    50461 tataccagtc aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct
    50521 atcagacagt aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca
    50581 aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc
    50641 aaaactgaca atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga
    50701 tatccaatgg tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact
    50761 aaactatatc ccattgaaag aattagatga acggttagat tactatactc cggtaattat
    50821 tacgaatgat caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac
    50881 gaaaaaaggc tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa
    50941 atatgcaaaa gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa
    51001 ttatacggat gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac
    51061 agcttttatg caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac
    51121 taatcagttt ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta
    51181 gtcattgaaa acctgaaaat attagaagac atccatttca ctatccctaa agataccttt
    51241 acatcgattg tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga
    51301 gtccagccta tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat
    51361 acagcattta gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa
    51421 aacgttacgt tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat
    51481 tatctggata tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc
    51541 gggatgaagc agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt
    51601 cttgatgaac catttggagc gttagatgct atgaccagag ggcagatgca agactggatg
    51661 ttgaagctgc ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa
    51721 gcaatttatt tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct
    51781 gagattcaag tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg
    51841 aagcaaacga tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca
    51901 tttagatggg agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat
    51961 caattgtgat tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca
    52021 agccttaagt tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga
    52081 gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa
    52141 tgtaccgatg actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa
    52201 tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca
    52261 agcttaccct aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta
    52321 tattgattta ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg
    52381 gcaactagct aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc
    52441 agcagagtgg gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga
    52501 acagttaaag acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac
    52561 ttatcaaaac ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact
    52621 agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga
    52681 atcttagtac caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg
    52741 tgaagtaaca tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa
    52801 gtaagaagaa tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc
    52861 gcttgattct ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc
    52921 agtttctgtt acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt
    52981 ttgaaccgtt tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc
    53041 ttgaggttca gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga
    53101 gta
//
LOCUS       DAZBWR010000005        53086 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000005 DAZBWR010000000
VERSION     DAZBWR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.005"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..3006
                     /locus_tag="ACHGT5_000279"
     CDS             199..3006
                     /locus_tag="ACHGT5_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN7216299.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3197..3688
                     /locus_tag="ACHGT5_000280"
     CDS             3197..3688
                     /locus_tag="ACHGT5_000280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN7216300.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3791..4780
                     /locus_tag="ACHGT5_000281"
     CDS             3791..4780
                     /locus_tag="ACHGT5_000281"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN7216301.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4813..5616
                     /locus_tag="ACHGT5_000282"
     CDS             4813..5616
                     /locus_tag="ACHGT5_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN7216302.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5637..6551
                     /locus_tag="ACHGT5_000283"
     CDS             5637..6551
                     /locus_tag="ACHGT5_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN7216303.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6634..7008
                     /locus_tag="ACHGT5_000284"
     CDS             6634..7008
                     /locus_tag="ACHGT5_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN7216304.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7147..7392
                     /locus_tag="ACHGT5_000285"
     CDS             7147..7392
                     /locus_tag="ACHGT5_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN7216305.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7564..8628
                     /locus_tag="ACHGT5_000286"
     CDS             7564..8628
                     /locus_tag="ACHGT5_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN7216306.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            8874..9299
                     /locus_tag="ACHGT5_000287"
     CDS             8874..9299
                     /locus_tag="ACHGT5_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN7216307.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9292..9741
                     /gene="nusB"
                     /locus_tag="ACHGT5_000288"
     CDS             9292..9741
                     /gene="nusB"
                     /locus_tag="ACHGT5_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN7216308.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9838..10692
                     /locus_tag="ACHGT5_000289"
     CDS             9838..10692
                     /locus_tag="ACHGT5_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN7216309.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10693..12030
                     /gene="xseA"
                     /locus_tag="ACHGT5_000290"
     CDS             10693..12030
                     /gene="xseA"
                     /locus_tag="ACHGT5_000290"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN7216310.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12035..12259
                     /locus_tag="ACHGT5_000291"
     CDS             12035..12259
                     /locus_tag="ACHGT5_000291"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN7216311.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12261..13151
                     /locus_tag="ACHGT5_000292"
     CDS             12261..13151
                     /locus_tag="ACHGT5_000292"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN7216312.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13160..13978
                     /locus_tag="ACHGT5_000293"
     CDS             13160..13978
                     /locus_tag="ACHGT5_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN7216313.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14085..14534
                     /locus_tag="ACHGT5_000294"
     CDS             14085..14534
                     /locus_tag="ACHGT5_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN7216314.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14552..16234
                     /gene="recN"
                     /locus_tag="ACHGT5_000295"
     CDS             14552..16234
                     /gene="recN"
                     /locus_tag="ACHGT5_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN7216315.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16289..16405)
                     /locus_tag="ACHGT5_000296"
     CDS             complement(16289..16405)
                     /locus_tag="ACHGT5_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN7216316.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16478..17434)
                     /locus_tag="ACHGT5_000297"
     CDS             complement(16478..17434)
                     /locus_tag="ACHGT5_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN7216317.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17605..17973
                     /locus_tag="ACHGT5_000298"
     CDS             17605..17973
                     /locus_tag="ACHGT5_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN7216318.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18143..18574
                     /gene="mraZ"
                     /locus_tag="ACHGT5_000299"
     CDS             18143..18574
                     /gene="mraZ"
                     /locus_tag="ACHGT5_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN7216319.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18595..19554
                     /gene="rsmH"
                     /locus_tag="ACHGT5_000300"
     CDS             18595..19554
                     /gene="rsmH"
                     /locus_tag="ACHGT5_000300"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN7216320.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19559..19960
                     /gene="ftsL"
                     /locus_tag="ACHGT5_000301"
     CDS             19559..19960
                     /gene="ftsL"
                     /locus_tag="ACHGT5_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN7216321.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            19961..22153
                     /locus_tag="ACHGT5_000302"
     CDS             19961..22153
                     /locus_tag="ACHGT5_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN7216322.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22179..23141
                     /gene="mraY"
                     /locus_tag="ACHGT5_000303"
     CDS             22179..23141
                     /gene="mraY"
                     /locus_tag="ACHGT5_000303"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN7216323.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23155..24516
                     /gene="murD"
                     /locus_tag="ACHGT5_000304"
     CDS             23155..24516
                     /gene="murD"
                     /locus_tag="ACHGT5_000304"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN7216324.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24542..25630
                     /gene="murG"
                     /locus_tag="ACHGT5_000305"
     CDS             24542..25630
                     /gene="murG"
                     /locus_tag="ACHGT5_000305"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN7216325.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25645..26865
                     /locus_tag="ACHGT5_000306"
     CDS             25645..26865
                     /locus_tag="ACHGT5_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN7216326.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27021..28346
                     /gene="ftsA"
                     /locus_tag="ACHGT5_000307"
     CDS             27021..28346
                     /gene="ftsA"
                     /locus_tag="ACHGT5_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN7216327.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28369..29610
                     /gene="ftsZ"
                     /locus_tag="ACHGT5_000308"
     CDS             28369..29610
                     /gene="ftsZ"
                     /locus_tag="ACHGT5_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN7216328.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29627..30304
                     /locus_tag="ACHGT5_000309"
     CDS             29627..30304
                     /locus_tag="ACHGT5_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN7216329.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30318..30917
                     /locus_tag="ACHGT5_000310"
     CDS             30318..30917
                     /locus_tag="ACHGT5_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN7216330.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            30998..31219
                     /locus_tag="ACHGT5_000311"
     CDS             30998..31219
                     /locus_tag="ACHGT5_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN7216331.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31268..32050
                     /locus_tag="ACHGT5_000312"
     CDS             31268..32050
                     /locus_tag="ACHGT5_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN7216332.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32071..32778
                     /locus_tag="ACHGT5_000313"
     CDS             32071..32778
                     /locus_tag="ACHGT5_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN7216333.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33040..35838
                     /gene="ileS"
                     /locus_tag="ACHGT5_000314"
     CDS             33040..35838
                     /gene="ileS"
                     /locus_tag="ACHGT5_000314"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN7216334.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(35887..37413)
                     /gene="zwf"
                     /locus_tag="ACHGT5_000315"
     CDS             complement(35887..37413)
                     /gene="zwf"
                     /locus_tag="ACHGT5_000315"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN7216335.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37428..38075)
                     /locus_tag="ACHGT5_000316"
     CDS             complement(37428..38075)
                     /locus_tag="ACHGT5_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN7216336.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38259..38588)
                     /locus_tag="ACHGT5_000317"
     CDS             complement(38259..38588)
                     /locus_tag="ACHGT5_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN7216337.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38765..39493
                     /gene="sfsA"
                     /locus_tag="ACHGT5_000318"
     CDS             38765..39493
                     /gene="sfsA"
                     /locus_tag="ACHGT5_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN7216338.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39509..40522
                     /locus_tag="ACHGT5_000319"
     CDS             39509..40522
                     /locus_tag="ACHGT5_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN7216339.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40522..41799
                     /locus_tag="ACHGT5_000320"
     CDS             40522..41799
                     /locus_tag="ACHGT5_000320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN7216340.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            41862..44564
                     /locus_tag="ACHGT5_000321"
     CDS             41862..44564
                     /locus_tag="ACHGT5_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN7216341.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44716..45033
                     /locus_tag="ACHGT5_000322"
     CDS             44716..45033
                     /locus_tag="ACHGT5_000322"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7216342.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45063..45383
                     /locus_tag="ACHGT5_000323"
     CDS             45063..45383
                     /locus_tag="ACHGT5_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN7216343.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45491..46951
                     /locus_tag="ACHGT5_000324"
     CDS             45491..46951
                     /locus_tag="ACHGT5_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN7216344.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47019..47240
                     /locus_tag="ACHGT5_000325"
     CDS             47019..47240
                     /locus_tag="ACHGT5_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216345.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47271..47453
                     /locus_tag="ACHGT5_000326"
     CDS             47271..47453
                     /locus_tag="ACHGT5_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFN7216346.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47453..47866
                     /locus_tag="ACHGT5_000327"
     CDS             47453..47866
                     /locus_tag="ACHGT5_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN7216347.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            47989..49170
                     /locus_tag="ACHGT5_000328"
     CDS             47989..49170
                     /locus_tag="ACHGT5_000328"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216348.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49241..49405)
                     /locus_tag="ACHGT5_000329"
     CDS             complement(49241..49405)
                     /locus_tag="ACHGT5_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216349.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49559..49632
                     /locus_tag="ACHGT5_000330"
     tRNA            49559..49632
                     /locus_tag="ACHGT5_000330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49593..49595,aa:Arg,seq:cct)
     gene            complement(49701..50840)
                     /locus_tag="ACHGT5_000331"
     CDS             complement(49701..50840)
                     /locus_tag="ACHGT5_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7216350.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            51012..51146
                     /locus_tag="ACHGT5_000332"
     CDS             51012..51146
                     /locus_tag="ACHGT5_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216351.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(51139..51774)
                     /locus_tag="ACHGT5_000333"
     CDS             complement(51139..51774)
                     /locus_tag="ACHGT5_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216352.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(51887..52522)
                     /locus_tag="ACHGT5_000334"
     CDS             complement(51887..52522)
                     /locus_tag="ACHGT5_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFN7216353.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52556..53017)
                     /locus_tag="ACHGT5_000335"
     CDS             complement(52556..53017)
                     /locus_tag="ACHGT5_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFN7216354.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        17517 a         8980 c        11232 g        15357 t
ORIGIN      
        1 cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc tatctatccg
       61 cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata aatacatagt
      121 attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc attggcaaaa
      181 cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa
      241 tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca gcgaacaaat
      301 agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac agacggacga
      361 ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac aaaacatgta
      421 gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat agatactaag
      481 gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt ggaacaggca
      541 aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg acctaccgga
      601 tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca tcatgtaata
      661 gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa ccctgagctt
      721 ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa
      781 atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat tcaccggttg
      841 ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta cagtcgtctt
      901 ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac cacagaagac
      961 ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat caaacttccg
     1021 gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat gattgcccac
     1081 gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa agcattgatc
     1141 ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt agtatgtgca
     1201 cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga tgatttgaca
     1261 gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat ggcagagctt
     1321 tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat gaaaatccaa
     1381 tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa agcagcttta
     1441 ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac agatatcaac
     1501 atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga cagcaaatta
     1561 gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt aatggtcaaa
     1621 agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt gcatatcagt
     1681 tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa catccgtgag
     1741 atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa
     1801 acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt tctactcgtc
     1861 tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc tcacggaaac
     1921 agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga gaacgtacga
     1981 gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat tgaacgcaat
     2041 gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg gtcccttgcg
     2101 tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt tgatatggta
     2161 acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac agatctagat
     2221 atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc tgtagaatct
     2281 gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg cgcatctggc
     2341 gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa
     2401 acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact gccagttatc
     2461 caaaaagaat atcagatcat agctactact gggattactg atttgaatat ccaagcccca
     2521 tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga tcagttgctg
     2581 atggaatcag aattggagga aactgaattc atctccctag atgaagagtc agctaaaaat
     2641 acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa gttaatcgat
     2701 ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga tgaagaatat
     2761 ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat tattcggaat
     2821 gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc tctttatagc
     2881 caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt gccgatagaa
     2941 gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga atacactgtg
     3001 ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta ttggttaaga
     3061 atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc ttatagtagt
     3121 gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa gtagtgagga
     3181 agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt taatccacgg
     3241 gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga
     3301 tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac cgccggggat
     3361 cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg gattattatc
     3421 acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg ttgtccgctg
     3481 cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag gcaaatcaat
     3541 gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt acttgaacag
     3601 tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga
     3661 actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg tcaaataatt
     3721 ctgttgtaat ggttagcaat tattaatatt tttgaatttg ttacaacaaa agtaaatagg
     3781 aggtataaat atggtaggga ttatcctagc aagtcatgga caatttgctg aaggaatctt
     3841 acaatccggt tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc
     3901 aagtgaaggt ccagatgact tgagagccaa attggaagaa gcggttgctt cttttgataa
     3961 tcaagacgaa gtgttattct tagttgattt atggggcgga acaccgttca atcaatcaaa
     4021 cacattattc gaagagcaca aagataaatg ggcaatcgtc agcggtttga atttaccaat
     4081 gttgattgaa gcctacgcat cacgcttttc aatggaatct gctcatgaaa ttgcagcaca
     4141 catcattgaa acagcaaaag acggtgtaaa agtaaaaccg gaagaattag aaccggcaga
     4201 agcaccgaaa gcagcagttg aagacgcaca acctaaagga gcacttccag aaggaactgt
     4261 ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc tgttacatgg
     4321 acaagttgcg acagcttgga caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga
     4381 tgcagtatca aaggatgatc tgcgtaagag actaatcgaa caagctgctc ctccaggtgt
     4441 aaaagcaaat gttattccta ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg
     4501 aaataccaaa gcattgcttt tatttgaaaa tcctgaagac gtattgacag ctgttgaagg
     4561 tggcgtagac atcaaagaat taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt
     4621 tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa gcgtttgaga agcttgaaca
     4681 aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat tcacgcgata acatggatga
     4741 tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa taaataatat taaaatttat
     4801 aggaggctta tcatgtctat tatttcaata attttagtcg tattagttgc ctttctagct
     4861 ggtatggaag gtattcttga tgaatttcaa tttcatcaac cactagtggc atgtacattg
     4921 atcggtttag ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac gcttcagatg
     4981 atcgctttag gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc attagcatca
     5041 gtagcatcag caattatttt agttttaggt ggacaaggtg ttaaaggtgt tccttctgcg
     5101 atcgcgattg ccgttccttt ggctgttgca gggttgttct taacaatgat cgtacgtaca
     5161 atcgctgttc caatcgttca tttgatggat gcggctgctg aagaaggaaa catccgtaag
     5221 gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc ggtcccagca
     5281 gcagcgttgc tatttatccc agcacaaact gtgcaatcat tccttgaatc aatgccagca
     5341 tggttaacag atggtatggc tatcggtggg ggaatggtcg tagctgtagg ttacgctttg
     5401 gtaatcaaca tgatggctac aaaagaagta tggccattct tcgttattgg tttcgttgta
     5461 gctgcaattt ctcaattaac attgattgct ctaggtgctt taggtgttgc attagctctt
     5521 atttacttga acctttctaa aatgggtggt tcatcaaata atggaggcgg aagcaattct
     5581 ggtgaccctt taggcgacat tttgaatgac tattaatctt gaaggaggaa aataaaatgg
     5641 cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg cgctctacct
     5701 tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg tggctgggca ttttctatga
     5761 ttccagcaat caaaaaatta tacaaaacaa aagaagaacg ttctgcagca ttaaaacgtc
     5821 acttggaatt ctttaataca cacccataca ttgcttctcc aattttaggg gttacattgg
     5881 ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt caaggggtta
     5941 aagtaggtat gatgggtcct ttagctggtg ttggtgaccc agtgttctgg tttacagtac
     6001 gtccaatgct aggtgcacta ggtgcttcat tagctatggg aggaaatatc cttggaccaa
     6061 tcatcttctt cttaggttgg aacattattc gttgggcatt catgtggtat acacaagaat
     6121 ttggctacaa agctggttct aaaattactg atgacctttc tggcggattg ttacaagacg
     6181 taacaaaagg tgcttcaatt ttagggatgt tcgtattagc tgcccttgta caaagatggg
     6241 tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt agataaaggt gcttacatcg
     6301 aatgggataa attgccttca ggtggtgaag gaatcaagag tgcatttgaa caagttaata
     6361 atggcttagc cctttctcct gaaaaggtaa caacattaca agacaactta gaccaattga
     6421 ttcctggttt agctgcatta ggattaacat tgttatgtat gtggttgctt aagaaaaagg
     6481 tttctccaat cgttatcatc cttggtttat tcgtagtcgg tgttgttgga cacgtcatcg
     6541 gacttttata atccataaaa atatagctcg ggcgttgtcc cgagcttttt ttatatagta
     6601 tactgttcat aaacaaatat ctggagggag aaaatggttc aatcaatcaa tacaaaagta
     6661 gacttggtaa ttgatgctac ttcgtttacg ggtctgacag attacgggca aatcatgata
     6721 ggtaataatg gttttgagtt ttaccatgcg cgagatagac gcaaatatat ccaaattcca
     6781 tgggaagaag tggattatgt actagcatca gtcatgctaa agggaaaatg gattcctcga
     6841 tatgccattc aaacgaaaaa aaatggtaca tacacatttt cttccaaaga acctaaaaaa
     6901 gtgcttcgtg cgatcagaga atatgtagat gaagatagaa tggtacaatc attgagtttc
     6961 tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa aataaagaaa
     7021 acttctaaga ggaggttttc tttatttttt tactttgcag aagaaaatat gaaatagcaa
     7081 aaccaattat ttgtcccctt taaatcccat atgctaaaat tgattagcaa agaaggaggt
     7141 tatattatga gttggttatg gacattgatt gttggagccg tgatcggagc aattgcaggt
     7201 gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc tggactagtc
     7261 ggttcaagta ttggacaagc gattttcggc gaatggggac caaaagctgc aggtatggca
     7321 cttattccat ctattttagg cgcagtgatt ttggtagcag ttgtctcatt tttctttgga
     7381 aaacgatcat aagtttttac aaaatagtaa actaaaagga gttgcagcga gactgtgact
     7441 cctttttggc tttatttatt taaaataaaa agaagttttc cggaaatttt tatttaactg
     7501 tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt gcaaagaaag agggagtttc
     7561 attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca
     7621 tttttggtta ccagtccgta caatcttcgt tacttaacga actttacagg tacgacagga
     7681 ttagctgtta ttactttgga aaaagcattt tttattactg attttcgtta taccgagcag
     7741 gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg tcggacctat ttttgatgag
     7801 gtagcaaact tagtacgcaa agaagaactg cactcattag cttttgagga aacaacagtc
     7861 tcatttttag aatattcggt tttagaagaa atcatcgatg cggaattagt acctgtttca
     7921 cagatgatcg aagaactcag agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa
     7981 gcatgtcata ttgcggatat ggcatatgat cacatcctga aaatgatcca accgggcatg
     8041 actgagattg aagtcgcaaa tcaactagac ttttatatgc gttctcttgg cgcttcaggt
     8101 gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca
     8161 agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg actttggttg ctattacgaa
     8221 gggtacgttt ccgatatgac acgtacattc gctattggtg atcctgggga aaaattaaaa
     8281 gaaatttatc aaatagtctt agatgctcaa ttagcagtta ttgatgctgc aaaaccagga
     8341 atgactggtg tccaattaga tgcagttgct agagattata tttcaaaaca tggttacggg
     8401 gaagcatttg gacattctac aggacatggg atcggtttag agatccacga aggaccaaac
     8461 gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga tgaaccaggg
     8521 atctacttac caggaatcgg tggagtacgt attgaggatg acttgttgat cacagaagat
     8581 ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca tcttatagtt tcatcaatag
     8641 aagaagtaag cgtaaaaatc agtgttcaca gactttgtta tagaaaatca ctcgatttaa
     8701 tgatacacta tagaagaact tttaagctgt aagagataat aaagttgggt gttttctcca
     8761 attcatagca gtttgcagct gatcggcggc agttttctta ccacgtgatc actatgccgc
     8821 atcatagaga attcggatgt atctcgtgct taggcaaagg gaggaataaa taaatggcag
     8881 atgaaaaaaa tttgatttta aacgcaaacc aagatttagg agagatcgtg atcgctcctg
     8941 aagtcatcga agtaattatc gggattgcag cttctaaagt agaaggcgta tacggaatgc
     9001 gcggaacttt tgccagcaat gtcactgaat tattaggacg tgcagctcac ggaaaaggtg
     9061 tttacttagc aaacgaggaa gatggcttga aagtagatct ttactgttat ctagaatatg
     9121 gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag caagtccttt
     9181 tcatgacgga tatcgattta tctgaagtca atatccatgt agtagctgtt gttcctgaaa
     9241 aattagaaca accaacaatc gaagaacttt ttgaagacga agaggaagag aatgaataaa
     9301 gaattatcaa gacacgagat tcgggaaatg gcattgcagg ctttatttcc attagacttc
     9361 aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac
     9421 atgatcaacg aagatgaatc agagtttgtt cctgtttatc ttgatacgct agttggcggt
     9481 gtctgtgcaa aaaaagatga attggataaa gtgattgaaa aacatttaaa aaacaattgg
     9541 cacatcaatc ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg
     9601 acttatgtga cagatgtacc agcggctgtt gctttaaatg aagcaatcga gttggcaaaa
     9661 acatttagtg atgaccgttc acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa
     9721 gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat
     9781 aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg
     9841 gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa
     9901 atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa
     9961 aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc
    10021 cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt
    10081 gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag
    10141 catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt
    10201 catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca
    10261 tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta
    10321 gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat
    10381 gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca
    10441 gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca
    10501 ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat
    10561 gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta
    10621 gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga
    10681 gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa
    10741 agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat
    10801 ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata
    10861 tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg
    10921 aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat
    10981 gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa
    11041 gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca
    11101 aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg
    11161 gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac
    11221 caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat
    11281 acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag
    11341 cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa
    11401 acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct
    11461 gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct
    11521 agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct
    11581 cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta
    11641 gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa
    11701 caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca
    11761 gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg
    11821 aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg
    11881 aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc
    11941 accgagattc atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga
    12001 caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc
    12061 actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc
    12121 agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc
    12181 taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga
    12241 cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg
    12301 tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg
    12361 cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg
    12421 ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag
    12481 aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc
    12541 ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct atattggccg
    12601 gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc
    12661 ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg
    12721 ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta
    12781 ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag
    12841 cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag
    12901 cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa
    12961 cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa
    13021 agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag
    13081 aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg
    13141 tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg
    13201 attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga
    13261 aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt
    13321 caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa
    13381 agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg
    13441 atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta
    13501 caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa
    13561 ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga
    13621 tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga
    13681 gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa
    13741 aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt
    13801 tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga
    13861 aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc
    13921 agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat
    13981 tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata
    14041 agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc
    14101 ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg
    14161 ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa
    14221 aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag
    14281 ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg
    14341 cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag
    14401 cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg
    14461 ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc
    14521 tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt
    14581 tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga
    14641 gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg
    14701 ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact
    14761 accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga
    14821 caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg
    14881 tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg
    14941 acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt
    15001 tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg
    15061 tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat
    15121 agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga
    15181 agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt
    15241 agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt
    15301 ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga
    15361 aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat
    15421 tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat
    15481 ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga
    15541 aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga
    15601 ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg
    15661 taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat
    15721 ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga
    15781 tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt
    15841 ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt
    15901 ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg
    15961 aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt
    16021 gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa
    16081 agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga
    16141 agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc
    16201 aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt
    16261 ttcagcagct gatagctata ttttattttt acatgacact cattacagca gtcaatacaa
    16321 cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag
    16381 tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag
    16441 tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta
    16501 aataataggt agttacgata caaataaata atgttaatag aactagccat ataaaggcat
    16561 catccctttc agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg
    16621 gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg
    16681 atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag
    16741 tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat
    16801 tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg
    16861 cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt
    16921 cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat
    16981 aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg
    17041 aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat
    17101 tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca
    17161 cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg
    17221 gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata
    17281 tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt
    17341 gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca
    17401 gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt
    17461 ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa
    17521 ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca
    17581 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
    17641 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
    17701 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
    17761 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
    17821 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
    17881 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
    17941 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
    18001 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
    18061 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
    18121 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
    18181 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
    18241 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
    18301 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
    18361 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
    18421 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
    18481 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
    18541 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
    18601 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
    18661 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
    18721 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
    18781 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
    18841 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
    18901 tatgatctag gggtttcctc tcctcagcta gatgaagcag aacgtggatt tagttatcat
    18961 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
    19021 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
    19081 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
    19141 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
    19201 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
    19261 gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc
    19321 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
    19381 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
    19441 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
    19501 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
    19561 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
    19621 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
    19681 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
    19741 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
    19801 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
    19861 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
    19921 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
    19981 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    20041 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
    20101 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    20161 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    20221 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    20281 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    20341 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    20401 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    20461 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    20521 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    20581 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    20641 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    20701 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    20761 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    20821 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    20881 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    20941 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    21001 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    21061 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    21121 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    21181 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    21241 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    21301 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    21361 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    21421 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    21481 tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag
    21541 tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg
    21601 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    21661 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    21721 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    21781 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    21841 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    21901 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    21961 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    22021 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    22081 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    22141 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    22201 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    22261 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    22321 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    22381 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    22441 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    22501 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    22561 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    22621 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    22681 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    22741 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    22801 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    22861 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    22921 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    22981 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    23041 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    23101 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    23161 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    23221 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    23281 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    23341 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    23401 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    23461 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    23521 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    23581 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    23641 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    23701 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    23761 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    23821 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    23881 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    23941 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    24001 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    24061 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    24121 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    24181 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    24241 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    24301 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    24361 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    24421 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    24481 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    24541 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    24601 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    24661 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    24721 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    24781 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    24841 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    24901 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    24961 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    25021 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    25081 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    25141 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    25201 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    25261 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    25321 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    25381 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    25441 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    25501 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    25561 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    25621 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    25681 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    25741 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    25801 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    25861 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    25921 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    25981 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    26041 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    26101 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    26161 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    26221 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    26281 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    26341 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    26401 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    26461 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    26521 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    26581 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    26641 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    26701 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    26761 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    26821 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    26881 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    26941 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    27001 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    27061 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    27121 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    27181 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    27241 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    27301 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    27361 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    27421 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    27481 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    27541 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    27601 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    27661 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    27721 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    27781 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    27841 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    27901 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    27961 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    28021 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    28081 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    28141 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    28201 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    28261 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    28321 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    28381 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    28441 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    28501 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    28561 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    28621 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    28681 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    28741 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    28801 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    28861 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    28921 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    28981 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    29041 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    29101 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    29161 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    29221 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    29281 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    29341 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    29401 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    29461 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    29521 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    29581 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    29641 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    29701 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    29761 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    29821 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    29881 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    29941 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    30001 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    30061 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    30121 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    30181 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    30241 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    30301 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    30361 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    30421 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    30481 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    30541 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    30601 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    30661 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    30721 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    30781 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    30841 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    30901 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    30961 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    31021 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    31081 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    31141 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    31201 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    31261 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    31321 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    31381 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    31441 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    31501 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    31561 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    31621 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    31681 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    31741 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    31801 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    31861 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    31921 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    31981 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    32041 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    32101 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    32161 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    32221 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    32281 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    32341 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    32401 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    32461 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    32521 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    32581 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    32641 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    32701 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    32761 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    32821 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    32881 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    32941 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    33001 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    33061 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    33121 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    33181 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    33241 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    33301 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    33361 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    33421 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    33481 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    33541 ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg
    33601 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    33661 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    33721 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    33781 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    33841 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    33901 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    33961 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    34021 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    34081 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    34141 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    34201 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    34261 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    34321 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    34381 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    34441 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    34501 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    34561 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    34621 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    34681 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    34741 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    34801 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    34861 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    34921 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    34981 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    35041 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    35101 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    35161 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    35221 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    35281 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    35341 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    35401 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    35461 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    35521 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    35581 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    35641 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    35701 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    35761 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    35821 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    35881 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    35941 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    36001 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    36061 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    36121 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    36181 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    36241 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    36301 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    36361 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    36421 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    36481 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    36541 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    36601 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    36661 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    36721 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    36781 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    36841 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    36901 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    36961 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    37021 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    37081 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    37141 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    37201 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    37261 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    37321 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    37381 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    37441 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    37501 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    37561 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    37621 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    37681 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    37741 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    37801 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    37861 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    37921 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    37981 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    38041 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    38101 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    38161 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    38221 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    38281 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    38341 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    38401 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    38461 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    38521 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    38581 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    38641 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    38701 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    38761 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    38821 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    38881 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    38941 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    39001 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    39061 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    39121 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    39181 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    39241 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    39301 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    39361 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    39421 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    39481 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    39541 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    39601 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    39661 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    39721 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    39781 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    39841 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    39901 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    39961 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    40021 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    40081 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    40141 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    40201 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    40261 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    40321 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    40381 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    40441 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    40501 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    40561 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    40621 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    40681 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    40741 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    40801 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    40861 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    40921 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    40981 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    41041 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    41101 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    41161 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    41221 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    41281 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    41341 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    41401 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    41461 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    41521 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    41581 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    41641 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    41701 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    41761 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    41821 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    41881 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    41941 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    42001 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    42061 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    42121 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    42181 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    42241 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    42301 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    42361 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    42421 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    42481 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    42541 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    42601 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    42661 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    42721 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    42781 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    42841 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    42901 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    42961 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    43021 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    43081 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    43141 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    43201 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    43261 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    43321 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    43381 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    43441 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    43501 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    43561 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    43621 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    43681 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    43741 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    43801 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    43861 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    43921 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    43981 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    44041 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    44101 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    44161 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    44221 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    44281 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    44341 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    44401 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    44461 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    44521 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    44581 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    44641 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    44701 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    44761 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    44821 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    44881 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    44941 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    45001 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    45061 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    45121 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    45181 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    45241 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    45301 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    45361 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    45421 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    45481 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    45541 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcatactgcc
    45601 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    45661 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    45721 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    45781 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    45841 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    45901 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    45961 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    46021 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    46081 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    46141 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    46201 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    46261 atatattgca gaaccattcc tagggttatc tcaagggttg ggagcagtat tgatcgtcac
    46321 agtatttgtt caagtattct ggttctttgg tattcatgga tcaaatgtat tggcaccagt
    46381 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    46441 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    46501 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    46561 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    46621 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    46681 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    46741 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    46801 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    46861 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    46921 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    46981 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    47041 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    47101 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    47161 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    47221 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    47281 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    47341 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    47401 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    47461 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    47521 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    47581 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    47641 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    47701 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    47761 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    47821 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    47881 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    47941 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    48001 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    48061 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    48121 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    48181 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    48241 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    48301 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    48361 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    48421 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    48481 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    48541 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    48601 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    48661 gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    48721 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    48781 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    48841 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    48901 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    48961 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    49021 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    49081 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    49141 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    49201 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    49261 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    49321 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    49381 ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt cattctacct
    49441 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    49501 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    49561 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    49621 cagtcaggaa cgtataaata acccctgtat cctttgcggt acaggggttt gttttgtatt
    49681 tgtgccaaaa atgtgccaaa ctagttatca tttttatata aactatcgaa cacagtattg
    49741 attttttctt tatcctgatc ttcaagctct cgaacaatat gaagataagt agacattgta
    49801 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
    49861 agtaaaatag atgcatgtgt atgccttagt ccatgcaaag taaattgctt ttcaatccct
    49921 aattctttta attttttgcg caaatatttg ctgacggcgt ttgatgaaac taatccattt
    49981 tttaagttga aaaaaacaaa attatgaggg tttttgattt caaaattctc gaataattct
    50041 ttttgattga ttttaaactt ttttaagaga tcaatcgtat gtttatcaat ggaaattttc
    50101 ctatttgaag tttcgttttt tgtttttccc cattcattta atttataatt ccatgtttta
    50161 ttaatagtga tgatttgatc ttcgaaatcg atatcttccc atgttaatcc tagaagttca
    50221 gcaaatcgca ttccagtggc accagcaact aacacgagca taggagaaga gtactgcgca
    50281 gaaaggtttt cttctacgag tttcattaaa gatttaaatt gatcataatc taaatattta
    50341 tcttcttttg gcttcaatga atcttttcct ttgattactg cttttctagt tggatcaaac
    50401 ggaattaatc cttcttctac ggcatctttc aacgacgctc taatatggtt attaaactta
    50461 ataacggtag attttacatg gtctttagca tacttattta aaaagcgttg atatcccact
    50521 ctatctaaat cagaaatcaa taccgctggc atatatttct ttatgttcat taacgtatct
    50581 tcgtattttc tataagtgat aggagatact gttccttctt tataaagttg catccaatcc
    50641 tcaaagtaat ctgataataa tagatttttg cgatcccctt taaggccttt gttcaagtta
    50701 tattctaatt cgttggcggc atcttttgct tctccttttg tcttaaatcc tgattttctt
    50761 atcttcgtgt attttccgtc atcttttttg tatgaaattt cgtattgcca actatttcca
    50821 cgtttgacta atcttgccat aattgaatca gctctctttc tctgatacaa taggaactat
    50881 aaagaagccc attgtttggg ttttattatt ttttgcacat cctttttgct tgccggcgca
    50941 aggatgtgct atttgtcgtt ttaatggtat aattggatgt gtattagatc atccaatcag
    51001 aggtgtttat tatgattaaa gtgattttta aaagaataac tgaacttcca gagtttagat
    51061 tatttgttga ttattggtat gtctggtttt ttattgttgc attgctgata ggtctagatg
    51121 tcatattaga aagaaaactt aagtaagagt tgagttaata aattgaattc ctaaatgaga
    51181 tattttgtag cccatatttt gtttgccgac atcacctaat actatggagc taatcgtttt
    51241 ttctaacaga ccaaaatcag ataatctatt taagctacca tctattagct cttgtgaata
    51301 gccagacatg ctgatatttc cagtgaaatt ttcgtttcca cgactcatta agcatgctaa
    51361 caattgaatc tcaatcgatg ttaattgatt cagtacaaga agataatact cctcgttatc
    51421 ccatgaagcg ttgttaattc gaagtaaaca attcttataa agatttttat acatatctat
    51481 tttattttgt gcctttgatt tttctacctc gtcatttatc ccttcgaata ttccaatgag
    51541 ttggtccttg ttctctaagt tgaaaacatc ttctggaatt tgatcgcgaa tgttttctaa
    51601 tgattcgttt aaatttttgt agaatttctc gatgcgtttg aaacgcttct cgtttttaga
    51661 cccaaaataa agtgactgca caacgccacc aatataagga attgctccaa taccgccttc
    51721 aactattaat tcaagatatt cttttttggt tagtttttca ttgctattat ccatagttac
    51781 ctcctatttt gtcatttcct ttacaagcat tgtgactcct gatggcactc ttttgtatag
    51841 gttttattat tgtgcacaat ctaactctta gtggggcgtg gttcatttat tttttactta
    51901 ctttactatt aatcatatcc cattgagcat aggatttttc tatgttcgat cctcttgaac
    51961 taatttctcc tgtaaaagtt actttatcgc cctttgtaaa ggtatcagca ttgtttaagt
    52021 ccttcgcaaa aattacatat gaaactttag gtgttgcaga aatatcattc caacttttat
    52081 catcgtactc tttgtctgct ataatggcta ttctagtagt cattgattcg ataacagttc
    52141 cttcaaaagt atatgtttta ccactaatat atttagtaaa agctgtattt tgctcagtag
    52201 ctggagtaat agaataataa ctatctaaga aagcacccca gtcatgatta tttgtctcta
    52261 aaaattcttc aaattgagtt aggcttttat tgctgctgtt agatgtagta gtttgagtat
    52321 cagatgatga gctttcctgt ttgttattgt catttgcctc gtttgatgag gaaagcgaga
    52381 aacctacggt agtaaaaata atcccgataa taaggagaat cataggtgtt ttcttatttg
    52441 tttttttgat aaatcttaca attaacatga ttaaacctat aataaataaa acaataccag
    52501 taaaaccaaa tataccgtcc attaatagat cctctttctc ttttgatatt gaatattata
    52561 aaccaagcaa ttcttttttc ttaaaatcga attcttcttg attaataatc ccttcatcta
    52621 acaactcttt atacttttta atttcatctg cacttgaaat ttgtgtttct ttggaattat
    52681 tatctaatat agattgttgc ttctctatat atttttttaa attttccatt tcattccaat
    52741 atttttttgt aaacataacg gtgttttcat ctttcgtggc cgctaatata cctccgcgac
    52801 tttcgtttcc gcctagcaag gtaaattgta tataaccatt actcatacct ggttttttta
    52861 attgtacggc tgatatattt ttaaagggga tagttttttc tcctttaata ccttgattaa
    52921 ctaaatttat aaatcctttt ctaataattg aaatagaatc atcagttact ttaatcaagg
    52981 tttttcctgg agattttatt aaaatctctt tttccatttt ttcttcctca tttctgtgat
    53041 atgatttttt ttgtagaagc tcatatatga ggaaagagcc cgtgtt
//
LOCUS       DAZBWR010000006        51536 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000006 DAZBWR010000000
VERSION     DAZBWR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.006"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..268
                     /locus_tag="ACHGT5_000336"
     CDS             95..268
                     /locus_tag="ACHGT5_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216355.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(334..942)
                     /locus_tag="ACHGT5_000337"
     CDS             complement(334..942)
                     /locus_tag="ACHGT5_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN7216356.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACHGT5_000338"
     CDS             complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACHGT5_000338"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN7216357.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(1969..2940)
                     /locus_tag="ACHGT5_000339"
     CDS             complement(1969..2940)
                     /locus_tag="ACHGT5_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN7216358.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACHGT5_000340"
     CDS             complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACHGT5_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFN7216359.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACHGT5_000341"
     CDS             complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACHGT5_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFN7216360.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACHGT5_000342"
     CDS             complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACHGT5_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN7216361.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACHGT5_000343"
     CDS             complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACHGT5_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN7216362.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(7382..7549)
                     /locus_tag="ACHGT5_000344"
     CDS             complement(7382..7549)
                     /locus_tag="ACHGT5_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216363.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(7707..7892)
                     /locus_tag="ACHGT5_000345"
     CDS             complement(7707..7892)
                     /locus_tag="ACHGT5_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN7216364.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            8037..8408
                     /locus_tag="ACHGT5_000346"
     CDS             8037..8408
                     /locus_tag="ACHGT5_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7216365.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            8666..8995
                     /gene="mobC"
                     /locus_tag="ACHGT5_000347"
     CDS             8666..8995
                     /gene="mobC"
                     /locus_tag="ACHGT5_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFN7216366.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            8956..10284
                     /locus_tag="ACHGT5_000348"
     CDS             8956..10284
                     /locus_tag="ACHGT5_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN7216367.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            10281..10652
                     /locus_tag="ACHGT5_000349"
     CDS             10281..10652
                     /locus_tag="ACHGT5_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN7216368.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            10835..11071
                     /locus_tag="ACHGT5_000350"
     CDS             10835..11071
                     /locus_tag="ACHGT5_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216369.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(11371..11574)
                     /locus_tag="ACHGT5_000351"
     CDS             complement(11371..11574)
                     /locus_tag="ACHGT5_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFN7216370.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(11578..12522)
                     /locus_tag="ACHGT5_000352"
     CDS             complement(11578..12522)
                     /locus_tag="ACHGT5_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216371.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(12523..16443)
                     /locus_tag="ACHGT5_000353"
     CDS             complement(12523..16443)
                     /locus_tag="ACHGT5_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFN7216372.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(16592..18673)
                     /locus_tag="ACHGT5_000354"
     CDS             complement(16592..18673)
                     /locus_tag="ACHGT5_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN7216373.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(18670..19899)
                     /locus_tag="ACHGT5_000355"
     CDS             complement(18670..19899)
                     /locus_tag="ACHGT5_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFN7216374.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(19889..20140)
                     /locus_tag="ACHGT5_000356"
     CDS             complement(19889..20140)
                     /locus_tag="ACHGT5_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFN7216375.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(20163..22154)
                     /locus_tag="ACHGT5_000357"
     CDS             complement(20163..22154)
                     /locus_tag="ACHGT5_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFN7216376.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(22186..24585)
                     /locus_tag="ACHGT5_000358"
     CDS             complement(22186..24585)
                     /locus_tag="ACHGT5_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFN7216377.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(24515..24937)
                     /locus_tag="ACHGT5_000359"
     CDS             complement(24515..24937)
                     /locus_tag="ACHGT5_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFN7216378.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(24951..25487)
                     /locus_tag="ACHGT5_000360"
     CDS             complement(24951..25487)
                     /locus_tag="ACHGT5_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFN7216379.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(25524..26387)
                     /locus_tag="ACHGT5_000361"
     CDS             complement(25524..26387)
                     /locus_tag="ACHGT5_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFN7216380.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(26479..26694)
                     /locus_tag="ACHGT5_000362"
     CDS             complement(26479..26694)
                     /locus_tag="ACHGT5_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFN7216381.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(26771..28579)
                     /locus_tag="ACHGT5_000363"
     CDS             complement(26771..28579)
                     /locus_tag="ACHGT5_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFN7216382.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(28576..29052)
                     /locus_tag="ACHGT5_000364"
     CDS             complement(28576..29052)
                     /locus_tag="ACHGT5_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFN7216383.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(29127..29339)
                     /locus_tag="ACHGT5_000365"
     CDS             complement(29127..29339)
                     /locus_tag="ACHGT5_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN7216384.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(29361..30326)
                     /locus_tag="ACHGT5_000366"
     CDS             complement(29361..30326)
                     /locus_tag="ACHGT5_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFN7216385.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(30817..31338)
                     /locus_tag="ACHGT5_000367"
                     /pseudo
     CDS             complement(30817..31338)
                     /locus_tag="ACHGT5_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(31354..32052)
                     /locus_tag="ACHGT5_000368"
     CDS             complement(31354..32052)
                     /locus_tag="ACHGT5_000368"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN7216386.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(32009..33670)
                     /locus_tag="ACHGT5_000369"
     CDS             complement(32009..33670)
                     /locus_tag="ACHGT5_000369"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN7216387.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            33888..35288
                     /locus_tag="ACHGT5_000370"
     CDS             33888..35288
                     /locus_tag="ACHGT5_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN7216388.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(35540..36619)
                     /locus_tag="ACHGT5_000371"
     CDS             complement(35540..36619)
                     /locus_tag="ACHGT5_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7216389.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(36705..37664)
                     /locus_tag="ACHGT5_000372"
     CDS             complement(36705..37664)
                     /locus_tag="ACHGT5_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN7216390.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(37676..39187)
                     /locus_tag="ACHGT5_000373"
     CDS             complement(37676..39187)
                     /locus_tag="ACHGT5_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN7216391.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(39209..39772)
                     /locus_tag="ACHGT5_000374"
     CDS             complement(39209..39772)
                     /locus_tag="ACHGT5_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN7216392.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(39778..40641)
                     /locus_tag="ACHGT5_000375"
     CDS             complement(39778..40641)
                     /locus_tag="ACHGT5_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7216393.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(40655..41551)
                     /locus_tag="ACHGT5_000376"
     CDS             complement(40655..41551)
                     /locus_tag="ACHGT5_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7216394.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(41726..43015)
                     /locus_tag="ACHGT5_000377"
     CDS             complement(41726..43015)
                     /locus_tag="ACHGT5_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7216395.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(42987..44504)
                     /locus_tag="ACHGT5_000378"
     CDS             complement(42987..44504)
                     /locus_tag="ACHGT5_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN7216396.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            44702..45592
                     /locus_tag="ACHGT5_000379"
     CDS             44702..45592
                     /locus_tag="ACHGT5_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN7216397.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACHGT5_000380"
     CDS             complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACHGT5_000380"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN7216398.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(46501..47973)
                     /locus_tag="ACHGT5_000381"
     CDS             complement(46501..47973)
                     /locus_tag="ACHGT5_000381"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN7216399.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(48610..48972)
                     /locus_tag="ACHGT5_000382"
     CDS             complement(48610..48972)
                     /locus_tag="ACHGT5_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN7216400.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            49195..49947
                     /locus_tag="ACHGT5_000383"
     CDS             49195..49947
                     /locus_tag="ACHGT5_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN7216401.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(50087..51010)
                     /locus_tag="ACHGT5_000384"
     CDS             complement(50087..51010)
                     /locus_tag="ACHGT5_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7216402.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(51025..51297)
                     /locus_tag="ACHGT5_000385"
     CDS             complement(51025..51297)
                     /locus_tag="ACHGT5_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216403.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        12835 a        12717 c        11439 g        14545 t
ORIGIN      
        1 gaggggcaag gctttttttg agtggtggaa gtggattaga gcatggcaat agcaaaacag
       61 catagcggca tcctcctatg ggccgccaca ccttttgctg ccagaaaaga acggcggcct
      121 tgatttctca aaaccgccgc cagcaaaata caaaatgggc gtgggaaagg tctgcccaac
      181 agcggttttt ttcttttctg ccgctgggca gattgaatac tataatttgc aatgtattac
      241 aaaagatgct ttaaccactt tccagtaaaa gattgtccat gaccagcata gattttatca
      301 aatccaaaat ctgatacaaa accaatactt tcattatgaa acggcaaaat taaaataggt
      361 atcggggtat ggctcatcaa tcaacttata gtgccaccat tcaaagcgat aagactgaaa
      421 tccgctgctt tccatgattt tacgcaagca tcttcgattt tgtgcctctg catcccctat
      481 cccttttgct gtatgatgcg accgttcgtc cataaaatcg aagttacttc ccattgaaac
      541 aagttcccct gtatccaggt ggtagagcgt aagatcaatt gcgcttccac ggctatggct
      601 ggattgtgag gccacataac cctttgtaat caactcggct cgctctatat tggggtaaaa
      661 tttttctttt gtgagattgt cctccggttg cgctgcccaa cgcaggaagc agtccaccgc
      721 agttcttggc cgatatccat cccataaaag caagccatag ccaagcgcag ccgcatggat
      781 ttgtgcctcg cgcagagcaa acgccacggc ctttgtgccg atgatgcgat tcacctcata
      841 cccatccacc ggttttcctg tgaagttatc ccatgtggcg tacttggcat cccaacggac
      901 gccatgcaac atttcatcta aaaacaaaaa accattttcc atacttatca cctctttaac
      961 gccaatgtaa tcaggctgtc aatcagtgca ggaagcgtga ttcctgcggc ggccatcata
     1021 cgtgggtagc ggctgtacga tgtaaaaccg ggcagggtat tgacctcatt tagaacgatg
     1081 ccgccatcct cctgcaaaaa aagatcaaca cgggcaagcc ctctgcatcc aagcacccga
     1141 tatactttct ttgccgtttc ctgcacccga tttcgttcct cgaccggaat gtctgcggga
     1201 actgtaatca tcgcattttc tgagcctttt tccggctcgt tttcctgatg gatgcggaag
     1261 gtaccgtggc tcagccggat ttgatccact tcgccgacaa tcaaatcatc ctcgttcccc
     1321 atgaccgcac acccgacctc acagcccgaa atcgcttgct caattaagat ttttccatca
     1381 tattgtcctg ccgcttctat cgcagcgtta agttcttccg taccgtttac tttggttacg
     1441 ccaaaggacg aacctgaccg tgccggcttc acaaagacag ggtaggtaag cgcacccgcc
     1501 tccggcttgt cacctttatc aatcatttga aattcgggaa cggcgatgcc cgcatttttt
     1561 gtaagaatgt aggccagtga tttgtccatg caagctgcgg agctttgaat atcacagccc
     1621 acatagggga taccagacaa tacaaacagc ccctgtatcg caccatcctc cccgcatttg
     1681 ccatgcaaaa ccgggaaagc cacatcaata cgccgtgttt cgtattcgct ttctttcatg
     1741 acaagcagcc catgcgtttt cctatccggg gagagtatgg cggggagact gtcggcttcc
     1801 cattccgtac atggcttctt gcatagcttc catacaccgt tttttgtaat tccgatgtag
     1861 tgcggatcga atttttccgt atcaatgttc gcagcaattt ctattgcgga ttttaccgac
     1921 acatcatgtt cctccgagca accgccgaag atgattgcga cttttattct attcatgctg
     1981 taaactcctt tcaaaattga gacaattcct gattgttttt tctgtagtat cctgcagcac
     2041 acgctcagtg tagtacgccg tatggggtgt gatgatcaca ttcggcatcc ttaaaagctg
     2101 cgacaaaaag ggatggtcaa gcactttctg cgagcaatcg gtataaacaa actgatcctc
     2161 gccctccaac acatccagtg ccgcaccacc cagctttccg ctttccaacg cctccaccag
     2221 cgccccggta tcgacaagcg ctccgcgccc ggtgttgatt aaaaatgcgc cttgcttcat
     2281 ctctccaatt tgcttctgac cgatcagatg gcgggtatcc gcacaaagcg gcacatggag
     2341 cgtaacaata tcgctgtttt ttagaagctc atcaagctgg acataatctg catccatttt
     2401 tcggctgtta tcataggcca gcacatggca tccaaatccc cgcagccttt tgacgaccgc
     2461 ttgcccgata tggccggttc caataactcc cacagtcata tcccccagtt ctttcccccg
     2521 gatacgatcc aatctgaaat cttgttgcgc cacggcgtgc atggtgggtt ttgcaccccg
     2581 tatggccatc agcatcagca tcaaagcata atccgcaacg ctgtccggcg aatacgcaac
     2641 cgtgccaacc gagattccca ttctctcggc ggcagtcgta tcaatgtgat cgcagccgat
     2701 gctgcgggta gaaatgtatt ttaccccgac ctttctcagc gcaagaattg tcgcctcgga
     2761 aacctcggac ttatggccta cgctaacgca ttgattgcca gcggccaatt ttgcgttgtc
     2821 tgccgatatc gcatcgctaa tcagcgtagg gataatatga aaatccggcg ataaagcgcg
     2881 gaaagcattt gcctcatcct gttcgcatcc aaaaacagta atgcccacac tttttctcat
     2941 tggttctcct cctgaatatc cacactttcc gggaggggat aacagatttt gcatttgttt
     3001 gtataaagca gcttgcaatc tattatttcc ccggtaccta aggcaacttg catccgttta
     3061 acctgggcat attcataaat cccgccactt tcacggacat actggatact gccatttgta
     3121 attttgtata atgcttgggg ctctttgtcg gcggacacct gaccgatgat tttctcatca
     3181 tctaaggtca cccatatttg gtaggtgcag tcggtatcgt ttcgcacttt taaatcaatc
     3241 cagccctctg aaatggttgc atccacccct ttaatctcgt cactgtttgg ctctggaaac
     3301 tcctttactt cgtgacctct gcgctggata attgtcaatg gcgtatgcag gaacatccaa
     3361 aatagtaaat tgctcatctg gcacataccg ccgcccgaca tggtggtgag tttaccattg
     3421 gtcaccgtaa ggccgtcttt atagggggta tctttgtccg catggcgtac cagccaccag
     3481 aaagaaaagg gttcccccgg tcttatcaat aagccgttta aggtctttgc tgccagcttt
     3541 aagttgaaca ccttgttttc ctggtatatc atgtcaaatc cggtattgtg gttgtataat
     3601 gcgcaatccg tttcaaagag caaatgggaa agcgattttt cccctatcgt ctgcgcatag
     3661 cgacagccgt caaatctcat tcccgcataa aagcacattt ttctctgcgc ccgtctcatt
     3721 ggaagcagga acgggaaacg ctgtgtcaat ctttttctgt ccataacata acatcctttt
     3781 ctcatttttc tggttttaaa tattcctcaa ggcaaacccc ttggttgtac atctctgtgg
     3841 cagcttcgat gccaacatat cggtaatgcc acggctcgtt gctcacaccg gttatctctg
     3901 tcttgtctgg tgggtagcga cgaataaaac caaaacgata gctgttttca tccagccatc
     3961 tataaacctc gttgcctgtt gaatgaattc cgtccgcatt gatatccaca gcaagaccaa
     4021 gctgatgctc gctcgttccc ggcacggcca cccaagtttc tgcttccgct ttagcctgtg
     4081 cagaggtgta gcctttcgcc ttatattcgg caattttttc atccataatt tcttgctgtt
     4141 tttctgttgt tcggtatccg gatgcgacaa tcgggtaaac tccatcagtt cttgcggcat
     4201 caaacaaatc ttgaagatag ggagaaatcc gaatatctat ccgctcacca tttgatagtt
     4261 gctcaagctc cacatcgtac tgtgcgggga tggggttctg cctgttcact aaaatcaggc
     4321 tccattcctg ttcatctccc tgttctttgg cggtggcaag agcgttttcc tccaaattct
     4381 ccactacgtc agtgctgctg cctgtatgag ttgcagggat gggcggtatc tgtgttgcct
     4441 ccaatacgga agccagtctc cgccccaaaa acagcgtgca gactgtgaac gaaataatga
     4501 acgcacatag aaaagcccgc ttttccacgg attgtttcat atgccgtttg tgatgatccg
     4561 cattggaatg atagttgctt ttttccattt gatcgaatgc ctcctttgca ataaaactac
     4621 gatttgtggc tggtacgatt gttattatgg caagcgcatc tgtactttcc ctgtagccag
     4681 tctgtagttt tcctgtagca gcaaaaaaag accggcaggg ttatacctgt cggtcaaaat
     4741 catttaagtt ttaaatattt acaatgttga tgtgagcggt aaatccagcc agaacaaaac
     4801 gccatccgag gtgttttcca gcgcatactg gaggctcatg gcatccagcg ttttttgtac
     4861 gatggcaagt cctaaaccgc ttcgcccact ttttctgctt tgcgcctgat caatgcgata
     4921 gaatggggtg aacagccttg gaagcgcagt atcatcaatg tgaacgccca tgttcaaaac
     4981 aaaaaggcgg catttttcag caccaggctc actccatatc cgtacctcgc ctccctgcgg
     5041 cgtgttctga actgcattca atatgacatt ggatagtgcc ttttggagca gcctcggatc
     5101 ggacaggaca atttgcccgg ctggaatatc tgtgacgaac cgctggttgt ttgcctctgc
     5161 caaggtttga aaatcgggca gcaactcggc aaccgtgcgc cctatgtcca acggttcagc
     5221 tatgggtacg attctcccat cattcaggct gacaagctcc agtatttcgg aaatgatttt
     5281 gccctgccta tccatcattt tgatgcattc gcgcagatac ttagaatgat ctttgtagtc
     5341 accgatattt tcaagcattc cctccaacag aacgcttgta gccgcgatgg gcgtttttag
     5401 ctcatgagaa gctgccgcaa agaaatatcg ctgtgtttcc tccaactcat gttcccttgc
     5461 gatttcatcc tccagccttg cgatggtttc tttcagcctg acatacatgg aatgcatatc
     5521 atgagccagt gcgccaagct catcctttcg ctccagcggc ggcggtacat ctttcaggtt
     5581 tgccatttta ttcgcactgt ccgctaaggc tttgatcggc gttgtcattt gccgcgcaaa
     5641 gatatacgcg cataaaaggc tgaatacaac cattatcgca atcataacaa ttccccgaat
     5701 tgtcagccct tgataaagca atcccacacc tgccttgctt tgagcaacaa tcgaaatatt
     5761 atcatctcta tgtaccacat aaagaaagtc gggtctaaaa ctatttgatg tattggcatt
     5821 tggtgtggca tagagtacgc ttccctcttt atcttcaata taaaactcaa aggattggtt
     5881 attgtagtga aacagccctg ccacctcttg aatatcaagc ctatcgctat tctgaatcag
     5941 ttccaccaat ggctgatagg attttactgt ttgctgtaat tccatcactc tgaaataaga
     6001 cacaaattgc tgtgcaaaca gtgttgctgt tacaccgaca agcagtagca gggcaatgat
     6061 cgtataggaa aaaaccttaa tgaaaatccc ttttctttcc attattatga ttcctccaat
     6121 cggtatccta caccgcgaat ggttttgatg atattttccg gcagcttggc ccgcaaattt
     6181 ttgatatgag tgtggactgt gctgccatca ccctcaaagt catatcccca tatacgtgac
     6241 aaaatgattt catgcgttaa ggttctgcct ttgttctgca ccagcagcaa aaggatttca
     6301 aattcttttc gtgtcagggg cagctctgta ccgtcacaaa gtaccgtgaa atcctccggc
     6361 agaagtgtca gcctgccgac acggatttcc tttgccagcg caccgctgcg ccgcagcagg
     6421 gcttccaccc gctttagtaa aatctgcatc ttgaatggct ttgttacata gtcgtctgcc
     6481 tctgcatcaa acgcccggat ttggttttcg tcatccgaca gggctgtcat catcagaatg
     6541 ggagtatcat ttttcgcacg aaattcacgc aaaagttcat gcccgttcat accgggcagc
     6601 ataatatcaa gaataaccag ttgataagtg ttttcgtaaa acttggtgta tgcctcattt
     6661 ccatctgtgc aggcatccac ctgatatcct gcctcagcca gaaacgccct tactgtatta
     6721 cagatatgat catcatcctc gacaagtaga attcgtatcg acataaaaat ccctccattt
     6781 taagaagata acataacagt ctgtagtttc cctgtagttt caaagcgatt tcttttcgga
     6841 ttttaatcat ccgtcattgg gatactcgct tcttttcatg gtgtatgtat ctatattatt
     6901 ggtttaaacg aattaaatca agataataat aattaacacc ttcttaatgt ttgggatgtt
     6961 tcgattacag cggttgtcaa aacatcccga taatactggc aagcaatgga tcagaatatc
     7021 ctttacggat attctgatcc accacttgcc cgcctgcggc gttctcgttt tgcttgttgg
     7081 ggacacccca agcctcagca aaatgcgcgg cctctttttt gcgaatgcac cttgcggtat
     7141 ggcttttcac acaggcaggg cctgtgtgta ctccctccgg tcgctcttgc cgcactccgc
     7201 gcagcagatt cagtcctaat accgttcaag acagtagaaa atcagcaagt ctatattcct
     7261 catatcttca tcatccgtgt ccttgcagtt acaggaaatg taataatcgc caaatgtaaa
     7321 tcccagaata ttttcctcaa aaagccgtac ctccccggta ttaaaatcaa tcaccccata
     7381 ctcaaaggcg cattagacca gcagttatcc aatgtctgga gacttcacca taaagaatcc
     7441 agatgccggc cttttcaatt ctaccatata cggctgatac agcagcagct tcctcgtctt
     7501 tgtttcgggc attttccatt tctgtcatga taaagtttcg cttcttcata catccatgtg
     7561 taaatctttt ctttctgttt cagatgagaa tactttttat cggtctggag cagcctgcca
     7621 tctatcatct tatagttttt ccgtgacaaa ataccatgct ttctctctct tcaaaaccat
     7681 atccaaaatg cgccggtttc attatactat tcagtaaccg cctttccttt ctttgtacgc
     7741 ctgtcaatcg gcttttccgc agttttggga atgagccaga tcgtcgccat ctttacagcg
     7801 ccggggatgc gtcccgcagc acaatagtag tttatccatc gtgggggcac accccatttc
     7861 tcgcctgcct ctttcaacgt catgtagtcc atagtacacc tccacagggt acattataat
     7921 tcttttgctc gaagaataaa agaaacaaaa tgtgaaatat aaactttaag aaaggtgcat
     7981 ataccaacat tccacgggca gagtatgaaa tatacaatgt aagtgggagg tgcgatatgg
     8041 cgaatattga agattgtccc ggctttgaaa ctttcggcgc ggatgtgaaa gcggcccggc
     8101 aagcaaagcg cgtttcgcgc aaagcaatgg cagagaaaat aaatattgac tggcgctatc
     8161 ttgccaacat cgaaaacgac ggggccattc ccagtctgcc ggttgtcata cagcttatta
     8221 aagcctgcgg gcttcctgtg gaacgctact ttaacccgga gatcatgcgg gaggaaagcg
     8281 aactgcggca aagggtcagc cacaagctga aactctgccc cgaagaatac ctgccgatca
     8341 tcgagggcgc gattgacagc gccatactaa ttgaaaagcc gaagaagaat gaaacggagg
     8401 atgtgtaact ggcatcctct gttttacttt tagcgggagc tgcacagcgt agcttctatt
     8461 tttttgcact tttttctttc cagagggcta ttttgcgggt agtttggggc tttggaaacg
     8521 tcttgttagt ggagaaacag taacgtagca agcataggga gtgtagccac tcccggaaaa
     8581 acggcctgcc ccgtccctgc cgggccgcgt ccgttttgct tgttggggaa atccccaaac
     8641 ccctgaatgg aaaggacggt gaagcatggc aaacagaaag cgaaaaatcg tgctgcgcgt
     8701 ccctgtgacg ccggaggaac gggctatgat tgaacagaaa atggcgctgc tgcatacgaa
     8761 gaacttttca gcctacgccc ggaagatgct cattgacggc tatatcgtca acatggacac
     8821 cagcgacatc cgggcgcaga ccgccgagat acagaagatc ggcgtcaacg tcaatcagat
     8881 tgcaaagagg ctgaacggta tggggccggt gtacgcacag gacatcgagg atataaaggg
     8941 ggcgctggcg cagatatggc agttacaaag atacatccta tcaagtcaac gctgaaaaaa
     9001 gcccttgact acatcgagaa cccggacaaa actgacggca agctgctggt ggcctccttt
     9061 gcctgctctt atgaaacagc ggacattgaa tttgaaatgc tgctggcgca ggcataccaa
     9121 aagggcaata atctgggcca ccacctgata caatcctttg cccccggcga agccacgccg
     9181 gaacaggccc atgagatcgg gaagcagctt gccgaggaag tgctgcaagg gaaatatccc
     9241 tatgtgctga ctacccatat tgataagggc cacgtccaca atcacattat tttctgtgcc
     9301 gtggatatgg taaatcagcg caagtacatt tccaacaaac agagctatgc ctatatccgg
     9361 cgcaccagcg acaggctgtg caaggaaaac ggattgtccg tggtgaagcc cggccagagt
     9421 aagggcaaga gctatgccga gtgggacgcc cagcggaaag ggacgagctg gaaagcgaag
     9481 ctcaaagcag ccattgacgc cgctatcccg caggcaaaag attttgatga tttcctgcgg
     9541 ctcatgcagg cgcaaggcta tgagatcaag ccgggaaagt ttatttcttt ccgcgctccc
     9601 ggacaggaac gctttacccg ctgcaagaca ttgggggaag cctacaccga ggaagccatc
     9661 aaagagcgta tcaaaggccg cgttatcacc agagcgccca aagagcgcaa ggggatttct
     9721 ttgcggattg atctggaaaa caacattaag gcccagcagt cggcaggcta tgagaggtgg
     9781 gcgaagctcc acaatttgaa gcaggccgca aagactttga atttcctgac tgaacatggg
     9841 atagacacct acccggattt ggaaagcaaa gtggccgaga tcaccgccgc cagcgacgag
     9901 gccgccgcta ccctcaaagc tatggaacac cggcttgcgg atatggcggt gctgataaag
     9961 aatatttcta cctacaagca gctctggccc gtggctatgg aataccgcaa tgcagcggat
    10021 aaggcaaaat tccggcgtga gcatgaaagc acgttgattt tgtatgaagc tgcggcaaag
    10081 gccctaaagg gacagggtgt gaaaaagctc cccgatctgt acgccctgaa agccgagtac
    10141 aagcgattgg ccgaggaaaa ggaacggctg tatgagaagt acggcgaagc gaaaaagcag
    10201 atgcaggaat acggcatcat caagcagaat gtggacggca ttttgcgtat gacgccggga
    10261 aaggaatgga cgcaagagct atgagtattc cacgaatgaa ttaccctcaa tatcgcaagg
    10321 cccggaagct gacgcatgag tgctgcaact attgtgacgg caactgcctg ttgctggacg
    10381 acggcgaaga atgtatttgc gtacagagca tttcctattc gcttctgtgc cgctggttta
    10441 aggccgctgt cctcccgctg gatgcggccc tgtatgccga gatcaccaaa gaccgggacg
    10501 aagtgaaacg ctgctgtgag tgcggggcca cgttcacgcc gaagtctaac cgggccaaat
    10561 actgcgcggc ctgctccaaa cggatgcgcc gccgcaaaga ggccgagcgc cagcggaaaa
    10621 gatactggaa acctacgcac ttagagctgt aaaagccctt atatttcaag gcttttccgg
    10681 catggtcagc ggggtatgtg ggtgttttat cctatccccg ccgaagtgct gttctaaatg
    10741 cgtagaattt accagcagaa aagcgccgtt ttctctggtt ccatgcttcc gaatatggta
    10801 tcatagaaaa aatcagtctg acgggggtat caggatggac ggacaagaaa agctgctgga
    10861 ctatgaaacg atcaaagccg ccgttgccgg tgagaggtgg gccacggaaa aagtgttggc
    10921 ccattatgct gactacatca acgagctttc cacggtggag atcagacagg caaacggcaa
    10981 aaagaagaaa gtcattgacg aagatatacg gcagcgcatt tccctgaaac tgctggaagc
    11041 attgccgagc ttcccgctgg aacagagata gacggcaaga gggctgcacc ctcttacgcc
    11101 gctttgcggc tatcacctgc acacccctga aacagataca cccgctgcgc ggctatatct
    11161 gtttcagggg tctaattata gctgcaacct acgcatttaa gggattgaaa accgcccgct
    11221 gcaaggcttc tcccgcatgt ttgcaggggc aagcgaatac ttcatcctat ccccgacaat
    11281 accgggttct aaatgcgtag gaataggcaa aaagaaaaga cgccggacgc aagcgaccac
    11341 atggccgccg cgcccggcgt ctgctgttcg ttattcgtct gtgaagtgga acttctggcc
    11401 gtcatagtcg gcatccgtca tgcgttccag cttggaaagg gtctgctggg cctgcgcccg
    11461 catatcccca tccataacca gcatgacggc ggtgagcctg tttatcgttc tgcgccggtc
    11521 tgcattgtgg tacatacaga tcaggttttc ttcctccacg gtaaagtgca tcattcctta
    11581 tacctccaat tcattctttt tggttctggc cgccgccttt tttgggctgg cggcctgttt
    11641 gctgtcggat agctgcttgc ggatagaggg ccggggcctg cgaatactca catcccggcg
    11701 ctcgtcccgg ttaatcacgg catacacgcc atgcctgcct ttcatcccgg taaaacacag
    11761 ggatttatag ggcagcatgg caaagagccg gtcattatcc ttggtggaag caatcgccgc
    11821 aaagtgcggg gagatctcca ccatgaaatg cgttttattg gggctgttag gcgcggacag
    11881 atctttcagg ctgtccacaa tacgccgggc ctcggtttca atccggctgt catgcaaagc
    11941 ggtgacgtgg actttgaaat cgccggggac ggcccgcccc cggctgtgct gctccgtccg
    12001 tagcgcctcc tgcagcacag ccgggtcatg gggctgggcc tcatagtaca aaaagtttcc
    12061 caatacccgg tcagggcttc cgtcaaaacg ctggctctcc gggatgtcac gggtgccatg
    12121 ctcataaaag agccggttca cactcaccgg ctgggcggtt tcaatgacgt gccggaaatg
    12181 ctgcggatag tccagctcat agagattgcc ccggatagtg ccgccctgtg tgccggtcag
    12241 ctccaccgcg taggccagta tcttgtcacg ggtctgctcc ccgtaaaatt tccatgtgtt
    12301 gtactgtcgg gtgtctttca ggaaaacatc acgttcccgg aaacagtacg tcccggaggg
    12361 ccgggacatc cagagcaggg tcttgtctgc ggcctgatcg ctggcggcat cccgcctgat
    12421 aatgtctttg tcaatgccaa agtcactttg ataaaacgcg gtgttctgcc gcatgatctg
    12481 ttccagcgcg gccagaacgt ccacattttc aaacttctgc atttactcta cctccaatcc
    12541 ctttgatttt tcccgcgccg gtggctgctt ggcctgttcg gtcttggccg cgttgagctg
    12601 cgcccggata gagggctttt ctttctccct gcgggccgca agctgtaagg cgctgggctt
    12661 ttcgccggaa aggggctgga tgcacttgat acggtcagcg acgcggattg tggtgtcgtt
    12721 taactgccgc tgccatgccc cggccttggg cgaccagcgg aaaccgttgc ttttcagctc
    12781 gtcgcgtaca gcagcatccg gcttcccctc aaagaagatt tgcaggcggt tggccacccg
    12841 gttgatctcc acagtgccgc cgtcaaactc ccatcccaca taggccgcct ccttttggcg
    12901 ggtcaattcc tcaatgcggc cctttaaccg gcgtatctcg gcgttattgt tggagagctg
    12961 ccaggactgg aacggttttt tctcatagtg ccagcccgac gccatatccg ctttcaggtt
    13021 ttcaatgcct ctttcggtca gatgggggca tccgtccagc gtcccatgct tgcggtagta
    13081 ggcattgacg gccttcatca actcctgcga cgcttccagc ttttccagtt tcttttccag
    13141 cttggaaacg gcctgcggat cgtcggcgct gataccaccc atgccggtgc tgcggatttt
    13201 gtcaagcaag ccctgaatgt actgaaattc ctccatgttg cggtcactgg cggcgttctg
    13261 cttttctttc gcccgtaccg ggaaattgga cggcccggca atcaggacag aggggacgcg
    13321 ggcggtaatc tcatagccct tattcatgtt ctcggccagc ttgcgggcgt aggtgtcaag
    13381 gaggctgtct atcttttcgt gatactctgg ctccacacgc tttttctggc gctccgcaat
    13441 ttccacggcc tgatcgacat agtgccggta ttccgccgtg gcgctgccgg gccggtagtc
    13501 agaaaagctc atggcttcct tggcgcggcg ggcggctccc tcattgatgg gataatagcg
    13561 gacagccgtt tgcgggggcg cagcctccgg gggttcctcc tgtgtctggc cggaagcaac
    13621 tgccacatcc tcaaaagata gctgttcggg ttcctccggc tgcggttcgg gcggcgtatt
    13681 gttgataatg ccgtcaatca tgttttcgtt ctgctccgta gacagttcgg cggtacgcag
    13741 gggattttcc cgcaaaaact ccggcacttc catatagccg aagctgtcta catagtgggc
    13801 ggtgtcctgt ccattctggt gcagcaccac cacgtcggaa acggaaaggc tgtgtcccct
    13861 aaaatctgcc gggtggtcga tgttgaatgt gcggtaaata tcctccaaag acgtgctttt
    13921 atccagcggt gccgtataaa caaggtcata gttggcactg tccacggcca gccctgcggc
    13981 ctgtaaacgc tcgtatggct caaaccgata gtcgcgggtt tcctccccgc ctcgaagctg
    14041 gtagatggaa aaggtgtcct gttggggtgc ttcatagcca gccgccgcgt attcctccac
    14101 ggtcataccg gctgcggcgg cctcctgcgc gatctcggcc tgtacctgtg ctttgtagtc
    14161 ctgcaagctc tggtcgaagt cagccagttt cggcggctcc ggcaactgat actggccctg
    14221 attgagcatg gggcggcact cctgcacata agagagaagc gcccccaaat aggccgtttc
    14281 ctccggcgtc agggtttccc cggactgtaa gcggccctgc gccgtctgtt ccatttgggc
    14341 aaggctgcaa tgcagcttca tataggggac aaactcgcct aacagcatat cccgatgggc
    14401 cttgtcctgc tcccagccct ctatgccggt tgtagcgatt acatgctttt tccagttttc
    14461 gtcctgcgcg taaaattggt ggtagctctg gatatgttcg atcagggaac cgtccctatc
    14521 tccaaaatcc tgccgcccct cgtagctgtc cggctcgccc tgaaagatac agtcaatgcg
    14581 gaacgcggtt ttgtcatagc ctgcctctgc cggatatgct gcatccagac gggcaaacag
    14641 cgcgtcggcc tcatgcaacg gcatttgctg gccgtccttt agctgggggc tttcgctcca
    14701 tagcacagtc acaagcggct cggctgccgg atcaagggcc ggggcctgtt cttcctcggc
    14761 ctgcgccggg gccgctgcct ccggggtaac gtcaaccgtg acggcctcct gttcctgccg
    14821 ggcctcgcgc tcctgctgaa gctgggcaaa atggccgtcg atctcggtta tcaattcgct
    14881 ggccgtggcc cggatggttt caagggaggc tttcagctcg gataactccc tgccgctgct
    14941 ccaatcggcc acatagccaa aagaatagtc cgacgtgtcc agcccgtaat actggcagac
    15001 ggtataggcc acgctttcgg cctgtacctc gcgggtgctg cggtctggcc gggccgcctg
    15061 ttcctcggcg ggggcctccc ggtcaatggc gtggagcttg gcatgggcaa tctcatggat
    15121 ggcggtcttt agggtctgca attcgctcat gccctcgttg atggcaatgc gctgttttgc
    15181 gtagaagcag cggcccttgg ccccgtctga aatcggctca atgcttatgg gatagggtga
    15241 tgtccgttcc agcgcggcaa agaaatcctg atactgctgc acgtcgccgg taagttcact
    15301 gatatagggc atgggaaaat ccttgccctc ggtctggctg atgtcaaaga ctgtcaccac
    15361 cttaaaggcc ggaaccgtca cttcctgttc ttcggtcacg ggcttgccgt ccgcgccgat
    15421 cacgggctgc tgggtggccg ggtcgatttt ctccatctgc tgcttaacct tgaacggggc
    15481 cggggccagt atcttaatgc ctttctcgcc ccgcttcaca tggcggtcaa attcatcctg
    15541 ccacctgtta tagcccgcca caatcgaacc gccctgcatg gcgatcagca gcgtattgtt
    15601 cagactgtaa tcgtgcatct tggacatagc ggataagtaa gccatatagc ggctactttc
    15661 aaaaagctcc tgtacgccgg tttccagacg gtcagtaatc actttcatgc gttctttgct
    15721 gttcatgccg tccagcatga tcgggataac cgggggcggc gctttgtcgg ggctttcgga
    15781 tgcctccggc tgctgggcct gcggttcctc ccggtcagct tccggctggg ggtagtgcat
    15841 cacccggtat tcctccggca ccggctggcc ctcatggacg cgctgccatt cgtcgccgct
    15901 tctcaccaga tagccgtact gggtaaaaac gccttgttcc tgctgggcaa tgtggcgtcc
    15961 aaactgcgcc gtatcaatcc cggctttcca ttcctccggc atatccacca tgccggaacg
    16021 gttcaggtag tagtcgccca actgtccatc ccccctaacg tcgggaagat gcacatagta
    16081 atccacattt tcggtgtagt cgatgatctg gccgatattc tcaaagccgc cacggggatt
    16141 ttgcagggcg gcgttcagct ctgcgatctc ggcggcgtca agctggccca gccgggcggc
    16201 aaggaaattc agctcgtcca cgtcggcggc cagcaccatg tcatagggaa tggcaaggtg
    16261 tctgtcctcc gggcaggaat acccgttgat aaaaaaatcc tgcggattgt cggcagtaat
    16321 gccaacatcc cgcagggcgg cttgcagcgt ttcggttgtg gtgggcaaat ccagccagac
    16381 gccttgtttg ccctgttcca aaaactgccg gttatggact tggatagaaa atacttcgtt
    16441 cacgcggtca ctcctttctc tgttctgttt caaaaaatag ggggttgggg acttccccaa
    16501 caagcaaaaa tccccggccc ggcagggacg cggccggggg attttaccgg gagtgtagcc
    16561 acactcccta tgcttgctgt gttactgtcc tttagctcct gtgttcaatg cggtagttga
    16621 cgtacttgcc cccggtatca gccaaaagca ccgtcccgtc gtaggttttg ccggtcttta
    16681 cggaatagag gccctttacc ttgactttgc cggttttgag cagcgcagcg gcgatcttcg
    16741 gggtgaacgc tttcttgcgt tcctcaaaaa atcggtcatt cttccacatg acgaactggc
    16801 aggcccggtt tccgcagtag tagtttttct tgccctcata gacagcctca ccacagcggg
    16861 gacacgtccc gataggaaca cgctcggcct gaaacaattt ctgcttttcc tcactgatac
    16921 aggaataggt ctgcaccagc tcccgcgcct gcgtctcgat gccgtccata aattcgtcag
    16981 gatcggcctg ccccttggcg atctcggtca gccggttttc ccattcggca gtaagctggg
    17041 gagaagtaag cgcctccggc agcaccacag ccagattgat gccgtccttg gtgggaatga
    17101 gctgcttgcc ttttctctgg acaaagccca tctgcaccag cttttccaga atggcggcgc
    17161 gggtggccgg tgtgcctaat cccttgcgct cggcatcctc cggcatatcc tcggccccgg
    17221 cccgttccat agcggacaaa agcgtatctt cggtgtaggg cttgggcggc gtggtgaaat
    17281 gctcggtgac agaggcggcc acgtctgcaa aggtctggcc ctcgcaaagc tccggcagct
    17341 cccgcaccgc ctcggcgtcc tcgtcggcgt cggtctttaa ggaagaacgg aaccggcgtt
    17401 caatctcttt ccagccgggg gaaaggacgg tcttgccctt ggcggtaaac tcctgcccgg
    17461 cacaggtaaa tgtggcggtg acggcctcat agatatgcgg cccggccacg gcacataaga
    17521 gcttgcagca gatcagggac agcagctttt tttcactctc ggccagagtg ccaaagccct
    17581 gcttcacaaa ttcagcggtg gggataatgg cgtggtggtc ggacaccttt ttgctgttca
    17641 ggatgcggtc aaactgcggg gaaaggttca ggcccttggc aaacggcacc agctcccaca
    17701 ggccggacac aaggctttcc acggcgggca gcatatcgtc ggtaagatat tgactatccg
    17761 ttctcggata ggtcagcagc ttctttagat acagtgcctg ggcatagtca agggtctgct
    17821 tggcggtata gccgaaaagc cggttagcct cccgctgcaa ggtggtaagg tcatagagct
    17881 tgggcggctg ttccgtcttt ttctcccgcg tcacggaaac gcaaacggcc tgcgcttttg
    17941 cacaagccgc cttgacggtg tttgcctcgg cagcgtcgga aaagcggctg cttaccgcgt
    18001 ccgcgccgcc cgccgtgata tggacgatgt gatatttctc tttcttgaaa tcggtaatct
    18061 tggcgtccct gtccaccagc atttttaagg tcggcgtctg gacgcggccc acagtcaggt
    18121 tcttgtggta caggacggaa aaaagccggg tggcgttaat gccgatcagc cagtcggcct
    18181 tggcccggca cagggccgat tgatacagcg gatcatagtc gccgcccggt ttcaggtggg
    18241 caaagccgtc cttaatagca ctgtcctcca tagaggaaat ccaaaggcgc tcaaagggct
    18301 tttggcatcc cgccatctgg tagacgaaac ggaaaatcaa ttccccctcg cgcccggcgt
    18361 cggtggcgca cacaaggccg gtcacgtcgg ggcgctgcat cagcgaacag agaacgtcaa
    18421 actgctgttt cttgtcgtcg gggacgatgt actgccatgt ttccgggaga atgggcaaat
    18481 cctcatagcg ccattttttg aaacgctcgt catagcgtcc ggcgtcggca aaggacacca
    18541 gatgtccgat gcaccagctc accagatagc cgttcccctc caaatagccg tcgtgtttct
    18601 ccgtggcccc gatcacgccc gcaatgctgc gggccacgct gggtttttcc gcgattacaa
    18661 gtttatatgt cattcctcgt cctcctgttc actcacaaaa tcagcttccg ggtcaaactc
    18721 ggtttcgggt tcctcgtcct ccaaatcctc gtcctcccca aaatcatact cgcccaaatc
    18781 gtcgctgccg gtggtttcag ccttgggctt tttgaacttg aaatagtaaa gggccgcacc
    18841 gccgcccgcc agcgccacca gcaggaacac gatcaggccg cccgcgccgc ccttgggttc
    18901 ttcctccggg gcttcgggtt cctccggctg gggttcctgc ggctccgggc cgctgcactc
    18961 ggtcaggttg gttttacata ccgggcaatt cgtgttcaca gccccggcct tgcatttcgt
    19021 ggtacaggag cagacagcag cgggggtgac ggtttccccg tcctccatca gcgtcagcag
    19081 atcggcttca tccacctgat ttaagaaatg gacagtttct tttccctcgt cgtcccggtc
    19141 aataatcaga taaaacacat tgccgttttt ggtttccacg gtgataaact gcttgccatt
    19201 tttcgcggcg ctgccgatgt cgtcaatcag tgacaggttc ccgtccgggg acagggcggc
    19261 gctggccccg ccgccattga acagttccag cagagaagca atttcctccg ggtcaatgga
    19321 gagggaaaaa ccgccctcgc cggtagccgc tacttccttt tcgctgacgg tcatatagtc
    19381 ggaatggaca tagccgatgc gctccggcaa gagaatttta atccagtcgc cgtctgtgcc
    19441 gactacctca accgtcgtcc cattgggaag ctgggtgaac gcggtcttgt caagccccgc
    19501 gccggtgcgg acattcagcc tgccgccgtt ggtggtgacg gtgccggtct gttcaggttc
    19561 ttttttgtcg gtgccggtgg tgatctggat gccgtcgtcc gtggcggcaa tgctcacctt
    19621 ggaaccgaac agcgcggaaa acaggtcggt gatgtccttg ccggtcagag ggccggtatc
    19681 tttttcggcc tcggtttctg tcccgtccgt ggcggtgccg gtttcgtctg tttcaggctc
    19741 caaaccgccc gtagcgggtt cggttgtagc ctccggcagc ggggccggtg ccgggtcatt
    19801 gccgcctgca agggcggtaa cgggaaaggc cgtgacgcag agcatcacgg ccagaagtgc
    19861 ggggagaata cgggaaatcc gtctggtctt attcttcatg ggcattgtcc tcctgttcat
    19921 ggtaaggggt tgcggcaggg gcggcctgca ttgcgccgct gcgtaagaac gcggcaaatt
    19981 cctccggctt catgttcatg ccgcgcacaa gctggatgat ctgcaaattc tcctgttcgg
    20041 ttttctgcgt ttccagctcc cgaagctgtt tctgaaactc cgcgatctta gacttggttt
    20101 tctggatgtc cttttcgatg cggtcaagtt tggttgccat aattgaaagc tcctttctgg
    20161 cgttaaggta aacgcccgta actgtaaaaa tgggattgcc agtagtttga agtaaggttc
    20221 gtgtaggaaa tcgggttccc gcaatgcagc atcacattgt tgcccacata caggccgacg
    20281 tgggacacgc cgggggtgtc gtaggttccc ttgaaaaata caaggtcgcc gggcttggcc
    20341 tgcgaactgg aaacaggggt gcagatgtta tataggccct gcgcccccag ccgtcccacg
    20401 ttccatccac tatgattgat tacccacgaa atgaagccgc tgcaatcaaa gctggtgctg
    20461 ggagagctgc cgccccacac atagggataa ccgatatatt tttccgcctc tttgagcatg
    20521 gcggcaaagg tggcatcctc caaagcctcc gggggtacgt cgtagtaggt cggcggcgtc
    20581 agggtggagg cgttggggta ctggctgccg gaaaacagat caggccggtt tcctagcgtg
    20641 gacatataca gggaatagaa acttagcgtt tcctcgtcca tgatatagac tggcacatgg
    20701 gacaggtcga agttttccag cgtgacgtag cagatgtaat agtcatacgg cacttggtag
    20761 gtgtctgtat gggtgttgcc ctccgcgtcc gtccatgtat cggtttcggt gcggtaacgg
    20821 gtttctacca cgacgttttc tgtcaggata tattgcttgt caaagagcat ggacaaatca
    20881 ccctgtacct ccccgatggt aaatatcccg tcatggagcg ccgacagaat ggaaatcagc
    20941 acataggggt catggctgat ctcgtcaagg tcaaaatggt attcgtcata gtcatgggtg
    21001 gcctcgtagt tgtccagata gtcttgcagc tcggcttcca gctccgcgta ggcgtcctcg
    21061 gcggccaaca tatcgctatc ctcggaaaag taagaggacg ccgtgatgcc gcttccggcg
    21121 ctgccgaaca tggcggtgca ggactgtaag ccgccgatga taagcagcat caaaagcccc
    21181 aacacaagga cggccagcgc ccctttccag tggcgggcca caaaggaggc cgcccgttca
    21241 ctttctttgg ctgcggcctt ggccgctttc gtggaggccc cggctgtctt ggccgcgccc
    21301 tgtgccgccg cgcctgcggt ctggcccgct gtccgggcgg ccttggcgta ctggcgcttg
    21361 atctgctgtt tctgccagaa ccgggaaatg gggttgctgg ccgcctgtgc gatctccggg
    21421 ttatcccgca aagatttctg gtagaaatac tccgcgttgg cagacaatag cctgcgctcg
    21481 gccttttccg cgtcccagta gggtttcagc ctgtggtgcc ggatggtgct gcggatggct
    21541 tttccggcct gccgctcggc aagttcctcc cccttatggc cgccctccac acccacgttt
    21601 tcatgctcga cttcatggat tttcccgtgg ccgaatagaa aaacctcgga aacaggacgg
    21661 cctgccggat tgggtttcag ggagggaggc cccttgtcct gtttgtcaaa gtgcaggacg
    21721 gtctttgctt tgccgcttgc ctcgtcaaag acggtttcct tgaccgggac gcgcttcttg
    21781 gggatggcgg cgcgggcctt atccagctta tcggcccgtt tgtccgattt tcggatatag
    21841 ggctgtaaat ccgggtcggc ccgttcttcc tccgacagtt ggagccgggc ggatggccgg
    21901 tgcagggcag cgtccccctc ggcggccaca gccccggcct gcttcgcggc tttcttggcc
    21961 ttatggccgt catggatggc gtcggcccgt tccagcagct tatcggcggt gccgcccggc
    22021 cccggcgcgg acaaatcctg ttcggcctcc cggctggaaa tgttctccac ctcgccggtg
    22081 gcctgatttt ccagcgtcag cccgtcgcgg gtcatggcct gcgtcacttt cgccttggct
    22141 ttcagctctt tcatggtggt tcacctcctt tgtggcgtcg ccgggtcata ctcccgccac
    22201 ctcgtttaag cgggtcgtca taatcttgta aagctgggtg ttgtgcggga tggggttggt
    22261 aaagggcagc accacgtttt caaagatcaa aaggccctcg ccgggttcgg aattgtcgat
    22321 gtacttctgc tgttcggtgg acaaattcaa gcgttccgca aggatttttc tatccccgga
    22381 cgcctgattg agcagcgtaa tgaagtcgct gttttcaagg atattttcaa tttccgggga
    22441 ggaaagcagg tctttgacgt tctgggtggc acctgttgga atgccgcccc atttcctaaa
    22501 ccgcttccaa atctccgcag aataggccgc cgtctgttcc tctttcaaaa gcaaatggaa
    22561 ctcgtcggca aaataccatg tggcctttcc ttggcttcgg ttggccgtga cgcgccccca
    22621 tatctggtct tggacaatga gcatccccag ctttttgagc tgcttgccca gctccttaat
    22681 gtcaaaggac acaagccggt tgccgatgtc cacgttggta cggtggttaa atacgttcag
    22741 ggagccggac acatacaggt caagggcctg cgccacccgg tcagcctccg ggacatgctg
    22801 atctaacaac gctttgtgga ggtcggacag gatcggcata ttctccgggc gggggtctgc
    22861 cagatagggc cggtagatca cgcgcacagc ccggtctatc acggtctttt caatggcttc
    22921 cagcccgttc ttgccgccca tgacaagctc acagaacgac agcacaaagt cggatttcag
    22981 ggccagcggg ttatcgtcct ccgaataatt caggttgatg tccagcgggt tcacaaagtc
    23041 cttgctggta ggcgacagcc ggattacctg tccgtggagc cgtttgacaa gggggtaata
    23101 ttcggcctcc gggtctgaaa taaaaatatc gtcaggcgtc gttaagaaca cactgacgat
    23161 ctcggattta cagctcatgg atttgccgct gccgggggtg ccgagcttca aggcattggg
    23221 gcaacgggcc tgcttcctgt ccagcataat catgttgcgg gacttggcgt tgatgccgta
    23281 atacatggcc gcgccgccct gaaaaagctc ctgcgtcaca aaggggacga acacggccac
    23341 gctggaagtc gtaaggctgc gctgtatctt gatctgattg acgcccagcg gcaggctgct
    23401 tgcaaggccc tgttcctgct ggtagtccag ccggacaagg ggacagttat gcttttgggc
    23461 cacgcctgcg gcttggaaca cgtcgttatt cagcttttgc ttggtgtcgg ccacgttgag
    23521 gacaaggaac gtcaactgga acagccgttc attgcgggtc tgcaactttg tcagcagctt
    23581 cttggcgtcc tcgccgtagg tggcaaggtc gctgggaaga atgtccatat cgtaaccgct
    23641 gcggacggcc tttttctgct ccgcaatctt catagcgtca aggtcggtaa ttttgcgctt
    23701 gatggtcttg atcgccttgg tctgttcaat ggcctgcaca tggagattga ctaaaacacc
    23761 gctttccgta ttgaggaagt cggccagcat atcgtccgac agctccgggg acaggatttg
    23821 caggaaactt accgcgccgt atttgccgcc catgccgaat gtctttgcat tgccgaagca
    23881 cagggaggac ggggcaataa aatccttggt ggagaggccg gacggggcca gccacttcca
    23941 gtcaaaattg aatatctcgc cgtccgggtg gtacacgcca tgcagcaggt tcagccgttc
    24001 ccagccgtcc atcacatggg ctacggcccc catgctcttg aaatagccta acaggtcaaa
    24061 gccgatgcgc ttcaatctgg ctcgcgccgc tttcacgctt tcggcctcga tggtgaatgt
    24121 caggtatttg gtctttacca tgccgttgtt gccgtttgca agctgctttt gcagaatgcc
    24181 ggtgtactcg gcccggatgt cgtcgaagtc gtccccctgc ggcgcgatct caaagctctt
    24241 ggcgtactgc accgggtcaa ctttccgatt gaggaaagaa aactggatat gaatggaggc
    24301 gtccacatag ttatacaggt cgcacagttt ttcaaagata gccgtctgcg tgtccggctg
    24361 ggcaagctga tagctgatgt ccgcaaactc aatgcacttg gaaaagacgc cgggcgatac
    24421 ccggcacacg ccgtccgggt acatgacctc gtaggggatg ctctgctgcg ccgtgtgggg
    24481 cctgccgtcg cccttatatt tacggattac cgcgtcaatt tcttttcgct gggcgcgggt
    24541 cagctttgtt ttttcggttt ctcttgggat ttcctttttg gacaatggcc tttacctcct
    24601 gttccatctg gtattgccgc acaagggcgg tgtaggtgtt gtcggtctga tagggacgct
    24661 cctttggctg gatgaagcag cactggatga tctgcataat caccacttca aggggctggc
    24721 cgtgacgctc gtacatggca agcaaaaagc ccggcagcat ggcaaatacc atgagcaggg
    24781 ccgccgcgct gttggtaaga tagtcacggg tgagaaaata taaggggacg ccgatcacca
    24841 gcgccccgga aaaacagatc agttgccgtt tggtaagccc aaacacaacc ttgcttttca
    24901 catgggttaa gtccttgggg acggttacat aagccatttg gcacctcctt tcacgtcagg
    24961 ataaggtcac attccacctc gttcccccac acgtcccagc cgggggtttt ctgtctggca
    25021 aacagctcca cgcggggcaa atcgcccgcc agctctacga ttttttcacg ggtcacatcc
    25081 ggctttttgc tgtgttgttc aatcgggctg ataataaact gatggacaga ggcagaacga
    25141 cgcttcggct tgccgtgtgt tgccagcagg cagatttccg cgtttcccct tgtccagcgg
    25201 cccagcccgt aaaaccatgt aaggctttta cggttctgtt tcagccatac aaaagccacg
    25261 gtcttgaatg aaaagcccca cgctttgatt aaccgcagag cttccggcag catggggaat
    25321 gtggcccaca aaaacagcag gcagtctttg gacgccagcc gttccaccgg cagggcgcac
    25381 agctcgtcaa tgctcatggt ggaatagtga ttttccgctg cgccctgtac gtttttgcag
    25441 tcataccgcc acggcgggtc agcgtaaatg atgttgtacg ttgaaataag caagttcctc
    25501 ctgtgtatgc ttggattttg ggatcaatgc gccccgaata cggcctttga tatactgccg
    25561 gtcttgaaca ggcagaagca cagcagcacc gtatagccca tacagcccca gattgcgccg
    25621 gatatgtcgg aagcggaacc gatattctgt atcagcaccg cgtagatacc caccacaacc
    25681 atgattaaaa aggcttggaa gcccagcgcc agcagggatt tcagataccc ttggcccatg
    25741 ccgcgccatt ccgcgttgcc cattgtggca agggggatag gccccagcga cgtgacaaca
    25801 tagatttcca ccatgcggcc ataggtcaca aggaaaatgc agatggaaag gatattcatg
    25861 gtgaggccca caacaagggt ttggaaccac agccccaaaa gagcgcccac gctcatagcc
    25921 tccaattggc tttcaaggtc tggtatcagg gtgttaaaat caatggatgt ctgcccgata
    25981 atcacaccgg cgctttgatt gacgacgctt tgggtggcgt cgaatacgcc catgacgatg
    26041 ttccatgtgt tcgttacgat caggattgca aacgcggatt tgaatatcca gcggaagaac
    26101 atggagctgt caaagtcgtg catattgttc ttttccgtta tcatctggat aagctcatag
    26161 cacatgacaa gggcgagtat ggccccggct atggggacga tcacggtttc cgacaggctg
    26221 tgcagcatat tgaaaatacc gctgttccat gcctgcgggg ttcgtcccac gtcggcggcg
    26281 atttctccga ctttggcatt taccgtatcg aacagcccgg acaggttgcc cgtgataccg
    26341 tccatgagga tgccttttat ccattcttca atgatttcac taatcacggg tagcacctcc
    26401 tttcccgcgc cctcccggtc tggccgggag gacgcggagg cgattagtta agggtttgga
    26461 cagggacaaa ggccaggatc agcccagcag cccggacagc aggggaacca gagtgacacc
    26521 gatcagggcc acgccgccgc cagcgacaag ctgcttgaca ccctgcgatt ttgcccccgg
    26581 attgtcctgc ccgtatccct ccaacagatt gatgccgccc catacgcaca gagcgccgcc
    26641 cagtccgatc acgatagtct gcaaagtagt aactgcggaa ttaaaaaatg ccataaattt
    26701 acctctttct gccggtttcc ggctcataat gattttttga ttttgttgtt ggaatgaaaa
    26761 aagccgctgt ttattcagcg gcggcgtcct cgtccgacag gtcgatttca tacaggtcga
    26821 atacttcatc ctgcgttacc acttgctgcc gtttccggcg cgtgatagtc aaaaatctgt
    26881 ccacgtcaaa ggtattcttc ttgtcggcgt cggcaaggta tttgtaccgg ggatgcttgg
    26941 taatatcgaa tttctccgat aaaaacgggc gcacaccccg tagctgcaag atacacttgc
    27001 cgccgtccat cacggcaatt tcgtcttggg acatcaactc ctttcctaat ttttgatagt
    27061 tcaatccgtg ggacgtttgg cttccccggt tctcgctggt gttgaaactg tcaatcgttt
    27121 ctttccccag cgcatcggaa atctccttgg catttttgcc gcgcccacta aggaaaagca
    27181 cactatcgca gttatccgaa atgatctcgg cggcgtcctt gtaaatcgct tttagctgcg
    27241 actggctctg caaaataata gaggccgata tttcacggct tcgaatggtg gcaatgagtt
    27301 tatcgaaatt gggtatttgg ccaatgttgg caaattcatc caaaatcagg cgtacatgga
    27361 caggcagccg cccgccgtac acatcatcag cccggtcaca aagcaggttg attagctggc
    27421 tttggagcat agccagaatg aaattaaagg tcgtgtccgt atcgctcata atgagataca
    27481 gcaccgtctt ttcgtccccg atggtgtcca gctccatttc gtcgtcctcc atcagctcgc
    27541 gtacctcccg gatgtcaaag ggcgcaagtc tggcaccgca tgaaatcagg atggaagaac
    27601 gggtcttgcc tgcggacagc aggaatttct tgtactggcg caccgcaaaa tggtcggggt
    27661 ctttctgctc caaacgctca aacatgagat cgacggggct ttgaaactcc gggtcgtcct
    27721 cgcgggcttc actggcgttt atcatttcca gcagcgttga gaagttcatt tcctccgctg
    27781 gggcctcgta ccagatatag ccgatcaggg ccgaataaaa caaccgttcc gacttcaccc
    27841 aaaaatcctc cgctgacttt tccccctcgc ccttggtgtt ggaaatcagg gtatttacca
    27901 gctttagcac gtccttttcc gaatggatgt agacaaaggg gttgtacttc atggacttgg
    27961 aaaagttaat ggtattcagg attttaatgc ggtagcccgc ctgctggaac atcctgccgg
    28021 tttccagaac caaagtgcct ttcggatctg tcaccacaaa cgaagtcgga tagtccgtgg
    28081 tcggaaagca ttgcatcagg ttgggtttca cgaaaaatct cgttttccct gaaccgctgc
    28141 caccgatcac cagcacgttt ttattccgcg ctgtcttggg gtctttgggc ctgccggtca
    28201 tggtaagccg ttccgtttgg gtgagcagga tattattttc aaacttgggg tcgatgtaag
    28261 gcgctatgtc tttggccgtc ccccagcggg cgctgccata ctccatgcct ttgcggtatt
    28321 tcttcacgtt cttgctcttg cagtacacca ccagccgcac gatcacggca ccggcgatac
    28381 cgatacacag gtcaaacagg tgcaggcttg gcagcgggtt ggcaaaagcc gccgcaaaac
    28441 catccgcaaa atgcaggagc ttccccgaca ggtcagcacc agcggccagc cggtacgcct
    28501 ccccgcattt cgcaaacagg tacacaaaaa tcagatacgg gaggttcagg atcagcaatt
    28561 tctttacgtc aggcttcaca gttccagccc cctttccttg attttctcct tggcccgctg
    28621 cttggtctgg gccgccgcct tttgcttcac agcggacaaa accttgcgga tagagggccg
    28681 ctgctccttg gaaagcgtct tggcggtaaa ctcccgaaac gccagatcaa ggttatctgc
    28741 atccttggcc ttgaaaatca caacgtagtg gggcggctgg gtggtcttat cttttcgtaa
    28801 ggtaaaatcc acgccgtatt tcttggcgca tggcttgaac gctccaatgt tggcctcggt
    28861 gatctcaatg ttggagagat cagccccatg ctttttaagc tgctttaggc tctgctggcc
    28921 gtggtgcagc gtgtttttgc ccttggcccg gtgttgcagg tactttttca gcgccgcttg
    28981 cagcacttga gccgtgagct tgcccgtttt catggacagg gcaatggtct tttgagtaac
    29041 ttcttcctgc aatcgcatcc ctccttgtca gaaagtcaaa agcggcgtct aaggcggaac
    29101 caccagacgc cgcaggtaga tataggtcaa atccgcaccc gcaggccgga aaggtcgtcg
    29161 gcccggatgc cgacaagata ccagttttcg gccatttcaa tgcgggtggg cctccatttg
    29221 ccgccgatca tcacgtcgaa acactccccg caatgcaggc cgccgtaata gtcggccaga
    29281 tcaaagcgga tgtcgtaacg gtctgttcgc tcgtcaaaaa tcaatgcgcc ctgcttcata
    29341 gggtcgtcct ccttttctcg ttacaggctt tcgccctcgc cgcaggaata gtccggcagg
    29401 ccggatttag gctttcccca atcgggctgt gccatgtcgt gcgcgacaag ggccgtgtag
    29461 tagttggaaa tggtgctggg ggcgttgaac agcatagccc gcaggtattg tttgatattg
    29521 cggactttcg ttgtgttctc ccgcagacag tcaaggacaa agcggatatg ttcgccggtc
    29581 agcttcatgt acttggattt caccagctcg gcagggtagt cgtccccggc gacacggatt
    29641 gtctttcggg cggtgcagac ggtttccagc atcaggtcta cgatttcgtc aagctgttca
    29701 cggtcaatgc tcctgtcctg aatgagatag gcatactcga tgttctccaa aatgatttcc
    29761 cgataaatcc gtacagcctc atcctttcct gtccttttcc gttccggcgg cgtagccgct
    29821 gcctcgccct ccataggggc aggggtcggg gatgggatag gaatggaatg ggtactttgt
    29881 ccatctgtaa ttattttttc tttttttgat aagtcagttc tttgtacttc tttatttaat
    29941 tgcgttggat tttccgacgc cggattatcc gatgtcggat tttccaacac cgggttatcc
    30001 gatgttggat tttccaatac cgggttttcc aataccggct gttcgtagat ggtatagacc
    30061 atatcggcca ttttgccttt tgcgtcacgg ccctgctcac gccggatata tccagctttt
    30121 tcaagctccc ataccgctgt cctgatcgcg tcgatgcttt cccggttgat ataggacagt
    30181 ccttttaggg tatagtccca atcctccggc aaggacaaca tctgcgacag caggcccttg
    30241 gcctttaagg tcaggttctt attccgcagg tggtgatttg acatcaccgt atagcctttg
    30301 ttcttttcca cacggaaaac tgccatagcg ttgttcgctc ctttcactgt gaatttgacc
    30361 gctgcatagt cggtcagctt gcccggctga tagggacact ctgcaaactg gcagaattga
    30421 tatttccaat ccgggcggta aaatcggcag gtgccgcaat cctccggcgc gtccgccccg
    30481 ccgccgtcgt agtgatcgaa accgggcttt tcctgcatca gccgttcaaa cgcccggtca
    30541 gggccggaag taaatagcat ggcgtcgcct cctttctcta tttgggcata aaaaagcggc
    30601 gtcctgttct cccatgacgg gaaagaggaa cgccgcgttt acggtgtcgt attcaattta
    30661 tgatgtgccg tatttggggt ggcgggtatt agcagtatgg ttcagcatat atgcggctca
    30721 atccgaaagt agtaaattgg tagtaaattg agttctcata tcctgcgaaa tgcccgtatt
    30781 tccggggctt tcagcgataa cctaaatttt tggaataaaa ctttcattat aggctactgc
    30841 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    30901 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    30961 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    31021 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    31081 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    31141 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    31201 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    31261 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    31321 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    31381 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    31441 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    31501 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    31561 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    31621 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    31681 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    31741 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    31801 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    31861 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    31921 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    31981 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    32041 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    32101 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    32161 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    32221 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    32281 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    32341 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    32401 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    32461 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    32521 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    32581 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    32641 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    32701 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    32761 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    32821 taccagccga aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc
    32881 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    32941 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    33001 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    33061 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    33121 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    33181 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    33241 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    33301 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    33361 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    33421 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    33481 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    33541 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    33601 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    33661 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    33721 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa
    33781 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    33841 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    33901 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    33961 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    34021 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    34081 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    34141 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    34201 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    34261 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    34321 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt
    34381 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    34441 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    34501 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    34561 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    34621 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    34681 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    34741 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    34801 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    34861 gcggtagcgt gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg
    34921 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    34981 gcacatgggc tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg
    35041 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    35101 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    35161 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    35221 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    35281 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    35341 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    35401 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    35461 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    35521 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    35581 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    35641 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    35701 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    35761 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    35821 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    35881 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    35941 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    36001 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    36061 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    36121 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    36181 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    36241 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    36301 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    36361 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    36421 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    36481 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    36541 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    36601 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    36661 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    36721 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    36781 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    36841 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    36901 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    36961 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    37021 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    37081 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    37141 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    37201 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    37261 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    37321 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    37381 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    37441 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    37501 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    37561 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    37621 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    37681 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    37741 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    37801 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    37861 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    37921 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    37981 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    38041 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    38101 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    38161 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    38221 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    38281 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    38341 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    38401 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    38461 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    38521 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    38581 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    38641 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    38701 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    38761 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    38821 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    38881 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    38941 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    39001 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    39061 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    39121 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    39181 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    39241 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    39301 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    39361 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    39421 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    39481 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    39541 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    39601 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    39661 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    39721 aatcagaaac cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca
    39781 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    39841 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    39901 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    39961 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    40021 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    40081 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    40141 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    40201 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    40261 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    40321 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    40381 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    40441 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    40501 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    40561 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    40621 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    40681 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    40741 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    40801 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    40861 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    40921 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    40981 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    41041 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    41101 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    41161 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    41221 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    41281 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    41341 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    41401 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    41461 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    41521 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    41581 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    41641 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    41701 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    41761 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    41821 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    41881 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    41941 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    42001 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    42061 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    42121 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    42181 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    42241 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    42301 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    42361 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    42421 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    42481 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    42541 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    42601 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    42661 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    42721 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    42781 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    42841 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    42901 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    42961 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    43021 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    43081 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    43141 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    43201 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    43261 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    43321 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    43381 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    43441 ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    43501 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    43561 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    43621 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    43681 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    43741 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    43801 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    43861 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    43921 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    43981 ccgtccattt cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc
    44041 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    44101 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    44161 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    44221 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    44281 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    44341 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    44401 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    44461 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    44521 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    44581 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    44641 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    44701 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    44761 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    44821 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    44881 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    44941 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    45001 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    45061 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    45121 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    45181 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    45241 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    45301 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    45361 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    45421 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    45481 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    45541 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    45601 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    45661 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    45721 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    45781 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    45841 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    45901 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    45961 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    46021 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    46081 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    46141 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    46201 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    46261 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    46321 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    46381 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    46441 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    46501 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    46561 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    46621 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    46681 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    46741 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    46801 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    46861 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    46921 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    46981 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    47041 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    47101 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    47161 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    47221 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    47281 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    47341 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    47401 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    47461 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    47521 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    47581 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
    47641 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    47701 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    47761 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    47821 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    47881 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    47941 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    48001 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    48061 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
    48121 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
    48181 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
    48241 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
    48301 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
    48361 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
    48421 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
    48481 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
    48541 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
    48601 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
    48661 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
    48721 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
    48781 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
    48841 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
    48901 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
    48961 gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat
    49021 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
    49081 tttacctttt atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc
    49141 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
    49201 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
    49261 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
    49321 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
    49381 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
    49441 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
    49501 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
    49561 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
    49621 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
    49681 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
    49741 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct
    49801 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
    49861 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
    49921 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
    49981 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
    50041 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
    50101 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
    50161 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
    50221 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
    50281 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
    50341 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
    50401 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
    50461 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
    50521 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
    50581 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
    50641 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
    50701 ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
    50761 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
    50821 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
    50881 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
    50941 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
    51001 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
    51061 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
    51121 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
    51181 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
    51241 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
    51301 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
    51361 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
    51421 tgaatacatt ataatgatac tgtaaatata tctttattat tagtgaatgg atatttactt
    51481 ttaatagata aaatattata actattaaaa ataactattt tcattataat tacaca
//
LOCUS       DAZBWR010000007        46045 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000007 DAZBWR010000000
VERSION     DAZBWR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.007"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(224..592)
                     /locus_tag="ACHGT5_000386"
     CDS             complement(224..592)
                     /locus_tag="ACHGT5_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN7216404.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            742..1224
                     /locus_tag="ACHGT5_000387"
     CDS             742..1224
                     /locus_tag="ACHGT5_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN7216405.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1261..2271
                     /locus_tag="ACHGT5_000388"
     CDS             1261..2271
                     /locus_tag="ACHGT5_000388"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN7216406.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2386..3099)
                     /locus_tag="ACHGT5_000389"
     CDS             complement(2386..3099)
                     /locus_tag="ACHGT5_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216407.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3272..3879)
                     /locus_tag="ACHGT5_000390"
                     /pseudo
     CDS             complement(3272..3879)
                     /locus_tag="ACHGT5_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4363..4590
                     /locus_tag="ACHGT5_000391"
     CDS             4363..4590
                     /locus_tag="ACHGT5_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN7216408.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4606..5772
                     /gene="hemW"
                     /locus_tag="ACHGT5_000392"
     CDS             4606..5772
                     /gene="hemW"
                     /locus_tag="ACHGT5_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN7216409.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6006..7049
                     /gene="hrcA"
                     /locus_tag="ACHGT5_000393"
     CDS             6006..7049
                     /gene="hrcA"
                     /locus_tag="ACHGT5_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN7216410.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7143..7706
                     /gene="grpE"
                     /locus_tag="ACHGT5_000394"
     CDS             7143..7706
                     /gene="grpE"
                     /locus_tag="ACHGT5_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN7216411.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7757..9586
                     /gene="dnaK"
                     /locus_tag="ACHGT5_000395"
     CDS             7757..9586
                     /gene="dnaK"
                     /locus_tag="ACHGT5_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN7216412.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9737..10903
                     /gene="dnaJ"
                     /locus_tag="ACHGT5_000396"
     CDS             9737..10903
                     /gene="dnaJ"
                     /locus_tag="ACHGT5_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN7216413.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10985..11614)
                     /locus_tag="ACHGT5_000397"
     CDS             complement(10985..11614)
                     /locus_tag="ACHGT5_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216414.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11626..11799
                     /locus_tag="ACHGT5_000398"
     CDS             11626..11799
                     /locus_tag="ACHGT5_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216415.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11844..12581)
                     /locus_tag="ACHGT5_000399"
     CDS             complement(11844..12581)
                     /locus_tag="ACHGT5_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN7216416.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12583..14085)
                     /locus_tag="ACHGT5_000400"
     CDS             complement(12583..14085)
                     /locus_tag="ACHGT5_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN7216417.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14344..16338
                     /gene="uvrB"
                     /locus_tag="ACHGT5_000401"
     CDS             14344..16338
                     /gene="uvrB"
                     /locus_tag="ACHGT5_000401"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN7216418.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16348..19164
                     /gene="uvrA"
                     /locus_tag="ACHGT5_000402"
     CDS             16348..19164
                     /gene="uvrA"
                     /locus_tag="ACHGT5_000402"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN7216419.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19427..20692
                     /locus_tag="ACHGT5_000403"
     CDS             19427..20692
                     /locus_tag="ACHGT5_000403"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN7216420.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20696..21427
                     /locus_tag="ACHGT5_000404"
     CDS             20696..21427
                     /locus_tag="ACHGT5_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN7216421.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21562..22446
                     /gene="rapZ"
                     /locus_tag="ACHGT5_000405"
     CDS             21562..22446
                     /gene="rapZ"
                     /locus_tag="ACHGT5_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN7216422.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22443..23441
                     /gene="yvcK"
                     /locus_tag="ACHGT5_000406"
     CDS             22443..23441
                     /gene="yvcK"
                     /locus_tag="ACHGT5_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN7216423.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23475..24410
                     /gene="whiA"
                     /locus_tag="ACHGT5_000407"
     CDS             23475..24410
                     /gene="whiA"
                     /locus_tag="ACHGT5_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN7216424.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24978..25634
                     /locus_tag="ACHGT5_000408"
     CDS             24978..25634
                     /locus_tag="ACHGT5_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216425.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25813..26378
                     /locus_tag="ACHGT5_000409"
                     /pseudo
     CDS             25813..26378
                     /locus_tag="ACHGT5_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27077..27598)
                     /locus_tag="ACHGT5_000410"
     CDS             complement(27077..27598)
                     /locus_tag="ACHGT5_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN7216426.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27796..31476
                     /gene="nifJ"
                     /locus_tag="ACHGT5_000411"
     CDS             27796..31476
                     /gene="nifJ"
                     /locus_tag="ACHGT5_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN7216427.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            31665..32552
                     /gene="cdaA"
                     /locus_tag="ACHGT5_000412"
     CDS             31665..32552
                     /gene="cdaA"
                     /locus_tag="ACHGT5_000412"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN7216428.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            32549..33718
                     /locus_tag="ACHGT5_000413"
     CDS             32549..33718
                     /locus_tag="ACHGT5_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN7216429.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            33742..35097
                     /gene="glmM"
                     /locus_tag="ACHGT5_000414"
     CDS             33742..35097
                     /gene="glmM"
                     /locus_tag="ACHGT5_000414"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN7216430.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(35169..36974)
                     /gene="glmS"
                     /locus_tag="ACHGT5_000415"
     CDS             complement(35169..36974)
                     /gene="glmS"
                     /locus_tag="ACHGT5_000415"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN7216431.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            37384..37674
                     /locus_tag="ACHGT5_000416"
     CDS             37384..37674
                     /locus_tag="ACHGT5_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216432.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            37687..38712
                     /gene="sppA"
                     /locus_tag="ACHGT5_000417"
     CDS             37687..38712
                     /gene="sppA"
                     /locus_tag="ACHGT5_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN7216433.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            38715..39374
                     /locus_tag="ACHGT5_000418"
     CDS             38715..39374
                     /locus_tag="ACHGT5_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN7216434.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            39531..40463
                     /locus_tag="ACHGT5_000419"
     CDS             39531..40463
                     /locus_tag="ACHGT5_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216435.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            40617..41906
                     /gene="tig"
                     /locus_tag="ACHGT5_000420"
     CDS             40617..41906
                     /gene="tig"
                     /locus_tag="ACHGT5_000420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN7216436.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            42153..43403
                     /gene="clpX"
                     /locus_tag="ACHGT5_000421"
     CDS             42153..43403
                     /gene="clpX"
                     /locus_tag="ACHGT5_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN7216437.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            43486..44073
                     /gene="yihA"
                     /locus_tag="ACHGT5_000422"
     CDS             43486..44073
                     /gene="yihA"
                     /locus_tag="ACHGT5_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN7216438.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(44147..44302)
                     /locus_tag="ACHGT5_000423"
     CDS             complement(44147..44302)
                     /locus_tag="ACHGT5_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN7216439.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            44438..45766
                     /locus_tag="ACHGT5_000424"
     CDS             44438..45766
                     /locus_tag="ACHGT5_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216440.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            45777..45986
                     /locus_tag="ACHGT5_000425"
     CDS             45777..45986
                     /locus_tag="ACHGT5_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216441.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        15195 a         7907 c         9871 g        13072 t
ORIGIN      
        1 gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca aagccattca
       61 atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat actttgtcaa
      121 ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg tataaggtga
      181 tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc caaaatttat
      241 catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat tcccagttga
      301 ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct gcttcttgga
      361 tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa tgatttttag
      421 caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg ttccatttta
      481 cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct ttgatctttt
      541 gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc attctaaaac
      601 gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt ataaaaaacg
      661 aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc tcaaataata
      721 aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata
      781 tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag
      841 aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat
      901 aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc attagggaag
      961 atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca aacgggacaa
     1021 aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa
     1081 gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc
     1141 atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg
     1201 ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac
     1261 atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc accatcctca
     1321 ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa
     1381 tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca
     1441 gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc
     1501 tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct
     1561 ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca
     1621 ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag aggagagtac
     1681 ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt
     1741 ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc atcatcttct
     1801 gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg
     1861 aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg tactccttat
     1921 ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta
     1981 gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca
     2041 ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga ggatttgtta
     2101 gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact accgatcttg
     2161 tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg tgcctatgca
     2221 gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt
     2281 tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag tgaaagaagc
     2341 tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat tatctataac
     2401 tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag ctactacaga
     2461 tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag atgtttgatc
     2521 tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt catggttact
     2581 tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca catctttcgg
     2641 atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat aatgaataac
     2701 atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag ctagccaaac
     2761 acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg actgagaaga
     2821 ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg tactactaga
     2881 tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat tcgtttcagt
     2941 agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga ttgaactttt
     3001 gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc ctacaaggaa
     3061 taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct cattattttt
     3121 ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt ctatgaatat
     3181 aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa tcatcagtaa
     3241 aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt aggagaaagg
     3301 atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa tcgttttttc
     3361 tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga attctatagg
     3421 agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt ctataaaatc
     3481 ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc cggtgtgttt
     3541 gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa tagcagctac
     3601 tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat tttttatgtc
     3661 ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt gttcgattaa
     3721 gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga tagaagcagt
     3781 tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac cttcccaatc
     3841 ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct cctttactat
     3901 ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt agcgacagcg
     3961 aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa ttaaatacga
     4021 actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat tttgcataca
     4081 tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga aactggaagg
     4141 caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca tgaaaacaac
     4201 cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc aagaagtcga
     4261 ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta tatgagaggt
     4321 tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat agcttttcat
     4381 atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt ttatcgagag
     4441 gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga gctaggtttc
     4501 cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga ttttaagaag
     4561 gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat agaaacaata
     4621 aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga gcatatctgc
     4681 tattactgtg attttaataa agttttttta gaagggcagc cagtagatga gtatgtggaa
     4741 atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa tttagaaaca
     4801 ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga tcgactgtta
     4861 tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt agaagcgaat
     4921 ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt caatcgttta
     4981 tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg taaacacacc
     5041 gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac caatgtaagc
     5101 atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga tactttggaa
     5161 aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct cgaaaacaaa
     5221 acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca ggaaacggaa
     5281 accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca ccaatatgaa
     5341 ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta ctggaacaat
     5401 gcccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat tcgttaccgc
     5461 aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg cccggtattg
     5521 gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt aggactaaga
     5581 aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac aattgaatcg
     5641 gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa tgaagaagac
     5701 cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga gcgctttttg
     5761 tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa aaaagttcaa
     5821 aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa ttatttagaa
     5881 ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca ggagaagggc
     5941 ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa tgagaggtga
     6001 ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa cactatacga
     6061 gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag gccagctcag
     6121 caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg aagactcact
     6181 cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat catttgttaa
     6241 aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta ggcagagaat
     6301 ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag ctgacaagct
     6361 atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc tttcgtatcg
     6421 tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg aatgtagaaa
     6481 gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa atggtacaga
     6541 ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa cttcgtacgg
     6601 agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct cagttatttg
     6661 acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa atgaatctct
     6721 tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct tttatgaaag
     6781 actcagacgg acttacacaa atgatcactc cgatggatag caatattcat atcaagattg
     6841 gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca agttacgaga
     6901 tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg ccttattctc
     6961 ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt gctgattact
     7021 accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg cagctgatcg
     7081 ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag gaagactgta
     7141 tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag ccagaaccgg
     7201 aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg gaagaattcg
     7261 aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg cgggctcgtg
     7321 ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta caaaaatacc
     7381 gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa cgtgcattag
     7441 ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa atggtcttgg
     7501 aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct aaaggagaag
     7561 cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa gatacaccag
     7621 ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc gtgttaagac
     7681 caaccatggt cattgttgca caataattgt gcggatataa aacttacact ttcaattaaa
     7741 gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga ctaactctgc
     7801 agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag gaaaccgtac
     7861 aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag ttgcaaaacg
     7921 tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg gtgaagcagg
     7981 ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag caatgatcct
     8041 tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa aagcagttat
     8101 cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg ctggtaagat
     8161 cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc ttgcctatgg
     8221 tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg gcggtacttt
     8281 tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa cagctggcga
     8341 caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg tagcagaatt
     8401 caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac gcttgaaaga
     8461 cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa tcagcttgcc
     8521 atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga cacgtgcaaa
     8581 atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac gtcaagcgct
     8641 aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg gtggatctac
     8701 tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac caaacaaatc
     8761 agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg tgatcactgg
     8821 tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta tcgaaacaat
     8881 gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa gtaaatcaca
     8941 agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat tacaaggtga
     9001 acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag atattccagc
     9061 agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa acgggattgt
     9121 aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga tcaaatcttc
     9181 ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag caaatgcaga
     9241 agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg cattgctatt
     9301 ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag aagtgaaaaa
     9361 agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca tcgaagaaat
     9421 gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta aactatacga
     9481 acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg gagcagatga
     9541 tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag ttcactgaat
     9601 ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag tttgaagtat
     9661 cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat gatgagaacc
     9721 aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag ggctctcaaa
     9781 aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa aataccatcc
     9841 tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag aagcatatga
     9901 gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg caggcacaga
     9961 tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg gcttctctgg
    10021 tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag gcggtcggtc
    10081 agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg acttgtcttt
    10141 tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag aaatctgtca
    10201 tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc ataaatgtca
    10261 tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga tgagccgtca
    10321 aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc caacctgtca
    10381 tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag ctggtgtaga
    10441 agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg gcggaccatt
    10501 tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc gtgaaggttc
    10561 tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg atgaagtcca
    10621 agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac agacaggtac
    10681 gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa caggcgacca
    10741 acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa gagaagcatt
    10801 acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag acggtttctt
    10861 cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct agtaaaaacg
    10921 ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg tttttttgta
    10981 gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg tcatagtttc
    11041 taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag gtgtttttat
    11101 gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg aagcatgcca
    11161 aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata cgttttacat
    11221 ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct aaatcttcaa
    11281 aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact tgtttggcat
    11341 gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt ccaaagttta
    11401 gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat gatagcaatg
    11461 aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca gtgagcaaag
    11521 cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg atccgttctt
    11581 gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga gaagaaaaat
    11641 caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa tgaaccgatg
    11701 gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg aaaacaagcg
    11761 atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt cccttcatgg
    11821 cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa atcttttgtc
    11881 cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa aaagtttccg
    11941 tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc gtgcgtaacg
    12001 ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac atcaccgacc
    12061 atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg gttcatcgct
    12121 agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc aggatacata
    12181 tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt ctctgcttct
    12241 tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa aatagaaaga
    12301 tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac caagttgata
    12361 tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc agaaggttct
    12421 tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg gccaatgata
    12481 cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt gttatcgccg
    12541 tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca ccttcttttc
    12601 taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga tcgcaatgat
    12661 caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag tttgcagcaa
    12721 ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa cgaactgatt
    12781 gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt gcattgtccg
    12841 attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa cggcgttgat
    12901 cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg tgataacacc
    12961 agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga aaataaagaa
    13021 cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag caattgtgcg
    13081 aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta ctatagccaa
    13141 agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca atgcttgata
    13201 gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact cactggaagt
    13261 ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg ttctttttaa
    13321 ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt ttttaacggc
    13381 aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt gtccttgttt
    13441 gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc caacttttaa
    13501 cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat attcagattc
    13561 atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg tttttccttt
    13621 caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt ggatcccgct
    13681 atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta cagtcattcc
    13741 ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag agaaaccacg
    13801 gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca attttacatc
    13861 gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag cagagtcact
    13921 ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc ttgtgcttga
    13981 ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg tcacaagtcc
    14041 tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca acctcctaaa
    14101 tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact gtaagctcga
    14161 aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac atgtataata
    14221 agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa ggaatgcctt
    14281 tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa aggaggacga
    14341 agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata ccaaccggct
    14401 ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg gaaaaaagca
    14461 caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa tcttattgag
    14521 aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg tcagctgtac
    14581 ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc ttattatgat
    14641 tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa agattcaagt
    14701 gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt agaaagaaat
    14761 gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc ttttgagtac
    14821 caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca gttgattcgt
    14881 gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg tcgttttcga
    14941 gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc cttacgtgtg
    15001 gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac aggtgaagtt
    15061 ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac gaatgatgaa
    15121 catatggagc atgccgtagc gaatatcaaa gcggaattag aacagcgttt aactgtcttg
    15181 cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa ttacgatatt
    15241 gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg gcatatggac
    15301 ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga agattttttg
    15361 atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta taatggtgac
    15421 cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc tttagacaat
    15481 cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta tgtctctgcg
    15541 acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat catccgtccg
    15601 acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat cgatgatcta
    15661 gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac gacattgacg
    15721 aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa agtcaaatat
    15781 ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct tcgattaggt
    15841 gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt acctgaagtt
    15901 tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga acgttcctta
    15961 gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat gtacgcagac
    16021 aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg tacgattcag
    16081 cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga aatccgagat
    16141 ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca agtctcgtat
    16201 gaagaaatga cgaaagaaga aaaagatacg ttattgatga aattagaaaa agaaatgaaa
    16261 aatgcggcga aagcattgga ttttgaaaca gccgctaatg tgcgagatat gattttagaa
    16321 ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat tcacggtgca
    16381 cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt cgtggtcgtt
    16441 acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta cgctgaaggg
    16501 cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg acaaatggat
    16561 aagccggacg tagatagcat cgacggattg agtccggcga tttctattga tcagaaaaca
    16621 acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga ctatttacgc
    16681 ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga gattaccagt
    16741 caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac aaagatccaa
    16801 gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt cgaacgaatc
    16861 caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt aagtgaagtg
    16921 cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg gatcattgtg
    16981 aaagaaggga ttcgttctcg tctatttgat tctttagagg cggctctgcg attggcagac
    17041 ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga acactatgcc
    17101 tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc attcaatgcg
    17161 ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt cgatcaagat
    17221 ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc atggaatcca
    17281 atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt cggtatcgat
    17341 ttagatacac cattcgaaga attaccggaa gagcatcagg atatcatctt gaatgggaca
    17401 gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga tgtagaaacg
    17461 actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa cagtgatttc
    17521 acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg taaaggttat
    17581 cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg gcaaacaaat
    17641 gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc tgaacaagag
    17701 aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt cttggaaaat
    17761 gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg gggagaagcg
    17821 cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt gtatatttta
    17881 gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg ctcattaaag
    17941 aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga tacgatgtgg
    18001 gcagcagatt atctgattga cgtcggacct ggggccggag aacaaggcgg acaaatcgtg
    18061 gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg gaaatatctt
    18121 tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg gaaagcaatc
    18181 aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt tccattagga
    18241 gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt caatagtatc
    18301 ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg taaattcaag
    18361 acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag cccaatcggc
    18421 cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat ccgtgggtta
    18481 tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt tagtttcaac
    18541 gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat cgaaatgcac
    18601 tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gcaaacgata taactctgaa
    18661 acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat gacagtagaa
    18721 gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca aacgattgtt
    18781 gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc cggtggtgag
    18841 gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa gaatttctat
    18901 atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt gttgcatgta
    18961 ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa tctagatgta
    19021 atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg tggaggaacg
    19081 atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta tacaggtcac
    19141 tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa aaaaacataa
    19201 aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta catggtagtt
    19261 ctttctacac tgattggtat agacaaaaac ttcctataag ttcaatccgt tcaaaaaaga
    19321 attgagcaat cggaggagtt tcctttgatt gcttttttct ttatggtatg atattccttg
    19381 tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga tgaacagtat
    19441 tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt atggtatggc
    19501 acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg atcagttagc
    19561 ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg ataaagaccc
    19621 tgtgtttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata agagtaaaaa
    19681 atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac ataaacaaga
    19741 gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa gactgatcaa
    19801 taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta aaacattgat
    19861 cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt ttgaattccc
    19921 agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg aaggaagaaa
    19981 aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc ggatatacga
    20041 agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg atagtaatat
    20101 gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt gtcttggtca
    20161 tccactgtta gaagatccta caccagcatc gattggtaat tacgttgtga tcatgccaga
    20221 ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg aatatacagg
    20281 ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat tatttgaaat
    20341 caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga atctggctcg
    20401 tttcgtcaca gaagatcgag tgtttaacaa accctttgtt gaaacgacct atggaacgaa
    20461 tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt tagaatatgc
    20521 tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc gttacggtac
    20581 tactgtgttt tatgaaaaag accggtcaat caagcgctgg ttgttgatga aatatatgtt
    20641 ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct agttcatgga
    20701 gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt ttgttcgctt
    20761 gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg tcttattcaa
    20821 tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag aaaatccaat
    20881 gccgtttctg ctggatgata ttgagaaaca aaatttattg cagccgaact tcattgtgtt
    20941 gacttgtaac accgcacact acttttttga agaattacaa gcggcaacgg atattcctat
    21001 ccttcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga cagggagagt
    21061 agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg aagtcaaaaa
    21121 tcttggattc gaaacagtga ttcctgatac tgttcttcaa gaaaaaatca attatctgat
    21181 ctatcatgag atcaaggaat cggattattt gaatcaagag ctatattacg aaatcttaga
    21241 agaagcggta gagcgcctaa actgtgaaaa agttatctta ggttgcacag aattgtcatt
    21301 gatgcatgaa tttgctgaag ataaccatta tccagtgatc gatgcccaat ctattttagc
    21361 agatcggacg attgaacgag cgctagctga aagaagcaag gcattggata cagcatcgga
    21421 aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc aaatcttttt
    21481 gtaaaagaaa aagaaaactg gtaatgatac gcccggaatg atataatttt actatgcaca
    21541 atgtgagagg agttcgtaat tatgacagat aatttgcaat tagtcatcat aacaggtatg
    21601 agcggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta tttttgtatc
    21661 gataatatgc cgcctcgatt gattccaaaa ttctgggaat taatcaaaga atcaggcaaa
    21721 gtcacaaaaa tggctttagg agtggattta cgttctcgtt ccttctttga agaaatccaa
    21781 aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt atttttagat
    21841 gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca tccaatggcg
    21901 atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga cgacttgaaa
    21961 gcccaagctt ccgtgatcat tgatacgacg acattgacac cacgacaatt acgtgaaaaa
    22021 atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt tgtatcattt
    22081 ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg ttttttgccc
    22141 aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc agtctatgat
    22201 tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga tcttctggaa
    22261 actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc aatcggctgt
    22321 acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc tttagggaaa
    22381 aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac ggtgaaccgg
    22441 tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc ggcggtggca
    22501 ctggtctgcc agttatcctg aaaagtttgc gaaatcaagg agcagatatc acagcagtag
    22561 tgactgttgc agatgatggg ggaagcagcg gtgcaatcag agaatcgatc gctatggctc
    22621 ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa ttctatgaag
    22681 atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat acgatcggta
    22741 atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg attcaattat
    22801 tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact cctttgacct
    22861 tgcatgctgt ctttaaagac ggaacgactg cagtaggaga atctaaaatc gcgatcgacc
    22921 gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa gccaaagcgg
    22981 ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga cccggaagtt
    23041 tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca atgctcaaga
    23101 caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa acggaacatt
    23161 tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct tttatcgata
    23221 cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag atctatgatg
    23281 agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga tgtcgagtga
    23341 tttccaccga ctttttggaa ttgaaagatg gtggtgtctt ccacgatgga gacaaagtag
    23401 tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat tgttggagaa
    23461 aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact ggtttggaag
    23521 ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac ggctctttag
    23581 gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca attgccagac
    23641 gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc gttcgtaaaa
    23701 aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc acaaaagatg
    23761 tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt tcggacgaga
    23821 ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg gcctcaggtt
    23881 cagtcaataa tccggaaacg agccgttatc atttggaaat attttctatt tatgaacagc
    23941 ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga acgcttgaac
    24001 ggcgcaatgg ctatatttct tatttaaaag gagcagaaaa aattgctgat ttcttaacat
    24061 tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta cgggatatga
    24121 gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag acgattgatg
    24181 cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt ctacatgccc
    24241 ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag gtcagcttga
    24301 aagaattagg ggaaatgatt ccctcaggtg cgatatcaaa atcgggaatt aatcaccgaa
    24361 ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa tccaaagaaa
    24421 aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag ttatacttgc
    24481 ttttcttccg tcaaactata attccatctt tactggagta acataatttg gaaaggcttc
    24541 tattgttttt aatacaatcg tataaaaagc ggggagacaa ctgaatgaag attttagttt
    24601 tgtacataaa tttttgctta atatagaagt cctgatatag ataagatcaa gtatttatct
    24661 ataactggcc tgattaatta aaaaactgga aggaactgct cgatcatcct tgagaactgc
    24721 tcttcttact tctggtcaag attctcgtgg ttggtggata aggatggttg ataagttcat
    24781 tggattagct agtcgaaaac aaccaggtgt aattaatgta gaagttttaa caaggggatg
    24841 gagtgattta ttcagtgata atcataagtt cataaatagt aattgagatg tatggttgga
    24901 atacgttata cctgaacttg aactaccaag atttggttta gaatttgaga atcgatgttt
    24961 ataaagggag ggcaaaaatg cgttttttta caacttttac tatcattatg attgcagtac
    25021 tttttatttt tctagatata gcaaagcgaa atacagcatt tttattatat cgggttttac
    25081 ttcgagcagg tctcataaca tttatatcga tcgttggatt ttttctattc acagttattg
    25141 tatttatttg gagaactcca gcaccaccat tgccagagat tacatatgga gaatttcctt
    25201 ttagacttga gtatgaattg aatgaagaat tacatgtgat agaagacacg ttaatagttg
    25261 aatttgatgg atttggaatg aacgaaggca tagggagata tcgtagatgg acaagtcgtt
    25321 tagccagcgg agaagattta gtgttgctgt tagaagttag tgataataag caaatatttt
    25381 attttccagg acctgctaat tattacatgg gagataggtt gaatggttat aatcatactt
    25441 ttcctagtgc ttctttcatt gaaagggaaa gaggcattac tagaagggat attttacatg
    25501 acaaagaatt attagaacaa tttggtccgc ttgatcaaaa tacaataaat gaagaagaat
    25561 tgttaaatca atataatata aggctagtga attgggaaat atctgaaccg attgttaaca
    25621 attttggtga ttagaaaaca attacgataa atagaaacaa gccaaatcat cagcaagtaa
    25681 aagctgatga tttggccttt cttatacgag tagtaggaat tcatagcgaa tattattacg
    25741 atcagtagat attttgatat taaaggagta ttacgaacta gtatgtgaga atgtagaggt
    25801 gatttgcgaa taatgagaaa tttatcaaat agaaacaaaa tattaatcat tattgttgtt
    25861 atagctgtgt ttcatctggg aacgaatgcg gttttaagta gaataatatt gggtcctaag
    25921 ccaccaagac ctgcgattac ccgaggggag tttgatttta gattagaata tgaagtagat
    25981 ggggaaagga ttgtgataga agatacaatt gttgcattat ttgatggatt ttctgcagat
    26041 gcaggtagca tggcatggta tcgcacgtgg cgtttgcatt tggcgagtga caagagggca
    26101 caacaattat tgttacatga gttagatgat ggtcgaaaaa tatattatca ttttggagga
    26161 gctggatttt atatggggaa gttagtagca gtgtaactgt aacaaggaaa gatttgccag
    26221 gtcttggcat tcgaaactcc atattttata tcgagcgtgg gagagataca tctattggga
    26281 acaggaaagg gttaactctt gaagagttat ataatgactt tggaatccgt cttgttagtt
    26341 gggaacacaa tccaccaata gaaaatcgct ttgaataatc gaaatctatt aatccatttc
    26401 atcagccagt gaatgttgta atggaacaat ttggtttagt gttcaaaacc attgaaatac
    26461 taggctgtga tcagcaaaaa tgtatctgaa taaaaaagag agcaactatt aaatgagagg
    26521 agttaggagt tgttagcttt atacgaatca aagtggcaaa tacagatgcg tatgcatttg
    26581 tgtttgccat cttgcgtttg gtagacgaga taggtataag ctaaagaaac aacttgttta
    26641 aaataaacga cctagctcaa caaacgacca tctaatcaac taacagcata cataaaagca
    26701 actatttttg aagtagaatt gaaatcggga taacttaaaa agtgattggg atactagtgt
    26761 gaatcaccgt acataaatat tactcgttaa gggaatttga gaaattttgt atgaaaattt
    26821 aagaattagt tagtatgcgc aagtcaaaaa tatgaataaa ttatttggtg gagataattt
    26881 gctcagcttg tcataagaaa tgagataatc tggcagatgc aagcttttgg acaccattta
    26941 ttcggaataa ggaggctggg acagaagcgt ttaactccaa gaaataagaa ggagttgctt
    27001 ctgcttccgc cgtttatagg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc
    27061 tacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
    27121 attcatcaaa gatttcttta cccaagatga cagtcggaac aaatcgaata tgtgcttgct
    27181 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
    27241 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
    27301 cgaatatttg tttgatctct ttgatcgctt tttgaccatc gctgattgtg agataccggt
    27361 gcatcacgtt tcctcgctgc aagctttctt tttctttatc gaacaatttg atcactcgct
    27421 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
    27481 ggcaatacgg acaagatagg ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
    27541 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
    27601 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
    27661 ctgtttgtct aaaaattaga tgaccaattc aaatagacat atgtgaaata tttttataaa
    27721 taaaagaaaa aatggaatgt tttacgttat aatggaacag ataataagag agaatatgaa
    27781 caaggatgat gaatcatgcg aaaaatgaaa acaatggatg gcaatacggc agccgcatat
    27841 atttcttatg catttaccga gattgctgca atatatccaa taacacccag ttcgaccatg
    27901 gctgaattag tagatcaatg gtcagcagca ggaaaagaaa atgtattcgg tcaaccagta
    27961 aaagtagtag aaatgcaatc cgaagcagga gcagctggtg tggtccatgg atcattgaaa
    28021 actggtgcac tgacaactac atataccgct tctcaagggc tgcttttgat gataccgaat
    28081 atgtacaaga ttgctgggga actactgcca agtgttttcc atgttgctag tcgggcagtg
    28141 acgacaaatg cgttgaatat tttcggagat cacggagacg tgatggctgc aagacaaaca
    28201 ggatttgcta tgttagctga aagcagtgta caagaagtga tggatttagc acctgttgcc
    28261 catcttgctt ctattgaagg agaagtacct tttttgaatt tctttgatgg attccgtacg
    28321 agtcacgaga ttcaaaaaat cgaagttttg gaatatgaag aattagctcc tctgatcaat
    28381 caggagaaat tagcttcatt tcgtaaacga gggatgaatc ccaatcatcc gactgttagt
    28441 ggtacgaacc agaatccaga catccatttt caacaacggg aaacgatcaa cccctattat
    28501 gataaattac ctgaaatcgt tcaaaaatat atgaaagaga tcaaccgatt acgaggaact
    28561 tcttatgact tggtgactta ttacggagca gacgatgcgg aagaagtcat tgtttctatg
    28621 ggatcagttg cacagacgat tgaacaaacg attgattact tgaatgcaaa cggacggaaa
    28681 acaggttttt tgaatatcca tctttatcgt ccttttccag tggaaacatt cgtgcaaaag
    28741 ttgcctcata cagtaaaaac aattgctgtt cttgatcgaa gtaaagaacc aggggcaggt
    28801 ggagaacctc tcttattaga cgttcaaagt gcgttatatg attctgaact tcgcccggct
    28861 gttattggtg gtcgctacgg attggggtct aaagatgtga ctcccgacca aatctcagca
    28921 gtttttgatg aattgaaaaa agacccaagc atacgcaaaa aacggtttac catagggatc
    28981 gtggatgatg tgacttatca atctttggaa ccaaaggaaa gtctggattt gacagaaccg
    29041 caaacattcc aggccaagtt ttggggattt ggatctgacg gaacagttgg agcaaataaa
    29101 tcagctatca agattattgg agatcacacc gataaatatg cgcaaggcta tttttattat
    29161 gattcgaaaa aatctggcgg tctgactgtc tctcatcttc gttttggaga tacgccgata
    29221 cgttcagcgt atcttgtcga gcatgcggac cttgttgcct gtcacacacc agcttacctg
    29281 catagctatg acttggtcaa aggattgaag ccaggtggca tttttttatt gaatactctt
    29341 tggagcgacg aacaattaga aacgcatctg cccttaaaat taaaacgtta cttggctgaa
    29401 aataacatta gattctatac gatcaatgcc atgagattgg cccaagaagt cggtttggga
    29461 cgccggatca atacggcaat ggaaacggct ttttttaaat tagctgacat cattcctttt
    29521 gatgaggtgc ttccgctgct aaaagaagaa gccctgaaaa gctatggaca taaatcaatg
    29581 gctatcgtag aaaaaaacat acaagcaatc gatcggacga ccgaactgct tcatcaagtc
    29641 gcgatcccag accattggag aacgttggag atgcctgaaa aaatcaataa aaggaacact
    29701 ggttatatac aagaaatact tgaaccagtg aatgcacagg aaggaaatca actctctgtt
    29761 ggtacacttg tagcaaatgg aatgacaact ggcgaaatgc ctttaggaac ggcttctatg
    29821 gaaaagcggg gcgtcgcttt agaagtacct gagtggatta gtgatcgctg tacgatgtgt
    29881 aatgaatgtg cctttgtttg tcctcatgct gccatccgtc catttttagc agatgaagag
    29941 gaaatggaag aagcgccaga aggatttatc gtgagagaaa tgcgaggcgc agacggcgta
    30001 aaataccgta tccaagtttc agtggaagac tgtacaggtt gtggtctgtg tgtagaggct
    30061 tgtcccgcaa aagaaaaagc tttagtgatg aagccttatg aagaacagaa agaacaagct
    30121 gtgaattggg cttttgcaat gacattaaga caaaaagcaa atccagtcaa aggaaagaat
    30181 actgtcttgt ctacacaatt caatcaaccg cttcttgaat tctcgggcgc ttgctcaggc
    30241 tgtggcgaaa caccttatgt aaaattattg acacaaatgt tcggtgatcg aatgatgatc
    30301 gcgaatgcga caggctgttc ttctatttgg ggcggtgctt ctcctgcttc tccttatacg
    30361 accaatgaat gtggtcaagg gcctgcatgg tcaaattctt tattggaaga taatgctgaa
    30421 tttggttatg gtatgcttct agcggcacag actcgtcgcg aggctttggc aagttcaatc
    30481 aaagaagtat tgccaaaagt atcagaagaa ctacgagaat tgatggaaga ctggttggta
    30541 cacatgtttg agagcgaagg aacgcagcaa agagcagcaa aactgcaagc tgcgatcgaa
    30601 agcgaacgat caattgtccc tgaactggag gatatataca agagaaagga tctattcgta
    30661 aaaaacagcc aatggatgat tggtggagac ggatgggctt acgacattgg ttttggcggg
    30721 atcgatcatg tacttgccag cggagcagac gtgaatattc ttgttttgga taacgaagtc
    30781 tattccaata ctggcggtca aacctcaaaa gcaacaccag cttcggcaat cgctaaattt
    30841 tcggctagtg gaaagtatgc ttcaaagaaa gatctaggaa tgatggcgat gacatatgga
    30901 aatgtttatg tggcgcaaat tgcttcaggt gccaatcaaa tgcagacgat aaaggctttt
    30961 gaagaagcag aaaagtatcc tggaccttct ttaatcattg cctatacgcc ttgtatcaca
    31021 catggtttgc ttggtggaat gaaagaatcg cttaaagaag caaaagaagc tgtttcttct
    31081 ggctattggt cattatatcg atataaccct gcaatggtcg aaaaaaacaa gaacccaatg
    31141 actttggact acaaaaaacc tgatttctct gctatgcctg atttcatgag gaaacaagtt
    31201 cgcttttcct cgctagagaa cgcaaatcca aatcttgcaa aagctttatt tgaaaagact
    31261 atccaagacg ctaaaacacg tttttacaac tatgctgcac tgacgggaga attagaaaaa
    31321 atcaagaaac gacttgaacc agaggaatct atcgatgtac ccgttaagac tagaaaagaa
    31381 aaaacgacgg accttgaagc agaagaaaga agagctgcca gagcagaacg acgtaaaaaa
    31441 atggaacaag ctacaaaaga aaaaaaggaa aaataacaga aaaaatataa tgaataattc
    31501 tctgaagttt aaaaatattt attgcagaga aaaataaaac gcatacttgc aattttcctg
    31561 aaaattatgc gtttttctca agaaaaatac gagcagatca gcctctaaaa ttaaaacata
    31621 tgatatacta gagaggaaat ctcaaaagag aagagaggct ttttatgtca tttcaaatcg
    31681 gggaactttt taatttggac tattggcagc aaatgttttc cggtgacttg ttctctctga
    31741 attttttggt gaacatactt gatattctgg ttgtctggta tcttgtatac aaactgatcc
    31801 aactgctgcg aggcacaaaa gcgattcagc ttctgaaagg tgtagctatt tttattgtta
    31861 tccgaatcgt agcggaaatc attggtctgc acaccttgtc ttggctgatg aaccaagtca
    31921 ttacgtacgg ggtgatagct gctatcgtga tttttcagcc ggaaatccgg agaggactgg
    31981 aacatcttgg acgcagttcg cttttcaaac aatcaaaaag cgaacagcag gaagatgaga
    32041 agatgatctt gtcttttgac aaagccatcc aatatatgtc caagcgaaaa atcggtgcgc
    32101 taattactat cgaacggcat actggattag atgagtatat tgagacagga attgcattag
    32161 atgcagatat cactggagag ctgctgatca atatctttat tcctaataca ccgctgcatg
    32221 acggtgcagt tattgtaaaa gaaggaaaga tcgcagtagc aagcgcctat cttcctctat
    32281 cagaaagtat gttgattcca aaagaattcg ggactcgaca ccgggcagct gtcggtataa
    32341 gcgaagtcag cgacgcgatt accatcgttg tctctgaaga aactggggat gtcagcatta
    32401 cattagataa tgaactgatg gctggcttat cccagcaaga atatttggca attttgcgta
    32461 gagaactgat tgctgaagag aaaacaaaaa acaagaaaaa cgtgattcaa tctttcctag
    32521 acggtatgac taagggaggg gatcgcaaat gatctcaaaa gagaaacgga caagcattct
    32581 ttacagtttt attgcattgc tgttcagtat tgcgctgtac ttcaatgcaa atggccagtc
    32641 cgttccaaat acgctttctg gaaatgaagc gtataatcaa acagttactg gggtgccaat
    32701 caagatttct tatgattcaa agcgctacta tatccatgga tatgaaaatg cagtgactgt
    32761 caaactttcc agtgcaaacc gagttcagtt gaatgcagag gcaaacgaag atacacggat
    32821 gttccgcgtc acagcagata tcactaagct gggcgaagga acacatgagg ttccgctaaa
    32881 aattcaaaat ttgagcagtg ctgtcacagc aaagatcgaa ccatcgacta ttacagtaac
    32941 ggttgagaaa aaggtaataa aagatttcga tgtggaaacg ttgattcctt caacgttcac
    33001 accatctgga tatgaacttg atgatacttc cgttagtcct aaaaaagtct ctatcactac
    33061 aggtgacaaa acattggcag aaatcaaacg agtagtagct gatgtggacg cttcgatggt
    33121 cacagatgat gggattaata gtgaggtccc aatacaggcg ctgaatgcag caggggaagt
    33181 attaagtatc gtctctgatc cagtgcaagt aacagtgaga gctgattcga tcaagccaag
    33241 caagtcggta cgactttatg ggatacagca aggaacacca gctgccgggg tagaaagcta
    33301 tgatttcagt ttttcaaaac tggaagcaga agtaagcggc tctagcgatt tgcttgcatc
    33361 gatcggtgac agtattgctg taccgatcaa tgtctcaggt atcaatcatc gaacaactcg
    33421 taaaattgaa ataccagtag aagaggggtt aagtatcaag cccgaatcag ttagtgtgga
    33481 gattacaccc gtgttgcaaa cgtcaagcag tagctctgct tcgacgagca gtcggacaca
    33541 agaaaataca agtacgagta gaagtatttc atctgctgat tcaggaacgg aaccatcgac
    33601 aacaaccatt tcttccagtt cggaatctgg gacaagcagc tcgaccacaa gtacagaaga
    33661 aacatcagaa acaagcgaat caacacaaat gagcagaaca aattcctcag aaaattaagt
    33721 taaaggagta attttacaac tatgggtaaa tattttggaa cagacggcgt acgcggtgaa
    33781 gccaacaagg aacttacgcc agaacttgca tttaagctgg gacggtgcgg aggttatgtc
    33841 ctaagccagc atgaagaaga aggaaaacgc ccacgcgtgt tagtagggcg ggatacaaga
    33901 atctcaggtc aaatgttaga agaagcgctg atttcaggac ttttatcggt gggtatcgaa
    33961 gtgttccagc taggtgtgat ctctacacca ggtgttgctt atctgacacg tttgcaaaaa
    34021 gcaagcgcag gggtcatgat ttctgcttct cataatccag cacaagataa tggaatcaaa
    34081 tttttcggtg cagatggttt taaactagta gatgaccaag agctagaaat tgaagcatta
    34141 ttagatgctg aagtggatga attgcctcgt ccatcagctg aaggattggg aactgtagaa
    34201 gaatttccag aaggcttatt gaaatattct caattccttg tacaaacgat caacggtgac
    34261 ctttccggtt tgaccatttg tgtcgatgca gcaaacggag caacttcaac aagtgttaat
    34321 cgtttgtttg ccgatcttga aacagatttt tataccatgg gcacaaatcc agacgggtta
    34381 aatatcaatg atggcgtagg ctctacccat ccagaacgtt tagctgagat ggtcgttgaa
    34441 aaaggagcag atgctggact agctttcgat ggcgatggcg atcgtattat tgcggtggat
    34501 gaacttggaa acattgtcga cggtgataaa atcatgttta tctgtgctaa atacttggca
    34561 gaaaaaaatc gattgaaaaa agatacgatc gttacgactg tcatgagtaa cttaggattc
    34621 cacaaagcgg tggaaggaat cggtttgaaa gatgtcgtta cacaagttgg tgatcgttac
    34681 gttgtagaag aaatgcgcaa aaacgattat aactttggcg gcgagcagtc tgggcacatg
    34741 attttcttag attttaacac aactggagat gggatgcttt caggaattca gttactgaat
    34801 atcatgaaac agacaggtaa gaaattatca gagcttgcag cagaggtaac aatctatccg
    34861 caaaaattag tgaatatccg agtaagtgat aaaaacggtg cgatggatgt tccggcaatc
    34921 aaaaaagtga ttgaagaggc agaagctgaa atgggatcag aaggaagaat cctagttcgt
    34981 ccttccggta cagaaccatt acttcgagtg atggcagaag cgcctactga tgagaaagtt
    35041 aattactatg tcgataaaat cgcggcagta gtcaaagaag aaatcggact ggattaagag
    35101 atattttcat aaaaaataaa agaacaagga ccagtgtcag atgtctgaca ctggtccttg
    35161 ttcttttatt attctactgt cactgatttc gctaaattac gtggtttgtc aacgtcgaat
    35221 ccccgtaata gcgttgcgta atatgcaagt aattgagtcg gtacgactgt ggttagagta
    35281 gttagataag ggtggacagt tggtagcacc agctggtcac tttccttttc taatccctct
    35341 gagacaataa ctagtgtttt agcgccacgg ctttcgactt ctttcaggtt tcctcgagta
    35401 tgcgcaccag tcacttcttc actgatgatt ccaatcactg gtgttccttc ttcgatcaaa
    35461 gcaatcgttc catgtttcaa ttcgccagcg gcaaaacctt ctgcctggat ataactgatt
    35521 tctttcaatt tcaaagcagc ttcaagagaa acgttgtagt cagcgccacg tccaatgtaa
    35581 aaggcatttc ttgtatcgct gagatatact tctgccaact cttctaaata atctttttgg
    35641 gcgatgaccg attccatcgc attagcaact aaactcaatt catgagtgat atcgaataca
    35701 agagaagttt caaattcttt tttatcaccg atagctttgg ctaataaagt tagtacagct
    35761 atttgtgcag tataagcttt cgttgatgct actgcgattt cgggtccagc atgcaatagc
    35821 agtgtaaagg atgcttcccg cgaaagagta gaaccagcaa catttgtaat cgtaagtgaa
    35881 gggtagccga gacggttgat ttttaccaga acttggcggc tatctgctgt ttcgccactt
    35941 tgactcaaga aaataaagaa tggttttttt gaaagaagag gtgtgttata gccaaattca
    36001 ctggctaaat gaacctcaac agggatctgt gtcaattttt ccagagtctg tttacctgct
    36061 aaccccgcat ggtaactagt tccgcaagca acgatataga ttcgatcgct atcagacaat
    36121 gtatcgacca gcttttgatc aagagagaca cgattgtctt ctgttacgta tttttgagcg
    36181 atacgacgca tgataatagg ctgttcgtcg atttctttca gcatataaaa aggataggta
    36241 cctttctcga tatctgaaga atctacttgt gcttcgaatg aagaacgttc tacgatgtca
    36301 cctgcaaaat tttgaataat aactttttct gatgtgacgg taaccatctc ttcatcttca
    36361 atctcaacga attccttcgt atgagcaagc atagccattg catcgctggc aatcacattg
    36421 aatccatcgc cgagtccgat taatagtgga cttttatttt tagcgacgta aataacatca
    36481 ggtgctgtcc gatcaatcaa agcgaaagcg taagaacctt tgatgacccg caaagctttt
    36541 aaaaacgcct cttgcgttgt agtatccttc gcgaaagttt caattagatg agcgataatc
    36601 tcagtatcgg tctcaccaat aaagtggtca tttgctaaat aagcaatttt aagttcttca
    36661 aagttttcga ttacgccatt gtgaacgagc acgaagcgtc cactttggga agtatgtgga
    36721 tgggcgtttt cttcagaagg ttgcccgtga gttgcccagc gtgtatgacc aatacctata
    36781 tttcctgtag tatcaatatt tattttgttt ttcaaatttt gaattctgcc ttgtgctttg
    36841 accagataat cagttgtgcc atctgagaca aagataccag ctgaatcata accacggtat
    36901 tctaattttt ctagaccgtc aatcaatcct ggtgttacat tttttaatcc aatcattcca
    36961 acaattccgc acataaaaaa catcctactt tctataatga attcgtatga atgcttccag
    37021 ctgcaggaaa atccttttgt tttatctttg cagatatttt ttgtggattt tctgtagagt
    37081 gcagtcccta gggccatccg ccgaatcttt cgataaaccc tctcctcgtc atctgtttac
    37141 acagacctgg cgctatccta aaaggtataa ctatcctcct cttttgtttt ttatctggaa
    37201 acatttaaaa tcataacctt ttgcgaatgt tcttgtcaac aaaaaaagaa taaaaaataa
    37261 attggtatat atcaaatgct ttgtcaaatg ctctttttat gtcaaaaaat aatgggtttt
    37321 tatctatcat ctttttcagg agttcggtac aatagaaagt aatcggatag aggaggagta
    37381 aaaatggatt caaaaattga gatcatgaca ctgggaatgt taaaaaaaca attgtccaaa
    37441 ttcgaagcat cagctggtgt atcagatgat acgaaaatat ttttagatac tggctgggat
    37501 agcatccaag aaatcgcacc tgatgccttg gaagttgctg aagctcgtga gtttacggta
    37561 gaagatgaat tgacaaaaga gtcgttttcc gggtatgcgc gggaagaaaa agcagagcgg
    37621 ttcgacactt ctgagcagtc agaaactgtg atcgtcataa aaaacttgta ttagaaagga
    37681 gaaagaatga ataagagacg ttgggcagca gttctgattg ctgcaggatt atttgtggtt
    37741 tcgttagttt cagctagttt gactaagcct caggaaaacg atcaaattga gggattgaac
    37801 agttggttat atggggatga agaactagct tcaaatgtgt tggaagaggg agacagtacg
    37861 caaaaaatcg taaaattatc agtagatgga acgattgctg cgggaggggc ttcaggttta
    37921 tttgcttctg aaggttacaa tcataaaaat tttatggaac aactgaaggc gattgaagaa
    37981 gatccaacag tcaaaggtat ctttttagaa gtaaattctc ctggtggcgg tgtctacgaa
    38041 agtgcggaaa tcgctaagaa attggatacg attcgtaaag aacatgatat cccaatgtat
    38101 gtcagcatga aaaacatggc agctagcggt ggctactata tttctgctca agcagataaa
    38161 atctttgcaa cagaagaaac agtgaccggc tcaattggtg tgatcatgtc aggattgaat
    38221 tattcaggac ttctagaaaa actgggtgtg gaagatacaa cagttaaaag cggagcgttg
    38281 aaagatatgg gttcaggaac gcgaccagag acaaaagaag aagaagcggt gcttcaagca
    38341 tatatcgaca gcgcatatag tcgttttgta gcaatcgtca gcgagggacg gaataaatct
    38401 gaagaagaag tcaaaaaaat tgctgatgga aggatttatg atggtgtcca agcaaaagaa
    38461 gtaggtttag tcgatgaatt gggatttcct gaagatgctt taaaagctat gcgaaaggaa
    38521 caacagcttg aagatgcgga actagtagaa tattcttcca cgccatctgg gtttgctagt
    38581 acatggttag gaagtaaact agcagaattc cagggattga agccttctga gactagtcag
    38641 atcttatccg ttcttgagaa tctagggaca gcggaatcac caaaagcaat gtattattac
    38701 ggaggtgagt aagcatgtca gagctttcaa atgaaaataa ggaacaagaa aaagataagc
    38761 aacagcagcc tgctgtagga cctgactcca acttttccca aaaggtactg agctcttttg
    38821 aaagagagcc tttgacgccc gaagaaatca aaaagaaaca acgtaaatgg aaaaagtata
    38881 cggacacaag aaatgaaccg ccaaaagcaa atgattttcc taagtatttc tatgcaggtt
    38941 tttttatccg aatgtttgct ttcttggtcg atcttttgtg tatcggagct attacgcgtt
    39001 taacacttgg tattgccaaa aatctaggtt ggcttaattt ttcagattct tatctgagta
    39061 tctacggatt cacagcctta cttatttatc tagcgtattt tattttattg acgaaattca
    39121 atcatggtca aacgattggg aaaatgatct ttggtattcg agtcatcagc tttgatgaag
    39181 aagaacttag ctggtcaacc gttttagtta gagaaggtgc ttgccggttc attctaaaat
    39241 ttcctttgct gatgttaggg tatcttccag caatctttaa tcagaaaaag caacatctag
    39301 gcgatttttt cactaataca agtgttgtta cgatcaatct gatcaaagca ttcaaccagg
    39361 aagtcaacag ctgatgagca ataaaaagca gtaacagttg aaagcacctt ctaaaagtca
    39421 gatctaaaat ctagctttta gagggtgctt cagttatttt taattgtcat gctttttttg
    39481 ttttgctact attgaacatg agctgaacat gagcaagagg aggacactgc gtgaagccga
    39541 ttgattttcc agaaaaatat gagaatttaa tgcgagtagc acaacaagca ctagcaaacc
    39601 agcaatatca gcaggcaaaa gaattatttc aacgagctta cgagttgaaa gaaagctttg
    39661 aggctaatag cctgcttgtt ttttgtcttt acgagttgga cgagaaaaaa gaagcattga
    39721 agcaagcact cttgcatgaa aaacagtatc ttcaaaatga ggagtttgct gaattttatt
    39781 ttgacttgtt aatcagtaca caagattttt tatatgctag aaaattgata gcaagtacag
    39841 atttttatga agctttcgag cagcgggtca ttgaaaaaat ccaatttgca gaagaattat
    39901 ccggacaaat ggagaggcag aagatcaagg cactccacaa taaatcggag gaacttcctt
    39961 cgttggaacc tgcacgtcag cttgcgtcta ttgatgagat cgaacagctt ccttatcatg
    40021 agtttatcca aacagcgagt aaacttattg tatcgccaga ggtccatatt ttagcaaggg
    40081 caaaattact ggagacactt aggcaattga acgaatgcca tcctgttttc tatttgacga
    40141 ttgaagaaaa gttagtcaaa gtcataccaa aagatctgcc aaagccgcag cagcaggcaa
    40201 gttaccagca attatgtgta ttttcagatc gttacggtaa tgaagatgcg ctgcttagtt
    40261 ccgtgttaaa tgaagaattc acgttgcaat ctgcaattgt ttatcctgtc tatgatacat
    40321 acatcgaaga cccgaagcgc tggtttcaac tgactgtaga ggcgtgtaca ggaaaaacga
    40381 tgtatgaagg tacagaagat gaaaaacaag attttttaaa aaaaagagaa aaaattttgc
    40441 agcaaatgat cttcttccat tagattttat cagtgaaaaa gataaaaatg ttatgaaaaa
    40501 caaggattct atccattcaa tatgtttaca atttgaaagg gttagtgtac tatttaaagg
    40561 tatgcatgaa acgtttttga tgtataaaat gaatttctaa actcggaggg aattaaatga
    40621 ctgcaacatt tgaaaaaaaa ggcacgaatg atggtgtttt aaaatttacg attgcgcaag
    40681 acgaaatcca aaaaggatta acaactgcgt ttaataaagt gaaaaaaagc ttgaacgtac
    40741 ctggattccg taaagggaaa gtttctcgtc aagtattcaa ccgtatgtac ggtgaagaag
    40801 ctttatatga agatgcattg aatgctgtat taccagaagc ttatgaagct gctgtcaaag
    40861 aagctggcat cgatcctgtt tctcaaccaa aaatcgatgt tgaaagcatg gaaaaaggtc
    40921 aagactgggt aatcactgct gaagtaacag tgaaaccaga agtaaaatta ggacaatata
    40981 aagatctagt tgttgaaaaa caagaccgtg aagtaacaga tgctgacgtg gatgcacgta
    41041 tcaaacgtga acaagaagca caagctgaat tagttatcaa agaagatgct gcagctgaaa
    41101 atggcgatac agtagtaatc gacttcgaag gctttgtaga tggcgaagcg tttgatggcg
    41161 gaaaaggcga aaattactca cttgaattag gttcaggctc attcattcca ggttttgaag
    41221 atcaattagt tggtcataaa tcaggtgaaa gtctagatgt aaatgtaact ttcccagaag
    41281 attatcaagc agaagatctt gctggaaaag aagcagtctt caaagtgaca atccatgaag
    41341 taaaagcaaa agaagtgcct gaattagatg acgaattcgc aaaagatgtt gatgacgaag
    41401 tatcaacatt agacgaattg aaagaaaaat atcgcaaaga attagtagaa acaaaagaaa
    41461 aagcagcgga cgatgcaaaa gatgaagcag cgatccgtat ggctgttgaa aacgcagaaa
    41521 tcgttgaatt gccacacgtg atggttcatg atgaagtaca tcgttcaatg gacgaattct
    41581 tgaacaacat gcaacgtcaa ggtatctctc ctgaaatgta ctatcaatta actggttcta
    41641 cagaagaaga cttgcataaa caattcgaag gtgaagcaga aatgcgcaca agaacaaatc
    41701 ttgtgatcga agcaattgca gctgctgaaa atcttcaagc aacagaagaa gaaatcaatg
    41761 cagaaatcca agaattatca gaaacataca acatgccaat cgaacaaatc aaacgcgtgt
    41821 tgacagaaga tatgctggaa catgatgtga caatgaaaaa agcaatcgaa ttgatcacag
    41881 gtacagcgac agaagcgaaa gaatagaaat caagtaataa atcatttctg aagaagctac
    41941 ttctagaaaa ttgtttattc gaaataaggg actgtgacac gattttgtca cagttcctta
    42001 ttttttgtta agagtaaaaa atgcaaaggt ataggtcagt tgatcctttg ttaaacactt
    42061 tttcttttta ctaaactatg ttatgatagt aaaagactgt aaagtctttg atagtttctt
    42121 gcatactttt tgcaagttaa ggggtgaaga ccatgtatga caatccaagt agcaatgaga
    42181 ccgtacgctg ttctttttgc gggaaaaccc aagaagaagt gaaaaagatc gtagctggtc
    42241 caggggtcta catctgtaat gaatgtatcg acctatgtaa agaaatcatt gatgaagaat
    42301 tttatgaaga agctgtacgc gaattcactg aagtccctaa accactagaa attcttgaag
    42361 tattgaataa ctacgtgatt ggacaagatc gcgcaaaacg ctcgttagct gttgccgtat
    42421 acaatcacta taaacgtgtc aatcagcaag cacaagaaaa cacagatgat gttgaactgc
    42481 aaaaaagcaa tatctgcttg atcggaccta cagggtctgg taaaacattt ttagcacaaa
    42541 cattagcaaa aacattgaat gtaccttttg caatcgcaga tgcgacaagt ttgacagaag
    42601 ctggttatgt gggggaagat gtagaaaaca ttttgcttaa attgcttcaa tctgctgatt
    42661 acaatgtgga acgtgcagaa aaagggatca tctatattga tgagatcgat aaaattgcgc
    42721 gtaaaagcga aaatgtatct attactcgtg acgtttccgg cgaaggtgtc caacaagcct
    42781 tgctgaaaat tctagaagga actgttgcaa gcgtgccacc tcagggagga cgtaaacatc
    42841 cacatcaaga atttattcag attgatacga cgaacatatt gttcatcgta ggtggtgcat
    42901 ttgatggcat cgaaacgatt gtgaagaatc gtatgggcga aaagacgatt ggatttggta
    42961 cacaaaacca aaaactttct gaagacgaaa gtgtcatgca gcatatcatt cctgaagatt
    43021 tactaaaatt cggattgatc cctgagttta tcggacgatt acctgttatg gcagctttag
    43081 agaaattgac aacagatgat ctggtacgga tcttgactga gccgaaaaat gcgctagtca
    43141 aacaatatca aaaacttctt tctctagata ataccgaact tgaattcgaa cctgaagcgt
    43201 tgagagccat tgcgaaaaaa gcaatcgaac gcaataccgg tgctcgagga cttcgttcca
    43261 tcattgaaga aatcatgatg gatgtgatgt ttgatatccc ttcaaatgaa aacatcgaaa
    43321 aagtgattat cacaaaagaa gcagctgaat tgtctggtga gccaacagta atctataaag
    43381 attcagagaa aaaagcgggt tgatgaaaag aggctgccgt aaaagtcatt ttggcttttg
    43441 cgacagccgc ttgctttatt catcaccttg ttaaggagag agaccatgaa agtccatcaa
    43501 gctgaaatta tcataagtgc agtggcacca aaacaatatc cagatacaca attgccagaa
    43561 atagctctgg caggacgatc aaatgtaggg aaatcttctt ttatcaacac attgattgat
    43621 cgaaagaatt tagcccgtac ttccggtaag ccaggaaaga cccagacatt gaatttttat
    43681 ttgatcgaga acgcattaca ttttgtcgat gttccgggat atgggtatgc aaaagtatcc
    43741 aaaactgagc gagcaaaatg gggaaagatg atcgagacat atttaacgac tagagaccag
    43801 ctacgtgcgg tcgtttcttt agtagatttg cgtcataagc cttctcaaga agatatccag
    43861 atgtatcaat ttttgaagta ttatgatatc cctgtaattg ttgttgccac taaggcagat
    43921 aagatccctc gtggtaaatg gaacaagcat gagagcgaaa tcaaaaaagc gttggatttt
    43981 gatcgcaatg acacgtttat tctcttttct tccgttacaa aacagggaaa agaagaagcg
    44041 tgggcagcaa tcgaacaagc aattgaatca taaaaagaat aaaaaagact taccagtcaa
    44101 actctttcat aaaagtttgg ctggtaagtc tttttttgag cttaacttac ttttgtttct
    44161 tgtctttttt cttttcattt tgtggctgtg gttttagaaa tcgatcgatc tcttcttttg
    44221 gtgcaactgt tttttccgga tcgacagcaa atgattcgaa aagtttatct aaattatctg
    44281 tacgttcaaa tttcaacccc attgtaaaca cctctttgtt tctttttact tagtctatca
    44341 tgtaatcgtc caaattggtt tctaagacat aaagttttag tttttcttta tcgttggtta
    44401 taattaaatt ataaaaagat tttaaagagg tgagaaaatg attggcgaag ataaattatt
    44461 gatgactatc cagctcacag acttaaatga agaagaaaaa aaacaggaaa taagcgattg
    44521 ggctattttg acacgtctgt tgatccagcc aacatttatc cgctctttca cacaacaagc
    44581 ggatccgtat gatcttcgaa agcttcaaga aaaaataatg ctagcatcgg taagagacga
    44641 aagttggtta gttgtaggaa atgacgagaa cgaatgtagt tttcgcttgg aagataatca
    44701 attattgata aaaaatgtat tgtctatctc tgttttcaaa gaaaagcaat ttttgattcg
    44761 agacttcatt cagaaaaaaa tgattgctca tggggtgttc gcctatatgc gagcttatag
    44821 cgagtttatc tatcacaata caaaacagat ctctcaacgg ctattgtttg agaaaaagga
    44881 tgaaatcgaa catttaccta aaatgaaaca acaaaacggg gaagtagtag tcgattgcaa
    44941 ccaatttcca ggatacgatc ttttttacca aggtttatgt ttcacttcgt gttgggaaat
    45001 gtattatagt cgctattatc atcaaatcat ccctaaaccg atttttctcg acatccaaca
    45061 agtagaaaaa gtgaaggaat tagaaaacga agtcatatgt atccagcttt atcgagaccc
    45121 ctttaactgg caaaagccaa acaatcaaat gtttcaatcc tattttaggg accagcttgg
    45181 gtttgatcat cttgcttggg acaatggtgt cggtttattg aaaccgccat tcgttgaata
    45241 catttacact gaccatacga tccaatctgt ccagtatcag aatgcacaaa tgcaacctgt
    45301 tcctaaaaag aatgcttcat tttttgtaac aaaaagttat gatattcaac aaggagatta
    45361 taaagaaagg cgtgttcgcg gaacactaaa tgctcaggcg tattttcctt gggttgacga
    45421 gaatcgctcg cgcatgatgt gctataaagt tatcgatccg actgttgctt tggataatgg
    45481 aatcgaagct tattgttatt atattaggga atatctagaa gtagaggtaa cagatgaaaa
    45541 atatcaagag tatcttttat ccttaagaat ctatgttcct tcagaatccc tgtcagaatt
    45601 accgttaaag gaaatcaaac atagactctc tgatgttacc ttcaaacgaa taaagaaaaa
    45661 gcggcgaagt atccaattcg atgtcaagaa aggtgagcag catctgcgtg tgcaattttt
    45721 agattaccga gaattagaac aattgattac tttacaaaaa atatgaggtg aagaaaatgg
    45781 ataaacaact ctattatcga ataatggttc cggcatcatt attatccgtc tgtctctttt
    45841 ttgttcctaa ttttcctatt cgatcagtca tcggtttttt ggtcatgttt ggcgggatgg
    45901 gactttatta ttatcttctt cataaggaaa gaaaacctgc ttctgtttat atgaaaaaaa
    45961 caagaagcga aaataaaaaa cgataaaacg gcttgatgat agacaaaaaa ataaaaaacc
    46021 agtctttttg actggttttt tattt
//
LOCUS       DAZBWR010000008        45366 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000008 DAZBWR010000000
VERSION     DAZBWR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.008"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1722)
                     /locus_tag="ACHGT5_000426"
     CDS             complement(22..1722)
                     /locus_tag="ACHGT5_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216442.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="ACHGT5_000427"
     CDS             2229..3389
                     /locus_tag="ACHGT5_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN7216443.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="ACHGT5_000428"
     CDS             3405..3848
                     /locus_tag="ACHGT5_000428"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216444.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="ACHGT5_000429"
     CDS             complement(4331..4861)
                     /locus_tag="ACHGT5_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN7216445.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="ACHGT5_000430"
     CDS             4966..5517
                     /locus_tag="ACHGT5_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN7216446.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="ACHGT5_000431"
     CDS             complement(5670..6143)
                     /locus_tag="ACHGT5_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN7216447.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="ACHGT5_000432"
     CDS             6281..7753
                     /locus_tag="ACHGT5_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN7216448.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="ACHGT5_000433"
     CDS             7750..8460
                     /locus_tag="ACHGT5_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN7216449.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8812..9096
                     /locus_tag="ACHGT5_000434"
     CDS             8812..9096
                     /locus_tag="ACHGT5_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN7216450.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9413..9796)
                     /locus_tag="ACHGT5_000435"
     CDS             complement(9413..9796)
                     /locus_tag="ACHGT5_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN7216451.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9798..10382)
                     /locus_tag="ACHGT5_000436"
     CDS             complement(9798..10382)
                     /locus_tag="ACHGT5_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN7216452.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10429..11904)
                     /locus_tag="ACHGT5_000437"
     CDS             complement(10429..11904)
                     /locus_tag="ACHGT5_000437"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN7216453.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12085..12396
                     /locus_tag="ACHGT5_000438"
     CDS             12085..12396
                     /locus_tag="ACHGT5_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216454.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12511..14847)
                     /locus_tag="ACHGT5_000439"
     CDS             complement(12511..14847)
                     /locus_tag="ACHGT5_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN7216455.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14989..15657
                     /locus_tag="ACHGT5_000440"
     CDS             14989..15657
                     /locus_tag="ACHGT5_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7216456.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15660..16703
                     /locus_tag="ACHGT5_000441"
     CDS             15660..16703
                     /locus_tag="ACHGT5_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7216457.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16940..17821)
                     /locus_tag="ACHGT5_000442"
     CDS             complement(16940..17821)
                     /locus_tag="ACHGT5_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN7216458.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17980..18708
                     /locus_tag="ACHGT5_000443"
     CDS             17980..18708
                     /locus_tag="ACHGT5_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN7216459.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18864..19181
                     /locus_tag="ACHGT5_000444"
     CDS             18864..19181
                     /locus_tag="ACHGT5_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN7216460.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19174..19782
                     /locus_tag="ACHGT5_000445"
     CDS             19174..19782
                     /locus_tag="ACHGT5_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN7216461.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19837..20628
                     /locus_tag="ACHGT5_000446"
     CDS             19837..20628
                     /locus_tag="ACHGT5_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN7216462.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20766..21254
                     /locus_tag="ACHGT5_000447"
     CDS             20766..21254
                     /locus_tag="ACHGT5_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN7216463.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21305..22177
                     /locus_tag="ACHGT5_000448"
     CDS             21305..22177
                     /locus_tag="ACHGT5_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN7216464.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22353..22715
                     /locus_tag="ACHGT5_000449"
     CDS             22353..22715
                     /locus_tag="ACHGT5_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN7216465.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22724..23476
                     /locus_tag="ACHGT5_000450"
     CDS             22724..23476
                     /locus_tag="ACHGT5_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216466.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23490..25292
                     /locus_tag="ACHGT5_000451"
     CDS             23490..25292
                     /locus_tag="ACHGT5_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216467.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25569..26609
                     /locus_tag="ACHGT5_000452"
     CDS             25569..26609
                     /locus_tag="ACHGT5_000452"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN7216468.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26661..28805)
                     /locus_tag="ACHGT5_000453"
     CDS             complement(26661..28805)
                     /locus_tag="ACHGT5_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN7216469.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29162..30535)
                     /locus_tag="ACHGT5_000454"
     CDS             complement(29162..30535)
                     /locus_tag="ACHGT5_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7216470.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACHGT5_000455"
     CDS             complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACHGT5_000455"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN7216471.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACHGT5_000456"
     CDS             complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACHGT5_000456"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN7216472.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31983..32996)
                     /locus_tag="ACHGT5_000457"
     CDS             complement(31983..32996)
                     /locus_tag="ACHGT5_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN7216473.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33077..33676)
                     /locus_tag="ACHGT5_000458"
     CDS             complement(33077..33676)
                     /locus_tag="ACHGT5_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN7216474.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33862..34563
                     /locus_tag="ACHGT5_000459"
     CDS             33862..34563
                     /locus_tag="ACHGT5_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216475.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34573..37284
                     /locus_tag="ACHGT5_000460"
     CDS             34573..37284
                     /locus_tag="ACHGT5_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN7216476.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37735..39363
                     /gene="treC"
                     /locus_tag="ACHGT5_000461"
     CDS             37735..39363
                     /gene="treC"
                     /locus_tag="ACHGT5_000461"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN7216477.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39423..40604)
                     /locus_tag="ACHGT5_000462"
     CDS             complement(39423..40604)
                     /locus_tag="ACHGT5_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN7216478.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40856..42379
                     /locus_tag="ACHGT5_000463"
     CDS             40856..42379
                     /locus_tag="ACHGT5_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN7216479.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42448..43176)
                     /locus_tag="ACHGT5_000464"
     CDS             complement(42448..43176)
                     /locus_tag="ACHGT5_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN7216480.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREATLANGETIPASIRGLKKLIS"
     gene            complement(43189..44469)
                     /locus_tag="ACHGT5_000465"
     CDS             complement(43189..44469)
                     /locus_tag="ACHGT5_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN7216481.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44610..45212)
                     /locus_tag="ACHGT5_000466"
     CDS             complement(44610..45212)
                     /locus_tag="ACHGT5_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN7216482.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
BASE COUNT        14529 a         8093 c         9065 g        13679 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca
     8521 atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc gtagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct gagtcacaag
     8641 gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt gaccgaggag
     8701 ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt tgtcacagtt
     8761 ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa aatgagggaa
     8821 atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg ggcatatatt
     8881 atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt ccaagcaact
     8941 tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt
     9001 tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg aaaagaaatc
     9061 ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt
     9121 cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     9181 caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg aagcggcttt
     9241 atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg
     9301 gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca taacgctgat
     9361 ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact ggttatttga
     9421 tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct tctgacatcg
     9481 gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg gaagtgatcg
     9541 tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct aagacatcgt
     9601 ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct gtatcacgag
     9661 aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg atcaataagt
     9721 ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt acttcaacta
     9781 tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt tacaatttca
     9841 aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt ttgaggctga
     9901 ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag
     9961 ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa ctttcgttgc
    10021 tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc aacagagata
    10081 cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa aaatgatccg
    10141 ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag gctttcgaat
    10201 tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg actgatgatt
    10261 ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat ggcataaatg
    10321 gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc caattgttcc
    10381 attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt atttcaaata
    10441 tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt tcatatcgaa
    10501 aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa tagagccagg
    10561 attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg ggatcacgat
    10621 gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa
    10681 aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc
    10741 aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac ggatttgcat
    10801 gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta cttttcctgg
    10861 aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg ccatagatac
    10921 gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc ggtgagtaac
    10981 tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc caggaaattc
    11041 tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat ttggcatgat
    11101 ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg ctataccatc
    11161 aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat tgtagccaag
    11221 tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta aaccaatatt
    11281 gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg
    11341 gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa ccatacgatg
    11401 gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac ccattgtgat
    11461 gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa
    11521 gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt
    11581 cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc
    11641 atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt tcacacgtgt
    11701 gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag tcggacgttt
    11761 tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt caggatcgat
    11821 ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga tgacttttct
    11881 taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta cctatctata
    11941 gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc tacaatggaa
    12001 tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa aagaaagcgc
    12061 atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc taaagcagta
    12121 aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga agtaaaatac
    12181 gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa aaaaggaaga
    12241 ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac tgaagtaacg
    12301 attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc agaagagttg
    12361 tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa aaacagcgcg
    12421 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    12481 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    12541 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    12601 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    12661 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    12721 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    12781 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    12841 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    12901 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    12961 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    13021 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    13081 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    13141 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    13201 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    13261 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    13321 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    13381 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    13441 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    13501 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    13561 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    13621 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    13681 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    13741 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    13801 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    13861 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    13921 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    13981 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    14041 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    14101 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    14161 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    14221 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    14281 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    14341 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    14401 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    14461 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    14521 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    14581 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    14641 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    14701 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    14761 tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata
    14821 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    14881 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    14941 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    15001 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    15061 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    15121 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    15181 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    15241 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    15301 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    15361 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    15421 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    15481 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    15541 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    15601 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    15661 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    15721 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    15781 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    15841 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    15901 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    15961 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    16021 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    16081 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    16141 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    16201 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    16261 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    16321 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    16381 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    16441 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    16501 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    16561 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    16621 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    16681 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    16741 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    16801 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    16861 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    16921 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    16981 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    17041 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    17101 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    17161 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    17221 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    17281 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    17341 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    17401 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    17461 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    17521 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    17581 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    17641 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    17701 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    17761 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    17821 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    17881 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    17941 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    18001 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    18061 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    18121 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    18181 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    18241 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    18301 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    18361 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    18421 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    18481 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    18541 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    18601 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    18661 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    18721 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    18781 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    18841 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    18901 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    18961 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    19021 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    19081 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    19141 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    19201 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    19261 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    19321 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    19381 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    19441 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    19501 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    19561 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    19621 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    19681 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    19741 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    19801 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    19861 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    19921 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    19981 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    20041 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    20101 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    20161 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    20221 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    20281 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    20341 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    20401 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    20461 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    20521 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    20581 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    20641 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    20701 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    20761 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    20821 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    20881 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    20941 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    21001 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    21061 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    21121 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    21181 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    21241 gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag
    21301 tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt
    21361 cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg
    21421 ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac
    21481 tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat
    21541 tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa
    21601 agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga
    21661 aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc
    21721 gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca
    21781 atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt
    21841 gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat
    21901 cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg
    21961 tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat
    22021 cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg
    22081 gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc
    22141 tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag
    22201 aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga
    22261 caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa
    22321 tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg
    22381 ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag
    22441 aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg
    22501 gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa
    22561 aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa
    22621 tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc
    22681 cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga
    22741 agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag
    22801 cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc
    22861 tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag atgggcatat
    22921 ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc
    22981 gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga
    23041 aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt
    23101 ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat
    23161 ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat
    23221 tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga
    23281 tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc
    23341 gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga
    23401 agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg
    23461 aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt actgggatca
    23521 agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt
    23581 tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg
    23641 cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt
    23701 atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta
    23761 tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag
    23821 tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc
    23881 accgattatt agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc
    23941 acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca
    24001 atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa
    24061 caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc
    24121 taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg
    24181 tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct
    24241 ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat
    24301 tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc
    24361 tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag
    24421 acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc
    24481 agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg
    24541 cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac
    24601 atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg
    24661 aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa
    24721 tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc
    24781 tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa
    24841 ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt
    24901 atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag
    24961 ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg
    25021 acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat
    25081 cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg
    25141 tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg
    25201 ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat
    25261 atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga
    25321 acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg
    25381 caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc
    25441 tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag
    25501 ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg
    25561 caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca
    25621 cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc
    25681 aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat
    25741 atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga cggattacga
    25801 atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc
    25861 attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca
    25921 atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat
    25981 ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag
    26041 tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt
    26101 tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc
    26161 gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg
    26221 gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc
    26281 gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt
    26341 tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag
    26401 atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg
    26461 ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg
    26521 agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa
    26581 cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca
    26641 catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct
    26701 aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct
    26761 ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg
    26821 gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca
    26881 tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    26941 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    27001 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    27061 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    27121 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    27181 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    27241 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    27301 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    27361 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    27421 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct
    27481 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    27541 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    27601 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    27661 gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta
    27721 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    27781 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    27841 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    27901 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    27961 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    28021 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    28081 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    28141 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    28201 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    28261 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    28321 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    28381 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    28441 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    28501 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    28561 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    28621 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    28681 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    28741 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    28801 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    28861 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    28921 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    28981 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    29041 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    29101 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    29161 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    29221 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    29281 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    29341 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    29401 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    29461 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    29521 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    29581 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    29641 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    29701 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    29761 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    29821 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    29881 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    29941 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    30001 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    30061 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    30121 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    30181 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    30241 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    30301 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    30361 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    30421 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    30481 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    30541 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    30601 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    30661 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    30721 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    30781 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    30841 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    30901 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    30961 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    31021 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    31081 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    31141 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    31201 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    31261 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    31321 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    31381 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    31441 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    31501 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    31561 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    31621 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    31681 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    31741 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    31801 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    31861 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    31921 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    31981 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    32041 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    32101 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    32161 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    32221 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    32281 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    32341 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    32401 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    32461 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    32521 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    32581 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    32641 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    32701 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    32761 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    32821 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    32881 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    32941 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    33001 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    33061 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    33121 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    33181 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    33241 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    33301 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    33361 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    33421 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    33481 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    33541 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    33601 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    33661 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    33721 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    33781 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    33841 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    33901 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    33961 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    34021 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    34081 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    34141 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    34201 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    34261 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    34321 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    34381 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    34441 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    34501 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    34561 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    34621 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    34681 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    34741 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    34801 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    34861 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    34921 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    34981 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    35041 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    35101 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    35161 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    35221 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    35281 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    35341 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    35401 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    35461 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    35521 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    35581 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    35641 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    35701 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    35761 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    35821 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    35881 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    35941 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    36001 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    36061 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    36121 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    36181 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    36241 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    36301 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    36361 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    36421 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    36481 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    36541 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    36601 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    36661 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    36721 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    36781 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    36841 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    36901 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    36961 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    37021 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    37081 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    37141 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    37201 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    37261 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt
    37321 attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg
    37381 aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc
    37441 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    37501 gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa
    37561 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    37621 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    37681 ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    37741 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    37801 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    37861 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    37921 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    37981 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    38041 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    38101 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    38161 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    38221 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    38281 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    38341 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    38401 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    38461 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    38521 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    38581 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    38641 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    38701 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    38761 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    38821 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    38881 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    38941 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    39001 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    39061 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    39121 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    39181 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    39241 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    39301 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    39361 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    39421 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
    39481 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    39541 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    39601 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    39661 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    39721 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    39781 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    39841 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    39901 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    39961 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    40021 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    40081 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    40141 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    40201 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    40261 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
    40321 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    40381 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    40441 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    40501 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    40561 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
    40621 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    40681 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
    40741 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
    40801 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    40861 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    40921 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    40981 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    41041 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    41101 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    41161 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    41221 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
    41281 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
    41341 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    41401 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    41461 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
    41521 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
    41581 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    41641 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
    41701 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    41761 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
    41821 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
    41881 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
    41941 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    42001 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    42061 gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt
    42121 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    42181 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    42241 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
    42301 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    42361 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    42421 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    42481 ggctgggatg gtctcgccgt ttgccaacgt ggcttctctg gtttttttat taatcgaccg
    42541 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    42601 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    42661 tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    42721 gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc
    42781 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt
    42841 gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg
    42901 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    42961 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    43021 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    43081 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    43141 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    43201 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    43261 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    43321 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
    43381 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    43441 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    43501 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
    43561 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    43621 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    43681 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    43741 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    43801 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    43861 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    43921 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    43981 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    44041 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
    44101 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
    44161 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    44221 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    44281 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    44341 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    44401 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    44461 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
    44521 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    44581 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    44641 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    44701 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
    44761 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    44821 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    44881 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    44941 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    45001 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    45061 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    45121 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    45181 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    45241 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    45301 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    45361 tgatac
//
LOCUS       DAZBWR010000009        44740 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000009 DAZBWR010000000
VERSION     DAZBWR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.009"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1008)
                     /locus_tag="ACHGT5_000467"
     CDS             complement(<1..1008)
                     /locus_tag="ACHGT5_000467"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN7216483.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="ACHGT5_000468"
     CDS             complement(1188..1532)
                     /locus_tag="ACHGT5_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN7216484.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="ACHGT5_000469"
     CDS             complement(1525..1959)
                     /locus_tag="ACHGT5_000469"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN7216485.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="ACHGT5_000470"
     CDS             2097..2834
                     /locus_tag="ACHGT5_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216486.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="ACHGT5_000471"
     CDS             2827..4038
                     /locus_tag="ACHGT5_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216487.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4242..5024
                     /locus_tag="ACHGT5_000472"
     CDS             4242..5024
                     /locus_tag="ACHGT5_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN7216488.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="ACHGT5_000473"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="ACHGT5_000473"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN7216489.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACHGT5_000474"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACHGT5_000474"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN7216490.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="comGA"
                     /locus_tag="ACHGT5_000475"
     CDS             7740..8669
                     /gene="comGA"
                     /locus_tag="ACHGT5_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN7216491.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /gene="comGB"
                     /locus_tag="ACHGT5_000476"
     CDS             8626..9675
                     /gene="comGB"
                     /locus_tag="ACHGT5_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN7216492.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /gene="comGC"
                     /locus_tag="ACHGT5_000477"
     CDS             9672..9950
                     /gene="comGC"
                     /locus_tag="ACHGT5_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN7216493.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /gene="comGD"
                     /locus_tag="ACHGT5_000478"
     CDS             9925..10395
                     /gene="comGD"
                     /locus_tag="ACHGT5_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN7216494.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10367..10714
                     /locus_tag="ACHGT5_000479"
     CDS             10367..10714
                     /locus_tag="ACHGT5_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216495.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10677..11102
                     /gene="comGF"
                     /locus_tag="ACHGT5_000480"
     CDS             10677..11102
                     /gene="comGF"
                     /locus_tag="ACHGT5_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN7216496.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /gene="comGG"
                     /locus_tag="ACHGT5_000481"
     CDS             11074..11433
                     /gene="comGG"
                     /locus_tag="ACHGT5_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN7216497.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="ACHGT5_000482"
     CDS             11605..12612
                     /locus_tag="ACHGT5_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7216498.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="ACHGT5_000483"
     CDS             12637..13821
                     /locus_tag="ACHGT5_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN7216499.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACHGT5_000484"
     CDS             complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACHGT5_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN7216500.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15946..16074)
                     /locus_tag="ACHGT5_000485"
     CDS             complement(15946..16074)
                     /locus_tag="ACHGT5_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216501.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16419..16562)
                     /locus_tag="ACHGT5_000486"
     CDS             complement(16419..16562)
                     /locus_tag="ACHGT5_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN7216502.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="ACHGT5_000487"
     CDS             complement(16785..17264)
                     /locus_tag="ACHGT5_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN7216503.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="ACHGT5_000488"
     CDS             complement(17337..17621)
                     /locus_tag="ACHGT5_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216504.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="ACHGT5_000489"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHGT5_000489"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="ACHGT5_000490"
     CDS             complement(17965..19251)
                     /locus_tag="ACHGT5_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN7216505.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="ACHGT5_000491"
     CDS             19389..20060
                     /locus_tag="ACHGT5_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN7216506.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="ACHGT5_000492"
     CDS             20026..20514
                     /locus_tag="ACHGT5_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN7216507.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="ACHGT5_000493"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="ACHGT5_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN7216508.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="ACHGT5_000494"
     CDS             21475..22239
                     /locus_tag="ACHGT5_000494"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN7216509.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="ACHGT5_000495"
     CDS             22472..24685
                     /locus_tag="ACHGT5_000495"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN7216510.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /gene="dtd"
                     /locus_tag="ACHGT5_000496"
     CDS             24696..25142
                     /gene="dtd"
                     /locus_tag="ACHGT5_000496"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN7216511.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="ACHGT5_000497"
     CDS             25313..26572
                     /locus_tag="ACHGT5_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN7216512.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="ACHGT5_000498"
     CDS             complement(26661..27608)
                     /locus_tag="ACHGT5_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN7216513.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /gene="hisS"
                     /locus_tag="ACHGT5_000499"
     CDS             28002..29294
                     /gene="hisS"
                     /locus_tag="ACHGT5_000499"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN7216514.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="ACHGT5_000500"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="ACHGT5_000500"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN7216515.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="ACHGT5_000501"
     CDS             31187..32062
                     /locus_tag="ACHGT5_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN7216516.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="ACHGT5_000502"
     CDS             32328..34211
                     /locus_tag="ACHGT5_000502"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN7216517.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="ACHGT5_000503"
     CDS             complement(34286..34570)
                     /locus_tag="ACHGT5_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN7216518.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="ACHGT5_000504"
     CDS             complement(34560..35294)
                     /locus_tag="ACHGT5_000504"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN7216519.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="ACHGT5_000505"
     CDS             35423..36046
                     /locus_tag="ACHGT5_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN7216520.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="ACHGT5_000506"
     CDS             36238..37359
                     /locus_tag="ACHGT5_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN7216521.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="ACHGT5_000507"
     CDS             37366..40494
                     /locus_tag="ACHGT5_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7216522.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="ACHGT5_000508"
     CDS             40513..43707
                     /locus_tag="ACHGT5_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN7216523.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="ACHGT5_000509"
     CDS             complement(43853..44560)
                     /locus_tag="ACHGT5_000509"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7216524.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14352 a         7952 c         9393 g        13043 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgag aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggt
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata
//
LOCUS       DAZBWR010000010        43304 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000010 DAZBWR010000000
VERSION     DAZBWR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.010"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..2632
                     /gene="adhE"
                     /locus_tag="ACHGT5_000510"
                     /gene_synonym="adhC"
     CDS             35..2632
                     /gene="adhE"
                     /locus_tag="ACHGT5_000510"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN7216525.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2861..3967
                     /locus_tag="ACHGT5_000511"
     CDS             2861..3967
                     /locus_tag="ACHGT5_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN7216526.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3984..5012
                     /locus_tag="ACHGT5_000512"
     CDS             3984..5012
                     /locus_tag="ACHGT5_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN7216527.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5018..5497
                     /locus_tag="ACHGT5_000513"
     CDS             5018..5497
                     /locus_tag="ACHGT5_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN7216528.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5513..6808
                     /locus_tag="ACHGT5_000514"
     CDS             5513..6808
                     /locus_tag="ACHGT5_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN7216529.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6819..7643
                     /locus_tag="ACHGT5_000515"
     CDS             6819..7643
                     /locus_tag="ACHGT5_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN7216530.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7808..8707
                     /locus_tag="ACHGT5_000516"
     CDS             7808..8707
                     /locus_tag="ACHGT5_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216531.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8833..9702)
                     /locus_tag="ACHGT5_000517"
     CDS             complement(8833..9702)
                     /locus_tag="ACHGT5_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN7216532.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9855..11315
                     /gene="rhaB"
                     /locus_tag="ACHGT5_000518"
     CDS             9855..11315
                     /gene="rhaB"
                     /locus_tag="ACHGT5_000518"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN7216533.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11312..12568
                     /gene="rhaA"
                     /locus_tag="ACHGT5_000519"
     CDS             11312..12568
                     /gene="rhaA"
                     /locus_tag="ACHGT5_000519"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN7216534.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12582..13427
                     /gene="rhaD"
                     /locus_tag="ACHGT5_000520"
     CDS             12582..13427
                     /gene="rhaD"
                     /locus_tag="ACHGT5_000520"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN7216535.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13429..13740
                     /gene="rhaM"
                     /locus_tag="ACHGT5_000521"
     CDS             13429..13740
                     /gene="rhaM"
                     /locus_tag="ACHGT5_000521"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN7216536.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13822..14973
                     /gene="fucO"
                     /locus_tag="ACHGT5_000522"
     CDS             13822..14973
                     /gene="fucO"
                     /locus_tag="ACHGT5_000522"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN7216537.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15100..15858
                     /locus_tag="ACHGT5_000523"
     CDS             15100..15858
                     /locus_tag="ACHGT5_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN7216538.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15958..16602
                     /locus_tag="ACHGT5_000524"
     CDS             15958..16602
                     /locus_tag="ACHGT5_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN7216539.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16609..17616
                     /locus_tag="ACHGT5_000525"
     CDS             16609..17616
                     /locus_tag="ACHGT5_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN7216540.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17632..18474
                     /gene="kduI"
                     /locus_tag="ACHGT5_000526"
     CDS             17632..18474
                     /gene="kduI"
                     /locus_tag="ACHGT5_000526"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN7216541.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18494..19297
                     /locus_tag="ACHGT5_000527"
     CDS             18494..19297
                     /locus_tag="ACHGT5_000527"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN7216542.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19546..20610)
                     /gene="fni"
                     /locus_tag="ACHGT5_000528"
     CDS             complement(19546..20610)
                     /gene="fni"
                     /locus_tag="ACHGT5_000528"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN7216543.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20607..21692)
                     /locus_tag="ACHGT5_000529"
     CDS             complement(20607..21692)
                     /locus_tag="ACHGT5_000529"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN7216544.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21705..22682)
                     /gene="mvaD"
                     /locus_tag="ACHGT5_000530"
     CDS             complement(21705..22682)
                     /gene="mvaD"
                     /locus_tag="ACHGT5_000530"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN7216545.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22675..23619)
                     /gene="mvk"
                     /locus_tag="ACHGT5_000531"
     CDS             complement(22675..23619)
                     /gene="mvk"
                     /locus_tag="ACHGT5_000531"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN7216546.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23935..24594
                     /locus_tag="ACHGT5_000532"
     CDS             23935..24594
                     /locus_tag="ACHGT5_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN7216547.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24679..25584
                     /locus_tag="ACHGT5_000533"
     CDS             24679..25584
                     /locus_tag="ACHGT5_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN7216548.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25586..26218)
                     /locus_tag="ACHGT5_000534"
     CDS             complement(25586..26218)
                     /locus_tag="ACHGT5_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN7216549.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26369..26494
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26538..27062
                     /gene="infC"
                     /locus_tag="ACHGT5_000535"
     CDS             26538..27062
                     /gene="infC"
                     /locus_tag="ACHGT5_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN7216550.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27134..27334
                     /gene="rpmI"
                     /locus_tag="ACHGT5_000536"
     CDS             27134..27334
                     /gene="rpmI"
                     /locus_tag="ACHGT5_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN7216551.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27387..27746
                     /gene="rplT"
                     /locus_tag="ACHGT5_000537"
     CDS             27387..27746
                     /gene="rplT"
                     /locus_tag="ACHGT5_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN7216552.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            27998..29335
                     /locus_tag="ACHGT5_000538"
     CDS             27998..29335
                     /locus_tag="ACHGT5_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN7216553.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29383..31512
                     /locus_tag="ACHGT5_000539"
     CDS             29383..31512
                     /locus_tag="ACHGT5_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN7216554.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31487..32176
                     /locus_tag="ACHGT5_000540"
     CDS             31487..32176
                     /locus_tag="ACHGT5_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216555.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32584..33197)
                     /locus_tag="ACHGT5_000541"
                     /pseudo
     CDS             complement(32584..33197)
                     /locus_tag="ACHGT5_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33333..33527)
                     /locus_tag="ACHGT5_000542"
     CDS             complement(33333..33527)
                     /locus_tag="ACHGT5_000542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216556.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33577..34017)
                     /locus_tag="ACHGT5_000543"
     CDS             complement(33577..34017)
                     /locus_tag="ACHGT5_000543"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN7216557.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34021..34866)
                     /locus_tag="ACHGT5_000544"
     CDS             complement(34021..34866)
                     /locus_tag="ACHGT5_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN7216558.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35015..36433
                     /gene="pepV"
                     /locus_tag="ACHGT5_000545"
     CDS             35015..36433
                     /gene="pepV"
                     /locus_tag="ACHGT5_000545"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN7216559.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36490..37437)
                     /locus_tag="ACHGT5_000546"
     CDS             complement(36490..37437)
                     /locus_tag="ACHGT5_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN7216560.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37660..38010)
                     /locus_tag="ACHGT5_000547"
     CDS             complement(37660..38010)
                     /locus_tag="ACHGT5_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN7216561.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38210..39289
                     /gene="pepA"
                     /locus_tag="ACHGT5_000548"
     CDS             38210..39289
                     /gene="pepA"
                     /locus_tag="ACHGT5_000548"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN7216562.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39433..39753
                     /locus_tag="ACHGT5_000549"
     CDS             39433..39753
                     /locus_tag="ACHGT5_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN7216563.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39775..40239
                     /locus_tag="ACHGT5_000550"
     CDS             39775..40239
                     /locus_tag="ACHGT5_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN7216564.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40444..41049
                     /gene="ytpR"
                     /locus_tag="ACHGT5_000551"
     CDS             40444..41049
                     /gene="ytpR"
                     /locus_tag="ACHGT5_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN7216565.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41088..42377)
                     /locus_tag="ACHGT5_000552"
     CDS             complement(41088..42377)
                     /locus_tag="ACHGT5_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN7216566.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42805..43284
                     /gene="rlmH"
                     /locus_tag="ACHGT5_000553"
     CDS             42805..43284
                     /gene="rlmH"
                     /locus_tag="ACHGT5_000553"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN7216567.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        13681 a         7921 c         9293 g        12409 t
ORIGIN      
        1 taacaaataa ccatgaccca ttaggaggat aatgatggct aaagtaatag aaaaagaaaa
       61 aacgaagaca attgacgtac aagcaatgat tgatgatttg gcagaaaaag caaatgcagc
      121 attgaaagaa atggaaagct ttgatcaaga aaaagtagac catatcgtgc atgaaatggc
      181 aatggcagct ttggatcaac atatgccatt agcgaagatg gctgtagagg aaacaggccg
      241 cggtgtttac gaagataaag caatcaaaaa tatgtatgcg tccgaatata tttggcataa
      301 tatcaaacat gataaaacag ttggtgtgat caatgaagat gtccaaaaag ggttgattga
      361 aattgctgaa ccagtcggtg tggtttgcgg agtaactcca acaacgaatc cgacttccac
      421 aacgattttc aaatcaatga tcgcgttgaa aacaagaaat ccaatcgttt ttgctttcca
      481 tccaagtgcc caaaaatctt ctgcagaagc agcacgagtg gtacgtgatg ctgctatcgc
      541 tgccggagct cctgaaaatt gtatccaatg gatcgaacat ccttcgatcg aagcgacttc
      601 tatgctaatg aaccatccag gtatcgcgat cgtcttagcg acaggtggag ctggaatggt
      661 caagtctgct tactctacag gaaaaccagc tttaggtgta ggtccaggta acgtaccagc
      721 ttatattgaa aaaacagcaa aaattaaacg tgcagtgaac gatttgatcg tttcgaaaac
      781 atttgataat ggaatgattt gtgcttctga acaagctgtg atcgtggata aagaaatcta
      841 tgcagcagta aaagcagaat tccaagcaca ccaagtttat atcgtaaaac cagatgaatt
      901 gcaaaaatta gaagatgcag tgatgaacga aggaaaatat gcggtcaatc catcaatcgt
      961 tggacattct gcgatggaaa tcgcaaaatt ggcaggaatc agtgtaccaa aaggaacgaa
     1021 aatgctgatt gctgaattgg aaggcgtagg cccagattat ccgctatcaa gagaaaaact
     1081 ttctccagtt cttgccatga tcaaagctaa caacacggac catgcgtttg atttgtgtga
     1141 aggtatgttg aatcttggag gattaggaca tacagcagtt atccattcag aaaacgaaga
     1201 gttgcatgtc aaattcggtc ttcgaatgaa agcttgtcgt attttagtga atacaccatc
     1261 tgcagaaggc ggcatcggtg atatctataa cgaaatgatt ccgtcattga ctttgggatg
     1321 cggttcttat ggtaagaact ctgtttctcg taacgtctct gctgtaaact taatcaatgt
     1381 caaaactgta gcgaaacgga gaaataacat gcaatggttc aaattaccgc caaaaatctt
     1441 ctttgaaaag aactctctat tgtacttaga gaaaatggaa aacgttgaac gtgtcatgat
     1501 cgtttgtgac cctggtatgg tccaattcgg ttactgcgat acagttcgtg aagtattggc
     1561 acgacgtaaa aatgatgtga agatcgaagt cttctcagat gttgaaccga acccatcgac
     1621 aaataccgta tacgcaggaa cgaaactgat ggcagacttc aagccagata cagtaatcgc
     1681 tcttggaggc ggttctgcaa tggacgcagc taagggaatg tggatgttct atgaacaccc
     1741 agacacatct ttctttggtg caaaacaaaa attcttggat atccgtaaac ggacatataa
     1801 gatcgacaaa ccagaaaaaa cacaattcgt atgtatcccg acaacatcag ggactggttc
     1861 tgaagtaacg ccatttgcgg tcatcactga cagtgaaaca catgtgaaat atccgttagc
     1921 agattatgca ttgacgccag atgtcgcaat cgtcgatcct caattcgtca tgtcagttcc
     1981 agcatctgtc acagcagata ccgggatgga tgtgttaaca catgcaatcg aatcctatgt
     2041 ttcagtcatg gcttcagatt atacacgtgg attgagccta caagcaatca agttagtctt
     2101 tgactattta gaaaaatcag taaaaacacc tgatatggaa tcacgtgaaa aaatgcacaa
     2161 cgcttctaca atggctggta tggcgtttgc taacgcattc ctaggtattt gtcactcagt
     2221 cgcacataaa atcggcgggg aatacgggat ccctcatgga cggacaaatg cgattctttt
     2281 accgcatatt atccgctata atgcaaaaga tccacaaaaa catgcgatgt tccctaaata
     2341 tgactatttc cgtgcagata cagattacgc agatatcgca aaattcttag gtctgaaagg
     2401 cgaaacaaca gaggaattag tcgaagcttt agcaacggct gtttatgaat taggaaaatc
     2461 tgtcggcatc aacatgagtc taaaagctca aggagtcaca caagaaacac tagatacaac
     2521 cgtagaccgt atggctgaat tagcttacga agaccaatgt acgacagcta atccgaaaga
     2581 accattgatc tctgaattga aacagatcat cattgatgct tacaacggat aagcaaaaga
     2641 aagcgtagaa aaaagataag aaaatagatg aaaaagaact tggaaaacgt tgtttctcca
     2701 agttcttttt ttaatttcca agtaactgaa agttactttt gttatttaac atttaggtca
     2761 attttagaca gtttttgttc aaaaagcgat atttatagtg gacggtatta ctgctatatt
     2821 aaggatataa aatgaaagcg tttaaaaagg tggcgtaaag atgattatta aagaagagat
     2881 ttttgtacct aaagaattag tgaaagaaaa aattgacctg ctcgttgaaa acctgacaac
     2941 gatcaaagac gataatggtg aatttttgtt ggactttgat ggactgaaag tagatgacaa
     3001 aagctggaca gtgtggaatt ggccacaagg tgtcggtcta tatggtattt ataaaaatta
     3061 ccagatgaca aaaaatcctc gcgcttatca agtggtcaat gaatggtttg aggatcgtat
     3121 ggaagaaggc gctccgccca aaaacgtcaa tacaatggca ccgctattga ctatggctta
     3181 cttgtatgaa gacacgaaag attcacgcta tttgccttat ttagaacaat gggcagaatg
     3241 ggtcatgaat gacatgcctc ggacaaatga agacgggtta cagcatgcca catatggacc
     3301 ggaaaataag aatcagttgt gggacgatac attgatgatg acagtgcttc ctttggcaaa
     3361 aatcgggaaa ctgttgaatc gcccagaata tttagaagaa gctcgttacc agtttatgat
     3421 ccatatcaaa tacttgatgg ataaacgaac aggattgtgg tatcacggct ggacgtttga
     3481 aggaaatcat aactatgccg aagcattttg ggctcgtgga aactgctggt tgacgattgc
     3541 tattccagag atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt
     3601 gatcgaaaca cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt
     3661 atggcatacg ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt
     3721 tgcttacggt attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt
     3781 cgcttatcgt gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa
     3841 cgtttcaatc gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac
     3901 agcaatgcct tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta
     3961 ttgttaaaag aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc
     4021 ttagcctagg tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg
     4081 cagatgcaga tgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagc
     4141 cagtaattga tgcaatgaat gaatttgggc gtttagtaaa agaaaaaaca gatggttccg
     4201 tacagatcca gtatttccca gatggacaac tgggtggaga acgtgaactg atcgaattga
     4261 cacagtcagg agcagtagat ttcacaaaag taagtgcctc agcgttggaa agtttctcta
     4321 aagattattc gattttttca gttccttatc tattcaatga tgaagaacat ttctttaagg
     4381 tgatggataa tgaagaaata atggagccaa tctaccaatc gaccaaaaat ttaggattta
     4441 ccggattgac ctattatgat tctggtcagc gcagctttta tatggtagac ggtccgatcc
     4501 atacgccgga agatttggag ggaaagaaga tccgtgtgat gcaaagcgaa accgctatca
     4561 aaatggtaga gcttttaggc ggttcgcccg tacctatggg aagtgatgaa gtgtacacgt
     4621 ctcttcaatc caatttgatc aacggatcag aaaacaatga atttgtcttg tatacagctg
     4681 gacatggtgg tgtggctaaa tactattcct atgatgagca tacacgtgtg ccagatatca
     4741 tcatcatgaa tgatgccatc aaagaacagc taactgatga gcaggaaaaa gccattgaag
     4801 aagcagcgaa agaatcaacg gtttttgaaa ttgaagcttt tgcaaaagca attgaagaag
     4861 aaaaagcttt agcacaagaa gaacatggag tccaattcaa tgatgtagat aatacaccgt
     4921 ttcgagaggc agttgcccca cttcatgata tattcaaaaa cgatcctacc tacaaagacc
     4981 tttatcaaaa gatccaagag caaggagcgt agctgtgatg aaaatgattt taaacaaggt
     5041 attagagatc ctgggagctg ttttgcttgt tgggatggtc gctgttgttt tatggcaagt
     5101 attttccaga acggttttag gaaatccaaa tacggtcacg gaagagcttg ttcgattcgg
     5161 tcttgtctgg tttgccatgt tgtcttccgc gtatgttgtg ggacaaaaag cacatttagc
     5221 cgtaacggtt ttaagtgaaa aattaactgg caaaaaaagt aaaattttag aagttgttgt
     5281 tcaaagttta tttctggtat ttgctgcgat gatcatggtg tatggcggat ggaatgctgt
     5341 cacattgaca atggggcaga tctcgccttc tttaagtatt cctatgggat atgtttatct
     5401 gtcagtacct gtatcggggg tactcatcat tatctatagc ctgatcaatc taatcgatac
     5461 attaagcaac aagccagcaa ttcaagaaaa aatataaaaa tggaggaata aaatggcaat
     5521 agaagccgga ttagtgttat tttttgtatt tgttgtgttg cttgtattag ggatgccgat
     5581 tgctataagt attgcattct catcgatgat gactttattg ttagttatac cgtttgatgt
     5641 ttctgctttt agttcagctc aaaaaatggt gggaagtatc aatagttttt cgctgatcgc
     5701 tattccgttt ttcgttctct cgggcattat catgaataat ggagggatag ctaagaaact
     5761 agtggatttt gcgatgttgt tcgtcgggcg cgtgccgggg gctttggctc atacgaatgt
     5821 gttagggaat gctttatttg gttccatctc tagctcggcc attgccgctt ctaccgcgat
     5881 tggcggagtg ttgatccctc agcaagtgga agaaggatat gaccggaagt ttgcgacagc
     5941 ggtcaatatc gcgtcagctc cgacaggtat ggtcattcct ccaagtactg cttttatcat
     6001 gtattcactt gttgctggtg gtgcttcgat ctctgctttg tttatgggag gttatctcgt
     6061 aggcgctttg tgggcgctag gaatcatgct agtagcattc atcgttgcta aaaagaataa
     6121 ataccctgtt gtcgaaaaag gacagatggc tcatgctgga aaaataataa aagaggcgat
     6181 tcctagcctt ctattgattg tcatcattat cggtggtatc ttgacgggga tatttacagc
     6241 agtagaagct tcagcgatag ctgtcattta ctccctgttg attgctatgt tttactataa
     6301 aacggtaaaa ctaaaagata tacctaatat gttgtttcaa tcagtagcaa tgtcagggac
     6361 gatcatgttc ttattggcta cttcttccat gatgtctttc gccatggcgt ttaccggtat
     6421 tcctcaggca attagttcgt tgattatggg cgtaacggat aataaattta ttattctgtt
     6481 attagtcaat cttgtgttat tactggtggg tatgtttatg gatgtggcgc ctgctatctt
     6541 gatttttaca ccaatatttt taccaatcgt cacaagtgta ggggttgatc ctgtacatta
     6601 cgggttgttt tcaatcatga atctgtgtgt aggctccatc acgccgccag taggtacagg
     6661 actatatgtc ggtgcaagtg taggaggcgt aaaagcagag gcaatgctga aaccgcttct
     6721 tcctttttat ggggttatct tagtcgtctt gtttttgatt acttacttcc cgcaaatcgt
     6781 catgtggcta cccaatatgc tgagttagga gaatagttat gttgaacatt acaattagag
     6841 gacatgatct ttccgaagtc caaacgatag aagatcttgc agaaaaaaca aaagaacaag
     6901 ggattcatac actgcagctc gccttaggtc tttcgtttcc tgaaacacct tctggcgcaa
     6961 atgagatcaa ttctgggatg ggaaattatg tcaaacgagt tttagaaaag caagaagtga
     7021 ccgtaggaat tttaagttgc tatatcaata tgattcatcc agatttaacg atacgacaag
     7081 agttgctgac aaagttcgag cagtatcttc gctatgcttc ttcctttggt gcgtcgatgg
     7141 ttgcttcgga aactggatgt gttttgccgg aaatccaata tacggaagaa aatttcacag
     7201 atgaagcatt tgctgaagct gtgtccgtta tccgtcgctt ggtaaaagct ggagaaaaat
     7261 accaaatgat ggtggggata gaaccaggtt tgaatcatcc ggtgtattct ttagcaaggg
     7321 tagagcagct gatccaagca gtagattcgg actatctagg aatcatttta gatccgacca
     7381 atttgatcac aagtagtgat tatcaagaac aagtccaatt ggtagaagaa gcttttgaac
     7441 gttttggtga aaagatatgt gcggttcatt tgaaagattt tcgtgtcgaa caggaaaaaa
     7501 tcgtgccggt aaatttggga gacggtgtga tcgagtacac aaaaatcaaa gaaatcatca
     7561 tgaagaatcg tccgtacctg tatgtggttc ttgaagaaac aaaagatgat gggattcgtt
     7621 atggaagaag tcttcttgaa taatgtaaag aagacgttgg ttaactaata tttagaatgg
     7681 agggggcggg gctagaagat gccacgtctc ttctttttta taagagtata taaaagcagc
     7741 tctttttgat tacaaaaaac gtggtataat aaaattgtgc ataagtgaac aaaaggaggt
     7801 gcggcacatg ctccaaaaga aaatcagcgg tgaacaagtc aatacgatgg tttctgatca
     7861 gtatgaaatc tatcatgtca aagatgtcgt cagccaaaat aaaacgatct atcatcatca
     7921 tgatttctat gagatccatg ctacattaaa gggagaagcg actttttatt tgaacggacg
     7981 acaatttacg atcaaagcag gaagtgtgct gctcatccat tccaatgatt tgcatcgtat
     8041 cgtccgccaa tcttcagatg tattcgagcg tgtctatatc tttgtcaccc cttcttttct
     8101 agaaagtcgt tccacgccat ggtctaatct atcggcttgt tttcaaccaa tgggagagaa
     8161 gaggagtcgt gtattgcaaa tagaacagga agtattgaag caaaaattat catttatcga
     8221 ccatcctcct gataaaaaaa tttacggtga agacataaaa tatgaacaag ctctggtaga
     8281 atacttgatt tttctcaatc aagttgtaca aaaagaagag agtgcgcaag aacagaatgc
     8341 tgtagttcct aatgagcgtg tggaacaaat gttacaatat atctcaaaaa atttatctca
     8401 tccgctttct ttagaggaaa tggaaaagca gttttttgtc agcaaatacc atatcacacg
     8461 agaatttaaa aagtacactg gatttacgtt tcatcagtat gtaatgaaga aaaagctgat
     8521 ctatgccaag cacttgttgc gagaataccg cagctctagt gctgtatata gtaaaagtgg
     8581 tttttcttct tatccgcatt ttctgaaagc attcaagaga gagttcggca taacgcctaa
     8641 agaatttttg aaacgtgata cacagaaaca gttggttcat tatgaacatt ttgaagatga
     8701 acaatgagaa tgatcactaa aaaataaccg cagtagctgc tgttgtttac atagaagcag
     8761 aaactgcggt tattttattt ttatctatat atttcaaaat attttgaaaa aatgacgtac
     8821 atattatatg aatcagctga atttacgaaa gtctctcggc gatagtcctg tttttgcttt
     8881 gaacaggcgg ctaaagtaag caaaattctc aatccctatt tcttcggaaa tcattcgaac
     8941 cgtaaattct gtataccgaa gaaggtcttt agcgtaagaa agttttttgc tgatcaaata
     9001 atcgattggt ggagaaccga taaatttgga aaaatctttg ctcagctgat atttattcaa
     9061 taagaattgt tgttctaatt gatcaagtgt aatcgtttct ttgaaatgtt cgtctaagta
     9121 ttgcttcagt ttgtaaacgt aggcgggaat atcctcttct tcgaaatcta gctgatactt
     9181 ttgcaaaatc agttcattca gcaactcgtg gatagctaca gaggactgga aatcggtacg
     9241 ggcattcgtg ccttgttgca tgtggatcaa ttctgtgatc aaatggtgga tcttgttttc
     9301 tgatggatcc cctgttgaat ggaaggtgtt atggccgtct tttgtgaact cctgataaaa
     9361 tgattggacg ttctgaccat tcaaatggat ccaatccatt tcccatggtt cttcgctgat
     9421 cgtttgataa cgctgatatt cttggcagtc aataaaaaat aagtctccag cttttagctg
     9481 ttgctgatta tcattatatg taagcgaacc tttgccagac aaagtaaact ttatcaagta
     9541 agaaggtaaa tgtgctcgtt cagtatagta agggattggt gctttgaagt aaccgatttc
     9601 ttggatataa aaaaataaat tcctgcttct ttgtgtaggc gtcaagatca ctctttttga
     9661 gtcttccgac catgagtggt tggctttttc ccagtaatcc atgtaaacac tcccaacttg
     9721 tcaagataat gcaattatat accaaaatag tgtgaagcaa aatataagat tctgtattat
     9781 gatagcattg ttaataaatg ataacgcttt attaataaag tgctaatcaa caataaagta
     9841 aaagaggtgg agaaatgaat ccaacatttg ttgccatcga tatcggtgca tcaagcggac
     9901 gactaatgaa aagtacgagg gatgcaaaag gttctttgat cttggaagaa gtacaccggt
     9961 tcaaaaacgg ctttcggaac aaagacggtc acgatcgctg gaagatcgat tatcttatca
    10021 aagagatttt gattggattt gaaaaattaa agcagacagg gattgatcgc ctttttgcgg
    10081 ggatcgatac atgggcagtc gattactgtc tgattgatga acaaggggat cggataaatg
    10141 atccgattgc ttatcgggac aagcgtacca atggagcagt agaacagttt tctgaaatct
    10201 attctctaga taagttgtat gagcagacag ggattcagat ccagcctttc aataccttgt
    10261 tccaattgtt tgctgaagaa aaagaaaatt tggcaaaagc acataaattg ttactgatac
    10321 ctgattattt agggtatgtc tttactggta acatggtgac ggagaaaact aatgcatcta
    10381 ctatgcagct gctgaatgca gggacaaaag aatgggaaat agaacttctt gaaaaaatcg
    10441 gtgtctcgaa ggaattattt ccaccattga cagagccggg aacgatacta ggagatctgc
    10501 aaaaagaaca attttcaaaa tatgatttgc cagatgtgac ctttatcaca gtagcaagtc
    10561 atgatacggc gtcagctgta ttaggaacgc caggtcaagg agaacgttgg ggttatatca
    10621 gcagtgggac atggtcgctt ttagggattg aaacaaagat cgccaatgtt tctcgcaatg
    10681 ctttttctga aaattatacg aatgaatggg gtgcccataa cacggtgcgt tttttgaaaa
    10741 acatcatggg gatgtggttg atccaggaag ttgcccgaat gcaagagtac gagtacagtt
    10801 atgcggaatt ggcagagctt gctgcaaaag aaaaaccgct gcagcaagtg atggaaatca
    10861 atgaccctcg atttttgaat cccccaaata tgatagaaga gattcagcaa tactgttttg
    10921 aaacagagca gccagtccct gtctcatcgg gagctatcgc tcgttgtgtg tatgataatc
    10981 ttgctctttg ttacgcacaa gagctgatca aagtagaaca cttaactgga acgaaattgg
    11041 aaaagctgca catcgtaggg ggcggatcga ataatcgtct actgaatcaa ttgacagcag
    11101 attatgccgg aatcattgta gaagcaggac ctagtgaagc cacagcaatc gggaatgttg
    11161 ccatgcaaat gctcagtaca ggtcagtttt cgaatataga acaagcaaga aatgtaatca
    11221 aacagtcgtt tccatgtgaa actttttacc cagatgaaaa taagcaaaac acgatggaat
    11281 ggtacgtgaa taaaatcaaa ggagtaatcg tatgaacacg attgaaagaa attacgaaca
    11341 agcaaaagaa aaatatgcaa ctatcggtgt agatacagat gctgtactag aaaagataca
    11401 ggacatcaaa atttctatgc attgctggca aggagatgat gtcaaaggtt tcttgacacc
    11461 ggatggcgaa ttgaccggtg gaatcatggc gacagggaat tttccaggag ccgcacgaac
    11521 acctgaagag cttagacaag atctggagaa ggcttattcg ttgataccgg gcaaacacaa
    11581 attgaacctt catgctatct atttggatac agaagaatct gtcgatctaa atgaaattga
    11641 gccgaaacat tttgaaaaat gggtagagtg ggcaaaaaaa gaagaaatcg gtcttgactt
    11701 caatccgact ttcttttcgc atccaatgat gaaagacggc tttacattgg ctcatccaga
    11761 taaagaagtt cgtgaatact ggatcgaaca cgggaaaaga agccgcaaaa tcgcggagta
    11821 ctttgggaaa gaaactgggc aaacaagtat caataacttt tgggtacctg atgggatgaa
    11881 agataatccg attgatcgtt ttaccccacg ggaacgttta atggaatcac tcgatgaaat
    11941 ttttacagaa gagttagatg aacggtatgt acaagatgca gtagaaagca aactatttgg
    12001 tttaggcgct gaagcttata cagtcggttc ccatgaattt tatatgggtt acgggttgac
    12061 tcgtgacaaa cttgtttgtc tagatgctgg acattttcat ccaacagaag tgatttccaa
    12121 taaattatct tcattagctt tattcagcaa aggaatcatg ttgcatgttt cccgtccagt
    12181 gcgttgggat tcagaccatg tggttttgat ggatgatgaa ttgcaggaaa tcgctaaaga
    12241 attagtccgt aacgaattgt tggaaaaaac aaatatcggt ctggatttct ttgatgcaac
    12301 gatcaaccgt atcgctgctt gggtcatcgg tacgagaaat acacaaaaag cactgctgaa
    12361 agctatgctg gaaccagttg agcgattgaa agaaatggag ctggcttttg actttacttc
    12421 aagaatggct tatacggaag aattgaaaga tttcccgtat gccgatgtat ggaattattt
    12481 ctgttacaaa aatcaagttc cagttggttt ggattggctg gaagaagtcc aggaatatca
    12541 aaaagacgtg ttggaattaa gaaaatagga gtgtggctat attgaaaaaa atagatattt
    12601 tacaagcgcc ttatgtgaaa gaaatgatcg aaacgacgtc gaatctttat cgcttgggat
    12661 gggatgaaag aaatggcggg aatatttctt atttactaaa agaagaagag atcacaccgt
    12721 tcttggatcc tgaaaaagtg atccgccgca tctcgatgat ttttgatgcc agccgtttag
    12781 caggatgcta ttttattgta actggttcag gaagatattt taaaaatgta gcgagcgaac
    12841 ctgccgagaa tcttggaatc gttcgagttg ctgaagatgg caaaacactg gaacttttgt
    12901 gggggctgga aaacgaatgc cttcctacaa gcgagttgcc aagtcatttc atgagtcata
    12961 tcgcccgttt agaagtagac ccagataatc ggatcgttat gcattgccat gctagtcatc
    13021 tattagcgat gagttttacg catgaattag acgaacgttc ctttagtcgt acgctatggc
    13081 aaatgtgtac ggaatgctta gttgtttttc cagaaggaat ttccatcatt ccgtggatgg
    13141 ttccgggaac aaatgagatt ggggaagcaa cagctgaaaa aatgaaagaa actcgattgg
    13201 ttttatggcc acaacatgga atctacgggt caggaaaaga catggatgaa gtctttggtt
    13261 tgatcgagac agcagaaaag gcagcagaag tctatacgta cgtcaaagcg caaggaccaa
    13321 tcttgcaaac aatcacggac gaaaacctgt ggcatttagc agatgcgttt ggtgtcactc
    13381 caaaagcagg ttatttggag gaagttcata cgaaagcagg tgtctgacat ggtgaaaaaa
    13441 gctttttgca tgaaagttta cccagaaaaa caagaagaat acaaaaaaag acatgatgaa
    13501 ctttggccag aaatgcgtac agcacttcgt gaacacgggg tcatcgacta ttcgatctat
    13561 cttgatccga gtacaagttt gttatttggt tacttagtga tcgaagatga aaagttatgg
    13621 gaaaaaatgg caaatacatc tatcaatcaa aaatggtgga agtacatgga agatatcatg
    13681 gagacaaatc cagatgcctc tccagtcagt tgggaattga tgccagtctt tgaactgtag
    13741 caacaagcaa ctgggcaacc ggttgcttgg ggttctttcg tttttattgt gaaaaaaaat
    13801 acaaattagg aggaatagaa aatgagtaat cgaatgattt taaatgaaac atcgtatttt
    13861 ggaaaaggag cgattgagtc gatcgtccct gaatttcagc agcgaggatt cggcaaagca
    13921 gctgtcatta ctgacaaagg attgatcgag cacggtatag caacaaaagt aacagaactt
    13981 ctagataaag cagcaatcgc ttatgcttta tacgacggga tcgtacctaa tccaacgatc
    14041 caaaatgtaa aagatggagt ggcttttgtt aaagaggcag aggcagactg tttgatcgca
    14101 atcggtggag gttcgccaat cgatacggca aaagccattg ggattatttt gacgaatcca
    14161 gagttttcag acgtcattag tttagaaggt gttgctgata cgaaaaatcc atgtttacct
    14221 atattagcta taccaacgac ttctggaaca gcagcagaag taacgatcaa ctacgtcatc
    14281 acagatgaag taaatcaccg aaaatttgtt tgtgttgatc ctcatgatat cccaatcgtc
    14341 gcgttcatcg acagcgatat gatgatggga atgccgaaaa aattggctgc atcaacaggt
    14401 atggatgcta tgacgcatgc tattgaaggg tttatcacaa aaggcgcttg ggaaatgact
    14461 gatatgctgc atttaaaagc aatcgaaatc atcggccatt ctttggaagt ttctgtagat
    14521 ggcgatcaaa atgggcgaga aaaaatggca ttaggacagt atattgcggg aatgggcttc
    14581 tcaaatgtcg gtttaggact ggtacacggg atggctcacc cgttaagtgc atggtacaac
    14641 attcctcatg gcgtagcgtg tgcagcactt ttgccaactg tcatgaaata taacaaagaa
    14701 tatactggag aaaagtaccg tgagatcgct cttgttctag ggattaaagg agcagctgaa
    14761 atgtctttag aagatgtaag agaagcggct tgtggtgaga tcgatcggtt gagcaaagcg
    14821 gttggaatcc cagaaaccat ttctgaatta ggtgtaaaag aagcagatat tccagcaatt
    14881 gcagaagatg ctttgcgaga tgtatgcaca ccaggaaatc caagagaaac gacagtagaa
    14941 gaaatcattg ctttatatca aagtttgatg taaaaacgaa gcggatcttc aacattaaat
    15001 tgcctgtaaa gagaaatggt agtttatatc tggatatctc ttttaaattg atggtatttc
    15061 cgctataatt tttcttataa agaaatagga gaatattata tggaaaataa aaagccttat
    15121 ggaactgtat tgatcaaagc ctctaaaata ctagactatt tagcggaaaa ccaagatgtc
    15181 tctttgcaga cgatcgcaaa gggtgtggga atgacctctt ctacagtctt gaaaatacta
    15241 gatactttgt tgatgatcgg ctacgtaaat aaaaattcag agaaaaacta tcgtttaggt
    15301 gcaaagctta ttcgctacgc gaataaaaac attgaacaaa tcgatttggt agagttgact
    15361 ttaccgtttt tagaacagct gcagcaaaag atcgatgaga ctatccactt aggggtattg
    15421 gacaacaatg agatcttgta cgtgaacaaa ctagatccaa aacatcaaac gatccgcatg
    15481 tcttcaaaaa tcggaatcac ccgaccatta tacaattctg cgatgggcaa agccgtatta
    15541 gcagaattca gtgaagagca gtacactgat tatcttgaaa agcatccgtt gatcccttac
    15601 acagaatata cgatcaccaa tgcattacgg ttgaaaaagg aaatccaaac ggtaaaagaa
    15661 acccacatcg catttgacga tgaagaaatc gaacgtgata ttttttgtat aggagcctca
    15721 atcgtgaaaa acgaacaaat cattggtgca tttagtgtaa gtatgccgaa atatcgttta
    15781 acagatgaga caaaagaaca aataatcagt gccctcaagc aaacaaaaag cgaaatcgag
    15841 caaagaattc aaaaataatg aaaaaagcgt ttgcaatttt tcttttctgc cgtataatat
    15901 gggtatagat ttccaagtta taaaataaaa tttctaaata ggaaataggt ggagaagatg
    15961 aaaaaaattg atattttatc tcgattgaaa aacgccggag tgattgcggt ggtacgcgga
    16021 aaaagcaaag aagaagcttt gaatgcttgc cacgcaatca tcaaaggtgg tctaactggt
    16081 attgagttga cttttacagt ccctcaagca gatcaagtaa tcaaagaact tttgtcattc
    16141 tacaaagacc aaccagaaat agtgattggc gcaggcacag tattagacgc tgtcacagct
    16201 cgcttggcta tcttagctgg cgcagagtat attgttagcc cttcctttga tcaagaaacg
    16261 gcagagatgt gcaatctgta tcaaattcct tacttaccag gatgtatgac tattaccgag
    16321 atcaaaacag cgctgaaaag cggggtagat atcgtcaaac tatttcctgg aagcgcttat
    16381 ggaccaagta tcatttctgc cttcaaggcg ccgatgcctc aagtcaatat catgccaact
    16441 ggcggtgtga gtctggacaa tatgaaagag tggttcgatg ctggtgtcgt aacagtcgga
    16501 gtaggtggga accttttagc acccgcagct acaggagatt ttgacaaagt aacggaagtc
    16561 gcgcaacagt atgcagcaaa aatgaaagaa atcaagaggt agctgcttat gggaaaagta
    16621 gtgacactcg gagaaatcat gttgcgattg tccacagagc agggaacccg tatcgattca
    16681 agtgaacgat tcgctgccca ttatggcgga ggggaagcaa acgtagcaat ttctttagct
    16741 aattacggac atgaagcgat ttttgctaca aaagtacctg ataacgcatt gggaaaagca
    16801 gtcaaaaagc acttgcaatg ttatggagta gatacgaaac aggtattatc tggtggatca
    16861 agactaggta cttattatat ggaaactggt agtggcgaac gcgccgcctc agtgatctat
    16921 gatcgtgccg gatcaagttt tgctgaaatg gatcatttgg aatggtcgct agaggagtta
    16981 ttccaaggaa tagatatatt tcatgtttca ggaatcaccc cagctttgtc taaaaaatgg
    17041 caaaaaatga cgaaacaggt gatagaagct gcaaaaaaag ctggctgcct gatcagcttc
    17101 gatatcaact atcgtggaaa attatggaca cagcaagaag ccagcgaagt aatccatcag
    17161 cttttacctt tagtagatat ctgctcagct ggaaaaatgg atgctcttta tttgttaggt
    17221 atcgagaaag cgccagaagc agaagaacaa cctctaattt attattatca aaaaatgcag
    17281 gaaaagttcc cgaatattca ggtgttttat tcgaccaagc gtaccgttca ttcagcaagt
    17341 gaaaatgaat tgaaggggac attgtggatg aaccatgaat atgtggaatc ccaagtccat
    17401 agaatcaccc caatcgtgga tcgagtaggt ggcggagatg cttttgcagg tggcgtgctc
    17461 catgggattt tatccaatat gtctccgcaa gaaatcattg attttgctac ggcagcttct
    17521 gccttgaagc atacgatcca tggggactgc aaccaattta gtcaagaaga agtcgcacaa
    17581 tttttagcat gcggctctgg aaaaatcatt cgatagaagg agtagaagaa aatgcaagaa
    17641 atggaaacac gttatacaca cagtccagaa gatatccgtc attattcaac ggaacaattg
    17701 agaaaagaat ttttagtaga aaaggtattt gaaccaggga aaatcagttt gacttatacg
    17761 cacaacgatc gaatgatctt tggtggagtg acacctactg aaaaagaatt agaaatcaca
    17821 ttagacaaag aactaggtgt tgattacttc ttagagcgtc gtgaactagg tgtgatcaat
    17881 atcggtggac caggattcat cgagatcgat gggcaaaaag aagaaatgaa aaaacaagac
    17941 ggatactata tcggaaaaga aacacgacat gttgtatttt cttctcaaga tgctgaaaac
    18001 ccagctaaat tctatattag ttctgttcca gctcatcata aatatccaaa tgtaaaaatc
    18061 agtatcgacc aagtaaaacc aatggagact ggtgaaggcc tgacactgaa tgaacgtaaa
    18121 atctaccaat acatccatcc aaatgtatgc gaaagctgtc aattacaaat gggttatacg
    18181 attttagaac caggaagcgc ttggaatacg atgccttgtc atacacatga acgtcgtatg
    18241 gaagcttatg tctactttga ttatgccaat gaagatacac gtgtgttcca catgatgggc
    18301 aaaccagatg aaacaaaaca tttagttgta gacaatgaac aagccattat ttcgccaagc
    18361 tggtcgatcc attcaggtgt aggtacaagc aattactcat tcatctgggc aatgtgcgga
    18421 gaaaatatca cctatacaga catggatatg gtgcaaatgg atcaattaaa ataagcataa
    18481 gggagagaaa atgatggaat ttagtatgga tatgtttcgt ttagatggaa aagtagcatt
    18541 agtcaccggt gctgtctatg gtattggatt tgagattgca cgctctttag ctacagcagg
    18601 agcaaaaatc gtatttaata acttgaatca agaatcagtt gatgaaggta tagcgcatta
    18661 taaagaagca ggcatcgatg caaaaggtta tgtctgcgat gtgaccgatg aagaagcggt
    18721 tcaagcgatg gttcaacaga tcaaagaaga tgtaggttca gttgatattc tagtcaacaa
    18781 tgcagggatc atcaaacgga caccgatgat cgaaatggat gcggcagact tccgccaagt
    18841 aatcgacgtg gatttaaatg ccccattcat cgtttcaaaa gcagttattc cggatatgat
    18901 cgaaaaaggc ggcggtaaaa tcatcaatat ctgctccatg atgagtgaat taggacgcga
    18961 aacggtcagt gcttatgctg cggcaaaagg cggattaaaa atgctgacga aaaacatcgc
    19021 ttctgaatac ggtcaataca atatccaatg taatggaatt ggaccaggtt atattgctac
    19081 accgcaaact gctccattac gcgaaacaca agaaaacggt gaacgtcatc cattcgatca
    19141 atttatcgtt ggacgtacgc cagcagcacg ctggggcgag ccaatcgatt tggctggacc
    19201 atctgtcttc ttggcttcta gcgcatctga tttcgttaac ggccatgtct tgtatgtgga
    19261 cggaggcata cttgcctata tcggaaaaca accttaaaag agctttagta cactgtttag
    19321 tggagaggtt gtgaaatcag cggattgaac gaataccgaa ggacagctga ttaaacatca
    19381 tttattcgaa aaaagaggct gggacagaag catttaactc caagaaataa gaaggaattc
    19441 acgaaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga gttgctttta
    19501 cttccgccgt ttgtacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
    19561 gctcttttct gtgcgtcttc catacttcac taagtcgatc cctcttgctt cgcaccaatc
    19621 cttcactggt ccggaaaaga tcagagattg ttggtgtagg gccgcggtat tcgttgcgcc
    19681 gaccatagta taaagcagac gaagctcttc ttgccattgc ttcatcagta taatagtttc
    19741 ttctacgccg tgattcatca gatgcgtcag tacggttcct gaagtaccca cggcatttgc
    19801 gccgagacaa agggctttaa atatgtcata ggcattgcgg atacctccgg aggctaggat
    19861 ttccatagaa gtgtctgctt catttgcttc aagcaatgaa gagactgtcg attgccccca
    19921 gtcagctaaa taactcagtt cccgcttgga gcgacgggcg ttttcgatct gggtgaagct
    19981 tgttccgctt cgaccgctga tatcaactgt atgaactcct aaagcagcta gatcattgag
    20041 cgtttctctt gtcatgccaa aaccgacttc ttttacgatg agggggacgt caatggctgt
    20101 ttgagtttct tgaataagta ctttccaatt ggtaaaatca cggtctccct caggcataac
    20161 caactcttga ggtgcattca aatgaatctg taaggcatct gcgtgaaaca atcggatagc
    20221 ttctttggct ctttcgacag aagtccctgc accgatgttg gcaatgattt tcccgtgagg
    20281 atattcttgt cgcataatcg tataagtatc ggctaaagaa gggactttca aggcagcgct
    20341 gactgaacca gtggcaatca tcaaatctgc ttctcttgcg acgattgcaa gatcttggtt
    20401 gatttttttt gttttttcgc ttccgccagt catcgcattg atataaaatg ggctgcttag
    20461 tgtaaaccca actgcctgtg tacttagatc gacatcagaa actgctattt gcggcaaagg
    20521 gttatggatg atccgtacga agtcaaattc gtttttttgt ttatcatgaa aggctttagc
    20581 tagtgaaaca tgttcgtctt ttcgattcat cggttttcct ttctttgatc gctatagaca
    20641 tgtaacggca gtggagtgat ttctgctttt tcccatgcac tcattaaagg aagaatgcca
    20701 gatttctggt caacaattac gattccgcaa tcgcccccac ctgcaccaga agattttgcg
    20761 gctccttcat actgttcagc taaattacac aatttgttca aagcaggcgt ttcgatgacg
    20821 accccagtga ttgcagataa atcatgcagt aattgtcggt tttttcgaat catctgttgg
    20881 atcaacgtta cattattttc tttaaaccct ttgatcattt cgttgacaca ttctgtacta
    20941 ttttttaaga aaagctgata agccaccatt ttatcttctc tcgaacggtg aacttgatcg
    21001 accaaatcag aagtagaggc agggctaccc gtccaaccaa tcaataaacg taaatcttca
    21061 ggagcaatca atggctcaat ggatagacct ggccaatcca gggcaagtaa ctcactgata
    21121 gaatgctgag tttcttgttc ttggagccaa ggatggtcga aggttgagaa agcgatccag
    21181 ccaccataac agctagcagc gatgtcgccg caggaaccat tatcttgaac tgctaaattg
    21241 gctagtgctg caatcttgaa aatctccaac tgagataaat tcaacgcgta aaaaacattc
    21301 aaggctttga ccgttgcaac ggtcacggca ccgctagaac ccaatccata tttccggcca
    21361 tttgaactgt ctaactcact tgttactttc agatcataaa aggataaaag aatgtttttt
    21421 tcttgtgcat acttttcagt caagcgaata gcagcaagga tataatgaaa aggattttcc
    21481 cgaatatcta aaactaattc tccgttgcgt cttgtccaac gtacaggcat cccactatat
    21541 tgtgcagatt ggatacttcc gacttttcgt gcagattcta cagtgactgt cacgaattga
    21601 tcgactgcag cgataactgc tggatggcct gtttcaacaa ctgcatattc tccggcaata
    21661 tagagtttgc ctggtgcaga tacttcaatc ataaaaatct ccctttattc aataatcgca
    21721 attcctggac cagcatgtgc agtaattact tgttctttgc taaataattt ggtaaatgtt
    21781 tcttgtacct cagataaatg gctgttttcc actaaaactt tgacattagg gccagcatcc
    21841 atcgtaaagt agcatggaat gccttgcttt ctcaattgtc gcaccgcatc catcgctttt
    21901 aagctgtcag gagaccagta agtgaatgga ggctgggcag ctaaagtcgt tccatgcatt
    21961 tttagtccgt ttctttccat cgtttctccg agaagttgga aatcttttgt tttgattgct
    22021 tgtttcaatt gatatagatc cccttcaacg gaatctaacc agccttgata aaaactggat
    22081 gtttcgactg tccgacgcat cccatttctg ctggacactt cttttttctg gtcgttgatc
    22141 aaaacgaaaa ccattgctaa atcgtcttcg aaagaatcgg aagggactgg cttagcgtaa
    22201 gaacttaagt cgtcatggcc tttttcccat tcgacgaaac caccgaaaat acttcggcaa
    22261 gctgaaccag acccgcgtcg agcaaatctt gaaagagaga gatcatctaa tccaagctta
    22321 agagcagtat tgcaagcgcc tgctaaagca gctagcccgc tggcagagga ggctaatcca
    22381 gctgcggtag gaacgaaatt ctgactgatt actgaagctt ttagtgagag gccggcttct
    22441 ttacgaaaaa ggtcaagaaa ctgactgact ttttttgttg ctttttcgtc ttgcaaggtg
    22501 ccatctagat aaaattcatc taccatatag ctatcagaaa agatgacttc tgtctctgta
    22561 taaaatgcat ccaatgttaa cgaaaggctg ttgttcattg gaaggatgag ttcttcattt
    22621 ttcttacccc aatatttgat tagagcaatg ttggtatatg cgcgtgcttt gcctttaaac
    22681 ataggtatgt actcctaatc cttgtatcca agtttcagca gcacccgcat cttcaagtgc
    22741 ttgactgatt tcttgggctt ttgtttttgt ttgtgctaat gcaatcatac agccaccgcg
    22801 tccaccgcca gttaatttgg cacccaatgc cccggtatct tttgctgttt ggatcaatag
    22861 attaagaaaa tcattgctga ttgtcagctt ttccagaagt gattgtgatt catccattgt
    22921 ctgtgccaac gtttctggtg aattttcaat gattgcttgt tttgcttgct tcgtcaagta
    22981 tcctaatttt tggatcattt gtccagtttc atgaggctgg gtttcaaaga ggtgagcgac
    23041 atctttgacg gcttctcttg tttgtccttt gattcctgta tcagcgacaa tcaaaaaagc
    23101 atcgatgttc aaagaaaagt agtcgaaagg atgccctttt gtaaaataaa tcggctgcaa
    23161 gctgctagta gcggctgcat cgattccact aggattaccg tgggcgattt tttccgaaag
    23221 ctggacattt tctaatagta tttcacgaga caaaggaaat gctaagtaat cataaaaagc
    23281 acgagtgact gctgtggcga ctgcagcgct tgatcccatt ccacgttcag caggaatcgt
    23341 gctttcaatg gtcagttgca agtttttttg gatgtcatgc tgttttttta agtgaatgaa
    23401 taattttttg atatttttta atgcatgagg agcttcggct aaatttcctg aatagtagga
    23461 agaaaccagt tgatcgtcca ttgcatccag ctcttcaagg aatgcggtga cttttgttgc
    23521 atagaaagga aaggcaatcg ctggttctcc ataaacaacc gcgtgctcgc ccatcaatat
    23581 gatctttccg cttgattctc cttggccata gtttgccata atctaactcc ttctcacgta
    23641 aaagtgattt ttaagcaaaa aataaagcgc ccaaaacgag ttgcgctgct tcacgaattt
    23701 ttttgtccac taagggtctc aaaaaacatt cctcctttat tttactaaaa ccatctcaga
    23761 agtaaaacta agaatacgtt ttctaaagaa aaaacacttt tttcttaaga attttttgac
    23821 tttttttctt ctttgactag aaaaaaatac aattgacact actagatatt gggggtatat
    23881 ttaaaaaggt tggagttaaa cacaatattt agtgatgaag tgagctggag gtaaatgagc
    23941 gtgatcgttt tagcaggtac aattggtgcc ggaaaatcaa gtttaacaga aatgatggct
    24001 gaacactttg atagtcaggc attttatgaa tcgattgatg ataatgaagt cttgccctta
    24061 ttttatgcaa accctgaaca gtatgctttt ttgctgcaaa tttatttttt gaataaacgt
    24121 tttgcaagca tcaaacaagc aatgaaagat gacaataatg tattggaccg ttcgatttat
    24181 gaagattcac tgcttttcca tctaaatgca gatctaggaa gagcaacaga aacagaagtt
    24241 cgtgtgtatg atgaactgct ggaaaacatg atggaagaac ttccctatgc tgcccataaa
    24301 aaacatccgg atttattagt tcatattcgt gtatcttttg atacgatgtt ggagcgtatc
    24361 gaaaaacgtg gtcgttcgta tgaacaattg tcctttgatc caagtctata cgattattac
    24421 aaagaattga acagacgtta cgatcaatgg tatgaagaat acaaagaaag tccaaaaatc
    24481 caaatcgatg gagaccgcta taattttgtg gaagatcctc aagcaaaaga agaagtcttg
    24541 aaaatgatcg aagaaaaatt agcagagatt cgccaaacca aagtagccat ttgatttaca
    24601 gttgctcaaa taaagggtaa acctttattt gagcgacttt tttctttata ctggaaacaa
    24661 atgtcaggag gatagagtat gcgggaaaaa cactatttta atcaagaccg taatttttgg
    24721 atcaatgtac agcaaaacga tgtgctagct gttaaaaact taaaagaaga atatggcatt
    24781 agtgacgaga tgctgactta ttctctagat aaaaatgaac gggcgcgcgt agagtacgat
    24841 tcttttgatg aagcgttact gcttgtatca aatgttccgc accagcaaaa agtcgataat
    24901 cattatgaaa ctagtcctat cgcttttatt ttaaaagaag acggtttgtt tacatttacc
    24961 acgccgaata ccgagtacgt gattcgacta attcgatcgc ttttggaacg aatgccagac
    25021 atgagtgtat acagtttatt gtttcgtaca ttgtttttga tttctgattc cttttttcct
    25081 ttaatcgagg aagtgaatag tgaacgtcag cggcttaatc tgaaattaag agaaaaaaca
    25141 acgaacaaga atctgctgca actttcagat ttagagatcg gtttggttta cttagttaca
    25201 ggaacaaaac aaaatgtagt acttttagaa caaatcaagg cattggctat ttatcgcaag
    25261 ttgtcagaaa aggaaaaaga agagttggac gatgcgctga tcgaagcacg acaagcagtt
    25321 gaaatgacga acttagcttc gcagatttta gaccagctct ctggcactta taataatttg
    25381 ctgaataaca acttgaatga cacgatgaaa tttttgacgg tctggtcgct gatcttgacc
    25441 gttccaacta ttgtgacagg attcttcgga atgaatctcc agttaccctt tacccattcc
    25501 gttttcggat ggggaatcgc cttgatcatc agtttggtct tatctatttg gatgcttatt
    25561 gcactgtggc ggcggattcg ttgatctagt ctttgatctg caaaccaagg gcttgggcga
    25621 tcacaagtgc ttgctcttgg ttgacgatta gtcccgccaa agaatcttga gataaagcga
    25681 ttgctgtgaa gaaattagtc gtaaagtcta atcccttcag agaagttcga aaccagttcg
    25741 aaccggtcaa ctgattgtca gacaaaaaaa ggtttttcca agtgacctca taaaattcgc
    25801 tgtcttctaa ttgacaatga tcgaaggaga cagctttcaa gtttgtacta ctaaaggaag
    25861 ccatcgaagc aatacaatca gtgaaagtac agtctcgaag ataactttcg gcaaaattcg
    25921 tacctgttaa tttacattga tgaaacacga cttggtggaa actgccacca atccactcca
    25981 gattggaaaa atcacatttt tcaaaaatga cattactgca ttcaaaatga cgtaaaactg
    26041 ttttttgcat cgtaagtttt tggatatgac tctctcttaa taccatattt ttggcatcga
    26101 gatagctttg gtcacccccg ttgatcagaa gaccagagaa gatctcctca tcttctaaat
    26161 aaaactctcc ctttgtaaga agaggaagag aaggtggaat aggttcgatt tttttcatgt
    26221 gggttcacct cgtttttctt tagtatagca aagaagcggg aatcactgct agaaagtctg
    26281 ctaatctaat cattttcaga aaaggaagac aaactgtatg aaaaacattg acaaaaagat
    26341 tagtttttgc tatactatga tggttgagaa ataaaagcag aagcacccgc ttctcgcctt
    26401 agttgataat gtcactgggc tagatagttt tcactggttc tattaggtga agtacgcgcg
    26461 ggttctgtaa gagaactcgt ttttttattg ccgtttattc ggtaagcttt tctttctctc
    26521 ataatatttg gaggtgaatg accatagcaa aggatatgat ggttaacgac ggcattcgtg
    26581 cacgagagtt acgattgatc ggttcagatg gtgagcaatt aggagttaag acaaaagcag
    26641 aagcattgaa tattgcagaa caagcaaact tggatcttgt gttagttgcc cctggtgcga
    26701 aaccaccagt tgcgcgaatc atggactacg gtaaataccg tttcgaacaa caaaagaaag
    26761 accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa agaagttcgc ttaagtccta
    26821 cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc acgtaagttc ttggaaaagg
    26881 gcgataaagt gaaagcttct atccgtttca aaggccgtgc cattacccat aaagaaattg
    26941 gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga tattgcaaca gtggaacaaa
    27001 aagcgaaaat ggacggacgc agcatgttct tgacgctggc accgaaaaac gacagtaagt
    27061 aatttgaatg actaactggc tgttggacag ccgatagtcg gataacgaaa gattttgagg
    27121 aggaaatata gtcatgccaa aacaaaaaac acaccgcgga tcagcaaaac gtttcaaacg
    27181 tactggtaaa ggcggattga aacgcttccg cgcgtttaca agtcaccgtt tccacggcaa
    27241 aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg gtttctagtg gcgacttcaa
    27301 acgtattcgc caacaactag caaaaatgaa ataagaagta caaataacag aatctgaaga
    27361 tttattttta ttaggaggaa ttatacatgg cacgtgttaa aggtggaaca gtaactcgta
    27421 aacgtcgtaa aagagtgcta aaattagcaa aaggttacta cggttcaaaa cacactttat
    27481 ttaaaacagc aaaagaacaa gtaatgaatt catacaacta cgcatatcgc gatcgtcgtc
    27541 aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat caacgcagca gctcgtatga
    27601 atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt tgctgaaatc gacatcaacc
    27661 gtaaaatgtt agctgacttg gctgttaacg atgcagcagc atttactgca ttggctgacc
    27721 aagcaaaaga agctttggct aaataagcta aacaattata aagaggaaag aagataacga
    27781 aaagttatgt tctttcctct ttttttattt acaatcctcc atcaaatgaa acaggacaat
    27841 aacgtataag tctaaaatag cataattaag gttttttaat tgaaaactga acatttatgg
    27901 tcacccttta atgaaagcgc taactacata agttagaatg agtttgactg atatattagt
    27961 ttgcttatca gaaaaacaaa atggacggag gaataacatg agtgatatta ttattggtat
    28021 tttgttagtt atctcattct ttttcatggt ctggtactgt gtaaaaggct acaacttgat
    28081 ggtcggcttt gccatcatgg ctactgtatg gatgggatta gcgctagtcg gaaacacgtt
    28141 ttcaccaaac ccggcgatgg aagggcaagg agtaattgat gtgttgacgc atatctatac
    28201 gacggggcca gctgagtacg ccaaatcgat cttggtaaat gtcttctttg gtgccttttt
    28261 cgggcgtgtt ctagtagata gcggaatcgc agctaccctg attcgaaagg tagttgaact
    28321 tggaggggac aagccaagaa tcacgatgag cttactatgt attgtaaccg ctgttatatt
    28381 catgtcgatg acaggaatcg gaccagtgat ttctattgca gttattgtgt tgccaatctt
    28441 gatgtcatta ggtatttcag taccggttgc actgttctca tttatgggct cgattatggc
    28501 tggaatcttt gctaatatcg tcaatttcaa acaatatcaa acgatttatg ctggtttcaa
    28561 tccagcggct gaaagctata cttataatga ctatttccaa attggtatga ttgggatgat
    28621 tgtaagctta gttgtggtac taacggtcgc taatatatca atgaacaaaa agaaacgcta
    28681 tgcaatggca gcaaacgtac cagctgaagg tggcgacgca ccaatgatct catggttagc
    28741 cgtattatta ccagttttag gggtcgttct tttagatctt ccaatcatct taggttttat
    28801 tctagcaggt atctgggcat tgctgtttac tggaaaattg cgcggaggtt ataaagaaat
    28861 ctgtcgtcag tttgcgaaat tatttacaga tggcgcagta gatgttgcac caatggttgg
    28921 tttcttgatg accttagcga tgttcaataa ttcagctgcg tatgcttctc cttatttttc
    28981 agctattttt ggagattgga taccgcaatc accattagtc ttggcaatcg tatttgctat
    29041 attgacacca ctaggatttt tccgcggtcc aatgaatcta gtcggttcag gctcagctat
    29101 tttagctgtc gtattagctg ttaatccaac gatgtcgcca gcctttttgt tcccgttatt
    29161 tgcaatcaca acgattgctc ctcagcattt agatattaca caatcttggg ttgcttgggg
    29221 attaggttat acaaaagtaa cttctcgaga atatatgaag aaatccatcc caactggttg
    29281 gatcatcgga gcgatcttat gcctgatcac gttcttattg tacgggaacg cctagtaaac
    29341 aaaacgtcga taaaaacgat tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga
    29401 atgggaattg atgttggcgg tacacatacg aaagcagtcg caatcgataa tgcgacgcac
    29461 gaaatcatcg gaaaatcttc agtgaaaaca acccacgatg atgtccgtgg agtagccgca
    29521 ggtgtcgttc agtcttttca aaattgtttg cgggaaaata atatcagtcc agaagatgtt
    29581 gtgtttgttg cacattctac cacacaagcg actaatgcac tgattgaagg agacgtagca
    29641 aaagtcggcg taatcgggat ggcaaaaggt ggattagaag gttttttagc aaaacgacaa
    29701 actagattga acgatatcga tttagggaat aagaaaaaaa tagagattgt caatgcattc
    29761 ttacctgtca aacacttaaa tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga
    29821 gaaagagcgg aggtcttggt ttcttctatg gcatttggag tagataatgg ggaaccagaa
    29881 cgagttgttt atgaagcagc atcggtaaaa tcgatcccta caacgatggc ttctgacatc
    29941 acaaaattat atggattgac aagaagaact cggacagcag caatcaatgc atctatttta
    30001 ccaaaaatgc tagatacggc aacctctaca gaagattccg tacgagaagc aggggtcaat
    30061 gtttctttga tgattatgcg cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa
    30121 cgacctgttt taaccatgct ttccggtcca gcagcatcag tcatgggctc tttgatgtac
    30181 ttgagagcgt ctaatggtgt ttattttgaa gtaggcggaa caacaaccaa tatcggggtc
    30241 attaaaaatg gacgtccagc aatcgattat tcaattgtag gtggccaccc aacatatatc
    30301 tcttcattag atgttcgggt tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag
    30361 tcaggaatca tcgatgtcgg tccacgttct gcacatattg ccggtctaga ttatgcagta
    30421 tttacggaga cagaaaaaat caaaggtcct aaggttgaat ttttctctcc taaagaagga
    30481 gaccctgctg attacgtcaa ggtagtgatg gaagacggag aagaagtgac tattacaaat
    30541 acatgtgcag ccaatgtcct tggattagtg caagaagaac atttttctta tggaaatgtc
    30601 ccatctgcac gaaaagccat ccaagcatta gctgattatt gtcatacgac ggttgaagac
    30661 atcgcagaac agatcatgga aaaatcctat gctaagattg aaccggtgat tttagaattg
    30721 gcagacaagt atcacttgga gaaagatcag atttctcttg taggtgtagg gggcggcgct
    30781 gcctcattga ttacttactt tagtaataaa atgggtgtga aatattcgat tcctgaaaac
    30841 gccgaagtga tttcttctat tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt
    30901 atcatccctt cgccaagtaa agaagatata cgatcgttga aaaatgaagc gatgaataaa
    30961 gcgattgaat caggcgctac accagaatcg atcgaagtcc acgtagaaat cgatccacag
    31021 acttcaaaag taacggctat cgctactggt tctacggaag tgaaagcaac cgatctaacc
    31081 aaggagatca caacagaaga agcactagag cttgctgcag aggatatgcg attaaataaa
    31141 aatgaagttt gcttattgga aaatacacct ttcttttatg tatgcggcga acagaatcgt
    31201 tcaaaaaatg ctggaagtct tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt
    31261 ggacatgctt catgtatgaa aacaactgca gccaattata tgacagctgt cgaacaactt
    31321 tgggaagata tggcagttta tcaaactgaa ttgattgccc gaccagaatt ttatctctgc
    31381 ttaggagcaa gagtctctga ttttacagcg acggatctgg aacaactaca gcttttgatg
    31441 gatctggaag tatctactat ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc
    31501 aaacaaacat aagttagaag agttactaaa cgaattacaa cagatccccg aagaaatttg
    31561 gggattctac cagtttcggc gagatttatt ttggaaaaag attcctttgt ccaagcagaa
    31621 aattcttata caacaatcaa tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa
    31681 gtaccctttt gccgatgtag gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg
    31741 tgaatcagag caaataaacg aacgaatcac ttttgcaact tatgcagaag atgaagggat
    31801 tcgattgatg acggagccgt tagaaaaatt aaaatgttca ggactagctt cgatctctaa
    31861 agaaacagcg caagctttga ttattggaca tgaactattt caccacatcg aagcttctgt
    31921 taagggaatt tatacgcaaa gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca
    31981 tcaatcaaac attcgtgcat tgagcgagat agcagcgatg agtttttcaa aagagatgaa
    32041 tcaaagcgat ttttcgcctt atgtattaga ggcagtctta ctttggccat ataatgaaat
    32101 tcgtagccaa gggatcttag aagaaataaa aaaaatagaa aaaagatgta cggagtatga
    32161 tttagattac aaataaagag actgtgacaa aagtcgtgtc acagtccttt ttttccgaat
    32221 aaacgatgtt ctcctgtcaa ctcctcggtc acaagtcaaa atattcaaca aacatgagaa
    32281 gctgtttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttg
    32341 gaaaacggtg ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacataa
    32401 gaagctattt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    32461 tcaataaacg gtgtgcaatc agctgaccct cggtattagc tcaaaaaaaa cggcaaaata
    32521 tgaggaacta ttttgccgtt tctttctcta atactcaggt caaaacgctg attccaaaac
    32581 ctcttatttg atgggtctgt cctgaatatc ttcaaaataa tcggggtaat tatgaatcac
    32641 gtattcttgc tcctcgataa tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc
    32701 aaagttatga tcaatcattg attgaagcat tgtctgatgt tcatctaata ctcgacccca
    32761 gtctaattct gaaatcgtca aacgtaacca tctgaaacgg ttcaagtgcg tactataact
    32821 ttctagccaa tcccaaattt ctcgttttcc agcaatttca taacaagttc ggtgaaaatc
    32881 agtatccaaa tcaaaaaaag cttttttatt ttgagcgatt actgcctgat cggtttttcc
    32941 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
    33001 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
    33061 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
    33121 ccaggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
    33181 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
    33241 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
    33301 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttgtatcggt
    33361 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
    33421 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
    33481 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
    33541 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
    33601 agcgtacgct tggaataact gatctaactc cggattgact ttgctcagct tcactaatcg
    33661 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
    33721 ttcagttccc acaatttcgt agctaaaatc caaccgtgtc cccgtatact tttggatagt
    33781 tggatggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
    33841 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
    33901 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
    33961 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
    34021 tcatgcctcg ttttccattg attttttaca aaaataattc atccatttcg gaagtgcttc
    34081 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
    34141 atgaagataa atagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
    34201 tgtgatcatc ccagctaaag tgtcacccat gccgcccgtt gccatagcag cagaacctaa
    34261 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
    34321 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
    34381 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
    34441 gggtagcaaa agatgattag cagcaagtaa acttaaagca gagccatcaa tcaccagcca
    34501 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
    34561 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
    34621 agtatccgtc caaactacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
    34681 tttttcatca gttactacag tcgtgagacc cgctccgctg tgtacggcag ctaaagcact
    34741 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
    34801 tcctttatgt gaaatttttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
    34861 cagcatctaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
    34921 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
    34981 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
    35041 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
    35101 gcgcgatgac tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
    35161 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
    35221 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
    35281 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
    35341 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
    35401 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
    35461 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
    35521 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
    35581 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
    35641 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
    35701 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
    35761 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
    35821 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
    35881 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
    35941 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
    36001 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
    36061 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
    36121 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
    36181 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
    36241 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
    36301 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
    36361 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
    36421 tttggcgaaa taattaatta aaaagaggat caagaattgc aaattatttt tgcagtcttg
    36481 atcctttttt tactttctac tgtggtattc atgataaaca tgatatagat tcaattcata
    36541 ttctttctta gcgttcgtga cgactgtatc caaaatcaac cctgttacta atgaaagaag
    36601 tgctaaagtc atcaatccag tacttaaaat cgcagaaggg atgcgtgtaa taaagcttgt
    36661 cagcatatat tcgcgaatca caggaatccc cgtgaccaat ccaaataaaa agaagaagat
    36721 ggtccaaatc ccaaagaaaa gcaaaggctt ataatcttta aacattttaa taatcatcat
    36781 gatcactttg aatccgtcag agaatgtatt caattttgat tcgctgcctt caggacgatc
    36841 acgataatcg attgggattt caacgaactt gaatttttta tctaaagcat ggatcgtcaa
    36901 ttccgtttcg atttgaaaac cagaactcat aataggaaaa cttttgacaa agatacgatt
    36961 gaatcctcga tagccggtca tgacatcaag aatttttgtt ttatacattc tagtaattgt
    37021 atttctaaca aggttatttc caaaatcgtg gaaaggtctc ttattctcat caaagtaggt
    37081 tccatttgat aggcgatcac caatgaccat atctgcttca ccagaacgta atttctcgag
    37141 caaaccatgt acagcttctg caggataagt atcatcgcca tctaccatta aataatagtc
    37201 ggcatcgata tcaaagaaca tttggcggat cacatttcct tttccttgac gaggttcttt
    37261 ttttacgatt gctccacctt gaacggccaa ttcgtatgtt cgatcggtcg aattattatc
    37321 atagacataa atatctgctt ctggtaattc tcgtttaaag tctgcaatga ctgttgaaat
    37381 tgttgcttcc tcattgtaac aaggaatcag aacagcaatt ttttcttgtt gttccatttt
    37441 tcactctcct taactataga aaacaaaagc taaattaaaa atacctcata atcaaaggaa
    37501 atgctagttt ttcccgctaa atatcattta tttaaaaaga aaatactgga atatatgttt
    37561 ggaaagctct atctaaaaaa ccacccacta caaaagaagg acatgatacc tctcttttga
    37621 atagtgggtg cgtattcgtt ctctcacttt tttgacgtat taatctgttg cacctttttt
    37681 tcttgaaatt tctaatacgg ttcctgtatg agcatctgct aaaaattcat aataaacagt
    37741 ttcgccatct tctattcgag aaagacctcc ccgataagca ctcgaccgaa cggcgaattt
    37801 cctaataggt tccttttcaa aagagatcca agaaccttcg attgcacctt cttttagaaa
    37861 agatgacttg atattttcaa gtacttggtc tgctgacaat acttttttgc gatgatacca
    37921 aatcgttgaa gctaccccgc tcaataatcc tagaccgatt ccaactgcta acccgccttt
    37981 aaaatagctt agttcgcttt cttcatacat gtggacatac ctccttcttc tcacttgtta
    38041 ctccttgtgc ttattttacc acactctcat attttctttg aaataaataa ccgctaccag
    38101 aaaaaataag ctataataaa tacgatcggt ctgcagaaaa aatcatattt tatccgcaga
    38161 tcaatataag tctgagagga gtttagtctg aaatttagac gaatagcata tggaagaaaa
    38221 aacatttcaa cgaatcaaag agttaacaga attacaagga acaagcggtt ttgaacaaga
    38281 tgttcgcgct tatatggaaa gtcatatgga accgctggta gatgagttac agttagatgg
    38341 tttgggcggc atttttggtc tgcgccatca tgaagaagcg gatgcacccc gtgtaatggt
    38401 cgcagcacat atggatgaag tcggattcat gctgacacaa atccaagaaa acgggctttt
    38461 taaagtcgta cctctaggcg gctggaatcc ttacgttgtt tctgctcagc gttttacact
    38521 taaaacctct actggtaaga attatccatg tatttcttct tccgttcccc cacatttatt
    38581 gcgaggaaca agcggacaaa aatcagtaga agtaaccgat attttgttcg atgctgggtt
    38641 tgaatcaaga gaagaggcaa tgagttacgg tgtccttcca ggagacacaa tcgttcctta
    38701 cgctgaaacg atcaaaacag ccaacggaaa gaatattatc agtaaatcat gggacaaccg
    38761 ctatggctgt acaatggtat tagaagcttt agaagcgttg caaaatgaaa cattaggtca
    38821 tacattgatt gcaggcgcga atgtccagga agaagtaggc ttgcgtggtt ctaaagcttc
    38881 tgtcaataaa ttcaagccag atctgttctt tgcagtagac tgctctgcag cagatgatac
    38941 agtgacgaaa aatggcacat tcggacattt aggagaaggt acattgatgc gtatccaaga
    39001 tccaggtttg atcatgcttc ctcgtttgcg cgagtacttg ctggatatcg ctgaaacaaa
    39061 caatatccct tatcaatact ttgtttcaaa aggcggtaca gatgcaggtg ctgctcatac
    39121 acaaaacgaa ggaatcccaa gtacagtgat tggcgtagtg ggacgttata tccatacgca
    39181 tcaaacgatg ttcagtattc gcgattttga agctgcacga gaaatgttga ttcaaacact
    39241 gaaaggtttg gataaatcaa cagtgaatac gatcgtatac ggaaaataat gcgatagtga
    39301 aactataaaa tctggcaaaa gattgctttt tgccagattt tttttataaa aagaggatca
    39361 atgtttgtta gttcgaaaga aaggtgtata atttaattga aaaatacata aacagaaagt
    39421 agaggacaaa gaatgattat cccaaatagt ttagaagaat tagcaggcta cgtagaaact
    39481 ggaaagagtg tatttttctt tactgccggc tggtgcggag actgccgttt tatcaaaccg
    39541 caaatgcctg aaattgaaaa tgacttttct gattggcaat ttattgaagt agaccgtgac
    39601 aagtacatag atgtagccgc tgaatggaac atttttggta tcccaagttt tgtcgtgatc
    39661 caagacggaa aagaattagg tcgtctagtg aataaagatc gtaaaacaaa acaggaaatc
    39721 gaatcgttcc tagaaagtgt aacaaaaggc tgacatctgt taggaggggt caagatgaat
    39781 caagaatata agaatatttt agtaggtatc gacggtagtg aacaagcact tgctgctttc
    39841 aaaaaagcgg tagaagtggc tcgccgtaat aatgggaaag tatatgtagc aaacgtaatc
    39901 gatcagcaat tttataactt tatgggctat gcacctattg atcaaaatct agtcgatcag
    39961 caaactgaaa cagccaagga aatgatcgaa gaatgtaaag aatatgggaa atctgttgat
    40021 tacacaaata tagaaggaat tattgcatat ggttctgttc gtgaatccat ggcagtcaaa
    40081 ttgccaaaaa aatatgagat cgatctaatc atggttgggc agtcaggtct aaatgcagtc
    40141 gaacgcttta tgacaggaag tgtagccagc tacgtgatac gtcattcacc ttgtgatgtc
    40201 ttgatcgttt ctgacgaaga agaacagcca agtaattaag caagtggaac ttgtacgacc
    40261 tttttaatca attaactaaa acagtaaagg gactgtgaca aaagtcttgt cacagtccct
    40321 tgttacgaat aaatggtgtt ccagaagagg cttcttcgga aataaagcat gaaaaaaatt
    40381 tcatgcagtg aatgcaattt tttgttaaga taaatctcag caaaggataa tggaggaaag
    40441 aacatgattt ttgcttataa caaagcacac gtcggagaca ccttgatggt tattatagcc
    40501 gacgataaag gaacagaaaa cactgttgaa cgcaaatgga atgttgctcg gatcaaagat
    40561 gaacaaggtc aagttgttgc ttggaacttt ttccatatct ccgatcatct aacaatcgaa
    40621 gggaacggcc aagtaagctt aacagatgag gaattagcga aattgaatcg attgatcaaa
    40681 gaagcaggat tcgaagaaca gcttgaagct gatcatgaac cgaagatcgt tgtcgggtac
    40741 gtaaaaacat gcgtcccaca tcctgattct gaccatttat cgattactga gacagaagta
    40801 gacaatggta cagtattgca aatcgtttgc ggtgcaccca atatcgaagc tggacaaaaa
    40861 gttgtcgttg caaaaccagg agctatgatg ccagatggaa tgatgatctg gccaggaact
    40921 ttgcgagggg tagatagcta tggaatgatc tgttccgctc gtgaattgca tttgccaaat
    40981 gctccagcaa aaaaaggaat cttagtattg cctgaggatg ccgtagtcgg agaagcattt
    41041 ccaaaataaa gaggttgtga caagtctttg tcacaacctc ttttttatta attttcacta
    41101 ttttgatttt gttttaatgc cgattgatcg atcgtcaatt caacagtagt tgttttttct
    41161 ttcgagcctt catagtaagt gattttgaca gaatctccca cttttttctt atacaaagca
    41221 gattgaagtt ctacaccaga agaaacttct ttgtcatcga tcttagtaat gacgtcgtat
    41281 tgtttcaatc cagctttttc agccggtgta gctgtttgga catttgtcac gatgacaccg
    41341 ttagttacag atgaaggaat cttcaagatt tgttcttgtt gctgagtaga aacggcagat
    41401 aagtcgacca ttgtgatacc tagagctgga cgtgtcactt ttccgtcttt ttccagttga
    41461 ttgatgatat tcactacatc gttacttgga atagcaaatc ccattccttc tacgcttaca
    41521 ttagaagtag attcagaagt acttgcgatc ttacttgagt tgataccgat aacttgtcct
    41581 tcgatattaa ctaaaggacc accagaattt ccagggttga ttgctgcatc tgtttggatc
    41641 gcattgatat tgactgtttc gttcgattca ttcgtacttg tcacttgtcg gttcaaggaa
    41701 gaaatgattc ctgaagtgac agaatttgca tattcagatc ctagtgggga accaatcgca
    41761 atcgcaggct caccaacctt cagagcactt gagtcaccaa aagaggcaac tgtttcgact
    41821 ttgtctgaat tgatcttcaa caccgctaaa tctgaataag catctgttcc aactaattca
    41881 gcttttacct tcgtaccgtc tttcatcaag acttcaagac cttgctggcc atctactacg
    41941 tgattattcg tgactacata agcagtattc ccgtcttttt tgtagatcac gccgcttcct
    42001 tcactataag cttccaaatt actatcatct gagctgctac tttcttcttg ctgcccgaat
    42061 agtccgccaa aaccgcttga ttggttttga ctttgtaagt tgataacaga aacgacagcg
    42121 tcttgaactt tatctaccgc tttggtgata tcagaatcta cattgacttt gacattttcc
    42181 acaactgttt caccggcaga attctgattg ccgcttgtag cagtagaact attgttgcca
    42241 gtgcccatcg ctgcataaaa aatgccagct gtcagtagtc ctccaacgat ccctccaaca
    42301 agccccagac ctaacttacg ccagaggcta ttatttttac tttttttcat ctttggtgtc
    42361 acattttttc tatccatgtg ttcctcctcc aattacattc atttatctac tgttagtata
    42421 gtcttatttg ttgactttag tctactttga gactgtgaac tttttatgaa aaactttagg
    42481 aaaaatcatt gttttcagat gaaaatattt gattttcttt ttccacagat actgtgtaaa
    42541 actacgcgga taggattgga aaaataaggg ataaaacata tccacagcct gtgtataaag
    42601 tggataaatt cttataaaac gtatgtttgc ctaatgaatg tgatgaaata aggtaaaata
    42661 acactgtttt ctgtggataa acctgtggat gttgtgcata actgttgggg aaatcttttt
    42721 aacaaaaata gccaaaataa acatatagac gaaaaaattg attataataa acaaagtgag
    42781 catgaagacc aaaggagaga aaacatgaat atcaaaatta tctcagtagg aaaattaaaa
    42841 gaaaaatacc tgatccaagg tatcaatgag tatgtcaaac ggctgaatgc ctatgcaaaa
    42901 atcgaattga tcgaagttcc tgacgaaaaa gccccggaga atttaagcga agcgcaaatg
    42961 agacaagtaa aagaaaaaga aggggaacgg atcttagcga aaatcaaaga acaagaatac
    43021 gtctatgcct tagcaattga agggaaaaat cctacaagtg aatcttttgc taaacaaatc
    43081 gaccaattag gtatccaagg aaaaagccac cttgtttttg ttatcggcgg ctctttagga
    43141 ctcagcgaag ccgtcatgaa aagaagcaat gctcagatct cttttggaaa aatgacgtat
    43201 ccacatcaat tgatgcgtct gatccttgtc gagcaaatct accgtgcctt ccggatcaat
    43261 gcgggggctc cgtatcataa ataatgatat ttaagattga atgc
//
LOCUS       DAZBWR010000011        39507 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000011 DAZBWR010000000
VERSION     DAZBWR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.011"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..538)
                     /locus_tag="ACHGT5_000554"
     CDS             complement(<1..538)
                     /locus_tag="ACHGT5_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN7216568.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAE"
     gene            complement(550..1242)
                     /locus_tag="ACHGT5_000555"
     CDS             complement(550..1242)
                     /locus_tag="ACHGT5_000555"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN7216569.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1261..1545)
                     /gene="macP"
                     /locus_tag="ACHGT5_000556"
     CDS             complement(1261..1545)
                     /gene="macP"
                     /locus_tag="ACHGT5_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN7216570.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1558..2121)
                     /locus_tag="ACHGT5_000557"
     CDS             complement(1558..2121)
                     /locus_tag="ACHGT5_000557"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN7216571.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2349..3011
                     /locus_tag="ACHGT5_000558"
     CDS             2349..3011
                     /locus_tag="ACHGT5_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN7216572.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3135..4331
                     /locus_tag="ACHGT5_000559"
     CDS             3135..4331
                     /locus_tag="ACHGT5_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN7216573.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4432..5808)
                     /locus_tag="ACHGT5_000560"
     CDS             complement(4432..5808)
                     /locus_tag="ACHGT5_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN7216574.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5845..6711)
                     /locus_tag="ACHGT5_000561"
     CDS             complement(5845..6711)
                     /locus_tag="ACHGT5_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN7216575.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6800..7825)
                     /locus_tag="ACHGT5_000562"
     CDS             complement(6800..7825)
                     /locus_tag="ACHGT5_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN7216576.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7843..8517)
                     /locus_tag="ACHGT5_000563"
     CDS             complement(7843..8517)
                     /locus_tag="ACHGT5_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7216577.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8701..9594)
                     /locus_tag="ACHGT5_000564"
     CDS             complement(8701..9594)
                     /locus_tag="ACHGT5_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN7216578.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9668..11365)
                     /locus_tag="ACHGT5_000565"
     CDS             complement(9668..11365)
                     /locus_tag="ACHGT5_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN7216579.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11358..12671)
                     /locus_tag="ACHGT5_000566"
     CDS             complement(11358..12671)
                     /locus_tag="ACHGT5_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN7216580.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12671..16612)
                     /locus_tag="ACHGT5_000567"
     CDS             complement(12671..16612)
                     /locus_tag="ACHGT5_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN7216581.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16721..17179)
                     /locus_tag="ACHGT5_000568"
     CDS             complement(16721..17179)
                     /locus_tag="ACHGT5_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN7216582.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17477..17995)
                     /locus_tag="ACHGT5_000569"
     CDS             complement(17477..17995)
                     /locus_tag="ACHGT5_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN7216583.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17997..18614)
                     /locus_tag="ACHGT5_000570"
     CDS             complement(17997..18614)
                     /locus_tag="ACHGT5_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN7216584.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18611..19465)
                     /locus_tag="ACHGT5_000571"
     CDS             complement(18611..19465)
                     /locus_tag="ACHGT5_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7216585.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19462..20373)
                     /locus_tag="ACHGT5_000572"
     CDS             complement(19462..20373)
                     /locus_tag="ACHGT5_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7216586.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20414..21748)
                     /locus_tag="ACHGT5_000573"
     CDS             complement(20414..21748)
                     /locus_tag="ACHGT5_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7216587.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21956..22786
                     /locus_tag="ACHGT5_000574"
     CDS             21956..22786
                     /locus_tag="ACHGT5_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN7216588.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23030..23279)
                     /locus_tag="ACHGT5_000575"
                     /pseudo
     CDS             complement(23030..23279)
                     /locus_tag="ACHGT5_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23470..23664)
                     /locus_tag="ACHGT5_000576"
     CDS             complement(23470..23664)
                     /locus_tag="ACHGT5_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216589.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24169..24354)
                     /locus_tag="ACHGT5_000577"
     CDS             complement(24169..24354)
                     /locus_tag="ACHGT5_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216590.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25204..25545)
                     /locus_tag="ACHGT5_000578"
     CDS             complement(25204..25545)
                     /locus_tag="ACHGT5_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN7216591.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25545..25775)
                     /locus_tag="ACHGT5_000579"
     CDS             complement(25545..25775)
                     /locus_tag="ACHGT5_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN7216592.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26062..26400
                     /locus_tag="ACHGT5_000580"
     CDS             26062..26400
                     /locus_tag="ACHGT5_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216593.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26605..27167)
                     /locus_tag="ACHGT5_000581"
                     /pseudo
     CDS             complement(26605..27167)
                     /locus_tag="ACHGT5_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27899..28480)
                     /locus_tag="ACHGT5_000582"
     CDS             complement(27899..28480)
                     /locus_tag="ACHGT5_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7216594.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28663..29289)
                     /locus_tag="ACHGT5_000583"
     CDS             complement(28663..29289)
                     /locus_tag="ACHGT5_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN7216595.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29311..30642)
                     /locus_tag="ACHGT5_000584"
     CDS             complement(29311..30642)
                     /locus_tag="ACHGT5_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN7216596.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30797..31198)
                     /locus_tag="ACHGT5_000585"
     CDS             complement(30797..31198)
                     /locus_tag="ACHGT5_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN7216597.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31284..31490)
                     /locus_tag="ACHGT5_000586"
     CDS             complement(31284..31490)
                     /locus_tag="ACHGT5_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216598.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31585..33087)
                     /locus_tag="ACHGT5_000587"
     CDS             complement(31585..33087)
                     /locus_tag="ACHGT5_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN7216599.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33105..33560)
                     /gene="argR"
                     /locus_tag="ACHGT5_000588"
     CDS             complement(33105..33560)
                     /gene="argR"
                     /locus_tag="ACHGT5_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN7216600.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33658..34275)
                     /locus_tag="ACHGT5_000589"
     CDS             complement(33658..34275)
                     /locus_tag="ACHGT5_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN7216601.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34496..35869
                     /locus_tag="ACHGT5_000590"
     CDS             34496..35869
                     /locus_tag="ACHGT5_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7216602.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35944..37056
                     /locus_tag="ACHGT5_000591"
     CDS             35944..37056
                     /locus_tag="ACHGT5_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN7216603.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37128..38765)
                     /locus_tag="ACHGT5_000592"
     CDS             complement(37128..38765)
                     /locus_tag="ACHGT5_000592"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN7216604.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38933..>39507)
                     /locus_tag="ACHGT5_000593"
     CDS             complement(38933..>39507)
                     /locus_tag="ACHGT5_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN7216605.1"
                     /translation="FAAGATAMFVGTTNAHADEVYTVKSGDSLSKISQKFAGDNSMID
                     AIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTETATQEHTYVAPVE
                     TVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQLDASYLNGDYSAA
                     NQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11339 a         8417 c         6879 g        12872 t
ORIGIN      
        1 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
       61 aacttgccac ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
      121 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
      181 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
      241 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
      301 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
      361 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
      421 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
      481 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
      541 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
      601 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
      661 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
      721 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
      781 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
      841 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
      901 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
      961 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
     1021 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
     1081 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
     1141 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
     1201 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
     1261 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
     1321 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
     1381 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
     1441 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
     1501 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
     1561 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
     1621 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
     1681 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
     1741 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
     1801 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
     1861 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
     1921 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
     1981 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
     2041 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
     2101 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
     2161 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
     2221 ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta
     2281 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
     2341 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
     2401 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
     2461 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
     2521 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
     2581 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
     2641 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
     2701 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
     2761 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
     2821 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
     2881 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
     2941 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
     3001 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
     3061 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
     3121 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
     3181 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
     3241 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
     3301 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
     3361 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
     3421 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
     3481 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
     3541 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
     3601 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
     3661 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
     3721 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
     3781 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
     3841 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
     3901 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
     3961 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
     4021 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
     4081 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
     4141 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
     4201 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
     4261 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
     4321 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
     4381 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
     4441 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
     4501 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
     4561 aatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
     4621 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
     4681 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
     4741 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
     4801 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
     4861 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
     4921 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
     4981 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
     5041 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
     5101 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
     5161 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
     5221 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
     5281 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
     5341 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
     5401 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
     5461 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
     5521 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
     5581 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
     5641 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
     5701 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
     5761 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
     5821 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
     5881 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
     5941 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
     6001 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
     6061 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
     6121 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
     6181 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
     6241 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
     6301 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
     6361 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
     6421 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
     6481 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
     6541 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
     6601 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
     6661 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
     6721 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
     6781 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
     6841 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
     6901 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
     6961 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
     7021 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
     7081 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
     7141 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
     7201 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
     7261 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
     7321 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
     7381 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
     7441 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
     7501 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
     7561 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
     7621 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
     7681 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
     7741 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
     7801 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
     7861 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
     7921 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
     7981 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
     8041 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
     8101 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
     8161 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
     8221 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
     8281 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
     8341 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
     8401 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
     8461 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
     8521 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
     8581 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
     8641 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
     8701 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
     8761 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
     8821 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
     8881 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
     8941 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
     9001 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
     9061 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
     9121 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
     9181 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
     9241 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
     9301 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
     9361 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
     9421 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
     9481 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
     9541 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
     9601 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
     9661 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
     9721 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
     9781 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
     9841 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
     9901 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
     9961 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    10021 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    10081 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    10141 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    10201 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    10261 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    10321 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    10381 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    10441 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    10501 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    10561 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    10621 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    10681 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    10741 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    10801 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    10861 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    10921 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    10981 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    11041 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    11101 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    11161 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    11221 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    11281 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    11341 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    11401 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    11461 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    11521 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    11581 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    11641 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    11701 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    11761 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    11821 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    11881 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    11941 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    12001 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    12061 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    12121 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    12181 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    12241 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    12301 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    12361 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    12421 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    12481 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    12541 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    12601 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    12661 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    12721 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    12781 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    12841 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    12901 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    12961 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    13021 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    13081 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    13141 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    13201 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    13261 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    13321 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    13381 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    13441 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    13501 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    13561 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    13621 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    13681 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    13741 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    13801 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    13861 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    13921 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    13981 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    14041 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    14101 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    14161 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    14221 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    14281 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    14341 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    14401 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    14461 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    14521 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    14581 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    14641 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    14701 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    14761 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    14821 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    14881 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    14941 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    15001 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    15061 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    15121 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    15181 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    15241 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    15301 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    15361 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    15421 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    15481 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    15541 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    15601 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    15661 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    15721 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    15781 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    15841 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    15901 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    15961 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    16021 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    16081 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    16141 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    16201 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    16261 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    16321 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    16381 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    16441 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    16501 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    16561 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    16621 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    16681 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    16741 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    16801 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    16861 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    16921 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    16981 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    17041 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    17101 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    17161 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    17221 caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa
    17281 tgatatcctt tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta
    17341 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
    17401 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
    17461 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
    17521 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
    17581 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
    17641 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
    17701 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
    17761 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
    17821 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
    17881 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
    17941 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
    18001 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
    18061 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
    18121 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
    18181 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
    18241 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
    18301 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
    18361 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
    18421 gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga
    18481 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
    18541 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
    18601 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
    18661 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
    18721 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
    18781 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
    18841 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
    18901 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
    18961 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
    19021 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
    19081 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
    19141 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
    19201 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
    19261 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
    19321 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
    19381 tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac
    19441 tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg
    19501 tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat
    19561 agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat gcagattata
    19621 aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg
    19681 aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat
    19741 taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga
    19801 ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc
    19861 agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca
    19921 agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga
    19981 aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa
    20041 agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac
    20101 actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga
    20161 tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt
    20221 catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc
    20281 tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc
    20341 ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac
    20401 agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg
    20461 ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta
    20521 atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt
    20581 cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat
    20641 tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta
    20701 cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt
    20761 atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt
    20821 ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt
    20881 aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac
    20941 cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt
    21001 cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt
    21061 tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc
    21121 ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg
    21181 ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt
    21241 taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag
    21301 gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt
    21361 tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat
    21421 ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag
    21481 tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat
    21541 tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc
    21601 agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac
    21661 tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca
    21721 gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc
    21781 cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct
    21841 aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt
    21901 ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt
    21961 tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc
    22021 gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta
    22081 cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat
    22141 tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga
    22201 gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat
    22261 tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga
    22321 cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga
    22381 ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga
    22441 aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta
    22501 tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt
    22561 agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa
    22621 cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg
    22681 cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga
    22741 accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag
    22801 aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat
    22861 aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc
    22921 ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc
    22981 acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta
    23041 cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg
    23101 tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc
    23161 caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat
    23221 tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc
    23281 tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg
    23341 tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg
    23401 cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg
    23461 caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa
    23521 taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat
    23581 cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata
    23641 gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa
    23701 attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt
    23761 tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa
    23821 tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta
    23881 tacataacct tgatacttcg accacttata tatcgaacac acaaatacct atccattgca
    23941 ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt
    24001 actcttagta aagtatctga actattaatt tttaaactat catttttatc ctcaactaca
    24061 tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga
    24121 attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat
    24181 tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt
    24241 aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat
    24301 ataccctata aaagctaata agatggttag gaatccttta gaatttttac tcattcatag
    24361 acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag
    24421 tgactcaaaa taaaaaaaac cttacattat actggaatga actgtattga tagtgtacct
    24481 aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga
    24541 tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct
    24601 ttgagattct actttggttc actatctgac ccaattgcac attttcgatc attacaagta
    24661 ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa
    24721 tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat
    24781 gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga
    24841 tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa
    24901 gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt
    24961 attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca
    25021 aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt
    25081 tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact
    25141 tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa
    25201 tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt
    25261 ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac
    25321 ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt
    25381 ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac
    25441 gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca
    25501 tacaatatca ccttttttag gaatataaat tctttcttct gacactataa aatttctctc
    25561 ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca
    25621 ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg
    25681 atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca
    25741 gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta
    25801 tactatataa cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt
    25861 gtttaaagta tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct
    25921 ttaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac
    25981 tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca
    26041 aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc
    26101 tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt
    26161 cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa
    26221 gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat
    26281 gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat
    26341 acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa
    26401 tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga
    26461 agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc
    26521 atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc
    26581 tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg
    26641 agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt
    26701 caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat
    26761 tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac
    26821 tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa
    26881 acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca
    26941 cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc
    27001 ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact
    27061 tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa
    27121 ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc
    27181 aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt
    27241 tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg
    27301 ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt
    27361 gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa
    27421 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa
    27481 atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
    27541 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta
    27601 tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc actaaacggt
    27661 gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    27721 gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt
    27781 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat
    27841 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt
    27901 aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta
    27961 gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg
    28021 ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat
    28081 tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt
    28141 catccactgt actcaactca ataatcaaat tacctacagg acagccatga taagtgatct
    28201 gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt
    28261 ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga
    28321 tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg
    28381 tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa
    28441 gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt
    28501 tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa
    28561 gactgtgcca aattatctga cacagccctc actataaaac tgttctcata tagctagatg
    28621 ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa
    28681 tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc
    28741 cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact
    28801 ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat
    28861 tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa
    28921 gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat
    28981 aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg
    29041 ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta
    29101 aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc
    29161 aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc
    29221 gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt
    29281 attgaacatg gtggattctc cattcgttat ttattttaat acatcattta gagcttcact
    29341 cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat
    29401 agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag
    29461 aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac
    29521 ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc
    29581 tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta
    29641 aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat
    29701 gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat
    29761 cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc
    29821 tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt
    29881 tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac
    29941 ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat
    30001 tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag
    30061 aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc
    30121 aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg
    30181 atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa
    30241 aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc
    30301 accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac
    30361 tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa
    30421 tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa
    30481 tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt
    30541 ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc
    30601 ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt
    30661 ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc caaatcatct
    30721 gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc
    30781 gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt
    30841 tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga
    30901 tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca tctaaattat
    30961 aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat
    31021 gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg
    31081 ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct
    31141 cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg
    31201 tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa
    31261 ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt
    31321 ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata
    31381 gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag
    31441 ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc
    31501 ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc
    31561 aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg
    31621 tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc
    31681 tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc
    31741 gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc
    31801 tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg
    31861 ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt
    31921 gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt
    31981 tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct
    32041 tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat
    32101 tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct
    32161 ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa
    32221 tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact
    32281 gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc
    32341 taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt
    32401 aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt
    32461 caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca
    32521 accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc
    32581 ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat
    32641 tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt
    32701 aaagaatttt attccattat aataataggg aagatggcta gcagtcagca taaccgcagc
    32761 atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc
    32821 gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc
    32881 tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt
    32941 tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc
    33001 tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc
    33061 attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt
    33121 tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta
    33181 tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt
    33241 accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata
    33301 cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc
    33361 tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt
    33421 tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc
    33481 gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct
    33541 tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt
    33601 aatatttctt catattttgg tattttttac ttttattttt tgttttcatt caggttgcta
    33661 acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg
    33721 atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat
    33781 gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat
    33841 ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg
    33901 aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg
    33961 tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt
    34021 ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat
    34081 cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac
    34141 tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt
    34201 tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca
    34261 ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc
    34321 tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag
    34381 tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta
    34441 tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac
    34501 aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc
    34561 gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga
    34621 tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat
    34681 tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc
    34741 aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct
    34801 attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca
    34861 tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt
    34921 gacaacggcc attgctccag caattggccc cacttatgga ggcattatga cttcacattt
    34981 tacatggaat catatcttcc tgtttcttgt gcccgttttg tttctttctt tgattatcgg
    35041 ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt
    35101 tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt
    35161 ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag
    35221 taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt
    35281 gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc
    35341 gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc
    35401 cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg
    35461 tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt
    35521 cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat
    35581 cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga
    35641 actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc
    35701 tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga
    35761 agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt
    35821 aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac
    35881 ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt
    35941 tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac
    36001 ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa
    36061 gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt
    36121 ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa
    36181 acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg
    36241 gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt
    36301 tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc
    36361 agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca
    36421 caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg
    36481 ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg
    36541 gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg
    36601 cagctcttag catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt
    36661 gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat
    36721 gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg attgactaag
    36781 agtacagaac agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt
    36841 gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat
    36901 ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc
    36961 ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag
    37021 gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga
    37081 tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc
    37141 tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga
    37201 gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta
    37261 tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca
    37321 aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca
    37381 ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt
    37441 ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga
    37501 ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg
    37561 tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc
    37621 ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg
    37681 ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg
    37741 tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca
    37801 ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta
    37861 tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca
    37921 tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat
    37981 tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat
    38041 tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc
    38101 gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta
    38161 tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca
    38221 caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc
    38281 ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct
    38341 ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta
    38401 tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg
    38461 ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg
    38521 cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt
    38581 gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc
    38641 ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga
    38701 atactggata acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta
    38761 ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga
    38821 gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac
    38881 cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc
    38941 agccgtttgc taaccagaat gttttagcag catcccatga accataacga gatgttacat
    39001 attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat
    39061 ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt
    39121 tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag
    39181 caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt
    39241 cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg
    39301 aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt
    39361 tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata
    39421 atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa
    39481 acattgcagt agcaccagca gcaaaag
//
LOCUS       DAZBWR010000012        38652 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000012 DAZBWR010000000
VERSION     DAZBWR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.012"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1274
                     /gene="pdhA"
                     /locus_tag="ACHGT5_000594"
     CDS             165..1274
                     /gene="pdhA"
                     /locus_tag="ACHGT5_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN7216606.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1277..2254
                     /locus_tag="ACHGT5_000595"
     CDS             1277..2254
                     /locus_tag="ACHGT5_000595"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN7216607.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2279..3922
                     /locus_tag="ACHGT5_000596"
     CDS             2279..3922
                     /locus_tag="ACHGT5_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN7216608.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            3930..5336
                     /gene="lpdA"
                     /locus_tag="ACHGT5_000597"
     CDS             3930..5336
                     /gene="lpdA"
                     /locus_tag="ACHGT5_000597"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN7216609.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5498..5773
                     /locus_tag="ACHGT5_000598"
     CDS             5498..5773
                     /locus_tag="ACHGT5_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN7216610.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5779..6546
                     /locus_tag="ACHGT5_000599"
     CDS             5779..6546
                     /locus_tag="ACHGT5_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN7216611.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6714..8543
                     /gene="typA"
                     /locus_tag="ACHGT5_000600"
     CDS             6714..8543
                     /gene="typA"
                     /locus_tag="ACHGT5_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN7216612.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            8804..10576
                     /gene="recQ"
                     /locus_tag="ACHGT5_000601"
     CDS             8804..10576
                     /gene="recQ"
                     /locus_tag="ACHGT5_000601"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN7216613.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10690..10992
                     /locus_tag="ACHGT5_000602"
     CDS             10690..10992
                     /locus_tag="ACHGT5_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN7216614.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            10995..12158
                     /locus_tag="ACHGT5_000603"
     CDS             10995..12158
                     /locus_tag="ACHGT5_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN7216615.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12184..15612
                     /locus_tag="ACHGT5_000604"
     CDS             12184..15612
                     /locus_tag="ACHGT5_000604"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN7216616.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15633..16757
                     /locus_tag="ACHGT5_000605"
     CDS             15633..16757
                     /locus_tag="ACHGT5_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN7216617.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16738..17184
                     /locus_tag="ACHGT5_000606"
     CDS             16738..17184
                     /locus_tag="ACHGT5_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN7216618.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17168..17506
                     /locus_tag="ACHGT5_000607"
     CDS             17168..17506
                     /locus_tag="ACHGT5_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN7216619.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17625..18185
                     /gene="rsmD"
                     /locus_tag="ACHGT5_000608"
     CDS             17625..18185
                     /gene="rsmD"
                     /locus_tag="ACHGT5_000608"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN7216620.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18182..18673
                     /gene="coaD"
                     /locus_tag="ACHGT5_000609"
     CDS             18182..18673
                     /gene="coaD"
                     /locus_tag="ACHGT5_000609"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN7216621.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18663..19706
                     /locus_tag="ACHGT5_000610"
     CDS             18663..19706
                     /locus_tag="ACHGT5_000610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN7216622.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            19865..20593
                     /locus_tag="ACHGT5_000611"
     CDS             19865..20593
                     /locus_tag="ACHGT5_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN7216623.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20709..20954
                     /locus_tag="ACHGT5_000612"
     CDS             20709..20954
                     /locus_tag="ACHGT5_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN7216624.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            20951..21169
                     /locus_tag="ACHGT5_000613"
     CDS             20951..21169
                     /locus_tag="ACHGT5_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN7216625.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21305..21844
                     /locus_tag="ACHGT5_000614"
     CDS             21305..21844
                     /locus_tag="ACHGT5_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN7216626.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            21837..24110
                     /locus_tag="ACHGT5_000615"
     CDS             21837..24110
                     /locus_tag="ACHGT5_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN7216627.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24148..25194
                     /gene="holA"
                     /locus_tag="ACHGT5_000616"
     CDS             24148..25194
                     /gene="holA"
                     /locus_tag="ACHGT5_000616"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN7216628.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25283..25537)
                     /gene="rpsT"
                     /locus_tag="ACHGT5_000617"
     CDS             complement(25283..25537)
                     /gene="rpsT"
                     /locus_tag="ACHGT5_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN7216629.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(25937..26206)
                     /locus_tag="ACHGT5_000618"
     CDS             complement(25937..26206)
                     /locus_tag="ACHGT5_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216630.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26468..26596)
                     /locus_tag="ACHGT5_000619"
     CDS             complement(26468..26596)
                     /locus_tag="ACHGT5_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216631.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(26676..27131)
                     /locus_tag="ACHGT5_000620"
     CDS             complement(26676..27131)
                     /locus_tag="ACHGT5_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN7216632.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27269..29197)
                     /locus_tag="ACHGT5_000621"
     CDS             complement(27269..29197)
                     /locus_tag="ACHGT5_000621"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN7216633.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(29394..30227)
                     /locus_tag="ACHGT5_000622"
     CDS             complement(29394..30227)
                     /locus_tag="ACHGT5_000622"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN7216634.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(30419..31123)
                     /locus_tag="ACHGT5_000623"
     CDS             complement(30419..31123)
                     /locus_tag="ACHGT5_000623"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN7216635.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(31146..31772)
                     /locus_tag="ACHGT5_000624"
     CDS             complement(31146..31772)
                     /locus_tag="ACHGT5_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN7216636.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            32098..34473
                     /locus_tag="ACHGT5_000625"
     CDS             32098..34473
                     /locus_tag="ACHGT5_000625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN7216637.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(34497..34988)
                     /locus_tag="ACHGT5_000626"
     CDS             complement(34497..34988)
                     /locus_tag="ACHGT5_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN7216638.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            35175..36095
                     /locus_tag="ACHGT5_000627"
     CDS             35175..36095
                     /locus_tag="ACHGT5_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN7216639.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            36249..36593
                     /locus_tag="ACHGT5_000628"
     CDS             36249..36593
                     /locus_tag="ACHGT5_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN7216640.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(36783..37283)
                     /locus_tag="ACHGT5_000629"
     CDS             complement(36783..37283)
                     /locus_tag="ACHGT5_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216641.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            37811..37894
                     /locus_tag="ACHGT5_000630"
     tRNA            37811..37894
                     /locus_tag="ACHGT5_000630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37845..37847,aa:Leu,seq:cag)
     gene            complement(38218..>38652)
                     /locus_tag="ACHGT5_000631"
     CDS             complement(38218..>38652)
                     /locus_tag="ACHGT5_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN7216642.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        12790 a         6789 c         8015 g        11058 t
ORIGIN      
        1 cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg
       61 ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca
      121 gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag
      181 caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag
      241 cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg
      301 aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag
      361 cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc
      421 agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg
      481 tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac
      541 atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg
      601 gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa
      661 atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa
      721 tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg
      781 cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg
      841 cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta tatactgtat
      901 ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga
      961 cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag
     1021 acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg
     1081 aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa
     1141 tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga
     1201 aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga
     1261 aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc
     1321 acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg
     1381 aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga
     1441 tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt
     1501 tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt
     1561 agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac ccatcacaat
     1621 tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg
     1681 tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa
     1741 aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa
     1801 actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa
     1861 agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg
     1921 tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga
     1981 tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg
     2041 tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc
     2101 tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc
     2161 tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat
     2221 cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat
     2281 ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa
     2341 atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa
     2401 tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa atgtgattgt
     2461 tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg caccaggaca
     2521 cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag ctaaaccggc
     2581 tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag tcttccaatt
     2641 taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa
     2701 accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg acaaatcagt
     2761 agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac
     2821 tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc
     2881 gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc
     2941 aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca
     3001 atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag
     3061 agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa
     3121 atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc
     3181 agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat
     3241 gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca
     3301 cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa
     3361 agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt
     3421 aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga
     3481 aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc acggactata
     3541 tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa
     3601 tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac
     3661 aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta
     3721 tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga
     3781 aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt
     3841 agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga
     3901 gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag
     3961 atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa
     4021 tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg
     4081 gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt
     4141 ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat
     4201 ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac
     4261 ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca
     4321 ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc
     4381 gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat
     4441 tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg
     4501 aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac
     4561 aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa
     4621 aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg
     4681 tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg
     4741 tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag
     4801 taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct
     4861 atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa
     4921 aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg
     4981 cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag
     5041 atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat
     5101 cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta
     5161 ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa
     5221 tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg
     5281 gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat atttgatttg
     5341 aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa
     5401 taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg
     5461 ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact
     5521 tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga
     5581 acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa
     5641 aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc
     5701 cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag
     5761 aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa
     5821 gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga
     5881 acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat
     5941 tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat
     6001 ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa
     6061 ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc
     6121 atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa
     6181 aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg
     6241 aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg
     6301 gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac
     6361 attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa
     6421 gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa
     6481 tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa
     6541 ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt
     6601 tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag
     6661 tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt
     6721 atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat
     6781 tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac
     6841 gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca
     6901 cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact
     6961 tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg
     7021 cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata
     7081 tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg
     7141 tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc
     7201 ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc
     7261 aagaaccaac catggcacct atttttgata caattattga aaaaatccct gctcctgtcg
     7321 ataacagcga cgaaccatta caattccaag tttcattact tgactacaac gattatgtag
     7381 gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat
     7441 taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg
     7501 gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta
     7561 tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta
     7621 tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg
     7681 ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac
     7741 ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt
     7801 ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg
     7861 aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac
     7921 cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct
     7981 tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt
     8041 tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga
     8101 cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc
     8161 aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt
     8221 attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat
     8281 atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga
     8341 aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa
     8401 aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg
     8461 aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa
     8521 aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata
     8581 aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa
     8641 tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt
     8701 ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt
     8761 cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa
     8821 acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat
     8881 tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca
     8941 gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat
     9001 gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc
     9061 gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt
     9121 actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg
     9181 gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt
     9241 ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat
     9301 tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa
     9361 aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt
     9421 aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca
     9481 atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa
     9541 aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga
     9601 aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg
     9661 tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct
     9721 agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat
     9781 cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag
     9841 agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta
     9901 tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg
     9961 caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca
    10021 gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa
    10081 agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac
    10141 ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat
    10201 atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag
    10261 agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca
    10321 gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga
    10381 tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga
    10441 aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca
    10501 aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa
    10561 cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc
    10621 tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg
    10681 taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca
    10741 gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa
    10801 gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct
    10861 gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga
    10921 gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc
    10981 aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct
    11041 tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt
    11101 atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt
    11161 gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa
    11221 accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt
    11281 tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc
    11341 aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa
    11401 gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta
    11461 tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt
    11521 tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag
    11581 gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa
    11641 agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt
    11701 tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg
    11761 gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag
    11821 cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt
    11881 tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa
    11941 aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat
    12001 ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt
    12061 taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca
    12121 tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt
    12181 tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct
    12241 tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga atattccgta
    12301 caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg
    12361 tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat
    12421 ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc
    12481 atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca
    12541 aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct
    12601 tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca
    12661 gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag
    12721 ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt
    12781 gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga
    12841 aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta
    12901 gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct
    12961 gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa
    13021 ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca
    13081 gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat
    13141 ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt
    13201 caatgccgaa tcactacaga agacccgttg aataactttt taccagacac agggaaaatc
    13261 gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg
    13321 ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct
    13381 acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga
    13441 gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga
    13501 aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga
    13561 gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt
    13621 atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca
    13681 agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc
    13741 gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat
    13801 caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact
    13861 ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg
    13921 gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg
    13981 cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat
    14041 cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt
    14101 cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg
    14161 gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt
    14221 cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt
    14281 gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt
    14341 ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc
    14401 agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac
    14461 gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat
    14521 gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac
    14581 cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct
    14641 gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag
    14701 gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc
    14761 aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg
    14821 gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca
    14881 ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc
    14941 ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt
    15001 ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa
    15061 attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt
    15121 ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca
    15181 tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg
    15241 cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc
    15301 ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg
    15361 gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct
    15421 ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg
    15481 atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc
    15541 gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg
    15601 actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga
    15661 ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg
    15721 aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg
    15781 agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg
    15841 attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg
    15901 aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg
    15961 ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc
    16021 aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag
    16081 aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata
    16141 acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt
    16201 atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc
    16261 cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa
    16321 aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa
    16381 cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg
    16441 aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga
    16501 cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag
    16561 aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt
    16621 ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca
    16681 ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg
    16741 atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct
    16801 gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa
    16861 gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa
    16921 aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga
    16981 aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca
    17041 cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa
    17101 atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt
    17161 aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt
    17221 atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt
    17281 ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga
    17341 agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat
    17401 cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga
    17461 aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag
    17521 cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt
    17581 tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag
    17641 gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag
    17701 ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat
    17761 tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc
    17821 atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa
    17881 ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac
    17941 ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac
    18001 aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag
    18061 ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc
    18121 aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc
    18181 aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac
    18241 ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc
    18301 aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca
    18361 tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga
    18421 gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg
    18481 agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc
    18541 aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg
    18601 agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc
    18661 aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca
    18721 ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc
    18781 tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta
    18841 cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt
    18901 ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc
    18961 aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac
    19021 ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa
    19081 catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa
    19141 gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa
    19201 tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt
    19261 taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat
    19321 cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt
    19381 tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg
    19441 caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa
    19501 aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt
    19561 cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg
    19621 ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc
    19681 ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt
    19741 tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa
    19801 aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt
    19861 ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat
    19921 agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat
    19981 agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga
    20041 aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc
    20101 atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat
    20161 caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga
    20221 tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc
    20281 tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga
    20341 acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc
    20401 caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc
    20461 agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga
    20521 agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt
    20581 tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga
    20641 tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag
    20701 gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc
    20761 aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag
    20821 ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc
    20881 tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa
    20941 tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat
    21001 cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga
    21061 atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat
    21121 acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga
    21181 cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa
    21241 agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata
    21301 aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa
    21361 gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc
    21421 tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat
    21481 cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg
    21541 ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca
    21601 gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg
    21661 tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga
    21721 ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca
    21781 agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt
    21841 ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga
    21901 ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt
    21961 ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca
    22021 taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat
    22081 ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg
    22141 aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag
    22201 cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg
    22261 agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca
    22321 ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa
    22381 atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg
    22441 aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag
    22501 gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat
    22561 cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat
    22621 ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg
    22681 caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca
    22741 aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt
    22801 tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt
    22861 ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc
    22921 tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag
    22981 gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt
    23041 tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg
    23101 gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac
    23161 gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt
    23221 tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga
    23281 ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca
    23341 gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt
    23401 tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata
    23461 tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg
    23521 acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa
    23581 tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc
    23641 aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg
    23701 atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc
    23761 tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg
    23821 aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc
    23881 acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga
    23941 ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac
    24001 tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct
    24061 ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag taattctcat
    24121 ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta
    24181 caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt
    24241 ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac
    24301 ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca
    24361 gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta
    24421 acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg
    24481 aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa
    24541 cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg
    24601 aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg
    24661 gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg
    24721 agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa
    24781 gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt
    24841 ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa
    24901 gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa
    24961 tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca
    25021 tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag
    25081 ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa
    25141 ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa
    25201 agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa
    25261 aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc
    25321 ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag
    25381 cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt
    25441 tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg
    25501 tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa
    25561 gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct
    25621 ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca
    25681 gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag
    25741 cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg
    25801 gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac
    25861 gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc
    25921 ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt
    25981 ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca
    26041 gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca
    26101 ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt
    26161 cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt
    26221 cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg
    26281 atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc
    26341 caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt
    26401 acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat
    26461 aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag
    26521 ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact
    26581 ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg
    26641 gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt
    26701 aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag
    26761 acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc
    26821 aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag
    26881 ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata
    26941 gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc
    27001 caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct
    27061 atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc
    27121 caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa
    27181 agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa
    27241 taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata
    27301 gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac
    27361 gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta
    27421 gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa
    27481 taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt
    27541 tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt
    27601 gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac
    27661 caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt
    27721 ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg
    27781 tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa
    27841 ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct
    27901 gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg
    27961 caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt
    28021 gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt
    28081 ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg
    28141 tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa
    28201 agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg
    28261 cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg
    28321 cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag
    28381 aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc caacctagga
    28441 aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat
    28501 tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta
    28561 attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg
    28621 gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca
    28681 cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat
    28741 ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa
    28801 catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat
    28861 tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc
    28921 ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac
    28981 caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt
    29041 tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg
    29101 gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc
    29161 cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa
    29221 acaaggatag cataataaaa atgtaattga taataataac agctaaatga gaacgataat
    29281 ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa
    29341 agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt
    29401 gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg
    29461 gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca
    29521 aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct
    29581 tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag
    29641 aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc
    29701 gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga
    29761 agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg
    29821 cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag
    29881 aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt
    29941 atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat
    30001 ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc
    30061 cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg
    30121 aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct
    30181 tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt
    30241 cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt
    30301 aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca
    30361 aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt
    30421 atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc
    30481 cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc
    30541 cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat
    30601 gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa
    30661 atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta
    30721 agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat
    30781 atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca tatccacctt
    30841 caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc
    30901 ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag
    30961 aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt
    31021 gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca
    31081 tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag
    31141 tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg
    31201 ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta
    31261 tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac
    31321 agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc
    31381 cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat
    31441 tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa
    31501 agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt
    31561 tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc
    31621 aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct
    31681 tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca
    31741 atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt
    31801 ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc
    31861 tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa
    31921 agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa
    31981 attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag
    32041 ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg
    32101 atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt
    32161 atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag
    32221 ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa
    32281 ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt
    32341 atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa
    32401 caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat
    32461 gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg
    32521 agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa
    32581 gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg
    32641 atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct
    32701 gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga
    32761 aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag
    32821 atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca
    32881 ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc
    32941 tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat
    33001 cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc
    33061 atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt
    33121 atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta
    33181 agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat
    33241 aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa
    33301 cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta
    33361 ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat
    33421 caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa
    33481 gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt
    33541 gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc
    33601 gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt
    33661 gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc
    33721 tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct
    33781 ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa
    33841 agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc
    33901 gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc
    33961 agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa
    34021 cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc
    34081 gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt
    34141 atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat
    34201 tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa
    34261 gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg
    34321 gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca
    34381 gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca
    34441 aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa
    34501 cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca
    34561 gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct
    34621 tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg
    34681 attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg
    34741 gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct
    34801 ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat
    34861 ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc
    34921 tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct
    34981 tttgccatag gaaccctcct gatatacata taaatattta ccatacatct atcttatcat
    35041 aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt
    35101 ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc
    35161 ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta
    35221 ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg
    35281 tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt
    35341 ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat
    35401 tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg
    35461 tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg
    35521 ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta
    35581 caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg
    35641 gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact
    35701 gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca
    35761 tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa
    35821 cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct
    35881 attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc
    35941 ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt
    36001 cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat
    36061 tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa
    36121 tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta
    36181 aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt
    36241 gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag
    36301 aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca
    36361 atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga
    36421 tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg
    36481 cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat
    36541 ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt
    36601 ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa
    36661 gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt
    36721 aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt
    36781 ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc
    36841 ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc
    36901 tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat
    36961 ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat
    37021 gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct
    37081 aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt
    37141 tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata
    37201 aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct
    37261 tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt
    37321 tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg
    37381 tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg
    37441 ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa
    37501 ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct
    37561 gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag
    37621 agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct
    37681 caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta
    37741 cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa
    37801 aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg
    37861 tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg
    37921 tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt
    37981 taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt
    38041 aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc
    38101 aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat
    38161 aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta
    38221 gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt
    38281 aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga
    38341 agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga
    38401 ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc
    38461 tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac
    38521 cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg
    38581 aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg
    38641 ttgtcctgag ta
//
LOCUS       DAZBWR010000013        34952 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000013 DAZBWR010000000
VERSION     DAZBWR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.013"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACHGT5_000632"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACHGT5_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN7216643.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACHGT5_000633"
     CDS             complement(2017..2919)
                     /locus_tag="ACHGT5_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN7216644.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACHGT5_000634"
     CDS             complement(2916..3608)
                     /locus_tag="ACHGT5_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN7216645.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACHGT5_000635"
     CDS             complement(3651..4292)
                     /locus_tag="ACHGT5_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN7216646.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="ACHGT5_000636"
     CDS             complement(4330..4644)
                     /locus_tag="ACHGT5_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216647.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACHGT5_000637"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACHGT5_000637"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFN7216648.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACHGT5_000638"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACHGT5_000638"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN7216649.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="ACHGT5_000639"
     CDS             complement(5981..6913)
                     /locus_tag="ACHGT5_000639"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN7216650.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="ACHGT5_000640"
     CDS             complement(6910..7686)
                     /locus_tag="ACHGT5_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN7216651.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACHGT5_000641"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACHGT5_000641"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN7216652.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="ACHGT5_000642"
     CDS             complement(10930..12006)
                     /locus_tag="ACHGT5_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN7216653.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="ACHGT5_000643"
     CDS             complement(12011..13291)
                     /locus_tag="ACHGT5_000643"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN7216654.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="ACHGT5_000644"
     CDS             complement(13310..14236)
                     /locus_tag="ACHGT5_000644"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN7216655.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="ACHGT5_000645"
     CDS             complement(14249..15532)
                     /locus_tag="ACHGT5_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN7216656.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACHGT5_000646"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACHGT5_000646"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN7216657.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="ACHGT5_000647"
     CDS             complement(16320..17231)
                     /locus_tag="ACHGT5_000647"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN7216658.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACHGT5_000648"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACHGT5_000648"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN7216659.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="ACHGT5_000649"
     CDS             complement(17697..18395)
                     /locus_tag="ACHGT5_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN7216660.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="ACHGT5_000650"
     CDS             complement(18382..18768)
                     /locus_tag="ACHGT5_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216661.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="ACHGT5_000651"
     CDS             complement(18915..20585)
                     /locus_tag="ACHGT5_000651"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN7216662.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="ACHGT5_000652"
     CDS             20768..21001
                     /locus_tag="ACHGT5_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN7216663.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="ACHGT5_000653"
     CDS             complement(21100..21531)
                     /locus_tag="ACHGT5_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN7216664.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="ACHGT5_000654"
     CDS             21610..22023
                     /locus_tag="ACHGT5_000654"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN7216665.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="ACHGT5_000655"
     CDS             complement(22061..23209)
                     /locus_tag="ACHGT5_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN7216666.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="ACHGT5_000656"
     CDS             complement(23331..23927)
                     /locus_tag="ACHGT5_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN7216667.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACHGT5_000657"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACHGT5_000657"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN7216668.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="ACHGT5_000658"
     CDS             27157..27588
                     /locus_tag="ACHGT5_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216669.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="ACHGT5_000659"
     CDS             complement(27633..28982)
                     /locus_tag="ACHGT5_000659"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN7216670.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="ACHGT5_000660"
     CDS             complement(29052..30215)
                     /locus_tag="ACHGT5_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN7216671.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="ACHGT5_000661"
     CDS             30344..30667
                     /locus_tag="ACHGT5_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216672.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="ACHGT5_000662"
     CDS             complement(30850..32199)
                     /locus_tag="ACHGT5_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN7216673.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="ACHGT5_000663"
     CDS             complement(32268..33221)
                     /locus_tag="ACHGT5_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN7216674.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="ACHGT5_000664"
     CDS             complement(33323..34513)
                     /locus_tag="ACHGT5_000664"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN7216675.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34713..>34952)
                     /locus_tag="ACHGT5_000665"
     CDS             complement(34713..>34952)
                     /locus_tag="ACHGT5_000665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216676.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9948 a         7638 c         6119 g        11247 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaaggac
    34921 tgtgacaaaa gtcttgtcac agtcctattc tt
//
LOCUS       DAZBWR010000014        34703 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000014 DAZBWR010000000
VERSION     DAZBWR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.014"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            606..1295
                     /locus_tag="ACHGT5_000666"
     CDS             606..1295
                     /locus_tag="ACHGT5_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN7216677.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1282..2490
                     /locus_tag="ACHGT5_000667"
     CDS             1282..2490
                     /locus_tag="ACHGT5_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN7216678.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2784..4055
                     /gene="serS"
                     /locus_tag="ACHGT5_000668"
     CDS             2784..4055
                     /gene="serS"
                     /locus_tag="ACHGT5_000668"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN7216679.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4604..6088)
                     /gene="guaB"
                     /locus_tag="ACHGT5_000669"
     CDS             complement(4604..6088)
                     /gene="guaB"
                     /locus_tag="ACHGT5_000669"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN7216680.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6593..7288)
                     /locus_tag="ACHGT5_000670"
     CDS             complement(6593..7288)
                     /locus_tag="ACHGT5_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN7216681.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7378..8478)
                     /gene="ychF"
                     /locus_tag="ACHGT5_000671"
     CDS             complement(7378..8478)
                     /gene="ychF"
                     /locus_tag="ACHGT5_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN7216682.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8487..8678)
                     /locus_tag="ACHGT5_000672"
     CDS             complement(8487..8678)
                     /locus_tag="ACHGT5_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN7216683.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8699..9589)
                     /locus_tag="ACHGT5_000673"
     CDS             complement(8699..9589)
                     /locus_tag="ACHGT5_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN7216684.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9576..10343)
                     /locus_tag="ACHGT5_000674"
     CDS             complement(9576..10343)
                     /locus_tag="ACHGT5_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN7216685.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10355..11068)
                     /gene="rsmG"
                     /locus_tag="ACHGT5_000675"
     CDS             complement(10355..11068)
                     /gene="rsmG"
                     /locus_tag="ACHGT5_000675"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN7216686.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11300..13201)
                     /gene="mnmG"
                     /locus_tag="ACHGT5_000676"
     CDS             complement(11300..13201)
                     /gene="mnmG"
                     /locus_tag="ACHGT5_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN7216687.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13218..14615)
                     /gene="mnmE"
                     /locus_tag="ACHGT5_000677"
     CDS             complement(13218..14615)
                     /gene="mnmE"
                     /locus_tag="ACHGT5_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN7216688.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14914..15297
                     /locus_tag="ACHGT5_000678"
     CDS             14914..15297
                     /locus_tag="ACHGT5_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216689.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15465..16223)
                     /gene="jag"
                     /locus_tag="ACHGT5_000679"
     CDS             complement(15465..16223)
                     /gene="jag"
                     /locus_tag="ACHGT5_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN7216690.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16241..17059)
                     /locus_tag="ACHGT5_000680"
     CDS             complement(16241..17059)
                     /locus_tag="ACHGT5_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN7216691.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17056..17412)
                     /gene="rnpA"
                     /locus_tag="ACHGT5_000681"
     CDS             complement(17056..17412)
                     /gene="rnpA"
                     /locus_tag="ACHGT5_000681"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN7216692.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17530..17664)
                     /gene="rpmH"
                     /locus_tag="ACHGT5_000682"
     CDS             complement(17530..17664)
                     /gene="rpmH"
                     /locus_tag="ACHGT5_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN7216693.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18188..19522
                     /gene="dnaA"
                     /locus_tag="ACHGT5_000683"
     CDS             18188..19522
                     /gene="dnaA"
                     /locus_tag="ACHGT5_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN7216694.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19720..20850
                     /gene="dnaN"
                     /locus_tag="ACHGT5_000684"
     CDS             19720..20850
                     /gene="dnaN"
                     /locus_tag="ACHGT5_000684"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN7216695.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21079..21321
                     /gene="yaaA"
                     /locus_tag="ACHGT5_000685"
     CDS             21079..21321
                     /gene="yaaA"
                     /locus_tag="ACHGT5_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN7216696.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21308..22432
                     /gene="recF"
                     /locus_tag="ACHGT5_000686"
     CDS             21308..22432
                     /gene="recF"
                     /locus_tag="ACHGT5_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN7216697.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22429..24375
                     /gene="gyrB"
                     /locus_tag="ACHGT5_000687"
     CDS             22429..24375
                     /gene="gyrB"
                     /locus_tag="ACHGT5_000687"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN7216698.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24395..26866
                     /gene="gyrA"
                     /locus_tag="ACHGT5_000688"
     CDS             24395..26866
                     /gene="gyrA"
                     /locus_tag="ACHGT5_000688"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN7216699.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27102..27401
                     /gene="rpsF"
                     /locus_tag="ACHGT5_000689"
     CDS             27102..27401
                     /gene="rpsF"
                     /locus_tag="ACHGT5_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN7216700.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27451..27963
                     /gene="ssb"
                     /locus_tag="ACHGT5_000690"
     CDS             27451..27963
                     /gene="ssb"
                     /locus_tag="ACHGT5_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN7216701.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27989..28225
                     /gene="rpsR"
                     /locus_tag="ACHGT5_000691"
     CDS             27989..28225
                     /gene="rpsR"
                     /locus_tag="ACHGT5_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN7216702.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28368..30341
                     /locus_tag="ACHGT5_000692"
     CDS             28368..30341
                     /locus_tag="ACHGT5_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN7216703.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30347..30799
                     /gene="rplI"
                     /locus_tag="ACHGT5_000693"
     CDS             30347..30799
                     /gene="rplI"
                     /locus_tag="ACHGT5_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN7216704.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31073..32440
                     /gene="dnaB"
                     /locus_tag="ACHGT5_000694"
     CDS             31073..32440
                     /gene="dnaB"
                     /locus_tag="ACHGT5_000694"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN7216705.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33220..34512
                     /locus_tag="ACHGT5_000695"
     CDS             33220..34512
                     /locus_tag="ACHGT5_000695"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN7216706.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10567 a         6741 c         6863 g        10532 t
ORIGIN      
        1 aggtctactt catcaggagt gtttcccgat tcgtttttgg ctgccgtagc aacttttata
       61 tcatatcaga gagacaagta attgtcaact cttttttaaa agttttttcg atttcgtttt
      121 tcgaaatctt ccagcaactc atttatcata acttatcgtt tgttatttgt caagaacttt
      181 tttgaagttt ttttcgatag aatcaagctt cgcaagtggt tcttttttaa cgactcagtt
      241 atcttatcat gtcatttcca acatgtcaac aacttatttt ttattattta ataagttatt
      301 ggtgttcttt tgaacaaata ataatatatc aacatcacat gtgaaagtca ataagaaaat
      361 gcttatttat caacatttct agcttacatg atcattggtt agttctggtc ctttttcgtt
      421 cgtctttata actccgagag acgtaaaatc cttttgtttt cagtttaatg ttcatgttct
      481 tgtcttattc acaccaacta gatgtctcac cttctaaaat tttccttaac aaagtgctgc
      541 ttttatctat ttctgctttt ttttgctaga ataatagtaa caatatgact ctggaggatt
      601 tatttatgaa aattttgatt gctgacgatg ataaagaaat cgttgaatta ttaagtatct
      661 atgttcacaa tgaggggtat gaagcagtaa aggcttatga tgggaaagaa gcattatcta
      721 aaatcagaac aatccctgat attgaattac ttattttaga tatcatgatg cctgagatgg
      781 atgggatgca agtagtgaaa gagctacgaa aagaatccca aataccaatc attatgctga
      841 cagcaaaaac tactgatatg gataagatca aaggactggt cgctggtgca gatgattatg
      901 tgaccaagcc atttaatccg ttagaagtaa tggctagagt aaagtcttta ttacggcgta
      961 caaaaatgca gattgcccca gatcagcctg atgtattaga agtaaatgca ttaatcatca
     1021 ataaagactc tcatgaagtt aaaacaatcg atgggaaaga aatccagtta acagctttgg
     1081 aattcggtat cctctacttg cttgctagtc atcctaatcg agtatttagc gcggatgaaa
     1141 tttttgagcg tgtttggaaa caagaaagta ttgtctctgc taaaactgtc atggttcacg
     1201 ttagtcattt aagagataag attgaagaag caactggtgg tgaaaaagtc attcaaaccg
     1261 tttggggagt gggctataaa attgacgcaa gatagtaaac gagcaactga aaaacgccgt
     1321 cggatcaatt tgacttcaaa agaaatcagc gaattgttag cggaaggaat catcacgatt
     1381 atccttttgc ttctattaaa tgtttctatt ctggttgtta tcagttcggt gatcaacagt
     1441 tcaccttctt tgacgaatgc gatttgggat tcaaagaata tttttgcaga acgattgaac
     1501 actgatttgt tctggaacgg ccggaatttt attattccct ttttctttct attagatatt
     1561 ggtgttttgt attggcgctt gattcgaaga tatcgccaaa tgcaattgcg gcatatcatc
     1621 agcgaacttc actacatcgc aaacggaaat tatgatcatc gtatcccttt tgaactgagt
     1681 ggtgacttaa gccgagtggt aacaagtatc aatggattgg tggatagtac cgtagcagcg
     1741 attgaagacg aacgtaaaat cgaaaaatca aaagatgaat tgatcacgaa tgtcagtcat
     1801 gatatccgta ccccactcac gtccatcatt ggatacctag gactgataga agatggccag
     1861 tatcactcag aagaagatct tttaaagtat acgcatactg cttatatcaa agcaaagcag
     1921 atgaaatctt tagtggacga cttatttgaa tacactaagg tacgccagcc tgctgtcccg
     1981 gtgaattttt ctgcctttga catgatccag ttgatcgaac agttagcagc tgattttgag
     2041 ttggaagctt ctaaaaaaaa tatccaaata cttgttcaat caaaagtaga ctcccttatc
     2101 atggatggcg ataccgaaaa acttgtacgt gtcttcaata atcttttgac gaatgcgttg
     2161 aaatacggaa aaggagcaac gaagatcgta atcgaagtag aacgtatcgg ttcagaagtt
     2221 gttgcaacag tcaaaaataa cggagcaatg atcccacaac aggcaatcga taatctattc
     2281 gaccgttttt atcgtgtaga agaatctcgt tcacaagcaa ctggaggaac tggacttggt
     2341 ttagcaatcg ctcaaagcat tgtggctttg cacggcggat acatttatgc aaaatcagat
     2401 aagcaatgga cttctttcat catgcacctt ccaatcaaga aaaatgaaaa attacctatt
     2461 gatacacagg aagttattga cgaaagctga aatattcgct attcttatta caattattag
     2521 aataaatgaa aaacacagaa cccagtacac tttattcttt ttcagagagc tgatggcagg
     2581 tgtaaatcag caaaggataa tttggaagtt ccagttcgtt tacgtgccct ttcgaaataa
     2641 gaaaggtcgg ttcaacagcc gttatgtgtt caacaagtgc agctgacgct gaacttgggt
     2701 ggtaccgcga atttcagact gattcgtccc aagaataatg ggatggatca gtcttttttt
     2761 attttcaaaa aggaggaaat catatgttag atgtaaaaat gatgcgacaa aatattgaag
     2821 aagtacaaga aaaattgatg actcggggcg taaaaaaaga aacattagct gaattcatgg
     2881 aactagatga aaatcgtcgt aagctactag tcaaaagtga agaattaaaa aaatatcgaa
     2941 atgatgtttc tgctgagatc gcccaattga agcgtgcaaa agaagatgct tctgctaaaa
     3001 tcgctgaaat gaaagaagtc ggtggcaaca tcaaagcatt ggatactgaa attgctgaag
     3061 tggatgaaaa attgaaacaa attgcaacta ctttgccaaa ccttccccat gattctgtgc
     3121 cagttggaac agatgaagag gacaatgtag aaatccgtcg ttggagcgaa ccacgtacat
     3181 ttgcttttga accaaaacca cactgggaaa ttgcagaaaa tcttgatatt ttagattttg
     3241 aacgtggcgc aaaagtagcc ggtagccgtt ttgtctatta caaaggatta ggtgctcgat
     3301 tagaacgtgc tgtctacaac ttcatgcttg atcagcatat ttacgaacac ggctatactg
     3361 aaatgatcac tccttatatc gtcaacagta aagcgatgtt tggtacggga caatttccaa
     3421 aattcaaaga agatgttttc caattggaga atactgatct aacattaatc ccaacagctg
     3481 aagtaccatt aactaactat tataatgatg aaattttaga tggtgcagat ttacctattt
     3541 atttcactgc actaagccct gcttttcgtt ctgaagccgg aagtgctggt cgggatacac
     3601 gaggactgat tcgtcttcac caattcaata aagtagaaat ggtcaaattt tcggatgtag
     3661 agcattctta cgaagaattg gaaaaaatga cagctgatgc agaagatatc ttgaaaaaat
     3721 tgcatttgcc ttatcgtgtg atggccttat caactggaga catgggcttt tctgcagcta
     3781 aaacttatga cttagaagta tggatacctg cacaagatac ttatcgtgaa atcagttctt
     3841 gttctaactg tgaagatttc caagcacgcc gcgcaatgat ccgttatcgc gataatgatg
     3901 gaaaaatcca ctatactcat actctgaatg gttcaggact tgcagttggc cgtacagtag
     3961 ctgccatttt ggaaaactac caaaatgaag atggttctgt cacagttcca gaagcgctcg
     4021 ttccttatat ggggaatctg aaagttatta aataaagagg ctgtgaaaaa gccgcttagt
     4081 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaattggact
     4141 taataccgag aaactggctt ttgaacacca tttatcgaga ggttgtgaaa tcagcgaatt
     4201 aacctgagta ttagagaaaa aaatggaaaa atagtccctc atattttacc gtttgttgaa
     4261 ctaataccga gggtcagctg attgaacacc gtttagtgga gaggtcgtaa gcctaccccc
     4321 cagctcctag ttacaaggac aaattcaaaa aacagctctt catattttat gaatgttgta
     4381 cttgtgattc ggaggtggca agggaacact gtttattcaa aaagaggctg ggacagaagc
     4441 gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
     4501 tcttcttatt tccgaaggag ttgcttctac ttccgccgtt tatacgtgtt tagagcgtga
     4561 gacaaaagtg ttctttactt ttgtcccacg ctctttaatg tttttaagat tcaactgaat
     4621 agtttggtgc ttcttttgtg atttgtacat catgagggtg tgattctttt aatccactgc
     4681 cgctcatttg gataaattgc gcttcatcac gtaattgctg tagattggct gccccaacat
     4741 aacccattcc tgaacgcaaa ccgccgatca tttggaaaat aatatctgca acacttcctt
     4801 tataagcaac ccgaccttcg attccttctg gtactaattt atttgcttca ttgacactac
     4861 cttggaagta acggtctttt gaaccttttt ccatcgcgcc taatgagccc atacctcggt
     4921 atgttttgaa tcgacgtcct tggaagattt cgaattcacc tggtgattca tctgtccctg
     4981 ccaacatact tcctagcatc actgcatgtc cgccggcagc taatgctttg acgatgtcac
     5041 cagaatattt gatccctccg tctgcaataa tcgctttgcc atattgacga gcaactgatg
     5101 ctgcatcata aatagctgtg agctgaggta cacctacacc ggctaccaca cgtgtcgtac
     5161 aaatcgatcc tggtccgata ccaactttta cgacatctac acctgcatca tataaagctt
     5221 ttgtcgcttc agccgtcgca acgtttcctg caatcaatgt tgcatctgca aaagttgaac
     5281 ggatttcttg aatttttcgg atcacacctg cactatgtcc atgagctgta tcgatgatga
     5341 ttgcatctgc ccctgcttct aataaagcat tcgcacgctc aaatgtatcg cttgttacac
     5401 caacggctgc tgctacaagc aatcgaccat gttcatcttt tgctgcattt ggaaattcga
     5461 tcactttctc gatgtctttg atcgtgatca atccgcttaa acgaccttcg ttatctacga
     5521 ttggtaattt ttcgatttta tgtttttgca aaatcttttc tgcatctttt agagaggtac
     5581 ctactggggc agtcactaag ttgtcttttg tcatcacatc agcaatcggc atgtgataat
     5641 ctgttacaaa acgcatgtcg cggtttgtaa ttatccctac caattttctg ttttcaagtg
     5701 tttcaacgat tggcacacca ctgatacgat atttgctcat caattcttct gcgtctgcta
     5761 caagatgctg cggcgttaag aagaacggat caatgattac gccactttca gaacgtttga
     5821 cttttcttac ttcatccgct tgctggctga tcgtcatgtt tttatggatc acacctagtc
     5881 cgccttgtct tgccatagca atggccatct tgctgtcggt aacagtatcc atacttgcac
     5941 tgatgattgg aatattcaac ttaatatttt tagctaattg tacgctcata tccacttcgt
     6001 ttggtaacac gtgactttct gctggtatca ataagacatc atcaaaagta aatccttttt
     6061 tcgtgaactt tgtttcccag ttggacattt ttagtaccac tcctctaatt tagtttatct
     6121 attaaaataa ttgatttttt catttaagtc aatgatctac ttggaatcaa gttagctttc
     6181 tcatagaata ccaatataaa gcgtttacat ctgtgtgtat taaaagagat tgtaaaaaag
     6241 ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg tttttgaatt
     6301 ggttgggttt attaccgcag atgcaaactt ttgaacaccg tttagtggag aggctgtgaa
     6361 aaagctgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc cacatttttg
     6421 cataaattgg acttaatacc gagaaactgg cttttgaaca ccgtttatcg taaaaaggga
     6481 ctgtgacaag acttttgtca cagtccctta ctattattta actatggata aacaaaatga
     6541 ggttctttac tacccctaga tatctagcaa aatagctgat ctttttgcta gtttatcttc
     6601 ttgggacgga taaacttcca ataatcccgt attttttctt caagtagtaa cggattgcta
     6661 aagcagttgc cccgataatg ataactacta ctggatctaa tactggattg ataactggtg
     6721 gcaatagtgt tgtcgcagaa aacagtacga cccaaagaag tgtcgttcca cctaggataa
     6781 gcaatgtctt acccattcct ggtttcttgc ttcgatctgc tccgggatat tcatattgat
     6841 agatgtattt gtacatcata taaaagaccc agccaccgat tgcagaacct agaacgagtg
     6901 tagaaaagcc ataagctggc gttgttccac gtgaaaacaa tccggtaatc gcaatcatga
     6961 tacttaatag accaagcaac agcaacgtat tatctaacca catcaataaa ggggtggatg
     7021 aattttttac ttctgctggc ttattgatga ttgcttccgt tctttcagag actgtcccaa
     7081 acaattgacg ggctgttctt ccgccttttt gttctgcaac taaagtaggc agaatgtcat
     7141 gaagggcttt tactttttct tcttcagaca aattcgctgc ttctaaagat tttttcaaat
     7201 caaaaatgta ctgttgattt cttttggtta attttgtttc taaactattg ttttctttta
     7261 cgattgctcg taattgttct ggttccatta tctaatagtc ctccttagcc gttccttttc
     7321 tcaacggtcg tcacaaaaga cattctacca tattctacag ctagtttgat agccctttta
     7381 cacattaaag cggaataaca ttacgtcgcc gtcttgtacg acataatctt ttccttctaa
     7441 tcgaacacgt ccagcttctt ttgctgcatg catgtttccg taatgatcca agtcttcaaa
     7501 agagacagtt tctgcacgga taaatccgcg ttcgaaatct gaatggatga ttcctgctgc
     7561 ttgtggtgct ttgattcctt ttttgaatgt ccaagcacgt acttcttgtt ccccagcagt
     7621 aaagtaagta gctaagccta acaaatcata agcagcacgg atcaactgat ccaaacctga
     7681 ttcttcgatt cctaaagctt ctaaaaattc cgctttgtct tcttcatcta attctgcgat
     7741 ttcttcttct gcacgagcac tgaccacgat cacttctgca ttttcttctg cagcaaagtt
     7801 gcgcacttct tgtacataat gattcgcgtc tgcatctgct acgtcatctt ctgagacatt
     7861 ggctacataa agaattggtt ttgtcgtcaa taaaaatagt gatttaacga ttttttgttc
     7921 ttcttcagta aattcgatcg ttcgggcaga ttttccttct tccagtacag gtttgatttt
     7981 atccagtaca gctagttctg ccactgcatc tttatctttg gttttagcaa cttttgctac
     8041 acgagtatag cgtttgttga tagattctaa gtctgctaac actagttcca agttgatcgt
     8101 atcaatatct tctagtggat caacacgtcc ttctacgtga gtaatattat catcatcaaa
     8161 acagcgaaca acgtgacaaa tagcgtctac ttggcggata tggcttaaga attgatttcc
     8221 caaaccttct cctttacttg cgccttttac gattccagca atatctgtaa attcaaaagt
     8281 cgtcggaaca gtttttttcg gatgtacaag ttctgttaag cgttgcaaac gccagtcagg
     8341 tacttctacc atccctacgt tggggtcgat cgtcgcaaag ggatagtttg ctgcttctgc
     8401 tcctgctttt gtgatcgcat taaacaaagt tgattttcct acgttgggca acccaacaat
     8461 tccagcagtc aatgccataa atttattcac tctttctctg catttttttc aattactttt
     8521 tttaatttct tttcaaaatc tcgacgggtc atcatgacga tatgtccgca attcatacat
     8581 ctgattttga tatctgcgcc cattcgaatg acttcccagc gattcgtctg gcaagcgtga
     8641 ggttttttca tttcaacgat atcgtgcaag tcgtacataa aaatagcctc ctttttgttt
     8701 attcttcatc aaattgaata ttcaagatat caagaattcg agtcaaatct ttttgagaaa
     8761 gatattcaat ctcgatctta cctttcccat ttttttcttg gatagctaca cttgtaccaa
     8821 atttatccat caaacgatct tcgctttctc ggatataata tggtttttcc tttgctagcc
     8881 ttgggatttt tttcttttct tttccatcat tttgatttaa ctcagaaaca agctgttcca
     8941 gttggcggac agttaaattt tcagttactg cacgattggc taatttcaag atcaatgttt
     9001 tattttttaa acctaacagc gtacgggctt gtcccataga taaacgctga tcttgtacca
     9061 tttccttgac aagatccggt aaagaaagca agcgcaaata attggcgata taaggtctgc
     9121 tctttcctaa acgactagct acttctgctt gtgttagttt caggtttttc atcagcatct
     9181 catatgcttc tgcttcttct aacggattca gatcttctcg ctgcaagttt tccagcactg
     9241 ctacttgcat catcgcttct tcgtcaaagt ctctaacgat agcagggatc gtttcttttt
     9301 cagctagttt ggacgctcga aaacgtcgct cccctgcaat aatctcatat ccttttactg
     9361 cggaacggcg tacgatgatg ggttgaaaga cacctgattg ttcaatagaa ttagctagtt
     9421 cctgcaaaga tacttcttca aatgttttac ggggctgata cggatttggt cgcagctcat
     9481 tcaatggtag ctggacaacg gtatcatctt ttacatccac cgtctctaag ttttctaaat
     9541 cttgaaacag tgcatcgatt cctcggccta atcctttact tttcttcacg acttaccact
     9601 tcctttgcca atgcttggta gacttcagct cctcgagaac gtggatcgta gtcgatgatc
     9661 ggttttccat gactaggtgc ttctgataaa cgaatatttc ttggaatgat cgtttcgtaa
     9721 actttttcgc ggaagtattt acgtacttct tctacgactt catttcccag atttgttcgt
     9781 gcatcataca ttgtcagtaa gactccctcg atctctaatt ctggattaaa gtgtttttgg
     9841 actaaacgaa ccgtattcag taattggctt aatccttcca atgcgtaata ctcacactga
     9901 acaggtatca aaatcgaatc acttgctgta aatgaattga ttgtcagatg gccaagagaa
     9961 ggcgggcaat cgatcaaaat ataatcatac tgactgctta cttctgctaa agatcctttt
    10021 aatcttgatt ctcttgccat cattgacgtc aattcgatct ctgctcctgc taattggaga
    10081 gtcgcaggca caatactcaa attttcgtgt tcagtgatca aagttgcctc atcaattggc
    10141 aattcattga ctagaacatc atagatgtct cttgtcacat caggttttct gatcccgaca
    10201 ccactggtag catttccttg tgcatccata tccactagca atactttttt gccaaggcta
    10261 gcaagacaag cacctagatt caccgtcgtc gttgttttgc cgacgccacc tttttggttc
    10321 gcaacagata ttatttgtgc catctcttct cctcctattt gattggctgc ttattaggca
    10381 gtccgggctt tcttgggtat ttctttgggg ttggtttctt tttttgaata acgatgatat
    10441 gccgttgatc agagacattc ggcaaggtaa ccgcctcttc tttgaggaat ttaccaccta
    10501 ataaagcaat cgcttgtttt gcttctgtca attcctcttc actttttgct gctttcaatg
    10561 cataaaaata gccttcttgt ttagccaagg gcaaacacaa ttccgctaac acattcaaac
    10621 gagcaaccgc acgagcagtg acaaggtcaa atgatgcacg aaagtgagga ttttgcccaa
    10681 atgtttctgc ccggtcatga tataaggaag catcgattcc taataatcgg gttaactcag
    10741 ataagaaggt tattcgttta ttcagagaat caacgatcgt tatctgcagt tctggaaagg
    10801 caatttttaa aggtatgcta ggaaaccctg cccccgaacc aacatcgcat aaagataaag
    10861 gttgcgtaaa gtctacttca aatgctaatg taagtgaatc gtaaaaatgc ttcagatata
    10921 cgtctttttc atccgtaata gctgtaaggt tcatcttttc gttccattcg actaatgtcc
    10981 gataataaat agcgaattga ttcatttgat gatcggtaag ctctatccct ttttctgcca
    11041 aggattggcg aaattcttct ggtatcataa atattccttt cattgtgaaa cagcaattgt
    11101 ttcacagatt ctctccctac tttaacgcaa aatctctaga catgcaatcc ttccatatgg
    11161 attcttaaaa atttgaaaag taagaaaagc cgatagactt tttctttcta gtatgactca
    11221 taaaatatac acagaagaga gaactagctt tgagcaattt ctgcaaaaac tagttctctc
    11281 ttctatttat tattacgtat tacgctggtt cactgacttt cgcaatcttc ccttgttcga
    11341 tatagaccat taggatactt aggtctgcag ggtttacgcc actgattcgg ctagcttggg
    11401 caattgtttc tggttggatt ttctgcaatt tttgttttgc ttctgttgct aatccgttga
    11461 ttgctgcgta gtcgatgttt tctggaatct ttttcgcttc catgcgtttt aatttttcta
    11521 ctttttccat ggcttttttg atgtagcctt catatttgat ttggatttct acttgttcga
    11581 tttcttttcg tgtcaattct tcattttctg gaataaaccg tagaagttct gcataagtaa
    11641 tctcaggacg tcttaaaaag tcgcttgcta gtacaccatc ttttaatggt gccgcattaa
    11701 tcgttgctaa aaagtcttgg acttcttctg ttggtttgat tcgtacattc atcaaacgtt
    11761 tgatttcttg ttcaactgct tcttttttct tcaaataatt cttgtattga tcttctttta
    11821 ctaagcctac acggtgaccc atttctgtta atcgcaaatc tgcattatca tgacgtaaaa
    11881 gcagacggta ttcagcacgt gaagtcagca aacgataagg ttcgttcgtt ccttttgtta
    11941 ccaaatcatc gatcatgaca ccgatgtaac cgtcacttcg tttgatcact aaaggttctt
    12001 ttccctgaac tttcaaagct gcattgatac cggcaatcag cccttgtcca gccgcttctt
    12061 cataaccgct tgtaccattt gtttgtccag ctgtataaag attttcaacg actttagttt
    12121 ctaatgtcgg acggagctgg tgtggctcta ctacgtcata ttcgatagca tatcctgtac
    12181 gcatcatttc tactttttct aatccagcaa tagaatgaag catgttgact tgaacatcct
    12241 ctggtaaaga agtagataaa ccttgaacat agacttcttc cgtatttaag ccttctggtt
    12301 ccaaaaataa ttgatgacgt ggtttgtctg caaaacgcac gatcttatct tcgatggatg
    12361 gacaatagcg agcgcctacc ccttcaacga tccctgtaaa cattggggca cgatgtaagt
    12421 tgtctcgaat aatttggtga gtcgtctcac ctgtataggt cagccagcaa gattcctgat
    12481 ctagtttata ggcactatct ggtgtgctga agctaaaatg attcggctct ttgtcaccag
    12541 gctgctcttc tgtaacagaa tagtcgatcg tacttgattt tactcgaggc ggtgttcctg
    12601 ttttgaaccg ttcgatagca aagccaagtt cttttagatg attcgctagt ccaatagaag
    12661 gttgagagtt attcggacct gatgaatatt tcaattcacc gataatgatt tctccgcgaa
    12721 gtgctgttcc ggctgtaata acaactgctt tggcccggta gctcgctcct gtggacgtca
    12781 ctactccatg acaaacacca tcttcgacga tcagttcttc gacgattcct tgtcttagcg
    12841 tcaaatttgg ttctttttcg atcgtatgtt tcatttctgt tgaataagca tgtttgtctg
    12901 cttgtgctcg caatgctcgg acagctgggc cttttcctgt gttcaacata cgcatttgga
    12961 tataggtttt atcgatattc ttacccattt ctccgcctaa cgcatcgatt tctcgaacga
    13021 caacgccttt agctggtccg cctacagaag gattacatgg catgaatgct accatgtcca
    13081 aatttattgt cagtaataat gtttttgatc ccatacgtgc agcagcaagt gcagcttctg
    13141 aaccggcgtg tcctgcgcca acaacgatca cgtcatacgc atctgcttca taatgttcca
    13201 atcgtttgtt cctcctttta ttttcctaaa caaaattgac tgaataattg agtgattagc
    13261 tcatcttgta cactgtcgcc gacaacttct cctagatagt cccagcaacg cgtcatatct
    13321 atttgaacga gatccacagg cattcctgaa tcaatacctg caatgacttc ttcaagtgag
    13381 agggaagctt tttctaacaa cgcgatatgg cgggtattag aaacataagt agcgtctctt
    13441 tctcctgttt ctccgctgaa gaataaatca gaaatagctg attccaaacg atctagtccg
    13501 tcgtttttag ctacggaaat tgaaaaaact ggttcatttt cgatcaattt cttcaatttt
    13561 tcttgttcca gctgtgctgg caaatctgtt ttatttagaa gaatgatccg tttcaatcca
    13621 cttgtcgctt ccaataattg ttcatcttct gctgtcagtg gttcgctttg gtttagaaca
    13681 agtaagatca agtctgcttc tgctaatgct tttcggcttc tttcgacccc gatccgttcc
    13741 accacatctt ctgtttcccg aattccagct gtatcgatta gcttgagcgg taccccccga
    13801 acattgacat actcttcgat tacatctcga gtcgttcctg caatatccgt aacgattgct
    13861 ttttcttctc ttaacaaatg gtttaacaaa cttgatttgc cgacatttgg ccggccgatg
    13921 attgccgtac ttaacccttc ccgcagaacc ttcccttgct tggatgtagc aagtagcgct
    13981 tggattcttt gttggatcat ctgcgctttt tcaagcaata acttcgtagt caattcttcg
    14041 acatcatcat attctggata atcaatattt acttctactt gtgctaatgt ctctaaaatc
    14101 tcttgtctca atgagcggat caaagcagac agattgccgt ctaactgatt aagagctagt
    14161 cccattgctt tgtctgtttt tgcccgaatc aaatccatca cagcttctgc ctgtgaaagg
    14221 tcaacacgac cattcaagaa tgctcgtttt gtgaattcgc ctggctcagc taatcttgca
    14281 ccttctctta acaatagctg caaaatttga ttgactacaa cgatcccacc gtgacaattg
    14341 atttctacta catcttcccg cgtaaatgtc tttggtgctc tcatcacgga gaccatgact
    14401 tcatcgacaa gctgattgct cttgggatca acgatatgtc cataatggat cgtatgactt
    14461 ggtacttctg ataatcgttt gtttccagat tgataaactt tatctgctag ttgcacagct
    14521 tgatcgccac ttaaacgaac gatactgatt gcaccttctc caggtggggt agaaattgcc
    14581 gcaatcgtat caaattctaa agtaatttca gccatttttt cacttccttt ttcattttgc
    14641 gcacaaaaaa agtgcccact ccacccttta tgcacaataa acggtgaact tcgcactttg
    14701 actgcttgtt cttgattaaa ttctggtcat agattatcac acttgctata aggaatcaag
    14761 tgtttaaaac aattagatga agttgagcct gttctgcttc cattacctta tatttaattg
    14821 tccttggatg tctctttgcg ttctttggtt tattcgaaga gaagatactg tataaacaag
    14881 gctttatctt tcttaaaaaa aacggtacac taaatggtag cgctatctaa actatacact
    14941 tttgaaagga gaaaatatgt gagcccgttc agatctgctg cattggaaga agaaagtgac
    15001 cctcggatca acgatgtcgt tttatctaca gggaatatca accagaaata tatcgtccgc
    15061 gatatcgttt ttgttgtgga atgcttagaa gctgatttgt ttgacccatc ttttcccaaa
    15121 gatgaccttt tcttaacaac gaagaggaaa ttaaagacga aggtacttga atatggtgcg
    15181 aatgcagtaa tcaactgtca ttttgagtat cagcaaaaaa aacgcgacgg aaagtatttt
    15241 ctggaacttt ttgcttatgg aacagtgatc caatttatcc aaacaacgat tggttaaatg
    15301 aaacagacaa aaaaagcgga tcaaaactga agacagtttc gatccgcttt tttctatcaa
    15361 agttagtgac tacttcatcg tatactctga taagaatgat ttctagtaaa atatacttgg
    15421 tttgaaaaac tttgtcaacg tgatttattc aaaaagtaat ggaatcaata atatttttta
    15481 gcaggttcta ctactaaata acgataaggt tcctcacctt ccgaatgtgt ttttacatgt
    15541 tcatctttgc ttaatgcagc atggatttgt ttacgttcaa atgcaggcat tggttctaag
    15601 aaaacaggct gccctgtccg ttttactttt tcagccgtgc gatcagctaa acgctgaatg
    15661 atctcttggc gcttttcacg gtaattgcct acattgacta ctacagacag cttatttctt
    15721 gctaaacgat ggatgaagac ttgtgccaga tattgcaatg cattcagtgt ttttccatgt
    15781 ttcccaatca gtatgccttg cttttgtgtt tctaaatgga aaacaaccat attatttgat
    15841 tctctttcca ttttgacaag agctggagca tcaagctgtt ttgaaatttc agtaagataa
    15901 actgccaatt ccgttaaagc tgcttcatca cctagctcac ttacttcttc tgttttttct
    15961 tcaagaatcg tttcgttgac tacctcgatc tcagctgttg gttcttcttc caaaatttct
    16021 tctacagtct gttctactgt ttcagaaact ttttcgctga tacttggctg gatcgaaagc
    16081 cgtgcattct ttcgacccat tcctaggaaa cctttttttc cttcttccag tatttcgatt
    16141 tcgacttggt ctttggccaa acccaaatca gccagaccta gttggacagc ttcgtctatt
    16201 gttgcagctt cataaatcgg cattagcatt acctccccta ttatttccgt tttttcttag
    16261 gattcattgc tttcttaaga gcacgttctc tttcgcggat tctccgtgct tcttcttcac
    16321 gctcttttcg aattttgaaa gggttgttga tcagcagcgt ttgtacaact tggaaggcat
    16381 tggaaactac ccagtataat gaaagaccgc tggctaaatt gacacccatc actaaaatca
    16441 ttacaggcat cacgaaattc atgatcttca ttgatgcatt cgattcaatt tgactcatgc
    16501 tagataaata cgtacttgca aaagtaaaga ttgctgctaa aatcggcaaa ataaatgttg
    16561 gatccgcgtt ccctaagttc aaccataaga aatgcccttg tttcaactct ggtacacgtg
    16621 aaatagattg ccataacgcc atcaagattg gcatttgtac taataaagga agacaaccag
    16681 catatggatt gacgccattt tctgcatata aacgctgctg ctcttctctt aacttacttt
    16741 gtgtttccgg atctcttgaa ctatattgtt gctgcagtgc ttttaatttc ggttgtaatt
    16801 cttgcgtttt acgcatgctc tttgtttgga aatgcatcaa cggcaatagg atcacgcgga
    16861 tgatcagtgt aaataaaatg atccctatcc ctgcattacc aaatgacaac gccttgatcg
    16921 cctcagcaaa gtaataaaca atataacgat cccaaattcc tgtactctgt gcgctgactt
    16981 ccccagttcc gcaaccggat aagacgatga ccaaactaac gataccggcc atcaaaagta
    17041 aacgtttgta tttcttcact tactcttttc ctctctgtcg attatttttg caagttttaa
    17101 gacgtggact aaattggagc gaacctcatc ataagtcaac cccttcacac ttggtctagc
    17161 aatcacaata aaatccaact ccggatcaat ttcttctttt atagaataaa ctgctgctcg
    17221 gatttttcgt ttgacttcat tgcggtttac cgcgttgccg accttttttc ctacagataa
    17281 accaactcga aaatgaggct gtcccggttt ttccaaacgg taaacgacga atttgcggtt
    17341 agcaaaagaa tctcccgtat ggaacacggc ttgaaactct cgttcttttt ttactcgata
    17401 cgcttttctc attagttttc tttccttttc tatctctcac ttacgctcat aatcatacca
    17461 taattacgat tgtcctttca tcatttccct aaaaaagagt ataaaaaaaa ccactgaaac
    17521 cccagtggcc taagctgaaa gaacttttct tcctttgcga cgacggctag ctaacacacg
    17581 acgtccattt ttagtactca tacgtttacg gaatccgtga actttttgac gtttacgttt
    17641 atttggttgg tatgttcttt tcatatattc cacctccaaa atgttcttat ctattttata
    17701 aacacagaca tactttgata gtataacgac aaagtagtga ttttgtcaat ataaaataga
    17761 agatcgattg ttaaaactta tccacaagat tatttttttc tatgggagtc ctatgtggaa
    17821 aaagttatgc acagggatta tccacagata ataaatggct ggttttacac agaaaacaca
    17881 cgtgtaaaaa aagtaatcca caatcaggtg atttcctgtg tattccgtct cctaactatt
    17941 ggtatatcta tattttttat ccacagatgc aagaggaaat ctattgattt ttacgagttt
    18001 tccacctgtg cattagtggt gtggaaaact tttttttgac aactgtgttt ttctcttttt
    18061 tctttttgtc gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg
    18121 aaaactattg aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag
    18181 gccttatatg gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga
    18241 tttatcgcct gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca
    18301 aaaccagctc gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct
    18361 ggcgacaaaa atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt
    18421 tattactggc gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt
    18481 ggcacctagt ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt
    18541 cattggtaaa ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg
    18601 ttctatttat aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat
    18661 gcatgcaatt ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag
    18721 cagtgaaact tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt
    18781 ccgtcaagaa tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga
    18841 aaaagaagct acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa
    18901 acaaatcgta ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct
    18961 cgtttctcgc tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag
    19021 aacagcgatt ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact
    19081 aagctacatc gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg
    19141 agtgcaagct tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc
    19201 actgaaaacg ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga
    19261 agaagtggca aagtattatc acatccaatt gaaagatatc aaaggaaaga aacgcgtcaa
    19321 atctattgtt gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc
    19381 tttaccgaaa atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca
    19441 tgaaaaaatc cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat
    19501 caaaaacttt ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa
    19561 aagaaaaaaa cgttcaaaag ttatgcacac tttttccaca gctgtttttt cgtttctctc
    19621 ttacgttttt tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta
    19681 actaataata aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga
    19741 gctagcttta tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc
    19801 cctattttga caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc
    19861 aacgctgata tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa
    19921 atcgaatcta ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga
    19981 cttcctgaag aaacatttac tttagaagtt ttagaaaata aacaagtagc gattacttct
    20041 ggaaaagcga attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt
    20101 gtcgaaagcc ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa
    20161 acagtttttg ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt
    20221 ttatctgata attctttatt ggctgtagct actgattctc accgtctaag tcaacgcgtg
    20281 attccagtag aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc
    20341 gaattatctc gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac
    20401 caagtgttgt tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat
    20461 cctgatacca atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct
    20521 agctttttag cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc
    20581 gttcgtttat ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg
    20641 aaagtagaag aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc
    20701 aatccggatt atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa
    20761 tttatctctg ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt
    20821 cagctgatta ctcctgtacg tacaaattaa caatgatttt cattgttctt gaaaatttaa
    20881 ttgaaaagaa atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc
    20941 tttctgttta tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat
    21001 atatggataa atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat
    21061 gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg
    21121 ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg
    21181 gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat
    21241 cccggcatga tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac
    21301 gacgataatg aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa
    21361 catcgaattt tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa
    21421 tcttttggaa agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca
    21481 ggaattgatt ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc
    21541 atccactctt ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca
    21601 tatcgaacaa aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc
    21661 agaagatctt tctttagtaa aaggcagccc gcagatcagg cggaagtttt tagatatgga
    21721 attaggccag atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa
    21781 acaaagaaac cagtatttaa agcaattgaa tgaaaaaaaa caaactgatt tgctttattt
    21841 ggatattttg tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca
    21901 gttcgtcaag cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa
    21961 agagcagctg gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga
    22021 gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg
    22081 gggaacaacg acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa
    22141 tgtgcagact tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc
    22201 agaaatcgat ttgattaaag aagagacggg ggaatatcca atccttttgt tagatgatgt
    22261 gatgagcgag ctggatgata gtcgtcaact ccatctgcta gaaacaatcg aaggaaaagt
    22321 tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc
    22381 tgagattttt tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa
    22441 agaagtttag tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga
    22501 cttgaagccg ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg
    22561 catcaccttg tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca
    22621 acgaaaatcc atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg
    22681 gggatacctg tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact
    22741 gttttacatg ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac
    22801 ggagtaggat cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa
    22861 gaaggaaatg tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt
    22921 atcgaacaaa cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt
    22981 acagagacaa ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc
    23041 ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat
    23101 gaataccatt atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc
    23161 gtgatttttc cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc
    23221 tctatgcaat atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat
    23281 acgtatgaag gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat
    23341 gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac
    23401 gtgcgagaag gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga
    23461 caaacaaaaa caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc
    23521 gaacatttca tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa
    23581 ggattactag cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt
    23641 aaaggtgctt tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat
    23701 cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa
    23761 ggtcgtaatc gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa
    23821 aaagcaagca tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg
    23881 ggtacagggt ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc
    23941 atgactgatg ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat
    24001 cgttacatgc gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac
    24061 ggggtaaaac aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag
    24121 ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa
    24181 ggacttggag aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg
    24241 atgatggccc gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg
    24301 atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag
    24361 aacttagata tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat
    24421 ccatgatgtc aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt
    24481 tattgtcgca cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat
    24541 tttatacggc atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag
    24601 aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc
    24661 aatggtacgg atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa
    24721 ctttggttct gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag
    24781 caaaatcgct acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa
    24841 ctatgatgat acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt
    24901 gaacggaaca acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc
    24961 tgaggtagta gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt
    25021 gatggaagta ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg
    25081 aatccgaaga gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat
    25141 tactgagatg ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa
    25201 taaagcaaaa ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat
    25261 cacagatttg cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg
    25321 tgacgccagc gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc
    25381 atttggattc aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca
    25441 aattcttgaa aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga
    25501 aaaagaaaaa gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca
    25561 tatcgatgaa atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac
    25621 gatgatcgaa cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt
    25681 acgccgattg accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa
    25741 actgattgaa gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa
    25801 gaatgaatta gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt
    25861 cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac
    25921 attgacaaac aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag
    25981 gggtggacgt ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt
    26041 ttcttgttct acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc
    26101 aaaaggatac gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt
    26161 actaggtatc gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga
    26221 agaaggccac tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac
    26281 tgcttttgct aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga
    26341 gctcgtcaat gtgctactga cagatggtca atctaatatc attattggaa cacataacgg
    26401 ctactctgtc actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt
    26461 tagaggaata cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga
    26521 tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata
    26581 tccagtgaaa ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg
    26641 tccactagct ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa
    26701 aggtgtcatt atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg
    26761 cgtacgtttg atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc
    26821 agaagctgca gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat
    26881 tgtgtaagct agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga
    26941 ttagttgaat ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa
    27001 tagagtgtat aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt
    27061 aggaacctaa gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg
    27121 aaatcatgta cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac
    27181 gtttcgatgc aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa
    27241 aacgccgttt cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag
    27301 tatcttctcc atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg
    27361 acgatatcat tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac
    27421 aatagaaaac gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt
    27481 ctgacgaaag atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact
    27541 ttggctgtga atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac
    27601 tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt
    27661 ttaggtgtta ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta
    27721 tacgtgacag aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa
    27781 aatcgccaac aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga
    27841 aacaataact ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat
    27901 acttctgacc cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc
    27961 taaacgaatt tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg
    28021 tcgtaaagta gactacattg ctgctaacca tattgaatat atcgattata aagatatcga
    28081 attactaaaa agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac
    28141 aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt
    28201 gctacctttc gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat
    28261 cagctattca attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg
    28321 acgaaagtta tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa
    28381 acaatcattt atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg
    28441 tagtcttaat tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat
    28501 ggggtgttcg ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa
    28561 tccagcaggc ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg
    28621 taggagtgat tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat
    28681 tgaaaatcaa agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg
    28741 caaaagaaaa aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg
    28801 atacagtaaa gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc
    28861 atcagacgaa cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca
    28921 ttgataaatt ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt
    28981 ctgactggat gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct
    29041 tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc
    29101 gctttcgagc agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt
    29161 atggctcaag aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt
    29221 tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt
    29281 attatggggg gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga
    29341 gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt
    29401 ttccggatat ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc
    29461 agaaaaaagc ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc
    29521 ttgaagaaat ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca
    29581 tagagctgaa aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat
    29641 cgatctctca gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc
    29701 gtggggaaga gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag
    29761 cttcagagct agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc
    29821 gttttgaggc cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc
    29881 gtacaactgc acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat
    29941 ccttagtaca atatttattg agctcggatt tagcgacata cttggagatc agcaatttga
    30001 ttgctggaag cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag
    30061 agtataatag tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca
    30121 atgcagcttt cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta
    30181 ccggcacgat caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa
    30241 acgcagctac tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg
    30301 caatcaaaga aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt
    30361 tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta
    30421 tgcacaaaat tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag
    30481 tcagttagca ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc
    30541 taaaaagtta aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc
    30601 aggggaagac agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa
    30661 caaacaatac aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc
    30721 actaggctac acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt
    30781 tcatgtcgta caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga
    30841 aggaaaaaag acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc
    30901 tgcaaatcga tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag
    30961 aaaacgagta taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact
    31021 tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga
    31081 agtgtggcag gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc
    31141 tgtttttctt gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt
    31201 ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga
    31261 agcgattgac gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc
    31321 cggtggttta agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt
    31381 ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac
    31441 aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc
    31501 cgaacgcagt atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc
    31561 tgaagtactg aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat
    31621 cacaggtcta ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga
    31681 agaactaatc attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat
    31741 tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg
    31801 ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt
    31861 acgtacaggt cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct
    31921 ttctcgagca aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc
    31981 gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt
    32041 gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg
    32101 ccaactgaaa aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc
    32161 tcgtggagta gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg
    32221 atcaattgaa caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag
    32281 agaaggcgga gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat
    32341 catcgagaag aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata
    32401 caataagttt tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg
    32461 agcaatcccg agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta
    32521 cttaatttaa acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc
    32581 cgaataaacg gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg
    32641 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct
    32701 cttttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa
    32761 catgagaagc agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca
    32821 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga
    32881 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    32941 cacaacctct gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc
    33001 acaaaaattt gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc
    33061 tgttacaacc tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt
    33121 ttataaaata aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac
    33181 aatggttcag gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta
    33241 ggtacgcaat ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct
    33301 gaagtgattg cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt
    33361 gtgacttata aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg
    33421 atcggaaacg gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat
    33481 gaaaaaggtg tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct
    33541 tatcatatca aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca
    33601 acgattaaag gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt
    33661 gcagacttat tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa
    33721 aaccgtcaat ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa
    33781 gaatattatg aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg
    33841 aatgatgctt tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg
    33901 gatatcgacc agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta
    33961 acaatcggaa gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc
    34021 tatacgtcac gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa
    34081 caaatccgcg aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc
    34141 tggtttgatt ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct
    34201 ttaaactcga tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa
    34261 ttagatggcg aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa
    34321 ccaatctatg aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca
    34381 gaactgccag aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt
    34441 atctcaacat tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg
    34501 gcgcaaatct aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga
    34561 agtgtttatc tatacgataa taataacttg aagaggtagt gaaagagacg tttagcctta
    34621 ttgccgatag gctgtctctt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    34681 gtgtcacagt ccctttttcc gaa
//
LOCUS       DAZBWR010000015        34643 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000015 DAZBWR010000000
VERSION     DAZBWR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.015"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..837)
                     /locus_tag="ACHGT5_000696"
     CDS             complement(<1..837)
                     /locus_tag="ACHGT5_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN7216707.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
     gene            complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="ACHGT5_000697"
     CDS             complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="ACHGT5_000697"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN7216708.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3338..3517)
                     /locus_tag="ACHGT5_000698"
     CDS             complement(3338..3517)
                     /locus_tag="ACHGT5_000698"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN7216709.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3564..5387)
                     /locus_tag="ACHGT5_000699"
     CDS             complement(3564..5387)
                     /locus_tag="ACHGT5_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN7216710.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5665..7944
                     /gene="helD"
                     /locus_tag="ACHGT5_000700"
     CDS             5665..7944
                     /gene="helD"
                     /locus_tag="ACHGT5_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN7216711.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8037..8684)
                     /locus_tag="ACHGT5_000701"
     CDS             complement(8037..8684)
                     /locus_tag="ACHGT5_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216712.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8863..9582)
                     /locus_tag="ACHGT5_000702"
     CDS             complement(8863..9582)
                     /locus_tag="ACHGT5_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7216713.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9713..10864)
                     /locus_tag="ACHGT5_000703"
     CDS             complement(9713..10864)
                     /locus_tag="ACHGT5_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN7216714.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11081..11821)
                     /locus_tag="ACHGT5_000704"
     CDS             complement(11081..11821)
                     /locus_tag="ACHGT5_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7216715.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="ACHGT5_000705"
     CDS             complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="ACHGT5_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN7216716.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12205..12798)
                     /gene="yqeK"
                     /locus_tag="ACHGT5_000706"
     CDS             complement(12205..12798)
                     /gene="yqeK"
                     /locus_tag="ACHGT5_000706"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN7216717.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12782..13432)
                     /locus_tag="ACHGT5_000707"
     CDS             complement(12782..13432)
                     /locus_tag="ACHGT5_000707"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN7216718.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="ACHGT5_000708"
     CDS             complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="ACHGT5_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN7216719.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="ACHGT5_000709"
     CDS             complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="ACHGT5_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN7216720.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14875..15402)
                     /locus_tag="ACHGT5_000710"
     CDS             complement(14875..15402)
                     /locus_tag="ACHGT5_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN7216721.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15582..16367)
                     /locus_tag="ACHGT5_000711"
     CDS             complement(15582..16367)
                     /locus_tag="ACHGT5_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN7216722.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16360..17229)
                     /gene="accD"
                     /locus_tag="ACHGT5_000712"
     CDS             complement(16360..17229)
                     /gene="accD"
                     /locus_tag="ACHGT5_000712"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN7216723.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17230..18609)
                     /locus_tag="ACHGT5_000713"
     CDS             complement(17230..18609)
                     /locus_tag="ACHGT5_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN7216724.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="ACHGT5_000714"
     CDS             complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="ACHGT5_000714"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN7216725.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19061..19537)
                     /gene="accB"
                     /locus_tag="ACHGT5_000715"
     CDS             complement(19061..19537)
                     /gene="accB"
                     /locus_tag="ACHGT5_000715"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN7216726.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="ACHGT5_000716"
     CDS             complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="ACHGT5_000716"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN7216727.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="ACHGT5_000717"
     CDS             complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="ACHGT5_000717"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN7216728.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="ACHGT5_000718"
     CDS             complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="ACHGT5_000718"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN7216729.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22567..22791)
                     /locus_tag="ACHGT5_000719"
     CDS             complement(22567..22791)
                     /locus_tag="ACHGT5_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN7216730.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22844..23806)
                     /locus_tag="ACHGT5_000720"
     CDS             complement(22844..23806)
                     /locus_tag="ACHGT5_000720"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN7216731.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23816..24256)
                     /locus_tag="ACHGT5_000721"
     CDS             complement(23816..24256)
                     /locus_tag="ACHGT5_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN7216732.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24562..25527
                     /locus_tag="ACHGT5_000722"
     CDS             24562..25527
                     /locus_tag="ACHGT5_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN7216733.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25584..25814)
                     /locus_tag="ACHGT5_000723"
     CDS             complement(25584..25814)
                     /locus_tag="ACHGT5_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN7216734.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25900..26787)
                     /locus_tag="ACHGT5_000724"
     CDS             complement(25900..26787)
                     /locus_tag="ACHGT5_000724"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN7216735.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26876..28099)
                     /locus_tag="ACHGT5_000725"
     CDS             complement(26876..28099)
                     /locus_tag="ACHGT5_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN7216736.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28116..29162)
                     /locus_tag="ACHGT5_000726"
     CDS             complement(28116..29162)
                     /locus_tag="ACHGT5_000726"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN7216737.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29326..31053)
                     /gene="ptsP"
                     /locus_tag="ACHGT5_000727"
     CDS             complement(29326..31053)
                     /gene="ptsP"
                     /locus_tag="ACHGT5_000727"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN7216738.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31053..31319)
                     /locus_tag="ACHGT5_000728"
     CDS             complement(31053..31319)
                     /locus_tag="ACHGT5_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN7216739.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31798..34032
                     /locus_tag="ACHGT5_000729"
     CDS             31798..34032
                     /locus_tag="ACHGT5_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7216740.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34180..34500
                     /locus_tag="ACHGT5_000730"
     CDS             34180..34500
                     /locus_tag="ACHGT5_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN7216741.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9787 a         7501 c         6050 g        11305 t
ORIGIN      
        1 cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa gcttttcgtt
       61 tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca aaagtgaatt
      121 agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga gtacatcggc
      181 tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga tattcatgct
      241 tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg aaacaagcca
      301 aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga tatctttttc
      361 aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta ctactaaaga
      421 agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact ctacgacttt
      481 ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt gtgcattgtt
      541 accaagtttg tttttgatag agtaaaagga ctcatttgct tctatatcac taaaaccttc
      601 atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca gactttgaaa
      661 accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag cttctgctaa
      721 ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt tggccaactt
      781 tttttctgct gaagaataat cttccatcat actttcaata cgactaatta cgctcataag
      841 ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc ttattcacgg
      901 tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt atcccatcaa
      961 tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct attgttcgga
     1021 ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct ctgctgtaat
     1081 ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca gctgtttctt
     1141 tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct ccaacaacta
     1201 attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt ttttcattac
     1261 gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt tcataagcat
     1321 aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt gccaaccatg
     1381 ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc attgtagaaa
     1441 caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct ccaacatatg
     1501 tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat gtttcttctg
     1561 taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct gcctcgcctt
     1621 ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt gcttgggctt
     1681 tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg cgatagctca
     1741 agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc aattcaagcg
     1801 tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca tcattcaacg
     1861 tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag cgatgcatca
     1921 ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg tttttgtaga
     1981 ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca tctcccatat
     2041 ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt ttgtacaact
     2101 caacatctcc cattattggt gtatatacat gttggtaacc tagactaact tctttgtcta
     2161 cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag aatggcaaac
     2221 cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt ttacgatggt
     2281 ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc ttgtcaaaaa
     2341 aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc caataagcac
     2401 ctgccactga taataattta aatacttgta tacggccagt ggatggaaca tgtggtccac
     2461 gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct tctggcaatt
     2521 cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct tcctgttttg
     2581 aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt tctgcttcga
     2641 ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg tagtaaaacc
     2701 ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga cgcatggctt
     2761 gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca tggtctggtg
     2821 tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg agttttccgt
     2881 tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca gcaattgcca
     2941 atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta atttttatca
     3001 taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa aaacaaaaaa
     3061 atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc acctaatgtt
     3121 caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct tcaacaaggg
     3181 tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag cttttcctct
     3241 cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt gtttatttaa
     3301 ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat tccccattta
     3361 caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct tttggtgcct
     3421 gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat tgttccggac
     3481 tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct ctttctgatc
     3541 gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga atcaggaaaa
     3601 atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc cacgacttct
     3661 tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa catcaaaaaa
     3721 atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt atctcttgaa
     3781 atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc atctgcactt
     3841 tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc agcagcaagt
     3901 gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc tttagggata
     3961 ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga atctaattgg
     4021 actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat catcaaattt
     4081 ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg aatctgatcc
     4141 gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc tgtattggac
     4201 gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc atgattattg
     4261 actactgctt ggattccttg accggtcact tctttcagtt gactaacagg atacagttgg
     4321 ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc ctgttctgca
     4381 ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg aatcgcctct
     4441 acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt ttttgtacgt
     4501 gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact tgaacggctc
     4561 attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga tgccactacc
     4621 agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc accgatcaaa
     4681 taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa tcgggcaggc
     4741 ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc tgcggcacgt
     4801 ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat aatctgatct
     4861 ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc gaccgaagat
     4921 tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac tagtaactgg
     4981 tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt gaatcgatgg
     5041 gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg tttattcgcg
     5101 taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact agcccagtat
     5161 tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac gccatatctt
     5221 cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat ccctccagta
     5281 aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact taataaagct
     5341 atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt tttttctcct
     5401 cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc atcttaagca
     5461 tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac tatcactatc
     5521 agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat tactttttcg
     5581 attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta attcgttatt
     5641 tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac gcacgtggat
     5701 gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca acaagaattg
     5761 actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg agctggttcc
     5821 aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga attgcttttg
     5881 aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt catgaaagat
     5941 agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac attatatcta
     6001 ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg gcgcgcacca
     6061 attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac tgaaacagat
     6121 agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg acgtatttta
     6181 tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat attggatgaa
     6241 tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca aaatgcaatc
     6301 attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag tggaaagaca
     6361 tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg gctcgaggct
     6421 gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc gactgtattg
     6481 ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt ggtgcagctg
     6541 ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt gctaggggaa
     6601 aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat tgatcgctat
     6661 atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa tggccgtacg
     6721 atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca attaccttta
     6781 catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg tctgcaaaaa
     6841 gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca agaactatac
     6901 gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg taaaaaactg
     6961 gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat gaattatcaa
     7021 tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact ttctcttcta
     7081 acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt tcgtacacac
     7141 ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact gatgcgccga
     7201 acatatccag tgacggttac acacaaagct cgctatatct ttattgatga aatgcaggat
     7261 ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa tttgactttc
     7321 tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc attggaagac
     7381 cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg ctccacaaaa
     7441 gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga aacaacagca
     7501 agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa attgaattgg
     7561 ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac agcaatcata
     7621 gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga gttgaaacaa
     7681 caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat tttacttcca
     7741 gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt agatgcagaa
     7801 catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg tgcgatgcat
     7861 gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat ggatatttca
     7921 tcttatgaga aaaaagtact gtaagtaaaa agacaactgc tatcattaat gttagcagtt
     7981 gtctttttat ttacaataat aattgcagaa aaagtaattc aatcatttaa ggtcatttat
     8041 tctatatata aatccaaatc tgtttcagga tattttaatt tgacaagcga aataatcatt
     8101 ttcagttctt ccatgttttc tggatattct gggtcaaata ccttgctatg acggccaatc
     8161 ccgccattgt cgatcaatcg gtaaattcga ttccattcaa tgaattggtc aattgattgg
     8221 aaattgctga ttggatagcc gccacaatcc aaaatctctg atacacgaaa tgctaggtat
     8281 gcactatcga tcactttatt ttttttcgtg tctatcttct catttctttc gataagaatt
     8341 ttttcaggac gatgtttcaa gctccactct aatagtagaa agaaagcctc catccgatac
     8401 tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg aatcagataa
     8461 ttttgtctat aaatcagcat tggtgcgcgg tccaccatac actctaaata acggttcaga
     8521 taccctgtac cgacatagtt tcctaatcga tagccttcaa cgccgaatct tgcgtatttt
     8581 tgcgctaatt ttgaacaatc agttacgttc acagctaact cttctacttt gcggtaaccg
     8641 cttaagatac gttcaaaagg ttctaaagtg atttttgctt ccatattctt ccccgtccat
     8701 cttccacata cttcatatca tttcacatga tacttatgct ctcatcatac cacaggttga
     8761 attagaaaag aaaataaatc acacaaaaaa gcaagacaat ctgttagtga gattgtcttg
     8821 cttttcgaaa aagactgaat acttttcgtt aaaactggtt gcttagtctt ctaatgccac
     8881 attatggaac acatgctgta catcttctag atcttccagt gcatcaatca ttttttcaaa
     8941 tgtttcttga tcttctggag atagtgttac ctcgttttga gggatcattt ggatctcagc
     9001 gattgaaaaa tcagtgattc ctttttcttt taatgcttct tgtatactat ggaaatccgc
     9061 tacttcaccg taaacgatga tttgtccttc ttcttccatt acatcgcgaa catcgatgtc
     9121 tttttccatt aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag caaagattgc
     9181 tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc catttttccc
     9241 aaatgctgcc cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg tatcaacaat
     9301 caccattgag ccgcttggtc caaatccttc atagcgcaat tcagagtatg tttcatctcc
     9361 tgcacctttt gctttttcga ttgctcggtc aataatatgt tttggtacat tataagtttt
     9421 agcgcgatca atgacgaaac gtagtttttg gtttgcatgc ggatcaggat cacctgattt
     9481 tgctgccgcg tagatttcaa tcccaaattt tgcataaatc cggctattat tagcatcttt
     9541 agcagttttc tttgctacga tatttgccca tttacgtccc atattttcac ttcgctttct
     9601 gatttgatag tttcataacg caaaaacgtc ctgttccatt atacttgtga atcacgattc
     9661 tgtgaagctt tttttctttc ttccttcatt cttctcattt ttagtcaatt atctatactc
     9721 ggattgggat ttgcccgaaa ttctgttccg gaatattgcc tcccaattga tagatttgat
     9781 cacttctaac taacagttcc gcttcttgtg cctctttttt tccgattcgg gaataaatag
     9841 aaatctcttt tttttctttt aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa
     9901 gataattttt attttgttgg acaggaatct cttgcttttt gatattcagc aagacataac
     9961 aagataatcg ttggatccgt gtccatgtat atctttttgt ttttaccttt gacacaaaat
    10021 cctcaaaact atcagcagtt gcagctgctg ttttcatgcg cgattctatt ccttcggtca
    10081 tctgatagat atctgtcagt tccagcaaag tagaagataa caaccgataa cgcaaatacg
    10141 gccaataaga ttcccaagtc tgcagcgaac aagttgaaag atcttcttct gtctgaggcg
    10201 gcacaagtcc agctattctt tttccttctt ctaacgcatg gcgaatggct gttgcactgg
    10261 ctatcctctg atctgtgatg attttttcat gatagcctgc acttttacgt cggatcggat
    10321 gtaattccat agggtggaca tacttggcat tttcctttgc ataacttaat cccaagatat
    10381 gatttggcga agaaaaatca agcgtaatat cgggatacag actgcgaaaa acctcagtca
    10441 ttttttgcgg ataactatac gtttgatcag acagttcttg aaatgcctct tctatcctat
    10501 cttgattctc ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg
    10561 ttccaaaaca caaactgtca cagtccagtg tttgcaagat ttttactgca cctttagcaa
    10621 aatagtcagc tgactgaacc gcccattcga cgggaagctc cactactaaa tccacaccat
    10681 tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat agccggttcg cctctttgca
    10741 aaaaattccc gctcattaca gcaatgacga cctctgctcc tgttattttt cgtgccattt
    10801 ccacatgata gcgatgtcca ttgtgaaaag gattgtactc tacgataatc ccgcagcttt
    10861 tcatgcgcat cctccttcat agttatcgat aaaaaagaaa tagagacaag taaaaaaagg
    10921 caaaaatcgg aaaaaatacc cgaactatat aaactattct cgcaaacaca atacttctgc
    10981 gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc ggatatataa tttatgttga
    11041 agatttattc ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg tatgctacaa
    11101 agaaccagcg ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga
    11161 ctttcgaaaa gccgctgttt tccagcattc ttaaataact ttccaatgga taggtacgct
    11221 cttgatgtag ttcatcttca cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa
    11281 aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag
    11341 caaaattatc cccttgataa tgatagctat attctggaaa aacctcatct acttgatagg
    11401 tggagtgtac gtcaaataaa aatcggccat tttcttctaa cgcttgatag acttcatcaa
    11461 acacttgctg tacctcttgt ctatttttca tatagcacag cgaatcagaa aaacaggtaa
    11521 ttgcctgata ttgtccgatt tctgataaat cgagcatatt gccttccaca aattggatcg
    11581 atgcgtcttc ttcatatgct cgctggctgg caatcatcaa catctcttct gaaaggtcta
    11641 accctgtcac tttatatcca tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg
    11701 ccagttccaa aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt
    11761 gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt tcataagcca
    11821 tatgttaatc caaccattca ctaagatcga ctagcggagc atctgaccat agtttttcca
    11881 gattatagaa atttcgttct ggcgcatgga aaacatgtac gatcacatct ccaagatcga
    11941 tcaataccca ttttccgcct tcttttcctt caatacgttt tacttcatat ttgttttctt
    12001 cttctttatc aatgatttct tctgtaattg cgttgatttg acgttcgcta tttgctgaac
    12061 agatcatgaa ataatctgcc aacaaggaaa catttcgaac gtctaaagcg ataatgtctt
    12121 ctgcccgttt tgaatctgct gcttcaatcg caatttttaa ttgttcttta ctatctatga
    12181 tgatttcctc ctaatatgag cagcttattt ttttgctacc cagtaattat atgtttcgat
    12241 tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc
    12301 aaatgctaca gcttcgtcta gatcagccca tgcaatggct cgtgcatctt gtaccccagg
    12361 aaaatctcga cctggttcaa tatagtctgc tacataaatg attttgtcaa ggagactcat
    12421 cttagcagcc ccagtcgtat gcacccggat tgcatgaaga atctctgcat cggtgattcc
    12481 taattcccgc tctacaaacg aagcgccaac aagaccatgc cagattgcat tattataatt
    12541 caataactcc ggatcaaaac cgtctcttac gattaccatt ttgaactcct catctggtcg
    12601 ttcttttgca taatcatgtg tcaatgcagc aatactagct ttttcaggag aagcaccgta
    12661 ttttttggca agagccacgg ctgtttcttc tactccaagc acatgcttga agcgttgttc
    12721 gctcattctc atctgtattt tttgcatcag gttttctcta gaataagcag tatattcatt
    12781 actatagtcc atattcatac aacccttcat tttgaatata atcaattact ttatctggaa
    12841 caaggtagcg gatagaacag ccttctttga tcttttgacg tattttcgtg gaactgatgt
    12901 cgatctcagg tacatctacc caaattacag gatatggagt atccgttgtg tatcctggtc
    12961 gacgtatacc cacaaagttg accatagaag tcagctcatc gattttatac cattttggca
    13021 aatattctac catatcccca ccgataataa agtaataatc tgtatccgga ttgttttgcg
    13081 tcaattcctt catagtatca taggtgtaac ttttcccacc acgagctaat tcaatcgttt
    13141 caatctgcaa aaaaggattg tcttccaccg ctaattccaa catgttgagt cgatgttttg
    13201 catcaatcgt ctgtttctca tcaacatgag gtggctgata agaaggcatc aaaaatacgc
    13261 ggtcaagacc taaattccgg ccagcttgtt cagccatcac taaatgtgca aggtgaacgg
    13321 gattgaatgt tccgcccaag atccctactt gctttttctt ctctaagaat tgaggcattt
    13381 cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg cgtattcatc atggtcactc
    13441 cttatattgc ttttacttct tttgatagcc tttgatactt ttcttttgtg gaaggtttgt
    13501 ataatacaat aacacgtcca atgatttgga cgatttcgca atctatttct ttttctaata
    13561 cttgagctgc ttcttctgca atctcatctg tattttgaag taacgatact ttgatcagtt
    13621 ctcttttttc taaagcttct tcaatttgaa cgatcattgc tgaattgatc ccaccttttc
    13681 cgatttgaaa aatcggctga agatgatgcg ctttgctacg taaaaaacgc ttttgttttc
    13741 ctcttaaact cataattttc cttcttcctt aaatcaatgc tttgcgtttc aatacatcta
    13801 caccctttgg cgcccagccc gcaattacta ctggcgctgt tacagtgatc cagcctagac
    13861 cggcaaaaac gatgtctgtt ttttctttca cagaaaactc aaaacgaaca agctccggaa
    13921 atgcctcgac ttcatcagga cgaggaggct gtaataatcc acctacatgt ttttgataaa
    13981 attcgtctgc tttttccagt ttcgttcgat gaatatttac atcatttgac acataagcaa
    14041 taaatgaagc acgattgccg ctgataaaat caaatcttgc aagtccgccc aagaaaagtg
    14101 tttgcccttc attcagctga taaactttag gtttgatttc ttttaacggt gcaatgatcc
    14161 gcaaatcttt tttacctaag tagtgcgcca tttgatggcg atggataatt cccggtgtat
    14221 caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt atccaaggtc gttcctggaa
    14281 actgcgaagt tgtgattaca tcttgtactc ccgctgtatt tttgatgatt tgattgataa
    14341 gcgtcgattt tccgacattt gttactccga cgatatagac atcccgtcca tcgcggtatt
    14401 cttcgatttt ttctaatagt tccgtcatct catgagattt cttagcacta gttaataata
    14461 catctactgg acgaattcct gcttcgtaag cacgttcctt catccattgt gttaatttag
    14521 tttttttcaa tgatttaggt aatatatcca ctttgttccc aactaaaagt actggattgt
    14581 caccgacaaa acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa atatccacca
    14641 cattgacgat caatgcatct ttttgcccta attcattcag taagcgcaaa aagtcgtcat
    14701 ccgtcaatga gacatcttgg gtctcattat aatggcgtaa tctgaaacaa cgctgacaat
    14761 agacttcccc ggaagctaaa cctttttcca aagccgtttg cggcgtgtat cctaattcac
    14821 cgggtttgtt tgtttgtata acggcaccac agccgataca ttgtaattct tctgtcattc
    14881 gattccacct cgccagccca tatcagggtg ttttgctaat agataggcca taattctttt
    14941 ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt gcgataatgg gttttacaag
    15001 gatgctacgt atccctgcac gattggctcc acgtatatca gtcataatct gatcgcctac
    15061 cattactatc tcatcatctg ctaaatccaa ttgtttcttt gctatgttga tccctcttgc
    15121 taaaggtttc attgctcgtg caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac
    15181 acgttttgaa ttattgtttg aaacaacaac aacagggatt cctgcatttt tcatttctaa
    15241 aatccaagta agcaactctt ctgttccatc tggattattc catgcaatca acgtattgtc
    15301 taaatcggtc aatactgctt tgatgcctag attctttaat tgggcaggtg taatctgata
    15361 gatggcttct accatccatg ttggtttgta atttgaaaac atgctgtctc ctcatctgca
    15421 aaaaatgaag gagcacataa agtactcctc aaccccgtat tatactgcat atcagcaaaa
    15481 aaaactacct ctatactcgt tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa
    15541 aataaagatt gatcacagaa aaatttagaa acaaaaaaca actaatattg gcggaacctc
    15601 ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa caagttccgt caatctttca
    15661 atcaacgctt ttttcataat gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac
    15721 gtttcaggaa taactcgatc aacgattcct aactcgcgta gttctggcgc agtaattttc
    15781 atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct
    15841 tctggagaaa gcacagcata aatcgtttct tccagcatcc agacttcatc cgctaaacct
    15901 agagcaaggg caccgccact tccaccttca ccaatgatga tggcgatgat cggtaccttt
    15961 aaatcagaca tttcgagcag atttctggca atagcctctc cttcgccgcg ctcttctgct
    16021 cctacaccgc aatatgcacc tgctgtattg atcaaggtaa tcaccggtct attgaacttt
    16081 tccgcttgtt tcatcagtcg taaagctttt cgatagcctt caggatgggg agaaccaaaa
    16141 tttctgcgga tattttcctg cagatcccgt cctttttgaa taccgatcac tgttaccggt
    16201 ttgccatcta atcgagcgat tccaccaaca atcgcttcgt catctgcaaa taaacgatca
    16261 ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt
    16321 tctttagctc ttgctaactg aacgacatca tgtgctgtct tatccacgat ctatccatcc
    16381 tttctgtgta tgcaattcaa gaagcgtatg gattttctgt ttcaattcca tgcggggaac
    16441 aatctgatcg acaaaaccat gagataataa gaattcagcc ttttggaaat cttctggaag
    16501 ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca aaaccgatca agctttgggg
    16561 ttctgctaga ataatatcgc cttccattgc aaaactggca gttactcctc cggtcgtcgg
    16621 gtccgttaaa acagttagat aaaaaagacc ttcattgcta tgccgtttga cagcagcaga
    16681 gatttttgcc atctgcatta gagaaaagat tccttcttgc atccgtgcgc cacctgaagc
    16741 tgtgaacaag acgactggaa gactttcctt tgttgctcgt tcgaataatc tggtgatctt
    16801 ttctccgaca atcgttccca tacttcccat gataaaatta ggatccatca ctccaataat
    16861 gaagggaatc ccctgaatgg ttgctttacc agtgaggact gcttcatgaa gtcctgtttt
    16921 atcttgcatt tttttaattt tttccgtata ttcgggaaat gctagcggat ctttggttac
    16981 aagatccgta tcccattctt caaaactttt ttcatcaata atcaaagcta gacgctgcca
    17041 tgcaccgatt cgaaaagcgt atccgcaatg agggcatact ttttcttcac cgatgtcttt
    17101 cgtataaagg atatgtttgc aatttggaca ctttgcccac atgttatcag gaacagatgg
    17161 cgagttagca ggtttgctct ctctattagg attgatccga atatatttct ttttcttaaa
    17221 cagagccatc tatgcgcctc ctatctccgt ttctggtgtc caattcggta aaaattcttc
    17281 ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca ggatgactaa tcaaatccat
    17341 ctgaaattct gcgttcgtga tgattccttc tgtgataaat tcacttaagg ctcgctgcat
    17401 cttcattaat gcttcaaaac gcgtatttcc atggacgatt atttttgcaa tcattgaatc
    17461 ataatatggc gggatcgtat atcccgggta aactgcactg tctactcgca gtcccatccc
    17521 acctgctgga agcagtaagt tttggatctt ccctggagaa ggagcaaaat ggaaagcagg
    17581 attttctgca ttaatccggc attcgattgc gtgtccttgg aatactactt cttcttgaca
    17641 cacattcaaa ggctcaccgg ctgctatctc cacctgtttt tttactaaat cgattcctgt
    17701 gaccatttct gttactggat gttccacttg gattcgagta ttcatttcca tgaaataaaa
    17761 atcgccttct tcatccatca gaaactcgat cgttccggca ttttcataat tgactgcttt
    17821 agctgctcga acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga tcactacaga
    17881 aggagattct tccagtactt tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa
    17941 atgaatgaca tgaccaaatc gatcacctaa gatctgaact tctatatggc gagcaggata
    18001 aatgattttt tccaaataca tatcatcatt tccaaatgcc gctgctgctt cttgctgagc
    18061 tgaagaaaaa tgctgtggga gctcttcttt tgattgaact ttccgaatgc ctttcccgcc
    18121 accacctgca gctgctttca gcatcactgg ataaccaatt ctgtctgcta tttccaatgc
    18181 ttcttctact gtagataata caccagtact tcctggaatg actgggacat ttgcttcttg
    18241 catcaattga cgcgcattga tcttatttcc cattgcatcg atcgttgcac ttttaggacc
    18301 aataaatgtg atattgcact cttcacacat ggaggcaaat cggctatttt cagataaaaa
    18361 accaaatcct ggatgaattg cttcggcttt tgtcacgatt gcagcgctca gtacttcctg
    18421 aacattcaga tatgaatcaa cggcttttgc cgggccaatg cagattgctt catctgctaa
    18481 ctgtgtatga agtgcttcgg catctgcttc tgaatatatc gctacggttt ggatccctag
    18541 ttcgcggcat gcacgaatga tacgtacagc gatttcaccg cggttagcta tcaatacttt
    18601 agaaaacata tttcacctat ccaatcataa aggttaattc cgcttcggct acttttttac
    18661 cgtctacttt agcgattcct ttgcctattc cggctgaagc tttcactttc aatatctcaa
    18721 cttcaaggta taaggtatct cccggtgtaa ctttcttacg gaattttgct ttgtccaatc
    18781 cgccgaaata agccgtcttt cccttgaatt gttcaagaga aagtaatgca actgctcctg
    18841 cttgagccat tgcttcaaca atcaataccc ctggcataac aggttcttct ggaaaatgtc
    18901 cttgaaaaaa gggctcattg atcgttacat ttttcttagc aacgatccgt tctccttcaa
    18961 ccatttcttc cactcgatca atcaaaagca ttggataacg atgtggaatg atttctttta
    19021 tttcttgtat gttcattgtt ttccctcttt cctgctttgc ttatttttgc tgaatcctaa
    19081 agagtggctg tccatattcg acaactgact cgttttctac aaggatttct gtgatcactc
    19141 cgtcggcctc actagttatc tcattcatca gtttcatcgc ttcaataatg cagataactt
    19201 ctcctttggc aacttgatca cctacttttt tgaaagctgg tttgtcgggc gctggttgca
    19261 gataaacgat tcccacgatt ggcgaaacaa cctcgattcc atcaaatact gccgattctt
    19321 tttctttcat tatttctgga gcagttgttg cttgttcagg tatgacattg actggctccg
    19381 aaactggcaa cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt ttatttttat
    19441 tcatatacaa ttcaaattga ccttcacgca aatcgaattc cgttagcgag gattgatcaa
    19501 attgactgac taattctctg atttcattga tatccaaaat tattcctccc agcgtttcat
    19561 gcacagaacc gcattatgtc cgccaaaccc taatgaatta ctcattgcat aagtaaaact
    19621 tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc tcaggatctt gtttttctac
    19681 attgatcgta ggtgggataa attgatgttc taatgccagc aaagttgcca aggcttcgat
    19741 cccgccggct gctcccaata aatgtccagt catcgatttc gtgctgctga cgtaagtatc
    19801 tctgaaattt tcacctaatg cgatttggat tgcttttgct tctgcctggt cattggctgg
    19861 tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc ggctgtacac cagcttcttc
    19921 catcgcaagc ttcattgctt tagatgcacc agaaccatct ggagtaggag ctgtcatatg
    19981 gtaagcgtca cagtttgcac cgtatccaac gatttctcca tagattttgg caccgcgagc
    20041 ttgtgcatgt tctaaagatt caagaactag aacgcctgct ccttcaccca tcacaaaacc
    20101 attccgattt tcgtcaaacg gaatcgaacc acgattagga tcagttgatt ctgagagtgc
    20161 tgttaaagct gcaaatccag caatccccat ctcacaaatc gttgcttctg ttcctccagc
    20221 aagaatgaca tcagaatagc catgtttgat gttacggaat gcttccccaa ttgaattcgt
    20281 tccgctagca caagctgtta cgatagctgt acaaatacct tttgcaccta cacgcaaagc
    20341 aatatttcca gctgccatgt tggcaatcgc cattggtacg aacattggag aaactcgttt
    20401 tgcacctttt tcatgtagac gaataacttg gttttcgatt gttggaagac caccgatccc
    20461 agatccgatg atgacgccaa accggtcttg attcattttt tctttatcta aaccgctcat
    20521 ctccattgct tctaaagcag cgtagattcc atagatcgaa aacatatcca ttcgtttgct
    20581 atctttttta ataaaatatt tatcgtaagg gaaatctttg acttcacccg ctaaggtaat
    20641 ccctgtttct gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc cctcttttaa
    20701 gctattcaaa aagctgtctg cgtcatttcc aataggtgaa gtgactccat agccagtaat
    20761 taccactcga ttcatttgtt tgctctcctt ttcttatccg tgcatgacca atccgccatc
    20821 gacattgatg acttgtccgg tgatatatgg attttgtgct aagaaaactg ctgtttgtgc
    20881 aatatcttct acttgtccga aacgctgcat cggaatttgc ttttccgctt gttccttgac
    20941 tttgtctgct aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa tggcattaca
    21001 tgtaatgcct ctagttgcag cttctcgtgc cacagatttt gttaatccta ctacgccagc
    21061 tttactagct gcataatttg cttgaccaat atttccaatc aacccagata cactagagag
    21121 attaatgatg gccccttctc gctgcttcat catttttttc aatacgtgct gcgtcatatt
    21181 gaaggttcca atcaaattga tatccagaca ttttttgaaa tcttctgcat ccattcgcat
    21241 cacaagtttg tcatttgtga ttccggcatt gtttactaac acatggatcg atccaagctt
    21301 ttcttctgct tctttgatca tttgtcccgc tttctcataa tcagagatgt caccggaaac
    21361 tcctacgcat tttacaccga atgcttcgat ttcttctatc ttttcttttg gaatctctcc
    21421 gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc
    21481 gattcctctt gtactacctg tgataaatac attttttcct tttacatcca taacgttcct
    21541 ccttatagta ttgctattgt ttcatcaaat gtctgcttat tctcaacacg cagaactggt
    21601 attgttttat cgattttttt cataaatccg cttaatactt ttccggggcc gacttcgatg
    21661 acttgttcga tgccgattgt ttttaactta tggatactct catagaaacg aacaggttgc
    21721 atgacttgct gttcaagtag tcccgcgatt gtttctttct tcattatttc cgttgttgtg
    21781 ttgcttatga taggaaaaga aggttcagag aattgaatct gacttaggtc cttcgccagt
    21841 ttttttgctg ccggttctaa aatagctgta tgaaaaggcc cgctgacatt tagagggatc
    21901 attcttttga accctttttc ttttaatagt aaaacagctt cgtctactgc tttttcttcg
    21961 ccaccaatca cgatctgctg aggtgtatta taatttgcag gagatacgat gccgtacttg
    22021 ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt
    22081 ttcccgcttc cagctggggc agcttctgtc atataagcgc ctctttttgc tactaacgca
    22141 actgcttcag tgaaggatag cgcattacta gctactaatg ccgaatattc tcctaaactc
    22201 agtcctaaag ctgcatctgg gatgattcca tgctccttca ataagcgata atacgcgatg
    22261 ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc attttctgta
    22321 aagcaaagtt cagccatatc gtagccgaga atctcacttg cttcatcaaa tgtctgcttt
    22381 acaacggctt cttcatataa atcttttccc ataccttgat attgtgcacc ttggccacta
    22441 aataagaatg ctgttttcat gtttttctcc aatatatcca gaatatatgt ttgattcttc
    22501 ctattcaaaa aggccgagac attcctattt caggaaatgt cccagccccc aacctaattt
    22561 taatgattat gcagtttgtt tttctacgta agtcactaaa tcttgaaccg ttttgattcc
    22621 gtcttctgtt tcgattttca cgtcaaattc atcttcaatt tcattgatga tttggaataa
    22681 atccaagctg tcagcttcta agtcttcttg gatgtcagta gtcaatttga tttcttcagt
    22741 ttcttttcct aattcttctg cgatgatttc ttgaattttt tcgaatacca taataatata
    22801 ttcctccgat attgtttgct ttttattttt ataatttaac agtctacaac tccatgagaa
    22861 tacttcccca tgttaaacct ccgccatagc ctgtaaatat tactttctgc tgttttccta
    22921 aggtgatctt tccactagaa actgcttcat ccaataagat agggatgctt gcagcagatg
    22981 tattcccata tttatccata ttggtcagga acttttcttg cggcatcttt acttttcggg
    23041 caattttctc aataattctg aggtttgcct gatgcaaaag cagataatca actggtttgt
    23101 ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc
    23161 tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac ggatttttat
    23221 tttgctggta gccagcggtc aatgagctac ttctggtacc gtcagctgct aatttttctt
    23281 tcagcaattt tttctgtgga ttcgcttcta agagaatccc acctgctcca tcgccaaaca
    23341 acacagcagt cgtacgatca gaccaatcga ttagcttaga tagtgtttca ccaccaatca
    23401 ccagtccctt agttgagccg cagcggatca ttttttcagc catgcttaac gcataaacaa
    23461 atcctgagca cgcagcactg atatcaaaag cgatagcatg agttgctccc aatttccctt
    23521 gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga cattgttgct actaaaataa
    23581 aatccaaatc tttagggttt agatttttct gttttatcaa tttttccgcc actaaaatgc
    23641 ataaatcaga agtctcctga tttgttacac atcttcgttc acgaataccc gtccttgacg
    23701 aaatccattc atcagaggta tccatcatct tggaaagatc attattagta ataatctttt
    23761 caggaaggac gctggctgtt gcagtaatca ttccaaattg ttccattaaa tatcctcact
    23821 tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct
    23881 ccgtagccat tccgtctaag atgttcttga ccatttcacg atgaaaatgc tgatggaccc
    23941 gaaatagtaa ctttcctttt ttcgttagtc ccaactttac aacgcggcgg tcatcttcac
    24001 tgcggatacg ttcgacatag ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc
    24061 caacagtaat tgatagttct ttggctactt ctgaagttgt ttttttcttg tacatcccga
    24121 ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt gaattgagat tttttcagct
    24181 cagactcttc gattgtcaaa atatcattga agacactaac aagataatca tttactgttt
    24241 ttaaatctgg ttccatgttc caccgtccac taattagttt gattatcaaa tcttttgatg
    24301 ttcaaactat atcttaactt ttttcaaaat gcaaccttaa aagatcattt ttttactata
    24361 tgagcttttt ttaagatttt actcatttaa aacaactgat aaatcagcat ttataatttc
    24421 ttttattaga aaaatcatct atctcacata ttttgattat caaagtattt tctgtgaaca
    24481 gaactataga attccctaaa acttgtccat tatttcaaaa tttgttatag tctttttatt
    24541 caaagcgtaa aaggagatcc tatgttaaaa gaaaaaatga aaactctccc catgctgccg
    24601 ggtgtctatc ttatgaaaga ccgagatggt aaggtcatct atgttggtaa agcaaaacgg
    24661 ctcaaaaata gagtaagcag ctattttcat cagaataaac agcattcaaa aaaagtatta
    24721 cgtatgattc atcatataac agattttgat ttcgtcgtag tagacacgga attagatgct
    24781 ttattgctcg agtgtcaatt gatccaacat taccgtcctt tttataaccg acaaatgaat
    24841 tatttcagta attataatta tgtacatatt accaataagg gatttgtatt aacagatact
    24901 cctacggctc gaacatacgg gccttttcgt ctctataaaa aaatgccgtc tattttgcgt
    24961 ataatggaag aaacttatca aatgccttgg ttgtctgaaa ttagtttgtt agctttgcga
    25021 gtccagcttc ctgacctaca ggaaatgact tttgaacaga aaaaaaagga actccaaggg
    25081 ctgtttcaag gccgaaataa aaagctactg acttacttga aaaaaagaca acaacatttc
    25141 atctatcagt tgaatttcga aaaagccggt atgcttcaaa aagatatcga attagttact
    25201 tattttatta gacggatcca ggaacaaaag cagtttttaa gaacaccatc gctcaccttc
    25261 agcatgccat tagcagcaga tgagagtcag aaaaagcact acctcgtttg ttatggtcaa
    25321 cttgcagaaa caatgattac ctctggtgac gtttctcctg acttctatta tgaaaagaaa
    25381 gaagctcatt tatctttgaa acgtcaattg tcaaaagaag aaatcgatcc tgtacaaatc
    25441 ttgatcagtt accgcaaaaa attagaaaaa gaacagtttg aaattgaact gttaaacaaa
    25501 aaagaagctg aaaagcaact aaattagttg cttctcggct tcttttaggc atcttcaact
    25561 tcaaatgtca attatcctaa aatttattgg attaagtcat agatttccat cgctacgata
    25621 tcgatattat caaattcgta ttgctgatga gtatctgttt cagttaattc aaatgtatcg
    25681 gtcgctttat cataagttac tgtacacttc tctacgcctt ctttttcaaa acggcgaact
    25741 tgcagttcat tatctgctga ctcgaccata gcctccagtc gttttatgat tgcgactaat
    25801 tgtgaatgtg tcatcggagg tcctcctttt cttatttctg ttatattgta tcaaaagcat
    25861 agcctacttg cattgatttt ctctaaaaaa actcgttttt tatgattttt tttctggtat
    25921 ttttccttca ggccaccaaa gtttgatcaa agcttgggtt ccgtcattct caaaaccttt
    25981 atctgccaac tcttcatata gtttcaatgc ttgggtagtt gctggcaata caagatccat
    26041 tttttctgct tcttctaatg caatctttaa atctttgata aaatgtttga caaaaaaacc
    26101 aggtgtgtaa tcttctttta aaatacgagg agaataattg cttaaagacc agttagctgc
    26161 acttccacct gaaagcgtct caatcacttt ctttaaatca agtccgtttt tttgtgcata
    26221 gacaagcatt tccgtcattc ctgtcatcgt acctgcaatc atgatctgat tagccatttt
    26281 tgtatgctga cctttcccag cagaaccatg caatgtaaat gtcgtaccaa attctttgaa
    26341 taaaggcagt acttgatcat aaacggcttc ttctccaccg accatgatcg tcagtgtacc
    26401 attctttgct cctaagtcac caccggatac cggtgcatct aacgcatctg cgcctttttc
    26461 ttttgctgtt tttgcaattt tttctgccag gcttggtgta cttgtagtca aatcaactaa
    26521 aatatgtcct gagatatctg cagaaaaaat ccccgactgt ccatagtaga tttcttctac
    26581 atctttgggg taacctacca ttgtaaagag aatattactt tgttctgcta ccgcttttgg
    26641 tgtatctgcc cagatagctc cttctgttac taaaggatca gcttttgctt ttgtccgatt
    26701 atagacagct acttcgtgtc ctgcttctag tagatggcgt gcgacagcac ttcccatgac
    26761 gcctgttccg ataaaaccaa gtttcatttt ttcgacctcc taaaaatcaa aaatccttgc
    26821 ttctatgcta gtatacgcta gaaacaagga ttttttcata aaaaatgttt gaattttatc
    26881 ttttaaatga atttaatttt attttgattc gttcaatcga gtcttcttct tcttttcttc
    26941 gcatctcttc ttcatgataa acaaccatgt cttgataaaa ttcgatcata gcttcgccga
    27001 aatgtgtaga tgaaatatca taaagtttag cttctaaaac ttttggatct ttaggaactt
    27061 cactggaaat ataatcaata agtgctggtg caaagtcttc gtcggtttca aaagttttcc
    27121 ctaatgaagg atgatcgaac aatcggttca aataagcatt tccttccacg acacaaggtg
    27181 ttccggctgc catcgcttct gtataagtaa gcccttgtgt ttctgaagta gaagcactga
    27241 caaaataatc tgccgcttta taataaagtg ctacatcatt gttgtccact tctcctgtga
    27301 actgcacaaa ttcttctaac tgcagttctg agatcaattc ctctaactct tctacataag
    27361 gtccttttcc tacgataact aatctggcgt taggataagc ggagatgacc tgaggaaacc
    27421 cattaatcaa ggcttggata tttttctcat aagaaatcct gcttaaagaa agaatcatca
    27481 aatggttttc ctgaagacct agtgactccc ttaatttgct gatatcctgc tgagtgatat
    27541 cagaacgttt gaatttttca atgtcgatac cagtaggaat cacgcgaagc ggtgctatta
    27601 caccatagct gcgtaatgta tcaataacac gttcacttgg acaaactaca cctgtcgtat
    27661 gatttgcaaa taatctagac aagtatttta catgggattg cctgatcact tttcctttac
    27721 cgatataatg aaggtagtct tcatacatcg tatggtaagt atggatcaga ggtattttca
    27781 acttcttagc taccatcttc ccaagtatcc ctgctccgaa ttctgtatgg gtatgaataa
    27841 gatctagttc tagttctttg gcaaccaaat aagcatacca catgcctcta acaacaactc
    27901 ttcgatcctt gaaagaaata aatggtacac ttggcattcg aatcacatct ttttccaact
    27961 tatccgcatt aggatcggtc gttgtaaaaa tatagacttc atgcccatgt ctttctaatt
    28021 cttgttttaa tgttttgatc gatgtagcaa ctccgctgac ttgcggaaag taggtatcgg
    28081 taaaaaagcc gattttcatg tttttcgcct ccaaactagt ccttgacctg atttactttt
    28141 tttaatttgc tagagacttt ttcattcagt acactttcgt atacattttt aagttcttca
    28201 ccgattttat tgatacttcg ctcttttgct gtttgataac cggcttgtgt cgtactcggt
    28261 agttcttgat tggtaatctg ttggatcagt cggataaatt cttcgtttgt cttccccata
    28321 tagcagttct tgctatcttc taaccagcca ttatacactg gaatatctcg aacaagtacg
    28381 ttttgtttgc ttgctaaagc ttctaaaaca acgatccctt ctgtctcttc ataagaaggg
    28441 aaaaagaata gatctgcacc tgaatacgca ccttctatga tctctccacg aatgtaacca
    28501 ggaaacagga cattttcagg atgatcttct tttacaattt tccggatgtt ccgaggaatc
    28561 gagtacatgg gaacatgacc aaaccaaata aatgtgtatt cgggcatctt cttagcaatt
    28621 tctacgaaat caatgattcc tttacgttcg aaaaacaaac cgacacaaat gattactttt
    28681 tgatccggag acagtttgaa atattctctg aacttttgtt ctttcattgg atcagggtga
    28741 tatttctcta agtcgatccc attggaaata gcttgaatcg gcacacggat accatagctt
    28801 tccaacaact gttttgaata aggtgttggc gtgatcagat agtccgcttt ttggtacaat
    28861 cctaccagat atttttttac gataggagaa agctggttcg atctgataaa ggaatttcgg
    28921 aaatcttcct ctgtagaatg cgcatgatag acaacttttt ttccttctct tctggcttta
    28981 ttgaccatat tatgactatt gattccgtat gtattgatgt gcaggatgtc ataatcttct
    29041 ttttcatcta gtgtataaga gataccgact tccgtcagag cacgtttttg atgatcaagc
    29101 gcacgcccga ttccagattt tgcaagcatc ttttcacttt caaaatataa taataccttc
    29161 accttacgtc ccccttcact tatatagtat aacatagcct acaaactgtc actcaaactt
    29221 aagccctatt tttgtctatt cttactgaca aaaacgtttt cttaaaaaag aacaaaaaaa
    29281 ctggcgtagc tcttttacaa gccacaccag cttttgatac gttgtttatt ttacatattc
    29341 atgaacaagt tcaacgactt cttccatcat atcgcattct ttcaatgcac gatcagcaag
    29401 ttcagccatt ttcgctgtgt ccaagcgttt cattaaacta cgcgttttaa gaatagatgt
    29461 cgcactcatt gagaactcat ctagacccat acctactagc aatggaacag ctgtttgatc
    29521 tccagccatt tctccacaca tacctgccca ttttccttca gcgtgtgctg catcaattac
    29581 atttttgatc aaacgtaaaa ttgatgggtt atatggttgg tacaagtaag aaacgcgttc
    29641 gttcatacgg tctgctgcca ttgtgtattg aatcaaatcg ttcgtaccta cagagaagaa
    29701 atctacttct tttgcaaact tgtctgctaa gactgctgct gctggaatct cgatcatgat
    29761 gccgacttgg atcgtatcag atacttcctt gccttcactt atcaattttt gtttttcttc
    29821 ttcaaagatc gcttttgctg cgcggaattc tttgagtgta gctaccatag ggaacatgat
    29881 acgtaaatta ccatgaacag atgcacgaag caatgcacgc atttgtgtac ggaacatttc
    29941 gtcgccttgt tcagataaac tgatacgcaa tgcacgatat cctaagaatg gattcatttc
    30001 atgaggtaat tgcagatatg gtagttcttt atctccaccg atgtccattg tacgaacaac
    30061 tacaggtttt ccttccatac cttctaatac agctttgtaa gcttcatatt ggtcatcttc
    30121 tgttgggaag tctggagaat ccatatacag gaattcagta cggtacagac caactgcttc
    30181 tccaccatta ttatggacac caactagatc ttttggtgtt ccgatgtttg cagctaattc
    30241 aaaatgcttg ccgtcagctg tgacagtttc tgcatttttt aatttttccc attctgcttt
    30301 ttgtgctgcg taatctgcac ctgctttttc aaattcagct ttttgttcat ctgtaggatc
    30361 gatgattaca tcgccttcga tcccgttgac agccaaaata tcgccttctt tgactttagc
    30421 agtaatttct tttgtcccaa cgattgctgg aatttctaaa gaacgtgcca tgatagcaga
    30481 atgtgaagta cgtccaccaa tgtctgttac aaacgctttt acaaaattgc gatctaattg
    30541 tgctgtatca cttggagtca agtcatgtgc aacaacgacc acttcttcgt tgatcattga
    30601 tggatttggt aaagtaacgc ctaataagtg cgctaaaata cgtttagcaa cgtcacgaat
    30661 atctgcagca cgctcttgca tataagcatt atcttccatt gcttcgaaca taccgatata
    30721 catatccgtt acttctttta gcgctgattc agcatttacg ctgttgtctt taatattttg
    30781 cttgatttga cctaccattt ctggatctga aagaaccatc aaatgggcat caaatacttg
    30841 cgcttctgct tcacctaagc tttgcgctgc tttttcacga atttgctgca attcttcagt
    30901 agatttagct aatgcagcat ccaaacgagc ttcttcagcc gcagtatctt ctactgttgc
    30961 cttactgaat gttaaatccg gttgaactag caggtaagct tttgcaacgg ctacaccgtc
    31021 actagcggca atccctttta gcatttcaac cattattcag ataagccttc tttcttcatt
    31081 gtttcaacga ttgctgccat tgcatcagct tcgtcagcac cttctgcagt aatagtaaca
    31141 tcagaacctt gacctacacc taaagacata acgcccatga ttgatttcaa gtttactgat
    31201 ttacctttat actctaagtt tacatcagag ttaaatttgc ttgctgtttg tactaatagt
    31261 gtagctggac gagcgtggat cccagtttct gctactacgt gaaattcttt cttttccata
    31321 ttaatcggtc tcctttaagt gagtgttgtt tttatgtgag ggttttcttt tttagtaatt
    31381 cagagaatcc taacaaatgc caccctttca tctgtaaaga ttaacatttt ttttcgttag
    31441 atacaacagt tttcattcaa tttcagataa aagtattcaa attattccgg attctttttc
    31501 atccttttct tcacatttta taacaaacgt gtttatttcc tttttcttgt cgctttctct
    31561 tctcctgacg tttatcattc tttcctttga ccctctttgt tttcatatga aacgaattac
    31621 ttgcttccct ttttattttt agtataataa gagtcaaagg tcaggaatgg tcaatagaga
    31681 tttcaccttt tctacctcat aaatctctta aaaaactagg ggttattctt ttacctcatg
    31741 aaaaacgaga tgtaaaatat ggatgtaccc atttttgtga aaggacgtga atgattcatg
    31801 ctttgtcaaa actgcggcaa gaatgaggca acgattcact tgtatgccac tgtcaatggt
    31861 cagcggacac aactcgatta ctgtcagagc tgttatcaaa aaatcaaaaa tcaacaaaat
    31921 ggaggtctga ctaatatggc ccaaaatgat ccatttggat ttggcagttt ggacgatctt
    31981 ttccgttcca tgtctcgcca aatgcaacag caaggaaatt atgagcaaac accaccaaca
    32041 caatttggcg gaaacaacaa cttcaatggt ggtcagccac ctcaaggcaa tacagaaggt
    32101 ttattgggag aatacggaat caatatcaca gaacaagcac gacaaggtga tatcgatcct
    32161 gtgatcggac gcgatgacga aatcaaacga gtcattgaaa ttttaaatcg ccgaaccaag
    32221 aacaatcctg tcttgattgg agaaccaggt gttggtaaaa cagcagttgt tgaaggttta
    32281 gcacaaaaaa tcgtagatgg tgatgtaccg caaaaattaa tggacaaaga agtcattcgt
    32341 ttagatgtcg tatctttagt ccaaggtact ggtatacgag gacaatttga agaacgtatg
    32401 caaaaattga ttgaagagat ccgtcaagca gaaaatgtga tcctattcat agatgaagtt
    32461 cacgaaatcg tcggtgctgg ttctgctggt gatggaaata tggatgctgg taatatttta
    32521 aaaccagctt tagctcgagg agaattgcaa atggttggtg ctactacctt aaatgagtac
    32581 cgcatcatcg aaaaagacgc tgctttagaa cgtcgtatgc aacctgtacg ggtagatgaa
    32641 ccaactgtcg aagaaacgat cagtattttg aaaggtttgc aaaaacgtta tgaagactat
    32701 catcatgtaa aatatactga tgaggccatc gaagcagccg caactttatc caatcgttac
    32761 gtccaagatc gcttcttgcc ggataaagcc atcgatttgc tagatgaaac aggttctaag
    32821 aaaaacttga cgatccaaat cgttgatcct aaaacaatcg agaaaaaact gcaagaagca
    32881 gaagaacaaa aagttttagc ttctcgagaa gaagatttcg aaaaagcagc ttattatcgc
    32941 gatcagatca ataaacttca aaaaatgaaa gaacgtcagc tcactgaaga ggaaacacct
    33001 gtgattaccg aaaaagatat ggaaaaaatc gttgaacaac ggacaggtat tccagtgggt
    33061 gaattaaaag aaaaagaaca gacacaactg aaaaacttag cagatgactt gaaagctcat
    33121 gtcatcggac aagataacgc tgtagatcga gtagcgaaag cgattcgccg aaaccgtgtt
    33181 ggattaaaca aacagaatcg tccaatcggc tccttcttat ttgttggacc aactggtgta
    33241 ggtaaaacag agcttgcgaa acaacttgcc tacgaactat tcggttcaca agattcaatg
    33301 attcgtttcg atatgtctga atatatggaa aaacatagtg tctctaaatt gattggttct
    33361 cctccaggtt atgtcggata cgaagaagct ggtcaattaa cagaaaaagt acggagaaat
    33421 ccttacagtc ttgtcttgtt agatgaagta gaaaaagctc atccagatgt tttgcatatg
    33481 ttcttgcaaa tcttagatga tggtcgattg acagatgcac aaggaagaac ggtcagcttt
    33541 aaagatacga tcatcattat gacaagtaat gccggaactg gtaaagtaga agcaaacgtt
    33601 ggttttggtg cggcacgcga aggtgttact cgttctgtat tgaaccaatt aaacaactac
    33661 tttacaccag aatttctgaa ccgttttgac gggatcatcg aattcagtgc attatcaaaa
    33721 gaaaacttga tgacaatcgt cacactaatg ctagacgatg tcaaccaaat gctggcagca
    33781 cagcaacttc acatcgaagt tcctacgaat gtcaaagaaa aattagttga cttaggctat
    33841 gatccatcta tgggtgctcg tcctcttcga cgcacgatcc aagaacaaat cgaagatggt
    33901 atcgctgaat tttatcttga tcacccttct atccatgagt tgaaagcaaa actggataaa
    33961 gatggaaaaa tcatcgttac ttctaaacca gaacgtttgg ctaaagaatc agccgaagaa
    34021 acggctgaat aaatccagtt ttatagcgtg agacggtatt tctttgagaa atgccgtctc
    34081 attctttttt cagaaaagtc aaaatactgt tcattttata aaaaagaaat tataataaga
    34141 gaaacaagca attaaaaata cataaaagag gtgagcgcca tgaaattggt aaatgtaaca
    34201 aacagccatt cccgtcttgt attaaaccag cttgaaaaca ctgatgccca tctagtcaag
    34261 gtctacacag ccggaaatac cactattgtc tacactgaag cccctgaaca taatgaaatt
    34321 ctgctgatca atgacaaacg taaaatccag ccgaaagaaa tcgaagatgc taaaaattat
    34381 ttcttagata aaatcaaaga cgccgtttac cacgaggatg aaatcaaagt aatcgaattg
    34441 gcaggccttg ttgaaatctc gattccaaaa catgaattag gcgctacatt agccgtgtaa
    34501 acaaattttg aacataataa ttaaagagcg tgggacaaaa gtaaagaaca cttttgtctc
    34561 acgctctaaa cacgtataaa cggcgaaagc agaagcaact ccttcggaaa taagctgaaa
    34621 ttcacgaaaa ttgcttttca aat
//
LOCUS       DAZBWR010000016        33781 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000016 DAZBWR010000000
VERSION     DAZBWR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.016"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACHGT5_000731"
     CDS             complement(27..1259)
                     /locus_tag="ACHGT5_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN7216742.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACHGT5_000732"
     CDS             1514..2083
                     /locus_tag="ACHGT5_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7216743.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACHGT5_000733"
     CDS             complement(2261..2701)
                     /locus_tag="ACHGT5_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN7216744.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACHGT5_000734"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACHGT5_000734"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN7216745.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACHGT5_000735"
     CDS             3655..4578
                     /locus_tag="ACHGT5_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN7216746.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACHGT5_000736"
     CDS             complement(4803..5042)
                     /locus_tag="ACHGT5_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216747.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACHGT5_000737"
     CDS             complement(5047..5466)
                     /locus_tag="ACHGT5_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN7216748.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHGT5_000738"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHGT5_000738"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN7216749.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACHGT5_000739"
     CDS             complement(7038..7673)
                     /locus_tag="ACHGT5_000739"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7216750.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACHGT5_000740"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACHGT5_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACHGT5_000741"
     CDS             complement(8015..8650)
                     /locus_tag="ACHGT5_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7216751.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACHGT5_000742"
     CDS             9162..10304
                     /locus_tag="ACHGT5_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN7216752.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACHGT5_000743"
     CDS             10297..10503
                     /locus_tag="ACHGT5_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN7216753.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACHGT5_000744"
     CDS             10582..11775
                     /locus_tag="ACHGT5_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7216754.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACHGT5_000745"
     CDS             11980..13119
                     /locus_tag="ACHGT5_000745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN7216755.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACHGT5_000746"
     CDS             13112..13912
                     /locus_tag="ACHGT5_000746"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN7216756.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACHGT5_000747"
     CDS             13912..14739
                     /locus_tag="ACHGT5_000747"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN7216757.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACHGT5_000748"
     CDS             14744..15451
                     /locus_tag="ACHGT5_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN7216758.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHGT5_000749"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHGT5_000749"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN7216759.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHGT5_000750"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHGT5_000750"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN7216760.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHGT5_000751"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHGT5_000751"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN7216761.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHGT5_000752"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHGT5_000752"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN7216762.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACHGT5_000753"
     CDS             19036..21069
                     /locus_tag="ACHGT5_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216763.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACHGT5_000754"
     CDS             21113..22393
                     /locus_tag="ACHGT5_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN7216764.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACHGT5_000755"
     CDS             22603..23409
                     /locus_tag="ACHGT5_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216765.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACHGT5_000756"
     CDS             23421..24641
                     /locus_tag="ACHGT5_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216766.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACHGT5_000757"
     CDS             24631..26217
                     /locus_tag="ACHGT5_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7216767.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACHGT5_000758"
     CDS             26256..28394
                     /locus_tag="ACHGT5_000758"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN7216768.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="ACHGT5_000759"
     CDS             complement(28762..29745)
                     /locus_tag="ACHGT5_000759"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN7216769.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="ACHGT5_000760"
     CDS             30123..31514
                     /locus_tag="ACHGT5_000760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN7216770.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="ACHGT5_000761"
     CDS             31530..32570
                     /locus_tag="ACHGT5_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN7216771.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..33674
                     /locus_tag="ACHGT5_000762"
     CDS             32589..33674
                     /locus_tag="ACHGT5_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN7216772.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT        10932 a         5807 c         6669 g        10373 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg
    33181 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
    33241 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
    33301 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
    33361 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
    33421 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
    33481 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
    33541 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
    33601 cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag agtatatata
    33661 gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta
    33721 tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga
    33781 a
//
LOCUS       DAZBWR010000017        33232 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000017 DAZBWR010000000
VERSION     DAZBWR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.017"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(282..623)
                     /locus_tag="ACHGT5_000763"
     CDS             complement(282..623)
                     /locus_tag="ACHGT5_000763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216773.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(720..1172)
                     /locus_tag="ACHGT5_000764"
     CDS             complement(720..1172)
                     /locus_tag="ACHGT5_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216774.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(1179..1727)
                     /locus_tag="ACHGT5_000765"
     CDS             complement(1179..1727)
                     /locus_tag="ACHGT5_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFN7216775.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1772..2104)
                     /locus_tag="ACHGT5_000766"
     CDS             complement(1772..2104)
                     /locus_tag="ACHGT5_000766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216776.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(2118..2375)
                     /locus_tag="ACHGT5_000767"
     CDS             complement(2118..2375)
                     /locus_tag="ACHGT5_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216777.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2570..3112)
                     /locus_tag="ACHGT5_000768"
     CDS             complement(2570..3112)
                     /locus_tag="ACHGT5_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFN7216778.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(3133..4224)
                     /locus_tag="ACHGT5_000769"
     CDS             complement(3133..4224)
                     /locus_tag="ACHGT5_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN7216779.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(4232..6442)
                     /locus_tag="ACHGT5_000770"
     CDS             complement(4232..6442)
                     /locus_tag="ACHGT5_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN7216780.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(6458..8986)
                     /locus_tag="ACHGT5_000771"
     CDS             complement(6458..8986)
                     /locus_tag="ACHGT5_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7216781.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(9038..9448)
                     /locus_tag="ACHGT5_000772"
     CDS             complement(9038..9448)
                     /locus_tag="ACHGT5_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN7216782.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(9461..9679)
                     /locus_tag="ACHGT5_000773"
     CDS             complement(9461..9679)
                     /locus_tag="ACHGT5_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFN7216783.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(9676..10668)
                     /locus_tag="ACHGT5_000774"
     CDS             complement(9676..10668)
                     /locus_tag="ACHGT5_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN7216784.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(10747..11658)
                     /locus_tag="ACHGT5_000775"
     CDS             complement(10747..11658)
                     /locus_tag="ACHGT5_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN7216785.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(11677..12021)
                     /locus_tag="ACHGT5_000776"
     CDS             complement(11677..12021)
                     /locus_tag="ACHGT5_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216786.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(12041..12319)
                     /locus_tag="ACHGT5_000777"
     CDS             complement(12041..12319)
                     /locus_tag="ACHGT5_000777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216787.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(12678..13178)
                     /locus_tag="ACHGT5_000778"
     CDS             complement(12678..13178)
                     /locus_tag="ACHGT5_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216788.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(13179..13724)
                     /locus_tag="ACHGT5_000779"
     CDS             complement(13179..13724)
                     /locus_tag="ACHGT5_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216789.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(13721..14977)
                     /locus_tag="ACHGT5_000780"
     CDS             complement(13721..14977)
                     /locus_tag="ACHGT5_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN7216790.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(15338..15649)
                     /locus_tag="ACHGT5_000781"
     CDS             complement(15338..15649)
                     /locus_tag="ACHGT5_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216791.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(15672..17147)
                     /locus_tag="ACHGT5_000782"
     CDS             complement(15672..17147)
                     /locus_tag="ACHGT5_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFN7216792.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(17596..18048)
                     /locus_tag="ACHGT5_000783"
     CDS             complement(17596..18048)
                     /locus_tag="ACHGT5_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216793.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(18050..18415)
                     /locus_tag="ACHGT5_000784"
     CDS             complement(18050..18415)
                     /locus_tag="ACHGT5_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216794.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(18514..22797)
                     /locus_tag="ACHGT5_000785"
     CDS             complement(18514..22797)
                     /locus_tag="ACHGT5_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFN7216795.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22944..23183)
                     /locus_tag="ACHGT5_000786"
     CDS             complement(22944..23183)
                     /locus_tag="ACHGT5_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216796.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(23273..23515)
                     /locus_tag="ACHGT5_000787"
                     /pseudo
     CDS             complement(23273..23515)
                     /locus_tag="ACHGT5_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23628..23864)
                     /locus_tag="ACHGT5_000788"
     CDS             complement(23628..23864)
                     /locus_tag="ACHGT5_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216797.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(24875..25785)
                     /locus_tag="ACHGT5_000789"
                     /pseudo
     CDS             complement(24875..25785)
                     /locus_tag="ACHGT5_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25981..27816)
                     /gene="lepA"
                     /locus_tag="ACHGT5_000790"
     CDS             complement(25981..27816)
                     /gene="lepA"
                     /locus_tag="ACHGT5_000790"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN7216798.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            27963..29183
                     /locus_tag="ACHGT5_000791"
     CDS             27963..29183
                     /locus_tag="ACHGT5_000791"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216799.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            29203..29697
                     /locus_tag="ACHGT5_000792"
     CDS             29203..29697
                     /locus_tag="ACHGT5_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN7216800.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            29675..30361
                     /locus_tag="ACHGT5_000793"
     CDS             29675..30361
                     /locus_tag="ACHGT5_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216801.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(30512..33121)
                     /gene="clpB"
                     /locus_tag="ACHGT5_000794"
     CDS             complement(30512..33121)
                     /gene="clpB"
                     /locus_tag="ACHGT5_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN7216802.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         9750 a         7019 c         5096 g        11367 t
ORIGIN      
        1 ctagaaaaat caaaataaga ttcgtatttt gattctatat atcctgtttc aactacatct
       61 tatatttcac aatagaataa atagcctttt gcatagctgc ttaacttata agaaattgca
      121 atttacttct tcaaagtgaa aacgaatatg agtaaaataa aattattctt cttacagata
      181 attaaaaata gcgcgtaaca aaaatcggca ttcaatcaca taattaggat tgatgccgat
      241 tacttatatt ctacgtatag ttttagtatt cttatttttt atcaccagta accaattgag
      301 aacagcccta aagcagaata gtcttttatc tcaaaacgct taatttcagg cttaggaata
      361 tgaataatct tttgagtata ttctttctct tttaatctta ctgaagaaat accactgcct
      421 atctttttta caaatatttc atttggtgta cagactaata aaacaatatt tgtaatcaat
      481 ccttgcacta aagaattaaa aggaagacta tttaaaacag ggtgtgcata actataacat
      541 gtgaagtagt tattttgaac tttaactttt aaaaacttta aaaataatct tatttgttct
      601 tctaaacaat catctatact catataatca ctccactaat taatctcccc aaaaagacac
      661 agcattaaga gatataccaa cacaacagtg caaataactc tacatcttcc ttcgcaacct
      721 tatttcaata aaccataaaa attatttttt aatagaaact gaaaattaaa actagaaaaa
      781 gtagttttac cgaatgtaag agcatcatca ccatcaatat agaaatacat tcttttccag
      841 ttacatttaa tttttaaaca aaattctccc caaaaaggaa ctttttctag tgaaaacttg
      901 gtaatctctt tgtaattaat tcttgttaaa gaataattaa acacttcatt tttacttgtt
      961 attttttcat tgactataac tagttcatct ggtaaaaaaa ttaaccaaca aacatcgaca
     1021 aacagtaaat gatataatcc atttccacct ccaagcatat tcaatactgg tgaacatgcc
     1081 ttagaaacac gaatatagtt atggtttaaa tagatatttt ctttaaacat tgcttgttta
     1141 aaagccatat gctcttgttc taaacttttc ataaactttt actctaatgc acagctagca
     1201 gcagctgctg caattcctgc tacaatattt accttagcta actctttagc tgcagctttt
     1261 cctaattgtt tttctgcaat ttttaataat aaatccgcag ctttttcaaa attaaaagct
     1321 tttaatgctt caataaaatc tccctgcgta cctatagccc aagccactcc tcctccagga
     1381 agagaattaa tggctacaca tccagcatat cgtttccaat caactttctc cattctagga
     1441 acaataatat gttcataaaa gactcttgct gctcttcctt gttcgttatc ttgactgatt
     1501 cgttcacgaa tagcaccaaa atcctttata tggtatacac catgttcgtc taaaaaacca
     1561 actgtatcaa aataaaaagt taattcatct actaatttat tctttatttc taaatcaact
     1621 gtattcactt tttctgtggc atacacactt gttccagtac taacaaaagt acttaaacct
     1681 aataagaaaa cacctaaaac accaaatatt ttaattattc ttttcataat ttctcctcca
     1741 taaaaaataa gttacttttt aaatatcttg tttatgaatt cattccccaa aggcctttta
     1801 agattgatac agcagtccgt tcacttcttt tagacttgtc atacaagtct tttaaatgat
     1861 cccatatcag actagtttgt ctatctagct tcaatccatt aaacgctaat gaacggttca
     1921 ttttttctat tactttagat aaagctacag aaatttttat accttcacaa cgcgcatctt
     1981 ggatttcaat aatagtatca tcaattagtt tttgaatttc ttgcctatca acatattcaa
     2041 tcttagaaag agaatctttt aaagctacta atgattcaac gatttctttt ttctcttcag
     2101 acattttctt cctccttcta gtgacccgta aagccatgta gagcagagtc cattgaacca
     2161 gtaattacac tatctacaac gccattccaa tttactctac attttccatt tttcagattt
     2221 gtcggtccat acggatagcc tttttgacat tcaatgttcg gattatacca gataggatta
     2281 taccattcag ccgcagaagc agattcagtc gatacaaaca atcccacacc aacaaatcca
     2341 atacacatca cactatagcc aattagtttt ttcatctcgt atccatctcc taaaaaatag
     2401 aatagttctt atagttattc ataagacata ttttttccgt ttttgtttta tcctttctgt
     2461 cctataacaa cttttttcaa gtaaagaatg acatagtttt tcttaaaaaa acagtctgtt
     2521 tttttctatt tcttcggtgt ataataaata tgagtgttca ttttttattt taatacgcac
     2581 tccgtacata ataaatcttg atcgcatcaa tcttttgagc ttcttgatct actttcaatt
     2641 gaagtaatac aaattgagta accttattca ttgtttcttc tccaaataac aaatattgat
     2701 ccggattctc tatatcttgt gtcacctggt aaagtttccc agttgttttc atttgtacat
     2761 gcgggtctac atcaatacta ttgtctttaa tacaacgatc tgtcatatag tccctcacag
     2821 cttgattgcg tttataaaca tctgtgaaat tactccattt tctagcaaaa tcttttaaga
     2881 aattttttgt catttcatct atttgcattt gttgcgctgt ctcgtcttct aatgaaccaa
     2941 tttgactgtt actttcagta ctttgaagag ttccacttgt ttcttttact gcttcgattg
     3001 tttcttctgt cgttctcaat tgtgtttttt gtttttcaga agaaaaccaa tgacaaccag
     3061 taagacctat caaacataac caactaaata ctaaaattat cccttttttc acgtttctag
     3121 tcactgcctt tcttatttca ccttacgaac cagactacga atttttgttt gattgtacgt
     3181 gcgttgatac cgagccataa tccgtccttg ctcggcattc tgttccacac tagagaaatg
     3241 ttgtccgcca tatgaaacat ctgttataat tccggtgtga ccatagattc caggagacaa
     3301 tgcaccacct gcttgccaat tcaccacatc tcctgcttgt aaatcacttg gttttgggtt
     3361 catgattact tgccaaccat aggttgtcca gttataatct tcccctatta acatcgcaaa
     3421 catgtgtcca gaacccatta actgtgggcc acctaattga gatacatacc atgccgtacc
     3481 accataacat tccccattat ttacggatcg tccaatgatc cgatccaaaa ccttcgcatt
     3541 gcctttactt tttcctgcaa taagcgaagt tttttttgtt ggtaggctat tccaatgctt
     3601 gatcgcaaaa gtatcatatt ccgttaagtt ataggacgtt atgatactca ttgctcctcg
     3661 ataatacgga ctcccttcgg taatacctgc tgccgcatag cctccacgac ctagaatcaa
     3721 taaacaactt ccaggatctt tattgcccac acattgatta aagttgcttt ggctcgccac
     3781 cataaagcca taatagtcat aacaagcttt taagttcgga aacttcatat agaaaccacc
     3841 ttcagaacca ccaattccac gtgcagaacc ttgtgggtag ggacaaccag gaaaccaggt
     3901 aatcccaaaa aagttcgtat cgtttcttcc agattgtgac tgtccataag ctgattcata
     3961 gcctaattga gaaagaatca cactcggtaa taactgatac tttacagctg caccaactgc
     4021 tgcttgcgca tgttcatctg tgattggacc acgtccttta atctctgaaa aaatcggtaa
     4081 tccttcggta tattccccat taaaagcgac agctagacct gtagcttctc ctccatgctc
     4141 agaaagttct cctgaaaaaa agaatggcaa tagaaaaaag ggaagaataa agaaaagaag
     4201 acaacctaga caccctttct tcatttcttc ctcatccttt cttcgataat ggtttttgga
     4261 ctgttcgtcc ttttggtaaa gtaaaatcag ttcctggttt tgcgtccaaa atcggcagtg
     4321 ttttaggatc cttttctatt ttttccgatt gctctgttgg cttatctaaa gaagaaaatg
     4381 caggttgtaa cgattttggt gcggaaatcg acggtttact ttcagacgaa cgttgccccg
     4441 gttgttgcac agaacgattt cctgatagag tggctaccga cgaattagga acagagtatt
     4501 ggttcaataa ctgctgcatt gctggataac tcccattttt cgcctgattt ttcacttggc
     4561 gaatctgttg tgaaagagaa ccagcagctg ttaattgtgg cacgggtact tttccagaag
     4621 gcaacggaat cgattgacca acaaaagcga tacctttatt taacaatggt tttctttgat
     4681 tattcccttg ttgcttactc aattgtgcca atgattgtac ctgttgtttt ggtcttactg
     4741 aattcatgga cgaatttgga atcaaaccat ttgaaagacc agaattccac tctgttttcg
     4801 atttggtaga agacatttca ggctctaccc catgataacg aacatgacga ccactataag
     4861 gcaaggcttg atttgaacgc ccacctagtt caaagggatt aaccacttgc ctccctttta
     4921 ccgattcttg tggtgtacgt ttttgttgaa acttttgaaa tccatgtatc gctgcagaag
     4981 cgagtggatt cgttgctcga tacacattcc ctgctaatcc tgcacctttg attgccaaat
     5041 tttgtccagc agttgatagt tgaccaggca gttgtttcat ttgagacaaa gaatgacttg
     5101 ctatgttttt cgtgttgttt atagtttgat ctgcagctcc taaccctagt ttctttaata
     5161 aatctttccg ttttacaatg agtaaaattg ataggagtgc tttgacaaac atatttccta
     5221 aaaaaccaaa aacagtattc attggaatta aatcgtctac tatgttatag atcaaaaatg
     5281 ccaccaaaat aatcacactg taaaaagatt tttgaaacat caagcccaaa aaggacttta
     5341 aggtattcat gagcatataa tcaaatactg gaacaaaaga agcgagtaat gaaaaaggaa
     5401 tcacaagtaa taaacacaac accaacattt gaaagataaa acgaatcatg ccaatcacta
     5461 caagtggtaa tccaatactt agattaagca atggcaccat agaaccaacg actgctttat
     5521 aaacagcttt agttggttga atatagacac gataagcctt ctttttccct ctatcttctc
     5581 ctgcagcagt ggtcactttt gtctcaatat cagataattc ttcacttgac gcttccttgg
     5641 ctagaaattc ttgtggttca atctctgctt gatacaattc ttctaaattt gttgtcccat
     5701 aatttaacaa tatatacgga tcaacaacag ccgctttata ataaagttgg cgcacttttt
     5761 gaatggcttc cttttgtgtc gttggcacta aaacaccatt gacatctttt gtcgccaatt
     5821 catctagact atttgtagca gaaaaaataa agccttctac ctccacggac aaatcattta
     5881 accctttggt aaaagcttct cctttactag gtacgttact accatagcca aaccaaacca
     5941 ttgaaaaaat gacaacgaag agaaatcgaa taaacaccat ttttgcgtaa tgtttccctc
     6001 taaatatacg aagagtaaat agatataaca catacaagac gacaatcccc gcaccaatag
     6061 gatcgtatat ttttgaataa atagtatccg aaaaagtgaa aaactggtga attaactcgt
     6121 tcattgcagc tccttcatag agcttctcaa tgacaaaatc aaggccttgc caaatcaatt
     6181 tacttaaact aaagaacata ttcgtcatca tttttagaaa atctgtgcca tttgccccaa
     6241 ataacatttt gcctaatgaa aattcactat cagataaata agaagaaaag gtatcaatat
     6301 gaaatttccc aatctcatag atcgattgat ccacctgctt ttctgccgca tacaccaatc
     6361 caccaacaaa aagcagtccg aatccaatcg ttaaaaaaaa caaaacgcac catttctttt
     6421 ccttatgctt tatataagcc attgtttcac ctcatttcta ttgcatgatt aacgtatcct
     6481 gttcttctac ttgagagtga cttgtttttt ccgttgtttg taatgcagcg gtcatttctt
     6541 caaacaaact gtgtacattg agttttccta cacgtccata gggatctaac atccaacact
     6601 gtcctttaat catgtggctc atttcagcta agttcgcttc atttacttcc aaacgtaaat
     6661 gattcaaaat cttttctcgc tcactgtctt catcaaaagc aaagacgaca ccaaaattcc
     6721 ctttttcttt atctgaacct aagtcaccaa taaattgtgt atctaacact aattgattca
     6781 tctgggaacg ccccacacgt gccattgctg caataatact acgtcctgcg ttcgaattat
     6841 taaataccca tgcttctgta aaataaatcg ctgttttctt actactatct cgttttccaa
     6901 acatttcaca aaaacgacct aaactaatca tcaaacacaa agactttcga tccgcatctg
     6961 tatatagttc tggtcggaca ttgtcatttg gtaatttcaa atcttggatc tctaaaatcg
     7021 ttgttttggc attaaagtct aatcccgggt tttctccata agaaaatcct aaacgtaaaa
     7081 tcgaatcttc taccgtcaag cgtaaaaact caccatattc tcgaacctct ttttgttcca
     7141 tttgttctaa atagtcgacc acatgcatca agccaacttg ttgtccggct tctttttgaa
     7201 ctaaaacttc ttggattccc tttaataccg ccccttttaa aaggtgattg gtacctaacg
     7261 gacttaactc atcaatcata tcctgggcta cttgtttcgc atctactcgg gataaacaaa
     7321 caattggatc taatacacca taattacggg gatttgtagc atctaacgtg acataactca
     7381 ttttttgcaa atgttctaca aacaaagggt aatgtttctg ataatacggg tcttgaatga
     7441 gtttcgtaaa ccatttgcgc ttttctcctt tgggatccac aaataaactt tgtacattca
     7501 taaacgaact atacaaaaag agcaagccaa ctaaaaaggt cttcccttta ccggtttttc
     7561 cagttactgc tacatgaggc gcatcaaaca acgcaccttt gattccttcc gcaatggcta
     7621 gaagattcaa aaagacaaaa cgatttgacg cataaattaa ttcttctaat ggtgtttttt
     7681 ctcctcgaga accaccatcc cctaatgtat cttgccgacc aatataccag ccggattcca
     7741 tacctaattc tgatactgct gaaaacaacc attcgccaac tgcttctaca gttgttcggt
     7801 gcttccaatg taactgccgt cccaatggtt gccctaataa acatttatag aacaattctc
     7861 gttgatcatt ttttgcacgg tggatatgga taatcgtatg aagttgttga ataaccgttg
     7921 cactattttg acgaacttct tcaaccgttt gaccgtatac aataaaacat gcgatccaat
     7981 cgacgacagg ttgcccatta tctactcgtt gctttaaatg ttttactaat cctcgaacag
     8041 ttaataagcg ttcactaggt tcttcatcat tttgaaccaa ttccttatgc tctgttttga
     8101 accgttccga tagccaattc agttttaaac caatacctcc ctgtcctttc aagcgtggat
     8161 agtgaatttt cattcgcaat tctgctggga aagaaagatc ctgagcaaat tgaaatagat
     8221 cacaatttct catattttca ggtgtttccc caataggcaa caatgctaaa taactactcc
     8281 ctaactcatc ttctaattga agaaggcctt tatgttcctt cggtatacgt acatacgttc
     8341 cttgattgtc tttcagtact tcccaattat tttgttcttc tactttgtga gccattcctc
     8401 gaatatattg cagacgattg agatacaaca gctctttttt tactaaggga cgaatagaaa
     8461 agctataaag taattgtgtc acttctttta cataaggatc actttcagaa aacaaagttt
     8521 ccaaatcatc tacttctaat ccaaataagc gtgctaaact tcctgctaaa tctaaccaga
     8581 ttagatttat tacatcatga aaactctcca ccgttgcttt ttctttgact ttgattccta
     8641 aaataaatga aggacgatag acttgacctt ctcgttccaa caatgtcact gtttcttttg
     8701 cataatacgc accaattgct tgactatcgg gagaaaatcc ctttcctaat gtgtcaaact
     8761 tcgtccgtaa attcattgtt cgtggataca tcaccaactc cacatcttca taatctgcta
     8821 aaagcaacag taaacgagcc atcatttctt ttatttcctg tgtctttttt ggtgtgtttt
     8881 ttgaaattgt tcgtggaaaa acatgatagt aggcccagat acaatcctct ccatttaaca
     8941 aacaattttt cttgaatgct tttactggtt cttttacaag caccaatgaa tttcacctcc
     9001 caataaaaaa ggaacaacca gttgattgtc cctttttcta atcctctaaa taaacatact
     9061 tattcacaaa aagtaattct tccaatgcat tgtttgatac cttgtcccag ttataataca
     9121 cacaatcacc attcatcata cgaatcatat aacaaaacca atcccataaa taaaaatgca
     9181 ctttttttcc atcaggtttt agttttgcca gtgctaacac acataaatag gggagtccta
     9241 tataaaaaac aactcctgct ggtggaaaaa aagaaatgac ccaaccaata aatgaacgta
     9301 aaaataaata ttcaaagatt acgaccaaca aaaaagttgc tgcattgact aatgcaactg
     9361 gaaatggaag acgccaattg cttgtaatct gttgtacgcg ataaggttcc gcataaacaa
     9421 tcgaataatc ataaggttcc caatccactt cactatcctc ctaatccata aataattttt
     9481 caatgatttt accaaacgca tcaaatacac gtgttggatc atttaagaag aaataaactg
     9541 cggcacctgc tgcgatagat attaatgcat ttcctacttt tgatttcata aaatttcgca
     9601 atcccagata aacaaccacc attattaaaa aatagccacc ttgcgttaat ataaaatcta
     9661 caattccctt cagtgtcatt tccgttcttc tcctttccta tccaaccaat tatgtgttaa
     9721 tttttcaaca taatactgat ttgttttttt cgtaatgaca atcgtcattt gttctttttg
     9781 taaaagtgac gtatcaccat ctcgaaagac gaccgttgct tttacaataa aaggtttttt
     9841 cgtttcttta taacgcaata ctttcatctc tacttgatca actttagctg tttgattgag
     9901 tccttcaggt tctttcatca tgtatgacat atcttttgca ttgtttttcg catacttatc
     9961 taagaaatct tccacaaatc cttcaacatc tttcgcttcc gacgcatcaa ccgtttcgta
    10021 taatgtgtca tcataagtaa tcattcgttg tttttctgta ttctttggta gtgccgtaaa
    10081 ataaggataa gcaataatac gacagccctt gtttgaaata gaaaagggaa gattgagtac
    10141 ttgtgtgatt gtcacacttg tttctttctt actctctgga ttcatcgagc gaatatcata
    10201 agttacttta taactagcag ttggaatggt ttcaatttct tttaatccaa taaacgaagc
    10261 agcagttaat tttcgaacac ctgaaacact tcctggttct tgaataaagt tttgtgcata
    10321 aaactctttt tttaatgttt cttgacgttg ttgttcttgt tcatgattat cgaatgtatt
    10381 catatacaaa gcaacaaaac gattcataaa ggattcaatt tcaggtgtgt acgttaattc
    10441 tgaccgactt tgtacttctt tcttttgtaa ttgttggatt tcatctttca aaaacgacac
    10501 ttgaaccttc gttttcatgg attgaaaaaa tgccaagccc cctgaaaaac aaagaaataa
    10561 aaccaaacac caaatccatt tcactgcttt ttttgaatgg attttctttt gcttcggtac
    10621 tttctctgta taaacttttt ctttccgccg tagttgtcgt tttttcataa gctactcctt
    10681 ctagtatgtt tttactagta cgtacgattg acaacgataa gcttgttttt ctgaaaaata
    10741 gtccattcaa ttaccctcat tttctattcc ttttctcttt cttttatatc caacaaatcc
    10801 agcaatactt gtcaacactc ctcctagaac agaaagtgca acatgtattt cttcaccagt
    10861 ctttggtaag acttttgttt ttccatctgc tgttttgatt tcaatattgc cacttggaag
    10921 tttctttaca ggaatcgaca aatcctttac caaattccct tcactatctt ggattaatgg
    10981 aataccatct tttacaccaa caattgtttg atcacctgct acttttaatg gggtggttgg
    11041 tgttggaata tgggtcaatt cctctacatt actagtgaca atcggtacac tcattccttg
    11101 agaagtattc gtcgtaattt caccgcttgg ggttaccgat actgtgactt cttttgctgg
    11161 aacaataggt ttattttctt ttggaattgc ttctgatttt tcaacaggtt gtttcggttc
    11221 ttctttttgt tcttcactgg tagacgacat cgttggctgt tccgtacttg acgaatcagt
    11281 attcttttca gaagaactgg ttgttcctga ttgttccgga gttgtgacta tacttgaatc
    11341 cgttgttgtt gaactatgca caccttcttc ttcgatcgaa tcagtcggta aagttgattc
    11401 aggaaactct ggttctggtg tcactggctc tttttcaggt ttctctgact gcaattgttc
    11461 aggttctgct ggtttttccg ttgttcctcc atttagctct gtctcaacat ctggtaatcc
    11521 ttcccctggc aatgtttcta caatttctga cttaggtgtt tctacagttt catctgcata
    11581 aaccaatgaa ctccctaatt gaaacgaaaa taatgaaatt cctagtgttg ttacgactgc
    11641 taatgttgtt tttttcatga ttattcctcc aattttttat gcaattcgtg ttttgttcat
    11701 agacgccgca ctaatattat ctgaacgata aaagaactcc cgtccgtttt cttctaaccc
    11761 aacatatgga ttatttttga ataattgaat caatagttca ctggtgttgt attttggata
    11821 gtttgcaaaa aaataatact gtgcttgtcg cccatccgaa aattcaacaa gtaattcata
    11881 acgataccgc ttcaatcctt ttcgttgttt taaaggatct ctataccaac ttgtccatcg
    11941 ttccattcgt ggatagagat agacacgata cacatagaac aatgccatac cacataccac
    12001 caaaaataat aatcgccaca taaaaattcc cccttttctt ctacctttct tgaatcatcg
    12061 aacattcata ccctctatag agatttgaaa aatccatcat caattgttca tataaacgtt
    12121 gttgcaaata gcgattgttt cgaatacata attcttcata gccatcaatc acttgatctt
    12181 ctttttcata gcatcttgta gctctatgac gctcagcttg caagacataa ccaaactctg
    12241 caccatatcg ttctactgtt aataactgtt gactacgcaa taatgcaata taacaatcac
    12301 aaaaatgttc aaccgacatt tgatttttct cacgtttgtg aaataggtat tctttagaaa
    12361 gcacattatt cgttaaacaa tctgataaat agcgataatc tggcggtcgg ttattttcat
    12421 ctgtgaattt ttcccataat aaactgattt ttattcgttc agcggctaga tagtcttgtt
    12481 cgcgtggata aaattcatca atcgcacaaa gttttttatc acctgaataa acaaatccat
    12541 ataatgcgaa acgaactctt ccttttcaat tggctctcct aaaaattcca gtgacatacc
    12601 atcttctcct tcctaaaaaa aataaaaaag cagaaaccga tttggcttct acttttcttt
    12661 ccttttatag ctctttttta cggaagaatc cgcaaatgac attttttcaa aaaatcaatc
    12721 ggcacaagtt cacgaaaact tggagcaatt ttggatagaa tcacttcccc atcttgtaaa
    12781 atacaatcat aaaaatcatc agttacttga tacttacgct tcttacaata attgataaaa
    12841 tagtagcgat cattttctat ttccattcgt tgttctacta agcgcatacg aacatcatca
    12901 tagaaataag ccacaaatcg cccttcaagt tcatgttttt caatgggata ctcttctggt
    12961 aacaatctat tttttcgtcg tttatacatt gctggatacc ttagaagaag ttcatctaaa
    13021 cgtctgtcag tatatggtaa ctgacgaatc aacgattcta atcgttcttt atacagatag
    13081 aataattggc gcaagcaata gtgtaccgcc tctggatttt ctaatactgt tggatcacgt
    13141 gaccaatacg caccaacata aatcataatt tttttcattt agacaatcac accagcaata
    13201 ctaatgaccg atccacaata ggtagaacaa gtggcatagc gaccaatttc ctctaattct
    13261 atctcaatct cttctaaaga aagatatgtc tgttcttctg ttggctggcg actcatttcc
    13321 caagtgacag tcaccaatga aatttcttcc ataagaggtt tttcgctgct aagtgctgcc
    13381 acacagtttt catatatctt cattttatcg cagccttttt taaccgctaa aatcgcaggt
    13441 attggcaatg aaaatcgata aaagcgaatg aattctagcc ttcctttcct tcggctaaat
    13501 tccaactctt cgagttcttg ttcgtatccc ttacgaactt ctaataagcg tgcaatttct
    13561 tgccagttct ctttttcctg ataacgtcca gcttttaccg cttgttcaat ataaggaact
    13621 cgaaaacgaa aataagacaa atcaaccatc ttttcattta atgcttcgat tgcagccttt
    13681 aaatccaaca ggcgcagcca ttctgttgtc gtatatgcat tcatgcaatt ttccctcctg
    13741 acgttcccat ccattctaga tagttttgtt gctcgtcatt tagttcggca ttatcaatcc
    13801 cttcttgtac aaagtctctt cctaaaatac gatctgcttc ttgtaacaac tttaatgtcg
    13861 gagctacttg tttcaaaaac cagcgttctg aacgatctaa tgagaatttt tctggcttta
    13921 ctgtaaaatc caattcttcc atcgaacgaa ataattttgc ccatcgttta tcaacaatcc
    13981 gtgcatcact ttccagtcta tcccaaacaa taagttttgc attaattaaa ccaacgccaa
    14041 tctctcctaa acgtttcggt gaatcaccta aaataaatgc ttctactgct tgtgttgctt
    14101 tctcattggc taaccgtaac tcatatcgat tataaatacc atatttcgtt aatgcttcta
    14161 attctgaaat tttttctcgg ttcgcaattt caaaacgttt ctcataaaaa ttaaaaaaga
    14221 gaggactctt tcgcgatcca aaatacaaag ataatccttc acttcgaaaa tagccatcat
    14281 ctaaaaaaca accgccaata ttctggaatc tcttaaagtc catttctaat aaacctgctt
    14341 tttgtttttc tagtaattca aagagatcga aatgttcttc cgtttccaat ccccaaagtt
    14401 catctaacgc aatatcgaat cggctacatt gcacacgtgt ataaccagct tccaactttc
    14461 cttgattttt ccaaatggaa tatagccatg ttcgccaatc tgtttgttgt tcacgcatga
    14521 cttcctcata ttcccggcaa ccttgtcctg aaaattgcac aagtacattt tctgttacag
    14581 ttcgttttga atgccagaac acccagatat tcccacgaac agaaagacag tcaaaaagat
    14641 acaagcccgt tggacgtgtt tcaaattctg tcagatccat tcgtaataca cgttgaatca
    14701 ctgttttgta gtctgtccca aaaaaggaaa gacgcaaata atcaatcatt gctcgaagat
    14761 ctattccgct tttcatatac aatcgaaagc gttccttgat tcgttgttca aaatcctctg
    14821 ggatttttct tttcccttgt tccatctttc taatgtaatc ttttgagcag cccatcaatt
    14881 gcgcaaatct tgcttgtgat agcggctttt tatctccact tatattatga attgcactta
    14941 acttctttct gtagcttttt agctcaatcg gtgtcatttt ctcgctcctt ttataaaata
    15001 acaaaatcga ttttttttaa ccctttagaa acaccgatat atcaactagt ccccctcgtt
    15061 ttttgggaaa agtagtacaa ctgctgacct tagataatca cttgatacaa aagggtttat
    15121 aaaacttttt tctatgtgtt atgttatttc tgccccccat attatatatc ggggggcttt
    15181 acacaaagag gccgtgcgca agcgcacgac ctctttgtga gctgcacacg ctgctagcga
    15241 gtgcgcatta taggcccatg cttcgcagcc ttaatgcgca ccgccaggca gagtgcggct
    15301 accagactac gtctagagcc ctcccgatag ataataacta caaaattaaa tgatctaatt
    15361 ttgttatttc actatatttt tcaggagaaa aaacgcgata aattgacaaa gttaaatcct
    15421 cttctaaatc tactcgataa tgatacaatc gagtcaatcg ttctatatct tttttcgtta
    15481 gcaatacttg attaataact tttcctgtaa aaacacgttc tttgcgcaaa aagcggtaat
    15541 cagttacttg ttcaatacga ttcaaccaat cataaaacgt tctggaagaa agagaaggat
    15601 taatcattcg tattgctgta gctactctat aataacaact ctctgccatt taacttcacc
    15661 tccttagttg attacaaaat atgcgcatca gaaaaacttg ttccttttgg atcttttgct
    15721 aaatacattt ggcgctctag ttgttcttgt aacaactgat tatcatctcc catcgtttga
    15781 tcttgactat cctcttttac tgtaaaatca tagacaatca tttcagtaaa tttctcaaat
    15841 tctttgataa agtgataatc tttgtaaaca aatggcgcaa aaaactctac aggtacatca
    15901 aatcctggaa aagcatatcc acgaccaggt tgatctttgt tgtaaaatgg cttattttta
    15961 aaatcttctc caaaagtcat taaataacca atgggagaca atcgaccaac agaaatacgg
    16021 aatgttaaat tatcccgaac accacttgga aaatattctg catctggtcg ttgcattgct
    16081 aaaataatgt acatccctgc ttgacgaccc tttaatacta atggctgaat caatttatcg
    16141 actctatttc tcgcttccat atccaacgaa ccataaaaag ctgcccattc atcaaaaaag
    16201 ataaaatgcg gtggtaaccc ataataagca taatttttcc ctggttcata tttaggatct
    16261 ttcatggttt tcatataggc ataccgttga ttcatttctt ttaatgcatt tcgtaaacaa
    16321 ataatcattt cttcatttat tttgtaaaac acatgtcctt tgaatacatc gacgtcacct
    16381 aatgctgcta aatctgccgc ttttccatcg caaatatcta ctgtcccaac acttaaacaa
    16441 gcttgaatta aagaatacaa taaataggtt ttcccaccac cgattgcacc agcaatcaac
    16501 atatttggtg attgatcata tacccaatcc aaagttttca ttaaatgaat acgtccattt
    16561 tctgctatac attgatcaat aggaatccga ccagaatctg aaaggaatac ataattgatt
    16621 cgggcttttt ctcgttcttc acgaattaaa tcacgcaaaa atacttcaga taacaaatga
    16681 cccattttta aaaatcgttc gtgaaaacgc ccaccatcta aaggaaaaga aaccactaat
    16741 tcttgtttct tttgataaaa ataaactttt ggtaatctca ttttttccct tgtttgtttc
    16801 tcactaactt gtttctttgt ataaacaaac cgattgtcaa ttaacattcg aacaagtaac
    16861 tgacgccaat aaacagaggc aaaatatcct tcttttagtc gcgaccaatg catcaaccct
    16921 aaaattaacc cactaaccag caacaaatac aacacaatac ttattccagg aataagatac
    16981 atttgccaat tcgaaaacgt acttaaacgt ggataaaata ctttccaact aaaccaacct
    17041 aacagaatca aaaatggaaa caaaaagaaa ccataatagc aaaaccataa ataacgattg
    17101 aaatagcgaa ttcgccgacc acgatacttg aaaaaatgag ctttcaattg tcacattcct
    17161 cctttctgtt tttacttttc ctgtgctaaa aacttttgac aaaaagcaac taatacagga
    17221 atatcacatt tagtaaaaaa acataatcga aaaagattta atcgattttt ataagggtgt
    17281 gcacacatat tacatattcg tactatacga atccaaatca cttcctgatg atcttgtttc
    17341 cactggtacg gtaatgaaaa acgaacaaga tcacgtaaaa acatacactg cccaataacc
    17401 gcacaaatat atgcatttgt ttgtacaaaa atcgttggat tgtctccaaa atactcaatc
    17461 aattgttgga tattttgata acaagcacaa tctctcaaag tatttgattg gttttatctg
    17521 aattcaaaat ctccccttcc ttctatctta gataaaaaga ataggaagac tccttcctat
    17581 tctttcaaaa aaaccttatt ttttctcttt ttccttagaa tcaacacttg gttgtttttg
    17641 atctaaacct ttttcttgtg attgtttcat attaggatta tttgttggag cttctacttt
    17701 ctctaatcct ttcgcataaa tcactaaatc tgaatattca cgattgccat cttgtgctgg
    17761 aatatatacc acatgatatt ctggatcaat taatttcaca ggatcacgtc gtttaaaacc
    17821 agtcaaatct acatctgctg gaaatttaac tcgtcgtgca atctttggtt catctggatt
    17881 tccaaaaact aattgtgctc gctgtgatcg aatttctccc gtataatcgc ctgtttcatt
    17941 atcaaataca cgatcagact cttctaatgc gccaaagcgt acggctccta aacaagtttc
    18001 atctaatgct tctgtaatgt cataattatt tggaaatttt aatcccatct tacaaaacct
    18061 ccccaatttt tacaccagtt aaaaagacac caacatcttg atagccagtt gttcgacgct
    18121 ctgtttgtcc catatcattt gtttcacgac tattcactcg ttctttacga aaaacaaaat
    18181 aagcttcttc aaatgaaata cgcacctctt tttctggtaa tcgtaatctt ttatcctctt
    18241 cagctgtgag ataaacaaaa aaagcaggct gttcttctcg cgaacactct gcttgtaatt
    18301 gaacatatcg cccagacaca ccattaattt cctttggtgg attaggttcc cgtgaaccca
    18361 ataaaaatac ttgacctaaa aactctttct tcatacgcaa tgctttttct gccataaagt
    18421 ttttcctcct cttaaaaacc aaaataaatt ctaaatattt ctacattaca atcaaacaag
    18481 cggttaaaaa tcctaaccgc ttgttcctca tgactaatct ttggctttac gtactttata
    18541 aagtgttata cctaaagcaa tgactaatac tactaatcca agaaccataa gtatactact
    18601 tgtttgttca cctgtttttg gcaacattgg tacaacagta gctggtttaa cccgttgttc
    18661 tggtttcact ggttgcggtg gttttggcgc ttccacaatt tgaaccgttt gatctttgtt
    18721 tgttagatct acattgtgtt tcgccacttc ttcaaacgtt tctttctcat cttccgtttt
    18781 cgcttggagc cattccgcaa aactcaagcc ttttcctgca agattgcgac cgtccactaa
    18841 gaattcaaat aagaattcca tcatttcttt atcggccgca aagacttttt ccgcatgagt
    18901 gccttcaaat ggtgtgccgt ctggtgttaa gtagccttga acaattaagc gatagatttg
    18961 accaatcgca agaccgtcta ctttgaccca ttcaagcaca gtttgatttg gcaaggccaa
    19021 gatctctttt ccgccatttt tattcgtcgc tttcgtcgta atggttggat tactgatttt
    19081 gatggtttgg tcattgtcgg taatatctgc gtgagaagcc aataatacgc cttctctaaa
    19141 cagatcttca aagaccacga cttctttgcc tcgcaacgca cgtgcgtcta gaataaagtc
    19201 tagattgact gtaccatccg ttgtttctgc cacaaacgtt aaggtacttg tgacttcttt
    19261 tccatctact agcactggtt tttccgttac tttgtccatt agaacaccgt tgactgtgta
    19321 tgtttttcca acgaccaaat cgtggtattt cacagcgtct gtaatggtga ctttcgttaa
    19381 tggattaacc tctttcaagc cagtgggttt atgtgtagcc gttgtacgta cacttgggtt
    19441 agttactcgt accgtttgcc ctttgtcgtt gatgtcttta tggtttgcta gttcttcatc
    19501 atttctaaaa agactttcaa acacgacgat ttcacgacca tttatacctg tcaaatcaaa
    19561 ggtaaatggt acgatcaccg tttcattttc tgctttggca ataaagccaa cactcgtttc
    19621 taccgtattt ccatgcacaa gcaagggttc accggttgct ttatccatta agatcccttt
    19681 gaccaagtat gcttttcctg gaagaatctc ggtaatttca acggtatcgt ttaaggtaaa
    19741 caatcctgtt gggtctgcga ctgtcaatcc gtcttcaaag acagccgtgg tatgaacggt
    19801 tggattattc actttcacac tttgggcttc atcgttgata tccgcatgtg ctgcaacttc
    19861 aatgccatca cggaaaagtt tttcaaaaac aacgatcgtt tgacctttca gaacactagc
    19921 attaaccaca aattcaaggt cttttgtgcc attgactttg tctgcaataa aggccaaggt
    19981 acttgtgatt tctttgccat cttgtaaaat tggcgcattc gttgccttgt tcatcaatac
    20041 tcctgaaatg gtgtattctt ttccgacaaa caagttggta taagaaacac tgtctttaat
    20101 gacgatcgtt tctaatggat caaccacttt ttcaccatcg gctttatttg acgcttgtgt
    20161 ttctagtttt ggtggaatca agtaattacg tacagaaacc actaacgttt ccccatcatc
    20221 tggcgcattt tgtttgaatg caaccgtgaa tgcttgttta tctgtatttg gttggaaact
    20281 ttctaatggg gcattttcca agaagtaata gtcatcggca actagacctt tcactaaaat
    20341 ttccccgttt tgatcagtgg tatattcacc aattgcttga tttttaccgt tatacaaggt
    20401 aaagttcaca cctgcgagcg gcgttttctt cttgttccat tcttcatact tgaataattt
    20461 tacacttttc cgtgcttgac ggttcgcaaa atggatcact ttgatcggtt ctgtatcatt
    20521 ggtaatcgag aacactaatg ggttttgatt caaaacgtaa tttacggggg ctttgatttc
    20581 ttccaaacga tagcgatctt ttccataagt aagtgtaccg ttagttagga aaaagccttt
    20641 ttcattggtt ttaaagatat ccgttgtttc tgtatcattt ggaatctcca ttgtgacata
    20701 acgattggct aaagtatcaa aaatcttgaa ttctgtatta gcaagtggaa tcacttttcc
    20761 tgtttcttga tccactttca ccactttaag acgttgttca attactttat cttctaaaac
    20821 ataatggaaa gtttgcccat tttcagaaat tgtgacttca agtggttcaa atagtttata
    20881 cccttctttt cctttggttt cagaaagaat gtacgtatca aagacaacgg tatttttacc
    20941 aaaagtagca taaccatcat tgtctgtcac aacggttttc accactttgc cagttgtttt
    21001 tgacgtcaaa gtgatttcaa tgccagctaa ttttggtttg ttatctggat tcgttgaatc
    21061 gactaaatct ttattagcaa ctttcactaa atcaatggtt cctttgatca cttgatcttt
    21121 tgcggtcgca ttgacagttt ttccaggttc aacaacaatt tcaattgctt catcagaaag
    21181 cacataacct tttggcgctt tgatttcttt taaaacatat ttgccaggat caagatcttt
    21241 aatttctgcc tgtactttgc cgttaatttt tttcaaggta atttctttaa tctttttccc
    21301 atctgcttga tacaaaccat agaccgcatt ttctaaagaa gctgcacctt gtgctttcac
    21361 accagtttcc gcatcttctt taaacacacg aatgtttcct tgttttagaa tttgtaaatt
    21421 aataacagac gcattaggtt ctgttggatg taacacacca attgtttgag ctcctggacg
    21481 acgataggct aatggcgcac cttgaacttt aattttattc aattgaattt ttgctttatc
    21541 cggtgcctct tttgtgactg taatccatac ttcattgccg tgaatagata cattgacacc
    21601 ttttggtttt tgaatgtctt tcacatattt caaaaaatca tttttattct ttactttcaa
    21661 cgtttcgcca atttttaccg tatgtgtttg attattaaaa gttgcttttt caccaaagtt
    21721 attaatcaaa cgttgaattt ctgcttttcg acgatcataa tcaacacgga tatttttgat
    21781 tgggattttt gtgcccaaag cttcccaaat cataaattgt gccgcaatgt attgttcatc
    21841 ggatttgtct ttatttgtga catatcctaa ataagagatc aagcttgttt ttttccgtac
    21901 atttgaattt ttcaaatagt ttttcaaagc agtagctgta taaacttgac cttctgcggc
    21961 tgagacacca ggctcaatac aaaacgccac ttgtccatta atctcaaaac gaaccatata
    22021 atttaaagtg aagtttagat tgttcggact agaagtaaca ccaatatttt cgccccaatc
    22081 aattttccga ccacctaaaa cagctgcatt ggttgcacgg cgaacacgaa ccgaacgatt
    22141 ggttggtact gttactaaca gtgtaggtgt acctgttttt ggaatgttct gctctttttt
    22201 cttttgattt tcaatactag ccgtagattc tttgtttggt ttcgttggtt gattttgact
    22261 agctgcggtt gaagagattg ttttttcaac gttttcctca ttttttcctt ctttgtcagc
    22321 agtagccgct ggttgttcca ctgtttcttc tattcgtttc ttttcttccg cttgtttctc
    22381 agaagactct ggctgcacag aaggcgttgc ttcggcagct ttttctcctt ctagagtgga
    22441 cgtttctgaa gcaggggttg taattgaggc agtttcttca actggttgtg aagtaccagg
    22501 actattccct gggtgttgtg tttccccaat cgtctgatcg agagtcgttt tggaattgac
    22561 cgttggcgct tctgtcacag gggcctgttc aacagtggct tcaccagctg ggaccccggc
    22621 ttctgctggt tggacttctg tcacttcaga agcgacttgg gcaatggctt ccatcgtagg
    22681 ttgtatagcc gtcgtttctt ctgcatgtgc ttgagcagtt cccaccaaga aacagaattg
    22741 agaaaaagcc accaatgcga gtgctttcaa ccaattcatt ttcgtttttg catacacaag
    22801 aattcctcct tttcctgaga attttatata ataaaacaaa acgccgttac caaaagtgat
    22861 aacgacgtga actgtctctt ctatttttat ctggcgctct tatctgaaca catttcatca
    22921 atgattttac cctcccacta taattacaga acaaaaaaag aacctgcttc gtggaaatca
    22981 actgtccatt tcccaattgg aataccagct tttttggcaa cttcaccatc accaaatctc
    23041 atgccatacc aattactacc ttcatcctta atttctttcc aaccatatga catagcttca
    23101 tctaatgaat caaaaatcat gacactattg ccaattgtta caggatctgg cttaagatct
    23161 tctggaagag aaatatcaat cataccataa tgatccgatt cctcagtatt attgtcatta
    23221 ctagctactg gtttggttgt tctactggct tattttgata caacaagtac tcttattctt
    23281 cttttacttc tccgttttca atttcccatt caaaccactc cgcaaagtct tgcttaaaac
    23341 catctactcg atacctattt tgtaaatagg catcttctga cgtcgtagga tctaaatagg
    23401 taggtttatt ctctggacga gcatagacac ctacccatgt ttcgtaatct ccttcaaaat
    23461 aactatctac caaatagcct ttttttctaa gaatctcccg aacttgattt ttaattattg
    23521 atacttgttc aaaaccatca ctcattctat cacctcaaat aactctagat tctcataaat
    23581 attcccgaca atttcaatcc tatcaatgat ctctaacaaa tcgctactta tgcttcccca
    23641 ttcgtataag aatttccctt ccgcaaaata aattttccat aaacttcttc atgatcgtcc
    23701 caaccaatat cactctcgaa aaattccacg ttgttcttat cttttagctc agtaaatgcc
    23761 ataagttcaa catgttttcc aacctcgaaa caatcacacc acccatcaac cgttacacgt
    23821 gtgccttcaa tacctaagaa actttgtcct cgtgtattaa acactaaaac tttccgcatt
    23881 ttttgctcat taattaatca cgcgcgaaat ctaggaatca caacttattc gcttcttctt
    23941 tataaataaa aaaactccaa taatcggaaa ttactatctt ttcctcattc cttttttaaa
    24001 tcttcatact tcttcttatc tttgtcctaa cctaataaca caaattttga agaaaaactc
    24061 agtaaaacct gattcttttt ttatcttcac aacaaccaac tttttcttca gatcctttta
    24121 ctagaacttg tatacaaaaa ttgttgaatg ctgatataat ttgcggctac ttgaaaaaac
    24181 gatagatgta aaaagataaa tagaatcatt ttttattcta tatcagtcta tgattcattg
    24241 cttatcaatc acccaaccca ttcgatgccg ttttttttag agaaatttaa aaaagaccgt
    24301 acgaatctcg cattatccga ttacttttcc agaaaagatc cccaattctg tataaataac
    24361 cctatcttct tgttggtttc attttctcat ataagaattc agtcaatcta cccactatga
    24421 ccattactac gtccaacaca actgacatat taaccacgta agtattccct cacagatctc
    24481 cgaatctcca tggtatggat tcccatcctg acgctgcaga ggacaacata tttcatgtgg
    24541 ctctgtaccc ttttgatttg gaaacactac aaatcaatac tcatctataa cttatcaagc
    24601 gaactttcgc taggcttcta gtgattacta aattgttgga taacatacat agcccatttt
    24661 atctagctgt tattcataac agttagttct tgtattactt cttccgcttg tgttgccatt
    24721 gagcttgtca aaaagttact tagaaaaaag ctgccagtaa agcaactttc aacaaaccct
    24781 ttttgtttct tatcatatca agaaacctcc ttttataaat tttttctata ataaaaaacg
    24841 tcgataactt aataagtatc gacgtaaact atttttatat cccgtaaatc aaaaattcta
    24901 aagaaagatt tcctatataa gcatattgga taattcgatt agtattgggt atatctctac
    24961 cattctcata cctatctatg gaccattttg aaattgaagg agaaaaaagc tttccaaact
    25021 cctccctttc tagataataa tctttcctaa ttttcctcat ccttaatccc atactaaaag
    25081 aatttatctt tttaaaatcg ctaactcttt catcttttgg aagtatattc ttataatctt
    25141 ctccgtacat aaggtaagga acagtgacct taccaagata ggcaatattc attagttttt
    25201 caatatctgg aatattaata cctttttccc atctacttat aacatttttg cctgtataga
    25261 tggtaaagtt tttaccaaat ttttcaagcg tctcttcccg gttctttcgg acttctcgta
    25321 ttctttttcc cataacaatt ttatcgtctt ttgataaact aaatatctca ctatcacgat
    25381 taatctcctt caaaatttct ttttcattac ttaatagaaa aatatcgatt ttttttgcat
    25441 ttcttttgac ggatttctct ttctatttaa cacataactg agtaatactt cactaattcc
    25501 tatattatca gctaactctt tgttaagtaa cttattcata tctctgtatg caattagttt
    25561 accgatgaga atctcaatct tatcttcctc atcttttatt agagtttcta tattcagaat
    25621 cttgcttaga gtattatcta cgtctttagg gatttttctt gacccttttt catatttaac
    25681 taaagtactt tgtgaaatac ctgctaaatc agctaacttt ttttgagata gttttttctc
    25741 ttttcttaga gatttaagaa cccttccact tgaattatca accatgaaaa aagcacctcc
    25801 tatgagatgc tttcatcata tactgacagc taaaaatctt caagaaaaat attccaattt
    25861 tgttaatttt agtatcgccg tgataaccaa atgacagttt aaaatctttt atacagataa
    25921 tcctcagtgt tgcatctact caaaaaggtt tatttaatag gtttttcgat tgattataag
    25981 ttatttcaca ctatcttgct catccatttt cagaacggcc atgaaggctt cttgcggaac
    26041 ttcgacggag ccgatttgtt tcatccgctt cttcccttct ttttgtttct ctaagagttt
    26101 acgtttacga gaaacatctc caccataaca tttagccaag acgtttttac gcaaggcttt
    26161 gatgtctgaa cgggcaacga ttttttgtcc aatagctgct tggattggca cctcaaattg
    26221 ttggcgtggg attagttttt tcagtttttc aacgattgct tttccacgtt cataggcaaa
    26281 gtctcggtga acgataaagc ttaatgcatc gactttttct ccattcagca agatgtccat
    26341 cttcactaac ttgctttttt gataaccaga catttcataa tccaatgagg catagccttt
    26401 cgtgcttgat ttcaatttgt cgaaaaagtc aaagacaatt tcagaaagcg gaatattata
    26461 gacgacattt actcggtaat catctaaata atccatcgta atgaattctc cccgttttcg
    26521 ttgagaaagt tccattactg caccgacaaa atcatttggg accatgatct gtgctttgac
    26581 gaatggttct tctacatcct gaatcgttac aggttcagga aaatctgccg ggttatctac
    26641 tgtagcagtc gtaccatccg ttttattaac atggtaaata acggatggtg cagtcgtgat
    26701 caattctaga ttgaattccc gttccaagcg ttcttggacg acatccatat gaagaagtcc
    26761 gaggaatcca cagcggaacc caaaacctaa tgcttgagag gtttctggtt cgaattgtaa
    26821 agcagcatca ttcaattgca gtttttctaa agcttcccgc aaatcgttgt aacgagaagt
    26881 atcaattgga taaagaccac aataaaccat cggattcatt tttcggtaac ctggtaaagc
    26941 ttcagcggca ggattgtctg ctaacgttac tgtatcccct actcgtgtat cttggacagt
    27001 tttgatactt gctgtgatat aaccgacatc accgaccatc aaaaagtctc tagcaactgc
    27061 ttttggcgag aagaccccga cctctgtaac atcaaatgtt ttgccattac tcatcagttt
    27121 gattttgtct ccgggtttga ccataccgtc cgtgatccgg acatttagaa caacaccgcg
    27181 gtaactatca taaatagagt caaagatcaa agcttttagc ggtgcatcta agtctccact
    27241 tggtgcagga acatattcca cgatctgctc gagaatatct tcaatcccaa tccctgattt
    27301 ggcactggct aatacagctt cactagcatc gattccgatt acgtcttcga tttcttgacg
    27361 gacacgttcg ggatctgctg cgggtaggtc gattttattg atgactggca ggatctccaa
    27421 atcattgtct aacgctaaat aaacattggc caatgtttgt gcttcgattc cttgtgctgc
    27481 atcgaccact aaaacggcac cctcacaggc agcaaggctt cgagagactt cataggtgaa
    27541 gtcgacgtgc cctggggtgt caatcaagtg aaagatatat gtttctccat ttttcgctgt
    27601 gtaattgagt tctacagcat ttaatttaat cgtgattcca cgttcacgtt ccaaatccat
    27661 cgagtctaac agctgatctt gcatttcgcg agacgtcaca gtgtgcgtca tttctaaaat
    27721 acgatcggcc aatgtagatt tcccatggtc gatatgagcg atgatcgaaa aattacgaat
    27781 ctgctcttgg cgttctttca ttttctttat atccatagcg tctcctcttt tcatataatc
    27841 agcactttcc attataccaa cgaaaagcag taatttaaag agtttttaat tagtcagcta
    27901 tagaagtttt ttttgtagaa ttttgctata ctgttatcaa gctaataaat ggagatgaaa
    27961 aaatggatca aaaagatttt agagaaaatc ttgaattaaa accggtcact caagaacata
    28021 tcgatcaatt caatgaattg ctttcctatg tgttccaagt gactgaagca gacatcgagg
    28081 aaagcgggtt tgagaataaa cgagctttta tccgttccaa aaaaccgatt ttggaagtat
    28141 cgaaagtgtt cggatggttc cacgaaaata aactgatctc tcaaatcgct atctatcctt
    28201 gtgaagtcaa tattcacggt gcacgttata aaatgggtgg tgtgactgga gttggaactt
    28261 atccagaata tgctaaccat ggattgatgc aagatctaat tatcgttgca ttggataata
    28321 tgcgcaagaa taagcaatgg atctcttatt tgtatcctta cagtatcccc tactatcggc
    28381 gtaaaggatg ggaaatcatg tctgataaac tttcctttaa aatcagagat acacagctac
    28441 ctaaacaaat cccagtaagt ggtatggttg agcgattacc agttgatcat ccagacgtat
    28501 tttccgttta tgacaaattt gcccaacaaa accatggtgc tttattccgc agtgattttc
    28561 attgggaaga gtattggcgt ttcgaaaatg aagatgagcg cacagccgct gtctactacg
    28621 atagcaacca tgaaccaaga ggtgttctgt tctactgggt agctgaagaa atatttcacg
    28681 taaaagaaat gttctatctt gatcaagaag ctcgaaatgg cttatggaac tttatttccg
    28741 cacatttttc catgatctac tgggtacatg gagatattta taaaaatgag cctttagcat
    28801 ttttgattga agatagtcag atcaaagagc agatcgaacc atattttatg gctcggatcg
    28861 ttgatgtaaa agaattcctt caacggtttc cgtttgttgg aacagctgat gcttttcatt
    28921 tcatcattga agatcctgtc gctccttgga ataatggtgt cttcgcttta acatgggatg
    28981 aacagggaca agtccgtgtc ttaaatgaac ctataggaaa atccgttcga ctggatatcc
    29041 aaacattgac ttgcttgatg atgaattatc gccgtgcttc ttatttggca agaatcgaac
    29101 ggttagaaac agatgaagaa acactgaagt cactagagcg tatcattcca aacatggaag
    29161 cttactttag cgattatttt tgataaaaag gatgatgttt agatgaaaat ttattttgcc
    29221 gcaccaatgt ttgctaaaag tgatttgtta tataaccaac agttagttaa agaaatcaga
    29281 gagctctctt ctgagctttc gatctatctt cctcaagaaa atgaagcaat caatgacaaa
    29341 actgcttatg cagacagtcg aatgattgct cttgctgaca cagaaaaagt gttggctagt
    29401 gatttaatga ttgctttact agatgggctg acgatcgatg ccggggtagc atcggaaatc
    29461 ggtgtggctt atgcgaaagg catcccagtg atcggcttat atactgatac tcgacagcaa
    29521 ggcggaaccc acccgaaaaa aatcgctgca ctacaagaaa cagctgaaaa tcaatttcac
    29581 tacctgaact tatatacaat aggtttgatc aaattgaacg gaaaagtggt ttcttcagaa
    29641 attgagttgc tttcagaagt aaaaagattt ttagatggag ggactttcag tgattaaaga
    29701 aatcaaaaag gtgcaaatag ctttacttgc ttttggtgta ttcgttgtct gctataattt
    29761 ttatgaattt atcacacaaa aatattcgac ttctcaagga atcacattta tcgtggaatc
    29821 tttattaggg attgcattga ttttcatgcc acaagtcatt ttgaaagttt tcaaacttaa
    29881 aataccagca gcaatcgttt tgttttactg gtttttcttg ttcatctctg tctttttagg
    29941 cacagggatg catttgatca gtatcatctc tttttgggac aaaatcttac atgcggtgag
    30001 tccaatggtc ctgacagctc ttggctatgg attgattggt tacttgatga aagatgctga
    30061 gatatcaaaa accagtcctt ggttgttcct cttattcggc tttgcgtttg ctggactttg
    30121 cggcgtgttt tgggagttct gggaattctt atgcgaccag ttcttaggca tgaatcttca
    30181 acgttttgct gcttctgatg gcacactgtt tgtcggacga gcagcattga tggatacgat
    30241 gggcgacttg ctgacaaata cgataggtgc tgccttaatg ggattgtttg catggtctca
    30301 aagtaaaaaa gatgagcgtt actttgaaag ttataaatta gaaaaagtga aaaataccta
    30361 ataaaagagg ttgtgacaga agcgggcagc ttcaagaaat ttcggcttat ttttcgaaga
    30421 aactacttct ggaacactgt ttatttgaaa aaaggaggtc tgacactagt tgtgtcagac
    30481 cccttttttc ttttatagtc tcaatggaag attagacgat ttcttctagc tcttctgttt
    30541 tgaaatgaag ttgtccatca agtaaagtaa tcgttacttt tgatttaggc ataacatgtc
    30601 ctgcaacgat ttctttagct agtggtgttt ctacttcctt agtgataaat cgtttcaatg
    30661 gtcttgcacc atatgctggt tcataagcat tttcagcaat ccacgttttg gcttcatcac
    30721 tgattgtcag caggatttct tgatgttcca gacgttgtgc caattgcgcc accattttgt
    30781 caacgattcc tttcacgttg tctaggctca atggtgtaaa taaaatcgtg tcgtcgattc
    30841 ggttcaagaa ttctggtttg aagtttccac gtaatagcgt gttgacttgt tcagccactg
    30901 cttctggaat cgttccatca gcagtcacgc cttccagcag taactgtgaa ccgatattgc
    30961 tggtcatgat cagaaccgta tttttgaaat ctactactcg cccttttgaa tcagtcaagc
    31021 gtccgtcatc taggacttgt aacaagatat tgaagacatc gggatgcgct ttttcgattt
    31081 catccaacaa gacgattgta taaggatttc gtctgacagc ttccgttaat tgaccacctt
    31141 cttcgtaacc tacatagcct ggaggagctc cgactagacg agacacagca tgtttttcca
    31201 tatattcact catatcgatc cgtaccatat gatcttctga gtcaaacaag ttctcagcta
    31261 acgctttcgc taattcggtt tttccgacac cagttggtcc taggaaaagg aaagaaccta
    31321 gaggacgatt tgggtcttgc aatccagccc gtgaacgaat taccgcgtca ctgaccgcat
    31381 caactgcttc atcttgacca atcactcgtt tatgaagtgt ttcattcaat ttaattagtt
    31441 tttctcgttc accttctaca agtttcgtta cggggatacc cgttaatctg ccaacgactt
    31501 gagcaatctc attttcggta acggactctt gaaccatctt aatctcgctg tcttttgctt
    31561 tagcttctaa ttccttcaat tctttttcta attgaggtat cgtaccatga cgcaatacag
    31621 cagcgcgttc taaatcatag ttattttctg catcttctaa ttcgtgtttt gctttatcga
    31681 tttcagcacg ttttgctgaa acagaattga cttcttcttt ttctgtttcc cactgcatct
    31741 tcatcgcatt tgcttcttca cgaagttcag ctaattcttc ttgcaaattt ttcaagcgtt
    31801 ttttacttgc atcatctgat tcttttttca gtgcagcttc ttcgatttcc aactgcatca
    31861 aacgtctagt aacttgatct aattccgttg gcatagagtt catttctacc cgaatcgttg
    31921 cactggcttc atcgatcaaa tcgattgctt tgtccggtaa gaaccgatca gtaatataac
    31981 ggtcagataa agtagctgct gctactaatg cgttgtcatg gatgttaaca ccatggtgga
    32041 tctcaaaacg ttcttttaat ccacgcagaa tactgatcgt atcttccact gttggttctt
    32101 tgactaatac cttttgaaag cgacgttcca acgctttgtc tttttccata tattgacggt
    32161 actcatctaa agttgtggca ccgatcaaat gtaattcacc gcgagcaagc attggtttca
    32221 acagatttcc ggcatccata cttccttctg tttttcctgc ccctacgata ttgtggattt
    32281 catcgatgaa caagaggata cgtccatcac tttttttgac ttctttcaag actgctttta
    32341 gtctttcttc aaattcacca cggaattttg ctccagcgat caatgcaccc atatctaatg
    32401 aaaaaatcgt tttatctttt aaattttcag gcacatcttt gcgtacgata cgttgagcaa
    32461 gcccttcaac gattgccgtt ttccctacac cgggttctcc tatcagaaca ggattatttt
    32521 ttgtttttcg tgaaagaata cggatgacat cacggatttc ttcatcacga ccgatgattg
    32581 gatccatttt tccgcttttg acttgctgta ctaaatcaac gccgtatttt tctaatgctt
    32641 tgtattgttc ttcttggttt tgtgaggtca ctctttctcc tcctctcatc tcttcgatgt
    32701 ttttacgcaa ttctttttca gataatccgt ttttattcaa ataaacagtt aacggataat
    32761 tttttaattt catcaacgct aaaataacga cttccgttga aagaaactcg tcgccgaagc
    32821 tttcacgtat ttggtctgct tcattcaata atcgaaagaa attctggctg aggttttgtc
    32881 catattggac gtttccacca gaaacaactg gatactcatc cagtagacga tcaatttcat
    32941 gttcaaaaga ttcgacatcc aatccggcat ccgtatagaa atttcgacca aaatgattag
    33001 attgtaaaaa gattttccat acatgggcaa tgtcgatatc ttgatgtttt cgagtgaccg
    33061 caatctgttg agcttcagca atcgcttctt gaagcgtcgt agtcattttc tcaatattca
    33121 tacaggtata ccctcctaat taaaggtaag atcacttatt tctttgatct ttacaaaaac
    33181 tattatatcg ctttggtcaa ttttggtcaa aatatacaca tagttttttg ac
//
LOCUS       DAZBWR010000018        30682 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000018 DAZBWR010000000
VERSION     DAZBWR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.018"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHGT5_000795"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHGT5_000795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN7216803.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHGT5_000796"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHGT5_000796"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN7216804.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="ACHGT5_000797"
     CDS             8128..9096
                     /locus_tag="ACHGT5_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7216805.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="ACHGT5_000798"
     CDS             9114..10019
                     /locus_tag="ACHGT5_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216806.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="ACHGT5_000799"
     CDS             10016..10822
                     /locus_tag="ACHGT5_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216807.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="ACHGT5_000800"
     CDS             10979..11947
                     /locus_tag="ACHGT5_000800"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN7216808.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="ACHGT5_000801"
     CDS             complement(12264..12788)
                     /locus_tag="ACHGT5_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN7216809.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..14829
                     /gene="scm"
                     /locus_tag="ACHGT5_000802"
     CDS             13075..14829
                     /gene="scm"
                     /locus_tag="ACHGT5_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN7216810.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
     gene            complement(14984..15970)
                     /locus_tag="ACHGT5_000803"
     CDS             complement(14984..15970)
                     /locus_tag="ACHGT5_000803"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN7216811.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15993..16559)
                     /locus_tag="ACHGT5_000804"
     CDS             complement(15993..16559)
                     /locus_tag="ACHGT5_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN7216812.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16573..16989)
                     /locus_tag="ACHGT5_000805"
     CDS             complement(16573..16989)
                     /locus_tag="ACHGT5_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN7216813.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17446..19380)
                     /locus_tag="ACHGT5_000806"
     CDS             complement(17446..19380)
                     /locus_tag="ACHGT5_000806"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN7216814.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19682..20617
                     /gene="pplA"
                     /locus_tag="ACHGT5_000807"
     CDS             19682..20617
                     /gene="pplA"
                     /locus_tag="ACHGT5_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFN7216815.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20707..21810
                     /locus_tag="ACHGT5_000808"
     CDS             20707..21810
                     /locus_tag="ACHGT5_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN7216816.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21823..22776
                     /gene="menA"
                     /locus_tag="ACHGT5_000809"
     CDS             21823..22776
                     /gene="menA"
                     /locus_tag="ACHGT5_000809"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN7216817.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23590..24936)
                     /gene="gor"
                     /locus_tag="ACHGT5_000810"
     CDS             complement(23590..24936)
                     /gene="gor"
                     /locus_tag="ACHGT5_000810"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN7216818.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25070..26323)
                     /locus_tag="ACHGT5_000811"
     CDS             complement(25070..26323)
                     /locus_tag="ACHGT5_000811"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN7216819.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26329..27252)
                     /locus_tag="ACHGT5_000812"
     CDS             complement(26329..27252)
                     /locus_tag="ACHGT5_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN7216820.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27473..29965)
                     /locus_tag="ACHGT5_000813"
     CDS             complement(27473..29965)
                     /locus_tag="ACHGT5_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN7216821.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(29972..30439)
                     /locus_tag="ACHGT5_000814"
     CDS             complement(29972..30439)
                     /locus_tag="ACHGT5_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN7216822.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(30575..30648)
                     /locus_tag="ACHGT5_000815"
     tRNA            complement(30575..30648)
                     /locus_tag="ACHGT5_000815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30612..30614),aa:Arg,seq:acg)
BASE COUNT         8885 a         6529 c         5729 g         9539 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tcttttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    14161 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    14221 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    14281 tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct
    14341 accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc
    14401 acaagtgata gcacggacac gtcagcttca agtgacagta cagatactac ctctaccaca
    14461 agtgatagct cagattcatc taccactaca agtgatagca cggacacgtc agcttcaagt
    14521 gacagtacag atacatcctc taccacaagt gatagctcag attcatcaac ttcaagcgat
    14581 agtacagaca cgtcgtctac cacaagcgat acacgtcatg caagtgtctc cttctcaaaa
    14641 acaagttcgg ataacaggac gaactttaac ttaactaata aaacaagcaa tgacaatgga
    14701 aacaaatcaa aaagagcact tcctaaaact ggaagtcaat cgaataattg gataacactc
    14761 gcaggcgtta tattacttgt aatcggttta agaatcactt ttatgagtta taagaaatcg
    14821 aaaaggtaaa aaaatattca agataaaaaa gagcaacgtc gctaacggac gttgctcttt
    14881 atttttgctt tttatcataa atacaagaac tacaattcaa tttgtcaaaa aacacgtaga
    14941 tttccttctg agaaatttat ttacgtatcg aacctttccc tattcagtct ttgcgatcca
    15001 aaatcagttg ggtaagggaa aagagattct cttttgttcg aagagaggtg gcaggtaatt
    15061 tctttatttc cttcaaagcc ttttctgtgt aagaggccgc taacttccta gcttcttcca
    15121 cgcctcctgt atgttcaacg attttgcgta cggcgtcgac atcttcttct gtcattgccg
    15181 ctttcttctc taaataaggt agtagctttg gtttgttttt ttgcaaagag tagatcaacg
    15241 gtaaagaata gaccccttga cgcatgtctt ctagtacagg tttgccgatt tcctggctct
    15301 cttttgtata atctaaaata tcatcgataa tctgaaaggc catcccgata gaaagtccga
    15361 tatctcctgc tttttttgcg aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac
    15421 aacttaattg gaacagttca gctgtcttac ctgaaatatt ttccaaatat tcttttacag
    15481 aaagttcata attatagcga tcatccatct gccctaattc gccattcaat actttttcca
    15541 tactgcgaga attcaattgg agacttttca aggaagtgga gtaatcagac aatagtttaa
    15601 aacagcagac aaataaatag tctcctgcat agacagaaac gacattacca aactgactac
    15661 ggatattagg ctgaccacgt cgcagatccg cttcatcaac gatatcatca tggatcagtg
    15721 tggccgtatg gagcatttcg atagaagctg ccaaagcgat ggctttgtct cgatcttgct
    15781 ctggtccaaa ttgtgagaac aatagctgat aggcaggacg caacagcttt ccgccggcat
    15841 ggatcgtttc tttgattgct tgttcgacag gtttgttttt caggcggaca gtttcttcca
    15901 tccgttttag tgtttctgtc aattctttac ttaaagcagg atagttttcc cacattggat
    15961 gtagcttcat aaaaaaactc cttgtgtgac gattattttt tggtaaacat tgggctccat
    16021 ttttgtttgg ttccctgctc ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt
    16081 aaaagataat tcgctgcgat cccgattgca atccctgata aaatccctaa aactgaaagg
    16141 actggcaagt acaacatgac tgtccatgat tgtgcaatga aagaagccat caaaagctgt
    16201 ccgacattat gcatgaaacc gccggccgca ctaattccga tgatactgac acgttttggt
    16261 cctaattgct tgaccaataa cattccaaaa taactaagca aagcaccact catactataa
    16321 aggaatgtgg aaatcgttcc gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt
    16381 aagaagctgt cttgtttttt catggtaaaa atggcaacga tcgtgattag attggctaag
    16441 cctagttttg caccaggcgc aaaagaaaaa ggataaggga tcatattttc caataaacca
    16501 atgatcactc cttgtgcaac aagtagcgaa atataaatca ttttttgtaa tttactcatg
    16561 gtgtgtaaag gtctaataaa tcagactgtc ttcgtcacct ccatctgttg aacggacttc
    16621 gatcactaat ttatgtggca aacagacgat ggtttcgcca tttttcgttg cccagcctcg
    16681 acgtacgcag atctggtcat cgcaatctgc ttcttttatc cgtactcgat cgccatctat
    16741 ctcgatcaag ttataatctc cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc
    16801 agccatcaaa gggaatgttt tgatttctgt gccatccaca cgcagtaccg cttctttttc
    16861 tgctgttgcg gtgttttgct gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt
    16921 caaaagcaaa ataataaaaa catcccaagg tttgagcaga cttttttcag caaattcttt
    16981 aaggctcatt tttgttctcc tttttccaga ttaactcacc caattttagc ataaaaacaa
    17041 gaggttgtga cacctgtgtc ttgctttgag taataagaac gaagaataga aaacagcttt
    17101 ccatgttttc taattctttg ggcttattac cgagaagcag caggtggaac accgtttata
    17161 acaagaggtt gtgacagaag tgggcaactt caagaaataa gaaggaattt acgaaaatta
    17221 cttttcaagt ttttgtgaaa tttcggctta tttttcgaag aagctacttc tggaacaccg
    17281 tttataacaa gaggttgtaa cagaggttgt gacacctgtg tcttgctcat ggaggcttat
    17341 ctcgggcatc ttattccttt aattttacat aaaaaggagg ctgtgacggt ttgtctcagc
    17401 ccctttctat tgaaatcttg cattcaaata acgttcttta tctatttatt tcttgccgta
    17461 ctgggaactt ggtctaccgt accaccaatt acctagtgta cgttgtagcc atggaattac
    17521 ccagcgatcc aacccaatcg cacgtccgga accgttcatc aatgcgaaag caacaaaaat
    17581 gaaccaaatg tttacccagt aaaacatgcc tgataagcag aacgtgatca ccaatacaat
    17641 cgttgtcgca ctgcatagcc atgtgaataa tccagcaatc aatgctaatg ccaaggcaat
    17701 ttcaaagaat accatgaatt tttggaagaa cattgctaca tcattgtttg gcatgatcac
    17761 ttgcatgatc tttgtgaacc agtctggtga cttctcaatc acagccattg gttcttcacc
    17821 gtatgcataa ctcaaaccaa aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc
    17881 tgctccgctg gcagctgtgg ttgcatcacc agcagcagaa gcaccactgg caacatctgt
    17941 tacattgtat tcccatgtcc aagggaacac actgtttcca gtgaaccatg atccttcacc
    18001 aaaccaagta cttggtttga acacttcacc attcccaatg atttttttgg aagcttcaac
    18061 gaaccaaact aaaccataga aaatacgaag tggcacactc cataagacat tgccgtaacg
    18121 agaagaatgt cctcgagtca cgttgcgatc atctttgata tggaaaaact catgcattaa
    18181 atattggaac atataataac cagaacgaat atcaaagaag tatttcaaat tcacgatatg
    18241 tttcattacg atagctaaaa atccgcttaa atggatctta tcaaataggt tagctacccc
    18301 ccatttggca ccaatagaaa ccataaatcc ttgatagttt cctttaaatt tatgcttttc
    18361 tccgcctttg atcgaagcca cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc
    18421 ttgaacgatc tgcggtgtcg gtgttcctgg gaattcttca taaaagacta aatcaccaat
    18481 gatatagatg tttttatctt cgtagccttt tgcctgcata tattcattgg cgatcaaccg
    18541 ctgtccgcgt gctgattcta atccaaagtc agcagcgtca gaagttcctt tgacaccagc
    18601 tgtccaaatc agtgtgtgag tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc
    18661 agccacttct acgattggtg cattcaataa taattccacg ttgtgcttac gcaaatagcg
    18721 ttcagctttt gcagcatcat ttcgatccaa catattcaag atcgtcggca ttgcctctac
    18781 gaccatcagt gtgatttcgt ttggatctaa tttataatct ttggctaggc gatttttcca
    18841 gtcgatcagt tccccaatca tttcaatacc ggtaaatcca gaaccacaga caacgaaagt
    18901 cagcatggct ttacgcagtt catcatctgg ttccaatgca gcttttgcta ctgtcttctc
    18961 aatatgttcc cgaatcctta cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt
    19021 gacaccaggt gtaccaaagt cattcggctc tccgcccatt ccaaggatca gctgatcaaa
    19081 aggataggaa cctaatttgg tcttgacgat ttttcgttct ttatcgatac cgattacagt
    19141 gtctgtgaca agttgtacat tcttttgacg agcaaacaaa tgctgcagat cgtattggat
    19201 tgcttctggt tctacacgtc cgccagctac ttcatgcaac tcggtcatca tagtatggta
    19261 cgaatgacga tcgatcagag taattgctac atcttctttt ttcagtttct ttgccaagta
    19321 ttttgtagct gaaacaccgg catatcctgc tcctacaacg acaatatttt gctttgccat
    19381 tgatcatccg ctccttatag actatctata taatgaatat atgataattc ttttagtttg
    19441 atacttatta cacaatctcc atatattata tagatgttct gttcttttgt ctaactcctt
    19501 ttggattttt ttcaaatttt aaaataaagc gttttttcac gaaatttgct gaaagaaaac
    19561 gtttgaaata ttacatgggt tatttatcaa aaatagttga cttataggct aaaaagatta
    19621 gaatgtataa tgaatcacaa agaaaaattg tggactagaa atggaatggt ggggtattaa
    19681 aatgaagaaa aaacgttttg taactggatt tgccgtatta gcattttcag ctctagtgtt
    19741 aggcgcttgc ggcgcagatg acaacggaag ctcaaactct tcttctgaat catctacagc
    19801 acaatcttcg actgcaaaaa gcacaacaga atcatctgca aaagttgtag ctggcggaga
    19861 tttgcaagac ggcacatata aattagaaga aaaaaattat tcaaatggtt accgtgcagt
    19921 ctttgaaatg gttgtaaaag acggcaaaat cactgaatca aaatatgata acgtcaatga
    19981 aaacggcgaa tcaaaaacaa aagatgcaga atacaacaaa aacatggaag caaaatctgg
    20041 cacaaaccca gaaaaataca ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc
    20101 aagcggtgtg gaagtcgtaa ctggagctac tcactcatct gaaagcttcc aaaactacgc
    20161 acaacaattg atccaagctg ctcaagctgg taacacagat acaatcgaaa tcgacaatgg
    20221 tgctgatttg aaagacggca cttacaagct tgaagaaaag aactactcta acggctaccg
    20281 tgtgcaattc gaaatgacag ttgctggcgg caaagtaact gaatcaaact ttgattatat
    20341 cgacaaagac ggtaaatcaa aacaagatga tacagaatat aatgaaaata tgaaagcaaa
    20401 aagcggtaca gaaccaaaaa cttacatccc tactctgaac gacgaattcg tcaaagcaat
    20461 gggcgaagaa gatggctcac ctgctaatgt agaagttgtt acaggtgcta cacacagctc
    20521 acattcattc atcatgtacg cacaacaatt agtgaatgca gctgaaaaag gcgacactca
    20581 aacaatcgaa gtagataata tcgtaacaga aaaataatca cataatcata ctaataaaag
    20641 ttggagggtg ggacgatagc cagattaggc agtcgtctca cctttcaaaa tgaagagagg
    20701 aaaaagatgc ggaaaaaact actattttcc ttaatcacac tgggcatctc ttttatggtg
    20761 ctaactggct gcagcaactc agcaaataaa acaaccactg aaagttcagc agctaccaag
    20821 atattgaaag atccatattc tgatgaacag ttcttgttag ggacttacgt tcgtatccgt
    20881 gtgtatgatg aaggcaaaga atcagcattg aaacctgcct tcgatcgagt aaaagaactt
    20941 ggtgataaaa tcacgatcaa tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct
    21001 gggatcaaac cagtaaaagt ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa
    21061 tatagccaag attctcaagg cggattcgat atggcgattg gtgcaatcac acaattatgg
    21121 cgaatcggtt ttgatgacgc cagaaaacct tcccaagaag aaatcgatca ggcattgaaa
    21181 ttagtggatt atcataagat cgaactaaat gacaaagaaa aaactgttta tctaaaagaa
    21241 aaaggaatga tcatcgattt aggtgcaatc gccaaaggtt acattacgga cgaagtagtg
    21301 aaagtcttga aaaaacaagg agtgaccact gccatcgttg acttgggcgg taatgtctat
    21361 gtcttaggcc acagcccaag aggcgaaaac caagactgga cagtcggcat ccaagatccg
    21421 aacatggctc gcggatctgt tttaggttct attaaagaac ggaacaaaac attagttacc
    21481 tctggaattt acgagcgtta tttagaagta gatggcaaaa aataccatca tttgtttgat
    21541 ccaaaaacgg gttatccgtt cgacaatgat atcgcaagtg tcacgatcat aacagataaa
    21601 tcaattgatg gtgacgggtt gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg
    21661 gaatacgtcg aaagcgaatt gaataacggg acggaagcga tcttcgtgac aaaagacgat
    21721 aaagtatatg tgaccgatcc aatcaaagac accttcaaat tatcggagga ctcccattac
    21781 acgatgggtg accgttctga actaaaataa ggagtacgaa ccatgacact aaaaatcttt
    21841 ttagaagtag ttgaaatacg aacaaaggtg gctagtgtat ttccttttgc ggtaggggta
    21901 ttattctcta tcgctttttt tcatgagata cattggctaa acactttttt attttttatt
    21961 ggaatgctag tttttgattt agcgactacc gctatcaata attacatgga ttatgaaaag
    22021 gctcattctg aagtatataa gtatgaagaa aatgtcattg gccgcagaaa tctatctcct
    22081 gtattgattc gaaacatgat tttagctatg attgcatttg tttttgttat cggcgttatt
    22141 ctaacgatca atacgggttg gcttttactt ttgatgggat ttatctgttg ttttattgga
    22201 gtattctata cattcggacc gattccttta tctcgaatgc ctttagggga aattttcagt
    22261 ggggtgacta tgggccttgg aatttttgcc atggtcgttt atgtcaatac attcggttca
    22321 gatgtctatg atctgacagt caacttttca tctggacaat ttgctttggt aggaaatctt
    22381 tgggctatag cagcaatcgt attagcttcc ttacccttgg tgtttacgat tgccaatatc
    22441 atgttggcta ataatttgcg ggatcttgac cgtgatattg aaaatcatcg ctatacacta
    22501 gttttttata tcggacgtcc tgcgggaaca gtcttattcc agctattgat gtatgcttgc
    22561 tatttcgtga ttttaatggg gttgttgagc ggtatctatg aatggccgat cttggtcacc
    22621 ttcttgacct tgccaattgt gaagaagcat ttggatcaat tcaagcaaac gttaccccag
    22681 cctatcagtt tcagctattc catcaaaaat atggtcttat tcaatgccag ttatgtattg
    22741 gggctattac taacaatcat tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa
    22801 ctgccctgca tcaagtaata agaacagcca aataaaaata gctccttata tttttcgttt
    22861 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttatcagga ggttgtgaaa
    22921 aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
    22981 ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttatcag aggttgtaaa
    23041 atcagtgttt tgtcctgagt attagagaaa caaatggaaa aatagttcct catattttgc
    23101 cgttttttga gctaataccg aaggacagct gattgaacac tatttgttcg gaaaaagtga
    23161 ctaactatga cacaattttt ttcacagtcc cttttttcac ttgatttgat tcgtaccagc
    23221 gaacttgacg agaactttct tggtttggta actatatggt attcttttaa catcctcgtt
    23281 tgcttcttct aatttattta tacaaagtca cactttttta aacatctgtt cttcgataca
    23341 tttctatttc ttttaaacat cgataccaat attttgaact taagagtgca taccaaatgg
    23401 attcatttac tcttcgtcag ttacgaagat aatcaaaatc tccaacttcc ctacttattt
    23461 cgttttgaga attatcgaag caatcttaca taaataacta aaaaacaaaa aaccagaaaa
    23521 tcagtatact gattttctgg ttttacatgg tttgttcaat aaaaagctgt tggaactgtt
    23581 aatattaaat tatcgcatcg ttacgaattc ttccgctcct gttggatgga tggctactgt
    23641 attgtcaaag tcttctttag ttgcacccat cttgatagcc acggcaaaac cttgcagcat
    23701 ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt ctttcttctt ttccgacaca
    23761 gatcaatttc atttcgcatt tttggcgata atcatttaat gcaaagtaca ttggggtaaa
    23821 gcgtgaacga taaactttca attcattttc gccatatttt tccaatgctt cttcttctgt
    23881 caaaccaatt gttgcaattg gtggatgcgt aaagacgact gtcggaacaa gtttatagtc
    23941 aagataagaa tctttctttc cattaaataa acgttctgat aaacgtctac cagctgcgat
    24001 tgcaacagga gtcagatcta atttaccgat cacatcacca acagcaaaaa tatgttcatc
    24061 cgttgtattt tggtatttat ctactttgat aaaccctttt ttgtccaatt ccacatttgt
    24121 attttccaga cctaattttt cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc
    24181 accagttaag gtttcaccat tttcaaatag caccgtatat tcgtcattgt ttttagtaat
    24241 ttctttagca atatgatgtg cgtaagtttt tattccagct tcttggtaca tttggatcaa
    24301 attgtccgaa agcattttat caaacgttct aagtggtcgt tcttttcgat atgcccacat
    24361 gacttcacta cctaaaccac gaaatacacc tgaaagttca gccgcaatgt atccagcacc
    24421 taaaacgatt gctctcttag gtaactctgt taattcaaaa aatccatttg aatcaattgc
    24481 gtattctcca ccaggaatgg tcattttact tgcacggcct ccagtagcga tcaagatttt
    24541 aggagcacga taggtttcac cgtttacttc gatcgtttga ctatcgataa aagtagcgta
    24601 accttttata gcctcgacat gattgctatc taatcctctt tgataagcac cgtgtagaaa
    24661 atcaatatat ttttctcgtc tttctactag accggcgaaa tcaacatttt ttaaatctgc
    24721 ttggataccg taacttgagg catctcgttc aattgtttcc aaaatcgtac ttgcttgcca
    24781 cataactttt ttaggtacac aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc
    24841 aatcagcaaa acttttgcgc catgcatccc tgctctgttt gctgaagcaa taccgccaga
    24901 gccgccacca attacgatgt aatcatatat ttgcattttg ttcctcctta ttgaagagat
    24961 caatcaaaac ctttatttaa cgatcctttt tctacagaaa acaagtgtaa cacttttctt
    25021 gaattttgta tgcgaagatg cttaaaacag ggatatatca gctttttcct tatttacgtt
    25081 ttcggatcat atctccaggt ttcaccggct gtgcgactgg catagttaaa atcatcattg
    25141 gatttggtgc tcggtcaatg gattcgcctt cttcattatg cataacttca acggtttgac
    25201 tgaaatgact gaatcctgga ccataaaatt cgatttcatc tcctacagaa aacaagtttc
    25261 tctgacgtat cgtagcaatc tgtgttgcct catcataaga taatacttca ccgacaaatt
    25321 tatatactgg gattttacga cgttcaccga ataattgttc gttttcagtt ggtgtattgt
    25381 agtaaaaacc tgtagctaat tctcgttgtg ctactttcca taactcgtcg atccattctt
    25441 gcttgcagac atagttttca ggatcttcca tataactgtc tacagccgct ttatagacat
    25501 ttgacaccgt agatacataa tggatcgatt tcattcttcc ttcgatcttg aagctgtcta
    25561 cgccgttttc aatcaattct ggaatatgtt cgatcattga catatccact gcactcatcg
    25621 agaattcttc ttccacttcg cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca
    25681 tatcgaacaa tccatacttc cagcgacaag attgagaaca tccgccacgg tttgcatccc
    25741 gcatcgacat atggttggac aatgtgcatc gaccagaata agagatacac atggcaccat
    25801 ggataaatgc ttcgatttcg gtatccgtat ttttacgaat ctctgccact tcctccattg
    25861 aaacttcacg agctaaaacg acacgttcca acccttcttc tttccagaat tccaatgtct
    25921 caaaattagt tgctgaagct tgagtggata aatggatcgg cagacctggg gcttctgtcg
    25981 cacaaatttc gatcaaagct ggatcagaaa caatcactgc agaaataccg acatcacgca
    26041 aactgcggaa aaattcccct gctccatctt catttccttc atgggtgacc atattagcag
    26101 caacgtatac cttcgcatca tgttcttttg caaaagcaac tccttcagcc atttcttcat
    26161 aagtaaaatt acctgcacgg cttcttaaac catatgcatt cccgccaata tatactgcat
    26221 ctgctccata atggatcgcg gtttttaact tttccagtgt tcctgctggc gctaggattt
    26281 ctggtcgttt caattttctt tttactgtca tttctttcat catactcctt atttgatagc
    26341 agaaggatca atataaaaga atcctgtatc taattcacgg ttttcaggat gcaatttact
    26401 gatttcgcta gtcatttttt ccgctttttc ttgatcccag ctaccttcta aaatttgttt
    26461 ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt tcgcctggag taaataatcc
    26521 gtccaatttc catgtacagt aatgatgctg ataaagttca ttcaattcat tcgagagatt
    26581 cagatcattg ctggcaaaaa tatgtgtgcc atgactatct tcataaatag aataatgtgt
    26641 ctcttctttt ttaggttccg agataaaaag acctctttca cgatcttttt gttcgtcttg
    26701 tttcgtatag ttatagtaat tctgtagcag tggacgtttg gattgatgga tgcatgtagc
    26761 accatacaca agggtttcta caggaatatc gagtttttct tccatcgcct tcatctcttc
    26821 aaaaggaact tcacgagcaa gtactgctcc aactgcgcct tttttcgccc agaaattgat
    26881 ttgacgtgcg ctcgttacta atgtttcagc atcataaata tacggtatac gcaattcagg
    26941 attttttttc atcgtataaa taatacctgt atcaccaact gtgatcacat ctacgtttat
    27001 ttcttttaaa aaagccaaat attcaggtac atttttcatt ttttcaggat gaatgatccc
    27061 atttacagca atattcgctt ttttaccata cttgtgtgct aattctacca attgtcgctg
    27121 ttcttctcgt gtaaatgaag caggcagacg caatccaaat tgctcttcac cgaaatatag
    27181 tgtatctgca cctgcagcaa gcaactgttc tgcttgttca atagtttcta ctgtggttat
    27241 gagttcgatc ataattttct cctcctacaa cttcataata gtagcatagc tctaaaaact
    27301 ttccaatact tcttctattt aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa
    27361 taaagttcga tatattctgt ccaaatataa aaagagattg tgacaaaaat cttgtcacaa
    27421 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
    27481 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
    27541 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
    27601 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
    27661 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
    27721 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
    27781 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
    27841 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
    27901 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
    27961 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
    28021 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
    28081 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
    28141 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
    28201 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
    28261 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
    28321 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
    28381 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
    28441 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
    28501 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
    28561 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
    28621 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
    28681 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
    28741 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
    28801 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
    28861 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
    28921 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
    28981 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
    29041 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
    29101 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
    29161 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
    29221 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
    29281 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
    29341 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
    29401 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
    29461 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
    29521 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
    29581 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
    29641 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
    29701 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
    29761 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
    29821 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
    29881 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
    29941 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
    30001 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
    30061 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
    30121 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
    30181 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
    30241 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
    30301 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
    30361 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
    30421 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
    30481 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
    30541 aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt cgaaccccta
    30601 accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat attcaattta
    30661 aaaaaattat aaatcatttg aa
//
LOCUS       DAZBWR010000019        30202 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000019 DAZBWR010000000
VERSION     DAZBWR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.019"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHGT5_000816"
     CDS             complement(38..1189)
                     /locus_tag="ACHGT5_000816"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN7216823.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHGT5_000817"
     CDS             complement(1202..1639)
                     /locus_tag="ACHGT5_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7216824.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHGT5_000818"
     CDS             complement(1654..3420)
                     /locus_tag="ACHGT5_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN7216825.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHGT5_000819"
     CDS             3684..4361
                     /locus_tag="ACHGT5_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN7216826.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHGT5_000820"
     CDS             4364..5491
                     /locus_tag="ACHGT5_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216827.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHGT5_000821"
     CDS             complement(5691..6131)
                     /locus_tag="ACHGT5_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7216828.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHGT5_000822"
     CDS             complement(6142..8175)
                     /locus_tag="ACHGT5_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN7216829.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHGT5_000823"
     CDS             complement(8177..9880)
                     /locus_tag="ACHGT5_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7216830.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHGT5_000824"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHGT5_000824"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN7216831.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHGT5_000825"
     CDS             complement(10963..11988)
                     /locus_tag="ACHGT5_000825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN7216832.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHGT5_000826"
     CDS             complement(12197..13066)
                     /locus_tag="ACHGT5_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7216833.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHGT5_000827"
     CDS             complement(13525..15417)
                     /locus_tag="ACHGT5_000827"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216834.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHGT5_000828"
     CDS             complement(15414..17141)
                     /locus_tag="ACHGT5_000828"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216835.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHGT5_000829"
     CDS             complement(17157..17807)
                     /locus_tag="ACHGT5_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN7216836.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHGT5_000830"
     CDS             18011..18307
                     /locus_tag="ACHGT5_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN7216837.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHGT5_000831"
     CDS             18479..19099
                     /locus_tag="ACHGT5_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN7216838.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHGT5_000832"
     CDS             complement(19191..20357)
                     /locus_tag="ACHGT5_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN7216839.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHGT5_000833"
     CDS             complement(20369..20722)
                     /locus_tag="ACHGT5_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN7216840.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHGT5_000834"
     CDS             20850..21872
                     /locus_tag="ACHGT5_000834"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN7216841.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHGT5_000835"
     CDS             21891..22052
                     /locus_tag="ACHGT5_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216842.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHGT5_000836"
     CDS             complement(22311..22745)
                     /locus_tag="ACHGT5_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216843.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHGT5_000837"
     CDS             complement(22855..24714)
                     /locus_tag="ACHGT5_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN7216844.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHGT5_000838"
     CDS             24715..24855
                     /locus_tag="ACHGT5_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN7216845.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHGT5_000839"
     CDS             complement(24832..25992)
                     /locus_tag="ACHGT5_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN7216846.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27210)
                     /locus_tag="ACHGT5_000840"
                     /pseudo
     CDS             complement(26299..27210)
                     /locus_tag="ACHGT5_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(27376..28569)
                     /locus_tag="ACHGT5_000841"
     CDS             complement(27376..28569)
                     /locus_tag="ACHGT5_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7216847.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(28653..28853)
                     /locus_tag="ACHGT5_000842"
     CDS             complement(28653..28853)
                     /locus_tag="ACHGT5_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN7216848.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(29277..29510)
                     /locus_tag="ACHGT5_000843"
     CDS             complement(29277..29510)
                     /locus_tag="ACHGT5_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216849.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(29517..29948)
                     /locus_tag="ACHGT5_000844"
     CDS             complement(29517..29948)
                     /locus_tag="ACHGT5_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN7216850.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         8479 a         6440 c         5483 g         9800 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaattata tgggatttta gattatcttt ttagaagttc
    27241 ttgaatgtcg gtatagcccg tgtttccggg cttttccggc atttttgata ttgttagata
    27301 cttacaaatg ttcttattac ctctcaccat ttccccctca aaagtagtaa atggagtagt
    27361 aaaaccggcc ttgtgtcagg ctgccagccg ttccatttct gcccttgcgg aatcgaatgt
    27421 ggcgtgtgca taatagttca gcgtcatggt gatattggaa tgtcccataa tgtactgcaa
    27481 ggctttgggg ttcattcccg cattggctaa ccgggtgcag aatgtatgac gcatagcatg
    27541 gggcgtcgtc gttttcggca acgcttcttc atggctcttg ttgtacttct tcacaagcct
    27601 gccaaacatc ccgccataat ctaccgccgt catgggatag ccgttctgtt tgagaaacag
    27661 gaaattggca tacccgtcaa taatgaacgg ctttgcacct ttgcggtttt ttatcactcg
    27721 ctggaacgcc tgatacactt cctcattcat ggggattttt ctgacgccgc tttgggtctt
    27781 gggttcgtca atatagtaac cgacgccggt atttctcaaa agctggtggc tcacgatgat
    27841 tacccggttt tcaaaatcca aatctttgtc cgtcagtccg cacagttccg aaatgcgaag
    27901 ccctgtcccc agcagaataa cgattgcgtc ataatgcttt tggtaaacct tatcaccttg
    27961 aataaaggac agaaaacttt cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc
    28021 gtcgtccaga acgtcactaa gctggaagtc aaacgggttt tttcggatgc aatcgtcctg
    28081 tatggcggtg taaaaagccg ctttcaggga acgcttgtcg ttattgatgg tcttataaga
    28141 aaggcccttt tctttcatcc gaagcgccca ttcctttgcg tcggacagct tcacactgtc
    28201 aatcgggcag ctccccagcc gatcttgttc cagaatacgc atcagccgtt cccggcccag
    28261 tttggtgttg tgcttcacat tgccccggtg cctgatctgc ttggcgtaga gctggcagac
    28321 ggtcattttc ttgccgatgg tgtctatccc gtcgtcaagg tctttctgga tttctttctc
    28381 cttttcccgg agtgaaatat cctcccgttt acctgccggg gtcttatccg taggcactaa
    28441 cttccatgag taaacaaact gcggttttcc aaaggtatca acatatttgt aagcatatcg
    28501 tccgtctttt ctctggctct ctccagaccg caaaacgcga tttttgctgt ctcgtctttt
    28561 ttctgacatc tcgtcatgct cctttccatg acggaaagag ccgcatatat gcttataaat
    28621 tattataaca tatctgcggc cctttttcac tattagattg tgtccaaagt gtcaattatt
    28681 ttctcaaact gcttgcgttt gatctggatg cggttgccat tcataatgac ccagccagag
    28741 gccggatttt cctcggccag cttacgcaat ttgttttctc cgatacggaa atatttagac
    28801 gcttcctcaa tcgtcagcgt gtatttttcc caaataggca catcatggtt cataatgcag
    28861 ccccctttca caaattcaaa ggtttgtgtg atttaggggc atagacggac ggctgctggc
    28921 acagctccac gggactttca cccccgcgtg gttctcacgc ggccctaccc attgcctgcg
    28981 acgcttttaa cgctcggact gtggctataa gggagtatca tagtcctgcc tgtacgtcgt
    29041 cgcccgcaag ccgccacgct cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta
    29101 gggaaaagta tcatggcgca ccagccgtcc ggctcggcac agacagaatg tgtccgtatg
    29161 ccctatgatg cgccgccgtt atcttgcggg catatttatc aaggtgcgat caggccaccg
    29221 ggaaaacacc gaaacgggga aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag
    29281 ttcgtgacga tagcgtggat gagcttgatt tccagacggc gcttcatggc ctcgtccaga
    29341 cacacatgcg gatagccgtt ttcgtcatag agcgtccggg tacacagctt gttcatgtaa
    29401 ccgctgtaat actgcaaaac gcgctctacg gctccggcat ccccagcgcg ggcggcctcg
    29461 atcaccgaaa ggggcaaaag ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa
    29521 cctcccttcg gcatcaacgc ctttagctgt ttccgcagct ccgataaggt atttgtcctg
    29581 tgccgctgaa cggcactccg ggacattccc acaaggccgc cgatctcacc atctgccata
    29641 tccagaacac agtgcaggat taaaatactc tgctccatgc tgggcagcgc ggcaaaggct
    29701 tcggcaacaa gctcattgtt gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg
    29761 tcgctggggt agtgatctac tgtacagagc ttgtccaaat ctgcctgtga caaagagctt
    29821 agtgacactt cacggtcacg gcggcgcttc atttcagcca agtagctttt ggcctcgtta
    29881 cgcagtaccg tcttgcaaaa agcatcgaac atgcaccgcg cttcatagtc gcgggggata
    29941 gcatccatct tctcaccccc tttctgtggg ggacttaggt ggttctctcc ctttccgcta
    30001 acaagacttt gacaaggccg ccagctaccc gctaaatacc tcctttttga aaatttctca
    30061 aaaaattttt ttggggacag ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc
    30121 aaacctaccg ggcgagaaaa tgctttgcta tgctgctgat ttacatggta tagtacatac
    30181 tcataaccgg aatagccctg tg
//
LOCUS       DAZBWR010000020        29609 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000020 DAZBWR010000000
VERSION     DAZBWR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.020"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..974
                     /locus_tag="ACHGT5_000845"
     CDS             105..974
                     /locus_tag="ACHGT5_000845"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN7216851.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1139..2410
                     /locus_tag="ACHGT5_000846"
     CDS             1139..2410
                     /locus_tag="ACHGT5_000846"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN7216852.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2407..3702
                     /gene="rho"
                     /locus_tag="ACHGT5_000847"
     CDS             2407..3702
                     /gene="rho"
                     /locus_tag="ACHGT5_000847"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN7216853.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3801..4064
                     /locus_tag="ACHGT5_000848"
     CDS             3801..4064
                     /locus_tag="ACHGT5_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN7216854.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4509..4958
                     /locus_tag="ACHGT5_000849"
     CDS             4509..4958
                     /locus_tag="ACHGT5_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7216855.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5015..6433
                     /locus_tag="ACHGT5_000850"
     CDS             5015..6433
                     /locus_tag="ACHGT5_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN7216856.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6433..7293
                     /locus_tag="ACHGT5_000851"
     CDS             6433..7293
                     /locus_tag="ACHGT5_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN7216857.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7467..8240
                     /locus_tag="ACHGT5_000852"
     CDS             7467..8240
                     /locus_tag="ACHGT5_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN7216858.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8387..8752
                     /locus_tag="ACHGT5_000853"
     CDS             8387..8752
                     /locus_tag="ACHGT5_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN7216859.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8900..10108)
                     /locus_tag="ACHGT5_000854"
     CDS             complement(8900..10108)
                     /locus_tag="ACHGT5_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7216860.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10127..10405)
                     /locus_tag="ACHGT5_000855"
     CDS             complement(10127..10405)
                     /locus_tag="ACHGT5_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216861.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10598..11254
                     /locus_tag="ACHGT5_000856"
     CDS             10598..11254
                     /locus_tag="ACHGT5_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN7216862.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11696..12493)
                     /locus_tag="ACHGT5_000857"
     CDS             complement(11696..12493)
                     /locus_tag="ACHGT5_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN7216863.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12490..13674)
                     /locus_tag="ACHGT5_000858"
     CDS             complement(12490..13674)
                     /locus_tag="ACHGT5_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN7216864.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13699..14946)
                     /locus_tag="ACHGT5_000859"
     CDS             complement(13699..14946)
                     /locus_tag="ACHGT5_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN7216865.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15161..16906
                     /gene="dnaX"
                     /locus_tag="ACHGT5_000860"
     CDS             15161..16906
                     /gene="dnaX"
                     /locus_tag="ACHGT5_000860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN7216866.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16926..17240
                     /locus_tag="ACHGT5_000861"
     CDS             16926..17240
                     /locus_tag="ACHGT5_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN7216867.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17265..17861
                     /gene="recR"
                     /locus_tag="ACHGT5_000862"
     CDS             17265..17861
                     /gene="recR"
                     /locus_tag="ACHGT5_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN7216868.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17925..18569
                     /gene="tmk"
                     /locus_tag="ACHGT5_000863"
     CDS             17925..18569
                     /gene="tmk"
                     /locus_tag="ACHGT5_000863"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN7216869.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18584..18913
                     /locus_tag="ACHGT5_000864"
     CDS             18584..18913
                     /locus_tag="ACHGT5_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN7216870.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18927..19865
                     /gene="holB"
                     /locus_tag="ACHGT5_000865"
     CDS             18927..19865
                     /gene="holB"
                     /locus_tag="ACHGT5_000865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN7216871.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19901..20725
                     /locus_tag="ACHGT5_000866"
     CDS             19901..20725
                     /locus_tag="ACHGT5_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN7216872.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20718..21065
                     /locus_tag="ACHGT5_000867"
     CDS             20718..21065
                     /locus_tag="ACHGT5_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN7216873.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21134..22006
                     /gene="rsmI"
                     /locus_tag="ACHGT5_000868"
     CDS             21134..22006
                     /gene="rsmI"
                     /locus_tag="ACHGT5_000868"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN7216874.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22115..23236
                     /gene="dinB"
                     /locus_tag="ACHGT5_000869"
     CDS             22115..23236
                     /gene="dinB"
                     /locus_tag="ACHGT5_000869"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN7216875.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23290..23892)
                     /gene="nrdG"
                     /locus_tag="ACHGT5_000870"
     CDS             complement(23290..23892)
                     /gene="nrdG"
                     /locus_tag="ACHGT5_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN7216876.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24207..26396)
                     /gene="nrdD"
                     /locus_tag="ACHGT5_000871"
     CDS             complement(24207..26396)
                     /gene="nrdD"
                     /locus_tag="ACHGT5_000871"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN7216877.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26722..27489
                     /locus_tag="ACHGT5_000872"
     CDS             26722..27489
                     /locus_tag="ACHGT5_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216878.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27479..29521
                     /locus_tag="ACHGT5_000873"
     CDS             27479..29521
                     /locus_tag="ACHGT5_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216879.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9494 a         5320 c         6199 g         8596 t
ORIGIN      
        1 attgatttta gtagaaaact accgaaatgt ttggtaaaat agggtcgttg gttaagaatt
       61 gtcttaacaa atttagtttt acaaaactta ggaggaattt tattatgcca gtagtatcag
      121 gagctgaatt tttaaaagct gctcgtaaag gcggttacgc tgtcggcgga ttcaacacaa
      181 acaacttgga atggactcaa gcaatccttg aagcggctga agcaaaacaa gcaccagtac
      241 ttatccaaac atcaatgggt gcagctaaat acatgggtgg ttacaaaatc tgtaaagatt
      301 taatcactga tttagttgaa gcaatgaaca tcactgttcc agttgctatc cacttagacc
      361 atggtgatta cgaagctgct ttggaatgta tcgaattagg ctatacttca gtaatgttcg
      421 atggttcaca tctaccattt gaagaaaact tgaaattagc taaagacgtt gttgaaaaag
      481 cacatgcaaa aggtatctca gttgaatgtg aagttggttc aatcggcgga gaagaagacg
      541 gaatcatcgg taacggcgaa ttagctgata ttgaagaatg caaacaaatg gttgctacag
      601 gtatcgacta cttagcttgc ggtatcggta acatccatgg tcaatatcca gaaaactgga
      661 aaggattagc ttttgaccac ttacaagcaa ttgctgatgc tgtaggtcca gacatgcctt
      721 tagtattaca tggcggatca ggtattcctc aagatcaaat cgaaaaagct atctcaatgg
      781 gtatctcaaa agtaaatgtt aacactgaat tccaattatc atttgctgca gctactcgta
      841 aatatatcga agctggtaaa gataaagaag gtaaaggatt cgaccctcgt aaattattag
      901 ctccaggtaa agcagcaatc gttgaagatg ctaaatctca tatcgactgg ttcggttcag
      961 ctaacaaagc gtaagcatcc acataacaga tacgatatat aggaatggag aacgactttt
     1021 gaagtcgctc tccattcttt tttacttaaa acggcagacc atgctcgttt ctttttttta
     1081 tctatgataa aatacaagca tatccatgaa aaaagattaa taaacaaggt ggaaacagat
     1141 gaaaaaaatc gtaatcaaag gaaaccgacc tttagctggt gaagtaacga tcagcggtgc
     1201 aaaaaatagt gcagtcgcat tgattccagc tgcaatctta gctgattccc cagtgatctt
     1261 ggaaggtgtg ccggacatac aagatgttca ttcattaatt gaaattttag agatcatggg
     1321 cgcaactgtc catttttcca acaacatact agaaatcgat cctcgagaag tcgtatcgat
     1381 tccgatgccg cacggaaaaa tcaatagttt gcgtgcttct tattatttta tgggaacact
     1441 gcttggtaaa tttggcgaag cggtagtagg cctgccaggt ggctgttatt taggccctag
     1501 accgattgac ctacacgtca aaggatttga agctctaggt gctgaagtga caaatgaaca
     1561 tggtgccatg tatttacgga cagaaaataa aggattgcgt ggcaatcgga ttttcatgga
     1621 cgtggtttca gtcggagcaa cgatcaatgt gatgttagct gctgtaaaag caaaaggaca
     1681 gacagtaatt gaaaacgctg cacgagaacc ggaaatcata gatgtggcaa ctttactgaa
     1741 taatatgggg gcaaaagttc gaggagcggg tacagatgtg atccgtatcg aaggagtcga
     1801 aacattacat ggctgtcggc atttcatgat tcctgaccgc atcgaagcag gaacctatct
     1861 agcattagca gctgcagtag gaaatggtat caaagtgaaa aatgtgatct ttgaacattt
     1921 ggaaagcttt atagcaaaat tgcaggaaat tggtgtccat atgaaaatca gcgaagatga
     1981 aatcgaagtt tacccctcga aagaattaaa acccgcaaac atcatgacct atccttatcc
     2041 aggatttgcc acagatctgc agcagccact tactgcgtta ctattgatga ctacaggcac
     2101 ttcagaaatc atcgatacga tctatgcgaa acgtgtgaac catgtgccgg aactagcacg
     2161 aatgggcgct gatattaccg tggaaggaaa tatgatcatc gtgaatggtc caaataaact
     2221 tcacggcaca gaagtcgtgg cttctgatct tcgggcaggt gcttgtttag tgattgctgg
     2281 actattggca gaagggacaa cgacgattta caatgtagat tatatcttac gcggttatga
     2341 tcatattatc gagaaactaa cagccttagg ggcatcaatt gaaatgatag aggaagtggg
     2401 agctgaatga gcgattattt gacaatggca gaactagaga acaaaacgtt aaaagaagtt
     2461 tatagttatg ccaaagaatt taaaattcct tattacagta agatgaataa aaaggaatta
     2521 tctctagctg tgattcgtgc acaagcagag aaacaaggat tttattatat ggaaggaata
     2581 ttggatatcg ttggtcaaga tggttacggt tttttacggc caatcaatta tggaccaagt
     2641 gcagaagata tctatatttc tgcctcacaa atcagacgat tcagtttgag aaatggggac
     2701 aaagtggcag gaaaagcccg tccacctaaa gaatcggaac gttattatgg tttgatgcac
     2761 gtggaaagcg ttaatggaaa agatccggaa gaagcaaaag aacgtccgca ttttccagcc
     2821 ttaacacctc tttatccgca aagacagctt caactggaaa caacaccgca aagactatct
     2881 acccgaatga tcgatatctt ttctccaatc ggttttggtc agcgtggatt gatcgtcgca
     2941 ccgccaaaag cagggaaaac ttctgtatta aaagaaatcg caaatggtat caccgaaaac
     3001 tatccggaag tagaattaat cgttctactg attgatgaac gaccggaaga agtaacggat
     3061 ctagaacgaa gcgtgaaagg cgacgtggta tcttctacat ttgatctgca gccgcaaaac
     3121 catactcgag tttctgaatt agtattggaa cgcgcaatgc gtttagtcga agataaacga
     3181 gatgtcgtta tcttgatgga tagtattact agacttgctc gggcctataa cttagtcgtt
     3241 ccaccaagcg gacgtacatt gagtggcggg attgacccag ccgcattgta taaacctaag
     3301 aaatttttcg gggcagcaag aaacatcgaa gaaggtggca gtttgacgat tttagcgacc
     3361 gctttagtcg atacaggaag ccgtatggat gacgtgatct atgaagaatt caaaggaact
     3421 ggaaatctag aattacaact ttcacgtgaa ttatctgaac gtcggatctt cccagccatc
     3481 gatatcaaaa aatcaggtac ccgaaaagaa gaattattga tgccaagtga agaattagaa
     3541 gaaatttgga aacttcgaaa aaatatgact ggagattcat tagaatatac ggaacaactg
     3601 atccgctttt tgcgtaaaac aaaaaacaat cagcaatttt tcaaagaatt tcaagatgtc
     3661 tcctttggta aaaaaaatcc gaccagaaat ctgaaaagat aattgcaacc ttgactgaaa
     3721 catgttaaga tgttaatgtt gtaaatcgac aaatcaactc tggttcagca aatggagcag
     3781 ggcaaaggag cgaaaaaatc atgaaagaaa acatccatcc agattaccac ccagtagtat
     3841 tcatggattc aacaactggt ttcaaattct tgtcaggttc aacaaaaact tcacaagaaa
     3901 cagttgaatg ggaagacggt aatacatacc cagtcatccg tgtcgaagta acttctgact
     3961 cacatccatt ctacactgga cgtcaaaaat tcacacaagc agacggacgt gtggaccgtt
     4021 tcaacaaaaa atacggtctc aaagacgcaa acgctgcgga ataataaaaa cgctgataac
     4081 tcaacgttta gcagactatc tgatagggat agtctgtttt tttatgtttt ttgccaattt
     4141 aaaacttcgt ttgatgctga aaaagtcgta ggatcataat gcacaaaaca atgaaaaact
     4201 agtatatagt tttaattatc cacttgataa aaacacctta tattaacgaa atgaagaggg
     4261 gttatttcga agtaaaaaac catttaccta aaattagaag ctaagctttc ctttcaagga
     4321 aggctttttg ttttgttata gacttgcgat tgtttgtgag ctagatcgtt cattttaatg
     4381 acttatacac gcggatgatg ggtctttttc ctctgttcat tcataatatt gaacggtaca
     4441 gctagcgatg ctatactgac ctcagttcca agaaaacgtt aacatttttt gtgggggaga
     4501 ggttttttat gaggtttgat caattgatcg cagaagaact gattgttttc gatccatcga
     4561 tagattcaaa aggatcatta tttgagcgaa taggggatgt gttgaaaaaa acaaataagg
     4621 taagaaatgt tcaaaaagtc gttaaggcac tattcaaacg agaggaggag atctccacag
     4681 gaatagaaga tggatttggg attccccatg caaaaagcaa atatgtgaca aaaccagcac
     4741 tgtgtttttt tcatacagcc aaattaacgg actatagcgg gatagatgaa atgccaattg
     4801 aatatatttt tgccatcgta gttcctgaaa aatcagcaga tatgcatcta gagattctca
     4861 gcacactctc cagacgattg atgaatgcag attttagaaa gggaataaaa gaagccacta
     4921 cgccagcaga tatcatcaga cagattagtc aagaatgaaa atagtagagt ctttgtccga
     4981 gtatcgatag tgaagaaaaa ggaggaaaat aatcatgaaa atcgttggtg taactgcttg
     5041 cccgactgga attgctcata cttatatgtc ggcagaaaaa ttagaagtaa cagcaagaaa
     5101 tctaaaccat caagcaaaat tcgaaacaca aggtgtaaaa acagaaaatc agttatctga
     5161 acaagaaatc aaagaggcag atgcaatcat tcttgcagta gataaagaaa tcgaattgga
     5221 ccgatttgcc gggaaaaaag taaagcgtgt ctctacaagc cgagcaatca aagaaccgca
     5281 agtagtaata gacgaagcat tgagagatat tggcgtattc gttgtgtcta atgaaaaaga
     5341 accagctgca aatgagaaaa agccgacaat ctataatcat ttcatgaatg gtgtgaatta
     5401 catgcttcca tttgtgatcg caggcggaat catcatcgca ctgagttttg cctttgggat
     5461 tacagcggct gatcccaact cagcagacta taatgtgtta gcagctgcat tttcaaggat
     5521 cggaggagat acagcgtttg ccatgatggt tcctgcgtta ggcgcgggga ttgctacatc
     5581 gattgctgga aaagcaggtt ttgccccagg aattgttgcc ggattactgg cctctacagg
     5641 tggttcaggt tttcttggcg gaatgattgg tggcgtgcta gcagggtata tctgtgattt
     5701 tctggcaaat aaaattagcg taaaaaaaga attttctgct atttatcagc tgattgtcgt
     5761 ccccttttta tcgatcttaa ttattgggct tttgatggta tttgtgattg aacaaccgat
     5821 cacatgggtg ttggatgctt tgacaaattg gctgaatagc ttgggaaata catccgggct
     5881 attgttcgga ttacttatcg ggatcatgat ggcagctgat atgggtgggc cgatcaataa
     5941 atcgacttcg actttctcga tcggtttgat gtcagctgga gtaaatgcac cgattgctgc
     6001 ttgtatggct gcaggaatga cacctcccct aggtattgct ttagccactt tattattcaa
     6061 acataaattt accaaagaag aacggacttc aggtcaatca tgctggattt taggcgcttc
     6121 gtatattaca gaaggtgcta taccgtttgc ggtatctgac ccgatccgcg tgatacctag
     6181 tttgatgaca ggttcagcag tcgctgcagg gatttccatg gcagtaggag ttacttcaat
     6241 ggcacctcat ggagggattt gggtgatgct aattccaaat gttatgaaca atttacctat
     6301 gtatgtcttg gcaattgcat caggtacagt cactacagcc tttatgcttg gactattgaa
     6361 aaaaacagtg aaagagaaaa aacaagacaa gtggacgaaa aatgaaaatc taacaaatga
     6421 ggtggaagcg taatgttata tacaatgaaa gagttattga aagtggctaa agagaatcaa
     6481 tttgcagtac cagccttcaa tatttgcagc tatgatatga tgaaagcaat catcgaagaa
     6541 gcagagagat tagaagcacc agtaatctta gaaattcatc ctgatgaaat cgcctattta
     6601 agtgatgagt ttgttgcggc agtaagagcc tatgcttata caagtaaact gccgatcgtt
     6661 atccacttgg atcatggcgg tacgatagaa gatgtcttac gggcgattcg aaacggctat
     6721 tcttccgtga tgatcgatgc gtctttgaaa tcattcgacg acaatgtaca gattacgaaa
     6781 aaagtggttg cgttagctca tgcggtaaat gtctcagtgg aggctgagct tggaacgatt
     6841 gggaataatg gttctgctga aggaggtgct gaaacaatca tttatacaga tcctgatcaa
     6901 gcgaagattt ttgtgaaaga aacaggaatc gatacacttg ctgtagcaat tggaacagcg
     6961 catggcttgt atccgaaaaa cagaactcca aaattaaata tcgacttact gaaagaattg
     7021 aaccaacaaa tcgatcttcc atttgtttta catggcggtt caggtaatcc ggacgcagaa
     7081 gtcagcgaag cagtaaaata cggagttgcc aaagtgaatt tgagctcaga tcttaaaagc
     7141 gtctttttca atgctttgag agaaatattg agtgaaaatc catcgatgta cgagccgaat
     7201 gcaatttatc cagcagctaa taaaaaggta caagaagtgg tcaaacataa aatggaaatt
     7261 ttgaacacag taggcaaggc tagtttatac taaaaataaa aatgtgtggt caagagggta
     7321 tgacaatagt cttgtcatac cctcttaacc acacacgatg ttcagaagta gttttttcga
     7381 aaaatttctt cttatttctc gaagccaatc acttctgtca caacctcttt tttcctgtta
     7441 ctatataaag taaatggggt gattttatgt tgaatgaagt ggagaataga cagcaggaga
     7501 ttgtgaatta tctaaaaaac aaccaatttg caagagtcac cgatttgatc gagttagtaa
     7561 attatagcga agccacgatt aaacgtgacc ttgtttttct ggaaaaaagt ggattgatcc
     7621 gtagagttcg cggtggcgcc atgcttattg acaatcgaaa aattgacgtg ccttacctga
     7681 tgaagatcac caaattagaa gaagaaacag aaaaacgtaa gattgcagat acagcttctt
     7741 ctttgattcg agatgatatg gtcttattct tggattccag tacaacctct ttacacttag
     7801 tcagaaacct agcaagattt gatggtttgc agatcattac gaatggcatc gtcacagctg
     7861 ctatgttgag tgaatataca acggcaaaag tatctgtatt gggaggggca attgttccga
     7921 aacgagcaac aatcaacggt tcaaaagcct ataacgatgc attgtcatat tatgcggatt
     7981 tagcgataat gagttgtcgc gggctagatt tcagccaagg agtaacagaa actcatgaag
     8041 gagaggcaat ggtcaaaaaa gcttttagaa aacaatcaga ccggcttgcc gttttagtta
     8101 cacagcaaaa aattgcacat aaattcatgc atcaagccat tgcttttcat gaaatcgatt
     8161 atctggtgac aagtaaagaa ctgacaaaag aacagtatgc gaaagtaaag gaacaccata
     8221 tcgaatgtct gtacggataa attattacgt gaatgttcta agtggcttcc tcatttttag
     8281 ataggacctt atctttgctc gcttctctca gccgatggta taataatgaa aaggttatct
     8341 tgttagataa tcttactttt ttatccagga gaaaggagtg cagaaaatga aacatgtgaa
     8401 ggcgttggta gtgaaggcaa tcatgatctg ggcgattctg tgggtcgtgc tgacaggttt
     8461 atacggtgtt agctttatgg attcaacgat tgttggggta attattgtag tcatgatcta
     8521 tgtgctaggg gatctgttga tcttacgtaa agtagggaac atcgcagcga cgatcgcaga
     8581 tgctggttca gctgcagtta tcttgtggct ctatctatat tttatgaatg atacagtcga
     8641 tatttggatg aaagtattga ttcctgctct attgatcggt gtggcagaat ggttcttcca
     8701 caagtggttg ctaagtcaag gcatcgtacc agatgaacgg aaaatggaat aaatcaacag
     8761 ttaaactatt cttatcgata aatgaaagct ttaaaaataa atagatgttt aattaagaat
     8821 aatcataaac tatttttact caaaaaaccg ctcagattat tttcagatct gaacggtttt
     8881 ttttatctat tcatcaagat caaatttcat ttttcaatga ttcaatcaat tcgccctctg
     8941 cttcatgact atgttttcgg ccattaacaa gatagagtgc tgtacatatc aatggaatga
     9001 ctccggcaat cacatacaat cctctaaatg aagtaagaga ttttagcatc cccatcaaat
     9061 aaggaccaac gcctaagcct aggtctaaac caataaagta ggtagataaa gcaatactga
     9121 ttcgatgttc gcggacgatt tttaaacaaa cagcctgtcc atttgacata aacgtaccat
     9181 aacctaggcc gactaatgct ccagagataa gcaatgtcac actattcgtc gttacactca
     9241 gtaaaaataa tcctgctgtt aagaataaat agctaggata catcacgtat tgttctcctt
     9301 tgacatcgaa aatccttcca gtcaatggac gtgtaaatgt gatgaccaaa gcatatacga
     9361 cgaagaaaaa tgacccagca gcaactaagt ggattacttt tgtataagaa gataaaaatg
     9421 ataaaacact tgaataagaa agacccatca aaaaggcaat tcctgtaatg aaaagtgctt
     9481 tcttttcaat aaaactattg atattccatg agcgaagaga tattttttgt tcttcagata
     9541 atggaatatt ttttacagga aagacaaggc aggcaatcgt aacaaagaag accagtacaa
     9601 ttgaaaacca gataatgaaa cgaaaatcag tcatattcag caaaatcatt cctaaaaatg
     9661 gcccaacagc ggcagctaaa ctagtactta atccataata attgatgcct tccccttttt
     9721 tagatgcagg gatatatgca gtaacgatag catttgtagc tgtagaaact gtaccgtagc
     9781 cgaatccatt caagaatcga actaaataca atgccccgat agttggcgta taaagatatg
     9841 ctaatgttga aaggagatag aagatcgcgc cgcctcttaa ggtaaacttc cgaccaaata
     9901 gttctaactg tttccccatg aataagcgtg ccagcaatgt tccgatgata tagatcccag
     9961 aagcaaaacc tgcttggctc aagctggcgt gtaaggtgtc ttgcgcaatg actgccatga
    10021 tgaccatcaa cagataataa atcaggtaga caataaaatt gatcaatgtg attccaataa
    10081 aatatttatt aaataatttc ttttccatgt atgttataca ttccccttaa gctgaatatg
    10141 cttttaattt ttgtatttga acttctacat cgtgataatt ggatggatag cgatcaacaa
    10201 gttggcgaat aaataaatct cttgtagaca taaacagatt taaaaattct tcgcattcgt
    10261 ttgttggatt ctcatccact gctttgatca attcgatcga gttttgatac gcatcattta
    10321 aaaagactaa agaagctttc atatcaaatc ttgtttgata tgctgtttct aatcgattaa
    10381 tgattgtgtt catccagtaa ttcatgtttt ttccctctat tcttaaaaaa ttttacttct
    10441 gcagtataac gaataaaaaa aaggaaaaaa atcgcataag tgatggaaaa aggtacttaa
    10501 caaatgttaa tatcattagt aaaatacctt tggactttaa aaagttttaa agtactgtat
    10561 gatcttttta gatgaaagtt tggatggggg agcatcattg gatagtagag tcttaaagga
    10621 atatttggat aaaaatgatt ttccgattat acgcaaagga tatcataaat atttgacatt
    10681 cgaagggatg gaagattccc atacgtatat cttgaaagag ggaacggtaa aagcaagtgt
    10741 tatttcaaat gatgggagag aatttaacct tcgctatatc aaagatcttg aaatcgtctc
    10801 tcttttaaaa gacgaatatt cacaattcat cgattcgccg tataatattc gagttgaatc
    10861 agaatatgca gagttttatc gaatcaatcg tgtgcgcttt tggcaaaata tcaaccaaga
    10921 cccaaagcta caacactatg taaaagagta ttaccgctat cgtctgcttt attcgatgaa
    10981 aaaaatgcag caaatgctat taaacggaaa gctgggagct gtttgtacgc aactttacga
    11041 attatatgat ttattcggtg tgcggacaga agaagggatg ctgatcgatt tttcgatcac
    11101 gaatgaagaa atcgccaaat tttgtggcat ctcaactgct agcagtgtaa acagaatttt
    11161 aaaacaactg aaaaaagatg gtattatcac cgttaaagac catcatatcg ttattttgaa
    11221 tatggacttg ttagaagaca atattatctt ttgatgaaca aagaggttgt gaaatcagcg
    11281 ttttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt tgccgttttt
    11341 tgagctaata ccgagggtca gctgattgaa caccgtttac caagaggttg tgagcctgcc
    11401 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    11461 tgtgacttgt gaccgaggag ttggtaggag aacaccgttt accaagaggt tgtgaaaaag
    11521 ctgttctgca tcaagtaata agaagagcca agtaaaaaca gtctcttatg tttttaactt
    11581 ggctggactt atcaccgtag atgtaagctt ttgaacgccg tttatttgca acaaaagggg
    11641 ctgtgacacg atttttgtca cagccctttt tttgcgaaca ttcatttagc aatttttaga
    11701 aacttctgcc gccaccgcca aaagtcccgc caccgctaga atgggtagta cttccgccac
    11761 cgccgcttcc aggaggtggg cttttgggga ttcttcttgt cgtgacaaaa gaatttgtca
    11821 gacgatcggt tttttcagtt aatttgatac tggatttttc tcggaatgga tatttgtaga
    11881 tgcctgattt caattgataa cgcgaaacag tcacaaaata aaagacaaga ctaaggataa
    11941 gagccaaaac aacagcaatg atgatttcta ctgtcgtcaa aaccttatag cgtgtaattt
    12001 tgccagtttc ttcgtctaca cgataatgtc ctcctggaac tccgtcttcc acatatgttg
    12061 ctgatttaga caggtaagct tttgcagcat tgtaataggc agcatttgtc atctgatcat
    12121 aaacatcatc taagatcgaa tcgattctgc tatcagtcaa ataatcgatc atattaccag
    12181 aagtcgaaat gtagatttca cgctgtccca tatctaatag caatactgag ccattttgat
    12241 catttccgac tgcgtctctt aagtaattat cagcaaagtc tcttggttct tctgaattag
    12301 atgtagtcgt tacgatcaag actcgtcctt tgattttttc attcagcttt tctgcctgtt
    12361 gttccaaaga ttggatttga tctgttgtga ataacccagc ctcatcattt accgtagctg
    12421 tatctgcatg agtagccacc ggccagccaa cacttactag caaaagaaag aataaaaaaa
    12481 attttttcat catatcaagt aacctccgat cagaaagagg atcgctagcc cagcgaaaat
    12541 cccaagagcg gtcagtccca attttttctg gctgatcggc aggacaccgc tgacctttcc
    12601 tgtttgtcca ttcattgcgt agtagtagac ttttttactt gagtcattgc ttcgataagt
    12661 aacaagccat accggcagta atacgtaatt atttacttcg tttgtgattg aagcagaagt
    12721 tcgcacaccg gtcaatgttg tatatccatt tgcggtatct tttaacagct tctctgaata
    12781 gtcttttaat tcagattgaa tctgtgattt gattgcctca tattcgatat ctcttttttc
    12841 tgcttggaac ccggctaaat actggctttt aaaatcaatt gctttatcta acgggaaagg
    12901 ttgcacgcct tcgaccatct tttgttgcgt attttttgat aaggcatttt ttactagttc
    12961 tttaaaagaa agcttgcctg ctcttgaaac agcaaattgc ttcgtttctg tgtattcgat
    13021 gtcacctatc cgccatatgc ggataactgt accattcgcc tgcatggcac catctacttc
    13081 agcatctgta gcccaataag ggaaatacac acctgtcaac ttatcgattt gatccttatt
    13141 gaaaaaagct ttgggaacaa accatttttt cttggtccaa gcgagaaatc tgttgattgc
    13201 ttcttctttc tcgacagcaa aaggaagtac tttatttggc aagaattctc cgcttaaacg
    13261 tcccgatagt acgacgggat tatggcagta gtaacaataa gtcgcagcag ttgtcgcatc
    13321 agtcacgatc tctgcaccac agttgggaca aagaaaaaga tctatcgctg cggtattatc
    13381 tgcagattcg tccgctgtat ctgaaaaacc gcctgcctcg tcaaccgctt tgcgctcggc
    13441 ttcattcata tcggcaagct gttcttgggc tgtaaaagta aaatcttcgg taggtgaagg
    13501 ttcttctttg ctttcttcgc cagccatacg cgcttctttt tgcttttgct catattggct
    13561 gacttcttct tctgtataga tattcaggca gtattcgcaa tgaaattttt ggtctttggg
    13621 atcaaaagtc aacggaccgc cgcaattagg acatttatgt gtaagaacat ccatcagctg
    13681 cacacctttc taaataaatt aattaacggg gattccttta aacggtttac cacagtttgg
    13741 acagaattta gggatgccgt tggaaagatc cacgatctca ttgcattcac tgcatcgcat
    13801 ttgtagctta ggctgaacgt tttctacagg tttggcagct ccgcagttgg aacagaattt
    13861 tccggaattt tctgtcccgc aaactggaca tgtccaagta tccgcatttc cttgtgcagt
    13921 ttgttgattt tgtttttctg cttgttgttt gatttgttct tggttatttt gagctgcttg
    13981 agaaagataa gaaccatttg cattcattcc catacctaca cccataaagc cggtcattgc
    14041 tcctgcttca ttttttccgg ctgcttccat accacgtgca atggaacctt gcacgtagcc
    14101 ttcacgtacg ctaggatcac ctagcatcgc cccttcgtta cgcatattga tcagtttcac
    14161 agaatcatcg gtgtaagaaa tgctggctac agccacagaa acaatctcca tcccacgtaa
    14221 ttcacgccaa gaatcatcca atgcagtatc catgtatttg cttaattcca aactcttaga
    14281 tggtacataa gaaatccgtt gaccatctgc agacatctga ttgattgctg attgtagcgc
    14341 tgttaggaat tcagctaaat actgctcatt gatatcattg atttccactt gtgttttgtt
    14401 tttagggatt gcgtttgtat aaaataaaat cgggtcaacg acatgaatag aataagtgcc
    14461 atgtgcccgt aagaataatt ctgcgttata gaaattgtca aagtagttca acggcgcgct
    14521 tgtaccgaat ttgattccct ttatttcttg gagattgata taaaaaactt gttgtttctg
    14581 cggtgtgacg ccgccaaatt tgaatcggtc aaaagtttca gcaattgctt ctttcagtgt
    14641 tccattgaac atggaaggag ctgcgtcatt tttaatggtg taataacctt cctcagcggt
    14701 ataatcgatg atttttccac catcgaccaa aagcatcatc atgtttggat agacatggac
    14761 gactgttccg tctgttaaaa catcttctgt tcctttacga ttggagccac gtttgtcatc
    14821 tttgcggact ttgacacctt ttgtcatgac cgttgtatca ctcatattgt ctggttcgat
    14881 gacttctagc cattgatctg ccagaccgcc gccaaccata cttgttgctg ctttgataag
    14941 acccataaaa aattcctcct ctgattcagc tagtgccaaa caattattgt ttatttatta
    15001 taccatagga agatgtgaaa cttctcggac tagagacgga attgaaatgg aaaattgcgt
    15061 attgtttaaa tacctgaaaa agccgagaag gatagtagtt tttttaccta atatcacgta
    15121 taataagaga tagtgaattt tttaaaaaag ggggatcctt atggcatatc aagcacttta
    15181 tcgagtctgg cgttcgcagc gtttcgaaga catcgttgga caaaaagctg tgacacagac
    15241 actaaaaaat gctattgttt cccacaaaac ctcacatgcc tacttgttta caggacccag
    15301 aggaaccgga aagacgagtg cagcaaagat tttcgcaaaa gcaatcaact gtccgaacag
    15361 taaagacggt gagccatgca acgaatgcga aatgtgtcga tcaattactg ctggtacgca
    15421 agaagatgtg atcgaaatcg atgctgccag caataacgga gtcgaagaaa tccgatttat
    15481 ccgtgatcgg gcaaattatg cgcctacaaa ggcagcgtac aaagtgtaca ttattgatga
    15541 ggtacatatg ctttcaacag gagctttcaa tgccttgttg aaaacattgg aagagccaaa
    15601 agaaagtgtc atattcatct tagcaacgac cgaacctcac aaaataccag ctacgatcat
    15661 ttcacggact cagcgatttg attttaaacg gatcaatacg gcagatattg tcgaacatct
    15721 tgaatttatc ttgaaagaat cgactatcag ctatgaacca gctgcattga atgtgattgc
    15781 ccgttctgca gaaggcggga tgcgggatgc attgagtatt cttgatcagg caatctcgtt
    15841 tagtgaagga acaattactt tagatgatgc catgcaagtg acaggtagct taactgacga
    15901 aatgatggac agttacttga gtgcttgcgt gaaaaaagaa gtaccagaag cattggaaac
    15961 attgaaccaa attctcgcag ccggcaaaga atcacgtcgc tttctagagg atatgttaca
    16021 atactgccgt gacttgctga tgtatcagca agcacctaac ctagttgagg aacaatcgag
    16081 tacgatcaga agtacattta cagaattggc acaaactgtt tcagcggatc agctgttttc
    16141 aatgataaag attttgagcg atactcaaaa tgaaattcgt tttaccaata gtgccgctat
    16201 ttatttggaa gtcgctacag tcaaacttgc acagctaccg tctgcttctg ttccttcagc
    16261 agatgtagca tttgcagatg atcagatagt aaaacagcta caacaagaga ttaaggaact
    16321 aagaaacgaa atcaaggaac tacgcagtaa tacaggaatt gagaatacaa aagtacagcc
    16381 ggtacagcaa acaaatcata aaaaacagac aagtacctat cgtgtaccga cggaacgagt
    16441 atataaagtt ctgcgggaag cgacaagaca gcatctgaac caagtaaaag aagtatggga
    16501 agacttgctg atgagtctat ctgtcactca gcgagcaatg cttaaagcaa gcgaaccagt
    16561 tgcagctgga cctacaggac tagtgattgc gtttgattat gagattgttt gtcaaagagc
    16621 agcaaatgac gaagaattgc agctttcggt acacaacaac ttaagccgaa tgatcaaaga
    16681 ttatgcgcct gatcctgttt atattacaag agaaagctgg ccagaacttc gaagaaatta
    16741 tttgacgcag gcacaagaag agggaacaga aaactctttt gcttctgaag actcagaagg
    16801 agaaatcgag ttgcttcctc aagaacaaga agtaccagaa gaaacacttg tagtaaaagc
    16861 agaggaactg tttggctccg aagctgttac agtggtagat gattaataaa aaggagtaga
    16921 taattatgat gcgtggaatg ggcaatatgc aaggcatgat gaaacaagta caaaaaatgc
    16981 aaaaagaaat ggcagaagca caagagctgc taaataaaac agagtttaca ggtaaagcca
    17041 ccaatgaatt ggtcaaagcg gtttttacag gcgatcgtcg catgaaagat atccaaatca
    17101 atgaagaagt cgttgatcct gatgatatcg agatgcttca ggatcttgtc gtaatggcag
    17161 tcaatgatgc gatcgcacaa attgatgtgg aaactgagaa aaaattaggg aaatacgcaa
    17221 aaggtttgcc aggattttag ttttaaactg aaggagtaat gaatatgcaa tatccagaac
    17281 caattgcgaa actgatcgac agctacatga aacttccagg aatcggtcag aaaacagcga
    17341 caagactcgc cttttatacg atcgatatga aagatgaagt agtaaatgaa tttgctaaat
    17401 ctttgttaag tgtaaaacgc gatttgcatt tttgtagtat ctgcggaaac atcacagaag
    17461 aagatccctg cgaaatatgt agaaatcctt cacgtgatcg aagcgttatc ctagtggtcg
    17521 aagagccgaa agatgtaatg gcaatggaaa aaatgcgaga atatcacgga ctttatcatg
    17581 ttctccatgg tgtgttatca ccgatggaag ggacaggtcc agaagacatc aatatttctt
    17641 ctctgttgca gcggctgcat gatgcagatg taaaagaagt gattatcgca acaaatgcaa
    17701 caacagaggg agaagcaaca gctatgtatc tctcacgtct gatcaaaccc gcaggtatca
    17761 aagtgactcg tctagcacat ggtctgtctg taggctctga tatcgagtat gcagatgaag
    17821 ttactttatt gaaagctgtc gaaggaagac gtgagttata aatttctaat taagtgtttt
    17881 ttcagataca atataaaagc agcacaagag atgggggcaa caatgtgaac ggactattta
    17941 ttacaatcga ggggcctgat ggtgcaggaa aaaccagcat cttgaatgaa ctttaccctc
    18001 ttttgaaaga agtagcaaaa acagaaatca ttcaaacaag agaaccagga ggcattccga
    18061 ttgctgaaga gatccgagca gtgatccttg atccaaaaaa tgatcggatg gatgaacgta
    18121 cggaagcact gctttatgca gcagcaagaa gacaacatct agtcgaaaag gttctgcctg
    18181 ctctggcaaa agggaaaatc gttttatgcg atagatttgt cgacagttct cttgcttatc
    18241 aaggtgcagg ccgacgcatc ggcgttaccg agattgctcg gttgaatgag tttgctacag
    18301 aaggtactac gccggatttt accctttatc ttgacgtaga ttcagacacc ggattaagaa
    18361 gaatcaagaa aaacagacaa aatcaaatcg accgtttaga ttcggaaggt ttggaattcc
    18421 accagcgtgt acgtcatgct tacttaaagc tggcagaaga gaatccggaa agaatccaca
    18481 aagttgatgc aagaaaaagc tttgaagaag tgctgcaaac aagttatcat acaattattg
    18541 aacagtatcc gcaatttttt gaaaactagg aaggtgaacg tttatgaaaa ttattttagc
    18601 gattatccaa gacaaagaca gcaaccgttt atcaaatgaa ttgattgatg caaatatccg
    18661 tgccacaaaa ctatcatcga caggcggctt ccttaaggct ggaaacagta cgtttatcgt
    18721 gggtattgaa gatgaccgtg tagaagaagc attggaaatc atcaaaaaaa cgtgtgaatc
    18781 aagaaagcaa tttgtatcta cacctgttac actggatatt tcgatggatg ggggcgtccc
    18841 ttatccagta gaagtagaag taggcggtgc cacagtcttc gtcttacctg ttgaaggatt
    18901 ccatcaattt taggaggagc tgagctatga atcaagaaat aaacttggct caaatgcaac
    18961 cgatcgtgta tcagcagctt caacggagtt ttgagcatgg gcgtatcgct catgcttatc
    19021 tttttgaagg ggagaaagga acaggaaaac atgaagtagg tatatggctt gcacagcacc
    19081 tgttctgtac ccaaatgaaa gacaatctgc cttgtggaaa atgcaacaat tgtcagcgga
    19141 tcgaaaagaa agaacatcca gatgttttgg ttattgaacc ggaaggacaa accatcaaag
    19201 tcgatcagat cagacgtttg cagacggaat tcagtcgcag cggttacgaa tcaagaaaaa
    19261 aagttttttt gatcaaagaa gctgaaaaaa tgaatagcag tgcggcaaac agtttgttga
    19321 aattcttgga agagccccct ggagattttt tagcggtgtt agaaacagat gcagtaggaa
    19381 gaattctccc tactattcaa tctcgttgtc aagttctgca ttttcaagaa ctttcgaaag
    19441 aagcactgat ccatcgattg caagaagagc agattccatt ggaaaaagca aaacttttgg
    19501 ctttcttaac aaacagttta ggaaaagcag ttgaaatctc tcaagatgaa tggtttaatg
    19561 atgctaagga cttgatttat caatggttcg tttacttgca aaagcaagat actcaagcct
    19621 ttatctatgt ccaaaaaaaa ttggtaaaat catttaaaga aaaaacacaa cagtttacag
    19681 gattatctgt cttgatgttt tattatcagg aagcacagcg tcaagcagta gcaaacggcg
    19741 aatatggcaa agtgaaacag ataaaccaga taattgaacg gatattgatc gcagagcaga
    19801 aactacgcag caatgtcagt tttcaggctg ttgcggaaca gttcgttttg catacgattt
    19861 tttagcttgc cgcaaaggat gatgatcaag gaggatggaa atggtagaag tagtaggtgt
    19921 ccgatataga gatgcaggtc atatctatta ttttgcacca ggagactctg aatatgttta
    19981 tagtgacaag gtattagtcg aatctcagca atccttacat attgctactg ttgtccttcc
    20041 taaaaaagag attgatccca atgaccttcc tgaagctatc cgtccgatct tgcataaagc
    20101 gtcacaagct gaattagaaa aagacaaaaa aaacaaaaaa gatgcttatg atgcattgaa
    20161 aatcgcaaaa gaaaaagtgc agttgcgtga gttggaaatg aaaatggtca aagccgaata
    20221 cacatttgat cgaaataaat tgattttttc tttcacagcg gacgggcgaa ttgactttag
    20281 agaattggta aaagatctgg ccgctgtatt tcgtactcgt attgagcttc gacaaattgg
    20341 tgtccgcgat gaagcaaaga taataggcgg aattggtcca tgtgggcgtc cattatgctg
    20401 ttcttctttt ttaggcgatt tcgtgcctgt atcaatcaaa atggcaaaag atcaaagttt
    20461 atctttgaat ccaacaaaaa tttccggttt atgcggacgc ttgatgtgct gtctgcaata
    20521 tgagagcgaa gaatacgaga cagcgaaaaa agaactccct gactttggaa aagtaattgc
    20581 tacacctgat ggtaaaggga aagtcgtagg gttgaatctg cttagccgta tcgttagtat
    20641 tcggctgact ggacgggaaa atcctgtcga atatgaatgg gatgaattga aagaatttat
    20701 ccaagtaggt gagatgaatg gataagagat cattatacga cggattgaat caattagaat
    20761 cagagttgag aaatacatta acagaacttg ttgaaatgaa gcatgctttg catgagatcg
    20821 tagaaaaaaa tacaactttg gagatggaaa atcaacgtct gcgggaacac ctgcgggaat
    20881 tgaaccaagt tgcacaagct ccagctgaaa atgtgaagca ggaactttct aagtctcgta
    20941 tgaatcttga aaaaatttat gaagaaggat tccatgtctg ctacgattta tatggatcaa
    21001 gaagggacaa tgacgagcct tgtgcgtttt gtctagaagt gatctatcgc gaaagcggtc
    21061 gatgaccaat aacaagagga gactggagcg gagaaatact gctccagtct ttttattaag
    21121 gagattgtga cagatgtata atcaaaaaag tttcaaggga aatccggtat atggggattt
    21181 atatcttgta ccgacgccaa ttggaaattt agaagatatg agctacagaa gtgttcgtat
    21241 gctgcaagaa tccgatctta tcgcaagtga ggatacgcgt aatacacaaa agttattgaa
    21301 tcattttgaa atccatacac cccaaaaaag tttacatgaa cataattata aagaacgtgt
    21361 ccctcaattg attgctttgt taaaagaagg aaaaacgatt gctcaagtca gtgacgcagg
    21421 aatgccttcc atcagcgatc ctggacatga gctagtttta gcctgcataa aagaagggat
    21481 ttctgtgatc tctatcccag gacctacagc tgggatgacg gctttgattg cttcagggct
    21541 agtgcctcaa ccatttttat tttatggctt ccttccacgt aagaaaaaag aacagaaaga
    21601 aacattgaaa aagctaaaag aacagacagt tacgctgatt ttttatgagt caccttatcg
    21661 gatcactgct acattgacga atttcttaga aatttttgga aatcgccaag tcgttttgtg
    21721 tcgggaattg acaaaggtcc atgaagaata ccttagagga agtacggaag agctactcgc
    21781 ttatatcgaa gaacatccag taaagggtga atgttgtctg cttgtagaag ggaatttgtt
    21841 cgaaatgcca gtgacggaag ttgatgaaga gttgggaacg ttaaaggaac aagtagaagc
    21901 aaagatcgct tcaggcgaaa aaccaaatgc tgctatcaaa gcagtagcgc tgaaaaatgg
    21961 gctgaaaaaa caagaagtct accgtcagta ccatgaatta gactgagata gtataagttg
    22021 tctataatga aagactaatg aggaaatcag ctactaactt ttcaaatctc ttgaacgcga
    22081 acgaatgttc ttgtataatg aataagaggt gaggatgatg gagctgcaat ttcctttaca
    22141 taatgataca tcaagaaaaa tcattcatat tgatatggat gcttttttcg cttcggttga
    22201 agaacgagat catcctgagt ttcgcggaca tccattagta attgccagac accccagtga
    22261 cactggtggg cgtggagtag tgactacagc aaattatgaa gcaagaaaat tcggaattca
    22321 ttcagcaatg agtgcacaaa aagcgtatga gctttgtccg caagccatct ttaagcctgg
    22381 cgaccatcat aaatacagcg atatttccaa acaagtacgg gaagtattct atcgttatac
    22441 agatattgtg gaaccagtta gtatcgatga agcgtatctt gatgtgaccg aaaataaaat
    22501 caattgcaag tcagccatca aaatcgcccg aatgatccaa gcagatattt gggagagtgt
    22561 ccagctaaca tgttctgcgg gagtaagtta caataagttt ttggccaaat tagcatcaga
    22621 ctatcaaaaa cctagaggaa tcacggtagt tgcccctcaa gacgctgtgc catttttaaa
    22681 agctttgcca attgaaaaat ttcatggggt agggaagaaa actgtaccta gaatgcatga
    22741 gctagggatt tatacaggaa aagacctata tgaatgcaca gaaatgatgt tgattcgaaa
    22801 ttttggaaaa atgggctatt ctctttatcg taaagtcaga ggaatccatg attctccagt
    22861 aaacgttaca agagagcgaa aatctgtggg aaaagagcat acttatgggc agccgttgca
    22921 aacggaagaa gcagtgctga ctcagttgcg ccaactagct gaaaaagtag aagaagcact
    22981 gagaagagtc caaaaacatg ggaaaacagt agttttgaag gtcaggtaca cggactattc
    23041 tactgtgacc aaaagagtga cattgccaga atatatctat aaaaaagaag cactctttta
    23101 tcaagcaagc cttatatggg aggaaatctt gggagtagaa aaaggaatcc gattgttagg
    23161 gattactttg acgaatctgg acccgatgac ttatgaaaac atcgtccttc cattatggga
    23221 aaatcaagaa atataaaaac agctttgaga gttgaaaccg gaaatccgtt ttctctccca
    23281 aagctgtttt tatatcaagt cttgcttttt gatttgttca taggtttctg ttgcatccgt
    23341 acatttttcc caaatcacaa cttgattgga ttctaaagat ttttggacgt caatgatccg
    23401 ctggttgcta cttccgcgaa attgtagttt taggtcacgt ttgcttaatt caaagcgtcc
    23461 gtcaacaagg atatcgattt gcgaaaggag ttctaattta tcttctgaat cttgtagcaa
    23521 ttcatcaaag gtatagcctg tccatgacca gatatctttt gtcgtaccga actcttttcg
    23581 gatacgtcgt acgacttgca gacaaacatc tgtattcaaa aaaggttcgc caccaagtaa
    23641 agttaatcct tgcacgtaat cgttgcgcaa gtcagcgatg atttgttctt ctaattcagg
    23701 tgtgaatggt ttgccatatc gaaaattttg tgcagctttg ttgaagcagc cttcgcaggc
    23761 aaataagcag ccactaacgt aaagactgtt gcggacacct tcaccatcga caaaattaaa
    23821 cggtttgtag tcagcaatat agttttggct gtagtctttt gctaaccatt cttttggttt
    23881 tggatttctc ataaaaaaac ttctttcatt taaggttcaa agatatagct attgaagata
    23941 aaatctactc gaccacataa ataagaacaa actgacctaa atccattgtt caagaaagct
    24001 tgttcttatt ttgatagagg gcgttcaaag atacaaagcc atcgaaagag ggtggtgaaa
    24061 aaagacttgt catagcctct tattctgaat aaacggtgtt caaaagcttg catctgcagt
    24121 aataagccca actaaacgaa aaatatgagg aactattttt gtttagctat tcttattact
    24181 tgatgcagga cagctttttc acaacctcat ttcatgtgtt tcacacgtga ggaaatttct
    24241 ttatggcggc catgtaccat cggacgagct tgcggatttc ctaaatagcc acaagtacgt
    24301 ttgactacat cacatgtttt tggatcatga tttccacatt ctggacattc gaaccctcgt
    24361 tctgtcggtt tgaaatcacc ttcgaaacca cattgatagc aatgatcgat tggcgtattc
    24421 gtaccaagat acccgatttt gtcatatgca aaatcccata cagcttctaa agctttcgga
    24481 ttttgttgga gcatagggta ttcacagtag tggatgaatc cacctgaaca atattttgga
    24541 taatccttct caaattccaa tttttcaaat ggcgttgggt ttttacgcac atcatagtgg
    24601 aaactgtttg tataatattc tttatctgtg atattttcga tgatgccgaa tttttctgta
    24661 tccaaacggc agaaacggtc agttagactt tcacttggag ttgaatagac actgaaatga
    24721 taaccatatt catcccccca tgcatctgca ttagctttta attctttgac gatatcaagt
    24781 gtgaaagctt ttgcttctgg gtttgtttcc cattcgccac cgaagaaagc tgaagcaact
    24841 tcatacaatc caatgtagcc taacgataca gtagctcgac ggtttttgaa taattcattg
    24901 acagaatcgg tacgtgaaag acgtttcccg aaagcaccat acatatataa aattggcgca
    24961 tttgctggaa tggcttcttt acaacgttct acacgataga gaagcgcatc tttcatgatt
    25021 tctaagcggt cagacagtaa ctgccagaat ttattctgat ctccttttga ttcgatcgcg
    25081 attcttggca aattcaatgt aacaacacct aaattcattc gtccgacatt tacttcttga
    25141 ccattttcgt ctttccaccc ttgcaagaat gaacgacagc ccataggtac tttgaaactt
    25201 ccagttaatt caacgatttt atcgtaattc agaatatcag gatacattct tttcgttgca
    25261 cattccaatg ctagttgttt gatgtcgtaa ttaggatctt cagggtttaa gttcacacct
    25321 tttttcaaag tgaagatcag tttagggaag atagctgtcc gcttttcact ccctaatcca
    25381 ttgattcgaa tctgtaaaat tgctttttga atttctcttt caaaccagtt ttctcctagt
    25441 ccaaatccta atgaagtgaa aggagtttgt ccatttgaag taaataaggt attgatttca
    25501 tattccaaac tttgcatagc atcgaagata tcttttttgg tttttgcttt tgcatattct
    25561 ttttggcgtt ctggcgaatc gatccattct tccgcgtctt ttagatgttt tttatagttc
    25621 aattctgcaa aaggagcaag caattcatct gttcggtcgg cagaacagcc accatattga
    25681 cttgacgcta cattggcaat aatctgtgag atttgtgctg tggcggtctg gatagatttt
    25741 ggcgattcga cttcggcatt gccgattttg aacccattgt tcagcatgcc tttgaaatca
    25801 atcaagcagc aattcgtcat cggtgtataa ggatgataat ccaaatcatg gtaatggata
    25861 tcgccttttt gatgcgcatt tgctatatgt ggtggcaaca ttttcagtcc gattgatttt
    25921 ccaacgatcc cggctgtcag atctcgctgt gtattgaaaa cattactgtc tttatttgcg
    25981 ttttcgttca cgacagtttg gtccttgttg attagctttt caatcgaaaa gttgatatct
    26041 gttgctttac tgcgagcaaa atctcgtttg gtacgataat tgatgtactc ttctgctaat
    26101 tcatattcgt ttttttctag taatatatgt tccacgatgt tttgaatctc atagattttg
    26161 acattttcag caaaccggct tttgatttct tggacaacca agtctgcaat catttgaatt
    26221 ttttcataag taagtggtga taatgagccg tgaattttct tttctgcttt gatcagtgct
    26281 tcattgatct tttgttcatc gaaatcaaca tggcggccat cgcgtttgat gactttcatc
    26341 tgggatgtct ctggctgctg agtaacgaca gttttttctt cataatttac tttcattatc
    26401 ttcaactcct gctaaattgt tcttgtctat cataaaacaa aatcggggaa ggagcaacta
    26461 aatatcggct atatatagta ttcaatgtgc tgtataattg gatttctata ctatatgtag
    26521 tgtatatttt attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata
    26581 tttaattttg ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga
    26641 caaaaacata agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa
    26701 aatgaaagag gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc
    26761 tacggcagca aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa
    26821 ggcgagtttg ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc
    26881 ttgtccacga tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca
    26941 aaaatgaaaa acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa
    27001 gagttcaatt tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt
    27061 gaacggatga ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat
    27121 atagaagaac tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt
    27181 gcagctgcta gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc
    27241 tctttggact cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa
    27301 gaaaaagcaa cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga
    27361 attttattta ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga
    27421 gagttctttg agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat
    27481 gctgtataaa ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt
    27541 tttcagtttg actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca
    27601 atctttagtc agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg
    27661 atttatcatt actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa
    27721 tagaaggcaa aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc
    27781 aggtatatat gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt
    27841 actcggtatt attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga
    27901 tcttactagt ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt
    27961 catcatcctt ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat
    28021 tcgaagtttt ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca
    28081 ccgattactc gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca
    28141 ttttagggaa atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt
    28201 ttttcctttt ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac
    28261 atttcggtta tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt
    28321 tttgataatg ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat
    28381 tacagtcatt ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat
    28441 tttatcctac caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga
    28501 gaaaaaaatc aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt
    28561 gcattataaa gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac
    28621 tgctaatctg atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc
    28681 gaacatttct ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt
    28741 tcggaatatt tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat
    28801 tcaacaaatg cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact
    28861 gattgtagat gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt
    28921 agatgtatca ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat
    28981 aaagtggctc aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga
    29041 aattagcaca aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag
    29101 ctctcgtttg aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg
    29161 tatcaatatt tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat
    29221 tttgacagtc cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac
    29281 taagctgggg attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac
    29341 cttctttcca cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac
    29401 tcagtatata aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct
    29461 tatctatctt ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg
    29521 atatacggaa taagacaaga taataaatag aactaaattt tgaataccat ttatttggaa
    29581 taagaggttg tgacacgatt tttgtcaca
//
LOCUS       DAZBWR010000021        29291 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000021 DAZBWR010000000
VERSION     DAZBWR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.021"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..689)
                     /locus_tag="ACHGT5_000874"
     CDS             complement(237..689)
                     /locus_tag="ACHGT5_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN7216880.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(702..1112)
                     /locus_tag="ACHGT5_000875"
     CDS             complement(702..1112)
                     /locus_tag="ACHGT5_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7216881.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1387..1599
                     /locus_tag="ACHGT5_000876"
     CDS             1387..1599
                     /locus_tag="ACHGT5_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFN7216882.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1602..1814
                     /locus_tag="ACHGT5_000877"
     CDS             1602..1814
                     /locus_tag="ACHGT5_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216883.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1954..2109
                     /locus_tag="ACHGT5_000878"
     CDS             1954..2109
                     /locus_tag="ACHGT5_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216884.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2106..2330)
                     /locus_tag="ACHGT5_000879"
     CDS             complement(2106..2330)
                     /locus_tag="ACHGT5_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN7216885.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2408..2596
                     /locus_tag="ACHGT5_000880"
     CDS             2408..2596
                     /locus_tag="ACHGT5_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216886.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2735..2863
                     /locus_tag="ACHGT5_000881"
     CDS             2735..2863
                     /locus_tag="ACHGT5_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216887.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2922..3257
                     /locus_tag="ACHGT5_000882"
     CDS             2922..3257
                     /locus_tag="ACHGT5_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216888.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3490..4431
                     /locus_tag="ACHGT5_000883"
     CDS             3490..4431
                     /locus_tag="ACHGT5_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFN7216889.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4433..5323
                     /locus_tag="ACHGT5_000884"
     CDS             4433..5323
                     /locus_tag="ACHGT5_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN7216890.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5366..6166
                     /locus_tag="ACHGT5_000885"
     CDS             5366..6166
                     /locus_tag="ACHGT5_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN7216891.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6181..7032
                     /locus_tag="ACHGT5_000886"
     CDS             6181..7032
                     /locus_tag="ACHGT5_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7216892.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7233..7694
                     /locus_tag="ACHGT5_000887"
     CDS             7233..7694
                     /locus_tag="ACHGT5_000887"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7216893.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7720..8172
                     /locus_tag="ACHGT5_000888"
     CDS             7720..8172
                     /locus_tag="ACHGT5_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216894.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8278..8472
                     /locus_tag="ACHGT5_000889"
     CDS             8278..8472
                     /locus_tag="ACHGT5_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216895.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8503..8796
                     /locus_tag="ACHGT5_000890"
     CDS             8503..8796
                     /locus_tag="ACHGT5_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216896.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8727..9266
                     /locus_tag="ACHGT5_000891"
     CDS             8727..9266
                     /locus_tag="ACHGT5_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN7216897.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9341..9808
                     /locus_tag="ACHGT5_000892"
     CDS             9341..9808
                     /locus_tag="ACHGT5_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFN7216898.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10527..10694
                     /locus_tag="ACHGT5_000893"
     CDS             10527..10694
                     /locus_tag="ACHGT5_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216899.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            10720..11064
                     /locus_tag="ACHGT5_000894"
     CDS             10720..11064
                     /locus_tag="ACHGT5_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN7216900.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            11069..11350
                     /locus_tag="ACHGT5_000895"
     CDS             11069..11350
                     /locus_tag="ACHGT5_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216901.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            11453..11767
                     /locus_tag="ACHGT5_000896"
     CDS             11453..11767
                     /locus_tag="ACHGT5_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN7216902.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            11745..13439
                     /locus_tag="ACHGT5_000897"
     CDS             11745..13439
                     /locus_tag="ACHGT5_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN7216903.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            13459..14637
                     /locus_tag="ACHGT5_000898"
     CDS             13459..14637
                     /locus_tag="ACHGT5_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN7216904.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            14600..15286
                     /locus_tag="ACHGT5_000899"
     CDS             14600..15286
                     /locus_tag="ACHGT5_000899"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN7216905.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            15286..16446
                     /locus_tag="ACHGT5_000900"
     CDS             15286..16446
                     /locus_tag="ACHGT5_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN7216906.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            16456..17331
                     /locus_tag="ACHGT5_000901"
     CDS             16456..17331
                     /locus_tag="ACHGT5_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216907.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            17328..17639
                     /locus_tag="ACHGT5_000902"
     CDS             17328..17639
                     /locus_tag="ACHGT5_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216908.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            17629..17982
                     /locus_tag="ACHGT5_000903"
     CDS             17629..17982
                     /locus_tag="ACHGT5_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN7216909.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            17972..18373
                     /locus_tag="ACHGT5_000904"
     CDS             17972..18373
                     /locus_tag="ACHGT5_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216910.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            18366..18770
                     /locus_tag="ACHGT5_000905"
     CDS             18366..18770
                     /locus_tag="ACHGT5_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216911.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            18782..19387
                     /locus_tag="ACHGT5_000906"
     CDS             18782..19387
                     /locus_tag="ACHGT5_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN7216912.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            19407..19769
                     /locus_tag="ACHGT5_000907"
                     /pseudo
     CDS             19407..19769
                     /locus_tag="ACHGT5_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19772..19954
                     /locus_tag="ACHGT5_000908"
     CDS             19772..19954
                     /locus_tag="ACHGT5_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216913.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            19972..23391
                     /locus_tag="ACHGT5_000909"
     CDS             19972..23391
                     /locus_tag="ACHGT5_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN7216914.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            23442..24179
                     /locus_tag="ACHGT5_000910"
     CDS             23442..24179
                     /locus_tag="ACHGT5_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN7216915.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            24189..26480
                     /locus_tag="ACHGT5_000911"
     CDS             24189..26480
                     /locus_tag="ACHGT5_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN7216916.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            26504..28585
                     /locus_tag="ACHGT5_000912"
     CDS             26504..28585
                     /locus_tag="ACHGT5_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216917.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            28602..28751
                     /locus_tag="ACHGT5_000913"
     CDS             28602..28751
                     /locus_tag="ACHGT5_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216918.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            28748..29194
                     /locus_tag="ACHGT5_000914"
     CDS             28748..29194
                     /locus_tag="ACHGT5_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216919.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            29196..>29291
                     /locus_tag="ACHGT5_000915"
     CDS             29196..>29291
                     /locus_tag="ACHGT5_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN7216920.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT        10393 a         4446 c         6114 g         8338 t
ORIGIN      
        1 aatataaatc tttctctatc atgtaattta tttagttttt catcattttt ttgttctaat
       61 tcataagctt ctttttttat tgtgttagct ttcgacagtt tagtgattcc tataattaat
      121 aaaataagtc caatcaataa taacattcaa aactcctcat ttctttgata taattattgt
      181 agagtttact cagaaatgag gaaagagtcc atgtgaaagc atgggctttt ttatttttaa
      241 tgttgtaata ctaaatgttc aagattatga ggaagcccta aatattcaat aatttggaac
      301 tgactcatat cagataaatc ttgttcatct attgttctag ttaacatttc aattgcaaac
      361 atattagctt cagcttctat accataaatc atacaactaa ggttgtttgc tctaaaagct
      421 gctgtgcaaa tacctttatg gagtctaacg tgggctattt catgccataa tacaaatctt
      481 ttcatagaag gtgataggtt ttgattaatt aatatcacat aacatctttt attgtgtatc
      541 gtttgtccta atgttttatt ccctaaatca gtttcaatta aatctatatc agaatatttg
      601 attattttat atggattaat cgcatcagat ccaaactcta caaataattt ttctatttct
      661 tctaatatcc agtatttgtt taaatgcata ccaaagccca cctattgacg atatttttta
      721 ggagtgaatt ttttctttgc tttaactttg gttaaggcaa gtgcaagttg aatggcatcg
      781 tataattttt ccttatcttc atcgtctaaa ggttcaccat aataatttgt ttcagcatct
      841 gaattcaagc cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt atctttttct
      901 gttaaatcat aataatggcg tttatcagta cgtcctaaaa gataatcagt tgatacatta
      961 aagtaatctg caactttttg tattttatct ccagatggag tacttttatt ccatctagac
     1021 atgcttccat ttccgaaatt taactttctt tcaagttctg ccaacgtcat tgaacgttct
     1081 tttaataaga tttttatacg gtctaatagc ataggatatg cattcctttc aaaataccga
     1141 caaaaaaagt atttttctgc aaattatcta tttacaaata gagaatttgc agttatacta
     1201 gttttgtaag caagatattt acggcataca aagactaaaa gcaataaact aatagtctcc
     1261 ccagacatca gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca taatagcata
     1321 tttgcagttt ttgtcaaact tattagacaa aaatattaga aaatattcga taagggggta
     1381 tgaagcatga ctttaaaaca gcaagtatta attgctttag taaagaaagg ttggagtcaa
     1441 agagagttag ctcgacgaat gggtatatca attacttatt tacgagatat cttcatcgaa
     1501 aaaagaaaac ctaaggaacg tttaaaacaa atcgaagaac ttttggatat caagttagaa
     1561 gtagattcta atgatcagcc atcagaacag gaggcgtaaa aatggtacaa cgtttgagca
     1621 aagcggagtt taaagagaaa tataagtatt ctgaaagtac ttatcaaaga agaatgaagg
     1681 aatttaaaaa atcacgtttt agtgaggggt ataacgcagt tacttccaaa gaaataacaa
     1741 ttgatgtcga tttatatgaa cagtttagaa catggcgttc taaaaatcga tttagagtta
     1801 gagctgtttg ttaatagcta aaaaatctaa attcaccact tctgaaaaga ggagttgctg
     1861 ctctcctttc aagtttgcca aagtccacgt tttttcattt tggcaactcc tcttcttgga
     1921 ggtggtgacg gcgagagacg aaggagggat gacatgaagg aagaatcaaa aatagggatt
     1981 ttgaatgaga acaattacac aagattaatt attgaaacag aagaagggaa aaaagtagca
     2041 gaagttacat tgaccgatgc aactcctgct gacggttatc gtattcgatt aactcctaaa
     2101 tatgattaac cttttggtgg gtgtggatcg tttccgtgac tatcacggct ttgaattttt
     2161 ccatctctgc catgaataaa tagctcagat ccttgatttt tagaaatctc tctagcgatt
     2221 tttactgctt ctgattttgt attagtatgt gcagtcgctt tagaatttcc agcacctttg
     2281 acattccaac caccattgga attaggtaca acatgttgat tttttcccat agttttcacc
     2341 tccatttcta tagaaatatt ttatcaaata gaaatggaga aattcaataa acgaaaggag
     2401 aagtgatatg gcatatacct tgcaacaaga acatcaaatt ctcggtttga ttaaacaacg
     2461 caggaaacaa ttacaagatg accgtgcagc gcttagaaaa gccgatgagc tatcagatag
     2521 gcaagctgaa ctaattgctt ctgaacttga ggatttgaga atgctagaaa taaaaaatag
     2581 ggagattaga ttatgaagaa gacagacaca ctttttatag gattcatttt ggggttatta
     2641 gtgattgtag cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt gatggtttta
     2701 atcaatctgc ttgattcaaa agaaaggagc aactatggca cgagaagaag cgctaaaaat
     2761 cggtaaagtg attgctgata atcggtgggg aaatagccgc cctattattt taagcaagca
     2821 acatatcgaa aagcaaaaag catggcaaca aataaaaaag tgactctgcc agccagcaaa
     2881 gagtcacata caaatttata aaactaggag aattatatca catgaataag aaaattgaaa
     2941 atcttatcga agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag
     3001 cccaaaaaga aggggaactt aaaagtctcg tttatggtga gacaacagaa attttacttt
     3061 gtcttgctat gcaagaagaa catttagatg aaaatctccc attgtcagca catattatgc
     3121 gtagaattgc tgtggatgct tacgaacaag cgaaaaatga agaagaaaat caaccttcaa
     3181 atcatacttt tgttattaat aacaaggaag atttagctga tgtgatgact cgtattctca
     3241 agggggaatt tcaatgatga atgaaaaaac aattgatcta ctcattattt tatttttcac
     3301 gttagtgatt gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct
     3361 tgtgctttgc gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg
     3421 acaactattt aactgatcct gattatgcta aaccaccata tgaagcacca attgatgagg
     3481 aggatgaaga tgagtaaatc taccttagaa atgagccatc aagaatggct tgaagaccgt
     3541 aagaaaggca tcggaggttc ggatgttgga acaattttag gattgaataa atggaaatct
     3601 ccttatcaac tatggctaga aaaaacagga caagtagtac ttgaagaatc aggaagtgag
     3661 cccgcttatt ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca
     3721 ggcaaaaaag tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga
     3781 gcaaatattg atcgggaagt agtgggagaa aatgccattc tggaatgcaa aacagccaat
     3841 caatttctcg gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt
     3901 caacattaca tgaacgttct aaacaaagac tattgttaca tcgctgtctt aatcggtggt
     3961 caaaaattta tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa
     4021 caattagtag agttttggga aacaaacgtt cttggaggta tcgagccaat tattgatggt
     4081 agtcaagcga cagctgattt cttgaaagag aagtatgcag atgttgaaga cgttcaaacg
     4141 acgttaccaa ttcatttcga tgaactagtt gaacagaaaa acgaactcaa acggactaag
     4201 aaagaaattg aatcagctat ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa
     4261 cgtgaagcca gtatcggtat cactcaaaaa aacatcatca gttggaaatt tgtccgtacg
     4321 agacgtatga actcgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag
     4381 atttataacg ttactgaatc aagaaggcta accgaaaggg agatcaaata atatggcaac
     4441 aaatgaatcg ttaaaaaatc aattggcagc aaagccacag aaacaagttg caccaggtca
     4501 attaggactt aaagctttga tgaatacacc aacaatgaga aagaagttcg aagaggtgct
     4561 tcatgacaat gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta
     4621 tcttgcagat agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa
     4681 tcttgggcta gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa
     4741 gcaaacagga aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca
     4801 attagcccaa cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga
     4861 actaaaaagc tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc
     4921 tgatgaagtc attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt
     4981 ctattggacc aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa
     5041 gacaaagtta agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt
     5101 gagaaatctt ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc
     5161 ggatgagaga gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga
     5221 agaagatatt cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca
     5281 aactggatta tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc
     5341 ttgcttttct agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt
     5401 gatgaattca ttcgtttgga ccaatcctta tatgtataaa ttatggaact tgtgtttgat
     5461 gaaagcgagt catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag
     5521 cggagaattc gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg
     5581 tgaacatcaa gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg
     5641 aatgttgaac atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt
     5701 gtaccaaagc agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca
     5761 cacaaacaag aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca
     5821 accacgcaaa aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt
     5881 attcaaacag atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga
     5941 tgacattcgg aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat
     6001 tgaatggagt cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg
     6061 agaaaaatac gacacaatgg cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa
     6121 aacgcttgaa tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc
     6181 atggaaactg ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc
     6241 gaatgtcctg aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca
     6301 gaacgttgta agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac
     6361 ttacaaacag aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa
     6421 ggtggttctg ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt
     6481 gtcgatcaag aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg
     6541 ttaggaaatc caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta
     6601 gcgatggcag cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt
     6661 atcgggttac aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga
     6721 aaaacgattg agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat
     6781 gatatcggtt cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg
     6841 ctaaattcgt tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt
     6901 ggtgaagaac tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct
     6961 gatggattta cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga
     7021 gcgatcgcat gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa
     7081 ttataggtga tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga
     7141 taagtaagct aattatttat cgggatacag aaagatttct cactgtttta ggtaacgtct
     7201 acgagaatcc agagttattg gaggtagagt aattgccaaa aatattagat gcatgttgcg
     7261 gtagtagatt gttctggttt gataagaaca attctgacgt aacatttatg gattgtcgtc
     7321 agcagtatga agaattatca acaggtcatg tgatcaatgt tgatccagat gtggtcgcag
     7381 actttcgcga tatgcctttt gaagatgatg tgttcgatat ggttgtattt gacccgccac
     7441 acttaagaca tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa ttagacgaac
     7501 tttggccaga agacatcagg caaggttttg cagagtgtat gcgagtttta cgaccttcag
     7561 gaacattaat ttttaagtgg aacgaggaac agataccact ttctgatgtg ttagaggcta
     7621 ttggagaaca gccattgttt ggtaataaac gaagcaaaac acattggctt gtgtttatga
     7681 aaaaattcag ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt attaaataaa
     7741 tgttttggtg gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat
     7801 attgaagtat tcccatacat caataacttt agctatgact cagatgatga atacacgaga
     7861 tacacaggcc aaaagctagg ttcgatggat tttatctact atttcaaaaa agacccgaaa
     7921 atcgacaaag ttacaggaat ttatagcgaa attgaccgac tgtacggaat tgctgatgat
     7981 tccagttttt caagtgattc aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa
     8041 ctaggaagtg aggccagtgg accatatgca agtcttaaag tcgttgatat acccgatggg
     8101 gctgagtggg aaataagtga ttatgacggc gttgaaactg cacattatgg ttttcaaaca
     8161 ggtagtattt agtccactaa tgacaactaa agaggaggca gaaggatgag tgaagatgaa
     8221 tactttgaac agtagtctat taacgacaga gatttttaac tggaaagggg tgtagtcatg
     8281 gacgcatcgg aagtagtaaa taaaattaat agtctcaaag aggttttcgg agacgtcgaa
     8341 gtagtgataa acgcacaagg ctatgacaac gaatttttta agcagattac cgacattagc
     8401 attcagcaag gaatggaaga cgaagacgga aactttatcg atgaagtagc cattttagtg
     8461 acttgcgagt aagtaattga cagctaaaga ggaggaagcg gaatgaaact aaaagacgga
     8521 ttttactcca gcggccacgg catcggcggt ttaatgctag atatgccgac aaagaatcct
     8581 aaaacacgta agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaagga
     8641 ttcatctatc catttcgtgg atatgtagaa cacgtctata atcactcagc aatcattcgc
     8701 attgaaaaca cgatggaatg tgataagtgg ttagcgaaaa gcaaagagaa tttagcagtg
     8761 gtgagattgg tggacatgga agttataaaa tcttaatttt gcttttttac aactatccta
     8821 caacaatgag cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga
     8881 atatagatat taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt
     8941 tagaacgttt ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt
     9001 tgaaattcac aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt
     9061 cggttatcga agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt
     9121 attgttttcc taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata
     9181 gaactatcga gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag
     9241 gagaactcat ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt
     9301 tccgacataa gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa
     9361 gaaatatgag acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc
     9421 tgtgtttctt tcagcatatc tatcagctag atgtagagta gggcaacatc gagaaccaaa
     9481 agtgacagca tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga
     9541 aagaatgctg atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt
     9601 tgttcgaggc tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa
     9661 gaagatattc tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa
     9721 taatactagc gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc
     9781 atcgttagaa ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta
     9841 ccaatttggt ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta
     9901 ttatgatagt gtcgaaaaat taggaaacag gatcgacaaa ataaaatgta agggaggaaa
     9961 tctccctcat cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat
    10021 gtgaatttca actccttcta aattgttctt attatctatc atccgttgct gtctattgtt
    10081 tttttaatta ttcacacgat aactaaaggt gagtgaagag aaatgattcc attaataatt
    10141 tcaatttttg cgctctgtct taatgtctat atgattggat ttaaaaatgg gcaaaataaa
    10201 aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctta
    10261 aaaataaggt caatacgtcg agggatagcc ttaacgtttt atgatttgac catgcaatgt
    10321 tcgattcatt gccagtgact ttggcaaccg agggttggaa atgggcgctc aaagtacacg
    10381 agcaaggcga ggtcgatagt aatcgatgga atcggtgtag gttgcttgat agagctataa
    10441 ctgcatctcg tttatgaggt gtagttttta cagattagat cactctttga gtggtctttt
    10501 tattttacac aaaggaggta acaagaatgt atagaccaca atacttagaa cagaagtatg
    10561 aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga agaccaataa
    10621 agagcgatac atacaaacga aaggaaagca atgaagttat tccattgtat ggcaaaagaa
    10681 tagctaagca ttaaataaga ttgcgaaagg agacggaata tgaccgagga attctataga
    10741 tggctattac agttgataag agaagatcgt ttagttaagt tctatcagtc tcctaaatgg
    10801 cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga
    10861 ctaggtaagt atcatagagt agaaaacgtt catcatataa aggaagtcaa ggatagacct
    10921 gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt
    10981 catggcagat atcttacagc attagataaa caagagaaga agatagaaag ctttgctaac
    11041 ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gataatggca gagagtatga
    11101 tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac gtttgatata
    11161 cgttcgctat gtagatatta gggatctgtt atcagataac tgttgtagta aatacaaagt
    11221 gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa aaaatgtttt
    11281 tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt tcattccgat
    11341 ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg aaacggaggg
    11401 ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta aaatgtttaa
    11461 aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa taagccaatt
    11521 ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt atatcagttt
    11581 atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta gactggagaa
    11641 tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa tgggattgat
    11701 gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat caattcctga
    11761 gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc aaggtatgca
    11821 aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga gaagatattt
    11881 acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac tttccattcg
    11941 aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga tggaaaaaag
    12001 ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat ggtaaaactg
    12061 gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt aaaaattatg
    12121 atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat gatgtattta
    12181 aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg aaggaagtta
    12241 ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct agaacaaaag
    12301 atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa gattattcaa
    12361 tgataaatgt ggctacttct ggtggtggta aaattcgaga ttatagagaa ttttatgata
    12421 caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa tcaaaaatga
    12481 ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt ttgtttccat
    12541 ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg gaaaaagctt
    12601 gtccaactat taattacaat gcagatttaa aacggaaaat gtttcaagaa tactctcaaa
    12661 tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac agacctatgg
    12721 aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa gaaaaagaat
    12781 ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga cgagattttg
    12841 ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa catactttta
    12901 tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat atttctatag
    12961 atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat attgtaggtt
    13021 ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat atgtaccgtg
    13081 caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt gttcgaagcg
    13141 gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt aatcaacgtt
    13201 tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat gaagagcata
    13261 tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa acggatggct
    13321 ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct gctcctgtaa
    13381 cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta gtattctaac
    13441 ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt cacaaaaaaa
    13501 gcaacggtta ctttagaaga atacttttgt aaattgcaag ttgattttgt gtatcgaaaa
    13561 tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc ggaattcaag
    13621 tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa tgtagctcct
    13681 aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt aatattctac
    13741 aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat tgcggatagt
    13801 tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca aattaacaac
    13861 atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata cgcagatcaa
    13921 caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat ttctagtgcc
    13981 atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa tttattccga
    14041 ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca atttaaggct
    14101 tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta cacattagaa
    14161 gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat agacgacatc
    14221 tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg agacatgagt
    14281 gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat tgctgaactt
    14341 attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc agggaatttt
    14401 gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt tgacgctatt
    14461 gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg cgttggaaat
    14521 gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa tcaacaagta
    14581 tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa agaatgaagg
    14641 cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct ttgaagggaa
    14701 tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag aaattgaagt
    14761 agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga acttacttaa
    14821 gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg ctgcttcaat
    14881 tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga tgattcaccg
    14941 cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg accaactgga
    15001 atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca cagatgaagc
    15061 attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt tgagttatgg
    15121 attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta aaaaagaaca
    15181 aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa ttgctgcatt
    15241 tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac agttaaaaat
    15301 ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga tggtaacgaa
    15361 gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa aattttagaa
    15421 gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca gcgtgtatta
    15481 actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt tgcaggggtc
    15541 gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga acaatctcat
    15601 ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg gattgtgtca
    15661 cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa agttttagat
    15721 aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat tcctgtatgt
    15781 aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac tgttttagta
    15841 gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa agatatgcca
    15901 gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga aaaaaaagca
    15961 gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg actatcaaaa
    16021 ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc tgatgtggtc
    16081 ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac tgtactaact
    16141 gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca gtcaacggct
    16201 gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg tgtaggatct
    16261 acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg catttatcta
    16321 gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt attagatatt
    16381 agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac tacaacacaa
    16441 gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg gtttaaatgg
    16501 gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat cgtattgctt
    16561 ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg tgttgaagtt
    16621 catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa accatattta
    16681 tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt ctatatcgat
    16741 tcggatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa agatgatgtt
    16801 tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag ctgtgaaaac
    16861 ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga atctttggaa
    16921 actataaaca ctaattcagg aggcgcacag tgggttatta accgccctaa agcgaattat
    16981 tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg gcaaaaaaca
    17041 aatataaaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat gtatttcaat
    17101 attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc aatagaaaaa
    17161 gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga agatttattt
    17221 ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt tgtaaacaag
    17281 aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg acgcctgaac
    17341 aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc tggaacgaag
    17401 aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa agtaaagtga
    17461 gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc gaaagatgta
    17521 gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt gagttattag
    17581 gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat ggcgaataat
    17641 cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca aactaccaaa
    17701 agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc tttaaaattt
    17761 agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg atcgaaatta
    17821 actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa agatcaatat
    17881 tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga taatttttat
    17941 atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta aagaaaaaga
    18001 acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt ttggcctcaa
    18061 actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt acttcattct
    18121 cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc aaaatctata
    18181 tctaactttc tattcagaaa atagagaaga tttaacagga gattcactag atattatttc
    18241 attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt taaaactaga
    18301 gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat tgaagagtga
    18361 ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa cttcttgtgc
    18421 ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg aaatcaaaag
    18481 gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa gcagatttaa
    18541 gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac attaatttgg
    18601 gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat ttggggatag
    18661 gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt cttgcacttg
    18721 atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag gaggaacaac
    18781 aatggctaaa acaacaacag taacaacttt tgacaatata agtattaaaa gaatttcttt
    18841 taattttaag aatgcaacaa atgcaatttc aactgattgt aatggacaat tagatggcga
    18901 aacagaaatg cagagaattg taaaaaaatg cggttcaaca gaagtaaaat cgaaatctaa
    18961 accaatcaat atgacggtaa caattactgc acatgtaccg atggaagttt atcgacgctt
    19021 caatgggttg aaacaagatg aacgtattaa atcgggaatt tactcttatg gtcctgattc
    19081 cgtaggggaa gatttctctc ttgctgcaga gatcgtggat gatttcgaag aaaaaaataa
    19141 gctgattggt atgttagcat gcacttcgaa tacaggatta acattctcta ttgaaaatgg
    19201 tgcggatgaa gtagctgcgt tagaactaga aacaaaagtt atgcaagatg aatttggtaa
    19261 attttatcat gaagcaattg ttgcagaact tgaagaagac ttaacagatc aatggatgac
    19321 gaatctatct gctgatgtga ttaaaaaaac ttcagttgtg acaactacgg ccactcaatc
    19381 acagtaaaaa aaacggaggt agcgaaatga acgaagatta ctcaaaaatt gaactaaacg
    19441 atggaacaat tttgaattta gaacctaaac tgaatatcaa gaaattattg atgatcaata
    19501 gagattttaa cacagacgag tttgcaaaaa tgtcgatggg aaaaggctct atggatatta
    19561 cagttattca aggtgcaaaa gccgtatatg tagcttatcg tcaagcgaac atggtcgatt
    19621 acatttcatt cgatgaattt atagataaat gagattttga tatggaggtt gcagtagctg
    19681 tatacagtac tatgatgttc aaacaagcac gtgatgccta tcaaaaagaa ttcgaaaaag
    19741 caaataagga aaaaaagctt caaaagtaaa aatgccaaag ctcttagttg aaacgtgggt
    19801 cgatgtctat tcgatgttga ccgacgtttt ttctatgcct tcagatttgg ttttaagcga
    19861 tatctgttta gatgacattt tgcaaatggc ttacaacaag agcgcttatg aaggatggaa
    19921 gaactatgca ataaatcaat cccagaaaaa ctaaaggaaa ggaggtaaaa aatggctaaa
    19981 aagagaacgg aagcagaagt aactttcata gctaacgatg acggattgaa atctacgtta
    20041 aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa
    20101 ttgcaacaga ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag
    20161 cagtatgaat tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgcgaaa
    20221 aaatattatg gagaaaattc caccgaagtt cagaaacttg aaagagaact gattaatcaa
    20281 caaacagcgc aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa
    20341 gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggatag cgaacaaaaa
    20401 aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct
    20461 ggtcaatcag cttctgaagc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa
    20521 tctgaaaatg cggagaaaac gatagatatc ctaagacgac agttagaagc tacacagtct
    20581 gaatttggcg ctacatccac agaagcaatg cagatggaag cgaagcttaa tgatgctgaa
    20641 cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat
    20701 atcggaagca aaatagatat gaataattta atggaagctt ctgacgtttt aagcgacatt
    20761 ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaacag tgtaggtagt
    20821 tcccagagta agatacaagc taattttggt ttgtctaaac aagaggctga agaattaacg
    20881 aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat
    20941 gcattgattt tggtaaagcg taatttagga gatttaaata atcaagattt acaaaatatc
    21001 acggaacaag ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt
    21061 ggtgtaaatg gcttaatggt caatttcggc ttgagtgctc aagatgcaat ggatttaatg
    21121 gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa
    21181 tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa
    21241 aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg
    21301 ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact
    21361 agaaaagctt ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc
    21421 atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa agcttcgaca
    21481 atttggtctg cgcttggcga agataatgcg atgaaagttg tccaatcttt aactgatgtt
    21541 aaccatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact
    21601 ccgttacaag aattaaacgg aaaaatagct gaattaaagg attcattagc tcctataggc
    21661 aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg
    21721 tttaataatc ttccacaacc agtacaggat tatgccgtag cgattggcgg attgactgct
    21781 gcatttactt tattaatgcc aataatagtt ggcttcatgg ctctaggtgg tcctactaca
    21841 ttaataatag gagcagttat tactgctatt gctggagtta tagcaattat aaaaaactgg
    21901 ggcgcaatta ctgactggtt taagggaata tggagtaaat tcactgattg gttgggtggt
    21961 acttgggaaa gtataaaaga aggtgcctca tcagtttggg atggagttaa agaaacctgg
    22021 tctggatttg tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag
    22081 ctatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca agaaacttgg
    22141 caaacattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt
    22201 atttgggcag acattgtagg aattgttcaa ataccatgga cattaataac gtcattgatt
    22261 caagctggta ttaatattat cgtgggtatt tttgatgtag ctggacagtt attaggcgca
    22321 gcttggcaag ctgtttggac acctatttct gatttcctta aaaatacttg ggatactatg
    22381 acacaatgga taagcatcgc ttggaacgga attgtaacta cattccatac tatatttgat
    22441 ccagtagtgg catggtggaa tggtatatgg acatctatta gtactacggc ttcaaatatt
    22501 tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact
    22561 agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg
    22621 ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa tagtgtgact
    22681 agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt
    22741 atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa acactgcttt gaatctttgg
    22801 aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc
    22861 ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt
    22921 gctaacattt gggaaggtat caaaaagact gttagcgctc cgattgattg gattagagat
    22981 aaaatcagtg gcatctttga taatttgaat atttcgatac cacatattcc gttaccacat
    23041 tttaaattga gtggggaatt caatccattg aagggaaaaa tcccaacgtt gggtgttgat
    23101 tggtatgcga aaggtagtgt gtttaattct ccgaatatta tcggtgtcgg tgaagcagga
    23161 cctgaagcag ttttaccttt gaaaagatct gttttgcaag aaattggtga tcgtatcttg
    23221 agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc
    23281 aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc
    23341 attgatagca ttacaaaaat tcaaaatgat aatgcttcgg catggcgtta aacaggagag
    23401 tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt
    23461 ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca
    23521 taccttctcc aaaaagacag agagttgaaa cttctgtttt aggtaggtta ggtggcttag
    23581 tacaagatta ctcgtttgaa gatatgtcgt ttacattgca ctataactat ttagaggatg
    23641 tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaactatg
    23701 caaagaaatt agaattctct gacgatccca acgtctatta cgttatccag actatcgata
    23761 ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga
    23821 aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta
    23881 acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta
    23941 cttcatgcca gttcatctta aatgattatg tgtttagttt cgaaggctta gtagcaggaa
    24001 ctgatgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata
    24061 ttttagatcg gtccaacaaa atgaagacca tgcaatatcc aacgttgcaa gtggatatta
    24121 atcattttaa ttgtactggt ttgagcaaaa tacaaattta tcgtaacggg ttaaggtagg
    24181 tgaaatagat gatcgataat ttaataacta tttacgataa aaatgacgcg aataatttag
    24241 ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg
    24301 ttagcaataa actaaacgga gccgagatat ttcagggtac ttattcaata aacggaacta
    24361 atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa
    24421 aacagcgtct acggatttat tatgcaaaaa cttctgtaat aggcaatacg atagaagtaa
    24481 aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat gacagcggaa
    24541 cagaaaagat cactgctact caggcatggc aaaacgcaaa agttttagcg aaaccagcta
    24601 tcccttcgca gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa
    24661 aggcgaattt tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg
    24721 gtgagtttct aaaagataat aacacattac gtcatgaaaa aagtctaggt acggatcata
    24781 aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa
    24841 gtgttttagt tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca
    24901 ctctaccaga agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt
    24961 cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag
    25021 actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag
    25081 taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg
    25141 gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg
    25201 aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa
    25261 caaacttctt agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc
    25321 aattgaaaaa tggtggcgaa atcagtgatt taatcaatga tattgtagat catcaaactg
    25381 atatgattac tggtcaaaat ggtggttacg ttttattaga tcctaaagaa gcccctagtc
    25441 gtattttgat tatggacaca ccagataaga atactgcacg gaacgttttg caaatcaaca
    25501 acgctggtat tggtttctct aaaactggca ttaatggaac gtatgacacc gcatggacgt
    25561 tagatggcgg attcaatgcc tcgtttatta cagctggtga gatagtaggg attactatta
    25621 gaggtactac attaattagt gatggcactg attatagaac aagtatagct aatggcaaaa
    25681 tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg
    25741 taagtgctga tgcaggtatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta
    25801 gaaatccaca ggggaatttg gtttttagta cgtgggataa tggtaataac aaaccgtttt
    25861 tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatataact gatggcgatg
    25921 gtagttcttt aagcattaat ggtagtgcgg gcagttcatg ggagtttaaa gtagctggta
    25981 ggactatgaa atttactagt gatggtatgc taacgttgcc aggttgtttt tttggttcat
    26041 gggaagatgg aaaacttgct aggtttgaac aatcaacggt acaagtatat aaaaatttta
    26101 ctgttagagg tactaaaaac tcaaccgtac caacagaaca ttatggacaa cgactattaa
    26161 acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgatg
    26221 atggtaaagt tcgtgttgat attgatccta tgtttgccga gacagtaaat ctaagtcgat
    26281 atatgacaca tgtgacacct acagagctag ttttgtgtgc tgttactcat gaagatattg
    26341 accatttcat cattgaaact agtaagccaa acgtattggt tagatggaat ttagtggcac
    26401 atcgactagg gtatgaagat gttagattaa aagaggatac agcatatgat agcacagtgc
    26461 ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata
    26521 taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca
    26581 aggtgacaaa ggaattacgg tgactgttaa accgttttta aatggcttgc agatggatac
    26641 gagtggcgga acctttacat taaaaggaac aacaccatct aaccgttacg tagatagtgt
    26701 cgctactagt ataactagcg aagaagtcac attttctctt gatggaacat ttatgagtga
    26761 agcaggatac tataaacact gctacgtaga atatagaaaa gataatcaaa ttttaacgac
    26821 gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga
    26881 atatgtttcg caattagaag aattgattcg aaagtataac gaaacttttg atgcttttct
    26941 ggctgaaatt aaaggtagaa tggatagctt aaataaacag attactgatt taactggtca
    27001 agctaaaaca ctacaagaca agttagatgc tctgaaagaa gaaatttcta aattaggtaa
    27061 cttacatgtg atgtactcca acagcatcga cttcgggggc tatgattata gtgggaatcc
    27121 gaatttaatg tccaaactaa aatcgagcga ttttaacgtt ggttaccacg ggtcactaac
    27181 taatgttaac ggaatgttac attttatgtc tgatggtaca ggggacattg atatgtttac
    27241 gcgtattaat acacctcagc ttgctagtgg gaaaacctat actctgagtg cgaaagttcg
    27301 atttgatgaa ggaactacag gagctattga taaattacgt ttggtgtatc gtacatcacc
    27361 aggagaaaag atattattgg aagcaaatag tacaaatatt acaacagatg atgtagggaa
    27421 agaaataaca atcaaaggta cagctaacgt taattatcaa atcacaaatt tagatcgatt
    27481 ttatattagt gttagttttt ctaacaggga taaaataaat ggtggattta aattgtacga
    27541 catcaaaatc gaagaaggct caacagccac tccgtatcag ccaaacttac tagatgaccc
    27601 ttactgggta ggtaaaacat cgttgggtaa gaatattgca aataaaggta cgacgttccc
    27661 aattaaatct agcgcgtacg aaatatataa aggtaacacg gaagaagagc ttataatagg
    27721 tcaaacgtat actatcacgc ttaaaggaac aaaacccgca agtcaaacct ttgtagcata
    27781 taattattgg aacgttaatt ttggagatct aaaaccagtt gagggattga cagacgtatg
    27841 gtctctaaca ttcacaccaa cgaaacttga accgggtttg cctaaagacc ttcgcatttt
    27901 tcagtcgcct aaagaaacgg ccggcgcatg ccgaattgac tggctcaaga tcgaaaaagg
    27961 cgacacacga accccgaata ttgagcaata taaataccga ggaatcggca tgcgagactc
    28021 aaacaatcca aaagattatg tatgggatct agaacctagc tatgttgaga ataacctagc
    28081 tacagatatc aaggttgctg aaatcacagg taaagcaaac aattataccg atgggaaagt
    28141 atcggagatt aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga
    28201 tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga
    28261 tgaacgcatt aatgatacag ctactaccac attaacagtt acaaacggga ataccggatc
    28321 agcaaagctt tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaaa
    28381 acgcagtggt ggaaatgatt caacgatact aacaattcca gaaggctatc ggccaccaat
    28441 tagttttgag caactggttg gctcgataga ccgttctact ttgaacagtg ctcagttatc
    28501 tattggtgca gatggagcca ttaaatggcg aagaaactca agttatggat cggattatac
    28561 ctttgcaatt acttacacga tttagaaaag cgtgaatcga tatgaaggca gcatatagac
    28621 caattgaacc ttacggattc gagcaaatca ttgtgaatga tgaagaacat ttaccggaag
    28681 aatgcacaga agtcgaacca ccgattccaa attggaaacc gaaattcaat tactgggagg
    28741 gaaataaatg aaaaacattt ggaaatatgg acgtactggc ggagagtacg caggaaaagt
    28801 attggacgac atgcttgtat ccgttcctta cacagatcag ccaccgcttg aaggaattcg
    28861 tgctgatggc gaaccgttaa caatcgctga tcaaatgttt gatcctaaat tgaatcaatg
    28921 gattatttta gcgaacgcac tagatcacaa cgatttaaac aatctcaaag caatgtatga
    28981 gtcgttagaa aatgagaacg gcgatttaaa acagatcaat gccaaactca tgctaagcga
    29041 tgtagcaatt aaacaggaaa atactgcatt gaaagaaaaa gcggatagtt tagcacaaat
    29101 caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag aaattaaaga
    29161 gcaactaaat ccagcttcaa agggaggtga gtagtatgtt tagttttagc gatgtgaaaa
    29221 tgatgtatga ttggggctgt ttcactaacg aacaagtaat ggttttcgtt ccgttgtgca
    29281 ttactgaaga a
//
LOCUS       DAZBWR010000022        27937 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000022 DAZBWR010000000
VERSION     DAZBWR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.022"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..122)
                     /locus_tag="ACHGT5_000916"
     CDS             complement(<1..122)
                     /locus_tag="ACHGT5_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN7216921.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEK"
     gene            complement(124..321)
                     /locus_tag="ACHGT5_000917"
     CDS             complement(124..321)
                     /locus_tag="ACHGT5_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN7216922.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(715..2442)
                     /locus_tag="ACHGT5_000918"
     CDS             complement(715..2442)
                     /locus_tag="ACHGT5_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN7216923.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2435..3628)
                     /locus_tag="ACHGT5_000919"
     CDS             complement(2435..3628)
                     /locus_tag="ACHGT5_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN7216924.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3815..4459)
                     /locus_tag="ACHGT5_000920"
     CDS             complement(3815..4459)
                     /locus_tag="ACHGT5_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7216925.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4553..4687)
                     /locus_tag="ACHGT5_000921"
     CDS             complement(4553..4687)
                     /locus_tag="ACHGT5_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN7216926.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4689..6821)
                     /gene="feoB"
                     /locus_tag="ACHGT5_000922"
     CDS             complement(4689..6821)
                     /gene="feoB"
                     /locus_tag="ACHGT5_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN7216927.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6818..7291)
                     /locus_tag="ACHGT5_000923"
     CDS             complement(6818..7291)
                     /locus_tag="ACHGT5_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN7216928.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7692..7916
                     /gene="nrdH"
                     /locus_tag="ACHGT5_000924"
     CDS             7692..7916
                     /gene="nrdH"
                     /locus_tag="ACHGT5_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN7216929.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8075..10234
                     /gene="nrdE"
                     /locus_tag="ACHGT5_000925"
     CDS             8075..10234
                     /gene="nrdE"
                     /locus_tag="ACHGT5_000925"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN7216930.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10284..11249
                     /gene="nrdF"
                     /locus_tag="ACHGT5_000926"
     CDS             10284..11249
                     /gene="nrdF"
                     /locus_tag="ACHGT5_000926"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN7216931.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11338..12477)
                     /locus_tag="ACHGT5_000927"
     CDS             complement(11338..12477)
                     /locus_tag="ACHGT5_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN7216932.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12786..13499)
                     /locus_tag="ACHGT5_000928"
     CDS             complement(12786..13499)
                     /locus_tag="ACHGT5_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN7216933.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13753..14454)
                     /gene="nagB"
                     /locus_tag="ACHGT5_000929"
     CDS             complement(13753..14454)
                     /gene="nagB"
                     /locus_tag="ACHGT5_000929"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN7216934.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14688..16220
                     /locus_tag="ACHGT5_000930"
     CDS             14688..16220
                     /locus_tag="ACHGT5_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN7216935.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16411..17613)
                     /locus_tag="ACHGT5_000931"
     CDS             complement(16411..17613)
                     /locus_tag="ACHGT5_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7216936.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17695..18384)
                     /locus_tag="ACHGT5_000932"
     CDS             complement(17695..18384)
                     /locus_tag="ACHGT5_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN7216937.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18490..18858)
                     /gene="rplL"
                     /locus_tag="ACHGT5_000933"
     CDS             complement(18490..18858)
                     /gene="rplL"
                     /locus_tag="ACHGT5_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN7216938.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18911..19414)
                     /gene="rplJ"
                     /locus_tag="ACHGT5_000934"
     CDS             complement(18911..19414)
                     /gene="rplJ"
                     /locus_tag="ACHGT5_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN7216939.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19618..19745)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19824..20513)
                     /gene="rplA"
                     /locus_tag="ACHGT5_000935"
     CDS             complement(19824..20513)
                     /gene="rplA"
                     /locus_tag="ACHGT5_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN7216940.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20617..21039)
                     /gene="rplK"
                     /locus_tag="ACHGT5_000936"
     CDS             complement(20617..21039)
                     /gene="rplK"
                     /locus_tag="ACHGT5_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN7216941.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21382..22050
                     /gene="sdaAB"
                     /locus_tag="ACHGT5_000937"
     CDS             21382..22050
                     /gene="sdaAB"
                     /locus_tag="ACHGT5_000937"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN7216942.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22063..22935
                     /gene="sdaAA"
                     /locus_tag="ACHGT5_000938"
     CDS             22063..22935
                     /gene="sdaAA"
                     /locus_tag="ACHGT5_000938"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN7216943.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23026..24126)
                     /locus_tag="ACHGT5_000939"
                     /pseudo
     CDS             complement(23026..24126)
                     /locus_tag="ACHGT5_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24218..25105)
                     /locus_tag="ACHGT5_000940"
     CDS             complement(24218..25105)
                     /locus_tag="ACHGT5_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN7216944.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25182..25727)
                     /gene="nusG"
                     /locus_tag="ACHGT5_000941"
     CDS             complement(25182..25727)
                     /gene="nusG"
                     /locus_tag="ACHGT5_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN7216945.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25828..25998)
                     /gene="secE"
                     /locus_tag="ACHGT5_000942"
     CDS             complement(25828..25998)
                     /gene="secE"
                     /locus_tag="ACHGT5_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN7216946.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26022..26174)
                     /gene="rpmG"
                     /locus_tag="ACHGT5_000943"
     CDS             complement(26022..26174)
                     /gene="rpmG"
                     /locus_tag="ACHGT5_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN7216947.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26336..26980
                     /gene="cbpA"
                     /locus_tag="ACHGT5_000944"
     CDS             26336..26980
                     /gene="cbpA"
                     /locus_tag="ACHGT5_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN7216948.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27036..27539)
                     /locus_tag="ACHGT5_000945"
     CDS             complement(27036..27539)
                     /locus_tag="ACHGT5_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN7216949.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27552..27598)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         8452 a         5779 c         4951 g         8755 t
ORIGIN      
        1 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
       61 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      121 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      181 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      241 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      301 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      361 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      421 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      481 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      541 tctttgactc gcacccataa attcataata gtttgtcggc gtctttggac gtttcgaaga
      601 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
      661 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
      721 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
      781 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
      841 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
      901 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
      961 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1021 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1081 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1141 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1201 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1261 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1321 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1381 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1441 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1501 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1561 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     1621 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     1681 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     1741 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     1801 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     1861 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     1921 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     1981 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2041 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2101 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2161 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2221 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2281 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2341 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2401 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2461 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2521 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     2581 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     2641 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     2701 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     2761 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     2821 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     2881 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     2941 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3001 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3061 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3121 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3181 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3241 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3301 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3361 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3421 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3481 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3541 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     3601 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     3661 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     3721 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     3781 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     3841 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     3901 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     3961 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4021 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4081 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4141 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4201 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4261 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4321 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4381 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4441 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4501 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4561 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     4621 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     4681 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     4741 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     4801 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     4861 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     4921 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     4981 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5041 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5101 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5161 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5221 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5281 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5341 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5401 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5461 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5521 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     5581 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     5641 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     5701 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     5761 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     5821 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     5881 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     5941 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6001 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6061 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6121 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6181 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6241 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6301 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6361 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6421 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6481 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6541 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     6601 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     6661 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     6721 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     6781 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     6841 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     6901 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     6961 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7021 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7081 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7141 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7201 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7261 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7321 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7381 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7441 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7501 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7561 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     7621 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     7681 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     7741 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     7801 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     7861 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     7921 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     7981 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8041 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8101 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8161 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8221 ttttctatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8281 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8341 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8401 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8461 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8521 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     8581 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     8641 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     8701 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     8761 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     8821 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     8881 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     8941 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9001 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9061 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9121 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9181 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9241 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacgg atatctcatg
     9301 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9361 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9421 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9481 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9541 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
     9601 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
     9661 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
     9721 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
     9781 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
     9841 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
     9901 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
     9961 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaatggcac aacaacacgt
    10021 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10081 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10141 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10201 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10261 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10321 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10381 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10441 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10501 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10561 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    10621 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    10681 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    10741 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    10801 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    10861 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    10921 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    10981 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11041 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11101 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11161 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11221 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11281 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11341 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11401 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11461 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11521 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    11581 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    11641 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    11701 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    11761 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    11821 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    11881 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    11941 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12001 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12061 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12121 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12181 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12241 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12301 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaactg ccgaaagcat
    12361 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12421 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12481 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12541 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    12601 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    12661 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    12721 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    12781 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    12841 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    12901 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    12961 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13021 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13081 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13141 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13201 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13261 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13321 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13381 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13441 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13501 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13561 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    13621 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    13681 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    13741 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    13801 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    13861 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    13921 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    13981 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14041 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14101 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14161 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14221 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14281 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14341 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14401 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14461 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14521 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    14581 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    14641 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    14701 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    14761 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    14821 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    14881 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    14941 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15001 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15061 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15121 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15181 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15241 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15301 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15361 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15421 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15481 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15541 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    15601 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    15661 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    15721 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    15781 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    15841 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    15901 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    15961 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16021 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16081 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16141 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16201 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16261 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16321 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16381 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16441 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16501 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16561 tccaataaag tacataaaca taaatacagt attatttcgg ttgcttgctg cttcacctag
    16621 cccttgtact cgtgtttgat tggcgacttg accaaattga ttcccgacgt ctaataaaat
    16681 aatagctaaa agcaaaatgg ccaaatggct tccgccaacc aataaagcaa taaagcttat
    16741 tgtttgcata acaagtccca tatacacgat ttttctttca gaataagaat ctgccagtcg
    16801 tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa atcgctaata tccctgcttc
    16861 gaatgttccc caattgtagg cgggactgct gatataaaaa atcaacgtag accaaaagat
    16921 cgaaaatgtt ccaaacatga aaaatccact gactgccgct tctcttaata gacgttgaga
    16981 agcaactaaa tgcggcaaac ttttaagtga atcaaggtat gaaacagctg tactactttg
    17041 ctggacagaa cgcggcattt tccagtaaag cagaagtgta agaattccga ctgcacataa
    17101 agcaaataaa tagatcatcc gccaagaaaa aagactggca acaataccag ataccgtccg
    17161 agaaagcaaa atccccacga gtaaaccgct aagcaatgta cccatgacct tccctcttgc
    17221 ttttggtccc gctagtaccg caccgtaagg aatgatgatt tgcggcacga tcgaaagtaa
    17281 tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa ggcgcaaaaa aagcggccaa
    17341 cagagagacg gcagataacg ctgccattcg aatgatcaat tttggacgat ccaccacgtc
    17401 ccctaaagga actaaaaaca gtaaccctaa cgcatagcct aattgggtca acatcgtcaa
    17461 tatcccgact gcacttgtgc taacagataa actagctgct acttttgttc caatcggctg
    17521 gatgtaatac atattggcaa caacaacgcc acaagtgatt gataatagta aaataagcga
    17581 agttgttaaa ggtttttttt cattactctt cattgatttc ccccatttcc tatcttagtt
    17641 tacaacttat ggaaaagatt ggaaatagaa aaatacaact attcgccaca cttattattt
    17701 gtgcttttgt tttccttgtt ttttcttgcg atctttataa atgaagacaa ctaaccctac
    17761 tagcacagta acacttgcaa ccaacgtgac tgcagttttg ttatatccgt gaaacagagc
    17821 ttctccacca cacgcataca acacagacga agggatgact ccaatcacga tcgacaatat
    17881 gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa ttgaccatag aagatggaat
    17941 aaccggtatc atatagccta acattacacc tatttttgga tgtttcattt gtcggattgc
    18001 ttgcacccaa tgattcgttt cggttttctt atctaaaaat tttaagcgat gcataaggaa
    18061 aattgcagtg atgttaccaa gagcattacc caaaatgttg atgatcgcac ctgtcagtgg
    18121 tccaaagctc aaaccagcaa tgactccaac tactgaagta ggaatacctg gaacagcaca
    18181 catcaccgct gtcaatacga tcaagagtat cgatgcaaaa gtcccatgcg aacgaatatg
    18241 tgcgagtagc tctgccttac tcgctctcgg atttaaaaat aattggatgt ctgtctgata
    18301 ttctaaatac aaccgatagc ccactaccag tatcagaatg attcccaacg agatcaatat
    18361 caatcggatt attttctttt tcatatttcc tcctgaaaaa aatctatatt cattaaataa
    18421 tagtttaaca taaaaacaaa aaagcaccga aatccaacat aggatctcag tgcttttaaa
    18481 tagtaaaaat tatttaacag ttacagatgc gccaacttct tcaagtttag ctttcaattc
    18541 ttcagcttct tctttagata cgccttcttt aactggtgca ggagcgccat caactacagc
    18601 tttagcttct ttcaagccta aaccagttgc ttcacgaact gctttgataa ctttaacttt
    18661 ttggtctcca gctgcagtta attctacagt gaattctgtt tgttcttcag cagcagcagc
    18721 gccaccagca gcagctacag ctacaggagc agcagcagat acgccaaatt cttcttcaat
    18781 agctttaact aggtcgttaa gttctaggat agttgcttct tttaactcag caacgatgtt
    18841 ttcaatgttc aatgccatta taatttcctc cgtttaattg ttctttttta tttaatgctt
    18901 tatgcggcaa ttaagctgca tctccatctt ctttgtctgc cactgctttg acagcgtaag
    18961 ccacgttgcg gactggcgct tgtagtacag atagcaacat agatagaagt ccttcgcgat
    19021 ttggcaattt tgccaatgct gtgatttctt ccaatgaaga gactttacct tcgatgatac
    19081 cgcctttgat ttctaatgct tttgcttctt tagcaaattc gtccatgatt ttcgctggtg
    19141 cgactacatc ttcgttactg aatgcaacag ctgtaggtcc agtgaatact tcgtccatgc
    19201 cttcaaggcc agctttttta gctgcacgtg aaagaataga atttttgata actttcattt
    19261 ctacgttagc atcacgaagt tgtttacgta aacgtgttac ttcatcaact gttaatccgc
    19321 ggtagtcaac gactactact gatgcagctg ctgtaaattt ttcgcttact tcatcaacga
    19381 ttgctgcttt ttttgcgata attgcttcac tcatttttga gtttcacctc ctgaattttg
    19441 atggaagagt tttttgcact gaaaaggcaa gtcactttta tagttacttt gctcaaccag
    19501 tattattcat cacctattca cttcgttcat agtgattcta ctcgctttgc tcgaaaggaa
    19561 actcctttcg ctttgctcaa ccagtattgt tcatcatctc ttcacttcgt tcatagtgat
    19621 tcacaataaa aaactctatg ccaccgcaga catagaggga ctattgaata tattcaataa
    19681 acgtcctcgg taggaaatta aggcttgatg ccacctactg tcttcggtac aagttaatta
    19741 ttaacctcaa ctagcttagc atgctaaact agttgagtca agaattattt ttcagttgga
    19801 tcaatcaaca ttctaatatt taattagaaa gaagcttggt caacatggat accaggtcca
    19861 aatgttgttg tgatcgttac atttttcatg tattgacctt tagctgctga tggtttagct
    19921 tttagcaata catcatggat tgctgcaaag ttttcaacta atttttcatc gtcaaatgaa
    19981 acttttccga ttggtacatg gatatttcca gctttgtcca cacggtaagt tactttacca
    20041 gctttcactt cgtttactgc ttttgttacg tccattgtaa ctgtaccagt tttagggttt
    20101 ggcattaagc cttttggtcc taatactcgt cctagacgac ctacagtagc catcatatca
    20161 ggtgttgcaa caactacatc aaagtcaaac catccgtttt ggattttttg aaccatgtcg
    20221 tcgtcaccta cgaaatctgc accagctgct tcagcttctt ttgctttgtc tcctttagca
    20281 aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta atacaacagc accacggatt
    20341 tgttggtctg cttttttagg gtctacgttc aaacggtatg caacttcaac tgttgcatca
    20401 aattttgcaa tgtttgtttc ttttgctaaa gctactgctt cggcaacagg ataaacttta
    20461 cttgaatcaa ctttttttaa tgcatcttgc atttttttgc tctttttagc cattttggtt
    20521 cctccttgat tgtggttata acggttgtac ctcccacgtg ttctatatct ttcaatatag
    20581 agccaactga gaagctgctg tcttagggtg tatagattat tctacagtga tccccatgct
    20641 tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg ttagctgcgt ttaggtcttc
    20701 catttttaat tcagcgattt ctcttacttg atcgctagaa actttcgcaa ctttattttt
    20761 gtttggttcg cctgaacctt tttcgatttt agctgctttt ttcaatagaa cagcagctgg
    20821 cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat acagaaataa caaccggaat
    20881 gatcaaacct gcttgatcag ctgtacgagc gttgaattct tttgtgaatc ccatgatatt
    20941 gatacccgct tgacctagtg caggacctac tggtggagct ggagttgctt tacctgcagg
    21001 aatttgcaat ttaacgattt tttctacttt ctttgccacg agacatacct ccttaagtcc
    21061 gtgatgtggt taattggaga tgcatatttc tcctcccacg ttttatctga tctgaaaaaa
    21121 tccagacaaa taatgtgcaa aacaatgcac actggtacag tatagcaaat ttatttgttt
    21181 tttcaagatt tttttctgct atttttacat tttgcttgaa taaacgctaa atttattcta
    21241 aaaaaacaac aatttttcgt tctcatttct tcttttatgt tacacttgcg ctagcatggt
    21301 tcaacagatg aaagcaaaca ataacacggt tttttagacc cttgtacaaa gattcctaca
    21361 taacaaaatg aggtggagaa aatgaaagat ttaaggtata gaagtgtctt tgatattatt
    21421 ggacctgtga tgattggtcc aagtagctct cacactgccg gagctgctag aatcgggaaa
    21481 atcatacgta gtatatttgg cgaacagcct gattctgtag atatttatct atatgaatca
    21541 tttgcaaaaa cctatcgggg gcacggaaca gatattgcat tagttggcgg cctgttaggg
    21601 atggaacccg atgatgaacg tttagctgat tctttgaaat tggcatatga agccggtatg
    21661 gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt aaagatgctt
    21721 ctaaaaaaag gaacacgaaa gctttcagtc actggcattt cgatcggcgg tggcaatatc
    21781 cagatttctg aattgaatgg attcaaaatt tctttaagca tgggtactcc taccttgatc
    21841 gttgtccacc aagatgttcc aggtatgatt gctaaagtga ctagtatact ttctgagacc
    21901 aatacgaata tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa tgccattatg
    21961 ataattgaag tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa acagctgcca
    22021 aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt atcgattgaa
    22081 gaattagtag atcaagcaaa agcttatcca agtgtagcag aattgatgat tgcagttgaa
    22141 atggaaatga gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa aaacctctca
    22201 gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac aggaattacc
    22261 ggtggtgatg caaaaaaatt agatgattat ttaaatgagg cagacttttt aagtggagaa
    22321 acaatcttaa aagctgtacg caatgctgta gctgttaatg aagtcaatgc aaaaatgggg
    22381 ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt gctgatggct
    22441 gtccgagatc ggcttgattt gacacatgaa cagcaattgg aattcttatt taccgctggc
    22501 gctttcgggt tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg gggatgtcaa
    22561 gcagaagttg gttctgcaag tgcaatggca gcagcagctt tggtttgtgc aaaaaatggg
    22621 actcctcatc aagcagcaca agcagtagcc attacattga aaaatatgat gggactgatt
    22681 tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc tttaggctct
    22741 tctcaagctt ttatctctgc tgatatggca ttagcgggta tcgaaagtgt cattccgccg
    22801 gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat tttcaaagaa
    22861 acagcagaag gtggcttagc tgttacgcca actgctaaac gacttgcgga agagatttta
    22921 aataagaggg cgtgataaag ctccttaatt tcgagtatta agagattagt tatcttcata
    22981 acaaaaaacg aagagaggac taattgccct tctcttcgtt ttttattaat ccatcaattt
    23041 cgtatttttt acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga tcgacaattc
    23101 tttgttcagg tgttcaaaaa cagatgttac tccttctgcc ccacctagat tcaacccata
    23161 aatcactggt cggccaatcg caactaagtc agcaccactt gccagagctt tgaagacatg
    23221 ctctcctcga cggacaccag agtcgaaaat cactggtaca cgtttgttga ctacttcggc
    23281 aatttttggc aatacttcaa aggacgcagg acctccatct agttgacgtc caccatgatt
    23341 ggatacccag ataccatctg cccctgcaga gatagcgatt actgcatctt ctggcgattg
    23401 tattcctttt acgataactg gaagatgggt gatttctttg attgttttga tgtcatctgg
    23461 tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt ccttttcctt cgccattacc
    23521 acttgcactt tgttcgctat acgcagctaa gttaggcatt ggtaatggaa attggaattg
    23581 attgatgacg tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta aaatgatagc
    23641 tttagcccct gcttctactg ctttgttcaa aatgaactca ttgaatctgt cgtctttact
    23701 catatataat tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt ctttgattgt
    23761 cgtatttgca taagtgctga tggaaaagat tgatccagca gcagctactc ctttagctgt
    23821 atcagcttcc cctttttcat gtgccaatcc ttgggccgct gatggcgctt ggataatcgg
    23881 tgtatctaat tcaattccga aaacagatgt atgcaaatcg gctgagtcga ttccgcgtaa
    23941 tatccgtggc atgattttct ttgtattaaa agaagtcgta ttttctttca tcgtccactc
    24001 atcttcagaa ccaccgcgaa tatagccaaa agctcctgct tccatgcgct cttttacacg
    24061 accttctagt taggcaatgt ttacaatatc gattggatgt tcttcattac ttgtatgata
    24121 agtcatagta gatccttctt tcatcttctt gattacatat aaatcatact aaacttacaa
    24181 aaagtaagca aacatttact ctcagatact tttatcgcta ttctttccaa ataaatcgac
    24241 ttacaagctg atctacttct tggttttcat gaagcatact atgagaggca ttttcacctg
    24301 tcactacttt ttcctctatt tcatttccgc gttgcttcaa caacgcatag accgctaatg
    24361 cactgtttaa cggtactgtt ccatcactca agtctgtttc atctagttga ccagcaatca
    24421 agaaaaatcg agtggttata ggaatattac cgatcatttg ctggtaatct tgatagcggt
    24481 tagaaacgac agcaggcccc tttgctaact catctgtcag agactgcgca ctatcatcga
    24541 caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc aatgacgtat
    24601 cctgtccata cgttcctaaa tagcgtagtg cactaactcc tcccatggaa tgccccacta
    24661 tgttgacttt atctatgccg tagttttctt tcagatacaa aagacaagtt ttgatccatt
    24721 ctgcttgatt ccactcgtta tttttattat cttcaaacaa tacttggacc atcggattgt
    24781 ctgcttttcc agacaaatct cctgttgctt gtatttgtcc atctgttcct actgttaaaa
    24841 ccaattcttt tactgcttgc tgattttttt ccaaacgccg caacatccct ttaaaggaat
    24901 tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat agtggttttt tctatcactt
    24961 tactggattc tgccgttttt tctttatcag aaacttcagt agtatttatc ttcgtcgtct
    25021 ggcaaccact taacgcaaac aaactgataa ggactaaagc tgctgcagtt gcacgtttgc
    25081 ataaattttt gattggttct gtcatacacc tcttcctttc catatagaaa aaagaaagac
    25141 ttgaaaggcc aatcatatga tcgacatttc aagtctcttt attaatcgat tttatctact
    25201 tgatcgaaat caagttctgt actggtttca cgtccaaaca tatcgatgtt gactttcaat
    25261 ttttcttttt ctccgtcaac ttctgttact tgtccttcta atccagcaaa tgcgccttcg
    25321 atgattttca cgacttcgcc aacttctact tctagatcat tttgtcgcag gctcataccg
    25381 atagaacgca agatatgatt gacttcttct ggaagtaatg gtgctggttt gcttccggct
    25441 ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc ggacaacata ccatgaagcg
    25501 tctgtcatga tcatttcaac aagtacgtat cctgggaatg ttttatgaac gatttctttt
    25561 tctttcccat ttttgacttc agtttctttt tcttcaggaa caacgacacg gaaaataaag
    25621 tcggccattt tcatactttg cgcacgagat tcgatatttg cttttacttt gttttcatat
    25681 cctgaatatg tatgcaagac ataccagttt ttttcaaaag attccattga aggctcctca
    25741 tttcattgta aaataaaaaa acctcagtac acccgaagtt tttctttcgc ttcattatag
    25801 cactaaaagt acgttgtggc tagcgagtta ttttaaaatc cagccaaata agctttggat
    25861 ggctgtatcc attacgtaaa acatcaatcc gaaaataata gaagtttcga tgactactaa
    25921 cgtatcttta cggagttgtt ttctgcttgg ccaagttact tgtttgattt catcttttac
    25981 gctttttaga aatttcatga gacaacctcc cttgctactg tttatttcgt ttctttatgt
    26041 aatgtatatt gattacaata tttacaaaac ttattgattt ccaaacgttc gccgcttttt
    26101 ccttcactga ctgattttgt gtagttgcga gacccacaaa cggaacaagc gagtgctgct
    26161 tttttctttg ccatttactt ctgccccctt gctgagaatc agacttatac ttcataacag
    26221 tatcacgtaa ctttttactt gtcaatttgt gaaataaact atctattgtt ttggataaaa
    26281 ttatgatatg attaatttga aattaagcta aaatgagaca attggaggta tagcgatgct
    26341 attaaaaaca cttgtatata agaaaaaaga tctgacaacc gttacagaga ctagtacatt
    26401 agaagaagca ttaaaaattt tagaaacttc cggataccgt tgtgtgccga tcttagattc
    26461 ttcaggtaat attttccgtg gaaacattta caaaatgcat atctatcgtc ataaagcaaa
    26521 tggcggtgat atgacgcttc ctgtcactca tcttttgaaa aacgctacaa agttcattta
    26581 cctggatact tctttcttca aagtattctt tacgatcaaa gagcttcctt atattgctgt
    26641 ccttgacagt gaaaatcatt tttatggtat tttgacacat agtacgttgc ttaatatgct
    26701 ctctcaatct tggagcgtag aacaaggaag ctatgtactg acaattgctt ccacaggaaa
    26761 gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa cacagcagca ttgccagctg
    26821 tattactctt gatatcgaaa aagacgaata cattcgacgt acattgatca cgctaccggc
    26881 taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa agaattataa
    26941 agtggtagaa attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg aaaaaagaaa
    27001 acagctaatc tgctttcttt tttcgctttt tataattaga cacgcttttc aaaatcaagc
    27061 gcgcgatcta aataatacat caatggtgct gaaacagcca tgacgataaa ctcacttaat
    27121 gcaagcgtgc cataggttgt ccaaaatgct tggccacctg ttggaactaa catataagca
    27181 atcagtccca tgctgatggt aaagaagatc gtgttcaata ctaaacgcat ctttacattt
    27241 ttcactttgt tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg gccgccacca
    27301 aaaaccacat ccatgatacc aaaaccaaag aaggcattat agacgatgac cccgcctaaa
    27361 actccccaaa gcaatttacg gttaaagaca actaaatgat tcaaactttc tgaaatacga
    27421 aactggatcg gccctgagga tacaggcgca actaaaatag ttaacgctag ataaagagcc
    27481 atgatgatgc catttacaac gattacggat aaccgttgtc tgcttttcgt tttttgcatt
    27541 ttctctcctc ttttctccta gtttttttaa cgcgggatgg ttgatgaacc gcggttatgt
    27601 gaaacaaaca ataattataa cagacttttc atttatttca atccgtctta tctgctttta
    27661 aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata tggtataaag
    27721 taagtgtaag gggtcataag aattcccagt aaagcagacg aagtaggatc tgcaagaaca
    27781 gaattgttgt gaaacttaaa taaataaaaa aggatgtggg aatttgagca acctcttact
    27841 agagaacggt ctagcaagca gctgaagatc taagtggaca tgccgaacct tggaacttct
    27901 ccttacgtaa gtaatggatc atgcgcttgc taggctt
//
LOCUS       DAZBWR010000023        27582 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000023 DAZBWR010000000
VERSION     DAZBWR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.023"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..714
                     /locus_tag="ACHGT5_000946"
     CDS             67..714
                     /locus_tag="ACHGT5_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN7216950.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            798..1742
                     /locus_tag="ACHGT5_000947"
     CDS             798..1742
                     /locus_tag="ACHGT5_000947"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN7216951.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1877..3328
                     /locus_tag="ACHGT5_000948"
     CDS             1877..3328
                     /locus_tag="ACHGT5_000948"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN7216952.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3349..4185
                     /locus_tag="ACHGT5_000949"
     CDS             3349..4185
                     /locus_tag="ACHGT5_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN7216953.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4185..4844
                     /locus_tag="ACHGT5_000950"
     CDS             4185..4844
                     /locus_tag="ACHGT5_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN7216954.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4970..5659
                     /locus_tag="ACHGT5_000951"
     CDS             4970..5659
                     /locus_tag="ACHGT5_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216955.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5665..6621
                     /locus_tag="ACHGT5_000952"
     CDS             5665..6621
                     /locus_tag="ACHGT5_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN7216956.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6665..7177
                     /locus_tag="ACHGT5_000953"
     CDS             6665..7177
                     /locus_tag="ACHGT5_000953"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7216957.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7433..7981)
                     /locus_tag="ACHGT5_000954"
     CDS             complement(7433..7981)
                     /locus_tag="ACHGT5_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN7216958.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8070..9251)
                     /locus_tag="ACHGT5_000955"
     CDS             complement(8070..9251)
                     /locus_tag="ACHGT5_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN7216959.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9487..10995)
                     /locus_tag="ACHGT5_000956"
     CDS             complement(9487..10995)
                     /locus_tag="ACHGT5_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN7216960.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11154..11345
                     /locus_tag="ACHGT5_000957"
     CDS             11154..11345
                     /locus_tag="ACHGT5_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216961.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11548..12465
                     /locus_tag="ACHGT5_000958"
     CDS             11548..12465
                     /locus_tag="ACHGT5_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN7216962.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12458..13531
                     /locus_tag="ACHGT5_000959"
     CDS             12458..13531
                     /locus_tag="ACHGT5_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216963.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13679..14197
                     /locus_tag="ACHGT5_000960"
     CDS             13679..14197
                     /locus_tag="ACHGT5_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7216964.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14178..14570
                     /locus_tag="ACHGT5_000961"
     CDS             14178..14570
                     /locus_tag="ACHGT5_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN7216965.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14646..15581
                     /locus_tag="ACHGT5_000962"
     CDS             14646..15581
                     /locus_tag="ACHGT5_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN7216966.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15714..16493
                     /locus_tag="ACHGT5_000963"
     CDS             15714..16493
                     /locus_tag="ACHGT5_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN7216967.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16486..16803
                     /locus_tag="ACHGT5_000964"
     CDS             16486..16803
                     /locus_tag="ACHGT5_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN7216968.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17047..18657
                     /locus_tag="ACHGT5_000965"
     CDS             17047..18657
                     /locus_tag="ACHGT5_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN7216969.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18814..19662
                     /locus_tag="ACHGT5_000966"
     CDS             18814..19662
                     /locus_tag="ACHGT5_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN7216970.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19893..20372
                     /locus_tag="ACHGT5_000967"
     CDS             19893..20372
                     /locus_tag="ACHGT5_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN7216971.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20405..22633
                     /locus_tag="ACHGT5_000968"
     CDS             20405..22633
                     /locus_tag="ACHGT5_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7216972.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22762..24405
                     /locus_tag="ACHGT5_000969"
     CDS             22762..24405
                     /locus_tag="ACHGT5_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN7216973.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24478..25023)
                     /locus_tag="ACHGT5_000970"
     CDS             complement(24478..25023)
                     /locus_tag="ACHGT5_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN7216974.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25046..25531)
                     /locus_tag="ACHGT5_000971"
     CDS             complement(25046..25531)
                     /locus_tag="ACHGT5_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN7216975.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25521..26333)
                     /locus_tag="ACHGT5_000972"
     CDS             complement(25521..26333)
                     /locus_tag="ACHGT5_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN7216976.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26472..>27582)
                     /locus_tag="ACHGT5_000973"
     CDS             complement(26472..>27582)
                     /locus_tag="ACHGT5_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN7216977.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9035 a         5025 c         5521 g         8001 t
ORIGIN      
        1 acagaaaatt ttttcttcgt tatacttgta atagaatact attttataac aaaggggatc
       61 gaatcaatga aaaaaactgt tatttatact agtttatttg cagctttgtt tttagctgct
      121 tgtcaatcaa ataccaatca aggcacatct gggaatacgt cttccagtag cgaacagcgc
      181 tcttcacagg tatcggactc ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa
      241 agttctagtg aagcaagtac tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa
      301 ttgttccaag ataaataccc agatactgca attactagtt tggaattaga tagtgattgg
      361 ggatcttact tctacaagat agaaggtgtc gatgatcaaa aagaatacga agcaagaatt
      421 gatgctcaaa atgaaaaaat ggagacagag aatccagaaa ctctagatcg agatgaacaa
      481 aatggtcaaa agaaaaaaga agatggtctg gatgttgata aattgatcac gattgaggaa
      541 gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg caacagattg ggatttggat
      601 aaagatttag gtactactta ttgggaagtc aaagtaaaag acggcaatca aagtacatca
      661 gtcaagatca atagccaaac aggcgaagta ctgtcaactg aacaagatga ttaagaattt
      721 cttcggaaaa caagagttga tttttctttt agtcatgata cactagacaa aataacttgt
      781 ttgaaggagc gatttacttg agtttagaaa cgacctttgc caatcatatc tttgccaatc
      841 ctttgatgaa tgcatctggt gtccactgca tgactaccca ggaattagat gaactggctc
      901 attcagaagc aggagcattt atcactaaga gctgtacgat caatgaacga aaaggaaatc
      961 ccgaaccacg ctattttgat gttcctttag ggagtatcaa ttctatggga cttccaaact
     1021 taggtttttc ttattatcta gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa
     1081 atcaaccgtt atttttttcc attgccggga tgagtgtcca ggaaaacttg gaaatgcttg
     1141 agaaaataga aaaaagtggg tttaatggga tcactgaatt aaatttatct tgtcccaatg
     1201 tgcctggaaa accacaacta gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag
     1261 tattcagcat cttttctaag ccgctaggaa tcaaattgcc tccttatttt gattttgctc
     1321 attttgatca gatggctgat atcttgaatc agtttccatt gacctatgtc aatgctatca
     1381 acagtgtggg caatggtttg tatatcgata cagaacaaga agcagtagtc atcaaaccaa
     1441 aagaagggtt cggcggcatt ggcggagagt atatcaagcc gactgctttg gcgaatgttc
     1501 gcgcttttta tacacgtttg aaaccagaaa tccaaatcat tggtacaggt gggattcgga
     1561 caggacaaga tgcatttgaa catctgctat gtggcgcaag catgttacag attggtaccg
     1621 agcttcataa agaagggccg gaaatttttt ctagaatcat aaaagaatta acgcagatta
     1681 tgtcagaaaa aggatatacg tcaattgacg aatttaaagg caaattacgc accatttcat
     1741 agcaaagaga cgaaggtcat gattgatctt cgtctttttt tataaaaaac actcgtctgt
     1801 tcattcctgt gcaagcgtac tcattgtatg ttagacgaac ttgtgggaaa ataacagagg
     1861 caaaaaggag gataatatga atgatgcaac agaattagca aaagagctta aagaaaaaga
     1921 atttagtttt caagaactag ttacggatat acagaaaaaa ataaagaagc aaaatcatga
     1981 attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat tcccacgagc tattgattcc
     2041 ccagtcagaa aaagggccat tggctggtat cccatttcct ttaaaaatgc tcggtcaaga
     2101 aaaaaaggga tggctagcta cttcaggatc tcgtttattt gaaacacacc gagcttctca
     2161 tacatctaat tatgtgcggc aagcagaagc gataggtcta gttccttttg gacaaaccaa
     2221 tgcgccagag tttggtttca aaaatattac agacccagtc atctatggcc cagcaagaaa
     2281 cccttggaac cttgaatgta caccgggcgg atcaagtgga ggagctgctg ctgcagtttc
     2341 atcaggaatc gttcctttag ctggtgctag tgatggcggt ggttctatta gaatacccgc
     2401 ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg acgatgccag ttggtccata
     2461 tgcttggaga ggatggcaag gagctgctat tgatttcggc ttgacagttt ctatgagaga
     2521 cacggaggct ttattttatg ggatgagaag tatccattcc ggtgcgccat atcaagtatc
     2581 gccagttgag tggcaaacac atccaagaaa aaaacggttg aagatagctt tttgtgttga
     2641 ttcaccgatc cactcacaga ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga
     2701 tttccttgga aaagaagggc atgatttgac agagattcct tacccactag atggtcgtaa
     2761 actgatcacg tcttattatc agatgaatgg cgcagaaaca gcagcaatga tccgctcgat
     2821 tgaaacaggc atccagcgag ctgtcagaaa agaagaactt gaacccattt cttggacttt
     2881 gttccaatat ggtcagaagc tctctgccgc tacttacatc gattctctgc atgtatggga
     2941 tgaagcagca attgtcatgg aaaacctaca tcaagaatac gatctattcc tttcaccaac
     3001 gactgcattt accgccccta aaatcagcga agatttgcaa agtgatgcga taagagcaca
     3061 gatggaacac atcagtgatt gttctgaagt agaaggttta gacgttgttt cttccatgtt
     3121 tgaggagagc ttgaagatca caccttatac acagttggct aatctgactg gacaaccagc
     3181 aatcagcttg cctactcatt tgacaaaaga aaacatgcct ctaggaatcc aatttatggc
     3241 tgccagagga agagaagatt tgctatttca aatcggctat ctatttgaag caaatcatca
     3301 gttccattta ccaaatccat ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa
     3361 agattgtttg ctctaataac tagtgccttt tttattttaa gcgcttgcgg tacagctgcc
     3421 gctaatcagg aagataacag ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg
     3481 acttctggca cactgtttcc cgcatcttat tataatgatc aaaatgaatt agttggttat
     3541 gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa
     3601 tacaacgtgg atggacaaat cacttctgtt tcaaaaagag aatcagattt tgctgcgaac
     3661 gattttggta tcacaaaaga aagagaaaaa aaattctccc tttcagagac tttcaaatat
     3721 tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag gaatccactc acttgaagat
     3781 cttaaaggga aaaaagcagc tggcgaaccc aatactagct atatgcgttt agcggaaaaa
     3841 cacggagcta aattagtcac atatgacaat gccacgaatg atcagtattt aagcgatgta
     3901 gcaaatggtc gaacagatgt tattctaaat gattattatc tgcagaaaat ggccgtatct
     3961 gctttaccag atatccctgt aaaaatactg gagaacgttt atttcaatga aaacgaaagt
     4021 ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa
     4081 gagatgaaag aagatgggac gacaaagaaa atatctgaaa aatattttca aactgatgtt
     4141 tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg agtgatgtac tttcctctaa
     4201 ttgatttttc cctcatgtgg acttcacttc ctttcgtact gcaaggcttg gtatacacat
     4261 tagggattgg cttcgtgtcg tttcttttag gaaatctggt agggttgctt ttgacagttt
     4321 taggtctttt ggattggctg cctctgaatg tatttattcg tttttattta tcgttcttta
     4381 gaggcattcc cgcactagtc ctgctgtttc tgttatattt tgggttacct tatcaattaa
     4441 gtgccttaac ggcttcagtc atctgcttta cgattaccag cagtgcattt attggtgaaa
     4501 tctatcgagg atcactagca ggcgtttctt caggtcagtg ggacgctggt ttagcttctg
     4561 gtttttcttt ctttggggta ttgaaatata tcgttttgcc gcaagctttt cgaatctcag
     4621 taccggcact cagtaacgta gctatggatc tagtgaaagg tacttcgtta gcagcaacaa
     4681 tcacagtgcc agaaatattt caaaaagcaa agatcatagg cggtcggaca ttcgattatt
     4741 tttctctcta tattttagtt gcactgattt attgggtcat ttgtatcggg atcgaaaaag
     4801 tgcaacagta tttagaaaaa cgttctatgc atcagtcaat ctgaaaaata gaaatggtgc
     4861 tagcgtggct ggcaccattt ctattttttg tttcttgtat attctgccta gtattttaaa
     4921 accatttcat gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca tgaagacccc
     4981 gctaaccatc gaaatagaat cctcgttata ccaatattgc atcgagcaag gcggcttggt
     5041 tgtagaagaa gtcacgatgc ctgatgacca aggaattgta gatacgctta gctgcttgac
     5101 aaaatttgat ggaagccgag aatggcgatg ctatgagctg aaagtgacaa aagcagattt
     5161 tcgctctaca gccaaactgt catttgttgg acattataat tattttgttt taccaagtaa
     5221 actatatgaa gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc
     5281 gtacacggtt gaagaggaga tgcctgcctc tggtacattt ctgattgcga aaaaagcagc
     5341 aaagaaagag cttgctgtac cggaagaagc tttgacaaac cgcttcatgg cttctttatt
     5401 cagggaagta agaaaagcaa agcaaatgga gcgaggcgta aaatattttc caacagatca
     5461 attgtacaaa gaattgaaaa gacgttcaga aaatcaagat ccattttcta acgaacgata
     5521 ttatgaacgg tttttagagg aaatagctag tcaggcaatc aatgagttgc aagaagaagt
     5581 tatcgcgctt cagcatgatt atgaatattt gaggcaacaa cagcagatcc gtcggttgcc
     5641 tacagagcca ttggagtgaa aaatatgtac tatccttatt taagaggaaa acaatttgac
     5701 ctattagcat taaaagaagc actgtcgcga ggattgctct ccaataaaat ccaaccagtg
     5761 atcgaaccag tacgtgattc cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag
     5821 caccatccaa tcgctgtgat ccaaaatccc caagtaggac aattcaaatt attcgatcag
     5881 catgtgcaca gttggacagt caaagaaaat agctctgtcg tccctgcaca gatcttaacg
     5941 ccagaaaatc tcagtgaagt actggattca cctccagcgt ttcttgtttt cgatggacag
     6001 cactacccaa aagatacaga ggtgtggaaa cagttggcag gagttgattc gaaatttttg
     6061 ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac
     6121 tcttttcaaa cgagaaagca tgttgaaagt tatgcagata aaacagatga cttttttagt
     6181 gatgactatc tttttttcca cgcagacgga tatattggat tttctgattt cacgatcgaa
     6241 ggtagccgtt atttcgataa agggttccct agccgagcgc ttgctatcca tctaacgtat
     6301 attgatgctt acggcaatat acgcgtgaaa cattttgtct ctgacagtaa cgactctgct
     6361 aaagaccaag cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt
     6421 caccatcatc aactcttgat tacttcagga atgattgaat tattggctct ttaccagcaa
     6481 caaaaattcc ctggactggg agtgttgaaa aagtggtcac ttatgcatca tttggaactt
     6541 attagtcacc tgctagatca tccaaaagat tggcttaaat attattcaaa agttcaggaa
     6601 ctatatgaag tgatccaatg attagataat gtaaatgaaa aaggaataga aaagaggaaa
     6661 ctggatgtat attcggcaaa caaggaaaga agaagtagaa gaagtggtag aaatcattaa
     6721 tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc
     6781 aacaaaagaa acgattttga acgatattga aagaaaagaa agctatgtat taatagtgga
     6841 agatcaaatt gctggaacag ctgcattagt ctcaagcgtg gatcccgttt acacaaaaat
     6901 agatggtagt tggaaagaat caactggtcc ttatttatcg attcatcgtg tcgctttagc
     6961 agaaagtact cgaggaaaaa aactagcaaa accgttctta gaacatttgt tgacgattgc
     7021 agccagcaaa aatatccaag atatccgtat cgatacttat ccagctaatg aaccgatgga
     7081 aaagaccatc tattctgctg gattcactta tcaagggatg attgaatttc cttttgaaca
     7141 cggcgaaaga aaagcatatc aaataatcat caattgacta taaaatcatc tgagcaaaga
     7201 taaagacatt aacactactg aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa
     7261 gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa
     7321 ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac
     7381 atagcttttg tcacgacctc tcggcttatc ctacgagtat tattcgatac atttatatca
     7441 ataatccttt ttttatttta tctgcagctt cttggaacgc tccatcgttt gacacgacta
     7501 aaagtgaggg gatagctttc aaacgtttca actgtgctaa atcttctaca taaaccgcca
     7561 gtctggctga aatctgttca gatgaatctc cacgtttttt caggcgcgat tgcaatgttt
     7621 ctttagagat cgtaataaag ataggacgga ctaccgtccc gaaatactca tataaatgcc
     7681 aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc tttttaattt
     7741 ctgctttact ggaaccatat ttatgttgag catattggtc ccactctgca aactcatttc
     7801 ccttgatcat ctgattgaat gtattgtcag aaacaaaata ataatcctct tgatcttttt
     7861 ccccaggtct tggtgatcga ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag
     7921 cttctgcgag tgaagttttt ccagaaccag aaggaccgat aaatacatag caataattca
     7981 ttgtcatttc tcctttgatg attgaacaag tgaaaaagct ttggaaaata tttccaaagc
     8041 ttttaaaagt aactttgctt ttttgtatat tataccagca agccagtcac aatagcactt
     8101 aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc gacaagattt
     8161 cctttttctt catttaatcc cttcatcgca ccagaaatga tcccgataga agagaagttg
     8221 gcaaatgaaa cgaggaagac agatataatt gctgttgtac gctcagtaaa atggaaatta
     8281 ccatttgcta attctgtcat agcaacaaat tcatttgtaa ccagtttagt cgccatgata
     8341 ctaccggcat ccactgcttc tttccatggg attccgctga tgaaggcaag aggggcaaag
     8401 atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat agcattgatc
     8461 atggcaatca aagcaacaaa accgataagc atagcagcaa ccgtgatcgc cacatggaaa
     8521 ccatcaagaa tgtattctcc caacatttgg aaaaatgtct gttctttgtt ttcttcaatg
     8581 atcagctcat cttctttttc ttctaataca tagggattta caattgaagc cagaatataa
     8641 ccgccaaata agttcaaaac aagggcagtg atcacatatt tagaatcgat cagtgccata
     8701 taagatccga cgatcgacat agaaactgta gacatagccg atacactcat ggtgaacaaa
     8761 cgtttctcag ataggaaggg taattccttt tttatggaaa taaaaacttc cgattgtcct
     8821 aaaatagcag aagctactcc attataagat tccaatttgc ccaacccgtt gattttactt
     8881 aatcctagac caacagcttt cataaataat ggaagaatct tgatatagcg tagaataccg
     8941 ataatagcag aaataaatac aattggcatc aatacggaaa gaaagaaagg tgcacttcct
     9001 ttatttgcta caccggcaaa aacaaagtca acaccttgcc cagcaaaagc aagaagatga
     9061 tcaaacattt ttgccaatac agaaacaacc gctgtaccaa aagttgtacg taataatacg
     9121 aaaccaagaa caagctgtaa aacgaacatc acaattagtg gtttcaattt gattgctttt
     9181 ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat aatacctaat
     9241 aaataactca aataatcact cctattccaa attccgtttt attttacctg ttttgcaaat
     9301 aaaatactaa tctttagtga aattttcatt acttttttag agtgaaacgc attcattttc
     9361 cattagaaaa taaagcgaac aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc
     9421 tataaaaaaa cgaaactatc gattaagcta atagtttcgt tttcaacatt tataagtaaa
     9481 ctttttctat tggtaatata gattttcgga agggtagaca ggtgcgcagg taatatcgat
     9541 tcctagtttt ttcaatgttt gttcatcatt tttattgacc atcacagtag agtgggcttg
     9601 tgctccctca agttcagcta gtttatcata agcaacttga gcgattggat tcgttactgc
     9661 actgattgat aaagcgatca agacttcatt gatgcttagt gcatccaaag aggaatgaag
     9721 tgtctttgat ttcaatcctt gtattgtttc aagaactaat ggtgacaata aaggaatgtc
     9781 atctgcgata tgggctaaat gttttactgc atttaagata actgccgctg acgcatccat
     9841 caatgaactt gtacgcccgg taatgatgac ttcatctggt aattcgattg caatcacagc
     9901 agaaggatct tttgtaggac tttgcgcttg aatcttatct gcatagtcat gggcagggac
     9961 taccacgttt cggtcttcag gacgtaaacc gacttcttcc ataatcagtt tgattcgatt
    10021 aactgcttct tcgtcgatca atcctttttt gaagtcgcat tctgtttgga aataacgacg
    10081 gatgatttct tgtttagagg cttcttggac aacggtatca tccacgatcc caaatcctac
    10141 acgattcaca cccatgtcag taggagattg ataaacagat tctttaccag taattttttc
    10201 aataatacgt ttgattacag gaaatgtctc aacatcccga ttatagttca cagctacttt
    10261 attgtaggca tcaaagtgga aggaatcgat catattgaca tcttttagat cgactgtggc
    10321 tgcttcataa gcaatgttca gcggatgttt caacggaaca ttccatactg ggaaagtttc
    10381 gaacttggag taaccagctg catgtccttt ttggctttca tgatacagtt ggttcaagga
    10441 agtagcgagc tttccacttc ctgggccagg agctgtcact acaacgattg gtttagttgt
    10501 ttcaatatac tcattttttc caaaaccatc ttctgagacg attttttcta cgttgactgg
    10561 atagtcttcg atttctgagt gtttatagac cttgatgttt cgtctttcta gtttattgat
    10621 aaagactttc gtggcaggct gtccactata gcgagtgata acgacactgt tgattaataa
    10681 gccataacca cgtaattcat cgattaaccg caagatatcc atatcataag taatcccata
    10741 atcaccacgg atcttattac gttcaatatc tccagcatag acgcaaatca gtatttcagc
    10801 ttgatctttc aatttttgca gcaatttgat tttcgcatct tcttcaaatc ctggaagtac
    10861 acgtttcgca tgcttgtcgt caactagctt cccaccaaat tctaaatata atttgtcata
    10921 gtggttcact cgttccaaaa tatacgctga ctgctcttca atatattttt ctgaatcaaa
    10981 accaattttt ttcactaggt tccctcctta agtactaaag cattttttca tttttttgag
    11041 gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac
    11101 ttgtctagat agttattcat tggtatgata gtttttgata aggaaggtgc tacatgaaaa
    11161 aaagacagaa aaagaaaaac gcatataaac actatatccg ttcgattttt accggttatg
    11221 aaagaatgct tgaagatccc gaattggaac aattgacgtt tacttatcta aacgaagaaa
    11281 cgcagctaac tcgtgatgag catcagcgca ttcatttcac aacaagagat ctgcctagta
    11341 aataaaaaat aagataaagg tcatcggatt cgattctgtc ctatcagatg gaaagactag
    11401 ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct gttttatgga gcattgcccg
    11461 ctctttttta taacaatttt ttacagaaaa tggtactatt gaagtattaa taagtgaaaa
    11521 acagatgagt aagaggaggc aagtagtatg aaagagccag atacaaatgc aaattatgag
    11581 ccagacgaac tagaggagac gaatgcggat caggatacag gacaagaatc gtcttctttt
    11641 tacgatatca aagaaaaagc aagcatgaat ctttcccaat tattagaaga cttacaggct
    11701 tttgaaaaag ctgtccgcga agaagatatc cctgagatct accgtttata caatggccga
    11761 ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg agatcgatca attgctgatg
    11821 cgtaagatcc atgatcgttt tttacaggaa tttcctttta tggaacaagt agaaaacatc
    11881 tcgccaacga tgagctatta caaaatcggc acttattacc atgatcggcc cacggtcggt
    11941 attgatgcaa gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa
    12001 tattcccaac cggagtcaac ggactatgac aaaaaaatca atgagctaga cgcaaaggtg
    12061 atcactgctc agacagaaat tgaacgttta gatgaacaaa taaaagaaat caatcgacaa
    12121 atgactgatt tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt
    12181 caagaattag agaagaagaa gcaagtacta tctaatgaaa aattaggctg gcttccttat
    12241 attgagacgc ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga agcaagagca
    12301 gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt
    12361 agcaaagagg gattcggtca agcaatccat gaattcttga tgaattatct tggcaatgag
    12421 aatcctaata atcaatctaa tgaaggaggt tccgagtatg aatgaacacg atgaagcaga
    12481 atggacaaaa aatgaagaac aaacagaaga tcatgaagaa acgaacactt cattggaaga
    12541 acaaacaagt aatcctctgg atgacacaga agaaatccct atgctagagg aaacaaaaat
    12601 acacgaaaaa gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca cccatgctta
    12661 cgaaataact gaaaatcccg ggtatttaga aggaaaagag caagatcatc aagatatcgt
    12721 cagtttgatt gaaacagcgg aagcagaatt agcggattta cgtatgagaa aagatttgat
    12781 cgaatcagat tttccacaat tattagaatc atatgatcgt tttatattga atgaagaacc
    12841 aatctccacg atcggaaaga caagcctgat cgaatacttc acgtatgaat tactgaaacc
    12901 gttgaatgat attggtctgg aacggtcaaa ttcagcttat gacacatgga aaacaagaca
    12961 tttttcgatc aattatacgt tgaatggaca aaacgaatta gtcttttcct ttgtattacc
    13021 gaccatcgat caacgatacc aagtggaatc gatgagtctc ctaaacgtat caccagagac
    13081 aatggaaatc aatgtccaag atgaccgggt gctttctttg atccgacact ggtcagtgga
    13141 tcgagtattc tctgcagggc agatcacgat attcaatcac aaattgaatc aattattagc
    13201 ccatgcaaga acacttggtt ttaccgttaa tcagacatta ctagacaata cgaagccact
    13261 tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa gtattggatg atatttttat
    13321 cgtggcaatg aaaaatccat cctacgatat ggaaaagatc aacgaaacta cttatcaagt
    13381 acgattagat aaaggacagt ctttaacgat aacaacaaac gaaaaagatc aaacagtctt
    13441 gtcgatctca tctggagact acccacgatc tgtcattgat ttctttatca attacgaatt
    13501 cttggtccca ttaatggtga gaaagtcctg attggataag ttggattacc agatgtcatt
    13561 cagataattc tgttccaaag gacttgcggc agttatccag aataattgtc acaagtcttt
    13621 tctgtatgta ctaaatggac gtgaataact tgctgatgaa ggagggattt cattaggaat
    13681 gtccacaaat aaaatagcac agcttagctt attgtctgct gcttgtgtag caggtaggat
    13741 cgcttttcaa tttattccga atttccagcc aatgactgct atttttttat ttatagtact
    13801 ttacttaagc ttaaaagatg cgttaattgt catgagtctt tctattgcga tcagcagttt
    13861 ttattttggt gttggaccat gggtcatcgg ccaatggatc agctatactg ttgttctgag
    13921 tttgttttca atcgtctgct tgagaaaaac agtgcaaagg aatctatggt taaagggagt
    13981 ctgttttttt ctggcgggca tggtttacgg aataggtatg accgtttttg atacgctgtt
    14041 atatcgtttg cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg atttgatgca
    14101 cagtataggc aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa
    14161 tcattcagga ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg agtgtcagta
    14221 tactcatttt gacaagttgc agtacaacct atacacaatc ggcttccaac caaaaggagg
    14281 aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa aacttttcgg
    14341 aaaaagaagt gttcttcaaa aaaggcgatg atttactgag tattttgaaa cgaaattttg
    14401 aagtcaaaga agataacggc ttcatcactt cgcttgaagg gcactcacag gatgagaaaa
    14461 atcaactcta ttggatgttt actgtggacg ggaaaagtgc tactactggt gccaaagaga
    14521 tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc taaactataa gtttcataaa
    14581 aaactgttaa agggcgaata ttgccataat tcagtgagtt cagttgatga aagaaggaaa
    14641 ataacatgga aaattttaaa aaaagcggaa tgttttccgt aattgctgct ctaggggcta
    14701 atattctagt tgctatcagc aaatttgtcg gctatgcaat cagtggcagt gccgccatgc
    14761 taaacgaaag catccacagt atcgttgatt gcagcaatca gattttgctg ttattcggtg
    14821 ataaacaagc caataaagga cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat
    14881 actttttcag tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca cttggggtga
    14941 tggaagcttt tgaaaagttg acgcatccat cccatgaagt gggtaatccg atcattgtta
    15001 tagcaatact gttatttggt ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag
    15061 aaatcaaaga attgaatact gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac
    15121 acagtgaaat actgattatt tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg
    15181 ctttagcagg aacaatattg acttatgcaa ccgggattgc tgcatttgat gcgatcagcg
    15241 gattgctgat cggtttcttg ctgatgggag ccgccatttt cctagcaaaa gaattttata
    15301 gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg
    15361 aacgtcctga gatcagtcgg ctgatcgata taaaaacagt tcatttaagt ccaacggaga
    15421 ttttagttgc agcaaaagca gatatcgttg gtaacaaaga gaatgaagct tactcggtca
    15481 tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg tatatataca
    15541 ttgaaacaga taaatatgac ccaaattatt ctcgtaaata aaacgtttat tcatgaattg
    15601 aagcaaaaat gagaaaaata taatcttgtt tttaaataaa acggctacaa tttagcgaat
    15661 ttatgttaca atgagtccgc tgtttatttg ttgtaattaa aggagatatt ttaatgaaca
    15721 aaaagaaaag tgcgccatgg cttcaagctt tgcatgcagt gatgccactt tgtataagct
    15781 atataccagt aggattagct tgcggagtat tgttgcaaaa agttggattt gatcctctct
    15841 tatcaggact gttatcgatt ctggtctttt ccggtggcgc gcagttttta gttgcatcta
    15901 tgcttacgac gcaagcatca tttgcaacga cattattgat ggttttcttt ttagaattac
    15961 gctatacatt attaggagca agtcttgccg gttttatgaa aaaagaaaag cggacgtttt
    16021 tggctgtctt ttctcaatca ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt
    16081 caactgaccc ttcctggaat aaaagaaagg ctttatatgt caattggttc tcaatgactg
    16141 catgggccgg tagcaatatg atcggtaatt ttttcggctc ggtgattcga gtagatgctg
    16201 accttgtcca ttttgctttg acggccatgt tcattttcat gtttatcatg cagatgaaaa
    16261 gcgcactact gatctttaca ggtttattct caggcgtatt aggggtcgta ttcatggtat
    16321 tattccaaaa tactttcgga ttgattgcag caacagtcat tgcttcattt gccggattta
    16381 tgctggagaa ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg
    16441 tcaatgagcc gctcttcaac ttcccggaac aggaggccaa tcaccatgac taaactatat
    16501 ttgctacttt tagttgcttg tctgttcatc gtggcatata tcccgcgctt gttcccgatg
    16561 ctttatttta ctcatcgaaa agtgccatcc tggttcagtg actggatgaa atatgtaccg
    16621 gtggcattat ttgcggcgtt agcttttaaa gatgttttca ttactcatga acatctggat
    16681 attgcttgga atatcaaaat agctgcaatg attctagtag caggcgtggc ttacaaaaca
    16741 cgttcgatgg cgttgtcagt tttgacagga cttgcgtctg tctttttgct gtctatgctt
    16801 taaactgttt tattttatta aaaacagaga ttgcgacata agttgttcaa actgatctat
    16861 ccgaactgca tttgcaacag tcaattctgg atgcaagttt attcgtaggg ataaaaaaga
    16921 ttagtgttgg cttatgatgc aagctctttt ttattttagg aattagagag ggatttgtcc
    16981 ttctatgatt ttttgctata atagggaaga tgtttatttt atgaaagaat agttgaggga
    17041 atttaaatga ataaaaaatt gatgcaagga acattttggc taacctttgc taatcttcta
    17101 tgtaaagttt taggggtagt ctatttgatt ccctggctaa gtatgatggg gaacaatcaa
    17161 gatggtatgc ttgctacgac attgtataat gttggctatc ttccttatgg attgttcctg
    17221 atgcttggta cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa
    17281 gaaggagata acgctgcttg tcggctgatt tttagaagta cgatcaatat catgtttgtc
    17341 atcggtatcg cctctgctgc actgatgtat ttatttgctc ctttactagc cggaatcagt
    17401 ccgattgcta acgttaataa tgggatacta gctatcagaa gtttatgccc atctttgatt
    17461 gctatcccaa ttcttagtgc aatgagagga tatttccaag gaaaaaatga tctgcggcct
    17521 tatggaactt ctttgattat cgaacaggct gtgcgagtga tcgttatctt ggctggtact
    17581 tattacttgc gcgtattgac tgacggaacc atattagaag ctgtattgat cagtacagtc
    17641 gcttctttct tcggtgggtt agctgcgatt atccatatgt atatcgtcgg acgcagaaaa
    17701 gacttcttcg aacttaaaga ttttttgatt tccagccgtt atttcgaaag agaaaaccga
    17761 tctgcctctg tctctatcat tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt
    17821 actatcgttc aaatgatcga ccaagtaacg atgaaaccac tgcttcactt tttccgtcca
    17881 gagatcgcag atcagcaact ggaattctta ttcagccgtg cttctgtcaa tccaaataaa
    17941 ttgacattga tcctgatctc catggtagga acagtagcta taagcagctt gccaatatta
    18001 agcacgatga agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg
    18061 attgcgttat tgattcttct tccttcattg actggaatgt ctttattagc tggaccacta
    18121 tacacattgt tttttggcta tgatcctgaa agtgtcggat atttccaaat ggctttgcta
    18181 gcttctttgt ttttctcttt attcacgatc ctatctacga tgcttcaatc tttgaatcac
    18241 catcgttttg cgatccgatt aactgtagaa gcaattatcc tgaaagtcat cttccaagta
    18301 atcggattag gattagttgg cggatacggg atgagcttat caaatggctt agcattcggt
    18361 attgtctttg ttcgcggcta tcactttatg tgcaaagaat accgaatagc acctttagca
    18421 aagatcagca attttttctt aaaaactttc cgctccaccc tgattatgct tgcagtatgt
    18481 tttgccgttt tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg
    18541 atttattgtg tggctgtagg aagtattggc ggcttagtat ttttattttc tcaattcgga
    18601 aaaaatgggt ttcgaatgtt gaaaaatgtt aaacatcata gcagaagcaa agaataggtt
    18661 aactaatcgt tttttgctgt cacttgaaaa acggcaactc gtatgattac gcttgtcgtt
    18721 tttctatttt ccaattttag tttcatatct aatcttactt atgatatagt tataaggtct
    18781 ggaaaaattc tttgaaacga ggaaattaaa cacatgaaac ttgcagtagt tacagatagt
    18841 tcggcatatc tgccggaacg tatcaagaac catcccgact tattcgttat tccaatccct
    18901 ttgattatgg atgggaaaat ttataacgaa ggaatcgata tcgaagcgga tgaatattat
    18961 ggacttttaa aaaacagtaa agattttcct actacttcac aacctgtcgt aggggaagtt
    19021 ttgggtttat atgaggaatt gaaagagaaa gggtatgaca cgattttaag tatccatctt
    19081 tcttcaggga tttctggctt tgtcaacaca ttatttgcga tgaaagatga catcagcgga
    19141 gtaacggtca ttccttatga ctctaagatc actagtatgc cgatgggaca catggtagaa
    19201 gctgccttag acttaaatga tgaaggtcag tcccttgatg aaatacttgc acatatcgat
    19261 cgaatccgag ataataccta cgcttatctg attgtagatg atttgaacaa tttagtacga
    19321 ggaggacgtt tgacaaatgg tgcagcgctg attggcagct tattaaaaat caaaccgatc
    19381 ctgacatttt ctgaagggaa aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca
    19441 tttgctcgag ctgaagaaat tattggtaga agaaataacg aaatacaacc gccagtcaaa
    19501 ctttacgtga tacatgcaaa taatttagaa gtagcgattg cggagaaaga gaaattgcag
    19561 aaaaaatatc caaatgcaga aatcgaaatt ggtcatttcg gaccagtcat tggtacacat
    19621 ttaggtgaaa aagccatcgg cattgccatt tcagcgcaat aattcctttt attcaaaaaa
    19681 cataccaaaa atattccaac atcatcttgg actttctaca tgttactaat gagcgtgtaa
    19741 aagaacgaga tggtgttttt atttctatca atgaagctct ttttctttgc aatcctcact
    19801 tcaatcctct ataatgtcgc atatagttta gtaaatgagt aaactactaa tttacttatt
    19861 tactaaaaat aaaaggatga ggagttaaaa taatggaaat accaacaaat ttaaaacata
    19921 aaccagtaat cgttgtagaa gactatgata aagttgatgg aagaaatgca ttaaatacgg
    19981 atgcaaaagg gctttcactg ggccttgctc agtggaatga tcgtggaaaa gtcgatatct
    20041 caggcaaggt atggcgacat actggagaaa aatggtcacg acagtcggaa gaattaccaa
    20101 tcacacgtat tttagattta gcgatcttag ctgcacaagg aagtttatac ttccaagatg
    20161 cttatcgtta tgaaaaattt tatgatccag aaaatccagt cgtagacatc attggattac
    20221 aagggaaccg aatgacggta gaagtagata ccaaaaatcc aaagatcgat gaagacatca
    20281 ttttgtatta cgatacacta caaaaagacg gagaactgat tggggaacga ttccgtattt
    20341 taaaacgtct tttagatgag ctaggatatt aatagacaga gatgatcaaa aggggaagag
    20401 aagcatggag aatttctcac taacagaaaa ccgaaccttt tcacggctag aaaagcaaat
    20461 gatctggaag aaaccgaaaa gccatcggac aagtgcagaa gaatggcgga tcgcctccga
    20521 aatcaaaggg aattggcaag atcctgaaat gaaaatcagt aacgtacttt tggaaggaga
    20581 tgccgggtcg gggaagacac aattagccaa agcactatct gctgatctgc aacttcccta
    20641 tacaaaagtg acctgttttg cagatatgga caaatcagat gttttcggag ccttattacc
    20701 tgtagtagat tctaataagg aacaagacca agaattacta gaagctattt accaaacaga
    20761 tacacttgaa gctgtactga cactgatcca gaaccattac ggcattgatc ctttttctgc
    20821 taaagaaaaa ttagcagagc tagttcaaag gatcgaagct gattatccag atgcaatcca
    20881 ttacagctat tacccttctg aaatcgttcg agcgattgaa aaaggatatt tattagagat
    20941 tcaagagccg actgtcatcc gagatgcctc tgttcttgtg gcacttaact cagcgctcga
    21001 accaaatggg atgttgaatt taccgacagg aatcattcgt cgccatccag attgcgtcat
    21061 tgtgatcact acaaatcgaa attaccaagg aaatcgccca ttaaatgaat ctttaagaga
    21121 tcggatgcaa catgcggaaa aaatggatct tcctgcctta gaagtcatgg cagaacgtgc
    21181 gatggcgaaa actatgatcc aaaaacaaga acttctctta aagatggcag agattatccg
    21241 tttgttggat gaaactgcaa aagctaacgc catcaaaggt gtagcaggaa tgcgttctta
    21301 tttttattgg gcaaatacct ataagcaagg acaagattta ctccagtcga tttatccaaa
    21361 agttttgtat aaattatcca ctgattcaga tgaattggca attcttgaac aagctttgtc
    21421 tgacagtgga ttgctactgg aacttcagga catattgaga aaagaaaaat ggggtcatcc
    21481 tcaatctgag catacaaaag gaagaaccat tagcgccgaa gaagcaaatg aacgaaacat
    21541 cactgaaaat gctgaagaaa ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga
    21601 accaaatgag ggagaagaga aacaagaaga aacaatagaa aaaaacgaga ttcctcctca
    21661 aatgcaaaaa gaacgcagta gcgatatcga aggaaaaaat caaaaagaac aacaaacagg
    21721 agatgattct caagaaatgt ctgcttctga catggaagca catgataagg aaatcaagaa
    21781 gcaactgaat aaagaagcgc gacaaatcat gaaggaaacg attcatgaaa aagaaggtct
    21841 catcgttcac cgaccagctt tccaaggaaa aacgccggaa gcacgtgagc tacacatgca
    21901 agtaatgcca atagtagaat cacttagccg gcaaatttta gagttactgg ataatgaaca
    21961 aacagagacg taccaaaaag gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc
    22021 atatggtgat ttgagaaatt ttgacaaaaa gaacccgcca catgagcaac cttctttagc
    22081 ggtagctgta cggatcgatg aatctgggtc aatgatacgg gatgatcgga tcgaagcggc
    22141 aaaaaaagca gcgattgcca ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat
    22201 ttacggagat acagcagacc gttcgtcaag agaaaagact tctttgtttt cttataaaga
    22261 atttgaagac ggatttcaat ggttaaatga aaaattagtc actatgaaac caaggcaaaa
    22321 caatcgtgat ggtgctgcac ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac
    22381 aaccaaactg ttgcttaata ttagtgacgg tcagccaaaa gcgttgccgg attatacagg
    22441 caaaaaagca aaagaagata tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct
    22501 atttgtctcc gcagctattg gccaagacaa agaagaaatc aagtcgatct acggtgaaag
    22561 tcgttttgta gatattactg acttgaatga atttcccaag caaatgattc aactgattgc
    22621 ccgctatcta tgataaaata aagctcaacg aacgcactta gcagattcgt tgagcttttc
    22681 gtttctagtt accggtaaca aggatacttc taaaatagaa tcggttataa tcaagaggga
    22741 ccgttaaagg agtgaaacga tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc
    22801 tatcaaatct atcctgctag tttcaaagat agcaatggcg atggtcaagg agacatcaat
    22861 gggatccgtg aaaaacttaa ttatttgaaa gatcttggta tcggctttat ctggatcaat
    22921 ccgatctatc aatctccatt tgtcgataat ggttatgacg taagtgacta tcaagatata
    22981 gaagagagat tcggaacgat ggaagatttt gatttattgc taaaagaagc acatgaatta
    23041 gggatcaaag tgattatgga tcttgtcatc aaccatagtt cggatcagca tcaatggttt
    23101 gaagaatcga aaaaatctaa agaaagcccg tatcgcgatt attacatttg ggtcgacggg
    23161 gtagatggca aagaaccgaa taattggaca tcgattttcg gcggttctgc ttgggagtat
    23221 tctcatgaaa ctggacagta ttatctgcat gtttttgcaa aagaacaacc agacttaaat
    23281 tgggaaagcg agaaattgaa agaagaacta ttcaatatga tccgatggtg gttggataaa
    23341 gggatagatg gatttcgttt agatgcgatt tctcatgtaa aaaaagatga atattcagta
    23401 aaagccacag agaacccttt ctcacccttt caaaatgtct caggaatcga agaacatttg
    23461 accgatttga aacatgtttt tgaagagtac gatatcatga ctgtcggaga agcaagtggt
    23521 gtcactgcag aagaaggacc acaatgggta gggaaagacg gttatttcga tatgatattc
    23581 gaatttgatc atatccatat ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa
    23641 ttgaaacttg cattaagcgc ttggcaaact tcattggatg gtatcggatg gaacgcttta
    23701 tatatggaaa accatgatgt tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat
    23761 ttttgggcta tgtctgcaaa agcaatcgct atgatgtatt tctttttgca aggtacgcca
    23821 tttatttatc aaggacagga aatcggaatg acgaatatgc cctttgaatc gatcgatcaa
    23881 gttgatgcag tagattcgaa aagactttat aaaaggctgc ttgcggaagg taagacaaga
    23941 gaagaagcat tggatatcat tcgtgaaaca acgagagaca acagcaggac acccatgcaa
    24001 tggacttcgg aacaatatgc tggtttttct acacatgaac cttggctgat cactaatcca
    24061 aataccaaaa cgatcaatgt agaacaacaa gaatatgaac cagagtcggt cctgcaattc
    24121 tataagaaca tgatccgcat tagacaaacc aataaaggtc tgatatacgg ttcttataaa
    24181 gaatatcttc atgaacatcc acaactttat gtctatgaaa gatatttaga agatgaacaa
    24241 tatcttatta tggtaaactt aacagaatca cttgctgact atgaacttcc aaaagaagca
    24301 gatcagtcct ggacattact cttgtcaaat tcctctagcg gagaatttga agctaaaggc
    24361 atactagcac cttatgaagc tcggttatat aaaacaaaca agtaaaactt cctcgttaaa
    24421 gttcattagt tggactttgc gaggaagttt ttcagattgc tctgtcaatt actcatttca
    24481 ataattcgct ctaggaccac cgatatattt aggacgagag cgaagggctg tcacgtgagc
    24541 ttgtccaagg gttttcaaac gaggtctgac aggtacttgg acatgtttga tatgcatacc
    24601 gattgatgtg tcaccgatat cgataccggc ttgtccaact acgtgttcca cttcgacagg
    24661 atcattgaac tggtcaaaag cagcaacaga acaagcacct ccagcatgta gtgcaggaac
    24721 aacagagaca atttcgaaat tttttgcttc tgccactgtt cgttctacta ctaatgctcg
    24781 gttcaaatgt tcacaacctt ggacagctag cgcaatttct ttaggatcca acaactcttt
    24841 taacgtacgg atgatccatt gaccaacttc cgcactggaa ttttttccaa tatgtcctcc
    24901 aacgacttcg cttgtgctgc atcctaaaac aaaaagatca ccttttttca agtgtgcctc
    24961 agccaatatg tcattaacta tttcagtcaa ttggtctttt aattcttttt ctgacaactt
    25021 cattttctaa aaccttcttt ttcaattaat attgcttccg tgcttgaata cgttgaagac
    25081 ttgcagttat aggaaaggca agtcccattc caatcagatt ttggaagatg tttcctggta
    25141 tagaagccac tccagccggc caatcatata aaatccaacc ggcgatacca tagccgatga
    25201 tcataactaa gcttcctaat aaaagcccta atccccacat acgagtagaa gatttgctga
    25261 aagaaccagc aacatatcct tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg
    25321 gatatcctga aatcaaatcg atcaatagac cactggaagc accaacaact aatccacccg
    25381 ttggaccata aagaaaagca gcggtataga tcccggcctc gcataatgtc acaaatccat
    25441 tcgtagccgg tacagggatc aaaattgtta atgaaagggc aactgtcaac gcaatcagca
    25501 tagcatagat cgttaattgc ttagttttca tttctcattc ctcctttttg aataccgcca
    25561 gaccagacac ttccggtatg atcgttcatc agcacgccat tagaaataca atgggcaaca
    25621 aattttttac tttctgctac tgctttggta aaatctgccc ctaacacaag attcgctgtt
    25681 attgcagaag aaaaggcaca accggccccg tttactgttg tagtgtcgat ttttttatga
    25741 gaaaatacct cataatgatc aattgaatag agaagatcca cagcttcatc tccagccatc
    25801 cttgtgccgc ctttgatcac aatgtttttt gttcctgaag cagtgagttc tttagcgaga
    25861 tggatcattt ctgttaacga agagggaaga gcttgaccag aaagtaaagc agcttctgcc
    25921 aaattaggcg taatcatcgt aacaagtgga aaaagttctt cgatgagctg cgaaatatac
    25981 aattgattga tcacagcttc agtttctttg aatgccaaaa cagggtctaa aatgattggg
    26041 atattttgtt ttgtttttat gaagtctcga acaaccatga ttgcttcact tgaattcaat
    26101 aacccaattt tgataccatc taatgaagtc attttatcaa tcgaaactaa ctgtcgttta
    26161 agcaattcca aagatataga ttccagcata aatcgctcat taatatctag tgcccctata
    26221 gaagtcaagg cgcttagtcc gaatagacca aaattctcaa aagtttttaa atctgtttgg
    26281 attcctccac cagcaaatgg atcagaaccg ccaattgtca agattctttt cataacattt
    26341 tcctccatga ataagagtat aacgaaattt tcaaaaaaga aaaacagcca attggcatat
    26401 ttttatctca tccaatttaa aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt
    26461 tccttaaata gttaggactt ttgtgatttt aattctttct cgatttgaag ccgttgcact
    26521 ttcattgtag ctgttcgcgg gattgcatca aaatcacgga tgatagggac gttcaagcga
    26581 ggaagttcat ggacttgctc ccaccatcga ttccaatcca tttctttatc aggagctaaa
    26641 gcaataatcg gttgtggaga atgattttta tcccttacaa tcactacttc tgccaaaaaa
    26701 tctaacgaat ctaataaaaa gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa
    26761 tccacttgcc gatctttcaa aaataggtgg ccatgcttat ccataaaacc gtaatctccg
    26821 ctatcccacc aatcgccata aacattttcc tggaaccgtg catcttcctt gtaataagtc
    26881 aaagcccgtc ctttagacag taattgtata tgaccatctg tgttttcagg taggacattc
    26941 ccaactgaat ctgtaatccg agcgcttgtc atgtcttcca gtccaacgcc catatctctt
    27001 gcatcacttg ttttcaaact ctctagtgta tgagccttaa gaatcattgg tccgcattca
    27061 ctttggccat atacttgaag aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc
    27121 ttcattgtct ggttgtttat agcatcaaaa gtggagtgat agtatcggat acccgaaaaa
    27181 gcttctggat gctccttagc agtaaaacgc cactgaacaa agttattcgg atgggtctct
    27241 agtgcaattg gccggtgagc ttccaacata tgaacaactt ttgaactttg tgcgtttgct
    27301 aaaggcatca taggaaatcc catggacatc aaagaagaga taccaatatt gaatctggaa
    27361 tgcactggag agatgtgaaa agcgattaat ttcttttccg aaatttttgt gaaaactgtt
    27421 ttttgccatt tcgtccgcca tcccatagaa tgagcagaat gacaaataag tttagggata
    27481 ccagttgtac cagaagtatg ggtcatataa gaaatttcat ttttgtctaa aaatgattga
    27541 gaaacagaca aagcaggttg ttccattaaa taaagaatag ga
//
LOCUS       DAZBWR010000024        27568 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000024 DAZBWR010000000
VERSION     DAZBWR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.024"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..964)
                     /locus_tag="ACHGT5_000974"
     CDS             complement(20..964)
                     /locus_tag="ACHGT5_000974"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN7216978.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1260..2204)
                     /locus_tag="ACHGT5_000975"
     CDS             complement(1260..2204)
                     /locus_tag="ACHGT5_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN7216979.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2425..3639
                     /locus_tag="ACHGT5_000976"
     CDS             2425..3639
                     /locus_tag="ACHGT5_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7216980.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3661..5676
                     /locus_tag="ACHGT5_000977"
     CDS             3661..5676
                     /locus_tag="ACHGT5_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN7216981.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5759..7132)
                     /locus_tag="ACHGT5_000978"
     CDS             complement(5759..7132)
                     /locus_tag="ACHGT5_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN7216982.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7158..7442)
                     /locus_tag="ACHGT5_000979"
     CDS             complement(7158..7442)
                     /locus_tag="ACHGT5_000979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7216983.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7444..9459)
                     /locus_tag="ACHGT5_000980"
     CDS             complement(7444..9459)
                     /locus_tag="ACHGT5_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN7216984.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9638..9943)
                     /locus_tag="ACHGT5_000981"
     CDS             complement(9638..9943)
                     /locus_tag="ACHGT5_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN7216985.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10032..11063)
                     /locus_tag="ACHGT5_000982"
     CDS             complement(10032..11063)
                     /locus_tag="ACHGT5_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN7216986.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11270..12145
                     /locus_tag="ACHGT5_000983"
     CDS             11270..12145
                     /locus_tag="ACHGT5_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN7216987.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12297..13139)
                     /locus_tag="ACHGT5_000984"
     CDS             complement(12297..13139)
                     /locus_tag="ACHGT5_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN7216988.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13231..15108)
                     /locus_tag="ACHGT5_000985"
     CDS             complement(13231..15108)
                     /locus_tag="ACHGT5_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN7216989.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15105..16526)
                     /locus_tag="ACHGT5_000986"
     CDS             complement(15105..16526)
                     /locus_tag="ACHGT5_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN7216990.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16529..19918)
                     /locus_tag="ACHGT5_000987"
     CDS             complement(16529..19918)
                     /locus_tag="ACHGT5_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN7216991.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20175..21566
                     /locus_tag="ACHGT5_000988"
     CDS             20175..21566
                     /locus_tag="ACHGT5_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7216992.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21639..22286)
                     /locus_tag="ACHGT5_000989"
     CDS             complement(21639..22286)
                     /locus_tag="ACHGT5_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7216993.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22294..22980)
                     /locus_tag="ACHGT5_000990"
     CDS             complement(22294..22980)
                     /locus_tag="ACHGT5_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216994.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22986..24059)
                     /locus_tag="ACHGT5_000991"
     CDS             complement(22986..24059)
                     /locus_tag="ACHGT5_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7216995.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24281..24955
                     /locus_tag="ACHGT5_000992"
     CDS             24281..24955
                     /locus_tag="ACHGT5_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7216996.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24960..26300
                     /locus_tag="ACHGT5_000993"
     CDS             24960..26300
                     /locus_tag="ACHGT5_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN7216997.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26449..27435)
                     /locus_tag="ACHGT5_000994"
     CDS             complement(26449..27435)
                     /locus_tag="ACHGT5_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN7216998.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8033 a         5215 c         5011 g         9309 t
ORIGIN      
        1 agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa gcacttgttg
       61 atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact tcacgaacac
      121 cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg tattcaccag
      181 ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct tttacgatac
      241 gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg atctcataag
      301 cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata cgatggtctt
      361 tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat gcagcaactt
      421 ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca acttttaatt
      481 tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta ccgataacac
      541 gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct actgggtttg
      601 aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg atttggcgca
      661 tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt tggttgatac
      721 cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct gaccatacag
      781 ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc ccttctgctt
      841 tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg atcattgcat
      901 aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga cttgtttttt
      961 tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag tatagtcttg
     1021 tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt tgatggaaac
     1081 gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat cccctgataa
     1141 tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata
     1201 agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag attttcttct
     1261 taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa taatttgaat
     1321 aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga aaaactcgtt
     1381 tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa
     1441 gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc aaaatgagct
     1501 gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa tactctaagc
     1561 atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa taaatgagtc
     1621 aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg actgtatgta
     1681 tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt ttttccgccg
     1741 cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat caaaaaagaa
     1801 gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa ggtttcttct
     1861 tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct ttgagtagct
     1921 tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc attaataatt
     1981 tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt atgctcggga
     2041 acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa tagatcccct
     2101 cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt gatattcatt
     2161 ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc
     2221 agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta ttagttcacg
     2281 ctgtactgct attatatgca aagctatttt tggtttcata aagactttct atggttggga
     2341 catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat atttttgata
     2401 agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa attttctctg
     2461 ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat tcccagtatg
     2521 tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac tgctccctct
     2581 tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat cggacaaaaa
     2641 caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc cctcttctca
     2701 caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt tggattgatc
     2761 aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg agcctctatg
     2821 attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt tgttgcagga
     2881 cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag cttacctatt
     2941 cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcgcatggc
     3001 acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta tggtattttt
     3061 ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg
     3121 acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca
     3181 agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt
     3241 acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg
     3301 tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac
     3361 ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc
     3421 ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct
     3481 tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg
     3541 attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga
     3601 gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa
     3661 atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc
     3721 catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg
     3781 tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct
     3841 tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat
     3901 ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga
     3961 catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc
     4021 tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat
     4081 atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa
     4141 acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa
     4201 ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga gtccgatgta
     4261 gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa
     4321 gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa
     4381 cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct
     4441 tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg tagtgatttg
     4501 ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca
     4561 ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca
     4621 ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg
     4681 tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc
     4741 gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg
     4801 tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa
     4861 ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc
     4921 ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa
     4981 gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc
     5041 gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc
     5101 gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta
     5161 ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga acaagatgct
     5221 ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg
     5281 atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat
     5341 gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca
     5401 agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat
     5461 gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct
     5521 atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat
     5581 agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca
     5641 cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat
     5701 cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt
     5761 aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata
     5821 cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta
     5881 gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga
     5941 ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat
     6001 tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag
     6061 aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc
     6121 ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata
     6181 cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta
     6241 cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc
     6301 cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta
     6361 cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa
     6421 gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc
     6481 tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc
     6541 cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt
     6601 ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc
     6661 cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta
     6721 aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt
     6781 tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc atgatcaaag
     6841 cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta
     6901 ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga
     6961 ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga
     7021 tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga
     7081 ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt
     7141 cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc ttttacttct
     7201 tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta
     7261 tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca
     7321 tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg
     7381 atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc
     7441 atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa
     7501 taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca aagcccgaag
     7561 atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat gggctggatt
     7621 atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt cgtttacgcc
     7681 atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa acgcaccata
     7741 ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat tttctgataa
     7801 tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg
     7861 gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa
     7921 aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt
     7981 taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc
     8041 tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat
     8101 gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga
     8161 aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc
     8221 tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct
     8281 aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc
     8341 ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct
     8401 cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc
     8461 atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg
     8521 aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa acgaagcaaa
     8581 ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa
     8641 atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa
     8701 tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat
     8761 gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt
     8821 tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc
     8881 gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata
     8941 atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat
     9001 caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct
     9061 aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat
     9121 ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa
     9181 aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg
     9241 ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc
     9301 tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag gagttaattg
     9361 tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc
     9421 tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt
     9481 atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat
     9541 tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa agcaggtgac
     9601 caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca tgtttatttt
     9661 ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt
     9721 caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag
     9781 ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt
     9841 ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg
     9901 aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta
     9961 gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc
    10021 ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa
    10081 attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat
    10141 gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct
    10201 tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg
    10261 ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct
    10321 tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg
    10381 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    10441 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    10501 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    10561 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    10621 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    10681 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    10741 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    10801 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    10861 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    10921 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    10981 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    11041 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    11101 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    11161 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    11221 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    11281 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    11341 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    11401 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    11461 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    11521 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    11581 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    11641 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    11701 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    11761 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    11821 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    11881 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    11941 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    12001 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    12061 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    12121 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    12181 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    12241 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    12301 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    12361 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    12421 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    12481 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    12541 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    12601 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    12661 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    12721 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    12781 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    12841 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    12901 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    12961 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    13021 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    13081 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    13141 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    13201 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    13261 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    13321 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    13381 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    13441 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    13501 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    13561 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    13621 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    13681 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    13741 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    13801 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    13861 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    13921 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    13981 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    14041 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    14101 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    14161 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    14221 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    14281 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    14341 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    14401 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    14461 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    14521 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    14581 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    14641 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    14701 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    14761 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    14821 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    14881 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    14941 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    15001 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    15061 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    15121 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    15181 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    15241 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    15301 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    15361 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    15421 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    15481 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    15541 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    15601 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    15661 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    15721 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    15781 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    15841 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    15901 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    15961 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    16021 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    16081 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    16141 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    16201 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    16261 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    16321 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    16381 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    16441 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16501 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16561 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16621 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16681 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16741 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    16801 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    16861 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    16921 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    16981 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17041 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17101 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17161 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17221 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17281 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17341 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17401 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17461 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17521 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17581 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17641 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17701 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    17761 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    17821 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    17881 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    17941 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18001 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18061 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18121 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18181 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18241 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18301 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18361 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18421 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18481 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18541 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18601 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    18661 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18721 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    18781 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    18841 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    18901 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    18961 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19021 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19081 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19141 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19201 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19261 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19321 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19381 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19441 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19501 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19561 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19621 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19681 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19741 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    19801 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    19861 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    19921 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    19981 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20041 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20101 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    20161 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20221 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    20281 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    20341 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    20401 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    20461 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    20521 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    20581 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    20641 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    20701 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    20761 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    20821 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    20881 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    20941 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    21001 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    21061 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    21121 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    21181 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    21241 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    21301 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    21361 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    21421 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    21481 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    21541 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    21601 ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc
    21661 caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg
    21721 agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct
    21781 tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt
    21841 ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac
    21901 aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat
    21961 ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga
    22021 tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa
    22081 tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact
    22141 tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt
    22201 gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt
    22261 atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt
    22321 caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg
    22381 agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac
    22441 ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg
    22501 ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt
    22561 aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt
    22621 tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa
    22681 aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc
    22741 tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt
    22801 cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc
    22861 aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac
    22921 ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat
    22981 ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac
    23041 aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa
    23101 aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga
    23161 gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg
    23221 aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc
    23281 atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc
    23341 gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata
    23401 gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt
    23461 acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa
    23521 gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga
    23581 aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt
    23641 ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt tttaaattca
    23701 tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca
    23761 ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct
    23821 ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca
    23881 tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct
    23941 gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata
    24001 ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt
    24061 tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac
    24121 tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca
    24181 tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt
    24241 ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt
    24301 agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat
    24361 aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct
    24421 tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg
    24481 gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa
    24541 gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga
    24601 actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt
    24661 tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca
    24721 agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg
    24781 aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac
    24841 gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt
    24901 ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa
    24961 tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat
    25021 ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat
    25081 acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag
    25141 agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca
    25201 gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga
    25261 tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc
    25321 aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa
    25381 atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga
    25441 atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat
    25501 tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg
    25561 gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac
    25621 aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa
    25681 atagtttgga acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca
    25741 tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa
    25801 tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat
    25861 atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta
    25921 ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt
    25981 tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg
    26041 attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca
    26101 gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga
    26161 aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc
    26221 atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc
    26281 cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca
    26341 cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg
    26401 aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt
    26461 attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac
    26521 atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg
    26581 aataactgta tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc
    26641 atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat
    26701 gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt
    26761 gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt
    26821 tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt
    26881 acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa
    26941 tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt
    27001 gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat
    27061 tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc
    27121 agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc
    27181 ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc
    27241 atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct
    27301 agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg
    27361 caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa
    27421 atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt atattgttac
    27481 ttataaattt attctatagg agacgattgt tttctacaaa tatttagatt ggaatttgat
    27541 taaaacaatt taattgaaat ttgaaaac
//
LOCUS       DAZBWR010000025        27021 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000025 DAZBWR010000000
VERSION     DAZBWR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.025"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..1202
                     /locus_tag="ACHGT5_000995"
     CDS             198..1202
                     /locus_tag="ACHGT5_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7216999.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1203..2147
                     /locus_tag="ACHGT5_000996"
     CDS             1203..2147
                     /locus_tag="ACHGT5_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217000.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2147..3109
                     /gene="opp4B"
                     /locus_tag="ACHGT5_000997"
     CDS             2147..3109
                     /gene="opp4B"
                     /locus_tag="ACHGT5_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN7217001.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3136..4050
                     /locus_tag="ACHGT5_000998"
     CDS             3136..4050
                     /locus_tag="ACHGT5_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217002.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4076..5857
                     /locus_tag="ACHGT5_000999"
     CDS             4076..5857
                     /locus_tag="ACHGT5_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7217003.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6205..6891
                     /gene="rnc"
                     /locus_tag="ACHGT5_001000"
     CDS             6205..6891
                     /gene="rnc"
                     /locus_tag="ACHGT5_001000"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN7217004.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6911..10492
                     /gene="smc"
                     /locus_tag="ACHGT5_001001"
     CDS             6911..10492
                     /gene="smc"
                     /locus_tag="ACHGT5_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN7217005.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10501..11310
                     /locus_tag="ACHGT5_001002"
     CDS             10501..11310
                     /locus_tag="ACHGT5_001002"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7217006.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11321..12319
                     /gene="ftsY"
                     /locus_tag="ACHGT5_001003"
     CDS             11321..12319
                     /gene="ftsY"
                     /locus_tag="ACHGT5_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN7217007.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12506..14773
                     /gene="gshAB"
                     /locus_tag="ACHGT5_001004"
     CDS             12506..14773
                     /gene="gshAB"
                     /locus_tag="ACHGT5_001004"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN7217008.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14862..15314)
                     /locus_tag="ACHGT5_001005"
     CDS             complement(14862..15314)
                     /locus_tag="ACHGT5_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN7217009.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15464..16414
                     /locus_tag="ACHGT5_001006"
     CDS             15464..16414
                     /locus_tag="ACHGT5_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN7217010.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16407..17366
                     /locus_tag="ACHGT5_001007"
     CDS             16407..17366
                     /locus_tag="ACHGT5_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN7217011.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17363..18118
                     /locus_tag="ACHGT5_001008"
     CDS             17363..18118
                     /locus_tag="ACHGT5_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217012.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18141..19094
                     /locus_tag="ACHGT5_001009"
     CDS             18141..19094
                     /locus_tag="ACHGT5_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7217013.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19738..20034)
                     /locus_tag="ACHGT5_001010"
     CDS             complement(19738..20034)
                     /locus_tag="ACHGT5_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN7217014.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20389..20745
                     /locus_tag="ACHGT5_001011"
     CDS             20389..20745
                     /locus_tag="ACHGT5_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN7217015.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20755..21654
                     /locus_tag="ACHGT5_001012"
     CDS             20755..21654
                     /locus_tag="ACHGT5_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN7217016.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22174..22902
                     /locus_tag="ACHGT5_001013"
     CDS             22174..22902
                     /locus_tag="ACHGT5_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN7217017.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            23047..25074
                     /locus_tag="ACHGT5_001014"
     CDS             23047..25074
                     /locus_tag="ACHGT5_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN7217018.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25182..26279
                     /locus_tag="ACHGT5_001015"
     CDS             25182..26279
                     /locus_tag="ACHGT5_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN7217019.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26586..>27021
                     /locus_tag="ACHGT5_001016"
     CDS             26586..>27021
                     /locus_tag="ACHGT5_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217020.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT         9064 a         4676 c         5696 g         7585 t
ORIGIN      
        1 acacgtaaaa gtcatttttc tgataattct ggcgataaac agaaattatg ttgcaaagta
       61 aagcgatttt ctatataatt attaataatt cattacacaa agataagaat aacctttgtt
      121 ataaaacgct ttattctgtg taatgtcctt ttttattaag aaaaacttcc atttaggaag
      181 cagagagggg tattcgattg tccgataacc aattattaga agttcagaac ctacatacag
      241 gattccgact caaagatgac tattacgatg cagtagatga cgtttcattt actttagaca
      301 aaaacgagat tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc ttggctacta
      361 cgattatggg attacttgat cctaataaca caaaaattac aggtgagatt ttatataacg
      421 atctgaattt aatcgacctg aacgaaacgc tctataataa aatccgcgga aatgatatcg
      481 gaatgatttt ccaagatccg ttgtctgcat taaatcctct catgagaatc gaagaccaga
      541 tcaaagaagg actttcctat cacacaaaga tgaatgcaga acagcgtcaa gctcgtgcac
      601 tagaattgct agaacaagta gggattccta atcctgcgag agttggacgc caatatcccc
      661 atgaactatc aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc gcctgcaaac
      721 ctccaatcgt cattgcagat gaaccgacga cagccctaga tgtaaccatc caagcacaga
      781 ttcttgattt actgaaagac cttcaggaag aaacaaaaac agggatcatc ttgatcactc
      841 atgacttagg agtcgtagca gaaatggctg atcgagtggc agtgatgtat ggcggacagt
      901 tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc taagcatcct tatacacgct
      961 ctttactgaa ctctatacca caagaaggaa atcatgatgc agaactccat gtgatcgaag
     1021 gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca ccacgtattc
     1081 catggatacc agcaagcgat catgaagaag agcctatcct tcatgaagtc gcaccgaacc
     1141 acttggtacg ttgctcctgc tataagcatt tccactttag agacgaaaaa ggagaggtgt
     1201 agatgacaga attaatcaca atcaaagacc tgaaagttca ttaccctatt cgcagtggat
     1261 tcttcaatcg agtaacggat catgtatatg ctgtagatgg tgtggatttt attatcgaaa
     1321 aaggaaagac atatggttta gtgggagagt ctggctctgg aaaatctact acgggaaaag
     1381 ccgttgtggg attagaaaag gtaacagccg gacaaatcat gtatgaagga aaagatgtaa
     1441 cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg atcttccaag
     1501 attcaatgtc cagtttgaat cctaaaaagc gagtgttgga tatcattgct gaacccatcc
     1561 gaaattttga acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga ctgctagata
     1621 tcgttgggat gccggaagat gcgctatata aatatcctca tgaattttca ggcggacaac
     1681 gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc gtagccgatg
     1741 aacctgtatc tgctctggat ctatctgttc aagcgcaagt attgaatttc atgaaacgaa
     1801 tccagcaaga atacggcctc agctatttat tcatttcaca tgatttaggt gtagtaaaac
     1861 acatgtgcga caatatcgca attatgtaca aaggtcgatt tgtagaaatc ggtacaagag
     1921 aagacattta taatgacccg cgacatatct atacgaaacg tctgctttca gctattccgc
     1981 aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg acgaagagta gaacaagagt
     2041 atatccaaaa ccaaaaagaa tactatgatg cgactggtcg agtctacgat ttgcgtacga
     2101 tcacaactac acacaaagtg gcgttgaaag atggaggtgc aagctaatgt ggaaaacgat
     2161 tctgcgacgt gttttattga tgatcccgca agttattatt ttaagtgttc tgatcttctt
     2221 attagcccaa gcgatgccgg gggatccatt taccggtttg atcaatccaa atcaggatcc
     2281 ggcagtgatc gaacagatgc gacaagcagc tggcttgaat gacccatggt atgtccagta
     2341 cttccgttgg atcggaaatg ctttgcaagg cgattttggt caaagtttct tatacaaacg
     2401 ctctgttgct gacttgatcg gcgaacggat catcaacact ttatatctat cgatcttgac
     2461 agttgtcctt acttatttga ttgctgtgcc tctggggatg cttgctggcc ggtatcaaaa
     2521 ttcaatcttg gataagattg tcgtaatcta caacttcttt agttttgctg ttcctttatt
     2581 tatcttcggc ttgatcatgc tatttgtttt tggttatcgt ttaggctggt tcccaacaag
     2641 tggatcacaa acactaggtt tgcaaggtgg atttatcact caatggatag accgtttgaa
     2701 atatattctg ttaccatcaa ttacccaagc atttttggcg actgccgtca caatccaata
     2761 tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt gtcagaacag cccgttccaa
     2821 aggggtaccg acgaataaag tattcagtcg acatattttc cgaaatgcag cgttgccgat
     2881 ggcttctcaa atggggtatg aaatcacttc gttgattgct ggttctgtag ttatcgaaaa
     2941 gatttttggt tatccgggag tcggaaaact atttattgat tccatcggcc aacgggacta
     3001 taccgtgatc acatccttag tattgatttt aggaatcgcg acgctagtgg ggacattact
     3061 ttcagatatc atcatgagta tcgttgaccc gcgtattcgg attcaataaa accaatcaga
     3121 aaggagaatg gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag tattcctcct
     3181 atggggatac gaatgattgc tcgagaattt aaaaaagata aaattgctat tttttcatta
     3241 ggtttgctag tggttttatt attagtgatc tttatcggtg cgatgctgac cgatcaagac
     3301 aaagtgatgt atgttagtat cttcgacaaa tacgctgcac caggaacagt ttcgacacaa
     3361 ggacaaaagt tcattctggg agcagatgaa ggcggacgtg atgtattagg acaattgatc
     3421 ataggtgcta gaaactctgt tacgatcgga tttgcgatca cgatccttac ttcgatcatc
     3481 ggggttggat tagggatcat ctctggctac tacggaggga tcattgacaa catcttgatg
     3541 cggatcgttg attttatcat gattctaccg atcatgctga tcattatcgt cttcgtatcg
     3601 attatttcca gatacaatgt ctggtccttt atattcatca tgagtgcatt ttattgggta
     3661 gctaaagccc gattattccg aagcaagaca ttatctgaag caagaagaga ttatgtcagt
     3721 gcctcaaaaa caatgggaac caatgatttt aaaatcatgt tccgcgaaat catgccgaat
     3781 ctaagttcat taatcattac taatttgacg atgaactttg cggcaaacat cggtatcgag
     3841 acaactttga ctttcttagg tttcggcttg ccggcaaatg tacctagttt aggtacattg
     3901 atcggttatg caagtaacgg agaagtctta gtgaatcggc aatggatctg gttacccgca
     3961 tcaatcctta ttttagtttt gatgttgagt atcaattatg ttggacaagc attcaagcgt
     4021 tctgcagatg cgcgccaacg gttaggctag tatagaagcg aagggggaaa taggaatgaa
     4081 gaaaacgatt tttggatttg cagcgctgat gtctgctgtc gttcttgcag catgcggggg
     4141 cggaggtaca gatacctctg gaaacaactc tggaggcaac ggcaataaca aagaggaatt
     4201 aacttttcca attgcaacaa aaaataatga tgatgcaatc gaagacggcg aattagaagc
     4261 tgcggtcgca acggacagtc agtttaaagg attgttccag tgggagtttt accaagattc
     4321 atatgatgca gcttttatgg ctccttctca tgaaccgcta tttgcaaacg atgacgattt
     4381 cagaatcaca gacgacggag ctgcaacgct tgatctagat gaagatacga aaaaagcgac
     4441 aatcaaagta aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg cggacgatgt
     4501 gattttccct tacgaaatca ttgggaataa agattacact ggtatccgtt atgacgacac
     4561 tttccgtaac atcgtaggta tggaagaata tcatgatgga agtgccgaca caatctccgg
     4621 aatcaaaaaa atagacgaca aaacagttga aatcgaatat aaagaagtaa acccaagcat
     4681 gcttcaatcg ggtggtggta tctggtcata tgcagcacca aaacatacgt tagaaggtgt
     4741 agcaatcaaa gatatggaat caagcgatca agtacgtaaa aatccagtga cttttggccc
     4801 ttattacatg agtaatatcg ttgcaggaga atcagtagaa tacttgccaa acgagcacta
     4861 ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga tggcaagtgc
     4921 aactgaagca ttgaactcta aattatatga tatgattttc caaatgccaa cagatactta
     4981 cgatacctat aaagaaattg ctggctatac aaacttaggc cgtgaacaaa cttcttatac
     5041 ttatctagga ttcaagttag gtaaatggaa tgcagataca aagagtgtgg aatacaatcc
     5101 agattcaaaa atggctgata aatcattacg tcaagcaatg ggctatgcat tagataataa
     5161 tgcagtaggt gaacgtttct atgcaggttt gagaagcaat gcgacttcat tgattcctcc
     5221 agtattccag tcataccatg atgatgagat gaaaggattc acacaagatc tagacaaagc
     5281 gaaaaaacta ttagatgatg ctggctacaa agatgtggat gacgatggtt taagagaaga
     5341 caaagatgga aataaactaa caatcaactt tgcttcgatg tccggtgggg aaacagcgca
     5401 accattggca gattactata tccaacaatg gaaagaaatt ggtttggatg tccaatacac
     5461 aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg atgatcctga
     5521 aattgatgtt taccaagctg cttggaatac aggaactgat ccaaatccaa gtggactatg
     5581 gggaccaaat gctgcgttca actacactcg ttatgaatca gaagaaaata caaaattgat
     5641 caacgatatc agttcaagca aagcatttga tgaagagtac caaaaagaag catatcaaaa
     5701 atggcaacaa tatgcatttg aagaagcatt tgcgattcca acgttgttcc gtaatgaagt
     5761 attgccagtc aacgaccgtg tcaaagactg ggattggtct tatgatgtac caaacccatg
     5821 ggcaaatgtt tctgttacag cagattcacg ttcataatca ctaaaaatga ccgaaaaagg
     5881 gcgatgtcct ttttcggttt tttgatattt ttctgtttat ttctctaata ttcaggtcaa
     5941 ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa gtccagtttc tcggtattaa
     6001 gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa attttttctt catactcgaa
     6061 actaaacgaa tttttcacaa cttctcgtca atcactagga ttattaaagc tttacttacc
     6121 ttttgaccga accatttcaa atttgacaaa aaaccagtat aataaggaag tatcgaaaaa
     6181 atatccataa aaggaatgtg gaaaatggac aaccagctga caaaagaact aaaagaaaac
     6241 tataacatcg tttttcacga tctaaatttg cttgaacaag cttttaccca ttcatcctat
     6301 gtgaatgagc atcgcaatct gcaattatcg gataatgagc gacttgaatt tttaggagat
     6361 gccgttttag aactgatggt ttctcagtat ttattccgtt tatatcctca tttgccagaa
     6421 ggaaaattga caaaaacacg agctgcgatc gttcgcgaag acagcttgtc gaaatttgcc
     6481 aaagaatgtc actttgatca atatgttttg ttgggcaagg gagaagagaa ttcaggagga
     6541 cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc gttatatctt
     6601 gaccagggat tcgacacgac ccatgcattc atcgaaaaag tgatttttcc taaagtcaaa
     6661 gcaggtgctt tttcacatga gatggatcat aaaacaaaac tacaagaagt cttgcaaaaa
     6721 tcaggtgatg tatcaatcga ataccgattg atcaatgaag aaggtccagc tcatgaacga
     6781 gtattttgga tcgaagttta tgtagatgat caattgattg gcactggaca agggaaatcg
     6841 aaaaaattag cagaacaagc tgcggcggaa aatgcattgg cagcactgta aaagtaagtg
     6901 agggaaagct ttgtatttga aacgaattga aatagccggt tttaaatcat ttgcagacaa
     6961 gacagtcatt gattttgaaa atagcgtcac agcagtggtt ggaccaaatg gcagcggaaa
     7021 aagcaatatt acagaggcta tccgctgggt attaggagaa caatctgcta aaagtctgcg
     7081 aggcggaaaa atgccggata tcatctttgc cggttccgat acccgtaagc agctgaatat
     7141 tgcagaagtc acagtgattt tagacaatag tgatcattat ctgccattgg agtacaatga
     7201 gatcagtgta acccgccgtt atcgacggac tggggaaagc gagtttttca tcaataaaca
     7261 aagctgcaga ttaaaagata tccaagaatt gttcttagat tctggattag gaaaagaatc
     7321 gttctcaatc atttctcaag gaaaagtaga agcgattttc agtagtaaac cggaagaccg
     7381 tcgtgggatc tttgaagaag cagctggtgt cttgaaatac aagcagcgga aaaagaaagc
     7441 agaacagaag ctttttgaaa cagaagacaa cctcagccgc gtccaagaca tcatccacga
     7501 attggaagaa cagttaactc ctttggccgc ccagagtgag gcagcaaagg agtttttacg
     7561 gctaaaagaa acactgactc aaaccgatgt ctctttgatg gttgctgaaa tcaagacagc
     7621 aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag aattaggcaa
     7681 actatctgag tcgatccaag aacaagaatc cattttggcg aagcagcgta aagaaaatgc
     7741 gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt cggaaaaact
     7801 gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg acaaagcata cacaaaaaag
     7861 ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa aaggttaaac attttgaaaa
     7921 gcttcaagaa agcttgatga aagcagcagc agaaaaagaa acagagatcc aaaaagctga
     7981 agcaaatctc ataaaaacac agcaagaatt agaaaaatac caaaaatcaa cgaaagaatt
     8041 attggcagaa ttaagagatc aatatgttga tttgatgcaa gaacaagcag ctgtaggaaa
     8101 cgaactgaag tatctagaac gtcaatatat acaagaaaca gcaaaaagca agcagacgtt
     8161 agccaagcaa tcggaagtag aagcatcagt agaccgattg atcttacaaa aagaagaatt
     8221 gacacaaaag caggcgcagc taaaatcatc tttaactgaa acaaaagaga agttagaaat
     8281 gatccaacaa aacgggaaaa agttccaaga agcattagca aaagaacagc ctaaaatgta
     8341 tcaattaatg aatcaagtcc agcagctacg tgctcgtaag aaaagccttc aagaaatcca
     8401 agaaaactat tttggttttt accaaggggt tcgattggtc ttgcagcata aacagcagct
     8461 ttcgggtatt gtcggagcag tggctgaact gatcgatgtt cctgcagatt tcacgttagc
     8521 tatcgaagca gctttaggtg gggcagccca gcatgtcatc gttgaaaatg aaaaagatgc
     8581 tcggcaagcc atcacgtatt tgaaacagca aagaggagga agagcaacat tccttccact
     8641 gacgacaatc aaaccaagac aattaccggc tcatatattg acacaagcag cagctgtaga
     8701 agggtttata gggattgcca gtgaacaagt cagttatccc gatcaaatcc aaactgtggt
     8761 tcaaaacctg ctaggaacga tccttttagc caaagactta actagtgcca atgcaatcgc
     8821 gcaaacgatc cgctatcaat atcgagtggt ttccttagaa ggtgatgtga tgaatgccgg
     8881 aggatccatg acaggtggag cgaataaacg aggaaaccaa ggcagtttgt ttgtacaaaa
     8941 tcaagaattg aaacagttga ctagtgaatt tgaagaagca gataaacaac tccaagcaca
     9001 agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt ttagccgaag aacaagaagt
     9061 tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa atcagctaca
     9121 aaacatcata aatgaacttg aacgttttga aaaagaaaag caaatctcaa cttttgagac
     9181 acgtgaactg cagcagttta tcgaagatta cgaaaagcaa acgaacgaat tgaaagacaa
     9241 acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat cgttaagtca
     9301 agaaagcgac cagatggaag ccagaagagc acaagtacaa agtcaaaaag cacaagaaca
     9361 agcagatctt gctgtcttga aagagcagtt taatcatctg cagatccagc tccgcggtgc
     9421 ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg atagaaaagc agttggcgac
     9481 attgacagca gacttttctg atcatgaagt cacagaagaa agtttggaaa aacaaattaa
     9541 cgaattatct gctcagcgag aaacattaaa agccgagttg gcaaaagcca aagagcagcg
     9601 agatagaagg caaaaggaga tcgataagct tgaagcagtc ttggcagagc gtaatcgtga
     9661 gcaaaaagca cgactgtcgg aacaatccaa attagaagtc caaaaagacc gagctgaaat
     9721 gattttagat aatcatttga cctatctgca gtcagaatat cagctgacat ttgagaaagc
     9781 ttcccaagac tatcaagaaa caaccgatat cgaagacagt agaacaaagg tttcatcctt
     9841 aaaagaacag attgaaaaac tgggaccagt aaacttaaat tcaatcgaac aatacgaaca
     9901 agtcagtgaa agacatacat tcttagcaac acagcgagat gatctgttag cagcgaaaaa
     9961 ccaattgttt gaaacgatgg atgagatgga tgatgaagtg cggacaagat tcaaagaagt
    10021 atttgaagct atccgtcaag aattcaaaat tgtctttcca aatatgtttg gcggcggtaa
    10081 agctgaatta gtactgacgg aaccaactga tttgctgaaa acaggtattg agattgaagt
    10141 acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag aacgagcact
    10201 tacagccatc gcgttgctct tttccattat ccgtgtctgt cctgttccgt tttgtatctt
    10261 agatgaggta gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc gttacttgag
    10321 cgatttccaa gatgacacac aatttatcgt agtcacacac cgcaaaggaa cgatggtcgc
    10381 agcagatgta ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa tcgtttctgt
    10441 acggatggaa gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt aggaacgaaa
    10501 atgatcaaat tagtagcaat cgatttggac ggtacattat taaatgaaca aaagctgatt
    10561 tcagatgaga ataaacaagc attagcacaa gcaaaacagc aaggggtcaa gatcgttcta
    10621 tgtacaggac gccccttagc tgcgatggcg cattatctgc aagaattagg actggtagat
    10681 gaaggtgact acagcatcac tttcaacggc ggtttggtac aaaaaaatga tactggtgaa
    10741 atcatggaaa agaaagtgat ggaagtatca gatatccaac gcttatacaa actggctcag
    10801 caattggatc tgccactaga tgttttgtct gacaatgtgg ttttacagct gcctagtgcc
    10861 ccaaggaagc aatccttgta caatgtgttg aataagctgc tgcaattcca accagcggat
    10921 ataagcgata ttacagatga atttgtgctt aataaggcag taatcgctta tgaccaagaa
    10981 gaattagatc ctaaaatcaa agaaatacca gcagaattcc atgaaatgta tgagatcatc
    11041 aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc ttatggtatt
    11101 tctttattgg caaaagatct tggtttgaaa caagaagaaa tcatggcgat cggtgatgag
    11161 gaaaatgatt taccgatgat ccaatatgct ggactaggcg tagcgatgga gaatgctgtg
    11221 cctttcgtca aagaagcagc agatttcgtg acagccagca atgaagaaaa tggcgtagcc
    11281 aaagtgatcc aaaaatttgt attagagtag ggggagtcaa atgggatttt ttgataaaat
    11341 caagcgtgct tttactggcg aagagaaaac agaagaagtt gaagtacagg agaagtacga
    11401 caagggttta gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt tgtttgccaa
    11461 ttttcgaaca gtcgatgaag acttttttga agaattagaa gaaacactta tcggtgcaga
    11521 tgtaggtttt gaaacagcga tacgaatcac cgaagcatta cgtcaagaag tgaaactcag
    11581 aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg tggatctcta
    11641 tgaagcggaa ggaatcaatg aagtaaatga actaaatatc caagaagacg gactaacagt
    11701 tattttgttt gtcggtgtta atggtgtagg gaaaacaaca agtattggta aacttgccca
    11761 tcagttcaaa aacgaaggta aaaaagtatt gatggcagca gcagatacct tccgcgctgg
    11821 agcaatcgac cagttagttg tctggggcga acgcgctggt gttgaagttg ttcgtggcaa
    11881 tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa agacagagca
    11941 ggcagatgtt ttactagttg atactgcagg ccgactgcaa aataaagtga acttgatgaa
    12001 cgaattagaa aaaataaaac gagtaatcca gcgggaatta ccagaagcac cccaagaaac
    12061 acttcttgta ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa aacaattcaa
    12121 agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta gatggtacag caaagggcgg
    12181 tatcgtactt gctatccgta atgaattgca tttaccagtg aaattggtcg gtcttggtga
    12241 aggaatcgat gacttggagc cgtttgatcc aaatgatttt gtcgttggat tgttcaaagg
    12301 attactaaaa gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa agttgcagct
    12361 ccttgtttga atgattgttt ttaacatagc tgtgtttttg atgccttcat ttttatactt
    12421 atttgtaagc ggttctcaaa caatttcgtt ctttttctta gactttttga ttataattag
    12481 attcatatga attggaggtc acttaatgat gaattttaag caattattat tgcatgtcaa
    12541 tgcgcgccct tttatcgacc aagctcgatt tggtatagaa cgggaaggac agcgggttga
    12601 tcttgcagga aatctagcaa aaaccgatca tccagcaatc tttggcgatc gatcctatca
    12661 cccctatatc caaacagatt ttagcgaaac acaaacagag atgatcaccc ctgttaccga
    12721 ttctattccc gaattatttc agtatctcgc tgctgtttat gatgtgactg ctcgttctat
    12781 accgaaagaa gagatgatct ggccattaag tatgccacct gccttaccgg aaaaagacga
    12841 agagatcatt attgcaaaat taaaaaattt cgaagatgtc ttgtatcgac gttatttagc
    12901 aaaaaaatac gggaagcgaa aacagatggt aagcggtatt catttcaatt ttgaatttgg
    12961 cgatgagttg ctaagaacat tgttcagcca tcaggaagaa tttcaagatt tttccgaatt
    13021 caaaacagaa ctttatttga aaacagcgag aaactttatg cgctatcgct ggatgatcac
    13081 ttatttattc ggtgcttcac caatgagtga gaagaactac tttttagatg aatcacatcc
    13141 gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc tatacgaatc atccaaatgt
    13201 gaaagtttcc tatgcttcta tgaaacagta tttagcagat atcgagcgaa tgatcgaaga
    13261 agggaaactt tcggaggaga aagaatttta tacaccgctt cgtttccgag gcggaaagaa
    13321 agtcgcagat ttagcaacaa caggtgttcg ttatattgaa ttgcggaata tcgatttgaa
    13381 tccttatgca agattgggta tcaatccaga gcaagttcgg ttcttacaat tattcctgat
    13441 gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa tgggtagcag aaggtacgac
    13501 tcgaaataac aaagtagcac tagaacaacc atcagatcaa acagaatttc atcaagaagg
    13561 tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa ttggattggt tggattctct
    13621 ttatttagta gaggaagcat tgactcaaat ggatcatcct gaacaaacgt tagcagcgaa
    13681 actctatcaa gaagcacagc tatcgagtca gcaagaagtt gctgtggcat tgggacatca
    13741 atattataaa gaaagccatg aacgtcccta tcagttggcc ggttttcgcg aaatggaact
    13801 ttctactcag atatttatgt tcgatgccat ccaaaaaggt gtccaagtca aagtattaga
    13861 tgagtcggat caatttttgc gtctgcaatt ccaagaccat gtagaatatg tgaaaaatgc
    13921 aaacatgaca agtaaggaca gctatatcgt cccacttatc atggaaaaca aaacagtaac
    13981 gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga ggcgcagaat tttcatccat
    14041 ggaagaagcg gtaaaagctt atccgagatt tgccgagcag gcatttgtga tcaaacctaa
    14101 atcaaccaat tacgggttag gtatcacgat tttcaaggaa ggagctagtc tggaagatta
    14161 tcaagcaggt ttagccattg cttttcgtga agacagttcg gttttagtag aagaatttat
    14221 gccgggaacg gaatatcgtt tctttgtgat cgacggagaa gtccaagcca tcatgttgcg
    14281 agtccctgct aatgttattg gtgacagtat ccgaacagtg aaagaacttg ttgaagaaaa
    14341 aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct ttagaattga tccaattagg
    14401 tgaattagaa caattgatgt tgaaagaaca agggttgacg atcgaatcag ttccccaagc
    14461 caatcaaatt gtttatctaa gggagaactc aaatatcagt acaggtggtg attcgatcga
    14521 tatgactgat gaattttctg aagcttacaa aaaaatcgcc gtttctgcgg tagaagcact
    14581 aggagctaag attagtggta tcgatttgat tataccagat aaagaaatcg atcctacaac
    14641 tgataaaaaa gcatatggga tcatcgaagc aaatttcaat ccagccatgc atatgcatgt
    14701 ttatcctttt gcaggaaaag gcagaagatt gacgatgaat gtattgaaat tgttatatcc
    14761 tgaagtattt taaaagaaaa agcttttgac agattgtcct atttgatgtc aatctgatca
    14821 aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt tttaaaataa acgatcccag
    14881 aagctttttt tcttttttgg tttttctttt tcagctggcg aagagttttc ttctgggtat
    14941 tttttttcga tatcaatcac aggttcattt actgcttctt ttgctgttaa gagcaatccg
    15001 aattcctcag gatttgcagt gacgtaatcg ttgactacag tgaaacggct attgtttttc
    15061 gttgctagct ggatatattg gttttggaca gaaaggggta gagccccatt caagagtatg
    15121 gaagcatctg gatgtttttt gagttctttt aaaaagtttt ctttgttttt tggttggatc
    15181 atttcagcaa cagtcatact aagatagcag cgttccctga atgtccctaa atatttatgc
    15241 tgttcttcag ggttcactaa aggtgtacca taaagtccct tatccaaatg gctttttaca
    15301 tcatcttgtg ccataaaaaa taccctcctt cttctctttg tctttcttac gattatagca
    15361 tattcccaat aatacctaaa acatatgaaa aagcggttgc ttttgttcaa cgattcactt
    15421 aaaataagaa tgaaaatcat tctcaattag aaaggtggat tggatgcgct acggacgact
    15481 agtagcatta ttagttattt taagtagtat ttcattgttc atcggagtat cgaatgtaca
    15541 attgtcagaa ctgctccagt tagatgggca aagcttacaa atcttgctag tgactcgttt
    15601 gccacgaacg atttgtttga tccttgtggg agcgaccagt agtatttgtg ggttgatcat
    15661 gcaacatttg acccaaaata aatttgtttc accaacgact gcgggcacga tggatagtgc
    15721 gcgtttaggt attttagtgg ctatgatttt cctacctggt gcatccttga ttgtccgatc
    15781 cttgacagca ttttgctttg cttttgcagg aacattgctt tttttatccc ttacgcgatt
    15841 gttcctccag aaaaatcaat tgatgcttcc gttgattgga gtcatgttag ggaatatcat
    15901 tggttcagtt gctacttttt ttgcttatca attccagttg gtacaaaata tgacttcatg
    15961 gcttcaaggg aattttgcga ctgtgatacg aggaaattat gagttattgt atgctgttgt
    16021 tccattgttg ctcttggttt ggctatttgc ttaccgattt acgatcattg gtctgggcga
    16081 atcgtttgca gtaaatctgg gaatagatta ccagaagatg cagtttttag gcattggctt
    16141 agtatcattg gctagtgcag tcatgttgat catggtagga agcattcctt ttatcggagt
    16201 gatcattccg aatcttgtct ctagaatata tggagatcaa gtgcacaaaa caattggcgt
    16261 aactgcttta gcaggtagtt tgttcttagt attttgcgat cttgtcgctc gcgtgttgat
    16321 ttttccttat gaaataccag tcagtgtgat cgttggaatc atcggtgggg ctatcttcct
    16381 ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa gccatctatt tttattggta
    16441 tgcttgttct tgttttattt gttacaggaa tgtacttgac gatccataca tatggaaact
    16501 ggcattttgc atgtgcattg aggagtaaga aaatttttgc atttatcctt gtctcgctag
    16561 ctgtcagcag tgccacaatc agctttcaga caattacgca taaccagttt ttgactcctt
    16621 cagtattggg acttgagtcg ttatatgtcc ttgtccaaac ggtccttttc ttctttatcg
    16681 gaggagtgac gatgcttcag cagacaggta cagttttttt cttattgaac gttgggctga
    16741 tggtcgggct gagtctattg ttatttttct ttcttttgaa aaaaatgaga gcgaatcttt
    16801 ttttattact gatgtcaggg atgattttag gtacgctttt tggtagtgtc agtacctttt
    16861 tacaagtcat catggatccg aatgaatatg atttgcttca aggcagatta tttgcaagct
    16921 ttggcaatat ccagagtcag tatcttttgc ctgcttcttt gatcattatc ggctgctggt
    16981 acctgttatt tcgatatcga catgtgctag acgttttcca tctcggaaat gaacaagcag
    17041 taaacctcgg gatagacaca gatcgttttg aatttttgat tttatgttgt gtcagtcttt
    17101 tgacagggac atcaacggct cttgtgggtc cagttacttt tttagggttt attgtcgcaa
    17161 atatcagtta tcgtgtcttt tctacttatc gtcatggtca gctatttatc ggaagctttc
    17221 tggtagctat gctgtttctg acaggaggac agctcgcagt agagcagatt ttcggctgga
    17281 atacaacagt gagtgtcgtc gttgaatttg tcggaggaat ttattttgtc acaaaattga
    17341 ttcgcgaaag gaagagtatc ctatgattga tttacaaaag gtcaataaac tgtaccatgg
    17401 aaaatcagtg gttaaagatg tacagttaac gattcctgaa aagaagttga ctgcttttat
    17461 cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg atcagtcgtt tgataccaaa
    17521 agatacaggt gaaatctatt tagaccataa cgaagtaaag gcttggaaat ccaatgaatt
    17581 agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata aatgtccaaa tcaccgtgaa
    17641 agaattagta gaatttggac gatttcctta tacgaaagga aaattgacga caaaagacga
    17701 ggaaatcgtt caggaatcac tggaacacct tgggttagaa gaaatagcgg atcaatcgat
    17761 tgacactctt tcaggcggtc aactccaacg tgcgtatatt gcaatggtgc tagcgcaaga
    17821 cacggattat attttactgg atgaaccttt gaacaatcta gatatgaatt ttgcggttca
    17881 aatcatgcag attttaaaaa atttagtaca agaaatcggg aaaacaattg ttattgttct
    17941 tcatgatatc aactttgctg caagttatgc agacgaaatt gttgcaatga aagatggaca
    18001 gttatttacg caaggaacga cccaagaaat gataaaaaaa gatgtattgg atgaattata
    18061 cgaaatggac atacggattt gtgagattga aggccgcaga ttctgtcttt atttctaaaa
    18121 ctaacattag gagagaagag atgaaaaaat tagtagtagg tatcttgatt ctagcatcat
    18181 ttggtttagc agcatgtgga aatagcaaca acaccagtca ggcagacaca aaaaaaagca
    18241 gcacacaaac tgaaactaca ttgacgataa cagatagtaa tggagaccaa atcgaggtgc
    18301 caaataatcc agaaaaagtc gtcgtattcg acaatggttc tttagatacg atggatgctc
    18361 ttggtgttgg agataaggta gttggagcag ctacaagcag ccttcctgag tatctttcat
    18421 cctataaaaa agtagaatca gcaggtggaa taaaagaacc agacttagaa aaaattaatc
    18481 agcttcagcc agatctaatc atcatttctg gccgacaaag agattttcaa aaagatctaa
    18541 gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa aaatccatgg gaatctttcc
    18601 agcaaaatgt cactgcttta ggggagatct ttggtaagca agaagaagct aaaactcaat
    18661 tagaggaatt atctagtgca atcgatcaga cgaaaaagaa agcagaagct actgacaaaa
    18721 aagcactagt cacacttgtg aatgaagggc aattatcagc ctacggatct ggttctagat
    18781 tcggtttcat ccatgattta tttggctttg aacaagcaga tgatcaaatc gaagcatcca
    18841 ctcacggtca aagtgtttct tatgaatatg tgctggaaaa aaatccggat atcttatttg
    18901 tagtagacag aacaaaagct attggtggag acgacagcaa agatgatatt tctgccaatg
    18961 aactggtagc acaaacgaat gctggtaaaa atcagcagat catctccctt gaaccagatg
    19021 tctggtattt aagcgggggc ggtttagaat caatgaaact gatgatcgaa gatgtgaatc
    19081 aagcatttaa atagcataaa taacagagga ttaaactctc atactatgta acaataatgt
    19141 ttcttacatt cttaaatagc aagagcctat cctcaaagaa gttatcaaaa accatccgta
    19201 aaatcgtatt gatgatttta cggatggttt ttatttattt gagaaatctt ttttcaacgt
    19261 gaattcatta tctacatcat acgggttgat tctagaatgg ggagtggcaa aaaatagtgt
    19321 gatccatttt ttcagcatca agatcgcctc ctttgatttg aatcaaaggt acaaagcgaa
    19381 acttagataa aacttaaatt tagatatttt tattttgtac cgatctcttc tttaaacgat
    19441 gttccatctg ctgcttctcg gcaataagtc cactagacta gaaaatatga gaagctattt
    19501 tctgtccagc gttcttatta ctcaaaacaa atcgcagttg tcacaatctc ttctaaacga
    19561 tgttccatct gctgcttctc ggcaataagc ccactagact agaaaatatg agaagctatt
    19621 ttctgtccag cgttcttatt actcaaagca aatcgcagtt gtcacaatct cttctaaaca
    19681 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
    19741 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
    19801 gggaagctta ctccaccgtt ttagtaattg atagaaataa ctctcttctg ctttgatttt
    19861 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
    19921 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
    19981 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
    20041 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
    20101 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
    20161 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
    20221 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
    20281 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
    20341 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
    20401 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
    20461 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
    20521 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
    20581 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
    20641 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
    20701 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
    20761 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
    20821 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
    20881 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
    20941 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
    21001 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
    21061 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
    21121 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
    21181 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
    21241 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
    21301 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
    21361 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
    21421 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
    21481 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
    21541 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
    21601 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
    21661 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
    21721 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
    21781 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
    21841 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
    21901 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
    21961 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
    22021 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
    22081 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
    22141 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
    22201 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
    22261 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
    22321 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
    22381 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
    22441 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
    22501 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
    22561 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
    22621 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
    22681 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
    22741 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
    22801 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
    22861 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
    22921 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
    22981 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
    23041 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
    23101 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
    23161 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
    23221 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
    23281 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
    23341 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
    23401 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
    23461 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
    23521 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
    23581 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
    23641 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
    23701 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
    23761 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
    23821 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
    23881 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcgctc ttctcggatt
    23941 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
    24001 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
    24061 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
    24121 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
    24181 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
    24241 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
    24301 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
    24361 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
    24421 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
    24481 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
    24541 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
    24601 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
    24661 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
    24721 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
    24781 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
    24841 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
    24901 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
    24961 ttagattcta cagctcatcg tggaatcaga ataaatattc ctgtagaaaa ccaaagaatc
    25021 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
    25081 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
    25141 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
    25201 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
    25261 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
    25321 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
    25381 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
    25441 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
    25501 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
    25561 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
    25621 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
    25681 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
    25741 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
    25801 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
    25861 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
    25921 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
    25981 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
    26041 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
    26101 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
    26161 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt
    26221 ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag
    26281 gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct ttttataaca
    26341 atataaaata taccatttta ttaataaaaa tgcatactag taaatgatat aattctaata
    26401 acaggtttga tcgcgagaaa ggccaaataa aacgctctcc aaaatgttaa aaaaaagaca
    26461 ggacgatgta tattatacaa acaagaatcg ctgacaacag tgactcggat ccaggaaatc
    26521 agttttatcc ccagctagtt ggtgaatgca tggaaagtaa tatacgtaga aagagctgat
    26581 gactaatgaa ttatgagttt tttttaagca aagaagatca cagaagttat tcattactta
    26641 aatatttaga agcaagctca aatttatcag agtcaatatc gaatgttcaa gaagagttgt
    26701 cgctatcgac atttctcttg aaaaaaacga tcgataagct gaaacatgat ttacaaaagt
    26761 tcgatctaga gaaaaatttg cgagtaaccg tcagtgaatt ggatatatgt ctggaaatta
    26821 atggaaaatg ttctagcaaa gctctgttat ctcgttatat aacggattct ttatctatga
    26881 aaatggtatt ggctttcttt caggaaaaat atatctcagt agaaaatttt gctaaacagc
    26941 atcacgtgag ttattctgtg gcttataaag tattacaaag actaaaaaga aatctaaaaa
    27001 aatatcaaat tttttttgaa a
//
LOCUS       DAZBWR010000026        26160 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000026 DAZBWR010000000
VERSION     DAZBWR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.026"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHGT5_001017"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHGT5_001017"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN7217021.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACHGT5_001018"
     CDS             1286..2461
                     /locus_tag="ACHGT5_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN7217022.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACHGT5_001019"
     CDS             2465..3106
                     /locus_tag="ACHGT5_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217023.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACHGT5_001020"
     CDS             3103..4020
                     /locus_tag="ACHGT5_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7217024.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACHGT5_001021"
     CDS             4017..4688
                     /locus_tag="ACHGT5_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217025.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACHGT5_001022"
     CDS             complement(5229..5717)
                     /locus_tag="ACHGT5_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN7217026.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACHGT5_001023"
     CDS             complement(5918..6184)
                     /locus_tag="ACHGT5_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN7217027.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACHGT5_001024"
     CDS             complement(6184..6447)
                     /locus_tag="ACHGT5_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN7217028.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACHGT5_001025"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACHGT5_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACHGT5_001026"
     CDS             complement(7250..9073)
                     /locus_tag="ACHGT5_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN7217029.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACHGT5_001027"
     CDS             complement(9671..12376)
                     /locus_tag="ACHGT5_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN7217030.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACHGT5_001028"
     CDS             complement(12682..13629)
                     /locus_tag="ACHGT5_001028"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN7217031.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACHGT5_001029"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACHGT5_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN7217032.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACHGT5_001030"
     CDS             complement(14264..14626)
                     /locus_tag="ACHGT5_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN7217033.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACHGT5_001031"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACHGT5_001031"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN7217034.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACHGT5_001032"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACHGT5_001032"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN7217035.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACHGT5_001033"
     CDS             complement(16254..17765)
                     /locus_tag="ACHGT5_001033"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN7217036.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACHGT5_001034"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACHGT5_001034"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN7217037.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACHGT5_001035"
     CDS             complement(19592..20668)
                     /locus_tag="ACHGT5_001035"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN7217038.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACHGT5_001036"
     CDS             20864..22609
                     /locus_tag="ACHGT5_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN7217039.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACHGT5_001037"
     CDS             complement(22889..23326)
                     /locus_tag="ACHGT5_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN7217040.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACHGT5_001038"
     CDS             complement(23310..24683)
                     /locus_tag="ACHGT5_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217041.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACHGT5_001039"
     CDS             complement(25005..25493)
                     /locus_tag="ACHGT5_001039"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7217042.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACHGT5_001040"
     CDS             complement(25515..26117)
                     /locus_tag="ACHGT5_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN7217043.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7504 a         5561 c         4837 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat caggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAZBWR010000027        25680 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000027 DAZBWR010000000
VERSION     DAZBWR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.027"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACHGT5_001041"
     CDS             279..1127
                     /locus_tag="ACHGT5_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN7217044.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACHGT5_001042"
     CDS             complement(1298..1639)
                     /locus_tag="ACHGT5_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFN7217045.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACHGT5_001043"
     CDS             1725..2432
                     /locus_tag="ACHGT5_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN7217046.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACHGT5_001044"
     CDS             2419..2742
                     /locus_tag="ACHGT5_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN7217047.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACHGT5_001045"
     CDS             complement(2831..3868)
                     /locus_tag="ACHGT5_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN7217048.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACHGT5_001046"
     CDS             complement(4315..5298)
                     /locus_tag="ACHGT5_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN7217049.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACHGT5_001047"
     CDS             complement(5714..6484)
                     /locus_tag="ACHGT5_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217050.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACHGT5_001048"
     CDS             complement(6477..7412)
                     /locus_tag="ACHGT5_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217051.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACHGT5_001049"
     CDS             complement(8006..9655)
                     /locus_tag="ACHGT5_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN7217052.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACHGT5_001050"
     CDS             complement(9850..10746)
                     /locus_tag="ACHGT5_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN7217053.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACHGT5_001051"
     CDS             complement(10739..11419)
                     /locus_tag="ACHGT5_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7217054.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACHGT5_001052"
     CDS             complement(11420..12202)
                     /locus_tag="ACHGT5_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN7217055.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACHGT5_001053"
     CDS             complement(12195..13112)
                     /locus_tag="ACHGT5_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN7217056.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACHGT5_001054"
     CDS             complement(13115..13399)
                     /locus_tag="ACHGT5_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217057.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            14187..17414
                     /gene="fss3"
                     /locus_tag="ACHGT5_001055"
     CDS             14187..17414
                     /gene="fss3"
                     /locus_tag="ACHGT5_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN7217058.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            17526..17840
                     /locus_tag="ACHGT5_001056"
     CDS             17526..17840
                     /locus_tag="ACHGT5_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN7217059.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17853..18227
                     /locus_tag="ACHGT5_001057"
     CDS             17853..18227
                     /locus_tag="ACHGT5_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN7217060.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            18228..18581
                     /locus_tag="ACHGT5_001058"
     CDS             18228..18581
                     /locus_tag="ACHGT5_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN7217061.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            18650..19666
                     /locus_tag="ACHGT5_001059"
     CDS             18650..19666
                     /locus_tag="ACHGT5_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN7217062.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            20260..22134
                     /gene="ltrA"
                     /locus_tag="ACHGT5_001060"
     CDS             20260..22134
                     /gene="ltrA"
                     /locus_tag="ACHGT5_001060"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN7217063.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            22236..22616
                     /locus_tag="ACHGT5_001061"
                     /pseudo
     CDS             22236..22616
                     /locus_tag="ACHGT5_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            22661..23803
                     /gene="dcm"
                     /locus_tag="ACHGT5_001062"
     CDS             22661..23803
                     /gene="dcm"
                     /locus_tag="ACHGT5_001062"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFN7217064.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            23793..24082
                     /locus_tag="ACHGT5_001063"
                     /pseudo
     CDS             23793..24082
                     /locus_tag="ACHGT5_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24338..25522
                     /gene="mobT"
                     /locus_tag="ACHGT5_001064"
     CDS             24338..25522
                     /gene="mobT"
                     /locus_tag="ACHGT5_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN7217065.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            25519..25632
                     /locus_tag="ACHGT5_001065"
     CDS             25519..25632
                     /locus_tag="ACHGT5_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN7217066.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         8057 a         4994 c         5032 g         7597 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    13921 cattgaataa gtttgaaaag ccttgattta taaaggtttt tccgtaaata tattttgtta
    13981 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata
    14041 tttcgtattt tatattaaat ttttaggctc attcattctc aaatccattc tatttagtca
    14101 ataaatattt taaaaagact ttataaacaa agatgttcaa gcgagcatct tatttttttg
    14161 tgcctaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat
    14221 taactatttt gagtttggct gttcaatcaa ttgtaggagc aattgctcct acgattgctt
    14281 ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta gcacatcaat
    14341 atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact gttccgcatt
    14401 atgcagttta caatggtgtg aagcaagata ttttctgtgt tgaaccagga gtcccgattt
    14461 acaatgagtt cactcctgga tatgagaaga acccgttacc agatatgtcc gataaagcca
    14521 aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat attgtggcac
    14581 agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa cgatcagatg
    14641 gtagtgccgt aaatatcgca gccattgagg caaaaatcaa tcaagccatt gccgattatc
    14701 agaagaaacc aagcttccat aatagtacgg ctaaaacagt attagggcaa tcgactactg
    14761 tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa aataccgcaa
    14821 acattgatta tcgtgtgaat ggcaatcagt tgataatcag cccaaatgcc aattcaaaag
    14881 aaagtggtgt gttgacgctt aagaaatctg ctggtaccgg aacacctgtc gcctataaaa
    14941 aggctggcca acagacttta atggccgggg cgatcgataa gccgaacact tatacggtca
    15001 aaattgatgt ggaaatggag ggttctttga agattaaaaa agttgataaa gaatcgggtg
    15061 atattatacc aggaacggtt ttccatttag actttggaaa aactttaccc gcaaaagacg
    15121 tgaccaccga taaagaaggc attgcgacct tagatgagat tcctcatggt acaaaggtaa
    15181 ccattactga aaaatcggtg ccggcgcctt atacgattga taccacaccc atgactgcca
    15241 caattaaagc aggtgaaacc atttcagtga cttcaaaaaa tacgcgagaa aaaggtcaaa
    15301 ttattctgga taaaaccgga attgaaacag ggagtgatct ttggaacgat aattactctt
    15361 tagcgggcaa tacgtttgcc attcgcaagg atagcccaac tggtgaaatc gtccaagaaa
    15421 tgaccacgga tgaaaaaggt catgcagaaa caccaaaaga gattgccaat gcgttggaat
    15481 tgggaaccta ctatgtgact gaaaccaagg ctagtcatgg ctttgtgaat actttcaaac
    15541 cagtaaaagt cgaattgaat tatgtcaatc aaacagtggc tcttgttacc agtaacgtaa
    15601 aagggcaaaa ccaagaaatt actggtgaaa ccactttgac aaaagaagac aaagataccg
    15661 gtcatgaaac tcaagggaaa gctgagttta aaagagctga atacaccttg tttactgtta
    15721 aggatggaaa agcggtcaaa tggaccgaga actttaaacc agaattgttg aagggaacga
    15781 aagcttctga tgaaaccgta actttgactt tagatgaaaa gaatcaagtt gccgttaaac
    15841 acttagcgat taatgagtat tactggcaag aaaccaaagc gcctgaagga tataccttgg
    15901 atgaaacaaa gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa aatgctgtga
    15961 ttactcgaga tgttgcggca aaagaacaag tgattcgctt tggctttgat ttctttaaat
    16021 ttgctggatc aactactggc actgccgaaa ctggcttcaa tgatctaacc tttaaagtgt
    16081 cgccattgga aggaaccaat gaaatcaccg gtgccgaaga tgaagcgacc acagcttata
    16141 acgagcaatt agggtttgat ggttatggca agtttgaaaa tcttccttat ggggattatt
    16201 tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt
    16261 ctacttttaa agaaaataaa gaggattata ccaagagtga ttatatcttt actattaccg
    16321 aaaaagggca aaagcaaccg attaaaacgg taacggtccc ttatgggaat ctaaccaatg
    16381 agaatttctc agtcagtttg aaccgtttga tgctttatga tttgcctgag gaagaagata
    16441 gcttaacttc tcttgcgact tggaaagaag gagataaaga attgactact cttgattcta
    16501 ccgagctagt cgataaattg agctataatc tacatgaaat caaagaagac tggtatatcg
    16561 tagctcaagc catcgatgta gatgctacaa aagctgccca agaaaaagat aaaaaagcca
    16621 aaccggtggc gattgcggaa acaaccgcaa ctttagcgaa taaagagaaa acaggaactt
    16681 gggagattct gcataaatta actgtcgaac aagttttaga caagaccatt gtcttattca
    16741 actatgtgta tgaaagcaaa gaagcctatg aagctggtga tgagccggta gcgaaggatg
    16801 ctagcttgaa caatcaagcg caaactgtta attgtatggt ggaacgtcat gtttccatcc
    16861 aaaccaaagc gcacttagaa gaaggttctc agacctttac tcatggtgac gtggtagata
    16921 tgtttgatga tgtatccatt acccatgatg tactagatgg ctcaaaagaa gcttttgaaa
    16981 caattctgta cgctttattt ccagatggta caagtaaaga aatttggaaa tctggcaaga
    17041 ttgattacga agtgaatgac aaagaattta ccaaaaccgt acttgcggaa aaagtggata
    17101 caagtaaata tccagaaggt acaagtttta ccttcaaaga aattaactat gacaaagatg
    17161 ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaact ttaacgccca
    17221 aaaaagttcc gacaacacca agtacaccag aacaatctga aatgccaact gttccaagtg
    17281 gctctcaaga atctagtcct acagtgaagg cattccctca aaccggggag aaaacttcca
    17341 atgtgctact gttcattggc tttagcttga tctttgcgac ggctggctat tatttttgga
    17401 atcgtcggaa ctaaggtgat aagatgaaac atgtaaatag tagaaaacaa tacgcgcgac
    17461 cgcctcctaa aaaagtggtc gcgctatttt tgtattcaaa actaacaatg aaaagaggaa
    17521 aaaatatgga attgaaattt gttgtaccag atatggtcga gacgttcgga aaactaacat
    17581 acggtggtga aggtgaagtt ctatcggaag ggtatggtcg aaatactaca attgttggtc
    17641 gaagttacca tctgttttca aataaacaac gggccgatga tattgaagtg gtggtagctg
    17701 cggaagccgg cgaaaaggat tttgaccaag atcaaccact aaaagctgta aatccccact
    17761 taattgccaa aggctatgaa attgagaatc gtgggtttac cgattacgtg ttgtatgttg
    17821 atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag
    17881 attcaggatt aacctttggc aagttgcgtt tttccgcatt acgtcgagaa gtacgcaagc
    17941 aaaatgagga cggaacggtt agtaacgagg taaaagaacg gacctataat ttgaaatcct
    18001 ccgcgcaagg tcgaatgatc caagtcagta ttccagcaaa tgtaccctta cgcgagtttg
    18061 cttatgatgc agaggtagag ctggtcaatc caatcgtgga tacggtcgcc aattatgttt
    18121 ttcgggaagg aaccaccgtt aattggttta tcaaggcaga tgatttagtc ttgaaacgac
    18181 aactgaatca aggaaaacct acgaaccaaa atgaaggaaa gaaataggtg aagaatatgg
    18241 aaattaatgt tgaacatatt ttagattgtc tcgatcaatt tggcaaaggt gagctaacgg
    18301 aggaacaact cactaacgca ttaacctatg atgaaaaaat gtttctgatt atgcatcaag
    18361 ggttactgga agtaggtaac gatgccaaag aagactttga tgtactggat tggttagggg
    18421 aagaagagtc tttctttatg gttgtcgagg taaatgaaag tttatgtcgg caggcagaat
    18481 ctgttttgga agaaatcgat gtggagatgc cggatgccat cgaaagattt ttaaaacaat
    18541 tagtggaaac gaaacaactt cccgtagtag taaatgacta gcataacttt ttgaaattgc
    18601 ccaatgaaat cctattgggc tttttcgact ggaggtggaa accatcaaaa tgtacaaagg
    18661 acatcgcata cgcgcaggag atcaacactt gatttatcac tttgttcttg gctggctaat
    18721 ctttttgttc attggttgga tgagcgtctt ttatttacaa gaacttagac aatttgatat
    18781 ttctaaggta tcattttcca ctagtgaaat tggctggtct atgaaggatg taatctgctt
    18841 gctaggaagt ctcgctcttt caggaagcat gatgttactt tatatacact tttttcagga
    18901 tcattggcga agtctttggc atcgacaaaa gttagctcgg atgattctag aaaaccattg
    18961 gtatgaagta aagcagactc aaggtgaagg ttttttcaaa gatttgaata gtagttggac
    19021 caaagagagt atcagttact tccccaaaat ttattatcga atgaaagatg gcttactttc
    19081 tattcgcgtt caaatttcac tgggaaaata tcaggatcag ctattgaagt tggaaaataa
    19141 gttagaaagc ggactgtatt gtgagttagt agaaaaagaa ctcaaagact cctatgtgga
    19201 atacaccttg ttatacgata tgattgccaa tcgaattggg atagaagaaa tagtggcaga
    19261 aaatggggcg ttgcgattga tgaaaaatca agtatgggcg tatgattcct tgccgcatat
    19321 gttaattgct ggcggcacag gtggtgggaa gacctatttc ttactcacca ttattcaagc
    19381 actattgaag tcagatgcag aattgtttat cctcgatccc aaaaatgcgg acctcgccga
    19441 tttaggtacg ataatgcctc acgtatactc ccaaaaagaa gaaatttctg aatgtgtgga
    19501 agatttttac gaacgcatga tagctcgtag taggtcaatg aaagaaatgc ccaactacaa
    19561 aacaggagag aattatgcgt atctcggact tccgcccaac tttttaatct ttgatgaata
    19621 cgtggcttat atgggtgcga atcgttttcc tacaagcatc gaatagatgt gaaatggaag
    19681 cctattgaga ttatcaatag tgacaactaa tgttttgaga gatggaatga aagccttcaa
    19741 gttgagctga aacatccccg ctaatactcc tacatgctct aatcaatgaa gtagattggt
    19801 tttggtatga agctaggtga agtcgtctga aatctgccta agtgcttgag cataaggctg
    19861 actatagggc gggcggctga aaaagataac tatggtaaga atgtctgaaa atgactgacg
    19921 aatctgcgaa tgtacgggtc taaaaggctg atcttattga aagataggaa catacggacg
    19981 tatggtgaga taccgacaag taaggttcca tgatatgaaa atcgggatag ctaacgaaga
    20041 agctataatt tcacaggctc atagtagata cctaaggtta tacatgatag ctaaaaacat
    20101 tggaacgatg taagcagaaa actatcaatt aaatgactac ttctagatag gtgcatataa
    20161 gtaataacga aattgctttg tttctgtgaa agtaagggca cagtaccgat gaagcgtcta
    20221 acaaatgtgg agggatagcc ctaagtctat ttaataacaa tgaatgttga aacagatgaa
    20281 gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg aggtgccacg cttgaacaca
    20341 aaaatacgtt actgggaata ttatgatcta caagaaacat tcgatcagct atttactcaa
    20401 agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa tcatttctga aaataatatt
    20461 cttttagcat accgtactat caaggcaaac aaggggtctt cgacaccagg aacggattcc
    20521 ttcacaattg ataactataa ggaaatgaac caagcagagt ttatccatct tattctcagt
    20581 catttagaaa attacaaacc aaaatccata aagcgagtaa tgatacctaa acccaatggt
    20641 gagaaacgcc cattaggaat tccatgcatg attgatcgaa tgatccaaca gatgttcaag
    20701 cagatactgg agccaatatg tgaagctaag ttttatgaac atagctacgg tttcagacca
    20761 ttgagaagtg caaagcatgc tctaggtcgc attatgtatc tcataaacat cagtaaaatg
    20821 cactatgcag tagacattga tatcaaaggt ttctttgata atgtaaacca ccgtttactg
    20881 ataaaacaat tatggaatat aggaatttgt gacaaacaag tcttagctat tctttcaaaa
    20941 tctctgaaat cacccattca aggagaaggt atcccgagca aaggaacgat ccaaggaggt
    21001 atcatttctc cactattatc taatgttgtt ctaaacgact tagaccattg ggtctcaaag
    21061 cagtggcata cctttgaaac aaagtatccc tatactaaag gttacaataa atttcgtgct
    21121 ctaagagata caaatctaaa gcaaggctat attgtcaggt acgcagatga ctttaaaatc
    21181 atgactaatg attatccgac agcattaaaa tggttccacg ctgtcaagct ttacctaaaa
    21241 gacagactta aattagatat ttccaatgag aagtctaaaa tcgtcaattt gcgtaaatgt
    21301 aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa aagggaaaaa atgggtttgt
    21361 aattcacgta tttctaacaa aaaaaaggat cagattaaag aagagattaa acaaagaatc
    21421 aaagatattc aaaagagtcc tacagcacaa aatgctctcc tgtttaatgc tctcgtgctc
    21481 ggtgttcata attatttcag gtatgccact catattaatt tagatttgaa taggatgaac
    21541 tacgttcttt cattcactac ttacaaccga ttatcaaatt gtagtacaag atgttacccc
    21601 aacaaagctt ccccaactta caaaaagttt tactcaacaa cgatgaaaac gtataaagta
    21661 gctggagtct atttgtaccc tctttgcgac gtatctacaa aaacaattta cggatttaat
    21721 acggaggata caccgtttac tccgtttggt cgtcaaagat tggagcacaa aagcctacag
    21781 tctcttgtgt tccaagaatt aagaaaattg atggaatcga aaataccgaa tagaacggtt
    21841 gagtatttag atagtagaat atctcgttac tcaatgaaat caggcaagtg tgagattaca
    21901 aaacaatttt tgccagcaaa agccgttcac tgtcatcact atttgccaaa aagtcttggt
    21961 ggagacgaca aattcgataa cttgagaatt attcataagg atatccatct tctgatccat
    22021 acaacaaata aaatgataat cgatcactac gtgcatgaac tgaaactggt gccagagcaa
    22081 atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc aaaacattca atagaaaata
    22141 aatagatgga acgccgtatg aggtgaaagt ctcacgtacg gtgtggagcg ggggaaaaga
    22201 tggagattat atcaaattct tacctatcgc tatagatgtt aacgacaaaa gaaagtgcgg
    22261 tgattttgaa taagcttaaa caaatcgtga tgttaggccg tcaatctggt ttctttctga
    22321 ttctagcttg tcaaaggccg gatgcgaaat atctaggaga tgggattcgc gatcagttta
    22381 acttccgtgt ggctttgggt cgtatgagtg agcttgggta ttccatgatg tttggagaag
    22441 tcgataaaaa tttttttatg aaacgcatca aaggacgagg ttatgtagat acagggggca
    22501 gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc ttaagggaaa
    22561 ttagcaacat tgttggatta accgttcata cggaaagaga aaataatagt gtgtagccta
    22621 ggaatatcaa taatcctggg cttttttatt gaggtgattt atgacatttt tagatttatt
    22681 cgccgggatt ggtggctttc gcttaggtat ggaacaagcc ggtcaccagt gtatcggttt
    22741 ttgtgaaatt gatgaatttg ctcggaggag ttataaagca atccatgata caaggaagga
    22801 ggtggaaatg catgacatca caagtgtatc agacgaattt gttcgatccc tcggaccagt
    22861 ggacatcctt tgcggcggct ttccgtgcca agctttttca attgcgggaa agcggcaagg
    22921 attttccgat actcgaggta ctttattctt tgaaatcgct cggttcgcca ctcttctcca
    22981 acctaagttt ctattccttg agaacgtccg gggattgctc aatcacgaag gaggggctac
    23041 gttcgagacg atcctccgaa cgttggatgg attggggtat gatgtggaat ggcaagtgct
    23101 taactcaaag gcctacgttc cccaaagccg tgagcggatc ttccttatcg gacattctcg
    23161 agatgcatgt accgaacaag tatttcctat cattggatcg tcttcaacat ctgatcaaaa
    23221 catcagaaac ttggtaaata tcaatccttc caatcgagga atgggtggcc aagtctatgg
    23281 ttcagatagc gtagcaccaa ccttgaccgg tgatgaagga attaaaattg ccttaccggt
    23341 aaaggatggg atttctgtag cgggtatgtt gtcgggaaat tttgaacaag gcaataggat
    23401 ttatgaggta accggaacag cccccacttt gtcaacgaaa caagggggaa cgaagattat
    23461 gattcggaaa aatgaaacag aatatcaaga ggtgaagcca ggggacagtg tgaacctggc
    23521 ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa caaagtgtgc ataccttact
    23581 aacaggagac caacaagcgg tagtcacgga tcagtaccag attcgaaagt taacacctag
    23641 ggagtgctgg cggcttcagg gctttccaga ttgggctttt gatcgagctt ctcaagtcaa
    23701 ttcagatagt cagttgtata aacaggccgg aaactcagta acagttccgg tcatttttga
    23761 tattgctaga agattaaagg gggtaaaagc atatgattta tgacgagttg attggagaaa
    23821 tctactggat gattggcaag attcaggcag atccgaaatt ggaagaagaa ctgtaccggc
    23881 tcaattttga tattcgcaaa aacggggtga aagtccaggt gattcgtatg tagtggatga
    23941 ggaaacggat gctcgaatgg agctcaatca agtcatcggt gaattggaac gaattgcgga
    24001 ccttgccaaa gaacctgata tccgccaata cttgtttgag ataaaagcag agttggaaat
    24061 tgagggtatc accgcggagt aatagggaaa taacccactg ataggcactg accgcggggg
    24121 atttgcgaag cagaaatcct ccgtggtgct gtgcctgttc atggacggcg gcagccggcc
    24181 atgatgtctc accccccgta tctaacaggg gggtacaaaa acacaaaaaa caagcaagta
    24241 accataaaaa atgcttgttg tatcaaggat attaagtaat ttgtataggt gttaactcat
    24301 ttggaaaagt taacaccttg aatttggagg tgaaaacttg gcgcaacgaa atttagatta
    24361 tcggttatta aaagaacgac gcaacgaata tggcgtttca caaaataaat tagctactgc
    24421 ttgcggactt agccgcccat acctaaatca gatagaaaac ggtggcgtaa tcgcctctac
    24481 aaagaccatg agaaaaattt ttgatcaact ggaaagtttc aatcccgatt tacctttaac
    24541 gttactattt gactatgtaa ggattcgctt tcccacaacg gatgtgcgga aaattattca
    24601 agaaattctc catttgaaat tcgagtatat gcttcatgaa gattacgcct tttactccta
    24661 tcaagaacaa tatgtcatgg gcgatattgt agtgatgtta tctcatgaag aagataaagg
    24721 tgtccttttg gaattaaaag gtcgtggttg ccggcagttt gaaacttttt tactcgccca
    24781 aaagcgtagc tggtacgact tttttgaaga ttgtctaaaa gccggtggcg tgatgaaacg
    24841 cttggattta gcaatcaatg atcgagtggg gctactggat attcctgatt taacgaaaaa
    24901 atgtcagaag gaagaatgta tctccttgtt tcgtaccttc aaaagttacc gttccgggga
    24961 actgttgaaa gctgatgaaa aggatggtat gggcaatacc ttatatattg gcagcctcaa
    25021 aagcgaggtt tatttttgtt tgtacgaaaa agattatgaa caatatatca agttagggat
    25081 tccattggac cagaccgaaa cgaaaaatcg ttttgagatt cggttgaaaa atgaccgtgc
    25141 ttattatgcg attcaagatt tgttaaaagg tcgtagcatt gaaagtacca cgttttccat
    25201 tatcaatcgg tatttgcgat ttgcggataa agtagatgga aaacggcgaa ccaattggcc
    25261 cttgaatgaa caatggggtc gttttattgg acggaatcgg aaagagattc aattaacttc
    25321 tgaaccgaaa ccgtatacca ttgaacgaac attaaattgg ctaggtagac aagtcgcccc
    25381 tacgtggaaa atggctaagg agttggatcg attgaatcaa acaacgtata tccaggatat
    25441 ggtacagaat gcgcgactct cggaccggca taaaaagatt ttggagcaac aaagtatggc
    25501 aatcgaaaac ttgattgtat gaaaagagga attttgatgg aaattgtaaa agatatgtta
    25561 ttggtacttg gtggcagtat gcttggtgtt accgtgatgt gcttgatgaa tgcaagtgca
    25621 gcagcggatt gagcaatgga aaagaaagga aagtgacaaa atgaatttcg gacaaaattt
//
LOCUS       DAZBWR010000028        25460 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000028 DAZBWR010000000
VERSION     DAZBWR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.028"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHGT5_001066"
     CDS             133..3660
                     /locus_tag="ACHGT5_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN7217067.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHGT5_001067"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHGT5_001067"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN7217068.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHGT5_001068"
     CDS             7448..7789
                     /locus_tag="ACHGT5_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN7217069.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHGT5_001069"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHGT5_001069"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN7217070.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHGT5_001070"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHGT5_001070"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN7217071.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHGT5_001071"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHGT5_001071"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN7217072.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHGT5_001072"
     CDS             13000..13737
                     /locus_tag="ACHGT5_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN7217073.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHGT5_001073"
     CDS             13751..14566
                     /locus_tag="ACHGT5_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN7217074.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHGT5_001074"
     CDS             14579..15226
                     /locus_tag="ACHGT5_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN7217075.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHGT5_001075"
     CDS             15242..15898
                     /locus_tag="ACHGT5_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN7217076.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHGT5_001076"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHGT5_001076"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN7217077.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHGT5_001077"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHGT5_001077"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN7217078.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHGT5_001078"
     CDS             18240..18758
                     /locus_tag="ACHGT5_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN7217079.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHGT5_001079"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHGT5_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN7217080.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACHGT5_001080"
     CDS             complement(19645..20565)
                     /locus_tag="ACHGT5_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN7217081.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACHGT5_001081"
     CDS             20691..21743
                     /locus_tag="ACHGT5_001081"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN7217082.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHGT5_001082"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHGT5_001082"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN7217083.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHGT5_001083"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHGT5_001083"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN7217084.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACHGT5_001084"
     CDS             complement(23383..24144)
                     /locus_tag="ACHGT5_001084"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN7217085.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACHGT5_001085"
     CDS             24333..25427
                     /locus_tag="ACHGT5_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN7217086.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8441 a         4420 c         5378 g         7221 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa
//
LOCUS       DAZBWR010000029        25132 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000029 DAZBWR010000000
VERSION     DAZBWR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.029"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(168..779)
                     /locus_tag="ACHGT5_001086"
     CDS             complement(168..779)
                     /locus_tag="ACHGT5_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN7217087.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(902..1027)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1109..1825)
                     /locus_tag="ACHGT5_001087"
     CDS             complement(1109..1825)
                     /locus_tag="ACHGT5_001087"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN7217088.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1831..2400)
                     /gene="scpB"
                     /locus_tag="ACHGT5_001088"
     CDS             complement(1831..2400)
                     /gene="scpB"
                     /locus_tag="ACHGT5_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN7217089.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2384..3154)
                     /locus_tag="ACHGT5_001089"
     CDS             complement(2384..3154)
                     /locus_tag="ACHGT5_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN7217090.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3151..3522)
                     /locus_tag="ACHGT5_001090"
     CDS             complement(3151..3522)
                     /locus_tag="ACHGT5_001090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7217091.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3541..4428)
                     /gene="xerD"
                     /locus_tag="ACHGT5_001091"
     CDS             complement(3541..4428)
                     /gene="xerD"
                     /locus_tag="ACHGT5_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN7217092.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4524..4997)
                     /locus_tag="ACHGT5_001092"
     CDS             complement(4524..4997)
                     /locus_tag="ACHGT5_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN7217093.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5102..5965)
                     /locus_tag="ACHGT5_001093"
     CDS             complement(5102..5965)
                     /locus_tag="ACHGT5_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN7217094.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6161..8260)
                     /locus_tag="ACHGT5_001094"
     CDS             complement(6161..8260)
                     /locus_tag="ACHGT5_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN7217095.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8480..9589)
                     /gene="rpoD"
                     /locus_tag="ACHGT5_001095"
     CDS             complement(8480..9589)
                     /gene="rpoD"
                     /locus_tag="ACHGT5_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN7217096.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9613..11454)
                     /gene="dnaG"
                     /locus_tag="ACHGT5_001096"
     CDS             complement(9613..11454)
                     /gene="dnaG"
                     /locus_tag="ACHGT5_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN7217097.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11636..13951)
                     /locus_tag="ACHGT5_001097"
     CDS             complement(11636..13951)
                     /locus_tag="ACHGT5_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN7217098.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14136..14996)
                     /locus_tag="ACHGT5_001098"
     CDS             complement(14136..14996)
                     /locus_tag="ACHGT5_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN7217099.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(15055..15687)
                     /locus_tag="ACHGT5_001099"
                     /pseudo
     CDS             complement(15055..15687)
                     /locus_tag="ACHGT5_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15854..16615
                     /locus_tag="ACHGT5_001100"
     CDS             15854..16615
                     /locus_tag="ACHGT5_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN7217100.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16641..19232
                     /gene="mprF"
                     /locus_tag="ACHGT5_001101"
     CDS             16641..19232
                     /gene="mprF"
                     /locus_tag="ACHGT5_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN7217101.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19420..20607)
                     /gene="tuf"
                     /locus_tag="ACHGT5_001102"
     CDS             complement(19420..20607)
                     /gene="tuf"
                     /locus_tag="ACHGT5_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN7217102.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20975..21331
                     /locus_tag="ACHGT5_001103"
     CDS             20975..21331
                     /locus_tag="ACHGT5_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN7217103.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21572..22576)
                     /locus_tag="ACHGT5_001104"
     CDS             complement(21572..22576)
                     /locus_tag="ACHGT5_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN7217104.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22742..23593
                     /locus_tag="ACHGT5_001105"
     CDS             22742..23593
                     /locus_tag="ACHGT5_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN7217105.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23610..23855
                     /locus_tag="ACHGT5_001106"
     CDS             23610..23855
                     /locus_tag="ACHGT5_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217106.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            24042..24746
                     /locus_tag="ACHGT5_001107"
     CDS             24042..24746
                     /locus_tag="ACHGT5_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217107.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7085 a         5200 c         4385 g         8462 t
ORIGIN      
        1 ttcgtctttt aaaaggagaa gaatttcaca tttgcaaaga attgattgat ttttatgcat
       61 aaatcaaaaa aatactcaaa acaaacaaat aaaacgttta cattattgca tgtcttgtat
      121 ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct
      181 gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc
      241 agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca
      301 atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac
      361 attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg
      421 atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta
      481 aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct
      541 accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct
      601 gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta
      661 aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact
      721 aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt
      781 tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa
      841 aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa
      901 gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt
      961 tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa
     1021 gacgaacctt atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca
     1081 acatctaaca cttataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt
     1141 cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt
     1201 atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt
     1261 gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca
     1321 ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg
     1381 tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat
     1441 ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca
     1501 agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg
     1561 gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt
     1621 taggtttgta caataaatag taaccgtatt cttcttgata aattgggact tcatccactt
     1681 ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt
     1741 ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg
     1801 caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa
     1861 tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc
     1921 ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg
     1981 ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg
     2041 taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg
     2101 tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc
     2161 ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct
     2221 ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc
     2281 tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt
     2341 actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat
     2401 gttcggtatc tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg
     2461 caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac
     2521 ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac
     2581 gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt
     2641 ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga
     2701 tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca
     2761 taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg
     2821 catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt
     2881 cccataattc ttcatcgtct gtgacgaatt cttggacagg aagcaatgtc ttgcttttga
     2941 ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt
     3001 tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata
     3061 tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat
     3121 ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca
     3181 ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc
     3241 ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga
     3301 aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat
     3361 gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt
     3421 atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc
     3481 catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga
     3541 ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt
     3601 ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc
     3661 agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc
     3721 tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga
     3781 aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa
     3841 tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat
     3901 gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc
     3961 tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa
     4021 gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc
     4081 tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg
     4141 ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag
     4201 acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag
     4261 aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag
     4321 aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc
     4381 ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct
     4441 catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag
     4501 caaagaaaaa gccgtctaga cactcagacg gactggtttc ttttttcttt ttcatagcat
     4561 tcctgacaga ttccatggaa cgttagccga tgatctttta ctaaaaaatg ataacgttct
     4621 tctactactt gctccacttc aagtaaaaga tcctcttcta cttcctcgat cgtgccgcag
     4681 ttcaagcata gtaaatgatg atggaaatgt ttggcgcctt cttttctcaa atcataacgg
     4741 gctaatccat cattaaaact tactttatcg acaattttca attctgtcaa gatttcaagg
     4801 gttcgataaa cagtagcaag tccaatttcc ggactctttt ttttcactaa aaagaagatt
     4861 tcttctgcag ataaatgctc tttctcattt tccaaaagca caagcagagt agcttctctt
     4921 tgcggtgtca gcttaaaccc cgcttcatgt agctgctgtt ttgttttttt catcgccgaa
     4981 atagcattca tgaccattta ctcaacccca acaatttata ataattacaa ataaaaaaag
     5041 atataacact atgttaatta aaattattat aagctagtta agaaaaacat gcaagggatt
     5101 ctcaattaga ttttttgact agttttgtca caccgttttc ttgtgtaatc aatccttgtt
     5161 tcaaaagatt acctaatgcg cgcttaaatg ctcctttgct gataccaaaa gctttcatga
     5221 tttcttctgg atccgattta tctgtaaagt tgatttgctg atctgcagaa cgttctaaaa
     5281 tggttaaaat catttgtgca tcatctgata tagcttcata tgctcttggc ttcaatgaaa
     5341 gattcagtac accgtctggt cggacaccaa tcactcgacc ttttatctgc tcgcctagac
     5401 gaggttcatt atatcgttca gaaggatgga taaacccaag atagaaatct tccgttaaaa
     5461 cgtacgtacc tgttaatttc aagcggtatg ctgttcctat cacatctttg tttttcattt
     5521 cttctgttcc atatttgctt aaagatttga atattttttc atctgctaaa ctgccccaaa
     5581 tacggtcttt tgcatcaact ctaagggaaa tcatcaagcg atcccctttt ttaggccata
     5641 gctctgtcat tgtcggcaat tcatcaagag atacgacaaa gtctttatct ggaagaccga
     5701 tatcgacaaa cgcacctaaa tcttttcgtg tagcagtaac gattccaaaa gcgtaatgac
     5761 cgatacggct tttaggaatt tgtgtagtga tccgattttc atgtttttga ttttggtaac
     5821 cgaatccttc aactgcttca ccgattttgt gttcaccttc tgatttgctt aagccaaagg
     5881 tttgtccgtt tttttggaca aagtagtttt tttcattttc atcaataata atacctgtaa
     5941 atatagttgc taataaatcg ttcatttttt tctccctcta tagtgaaagg ttttcttaat
     6001 acatttcttt gctatcataa cataatgtta tctacttgaa tagctgagcc ttggtttgga
     6061 aaggtgaaaa aaggagtatg acaacagttg tgtcatactc ccctttttcg aataaacggt
     6121 gttaaacggc aggctcacag cctctttcat ttgcctaaaa ttaattgctc ccatttcctc
     6181 cgaaccatcc agttttacag ttgatggtta cgatgtaaaa tggcaaagca ctttctccaa
     6241 tgcctttcat caatgcagat tttgttgaag tggcatatag attataatag ccttccccat
     6301 tttcgattct ggctttttct aatctgtaac ctccttcaac gatatatcca cgttggatac
     6361 atgtagcaat cactttgctt tggacacctg gtgcccacgt ccatgctgga ttagatgcat
     6421 cggcaatatc acttgctctg gcagatttga ttggctgatt cgctgagttt gaagaggaag
     6481 gggtcgagga tgccgcttct tgctgtgctg cttgtgcgac cgcagcttgt tgagcggctt
     6541 gagctgcagc tgctgcttga gcggcttgtt ttgcttgttc ttcagctgct tttttcgctt
     6601 cttctgcttt tttcttttcc tcttcttctt ttttctcttt atcagtgagc atcttatcta
     6661 cttgtttcaa ttgttcattc aactgagcag atagatctgt atttttcact ttttctactg
     6721 cttcttttgc cgtcttgtat tggtcgcgtg ttgctgaatc cactactttt ccgtctttaa
     6781 agactatagc tgttttttct ttagctgttt gcaattcagc atattgattt ttagcttctg
     6841 tttgtgcttg ttgaatcaat tttccaaaat ctgtgttatc cgtttcgatt gcttggatct
     6901 ctttatctgc tttcaacttg atgtcatctg ctagatgatc atcgacgatg gcaggtgcta
     6961 tgaaccactc attcactttt tggatatctc ggatcttaga ttggatatct tggtaagttg
     7021 tttctaactc tgaatatcct ttttcattct taagactgga taaatcattt tttagttttg
     7081 ataaatcttg atgaatcata tctgcacgaa taaattgatg gtgatcatct gtataaaagg
     7141 cagcaagttt ctcatgaagt tcttccattt gttgctgtct ttttgcttct gctgctactt
     7201 cggcttctac tttttgatga tggtcataat aagcccagct tcctccaccg agtagaagta
     7261 ctgctgctaa aacgagataa ggtacttttt ttgatttttt cttttgcgct ttatctggat
     7321 ctgttttttc taagtttgat tcttgatgtg ttttttcagt atctggcacg attgttattg
     7381 atttcgcttc tgcttcaaac agcttaggct ggttctcttc tttttctatt tttgcttcaa
     7441 gatcagtacc ctctttgctt acagattgat tttcagcatc ggattcacta tttatttcat
     7501 cgggctctgt tgactttttg tctgtgtttt cgagtatttc ttttttctct gcttccggca
     7561 gtttattcgt tttttccttt tctaattgtt tttcagtaaa tacttcagtt ttttctgtga
     7621 tatccgtttt tacgtctttt atttttactg tttctttacc tgtggcgttt tctctatcag
     7681 agtctttttc agtttcttgt tccttttctg gctctacttt gttttcctgt gcaatttttt
     7741 cactgatcat tttgagcagc tctttcgcgt gctcttcttt tgtatcttca cgatgtgctt
     7801 tgatataagc ggatagtatc gaattttctt cctcattatc gacgatttct ttcagttcag
     7861 ctactcttct tttttgtttt gattcaagcg tttcttcttg attctttcct gattcttccg
     7921 aagtctcatg aacagttgct ttcttctgtt catttttctc tatattagtt acttgatcgt
     7981 gagcaggtgg ttcttcgtct tctggttctg ctttttcgaa taattccatt acagattcta
     8041 gaggtaaatc tttcaattcc gaccattcga ttggatcgtt taattctgta tcaggaaaga
     8101 ctgcatcttc agttgattta tcctgcatag ctgcatctgt atttgtttct tcaattgatt
     8161 cttttatttg ctgatcttct gcagttattt gatgaccgca gtctggacag atagtattct
     8221 gctccatttt tttcgatccg caatttggac attttttcat cacttagcga tcggctcctt
     8281 tactaggttt acgctaccat ttaatctatc atattctata cgtcacgcca atagcttatt
     8341 tttaggattt acttaaaaat aatgaatgaa tttccttcct atagaaaaaa aagcttgaga
     8401 ctgcgtagtc tcaagctttt tttcttttaa aataaaccaa acagttttac tgatttctct
     8461 tctggtcaat agtccacgct tattctaaga aatcttttaa ttgttttgaa cgagaaggat
     8521 ggcgcaattt tcttaatgct ttagcctcga tctgacgaat acgttcccga gtaacaccaa
     8581 atactttccc tacttcttcc aatgttctcg tccggccatc atctaaacca aaacgtaagc
     8641 gaaggacatt ctcttcccga tcagtcaatg tatctagtac atcttccaat tgttctttta
     8701 gtaactcata agctgcgtgt tctgctggac ttgttgcttc ctggtcttct atgaaatcac
     8761 ctaagtgaga gtcatcttct tcaccgattg gcgtttcaag ggaaacaggt tcttgcgcga
     8821 ttttcaagat ttctcggact ttttctgttg gaagatccat ttccgcaccg atttcttctg
     8881 gcgtaggttc tcttccaagg tcttgcagca attgacgttg gatacgtatc aatttattaa
     8941 ttgtttcaac catatgaact ggaatacgga tcgtacgtgc ttggtcagcg attgctcgag
     9001 tgatggcttg acgaatccac catgtagcat aggttgaaaa cttaaatcct ttgcggtagt
     9061 caaatttttc aactgccttc atcagtccca tgtttccttc ttggatcaag tcaaggaact
     9121 gcatgcctcg tcctacataa cgtttagcga tacttactac taaacgaagg tttgcttctg
     9181 ccagacgctg tttcgcttct tggtcgcctt cttcgatttt caatgctaac gctacttctt
     9241 cttccgcagt taaaagggag acacggccaa tttctttcag atacatacga accgggtcat
     9301 tgatttttac accggttggc gcagaaagat cttctgtttt tgcttgttca gcttttttct
     9361 cagcactctt caagctgtga attgatggat caccattttc atccaccaca ctgatacctg
     9421 cgtcttcaat cttttggatc aatttgtcca tttcatctgc attcaaagtg aatggtgtag
     9481 caagctgatt ggataaatca tcatatacaa cttgtcgttt gggtttgttt tctttgatga
     9541 aagctgctac tgctgcttca tacgtttttt tatctgttcc atttgccata aaagaaggcc
     9601 ccccttgagt agctatttgg cttgttttaa ctgtttcgtt aaattgatga tttcaatcgt
     9661 caattcaagc tcaagctgtt tgtttccagt tcgtctggct tcttgctgca tttgtttttt
     9721 taatgttatt tcatcagcta agccagattt gctgatcaca tctagcaagt cttggatttc
     9781 tcgtggcgaa ctttcttctg ccaccggtaa atattcgatt tccaccacca accgccttaa
     9841 attttcatct tgcaaaaagt ctaaaaactc tgcaaggttg aattcataat gttgtgagac
     9901 atatgcatcg aataaaaaat aaacttctgc atatgcatca tgaataaact gaaattctcg
     9961 ttcttttaaa atattacgga cagaaacttc tttaaatgca cgatataata gaatctgctc
    10021 tgccttttct acttgcgtca aaggtctctt tttccgtgca gtttcttgtc tcgtaacctg
    10081 agtgtgttga ctttcctgag gttcttgacg attttgctga tttgtccttc gaagatcgcg
    10141 cagttgtttt tgtatcgtct ctctgccgat ctgaaactct gttgacagtt gagatagata
    10201 catatcttgt tcgattggtg aaacaactcg gctaagttca actagtaatt cttcgaggta
    10261 ttcgatcttg tctttttcat tatctaaatt tttcccttgt tttaaataac gtgatttaaa
    10321 agtaaagact gtttcgcgcc catgagccac taattcttgg aaagatgctt ctccatactt
    10381 acggagatat tcatcaggat caagcttttc tggaacgact acgacagaaa gtgctaatcg
    10441 actgttttgc cgaagcaatt caatccctcg attggttgct tcaaaacctg cgttgtctcc
    10501 gtcatagcaa aagaccaatt ctttcgtcag cttttccatt tgttggatct gttgagctgt
    10561 tagacttgtc cccatagaag ctaatccagt tttgatacca gcctgccatg cagcaatcac
    10621 atccataaac ccttcgaaca acaaaatttc tccgtttttc cggatatcgc ttcttgcttt
    10681 atcaagatta aataaaactt gtcttttatt gaacagttct gtttctggac tattcaagta
    10741 tttgggctga tctttttcct gccctttttc aggtgcaagc cagcgtcctg aaaaaccgat
    10801 cgtctggcct tgagaattac gaagtggaaa catcactctt tgataaaaac gatcagacat
    10861 acggccatca tcatgcgtga caaacaatcc agagcgttct aacaattcat ttgaaattga
    10921 ttcattcttg aatacttgct gaagaaaaga acgctcatta ggtgcaaaac ctatgttgaa
    10981 ttcttcaatt aattctaaag acaacccacg actttgcaaa tattctaaag ctggctgtcc
    11041 agcttttgta tgcaatagca tgtgatgata gatttccgct gctttttcat gagcagcaat
    11101 cagtttcccc gtagcagatt caggattttg gacagctata gtacgctgac gccattgatc
    11161 ttctaacggt atctgttcga agtctgccac ttttaccact gcttctggaa aacttagacc
    11221 ttctagttct tgaagaaaag taaacacgtt tccgccttta ccacaaccaa agcaatgaaa
    11281 aatctgcttg tcttctgcaa cagaaaaaga cggtgtacgt tcttcatgaa aaggacacag
    11341 ccccaagtaa tttttaccag actttttcaa ctggacatat tgtccaatca cctcaacgat
    11401 attcgtttga ctgcgtactg tttcgataac ctcttgagga atccgttgag ccattttttc
    11461 accccctgct ctattgatat cttttgggtt taacagaaga tatttcagga gaataaccgg
    11521 taaaaaatcg cactaactga aatcagtgcg attttaaagg acacaattat acatgtttga
    11581 ttgtatcata tttcattgct ttttcaagta taacacattt ctatctcatt aagcatcaat
    11641 ttgctactct tttcagtaac tgattttgaa tatcagcctt ccagtacaat actcgaagaa
    11701 caatatttac gagcatgaat acaaagaagt atcctacgct ctctcccaac cctaagttta
    11761 cttccaaaat tccatggaac agcattgcag ctacatatat ccctacgatc gtcgttactg
    11821 ctaggaagat gcgcaatggg ttgaagggat aacaagcttt tacaactgcc atgcaactga
    11881 tcccaatcag caagtaatac atcaaagtag tagtttcagc atttccccat gcgaatgttt
    11941 ttccaagtag atacaccgca atgatattca ctactacaag caaggcattt ggtaaagcat
    12001 taataagtgc tgtaggcaaa taacggtaag tgatttttct tttatcttgt tcaaaagata
    12061 aaaagaagga aggataccct tcaatcgcta aatcaatcag cgtgatttgg atagggataa
    12121 acggaaatcc aattgctgta acggcacata taatcgataa aataaatgaa taaatcgttt
    12181 tgataaaaaa gatgcctgag actttcgtca cattgttgac cactcggcgt ccttcaaaca
    12241 ggacttctgg caatgtagta aaatcagagt ccaacaatac cagattcgca atctgacgtg
    12301 ttgctccatc accttctgcc atagcaatgc tgcaatctgc ttctcttaac gccaaaacat
    12361 cattcacacc gtcgcctgtc attgcaaccg ttctgccgct ttctttcaat tcatttacca
    12421 gcaatttctt ttgctgcgga ctcactcgtc caaaaaccgt atagcgatga gcagcttctc
    12481 gcacttccat ttcttcagta atttgagaaa gatcgacata cgcatcataa ttcggtaatc
    12541 ctgcacgtct agcgatattg gagacagtaa aaggattgtc tccagaaatc actttcagat
    12601 ccacaccttc ctcatgaaga taagctagtg tttcttgagc gttttttcgg attggatcat
    12661 cgatttcaaa cagagcaacc ggctcaacaa atgctggttt ttcttcgttc aattgctgat
    12721 ttggagcgat ccccaacata agtacgcgat agccattcgc ttgggctgtt tttagactgt
    12781 ccggaagccg atcttcttcc accaaacgtt caggcgctcc tacgtacacg actcccagtt
    12841 ctggaaattc catcgcgccc catttgcgat cagaagaaaa tgcgactttg tttgtcacat
    12901 cataccgatt agaaagctca aaataatctc tcatagcccg catagtgatg ttgttatcac
    12961 tgctttctgc cagatacgtc cccataattt ctgggatggc tttctcgtac agcggatgta
    13021 gtgcttctac ccgctggata ctcattttcc cttcagtaat ggtccctgtt ttatccaagc
    13081 ataatgtatc gacatgggcc agcgtttcaa ttgaatacat gtcttgaaca aggattcgtt
    13141 tttttgccag tttggttacc cctgtcgtta atgcaatact tattagcaat accaaaccct
    13201 ttggaagcat gcctaacaaa gctgctgcag atgcaataac agaaattttg atagaagcat
    13261 cgcggatgaa cagtgcttcc aaaaatagta tgatccccaa aggaataatc acgaaactag
    13321 tgaatttgga tactttgcga atcgaatcta caagttcaga acgaatagct ttgtgagtct
    13381 ttgcctcgct agtcaagcgg acagcataat tatctgcacc tactcggata acttcagcca
    13441 tgacagaacc gcttgtcaaa aagcttccag aaagcaattc agctccagtt tctttgacaa
    13501 tcaaatcaga ttcgcctgtc aaaagagctt catttgcttc tgcccgtcca gatataacct
    13561 gtaaatcggc tggtatctgt tctcctgctc cgatttttac caaatctcct aataccagct
    13621 ctttagtaga aatcgattgt tctttaccgt cccggatcac tagtacttct gattgggaaa
    13681 caatcgataa tttccgtaca agatttcttg catgtatttc ttgataaatt ccaataacga
    13741 tattgacgac aataattgct aaatacgcca tgttggaata cgcacctaca aataacagac
    13801 aaatcccaat accaaagttc agaaaattga ataacgtcaa tatattatcg ctgaatattt
    13861 gttttgtact ttttgatgca tcttcctggt agtcattttg ctggccagaa gccattttct
    13921 gaactacctc ttgctgtgta agtccattca ttttcttttt ctccattttc cgccaacttt
    13981 caaaaataat cacgtgttta agtctagcgt gttccaagat taaaaacaac ttgtttttat
    14041 aaaatttata gaaaaacaca cgaatgattt tacaaaaaag atgccaacct ttttgtttga
    14101 atcacccgtg tgctgttttt attatcaatc tatctttact tatattgttc ctccaaacgg
    14161 ctcatccacc aacctagtgc tgtaccggct ataaaaagaa caggacaagc tagtacccca
    14221 gcaactccca atcccgcgta atcggtagga atgatcataa tcgttgaagc aaaaactaca
    14281 cctaaaatga aatggaatag cttgctatat gcctttgaga aaatgtaatc catgattttt
    14341 gacaatccaa gaacacaaag aatcccacca attccgatcg gcacaatcac ttcaagtcgt
    14401 acttctttaa atccgtcagc catcgcttta tacaatccca tatacaccaa aaaattagaa
    14461 gggctcaatc ctgggacgat catacccaaa ccaatcaatc cgccggcaat cagccatgtt
    14521 ccaaaattct gatcgacttg cccgtcaaat aattgagcgc cgtataataa aaacaaataa
    14581 gccagaacaa aagtaacaac catgatgatc acatcagaag tatctcttcc ttttttccct
    14641 gcttctttcc ataacgcagg aacagttcca gcgatacatc cgacaaaaaa ccataatatc
    14701 gttgtttcat acgcacctaa taaaaaactg actaaaaaag aaagtagaaa gatgcctgtc
    14761 actccgccta gaccaacagg cagaaaaaac aaaacatttt ctttgaaatt ttttgtaagg
    14821 tgggctaaaa aagaaatcag ccgctcatac atgccaaaga cagctgctaa tgcaccaccg
    14881 ctgactcctg gcaagataaa tcctgaaccg ataaacattc ccttcacaaa ccgtaacatc
    14941 cagtctttgg ttgtgtaccc cacttcttga ttttccattt tttgcctttt ttccactatt
    15001 tgcgtactcc ttctttcatt taattttatt catgattgaa catttcctgc tattttactt
    15061 aaagactttt tcataggtct ctattatttt ttcattcatt ttgacaatat caggataaaa
    15121 catgttacct ccattatgat agagatagcc gccattgtaa agcaaggact ggatccgcca
    15181 atatcggtat tgtttccctt cctcatttcc taaaagtggg ctcagcacat ctttagaata
    15241 cgcttctgct aattctactg tgttttctcc gccatgattt tttacatagg caatataatc
    15301 tttcccaaaa ttatatgctt ggacagctgt ccataaatcg catccttgtt tctttgcttc
    15361 tactatcatt tccgccaaat atttgacacc ttgatcaata ctgtcagctg gattatcaat
    15421 gatctcagct tctccatgca gactttccga gctctgcatg agatctgtcc cttttccttt
    15481 tgattcggtc aatattatcg ccttgatcaa attctcgtag ttttccatat gataattttt
    15541 tgcagctgtt gttatttcta tttgatagct ttccactcgt tttaccgaac gataagttct
    15601 tcctcataaa aacaacaccg caccaataac cgtcagaatg atcatgtaaa aaagaatacg
    15661 gataatagat aatcgttttc gcttcatttc atcacctagt tattttgaca tcttttttta
    15721 tcttactatt tctttatctt cttggctatt tcttccttga tttctgtgta cgccttaaga
    15781 aaaaatgaat tttcctgtat tttttgacat cttacgaaaa tgatcgttgt ttttcagtaa
    15841 aaacaattgc ccaatgactg atataatcgt tatactttac acagtaggtg attatgtgaa
    15901 aaaagaaaag aaatatgcaa aaatgccaga tgggagcgaa atttactatg aaaagagcgg
    15961 tcaaggcttc cctctctttt tactgcatgg caatgacggc agcggccgct ttttttcaga
    16021 acaggtccct gtactcgaac gctactatac cgtgtatcta gtagatagcc gaggacatgg
    16081 tcgttcgaca aacgaagcat ctatgttgaa ttttcagtta atggccgagg atctgaatac
    16141 gatcatgttg ctggaaaaaa tcgatcaggc agattttctt ggatttagtg atggagcaaa
    16201 tctagcactg gtatttgcca gcagcttccc taaaaaagtt catcgattga ttttaaattc
    16261 tggaaacacg cttgttaaag gcgttcgttt ttctgccaga gtcatcagta atattcatta
    16321 tgcatgggta tggttactct ctttatttcg cccaagtctt cgcaaaaatc ttttagttat
    16381 caaacttttg ctgcatgaca tcgggcttac agaaaatgat ttaaaaaaaa tcaactcccc
    16441 cactttgatc atcgtgggaa aaaaagatgt aatcaagcta aaacattctt tgtatatagc
    16501 taaaactata ccaaaagctt cctttgtgct tgtgaaagaa caaggacatg aattagctag
    16561 aaaagatcct gaacgcttca atcgagaagt tcttcaattt ctttctgaaa cataactatt
    16621 aggaatagaa ggtgatttat ttgttaaaaa ataccataca atggctgaaa aaccacctag
    16681 gactgttcaa aacaatcttt ttgatttctg tcattgtcat tattgtcggt gaattgatgt
    16741 ccatcggtaa aactctatct atccagcaat taggagaaac gtttgccact atacctgtat
    16801 ggaaaagcgg actaatgcta ttgattggtc ttatctctgt gttacctatg gttggttatg
    16861 atattatttt gaatcggatg cttgaacaaa agcagaatcc tcgttacctg tttgagacaa
    16921 gctggctgat caatacgatc aacaatattg ccggtttcgg cgggtttgtt agtgtggggc
    16981 ttcgctctga actatatgga caaaagaaag agagcagaaa cgtgatccaa gcattatcga
    17041 aagttttctt gtttttgatg gctggacttt ctatttacag tctgctttct tttttacttg
    17101 tagtcttcac gccagttgct ccttttttga agcaatattg gatatggttg atcggcggcg
    17161 gtctatattt tcctgtcgtc tttttattta ctactttcaa aaagaaagga ttccttggtg
    17221 gtacctcact taaaactcaa ggacagctat tgctagtttc attgttggaa tggaccggtg
    17281 tcctgtttag tttcctttct gttggttatt taatggaaat ccggattgat ctgtggcaaa
    17341 caattccatt attcattgct gcttcagtga taggaatcgt gtctatgatt ccaggagaaa
    17401 ttgggagttt cgatgtaatg atgattatcg ggttgtccgc tattggtgtg ccaagagaaa
    17461 tagtcgttgt gtggatcttg ctctatcgcc tgttctacta catcattccg tttttgattg
    17521 gaatcgtctt tttcttcaaa aatatcggtt ccacttttga tcagcgttat tctggtatcc
    17581 ctaaacaatt agcaacagaa attgcgcata aaatcgtggt tgtcttatta tacttttctg
    17641 gaatcatgct agtactttct gccactatcc cgcaagcttt tacagagttt cgctggcttc
    17701 atagtttgaa cccattgaaa tttcatttta ttatccaatt tcctagtata ctattgggat
    17761 ttcttttaat cattatgggc agagggatcg cagcacgtgt gaaacgagca tatttgccta
    17821 caatcttctt gattgttcta gctttacttt atgttttatt aagtgatttt agtttcacac
    17881 cggtggtttt cttgtctatt ctgcttctga tcattcttgc ttctaaaaac gaattatttc
    17941 gggaacaatt gatctattca tgggagtggc gtacgatcga tggaatcatt attggtgcac
    18001 tgtccttgct ttacatcgtt attggggtat acaatctgcc ggatttccct catcggcgtc
    18061 accattttat ttcctttttc ttatttcctt ctgaaaagat ttggttttcg ggattgttgg
    18121 ccatcatcgc tgttagtttc atgatcgttc tatttgttca tttccttcaa ggagaaaaga
    18181 aacagattgg cgaagccttt aatgaagaaa aggccctgaa gatccttaca acttatggag
    18241 gaaactccga tagtcaattg atcttcttaa aagataagcg gatgtttgcc tatgaaaaag
    18301 acggtgaacc aactgttctt ctccaattcg cttgcttcaa taataaatgt attgtaatgg
    18361 gtgatccttc tggtaagaaa gaagattttc cagaagcaat cgaagctttt atcgaagaaa
    18421 cagatcgact atgctattta ccagttttct acgaaacaag cgaagagatt gttatgattt
    18481 tgcatgaatt cggctacgat ttcattaaga tgggggaaga agcttatgta gacctgaata
    18541 gttttacaac ttcagggaaa aaaatgaagg gcactcgtgc tgtcttgaat cggatcgaac
    18601 gagaaggatt tacatttgat gtcttacaac caccattttc tactgagcaa atgagtactt
    18661 tcaaaaatat ctctgataat tggttgggtt cacgaaagga aaaagggttt tctttaggct
    18721 ttttctctga agattatcta caaagagcgc ctattgcagt tgtcaaaaat acccaagaag
    18781 aaattgtcgc ttttgcaacg atcatgccta cttacacgaa caataaagta ggaacgattg
    18841 atctgatgcg atatgattct aaaaaagcac cttctggcag tatggatttc ttgttcttgc
    18901 atttgtttgc ctatatgaaa gaagaacata tccaatggtt caatttaggg atggcccctc
    18961 tttcaaatgt cggaacttcc agaaagagtt ttttacagga aaggattgca tacttggttt
    19021 acgagtttgg ttctcatttt tattctttcc atggattaag agaatataaa agcaagtacg
    19081 ctacaaactg ggtctcacgt tatactctat attctagaga tagttggatt gcatatgtta
    19141 tgattgcttt gctgattatt gataatgcgc cagttgagca gacacaaggt agaataagtg
    19201 gtttgaaaaa atggatacga agaaagtatt aaaaactgca taccgataga tgaacactct
    19261 catgatgaat aaaagagact gaagtaaaac gaaagttcat gcttctacaa tctcttctta
    19321 tacaattgaa gaacaaagag cggtccaaac agaaatcctg tttggaccgc tcttttattt
    19381 taaacatttt tcattaccaa aatattcgag tcgcgtttct tatgcttcga tctctgtaac
    19441 gatacctgaa cctactgtcc gtccgccttc acgaattgag aatgtcgttc cgttttcaac
    19501 agcgattgga tggatcaagt caacatcaat cgtcacgttg tcaccaggca tgaccatttc
    19561 tgtatcttct ggcaatgtga ttgttcctgt aacatcagtt gtgcggaagt agaactgtgg
    19621 acgatagttg ttgaagaatg gagtatgacg tccaccttct tcttttgtca acacgtacac
    19681 ttctgctttg aattttgtat gtggagtaat ggaacctggt ttagcaagta cttgtccacg
    19741 ttcgatatcg tcacgttgga tcccccgtaa caacacgcct acgttatccc cagcttcccc
    19801 gtaatctaac gttttacgga acatttctac acctgttaca actgcttttt gtgtttcagg
    19861 tttgatccct acgatctcta cctcatcacc gacacgaaca gcaccacgat caatacgacc
    19921 tgaggcaaca gttcctcgac ctgtaattga gaagacgtct tccactggca atagtaatgg
    19981 tttatctgta tcacgttctg gtgttgggat atattcatct actgtatcca tcaattccat
    20041 aatagcagct tcagcatctg gatcgccttg caatgctttt aatgcggaac ctttgatcac
    20101 aggagtgtca tcgcctggga aaccatattc gctcaataac tcgcgaactt ccatttctac
    20161 caaatcgatc aattcttcat catcgactaa atcaactttg ttcaagaaaa caatcaggta
    20221 ttttacacca acttggcgtg acaataaaat gtgttcacgt gtttgaggca tcggaccgtc
    20281 agttgcagaa acaactaaga ttgcaccatc catttgtgct gcccctgtaa tcatgttttt
    20341 cacatagtcc gcgtgtcctg gtgcatcaat atgcgcgtag tggcgttttt ctgtttcata
    20401 ttctacgtgt gcggtattga tcgtaatccc acgttcacgt tcttctggag ctgcatcaat
    20461 actagcatag tcttgaggat ttgctaggcc ttttttgcct aatactgtag tgatcgcagc
    20521 agttaaagta gttttcccat ggtcaacgtg tccaatcgta ccaatgttaa cgtgtggttt
    20581 acttctgtca taatgttctt ttgccataat ttacaaacct cctaaataat atagatcact
    20641 gaaaaatttc agctctcacc tttttattat agtctttttt tgactaaatc aaaaagaata
    20701 cgtccgactt actttttgta agattcgaac taagcttatt atacgtcaat attggtcaaa
    20761 gtcaactaag agcactcgtc tgtcttatac gcgaaaaaag taccttttat aaataaaaaa
    20821 atgtgttttt tgagaaaaac ttcaagatag tccttaactt tcttttgaag caataagaac
    20881 aaaatgagga aaagtttaaa attcagctgt tgccaacaac tttcaataga cgaaatcttc
    20941 tatttcgtta ataataagga aagaggtgaa gtacatgaaa aaattaattg gcgtacttgt
    21001 cgttttaggc atatttctcg gcggaagttt tgcagcttat cattattttt acggcggcga
    21061 agcttattat acaaaaatca cgacgaatgg cgaaatgtca gctacccaag cagataacgg
    21121 agagaaattc acgacttata cctacaagca agcggcttat aataaagatg gtgaaaaaaa
    21181 acaggtgacg cttcgcgaag aaagagaaca tccattaaag atgaatgcgt atcttaaact
    21241 caaagtcaat cctcgtaaag gtgtattaag ctgggaagaa ataaaggctt ctgaagtgcc
    21301 taaaaatgcg gcagaaaaaa tcgataatta gtaaatggtc ttaaaatttt attagataaa
    21361 aagaggctgg gacagaagcg tttaactcca agaaataaga aggagttgct tctgcttccg
    21421 ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc tcacgctctt
    21481 tttttgttat attcgtcttt tccgatcctt ctcttgacac aattcattta actgatcact
    21541 ggtacttgaa cacctaatat tctagtcatt tttataaacg attcaatact tcatccacat
    21601 ttttgatcat cacggatatc gtaccatccg gattattcac aaattcaatc aaatctgggt
    21661 tgcgatatac atctactggt acgatcagtt cgatcccgtt tgatagcttg aatttttgtt
    21721 ttccaaattt tttctcagaa atttcttgta cttcttttac aggaggtgct tctgggacaa
    21781 atccttgctc ttgtacttct tctttaaaag ccatttttgc cgtgatgttt tccttgaaga
    21841 ctttttccgc aatcatctca tgatccaacc gacctttttc ttctatcgta tcatagacag
    21901 cttctttcaa atcagccatg atatcaaatt cttctgtctc aaattttttt ccaagatgct
    21961 tggctacttt ttggatcact tttacatttt cttctaaaga aggtgcagga acactttcga
    22021 tcactcttcc agaaaaataa agctcttttt tgccagaaaa ctcatatttt ttttcaatta
    22081 attcgtaggt ccaatcagtc aaattcacag cgaatgcttc atctgctttt tgtgttttcg
    22141 cccctagaat tgctcgattc aaaatcaagc ggttgtcgat ccctgcttcg tctgcttcaa
    22201 caaagtgagt gtaagcctca cgataattga ctttcaataa agataagtac atcactgtat
    22261 ctaattcata caagacaaca aatacatctg cactaggtgc ttcttcactt tcttggtaga
    22321 tatcgaacca ccgctgaacg attgcttcac ttgcttccac aaaattttct tgagcgattt
    22381 taagtgattc aacgatcatg ctttcttctg ctagttgtcc agtttttgtt tgagcagaag
    22441 agattttttg gatcttcttt tgaagaaaat cacgaatata ttcttttgtc agatccagct
    22501 ctttttgtga aaagactgga gaaccagttt ctctgtcaat aatatgcaga attgcttttt
    22561 ttaaatagat atccattttc tcattccctt ctgtcttagt ttacggaaaa aaaatcaaaa
    22621 ataccagaat caaaatcatt ttcttttaaa caaaaatttc aagagtagaa acaaaatgga
    22681 ttcttttttt cgaaaaatgc gctacactta tagttgaacg aataaaaaag gagctgaaag
    22741 tatgaattca ttaacaagca cttatcgtct atcaaatggt tacgaaatcc cggtagtagg
    22801 attcggcaca tggcaaacac ctgatgggga tgtcgctgtt tcctctgtta aagaagcgct
    22861 ggctgcaggt tatcgccata tcgatactgc ccaaggatac aagaatgaag aaagcgtcgg
    22921 ccaagcgatt aaggaaagtg gaattcctag agaagagata ttcttgacaa caaaattatg
    22981 gaatgcaaac cacagctatg aacttgtgat gtcttctttt gaagaatcat tgaagaagct
    23041 ccaaacagat tatttagatc tgtttttgat ccactggcca aaccctgttg cttttcgtga
    23101 taattgggaa caagcaaatg cagatacatg gcgagcattt gaagaactgt atgaagcagg
    23161 taaaatcaaa gcaatcggtg tgagcaattt cttaccccac catctagata cactagcaaa
    23221 aacagcaaaa attatgccga tggtcaatca agttttcctt gcaccaggcg aattacagac
    23281 aactgtagtt gagtatgcaa aaaaacatga tatgatcctc gaagcatata gtccattagg
    23341 cactggtaaa atcttcgatg tgcctgaaat gaaacaaatc gctgaagcac atgacaagac
    23401 gattgcacaa gttgctttgc gttggtcgct gcaacacgaa ttccttcctc ttcctaagtc
    23461 agtgacacca agcagaatca aagaaaatac tgaattattt gattttgaat taacagaaga
    23521 agaaatgaaa caaatcgatc agttagatgg tgtagtcggc aaagcaaaag acccagatac
    23581 tacccaattt taagtcgaaa ggtgatttta tggatccaat cgaacgcttg aattctctct
    23641 cggaagaagt tacccagact tttcattccg attttgtctt tttgatcgat gcagagaaaa
    23701 tccaacattt tcctgcaaga aactggacac atgatcaaat catcgaagaa ctgaaaaaac
    23761 ggttcgatca ttcgctaatg gtcaaaccct ggcacgaaca tgaagtcatt tattcacctg
    23821 aactacctgt ttttgccttg ataccgaaaa aatagaaaga atggaacgga ttatgtatat
    23881 atacataatc cgttccattt tttatttttc tttcaaaaaa cgggaaaata cgcaaatttt
    23941 tctgttgtaa tttgcgtata cgcaagtttt tttgcattac tccttttcat ttggctgttt
    24001 ttttcttata atcgaaaggg tatgagcgaa ggagagaaca tatgaagaaa aaacagaaac
    24061 aaaaattatt aaatcaattc cgtccttcat tggatggtgt aagaggacaa ttattccgtt
    24121 ctctagaaga agagagcttg cgacgttatg gtttgccgtt acaagtcatg cttgatccca
    24181 aaaaccgtca aatcttaacg atcggattag ctgggaaaac aaatgaagat acacggatca
    24241 acgtgccaat cgatgacaac ttcaatactg tattaaagag aattcgttca ggagaaaacg
    24301 ggattttcga acgattccgt gacaatcttc ttattgaaat cgtgtcttac tggaatgatc
    24361 aacgattgaa aaacgatgaa ccaactgcgc aagctgttgc gacaccagtt acagaagaaa
    24421 cagtcagtgt gccagaagaa aaaacggaaa aaccaaaagc ggacacaact gaaaatacac
    24481 gctcaactga tacgaatgcc gctttaagtt ttgctgattt ttcaaaagcc gttgctgagt
    24541 atcctaaatt ttacacagaa caaaatgaac atgaagtagt tattaatgaa aaaaatgctg
    24601 atgaacctcg taaattagca actatttcta tgactactga aaatgagttc gttatcgaaa
    24661 aagcactcga acggaaatac aaagtcaaat tgactttgat tcctttgatc gaacaatttg
    24721 cagcgacagc gattactgat cgataaaaat tctatttgtc ataaaaggct gggatttaaa
    24781 atcccagcct ttttagtcat ataataattt ctacagcaag taaaaagaaa ttccttctaa
    24841 catcaggctc aaatatcagc ttgacctgtc caacaaatca cctatgatcc tacaatgtta
    24901 ggcgacttct cttctacctg aagatatctt ggagttgcct gatttcttaa gatgcctaac
    24961 cttaactata aaaaattcta ataataaaca tattttctga tagagaaaaa agcggttcta
    25021 taatattttg tgttggtgga aaatcgaaag cgattttcta ctaacacttt tttcttcgtt
    25081 tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacgt ca
//
LOCUS       DAZBWR010000030        24255 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000030 DAZBWR010000000
VERSION     DAZBWR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.030"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACHGT5_001108"
     CDS             complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACHGT5_001108"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN7217108.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACHGT5_001109"
     CDS             complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACHGT5_001109"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN7217109.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACHGT5_001110"
     CDS             complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACHGT5_001110"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN7217110.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3371..4678)
                     /locus_tag="ACHGT5_001111"
     CDS             complement(3371..4678)
                     /locus_tag="ACHGT5_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN7217111.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4681..5262)
                     /locus_tag="ACHGT5_001112"
     CDS             complement(4681..5262)
                     /locus_tag="ACHGT5_001112"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN7217112.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5378..5475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5834..7126)
                     /locus_tag="ACHGT5_001113"
     CDS             complement(5834..7126)
                     /locus_tag="ACHGT5_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN7217113.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7123..7746)
                     /locus_tag="ACHGT5_001114"
     CDS             complement(7123..7746)
                     /locus_tag="ACHGT5_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217114.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7749..8585)
                     /locus_tag="ACHGT5_001115"
     CDS             complement(7749..8585)
                     /locus_tag="ACHGT5_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217115.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8602..9489)
                     /locus_tag="ACHGT5_001116"
     CDS             complement(8602..9489)
                     /locus_tag="ACHGT5_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217116.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9505..10788)
                     /locus_tag="ACHGT5_001117"
     CDS             complement(9505..10788)
                     /locus_tag="ACHGT5_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217117.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11471..12154)
                     /locus_tag="ACHGT5_001118"
     CDS             complement(11471..12154)
                     /locus_tag="ACHGT5_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN7217118.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12172..12789)
                     /locus_tag="ACHGT5_001119"
     CDS             complement(12172..12789)
                     /locus_tag="ACHGT5_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN7217119.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12799..13623)
                     /locus_tag="ACHGT5_001120"
     CDS             complement(12799..13623)
                     /locus_tag="ACHGT5_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN7217120.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13616..14176)
                     /locus_tag="ACHGT5_001121"
     CDS             complement(13616..14176)
                     /locus_tag="ACHGT5_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217121.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14350..15306
                     /locus_tag="ACHGT5_001122"
     CDS             14350..15306
                     /locus_tag="ACHGT5_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN7217122.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15611..16567)
                     /locus_tag="ACHGT5_001123"
     CDS             complement(15611..16567)
                     /locus_tag="ACHGT5_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN7217123.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16671..17273
                     /locus_tag="ACHGT5_001124"
     CDS             16671..17273
                     /locus_tag="ACHGT5_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN7217124.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17361..18860)
                     /locus_tag="ACHGT5_001125"
     CDS             complement(17361..18860)
                     /locus_tag="ACHGT5_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN7217125.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18876..19400)
                     /locus_tag="ACHGT5_001126"
     CDS             complement(18876..19400)
                     /locus_tag="ACHGT5_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN7217126.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19698..20447
                     /locus_tag="ACHGT5_001127"
     CDS             19698..20447
                     /locus_tag="ACHGT5_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN7217127.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20438..21223
                     /locus_tag="ACHGT5_001128"
     CDS             20438..21223
                     /locus_tag="ACHGT5_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7217128.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21192..21908
                     /locus_tag="ACHGT5_001129"
     CDS             21192..21908
                     /locus_tag="ACHGT5_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN7217129.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22010..23029)
                     /locus_tag="ACHGT5_001130"
     CDS             complement(22010..23029)
                     /locus_tag="ACHGT5_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN7217130.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23048..24049)
                     /locus_tag="ACHGT5_001131"
     CDS             complement(23048..24049)
                     /locus_tag="ACHGT5_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN7217131.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7620 t
ORIGIN      
        1 ttgctattct attttaattg aattttgcaa ctcactcttc tttattccaa acctactcgt
       61 ttgaatatca cgtcaacgtt tttcaaatga tgatgataat caaaggcatc gtctatttct
      121 tctttagata ataccgatgt gatggttgga tcttcttcta acagcgggcg gaactgcact
      181 tggttgtccc aagcgtacgc agtttttggc tgaacaagat cataagcttc ttctcttgtc
      241 attcccttat cgatcaattt cagcataacg cgctggctat aaatcaaacc aaatgttgca
      301 gacatattcc gcttcatgtt ttcaggaaag accgttagat ttttaacgat attgccaaaa
      361 cgatttaaca tgtaattcaa taaaatcgtg gtatcaggca agatgattcg ttctgctgaa
      421 gaatgagaaa tatctctttc atgccataat gtgacatttt cataggcagt gaccatgtgt
      481 ccgcgcacca ctctggctag accagccata ttttctgaac cgatcggatt ccttttgtgc
      541 ggcatagcag aagaaccttt ttgacctttc gcaaaaaatt cttctacttc acgtgtttcg
      601 gatttttgca atccgcgtat ctctgttgca aaacgttcaa tacttgtagc aataagcgcc
      661 atcgctgaga aatattcagc atgtagatcc cgagggagaa cttgagtaga gatttcttgt
      721 ggacggatcc ccaattgttt acatacatac tcttcaataa atggcgggat atttgcgaac
      781 gttcccactg ctcctgagat ttttcctgct tctactcctt ttgccgcatg ttcaaatcgt
      841 tcgatgttcc gtttcatttc tgaataccat gtggcaagtt tcaatccaaa agtagttggt
      901 tcagcatgga caccgtgtgt ccgacccatc atcactgtat atttgtgttc ttttgctttt
      961 tcgccaatga tttccgtaaa acgtttcaag tcttcccgta aaatatcatt tgcttgcttg
     1021 atgagatagc cataagcagt atctacgaca tccgtacttg ttagaccata atggacccat
     1081 tttctttctt ctcccaatgt ttcagaaacc gctcgtgtaa acgcaacgac atcatggcgt
     1141 gttaattttt cgatttccaa gatccgatcg atatcaaatg atgcatgctc acgaattttt
     1201 ttcacatctt cttgcgggat ttcaccaagt tctgcccaag cttcatctgc taaaatctcg
     1261 acttcgagcc acgcacggta tttgttctct tccgtccaga tattccccat ttcagggcgt
     1321 gtataacgtt ctaacatgtt ttttctcctc taatcccaaa tattcgtttg tttgatttct
     1381 tccagcgttt gctggatgtc ttctgtcaag atagtgatat gtcccatctt gcgacctttt
     1441 tttgcttgtt tttttccata gaaatggaat tgccagtctg gcttattctc aatcaagcga
     1501 tagctttcca gcagttgttc tcccaaaaca ttgaccatta ctgcattgct tatcaaacgt
     1561 actttttctc ctaatggcca accacaaata cctcgaatat gggcatcgaa ctgactcata
     1621 gaacaagctt caatagaata atgtccagaa ttgtgaggac gaggagctaa ttcattcaca
     1681 tatagcccgc ctgtttttgt taaaaacatt tctaccccta aaacacctga aaggctcact
     1741 gcttctgcaa tcacacgggc aatacgctca gcctcctcga tcacatcctg atcaatcgcg
     1801 gctggagcaa tcgtttcatg aagaatattg ttgtggtgga tgttttccac gaccggaaaa
     1861 gtcgtgaaat ctccttgacc atttcctgcc accatgatcg aaatctcttt ttcaaacggg
     1921 atccatgctt ctaaaacaca ggttccttct cttaaaaggt ccatggcagg agctagatcc
     1981 gcccgacttt tcaaaacata ctgccccttg ccatcatagc cgcctcgagt ggttttcaaa
     2041 acacaaggat agccgatccc gtcaatcgca tcttggatat ccgtcggact gacgatcgtt
     2101 gcataaggag caatcacgat gttattcgtt tccaaaaaag atttttctaa caggcggtct
     2161 tgcgtaatcg caagcaagtc tgtcccttga ggaataaatg acatgggtaa aatcgcattt
     2221 aacgcctcta cactgacgtt ttcgaattca taggtaatga catctgttct tctggcaagc
     2281 tctttcagcg caaaggtgtc atcatagaca ctttcaatgt gccaatctgc aacttgtgcg
     2341 gctggacaat cgctcacagg atccagcaca cctactttaa agcccatttc ttttgcacta
     2401 atcgccatca tacgacctaa ttgaccgcca ccgacgatcc cgatcgttga accaggcaac
     2461 aaaggtttaa ccaagttgat cactactttc tataacagtt ttagccaaca ttgaacgtct
     2521 ttcagccaat ttagcctcca attctaaatc atacatggaa agcatctgta tcgcaagtag
     2581 tccagcatta attgctcccg cttttccgat tgctgtagta gctacaggta ctcctccagg
     2641 catttggaca atcgaaagaa gcgaatccaa accatttaat gttcgtgatt ggaccggtac
     2701 accgataacc ggcaaagttg tttttgatgc gaccataccc ggcagatgcg cggctccccc
     2761 tgctcctgca ataatgattt tgattccttt ttttcgagcg ttttgtgcat attcaaacat
     2821 caaatctggg gttcgatgag cagaaacgac ctttttttca taagacacac cgaattcatc
     2881 caatatttga catgcttctt tcattgtttc ccaatctgaa gtactcccca taatcacaga
     2941 gatttttgct gtcattgttt ttcctcccag tttttctaca ttcatatttt aacaatcact
     3001 ttttttgatg tcaaagaaat ttcgaatgtt aacaaagaaa aacattttat tgttcgtgtt
     3061 tattaaagat aaaaaaaagg aatgtgacaa aagttaggtc acattccttt ttatggataa
     3121 acggtgttct cctgccaact cctcggtcac aaggaacaat attcaacaaa catgagaagc
     3181 ttttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttcct
     3241 tcaacggtgt tcaatcagct gaccctcggt attagctcaa acaaacggca aaatacgagg
     3301 aactattttt ccgtttgttt ctctaacaat caggtcaaaa cgctgatttc acaacctttc
     3361 ttttgctcta ttacttcttg aaggcttctt ccaatatttc ttcatctttt ttcaatccat
     3421 taaagatcaa attcaatatg atagccgtaa tgctactcat tacgatgcca tttccagtga
     3481 acatttgcaa tgtttgcggc atttgactga ataatgtcgg cgtaacattg aaacccagcc
     3541 caaatccaac agaaacagca acaatcaata gatttttatc attgctgaaa tcaacttcca
     3601 acagcatacg gattccttgg actgccacca tcccgaacat gactaacatt cctccaccaa
     3661 gtaccggctc agggatgatt tgtgctaacg caccgatttt agggaataat cctaaaacaa
     3721 tcaagaagaa tgcagagaaa tagatgggtt ttcttgtttt gattcccgaa agttgaacca
     3781 atccgacatt ttgagaaaag ccagtataag ggaatgtatt gaaaataccg cctaagataa
     3841 cggcaagtcc ttctgcacga tatccctttt tcaattcttc ttctccaaca tttttcccag
     3901 taatgtcacc aagtgcaaaa tatacacctg ttgattctac catgctgacc atggaaatga
     3961 tgatcattag taaaatcgac catacgtcga aagttggttt accaaaataa aagggttgtg
     4021 gaacatggaa caacggcgca tctccaatcg cgctcagatc aatcattccc atgaaagcag
     4081 ctaagatgct tcctccgact aaaccaatca ataccgcgat tgaacgaatg aacccttttg
     4141 ccagcatttg cacgcctaca attaggagaa tcgtcacaaa tgcaagtagc agatttgttt
     4201 tgtcaccaaa atcagctgct gttgcattac ctccacccat tttctcgatt gctacaggaa
     4261 tcaaagtcaa accaataacg gtgataaccg ttcctgttac tataggaggg aatagttttt
     4321 taattttaga aaagacacca gaaactaaca caacaaagat cccagaagcg ataatagatc
     4381 cgtagatagc tcctactcct tcattggttc cgatcaagat cagtggagct actgcttgga
     4441 tcgcacatcc taaaacgaca ggcaagccaa ttccgaaaaa tcggttaact gtcaattgca
     4501 ataatgtcgc taatccacac ataaagatat caattgaaat caaataagtc atctgttctt
     4561 gattaaagcc tagtcctgta ccgatcaaaa gaggcaccgc tactgctcct gcatacatcg
     4621 ctagcagatg ctgcagccct aaaaccgcag cttttccatt ttgtgtttct ttttccacga
     4681 ttatgcgtcc tcctcaagaa actcaacttg tccattcgtc aaggaagcaa tacgcgcaag
     4741 agaaacgact ctcaatccca tttcttcaag taactgacgt ccatcttgga aagacttttc
     4801 gatgacgatc ccgattcctt ctacttgcgc tccagcttgc tggcataatt cgatcaatcc
     4861 ttttgccgct tgtccgtttg ccaagaaatc gtcgataatc aatactttgt cttctgaaga
     4921 taaaaattta cgagaaatag aaatcgtact tgtcacttgt ttcgtaaaag aataaacaga
     4981 agctgtcaat aattcttcat ccatcgtcaa actttttgct tttctagcga agatcattgg
     5041 aacatctaat tctcttgcag caaataaagc tggtgcgatc ccagaagctt caatggtcac
     5101 aactttcgtg attccttgtt ctttaaatac ttctgcaaac cgttttccca tttgttccat
     5161 caatacaggg tctacttggt gagtgacgaa gctgtcgact tttaatacgc cctcgcctaa
     5221 tatacgtccg tctttttgaa tacgttcaac taattctttc actttatttg ctcctttatc
     5281 atttattaat accgaaataa ccgcttttgt gcaaaaacct actatgtttc tgttaaagaa
     5341 aaacaccctc cactttatca taagaaaaga agggtgaaat agccaatctt ttacttatag
     5401 tccagcattt acggtactgg ctagaaactg tcatccatat tatgacgata tataagtagg
     5461 tatgtaaaac taataatagt gtaaattcat tttcttgcaa ctgttttgca cgtgaaggca
     5521 gtttgccata tttgaataaa aaatgttgtt tcaaatattg aaaacagtct aacactactt
     5581 tctgaaactg acaacgtggt ttacttattt ttttgtaaaa tctaatgatt tttaatgcat
     5641 tattcctttt tgttcgggtt taaatagtta tttttatttt ctcctatcta aaaaagcagc
     5701 ccacataaaa agaaggcatg acaaaaagca tgtcatagcc cctttttccg aataaatagt
     5761 gttcaaaaga cagcctatcg gcaataaggc aagattttat aaactacggg aagtaatttc
     5821 cgtaaatcta ttcttattct tttaagctga caagctcgtc aatcactact gcttttcctg
     5881 aagcaataga atgatttgct gcgattcctg tcaaaatcga caaaatacca tctacgtgag
     5941 ttgctgcccg attccacgga tcttcttctt tttcttcacc aaatagatca tctaataaac
     6001 gcggatctcc tccgccatgt ccgcccgctt cttgttcgac gattacctca tatggctcac
     6061 cgaacatcgg atagacatgg atggactgaa gaacggctgc tccttcatcc tttttatctc
     6121 cacctgcatt gatgtaagac ttttcgatca ctttcatttc gatccgtcct ttgctgccat
     6181 tgaaagaaac attgaatcct tcccacggca tatacgcgtt tagcgaatac gtcaaaatcg
     6241 caccagtttc atatttgacc gttacgccta gtgtatcttc aatagagata tcatcggaaa
     6301 atacactgcg gtcacgaata tatccactgt ctttttctgc ttctaaatac atttctcgca
     6361 attcctcatt ggctgacagg tctatagcaa atggatcagc tttagctagt tcgcttccat
     6421 agcatcgact gtaaaatatt tctactccgc gtttctccgc attatgcatc ccataaaagt
     6481 ttaatccacc gaatgcaaat accttttggg gacggctgtt caaccaaaaa ttcactagat
     6541 caaaatgatg ggtagattta tgaacaagaa gcccgccgct attgtgctta tttcgatgcc
     6601 atcttctgaa ataatctgcc ccgtgttgcg tatttaacag ccattcgaag tggatagaaa
     6661 acacatcacc aatcacatca ctttctatca gttcctttat cttcgtgtga tacggtgcat
     6721 aacgataatt aaaagtgact cgcaggcttc ttcccgtctg tttttggacc gttattattt
     6781 cttgagcttt ttcctcatcg atagtcagag gtttttcgca aataacatca tatccttttt
     6841 tcattgcacg aatgatataa tcatgatgcg ttcgatctac tgacgtaata atgacttcag
     6901 ccggccgtac gtcatccagt agttgctcaa attgtgatgg catacatgtc tttacttttt
     6961 tctgtccata cttgttcaca agtatccgat ttgcataatc catccttgtt tgagatacat
     7021 cacaaaaacc gactagttca caagtttgat gatacgtaga ggcaattgcc tcataaaaaa
     7081 aacgcgctct cccaccaagt ccgacttgta catatttttt cttcatttaa agtcattctc
     7141 cttttctttt aagcaaaata ctgtgacaaa tggcagccct ggatacagta atgcagtgag
     7201 taaaaaatca tgctgtatac accagaacag tccatacaaa aggcataaat accacagaaa
     7261 ccccttgacg ttttttgaca acagccagat attccttttg atcaaataaa caagactatc
     7321 tgctttatct tctttgcaga tatcaattag cgtactaaaa aaactatggg atacaaaaag
     7381 caacagacac aatagaaaag gaatcagata ccaagctcca atctttccca ctaataaata
     7441 ccaatcaact gcaaaaataa tgacaaacag ctgaaacaac gtaatgatga aacacatccg
     7501 tttgattttt cctttcctcc aacatacctt tttttctgcc aagtaatgga cagatagata
     7561 ataagaagga tacatcatca aaaaataaat cagatagaca acacgagcag acagcactaa
     7621 tcctaaagtg aacaaacatc ctagaactaa agcgaaaaga gcattactga taagactggt
     7681 taaaaaaatc cgacagacat agtagttgag cttgtaaaga aacatctctt ctattggctt
     7741 tttcatattc agcccttcag accgcttgta ctgatcccct ggacaagata tttattgaag
     7801 aataagaaaa tcagaaaaac cggtagcagc gacaacgtag acatcgcaaa catactcgcg
     7861 taagtcgtac tagatccagc gtctgcaaat aatttgatgg caatcgatac cgtgtaagat
     7921 ctcggcttgt ttaaataaat caatggtccc atgaagtcgt cccatttcca ataaaactgg
     7981 atgatgatcg tcgtgatgat cgaaggtttc agcaaaggaa aaataatttt ggtaaagaca
     8041 ctatacttgc tgcatccgtc aataatcgct gattcatcta actctttggg gatgctgacc
     8101 atgaattgca tgatctggta aatgaaaaat gcttgtccaa aaaaatgtgg taaaatcagc
     8161 ggcacatacg tcccaaccaa attcagccga ttgtaaataa taaactgcgg aatcaaaatc
     8221 acttgtgcag gaatcatcat cgttactaac atgacgacga accagaatct ttttcctttg
     8281 aaatcgattc tcgataatgc gtaagcaata caagtcgagc tcagaactgt ccctatcgtc
     8341 gaaactgcag agatgacaaa cgaattccta aagaaagtaa aaaatgaaat tccagcaaac
     8401 cccgcccatc cttttgcgta attttcccat tgaaaagtct ctggaatcaa attcagcgaa
     8461 ccactcataa tctcttgatc cgttttaaaa gaacctgcga tcatccataa gaccggataa
     8521 atcatgacta gacctaatag caaaataaaa ccgtattgaa accatttcgt tactgtatta
     8581 ttcatttctt cctcctctca ttcacacaac gacccatttt tttgatgatt tgaaaatcag
     8641 aaaggtgaca gctcccataa tcaacagcat gatccacgaa atagcactag cataccccat
     8701 agaattaaaa gaaaaagctt ggttatacac ataaagagca taaaagttcg tggcatcatt
     8761 cggaccacct tgggtaatga taaatgcctg cgtaaaagtc ataaacgccg cgattgtctg
     8821 cataaccaag ttatacaaaa tgatcggcga aagaagaggt aacgtgatct tgtaaaacgt
     8881 ctgccatttg ttcgctccat cgatctctgc cgcttcataa tacgagcctg ggatctcttt
     8941 gatacctgca gcaaagatga tcattgccga accgaattgc caagcagaca tcaaaacaag
     9001 agggatcatg accagttttt gatcgctaaa ccaggaaagc gcaggtgcgc ctaatcgttc
     9061 caggaaaata ttcactaacc cgtttttaga aaacaactcc cgccaaacta gagcgataga
     9121 aatactgcca ccgatcaaac taggcacata atacaaagaa cgaaagaaag tcgttccttt
     9181 gatattcctc gttaaaagat aagcaatcaa tagaccaaag atcagtttca caggtacaga
     9241 tgcaaataca tagagaaaag tgactcggat ggagttcaaa aaacgggcat cactcagcat
     9301 ctttatataa ttatccaagc cgatccatga gactggattt cccaaacgat aatcggtgaa
     9361 cgaataataa agtgacaaga tcatcggtat cactgttatc aatagaaatc ctaggatgaa
     9421 tggagaagca aatacgtaac cgaccacatt gtcttgatcc aagaaccgtt ttagtttttc
     9481 ttttttcatg ccttcctcct gctgctatcc tagaatgttt gacgcaaact cataaagact
     9541 ctctgctgct tcttctgctg ttacttcttc ataaatgact ttttcaacta aaagattgta
     9601 ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc cgttgtccat
     9661 atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat ctgcacgagc
     9721 gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta aaatttcatt
     9781 ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt tatgggctga
     9841 gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt ctccatctgc
     9901 ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc ctgacaatat
     9961 ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc taaccgcctg
    10021 aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg cccctttgtc
    10081 taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt caaatcccaa
    10141 gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt gtggtatttg
    10201 aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc ctaattcttc
    10261 agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag ctggttcagg
    10321 gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat tcaccccgtt
    10381 agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt catctgcatt
    10441 agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct gattgatgta
    10501 ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg ctgctagtgt
    10561 acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg tcacatgggg
    10621 atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga cttgcgaacc
    10681 ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag ccgtcaagaa
    10741 gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc tctgcacctc
    10801 tacttccatt gttatttttc aatatactgt atttttatac agaacatatt tattaaacga
    10861 tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag gatgaacaac
    10921 tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta aaaacacttc
    10981 ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac aactgcccaa
    11041 tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct aaaaaacaga
    11101 aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg cacaagcatt
    11161 gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac aatttagctg
    11221 ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta tagaaagcgc
    11281 ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg ttttaactct
    11341 tcttatgtac attacaggta aaaaaacaaa aaaatttctc ttttttttga attctgaaca
    11401 aatagtactg gagtacaaaa gacgaggaag agccactatc tgctcttcct cgtcttttga
    11461 ttctcacctg ttattcatga ttctttttct cagtttcccg atatttttcc attttacgac
    11521 taaaaagctc tccgctactt ggtggcgtat atgtgatggc atttgctcct gcttgaatgg
    11581 tctcagcaat actttcttcc gttggaccac ctgtcgcaat gataggaagt tcaggatatt
    11641 ttttacgaaa atggcgtact gcttctgcag tttctttacc tgcactgata ttgataatcg
    11701 aaataccagc tgctaacttt tcatctattt ctgtatattt cgacgttacc gtactgataa
    11761 ctggaatatc aactactcga caaacttctt ttactgtctc taaaggcgta ggaccgttca
    11821 aaacgacccc taacgaacct tgcgcttctg cgaacatgct catgtaggaa gaacgagcac
    11881 cttgtgtcaa accaccacct acaccggaga aaactggaat gtcagctgcc tcaataatgc
    11941 ttttagtaat cgctggatga ggtgtaaaag gatacacagc aattactgca ttcgcatttg
    12001 tattacgtat gatcgcaata tccgtagtaa agatgatcga acggatcaat tttccaaata
    12061 tcttgatccc gcttgcttct aaaatgattt ccggtactct tacgatatct tttctcaatt
    12121 ccgtcatgat ttctggcgta aattgttctg ccatcagcca cttctccttt gctatctccg
    12181 gtcatactga ttcaattgat acgtctcgat gacctggatg attttttgcg cataaccggg
    12241 gtctgtcgca taacctgcat cttgcaatgc ttgtgcggct tcctgatagt tggtggccgt
    12301 aatcactttt tcatacagcg caggattcca atctactcct tggacgaaca gttgtgtatg
    12361 gtcatccatg gactgttccc atgaatcata cactttgaag tctccttgga tagttatcca
    12421 ttcttcgtta acaaattcct ttgtttccag gcttactttc ttctgatccc cataagcctt
    12481 gatcccgaaa agattattgt atttgctagc caaggtactt tttccccaat ttgattctaa
    12541 tatagcttgt cctaaaatga tactaggaag cactccataa ccatcttgta gttcttgggc
    12601 atgcggaaca agccgttcaa taaattgctg atgggattgc gcttcatttt cttgctgatt
    12661 atcagtaaaa ggagaagaca gattcttcaa ggaaaaaaca aaggctaaga gtatcactgc
    12721 tacaccggca accactgctg gccaacggat attcttgcgc ttttgctttt tccacttttt
    12781 tttcgccatg atcaattcct acttttcatt taattcattc agatattctt ctagcgtcaa
    12841 ttggtgtttg gtgatatact cagcgttttc tttaccgaca taacgtaagt gccaaggttc
    12901 atatgtgatc ttagttatat cttcgtgtcc ttttggataa cgaacgataa atccatactg
    12961 cgcagcatta tctgcgatcc atttagctcc aggctgatct ccgtaacttg catccaatac
    13021 agtagaagga taactgttgt accagttttg atcgacgaca tcgactgcca gacctgtgtg
    13081 gtgttcgctg tatcctggct ccgtaatcgt ttctttagta atctttgtcg cctcctcttc
    13141 tgatacacct tgacgactca tcacatcttg gacattttgt tcgaaaactt gttgttggga
    13201 tgatacagac cggtaagctg agaccatcac taatggaaat ccagctgctt cagcagcctt
    13261 ggcaaatgct tcatattctg gagcaatgcg tttgtcgatc aagtatccgt tacttaatga
    13321 tgccaattga gtagactcat caatttcttt ttctaatttg tgatctggac caaccaaaac
    13381 tagcttccaa tcgtctgttt taacatctgg caattgtttg gacgtacgat cttttgtttc
    13441 tttgtccttt accttgctgc tggttgtttg actgttcttt tctttactag cagttgtttt
    13501 ggtgcttgtt cgttcgactg cttgattgcc ggcttgaaca tgcgaatcat catcgctaaa
    13561 aataacatag cccattgctg cgatcataat cacaactgct ccaaatccta aaaatttatt
    13621 cacttgaaaa atcctttcgt tgttcttcac gttccccatt cgcctcatga acgtaagttt
    13681 cttccagatt ttcattcacc caatcgagaa gttcgatttt ttctccttcg atctgttttt
    13741 gaacatctgc tggcagacga aaattcaaaa tatcatcata gccccattca tcatattgaa
    13801 tacttacttt caagtcaagg tatctaccag cctgctgatt atttttcaaa gggatcagct
    13861 cgacttttgc tgctttgctg ctaagccatt tttgcgcgat gcgtgttttg atttgcttgt
    13921 actctgcaac ggcaacgttt cgtttttcgg gaaaaataat cgtttgacgc aaaatctttt
    13981 gcgttagcat ccattcataa tcagtgaaaa gattttttac tttctgcatc gcttcagatg
    14041 gcagattttt ctcgccatct ttccattttt gccattgttc tggactgatc agcaagtatt
    14101 catctaaaaa atttttttca gtttcaaact gttctaataa tacactcata ataagatcaa
    14161 tatatacttg atgcacgttt atcccctcct tttaactatt ctacttaagt tttgatcaaa
    14221 aagcacctct taaatcgctg ctttcatttt ttttaatgtt tcaagtcctc tcctctttat
    14281 tcttcgtgat ttttgttata ataatagaca gacaagaaac tcaatccaac tatgaaggag
    14341 cgttttcaga tgaaaaaata tcaatgggga atcgttggtc ttggtaccat cgcacatgag
    14401 tttgccgaaa gcttcaatca agagacgagc gaactaacag cagttgcttc cagaacttca
    14461 gaaaaagcgg aaaacttcgc tcaccgttac aatattccaa aagcttacgg aagctatcaa
    14521 gaaatgttag acgatgctga aatcgacatc gtgtacattg ctgtaccaaa tagacagcat
    14581 atcgaccata tcttggcagc attggaagca ggcaaacacg ttctatgtga aaaagccatc
    14641 acgatgaata aaaaagaact ggctgacgct atgcgcttag cagaagaaaa gaatttgatc
    14701 ttagcagaag cgatgacaat cttcaacatg ccactttatc agcagttacg ctcaataatg
    14761 gatacaggaa aactgggggc tttaaaaatg atccaagcac ctttcggcag ttacaaagaa
    14821 ccagacccta aaaaccggtt cttcaatcca gaattagctg gaggagctct gttggatatc
    14881 ggtacgtatg ctgtatcttt cgctcgtttc tttttgagct ctcaacctga agtagttgcc
    14941 tctacaatgg ttccttttga aacaggcgtg gacgaacaat ccgtcactat cttgcgtaac
    15001 aaagaaaatg aacttgctgc tgtcagcttg acttttcagg caaaaatgcc aaaagtcggt
    15061 atcgttgcct ttgagaacgg ttatgtgacg attgctgatt acccgcgtgc tgatcgtgcg
    15121 gagatcttat ttacagatgg tacaaaggaa ttcatcgaaa gcgggaatac atctgaagca
    15181 atgaattacg aaataaagaa tatggtacac atgattgaag gtgacttgcc caatcgctct
    15241 ctcttcttga caaaagatgt catcgaaatc ttggatcaaa tgcaagttct atggaaaaat
    15301 atgtaatttt aaaaggttgt gaaaaagctg ttctgcatca agtaataaga acagccaaac
    15361 aaaaatagct cctcctattt ttcgtttggc tggacttatt accgcagagg aaagcttttg
    15421 aacaccgttt aagaaagagg ttgtgagcct accgttcagc tccgagtcac aaggagcaat
    15481 ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttgacag
    15541 gagaacaccg tttaaagaag aggttgtgaa atcagctttt tgacctgaat attagctgaa
    15601 ttcacaacct ctatttttgg attttttgtt gggagaattc tgccttgatt tcaataaaac
    15661 ttttaaaaga ctgcaaaaac tggaacaatt ctgctcgatc ctcgaattca gggcgactag
    15721 tcggcagagg catttgccga tacaatacat aaacttcttc aatacgtgtt aaaatttgtc
    15781 tgccatcatt tgactctgaa aatgtttctg cagtataaat caataaacca tagatatgtt
    15841 ttccataata agctggttgc tggattctct ctagattttc cagcatgtct ttaatcacat
    15901 tggcctgtgc tcttctcata gagaagtaag tttcgtagta tagcggctgt ctgagccagt
    15961 agttttcttg atgttctctg gcatcttctt ggctttcgcg aatgaatgtc agtaaatgtt
    16021 cgcatttgat ctgcacatct tctttatttt ctgataaaag aaattctgcc atttgtctca
    16081 aaatatttcg aaattctttt tcgatctgta tttggttttc cttcagctgc ttcgtacgat
    16141 ctggcatgta aacatttgca agcaaagcaa atactaatcc aatcgccatt aatcccgctt
    16201 cattgccgat cagctgccaa gacatgtttt cctccactaa ataatgagtg accaaaaccg
    16261 aatttactac tatcccttcg gttaatttga agcgagaggc aagcgggatg aacaggaata
    16321 aaaaaagact gaagccaaaa atagtatacc caacagcaaa aaaactgaac agtgctgttg
    16381 ccgtagctaa tataaaggct gcgattcgat tcatgcccgt acgcaatgta gagcgcttgg
    16441 tatttcctac acttaataat gcaatgatcc ctgcagctgg ccaatacaac agcgataagc
    16501 tacccgctgt gaccatcgaa aagaagacac tgatcactgt tttgactgtt cgcattccta
    16561 tacgcattta cttccccctt ttttatttta attatacttg aataatgaaa aactagctat
    16621 cagacaaaag tttaggtatg atagtaaaaa gaccaaaaag gagaatctct atgcaatttg
    16681 gccgttttcg actaggtatg cgtacgatca aaagtgcctt agctgttttt ttatgtatct
    16741 tgttttttca tttgactcaa cgaggaatcc cgatgattgc tgcattatct gctgtttttt
    16801 ctttgcgtca ggacttgact acaaccgttt cttttggaaa gtctaggatc atcggcaata
    16861 cattaggtgg tacattagct atcatttatt tttttgtcaa aagctatttt cacaatgatt
    16921 ttttggtaga attgatcttg cttcctattc tagttattat tgtgatcgtc gtgtcagatg
    16981 gtattaacaa taattccggt atcatttccg caatagctac gttgttgctg attgcgttga
    17041 gcatccctca aggcgaatcc gctttgtatg ctatccaacg tgtactagat acttttatag
    17101 ggacattcat tgcaatcggt atcaatttct ttcttcgtcc tcctgaaaca gaaaagaagg
    17161 aagaactatc agaagatttg gtcgaactcc aaaagaaaga acgattgcta agaacaaatt
    17221 tacaagagat agaagctaag atccaaaaac aaaagaaaaa tagcgaggac taattttctt
    17281 ttgcaataaa ataaaaaaat ggagagaaga actttaaaaa gtcttcctcc atttttgatt
    17341 gaacggtgaa gtgtactact ttattcttcc ttcatcgagt tttgttctgc ctcttttggc
    17401 gcttcataca tatttacttg gctagtagtc ccacgtgtcg acaaattctc tacgatttcc
    17461 ttcaagtcaa gtcctgttgt cgcttttagt gtttcttgag tagaagaaag caaatttgtg
    17521 gcataatttg tcactcggtt cgctccactg gtttctcctg aactgcttcc tgtatcgaca
    17581 acagagattt tttcgatatt tcctagcggc tgtgctgctt cgcgcatcaa ttgcggtagc
    17641 atatcgataa ccatactcaa gacagctgct tcgccatatt ccttgaaggc attggcgatc
    17701 ttttgttttg cttcggcttc tgcttttcct tttgctagtg ctgcttcggc ttctgcttgt
    17761 ccagctaaac gtgtcttatt ggcttccgct tctgccagtg cttccacacg gaatcgttct
    17821 gcttcggctt cagtcacttc tttgactttt tgagcttgtg cttcttgttc tttagcgtac
    17881 cgatcggcat ctgctttttt cttcacttcc gaatcatatt gcttttcacg acgtgtgatt
    17941 tctttttctt ccagttcgat ttgtttttga cgttctacga ttttaatctg catttcctgt
    18001 tcgattactt gttgttgtgc tcgggcactt tctaaattgt aggcttggtc tgcttttgct
    18061 ttagcaacat cttgttcttg tttgtaggtt gctaatttca gttctttttc ttttaatgat
    18121 tctgcgatct ctgtttgtcg ctgtaattca gctgcttgtg attctttttc tgcttgagct
    18181 tttttgatac gcgtttcttt tagagcttcc gcttctgcta tatctgcatc tcttttgact
    18241 tgggcgattc ttggtttccc taaagagtca agataaccgt ttttgtctct gacctctttg
    18301 atagtgaaag aaacgatgat caaccccatt tttgcaaggt ctacagaagc gacttcttgt
    18361 acgctttgac tgaacttatc gcgattttga tagatttctt ctacagtcat agaaccaaga
    18421 atcgagcgca agtgtccttc taatacttct cttgcttcat tttccagttc ttctcttgtc
    18481 ttgcctaaaa actgttctgc tgctgtagca atctcttcta cagatgaacc gattttgatg
    18541 atcgaagtac catcacacat caccggtacg ccttgttccg tatacacttc aggggtggag
    18601 acgtccaatt tactcgataa tagactgatc cgattcgaac gttgaaaaac tggcaagacg
    18661 aatgcgccac ctccacgcac aattttgatt ttgttattgc tttcatcaat gtgcacattt
    18721 ttgcttccta aataacttcc actgatgatc aacgcttcat ccggttttgc tgtctggtac
    18781 ttcgctacaa agacgatcag caacatcagt aaaatcaaag ctgcgattgc gatcgcgata
    18841 aaaacaactg gtatttccat ttaacacacg ctcctttaag cttttatcat gctttcataa
    18901 ggcatgacat aacagattct ttccttgatc tcaatgatca atactttcgt tccccgctca
    18961 gctgtctgtt cttgggggct gtaaaaacaa gctggacgac taatggaacc ggtaacactt
    19021 ttcaactgaa tttctcccat acctcgtaca gggatcggtg tgatcacaac agctacttgt
    19081 ccttccagag ttttttcaga agacgacatc gttgattcgg catgtttcat cggcatcaat
    19141 atatagaaat taagcaaaaa taccaacact atcgatatgc ctccactagc aataaataaa
    19201 aataaagatg attgatctgt taaacgctca cctagaaaac caaataagct tgtgaaagtt
    19261 aaccaaggga taataagcat cggatcaagc gggccatcaa aatcgaatac atctccaaaa
    19321 ataatcaata ggactgccaa tcctgcacaa atcagcaatg tataccaata aatcgtctca
    19381 agtgcgattc cccctatcat atacacgcct ccttccattt ctacgtctat cataccaatg
    19441 tttgaaaatt ctgtatagct tcttttaccg actcagtggt gttttttata ggaaggttgt
    19501 gtttatcgtt gacaatttgt taaatttttg ttttcatgtt aaaattttca caataaattc
    19561 tttcgaggta gcctatgttt ccttttttta agaaaaaacc attaaagctc cagaacacga
    19621 tcatccttgt cgtttgtttc tccacattcg tttctttgat tgtttctgcc atattgatca
    19681 ggaattttat tctttcgatg aaccgatcta gtgtgtcaga taaaatctct agcattgcta
    19741 aaatcgttgc caatgataaa ctcatccaaa aaaagctgca gactggtgac ccgacacatg
    19801 cgattcaagg aataacaaat gaaatcatga ttgcatcgaa tttagatttt atcgttgtca
    19861 tggaccctga aggcattcga tattctcacg cagacccaag ttacatcggt cttcctttct
    19921 caagtcatga agatgtgaaa aaagtgattg gaggacaaga atttttctct gaaaaagaag
    19981 gaatgttagg gttcggcttc cgctatttcg tccctattta ttatgatcaa gagattattg
    20041 gtgcagtttg tgttggctta acaaacaaca cgattgacaa tcaagtcaaa gatggacaaa
    20101 aaatgattgt tttttcattg attattggac ttagcgtagg catcataggc gccgtttttc
    20161 ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc acaagaaata gcagaacagt
    20221 taagtgaaaa aacgattatc gaaaatgaag tagatgaagg aatcttagcc atctcttctg
    20281 ataaaactat cctcttgatc aatacacctg caaaaaacat tttctaccaa acagagcagc
    20341 tattggattt cgaagtagga aaaaaatcag tgataatcta tatgacgttt tgtttaaaga
    20401 gacgattgag catcaaacga ccgttcggac tcaatccatg tatttaaatg ggatagaaac
    20461 tatttctagc gttacacctc tttttagcaa tgaccgtttt ttaggagcag tcgcaacttt
    20521 acgaaatcga tcagaagtaa tgaaattaat ggaggagctt ggggggacga aacaatatat
    20581 aaactccatg agagctcaaa cccatgaatt catgaataaa cttcatgtca tttctggttt
    20641 gattgatttg aagaagtatg aagaagtcac ccattttatc cagcagctaa atcaaaatta
    20701 taaaaaagag attggccaag tctcaagtct gatcaaagta cctgcaattg ccggtttttt
    20761 attaggcaaa ataaacgaag ccaaagaaca atctgttgaa atgctgttag aaagcgattc
    20821 ttttatccct aaaatggaga tggatgactc catccattca cttttgcaag tgttagggaa
    20881 cttacttgat aatgcaaaag aagccgtgct acaaaaacat gaaagaaacg gacaggtccg
    20941 gattcattta aactatgaca cagaagctga agtattcatc gtgaccgtct gcgatgacgg
    21001 tgtcgggata tcagaagaaa tctcagaaaa aatatttacg ctaggttttt cgacgaaggg
    21061 aatcaatcgt gggtttggat tgaatctcat ccattcaatc gtcaaacgcc acggtggatt
    21121 tttagaattt tccaaacaaa caaatggcgg tacaatcgtc tatctagaat taccaaaaaa
    21181 ggaagatcaa aatgatgaca aatgtcctga taatcgaaga tgatccaatg gttgcagaat
    21241 tgaatcgtac ttttgtggaa agcatcccta atatgaacgt aatcgaaagt ttcagaaatg
    21301 tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga tctgctgctg cttgatgtat
    21361 acatgcctgg cgaaagtggt atccattttt tatcttggct tagacagcaa ggaaatgata
    21421 tcccagtgat tttgatcact gctgcagatg atatcgaaac cataaagaat gtgatccgct
    21481 acggtgcctt agattatttg atcaaaccgt tcactttgca acgttttcag ctggcgatcg
    21541 aaaagtatct tgctttctct cagacaatcc aaacgactga aaaagccgat cagaaattga
    21601 tcgatcaagt attcaaccac accactccca ttcacgaaat accatcattc atcgatttgc
    21661 ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc atttattcta aaagaagata
    21721 ctattttttc aacaaaagaa ctggctcaaa aaagctcact gtcacgtata tccgtcaaga
    21781 aatatttgaa ttttttagta gaacaagata tattgaacga aagaatcaaa tatttggaaa
    21841 tcggccggcc gctaacctgt tatgaattga caaagaatgg gcggaaaaag ctcacagatt
    21901 actgttgagt catcgaatta tcccatacaa aatagtccaa gtatacagaa gtaacaactg
    21961 tttacttgga ctatttttaa acattttatt gacgaatatc catgttttct tatcttaatt
    22021 gacgggcttt aggaagagat acctcatttt tcgtttttcc agtttctatt atttcacgaa
    22081 agttctcgaa acttgtcgaa atcatatttg ataatgcctc atctgtattt gaacctacat
    22141 gcggggttac aagaactcta gggtacattt ctactaattg ctggatagca gggtctggaa
    22201 tcgtctccca tggcttgaag gatcggaaaa aaacctctgt ttcatttgca aaaacatcgg
    22261 ttgcaaaacc agctaaatga ttgcttttca aagctcttaa aatagcttca ttatcttgaa
    22321 gctctccacg ggctgtatta atcaagatgc tacctttttt catttgagct aaaaatcctt
    22381 cattgatcat ttgatcgttt tcacctggga tatgaggtac atgaatactg acaatatcgc
    22441 ttttggctaa taactcttca agcgagtgga acgtcaatac ttttttagca gcttctgtct
    22501 gatgaatatc gtagccgatc actttggctc ctagtccctt aaacaaaaca gcttccgtca
    22561 accctatcct gcctgttcct atgatcccta ctgtgcaatt tctgatttca cgactaaaca
    22621 tttgctcatc cactcgaaaa tctttgaaag atgttcggat cgtcgtatac gcagtatttc
    22681 tcaaaagcat catggcaaaa gtcaaagata actctgctat cgcatttggc gaatacgatg
    22741 gtacacgggc tactgtcata ccaaaatcag ctgccgcttg tagatcgata tgattgactc
    22801 ccacagtccg tgtaaaaaca taacgaatcc catattcctg catttttgca agattggtct
    22861 catctgctat acaatttccc ctcaacaata cagcatcatg gtcctttgct gacacgatat
    22921 tgtcatgtgt caacagttct tcataaagac tgagatcata atggtaaata ttcaattttt
    22981 caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc gattctcata ctttcttcct
    23041 ccatttctta tgaaaataaa cctatgccat gaatcatttg taatacaacg atccatacag
    23101 gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc attggaggct aaaagtgcct
    23161 ccctatcaaa actgatagca taggctgctg ccactgttgc agctggtgta gccatcatga
    23221 taacgattgt tgccagtgct tcaaaattga ctggtagaat attcgttgcc gtcaaaactg
    23281 ctagaatcac gatattcaat gcaggtacaa ataccacttt gactagactg taataccatg
    23341 aagttttttc agatgcagct tttttgaagc ttacttcacc taatgttgcg ccgattgcta
    23401 gccacgccaa tggtgaactt aaaccagcta aataatccat cggctgaaat agccacggtg
    23461 cagtctggtc aattcgcaaa aacgctactt ggcttatctc tccttctgca cctgtaactg
    23521 acacttgtgg cagataccct tggaacagcc aaatgaaaag gccggcaaaa gtagcgataa
    23581 tgattggatt gacgaacatc gctttgatat ttttagcttc catcttcaag ccactcattt
    23641 ttatgtatcc ataagaatac aaaaagattc ggtagccaat attgaaaatt gaggaataca
    23701 tcacgccgac aggaccataa atcgcactga cgataggcgt tccgaaaaag gtcgtagaac
    23761 caaagatcgt caggacacgc aacgttgttt gcttatcccc tttatagctt aagaaaaaag
    23821 gttttgaaac tagaataaga ataatataaa taactaatcc ccaaatcaag acattgattc
    23881 cttgcatcag taattctgta ttgatatctt gcatgaaagc tcgaaatgcc agtgctggca
    23941 aagccactgt taagacgact ttcgatagaa tttttccaac agattcagaa aaaatcccct
    24001 tttttctaca aacaaaccct aataatataa taaaaactgt tgaagtgatt gcactgacaa
    24061 tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa gttcatcttt ctcctcctaa
    24121 ttatgttcat ttattcacaa taaaaaaagc ttttattttg ctaatacgta ttaacatttt
    24181 taattttatc tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac
    24241 ttatgtaaag tgatt
//
LOCUS       DAZBWR010000031        24231 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000031 DAZBWR010000000
VERSION     DAZBWR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.031"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..969
                     /locus_tag="ACHGT5_001132"
     CDS             112..969
                     /locus_tag="ACHGT5_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN7217132.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1084..2004
                     /gene="pstC"
                     /locus_tag="ACHGT5_001133"
     CDS             1084..2004
                     /gene="pstC"
                     /locus_tag="ACHGT5_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN7217133.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2004..2888
                     /gene="pstA"
                     /locus_tag="ACHGT5_001134"
     CDS             2004..2888
                     /gene="pstA"
                     /locus_tag="ACHGT5_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN7217134.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2903..3709
                     /gene="pstB"
                     /locus_tag="ACHGT5_001135"
     CDS             2903..3709
                     /gene="pstB"
                     /locus_tag="ACHGT5_001135"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN7217135.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3725..4483
                     /gene="pstB"
                     /locus_tag="ACHGT5_001136"
     CDS             3725..4483
                     /gene="pstB"
                     /locus_tag="ACHGT5_001136"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN7217136.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4496..5173
                     /gene="phoU"
                     /locus_tag="ACHGT5_001137"
     CDS             4496..5173
                     /gene="phoU"
                     /locus_tag="ACHGT5_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN7217137.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5411..5710
                     /locus_tag="ACHGT5_001138"
     CDS             5411..5710
                     /locus_tag="ACHGT5_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217138.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5751..6443
                     /locus_tag="ACHGT5_001139"
     CDS             5751..6443
                     /locus_tag="ACHGT5_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN7217139.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6440..7207
                     /locus_tag="ACHGT5_001140"
     CDS             6440..7207
                     /locus_tag="ACHGT5_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN7217140.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7307..8875
                     /gene="liaX"
                     /locus_tag="ACHGT5_001141"
     CDS             7307..8875
                     /gene="liaX"
                     /locus_tag="ACHGT5_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN7217141.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9172..9492
                     /locus_tag="ACHGT5_001142"
     CDS             9172..9492
                     /locus_tag="ACHGT5_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN7217142.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9492..9842
                     /locus_tag="ACHGT5_001143"
     CDS             9492..9842
                     /locus_tag="ACHGT5_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN7217143.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10009..10947
                     /gene="hprK"
                     /locus_tag="ACHGT5_001144"
     CDS             10009..10947
                     /gene="hprK"
                     /locus_tag="ACHGT5_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN7217144.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10962..11792
                     /gene="lgt"
                     /locus_tag="ACHGT5_001145"
     CDS             10962..11792
                     /gene="lgt"
                     /locus_tag="ACHGT5_001145"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN7217145.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11836..12861
                     /locus_tag="ACHGT5_001146"
     CDS             11836..12861
                     /locus_tag="ACHGT5_001146"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN7217146.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12876..13814
                     /gene="galU"
                     /locus_tag="ACHGT5_001147"
     CDS             12876..13814
                     /gene="galU"
                     /locus_tag="ACHGT5_001147"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN7217147.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13939..14379
                     /locus_tag="ACHGT5_001148"
     CDS             13939..14379
                     /locus_tag="ACHGT5_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN7217148.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14382..14984
                     /locus_tag="ACHGT5_001149"
     CDS             14382..14984
                     /locus_tag="ACHGT5_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN7217149.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15052..16158)
                     /locus_tag="ACHGT5_001150"
     CDS             complement(15052..16158)
                     /locus_tag="ACHGT5_001150"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN7217150.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16368..17369
                     /gene="ccpA"
                     /locus_tag="ACHGT5_001151"
     CDS             16368..17369
                     /gene="ccpA"
                     /locus_tag="ACHGT5_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN7217151.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17705..20050)
                     /locus_tag="ACHGT5_001152"
     CDS             complement(17705..20050)
                     /locus_tag="ACHGT5_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN7217152.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20329..21231
                     /locus_tag="ACHGT5_001153"
     CDS             20329..21231
                     /locus_tag="ACHGT5_001153"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN7217153.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21444..23219
                     /locus_tag="ACHGT5_001154"
     CDS             21444..23219
                     /locus_tag="ACHGT5_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN7217154.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23476..>24231
                     /locus_tag="ACHGT5_001155"
     CDS             23476..>24231
                     /locus_tag="ACHGT5_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7217155.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7737 a         4332 c         5084 g         7078 t
ORIGIN      
        1 ttttttttac aattatgaga atttattttg ttatttaatg attttgattc gttttcatca
       61 agtaatgaaa atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa
      121 cttaaattat tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg
      181 attgatcgag gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa
      241 acagttgctg aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc
      301 ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat
      361 ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca
      421 gttgttggga tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa
      481 aacctgaaaa aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat
      541 cagccagtag ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa
      601 tgggtattgg acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg
      661 cgccagatcg ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc
      721 gatgatgtag atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg
      781 aacaaatgga cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg
      841 acaaaagaat ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa
      901 ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt
      961 aaagagtaat ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac
     1021 caaattgatg ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca
     1081 tctgtggaag atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag
     1141 cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt
     1201 ttttattttg tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt
     1261 gattttctgt ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg
     1321 gtaggggcat tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt
     1381 gccacgccat ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga
     1441 acgaagatct tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt
     1501 tttatcggtt tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt
     1561 ggtatcttag ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg
     1621 actgttgatg cattaaaggc agtgccacgg cactatcggg aagcttcatt agcgcttggt
     1681 gccacaagat ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta
     1741 acagcagtcg tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc
     1801 atcggtaatg cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc
     1861 atcttaacaa tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg
     1921 tcattagcgc tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt
     1981 gggaagaaag gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg
     2041 ttttatatat agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct
     2101 tagttcgggg aataccgcat atttcatggt ccttcttgac cgaaccttcc aaagcctacc
     2161 aagaaggcgg tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga
     2221 tgatcatcag ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa
     2281 aaaactggct aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg
     2341 tcgttgtcgg tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga
     2401 ttatttctgg tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg
     2461 aggagtcttt acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt
     2521 cacgctggga aacagtgata aaagtggtgg ttccagaagc gacaccaggg attttgactg
     2581 gtgtgatcct aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg
     2641 gacaaagtgc acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga
     2701 tcagcatttt ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag
     2761 gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg
     2821 ttttactgtt caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt
     2881 cagcctaagg agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac
     2941 catgccaaaa gagaaagaag tagcactata tacggatgat ctacatgtct ggtatggcga
     3001 caatgaagca atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat
     3061 tggtccttcc ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg
     3121 gatcgctaat acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc
     3181 tcaagtggat gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc
     3241 gttcagcaag tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa
     3301 caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca
     3361 agtaaaagac agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt
     3421 atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag
     3481 tgctttggac cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga
     3541 ctatacgatc atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac
     3601 tgcctttttc tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag
     3661 accaaaaatt caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct
     3721 caacatgaca aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga
     3781 agcactaaaa gggatcaatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc
     3841 ttctggttgc gggaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc
     3901 gaatgtaacg atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac
     3961 agatgttgtt gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc
     4021 tttttctatc tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa
     4081 agtccttgat caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa
     4141 agacaaattg cataaaagtg ctttatcttt atccggtggg cagcagcagc gtgtatgtat
     4201 cgcacgtgtt ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct
     4261 tgatcctgtt tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac
     4321 gatgatcatc gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt
     4381 ctttttagat ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga
     4441 aaaacaagag acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct
     4501 acgtacacaa tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat
     4561 gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt
     4621 agcccaagaa gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa
     4681 gaaaagtttt gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat
     4741 tacggtcatg aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc
     4801 taaatccacg attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc
     4861 cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac
     4921 agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa
     4981 tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg
     5041 aacagactac ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat
     5101 ctgtgaatgg atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa
     5161 tgagattttc taaactgaag ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa
     5221 tcagcggttg tgaaaaagct gttctgcatc aagtaataag aacagtcaaa caaaaaatag
     5281 ctcctcatat ttttcgtttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt
     5341 ttattcggga aagggtttgt gacaaaacat gtcacaaacc cttttcatca tttcatagaa
     5401 aggataaaga atgcaaaaaa aaattagcat catcactata ttttttattt gtgtgcttac
     5461 ttcatgggtt attcagcatc aagctgcttt atatgaagta cctgtcatcg agatcgaaaa
     5521 agtacgaata aaagaggatc atcaattgat tacaggaaga ctgatgaaca cggatcaaaa
     5581 aggagaatcg atttatttat cggttcctta tcaaaaaaat caaatagaaa cagcaaatat
     5641 aaaagcgaga caaatgtatc tagtccaaat gcatggagat gaatggcagc tagtagcaaa
     5701 aaaaaaatga tggctggctt ttcttcttca caagtatctc cattggcgcc atgttgctta
     5761 ttggcggaaa acagggtcta tttgctttag gaagtctcat catcaatatc gttttacttt
     5821 tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat atatctatcc
     5881 tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg aatagaagca
     5941 gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt atttgtcttt
     6001 tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat tatctgacac
     6061 gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga ggaacactcg
     6121 ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa ccagaaataa
     6181 cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga
     6241 ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt ttatatttag
     6301 gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt atcattggaa tttttacgcg
     6361 tcattgctgg cagcttagga atagtattat cgatccctat ctcggtcctc ttttttctga
     6421 ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt tagcaatcag
     6481 cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat tcatcaattt
     6541 agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac tggcattgtc
     6601 ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg gttataatct
     6661 aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc tactattgct
     6721 tcctatcact gattttttag cgatccaagg ttttacatcg gtagaactag aagaattatc
     6781 tggcttagat aagactttag cgattgattt tagattactt acgagatcgt tgcttctgat
     6841 tagtcttagt ggtgctgttt tagatgctag tgtagcaatt tctagtggga cattcgaagt
     6901 atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt tcgctattgc
     6961 taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg gaagttcttt
     7021 agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga tcaatgaaaa
     7081 atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtctgcac tacttgttct
     7141 tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac aactcaagat
     7201 ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga
     7261 tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga
     7321 acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt
     7381 agaaagcatg gcaactgaaa aagacgaaaa gcaaataaaa aaagaagcag acaaggtaac
     7441 tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gataaacttg aaaacggcga
     7501 agaagaaatt tctgagccag ttgatccaaa ggaaagagaa aggcaagatc aagaaaactt
     7561 agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagatga
     7621 agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg aagcattgat
     7681 gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca
     7741 gacgttagaa gcagaaatca aagaattaga agctagtggc gatacactgt tagaagaaca
     7801 attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg agaaaaaaga
     7861 aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa cagatacact
     7921 gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa
     7981 aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag atgtcagctt
     8041 acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg aagctcctgc
     8101 agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag
     8161 caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg ctgaaccatt
     8221 tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct ccttccaaat
     8281 tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag tatacgacca
     8341 cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag ctaaagatat
     8401 ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga
     8461 agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat
     8521 cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaaccttt ctgtgtcttt
     8581 ggtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag
     8641 ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg aaggacatgc
     8701 taaaacaagt cttggaagta tcaacagccg tctgtccaat tacgaagtag ttcgtgagaa
     8761 aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg aaatcacgca
     8821 agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg
     8881 ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat agcttcccct
     8941 attttacaaa attgtgcctt attgccgata ggctatctct tgaacaccgt ttaccaagag
     9001 attatgaacc tgccaattat cgccgagtca caaggaataa tttcagaaaa agctcgtcac
     9061 gttttatgaa tatggtaatt tgtgaccgag aattttgcag aaagacaccg attaagaaga
     9121 aggttgtcac aacgattttc ttcatttaaa atagtagaac agaggtgaga catgatgaaa
     9181 aaaaagttga caaaatccaa taaaaatgtt gttttgacag ggactttagc aggaatagga
     9241 gaatacatcg ggattgatcc gactgttatc cgtgtgcttt atgtattcct gagcttagtt
     9301 gcttttggaa gtccaatcat tttatacatt ttattagctt tgattatccc atctgcgcgt
     9361 tctgcacgaa caaactatgg acataacaat gcatattatg aaaaaaacaa ttatcgtgaa
     9421 caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg
     9481 agtgactttt aatgtcgtac tttcaacgta ttttagttaa tacgttaaca ttcatatcat
     9541 tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc gttcttgcca
     9601 gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat tttgacgata ctgtccctgc
     9661 cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg attcttaaaa
     9721 tgacctcctt ttttgtggga gcagaacatt ttagtttttc ttcattcggt gcagcgttgt
     9781 tagtagctat cttgatgtcg cttgtcaatg taattgtcag tgaacataac agtagaaaat
     9841 aaaagaaagc tgtgccgaac tatcggcgca gtttttttat ttagaatcag gaatgcgttt
     9901 tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga
     9961 caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt
    10021 gtaaaagtta gccaattagt gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa
    10081 agcttagacc gtgtcatcgt aactggggac atctcacgcc caggactaga gttgactggt
    10141 tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt
    10201 gcggaacgga tgatcccaga agagcgtttg atcatcatga gaagattatg tagtgaagat
    10261 atgcccgcat ttattatttc aagaggatta ccggctccag aagaaatgat ccaggcagca
    10321 aatgaaaatg ggatagctgt actgcgttct cccatctcaa cttctcgttt attaggagaa
    10381 ttatccagtt atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg
    10441 gatgtttacg gattaggtgt actgattcaa ggtgacagtg ggataggaaa aagtgaaaca
    10501 gcgttggagt tgatcaaacg cggtcatcga ttgatcgcag acgatcgagt agatgtttat
    10561 cagcaagatg aattaacggt gatcggagaa ccgcctaaaa tcctggaaca tctaattgaa
    10621 atccgaggaa tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc
    10681 tctatgcaag tccagttggc tgtttatctg gaagcatggg caaaagataa gaaatacgac
    10741 cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc acaaataaaa
    10801 atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc gatgaatttc
    10861 cgtgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg
    10921 attgaagaaa actcaggcaa cgactagcaa ggagtgacaa tatgttagca caaatcaatc
    10981 gagttgcttt tgagcttgta gggattccta tctactggta tgcattgatc atcgtatcag
    11041 gtatcattat cgccatgtgg cttagcagca gagaggctgt tcgggtagga ttaaaagcag
    11101 atgatgtgac agattttatg ttgtggggat tgcctatctc cattattggt gctcgcttat
    11161 attatgtttt atttgatctt ccccagtata tagctgatcc aatccaaata ttcaatatcc
    11221 gttcaggagg cttagctatt tatggcggac tgattgcagg cgcgatcacg ctgtattttt
    11281 ttactaaata tcattttatt tctttgtgga cttttttaga tatcgctgca ccaagtgtat
    11341 tattagcaca ggcaatcggt cgttggggaa atttcatgaa ccacgaagca tatggaccag
    11401 acacgacgcg aagcttctta gaaagtctgc atttaccgca attcatcatt gataatatgt
    11461 atatcgaagg gatctatcgt caaccaacat ttctttatga atcactttgg agtttgattg
    11521 gatttatttt gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa ggcgaagtcc
    11581 ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt tattgaagga ttacgtacag
    11641 acagtcttta ctttttaggt gtgatccgta tttctcaatt attatctgct gtgttatttg
    11701 tcggtacatt gattctattt atttggcgca gaaaaaaagg aaatttaccg gattatgacc
    11761 gctcattagg aaaaaatcaa caaattatct gatttttaga gcgttaaaaa tgaaaaaaat
    11821 ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttggggtact
    11881 gctttggcac aagtcctcgc cgaaaacgga catgacgttc ggatttgggg acatcgtgct
    11941 tcacaagtag aagaaatcaa cacccaacac acgaatcaac gttatttacc agatcattat
    12001 ctacccgatt ccatccaagc attcaccaat atggaagatg cagtaaaaga cgcagatgcg
    12061 gtgctattcg tcattccaac gaaagcaata cgttctgttg caaaagaact agtgcctaaa
    12121 ttgaatacaa agccagtgat catccatgca agtaaaggat tagaacaggg aacacataaa
    12181 aggatctctg acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc tgttgttgtt
    12241 ctttctggac caagccatgc agaagaagta gcgaaacatg acatcactac gatcacagct
    12301 gcaagtacag atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa taattatttt
    12361 cggatctata ccaatcctga tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt
    12421 attgctattg gtgctggcgc tattcacggc ttagggtttg gcgataatgc aaaggcagca
    12481 atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg
    12541 ctgaccttta tcggattaag cggagtaggc gatctagtcg ttacttgcac aagtgttcat
    12601 tctaggaact ggcgcgcagg caacttgtta ggacaagggc agcccctaga agaggtgcta
    12661 gccaatatgg gaatggtcgt tgaaggtgtg aatactactc aagcagcgat ggaactagcc
    12721 caaataatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa
    12781 aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atgggaaaaa agaaaatgaa
    12841 ttctcattaa caattttttg aaggagcgaa tgtatatgaa agtcaaaaaa gcagttattc
    12901 cagcggcagg attaggaaca agatttttac cagcaacaaa agctatggca aaggaaatgt
    12961 tgccaattgt tgataaacca acaatccagt tcatcgttga agaagcgttg aaatcaggaa
    13021 tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt
    13081 caaatattga attagaaaca aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag
    13141 tagaagaaac gacagatgtc aacttgcatt tcatccgtca atctcatcca aagggtttag
    13201 gacatgccgt tcttcaagca agagccttta ttggaaatga accgtttgtc gttatgctag
    13261 gcgatgatat catggaagat gaagtaccat tgacacagca attaatcgat gattacgaac
    13321 gtactcatgc ttctacgatc gccgttatgc aagttcctca tgatgaaact tcaaaatacg
    13381 gaattatcaa tccaggagaa gtattggaag atgggttata caatgtcaaa aactttgttg
    13441 aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattggacgt tacttattga
    13501 ctccagaaat ttttgatgta ttgtccgagc aacaaccagg agccggaaat gaaatccaac
    13561 taactgacgc aatcgataca ttgaataaaa cacaacgcgt atttgcccgt aaattcacag
    13621 gtaaacgtta cgatgtcggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc
    13681 taacacatcc agaagtaggt gcacctttac gtgagtatat cctgtcttta ggtaaaaaat
    13741 tagaagcaga aggaagcaag atcgatcata atccaaaagg aataacagaa atcaaagaag
    13801 aaaagaaaaa ataacccttt tttctaacga gcttcagctg ctaattgaat aaaaagtagt
    13861 tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga
    13921 caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc agcaatcgcc
    13981 tttgcgatac ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa aactttagaa
    14041 aaagtagatc agactgtagc agaagcaaat acgacgatcg aaatcgtcac aaaagacgta
    14101 gatcttttgt ctagacaagt agaaggcttg ctggtcaaaa gtaatgaact gctcttggat
    14161 atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa
    14221 agtgtatcag aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg cggaatcggt
    14281 aaaacgacag ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat gaaatttttt
    14341 aaaaacaaaa aacatactga aacgacagga ggaaaataaa aatggctaaa aaaggtggat
    14401 ttttattagg tgcagtagtc ggaggaacag ccgcagcaat cgccgcgtta ttgttagcac
    14461 caaaatctgg taaagaactt cgggatgaat tagctgccca agcggatgat ttgatggata
    14521 tggcttcaga ttacacagat atggcgaaag aaaaatcgac tgagttaaca ggtattgcaa
    14581 aagaaaaagc gcaagatctt tctcaacaag caggtgatct agcaggtagt gtcaaacaaa
    14641 aagtaagtga atcagcagat gaagcaggaa gtgaatctga cgatatcttg ttaaatctta
    14701 aagaacaatc agctgactta tctaatcgtt tcaaaaaaac ggcagaagat atgaaaaaac
    14761 ctgcagatga attaggaagt attgctaaag atacttcaga agatatcatg attgatgtaa
    14821 aagacgctgc tggtaaagca ggagatactg tcaatgaagg aatgtcagaa gcaaaaccag
    14881 ttgcagatga cacaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa gaagctaaaa
    14941 aagaaataaa agatacaatg acagattcat ctacaaatca ctaaaatcac taaaatcaca
    15001 aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact cttatttttc
    15061 taaaactagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc ttgtcacata
    15121 tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg gttcaagaga
    15181 gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag ggaattcgtg
    15241 gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt agccatagct
    15301 gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg gacggacggc
    15361 ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtat attttttctt ggaagtcggt
    15421 tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc aaacaacgcc
    15481 aagatcgaat aacacaagtt cattcggtga aatcttcgtg tttcctggtg taccatgagg
    15541 gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg aaacaccttg
    15601 ttttttcaat tggtattcga tttctgcaat gatcgcttgt tcagtcacgc cttctttcac
    15661 tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga tcagtttgtc
    15721 tttttcctct agagttttga tcagttgtag acgttggatg atggaagcta gatcagtgta
    15781 agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag tcaaggcgtt
    15841 cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt ttccgatgat
    15901 ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac tactgttttg
    15961 agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa gagggataaa
    16021 aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag cgatgtggcc
    16081 agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac gtagttcatt
    16141 gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat tcaataattg
    16201 taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa aattttcatg
    16261 aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt ttagaaatgt
    16321 aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg gaaaagcaaa
    16381 caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact gtttcacgtg
    16441 tcgtaaatgg caatccaaac gtcaaaccag caacaagaaa aaaagtcttg gaagttatcg
    16501 atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag aaaacaacaa
    16561 cagttggggt aatcatccct gatgtcagca atatgttctt tgcttcttta gcacgtggta
    16621 tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca gatggtaacg
    16681 accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac ggcgtgatct
    16741 tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca aaaactcctg
    16801 ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac attgattatg
    16861 tatcagcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa aaaatcgctt
    16921 ttgtcagtgg tgcgttgatc gatcctatca atggacaaaa tcgtctgaaa ggctataaag
    16981 aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa gcacaataca
    17041 acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca acagctgtct
    17101 atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac cgtggcgtga
    17161 aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca gaagtagctc
    17221 gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta tctatgcgcc
    17281 tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc ttgccttatg
    17341 gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag gttgtgagcc
    17401 tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatttca tgtttgatga
    17461 atattgtgac tcgtgattcg gagttggcag gagaacaccg tttattcggg aaaaggggtt
    17521 atgacaaaat tttgtttaat cctttttttt tgtcttaaaa aagagagaaa gtggttgaaa
    17581 ttacatttat cgtatataaa acaatccgtg tatagatgct gcattatgtt caccaccatc
    17641 aaaaaaataa gggactgtga caatactatt gtcacagtcc cttattactg tgtattctat
    17701 taaatcaatc atctttctca tctgattctt tggttgcttg attcgctctt gcataagctg
    17761 aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca aatttaaagg
    17821 cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct ggtgtttgga
    17881 tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca gatacttttt
    17941 tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg tcaggttttg
    18001 cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc cttgctttgc
    18061 tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata gacggagtag
    18121 agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg acccaatctg
    18181 cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta gtgatttttg
    18241 cattgatcac actgcgcatc atgtcattca tgatcgatgc cgttgcttcc gaatagatcc
    18301 ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg atggaatcga
    18361 tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatcca ttggtctgtt
    18421 gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa ttgtcatttg
    18481 ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat ccaccggaat
    18541 tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct agtgactcac
    18601 ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc tcgccagcat
    18661 tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat ccgataagcc
    18721 ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca gcttgacgtt
    18781 ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca acgaatccgt
    18841 agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga ctgccagcac
    18901 catcatctaa catgtatcca taattttcta cgccattttg caatgcagca tagacatctt
    18961 tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt gccttttgat
    19021 aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca gccaattgtt
    19081 gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct ctgtcagtct
    19141 gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt gcttcttcgt
    19201 attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa tcttttctgg
    19261 caaggccggc agaaagatct tcttttagcg cacctgtgtt cgtgtatgga ctatacgtga
    19321 tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca tcttttgcat
    19381 ctacaccaaa gattcctctt gcagcttcct ggactccagc aatgttttgt cccttattat
    19441 tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt gaaaagtatt
    19501 tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt gtttcatctg
    19561 taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa gagccaatac
    19621 cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct tggtgtttat
    19681 cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa attttatcag
    19741 atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta ttatctgcat
    19801 aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct tttttagtcg
    19861 gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca atacctagag
    19921 ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa actctaaaaa
    19981 taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt cgagtatttt
    20041 tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata gcaaataata
    20101 gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa gagttaaaac
    20161 gtgagaaaat gatctttttt ataaaagagt tcttaagctt ttctttagag aaaatttcag
    20221 ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc atcttaccaa
    20281 tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat gttattaggc
    20341 tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga agcagccagt
    20401 tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag aagaaaagcc
    20461 atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaacgg tctaacaaat
    20521 atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc agaaatgttt
    20581 ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt aggcgctact
    20641 caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg aattgctaga
    20701 attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat cgctttggag
    20761 acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc gcaaatcatc
    20821 gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca tacgaatgat
    20881 gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga taagatcatc
    20941 gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca aggttcgcat
    21001 aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct gaataagatc
    21061 gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta tgtcggcaaa
    21121 gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa agcacaaata
    21181 ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta agattttcta
    21241 tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt taagaaacaa
    21301 aggagtgctt tttgcagtct ttttcatcat gcatcttctc ccccctcttt ttaaatttag
    21361 acaaacaggg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag caagtggcaa
    21421 aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc aaccaactaa
    21481 aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt attcgtttgt
    21541 atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat gaccagctgc
    21601 tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat acgactacaa
    21661 cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt
    21721 cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt
    21781 ttaaaccgat cgtgaagaac aagtatcttc ttttaagttt ctatttttat tttttgtttt
    21841 ctccgattag ttttacacat gaatattcca ctcgaacgta tcgtaatgcc ctcgtgattc
    21901 cggctgttga gatcattatc gcttgtttac tagctattta ttaccgtaaa tatagttcca
    21961 gcaaaaaaat gctgccgtgg tttttagggc tagctgtttc ttttcctttc ttttggttca
    22021 ttcgcgaaga ttccatctgg cttcttcctt ttactgtagt cgcgttaggc atcagtttcg
    22081 tccagatctt gctaggacaa gacttttctt tcaaagaagc aaagaagtta aaagaacgac
    22141 catttctcct ttcgaaacaa gaatggctga aactttcatt gtttttactc cctctagtcg
    22201 gtttctccat cgtctatcag tggatcgaat ataaaaacga gacaacatat agtattgccg
    22261 ccatcaatga ccgttcgagt ggcgaatttg gacgtttgat gaaacagctg atccgaatag
    22321 atgacgaaac gaacttaaat aaagaaaaca gtgagatatg ggtctcgcat gctgcattaa
    22381 ataaagcatt agacgcatct cctacatttc atgctgtttc tactcgtatt gacgaattgt
    22441 atacgactca tgcgtggacc caaggtggaa aagtaaaaga attaccagga gatatcattt
    22501 tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa
    22561 ccaatgattt ttggaaaaaa gtcaatcaag aattagaaga agcctatcaa aagggaacta
    22621 tcaaaaagaa aaaagaattc tatctgatgg gatcagggga tggaaagacg atgaatgatc
    22681 tgccaatcgt atttgctttt ctgaaatcag gatatgccta tgcatggcga tacaaagatt
    22741 tccatcaagg ggggaattat agtttcggta cgcaagaaca agtcgaacag gcccaagaat
    22801 tattgaatct tccactaatg gataattgga tcgatagcag tgatccgaga acacctgatt
    22861 tccagttgac aaaaacagca aaagtcgcga atgtcatgat aaaaatttac caagttctgg
    22921 ccattcccgt gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgcat
    22981 ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtagtcacc ggattaatac
    23041 tgacacaatt ggtttttctt ttcggcgtct cgtggttttg ttcttatgcg ccagaaaaaa
    23101 aggattattt tttaagtgtc tacactggcg caggcgtgcc attaacgcaa ttcatcgtag
    23161 taacaagcat gctagttttc agtaaattga atctgaaaaa agctttatct tccaaataaa
    23221 aacatactaa gattagagga gaacatcatt gacgaatgat gttctcctct attttctatt
    23281 tttagattat agtgaagagt gaaagaaagt tcgaacaaag ccttgaggac tatgagtccc
    23341 tatcaaaaac cgaacttctt tactgtcatt ttagaatcag gtttacgtat gttggggcca
    23401 tgagcccgtg tataggaaat tggaatcgaa atgaagcagt aaagttcttt caaaacttaa
    23461 aaaggaggaa aacaaatgcc ttacattctt gcattagatg tttcaatggg acacagctac
    23521 agtgtcctat acaaagacga cacctgctta tttgaagacg agatcgaaca cactcaaaga
    23581 ggatttcatg cattactcga agagatccag cacttgcctg aacgtccatt gattgttttt
    23641 gaagcaacgg ggatttattc cagaccagtc gaaaagttct ttcaagatca tcacttttct
    23701 tactgtttgt taaacccgtt agaagcgaaa aaacagatgg aagaaaacac cttatgcagt
    23761 tggaaaacag ataaatcgga tgcccatcgg ctcgcacaga ctcatcttaa aaatcaacgt
    23821 cagccaaaag aagtacaaag caatttttac ttagagatgc gcgatttagc ccgtttttat
    23881 caagaaattg agaagaaaat cactcgtctg cgcatggatt tacacaactg ccttcagttg
    23941 acttttccag agcttgaaca gttcttttta aacaggctga ccccttacgc attgatattg
    24001 atccgtcttt ttccccatcc agattttgta ttggcatcta ctcggacaaa aataaaaaat
    24061 aaactgatca acgaaacacg gaaaaaaatc tccgcgaatc gtgccgagca aaaggcagat
    24121 caaatcatcc actatgcaca gtgtgcctat ccagctgttg aaaaagacag tatccactgt
    24181 caaaaaacga tctattacgc tgaacttctc caagatttac tcgaacaaaa a
//
LOCUS       DAZBWR010000032        23830 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000032 DAZBWR010000000
VERSION     DAZBWR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.032"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACHGT5_001156"
     CDS             complement(54..788)
                     /locus_tag="ACHGT5_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN7217156.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACHGT5_001157"
     CDS             1115..1525
                     /locus_tag="ACHGT5_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN7217157.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACHGT5_001158"
     CDS             1518..2219
                     /locus_tag="ACHGT5_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN7217158.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACHGT5_001159"
     CDS             2219..3805
                     /locus_tag="ACHGT5_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217159.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACHGT5_001160"
     CDS             3824..4045
                     /locus_tag="ACHGT5_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN7217160.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACHGT5_001161"
     CDS             4042..4803
                     /locus_tag="ACHGT5_001161"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN7217161.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACHGT5_001162"
     CDS             5183..5692
                     /locus_tag="ACHGT5_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN7217162.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACHGT5_001163"
     CDS             complement(5767..6306)
                     /locus_tag="ACHGT5_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN7217163.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHGT5_001164"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHGT5_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN7217164.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACHGT5_001165"
     CDS             7319..8170
                     /locus_tag="ACHGT5_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN7217165.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACHGT5_001166"
     CDS             8198..8833
                     /locus_tag="ACHGT5_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN7217166.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACHGT5_001167"
     CDS             8853..9626
                     /locus_tag="ACHGT5_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7217167.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACHGT5_001168"
     CDS             10034..10831
                     /locus_tag="ACHGT5_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN7217168.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACHGT5_001169"
     CDS             10809..11222
                     /locus_tag="ACHGT5_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN7217169.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACHGT5_001170"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACHGT5_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN7217170.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACHGT5_001171"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACHGT5_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN7217171.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACHGT5_001172"
     CDS             15999..16559
                     /locus_tag="ACHGT5_001172"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN7217172.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHGT5_001173"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHGT5_001173"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN7217173.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACHGT5_001174"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACHGT5_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN7217174.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACHGT5_001175"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACHGT5_001175"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN7217175.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHGT5_001176"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHGT5_001176"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN7217176.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHGT5_001177"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHGT5_001177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN7217177.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHGT5_001178"
     CDS             21907..22485
                     /locus_tag="ACHGT5_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7217178.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHGT5_001179"
     CDS             22537..23019
                     /locus_tag="ACHGT5_001179"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN7217179.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHGT5_001180"
     CDS             23136..23510
                     /locus_tag="ACHGT5_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217180.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23623..>23830)
                     /locus_tag="ACHGT5_001181"
     CDS             complement(23623..>23830)
                     /locus_tag="ACHGT5_001181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217181.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7781 a         4286 c         5115 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact cctttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt
//
LOCUS       DAZBWR010000033        23757 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000033 DAZBWR010000000
VERSION     DAZBWR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.033"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACHGT5_001182"
     CDS             complement(228..1115)
                     /locus_tag="ACHGT5_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217182.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACHGT5_001183"
     CDS             complement(1112..1981)
                     /locus_tag="ACHGT5_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217183.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACHGT5_001184"
     CDS             complement(2118..3404)
                     /locus_tag="ACHGT5_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217184.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACHGT5_001185"
     CDS             3677..4255
                     /locus_tag="ACHGT5_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN7217185.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACHGT5_001186"
     CDS             4255..5184
                     /locus_tag="ACHGT5_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217186.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACHGT5_001187"
     CDS             5181..6917
                     /locus_tag="ACHGT5_001187"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN7217187.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHGT5_001188"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHGT5_001188"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN7217188.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACHGT5_001189"
     CDS             complement(7813..8322)
                     /locus_tag="ACHGT5_001189"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN7217189.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHGT5_001190"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHGT5_001190"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN7217190.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACHGT5_001191"
     CDS             complement(9623..10714)
                     /locus_tag="ACHGT5_001191"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN7217191.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHGT5_001192"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHGT5_001192"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN7217192.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHGT5_001193"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHGT5_001193"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN7217193.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHGT5_001194"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHGT5_001194"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN7217194.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="ACHGT5_001195"
     CDS             complement(14677..15651)
                     /locus_tag="ACHGT5_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN7217195.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="ACHGT5_001196"
     CDS             complement(15632..17515)
                     /locus_tag="ACHGT5_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN7217196.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="ACHGT5_001197"
     CDS             complement(17533..19758)
                     /locus_tag="ACHGT5_001197"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN7217197.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="ACHGT5_001198"
     CDS             19873..20907
                     /locus_tag="ACHGT5_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7217198.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="ACHGT5_001199"
     CDS             complement(20974..21987)
                     /locus_tag="ACHGT5_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN7217199.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="ACHGT5_001200"
     CDS             complement(22065..22253)
                     /locus_tag="ACHGT5_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN7217200.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            22800..>23757
                     /locus_tag="ACHGT5_001201"
     CDS             22800..>23757
                     /locus_tag="ACHGT5_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN7217201.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKKGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         6833 a         5052 c         4300 g         7572 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtagac taactaaaaa ggctctataa tttatgggac tgatgaatca gaattgattc
    22681 ttcagcccca tttttatttt aggtatcgcc gattgtaaaa ttaagctaga caactaaaaa
    22741 agtcatttgt gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtacaaa
    22801 tgacctatac ccatcttaca acggatgaac ttgtaataat agagtcttat ttcaaaatta
    22861 atcaatctgt tgctaaaact gcccattgct tgaatcgttc aagacaaacg atccataaag
    22921 tatatctgtt cttcaagaaa ggaaaatcag ccttagaata ttatcaacag tataagaaaa
    22981 acaaatcaaa ctgtggtaga cgtccgcttg ttttacccga ggaacaatca gaatatattc
    23041 aaagaaaggt tgttcaagga tggacacccg atgtgattgt tggtcgtgca gcgtttccta
    23101 ttagttgttc tgctcgtacc atttatcgta tgttcaaaaa ggggctattt gattcttctg
    23161 acttaccgat gaaaggcaag cgtaaaccga atggacatca agaaagacgt ggaaaacaaa
    23221 ctttccgccg ctctattcat gaacgtgaaa aggattatag ccaattctca aatgagtttg
    23281 gtcaccttga aggtgacact atcgtaggtc tgaaacataa aagtgctgta attaccttag
    23341 ttgaacgatt atcaaaagtt atcatcacat tgaaaccgtg tggtagacaa gcgattgata
    23401 ttgaaaaaaa attaaatcaa tggtttgaat ctgtaccgaa aaacctattc aaatccatca
    23461 cttttgattg tggaaaggaa ttttcaaatt ggaaacagat cagtaatgtc aatgatattg
    23521 ccatttattt cgctgatcca ggaacgccgt ctcaaagagg cctaaacgag aattctaacg
    23581 gattgttacg tagagatggt ttattgaaat ctatggattt caattcagta gatgaatttt
    23641 ttattcaatc tgtcgcatct aaacgaaata atattcctag aaaatcactg gattatcgaa
    23701 cacctttgga agtatttttg agttacgtaa gtattgatga tctgtctaac ttaattt
//
LOCUS       DAZBWR010000034        23707 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000034 DAZBWR010000000
VERSION     DAZBWR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.034"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..211)
                     /locus_tag="ACHGT5_001202"
     CDS             complement(62..211)
                     /locus_tag="ACHGT5_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217202.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1179..1505
                     /locus_tag="ACHGT5_001203"
     CDS             1179..1505
                     /locus_tag="ACHGT5_001203"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7217203.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1513..2421
                     /locus_tag="ACHGT5_001204"
     CDS             1513..2421
                     /locus_tag="ACHGT5_001204"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN7217204.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3047..3301
                     /locus_tag="ACHGT5_001205"
     CDS             3047..3301
                     /locus_tag="ACHGT5_001205"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFN7217205.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3843..4001
                     /locus_tag="ACHGT5_001206"
     CDS             3843..4001
                     /locus_tag="ACHGT5_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217206.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4073..5299
                     /locus_tag="ACHGT5_001207"
     CDS             4073..5299
                     /locus_tag="ACHGT5_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7217207.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5388..5780)
                     /gene="rpsI"
                     /locus_tag="ACHGT5_001208"
     CDS             complement(5388..5780)
                     /gene="rpsI"
                     /locus_tag="ACHGT5_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN7217208.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5796..6224)
                     /gene="rplM"
                     /locus_tag="ACHGT5_001209"
     CDS             complement(5796..6224)
                     /gene="rplM"
                     /locus_tag="ACHGT5_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN7217209.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6758..7225)
                     /locus_tag="ACHGT5_001210"
     CDS             complement(6758..7225)
                     /locus_tag="ACHGT5_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN7217210.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7345..8883)
                     /locus_tag="ACHGT5_001211"
     CDS             complement(7345..8883)
                     /locus_tag="ACHGT5_001211"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN7217211.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8963..9649
                     /locus_tag="ACHGT5_001212"
     CDS             8963..9649
                     /locus_tag="ACHGT5_001212"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN7217212.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9850..10377)
                     /gene="msrA"
                     /locus_tag="ACHGT5_001213"
     CDS             complement(9850..10377)
                     /gene="msrA"
                     /locus_tag="ACHGT5_001213"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN7217213.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10461..11138)
                     /gene="pgmB"
                     /locus_tag="ACHGT5_001214"
     CDS             complement(10461..11138)
                     /gene="pgmB"
                     /locus_tag="ACHGT5_001214"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN7217214.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11135..12263)
                     /locus_tag="ACHGT5_001215"
                     /pseudo
     CDS             complement(11135..12263)
                     /locus_tag="ACHGT5_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12263..14446)
                     /locus_tag="ACHGT5_001216"
     CDS             complement(12263..14446)
                     /locus_tag="ACHGT5_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN7217215.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14443..15468)
                     /locus_tag="ACHGT5_001217"
     CDS             complement(14443..15468)
                     /locus_tag="ACHGT5_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN7217216.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15513..16340)
                     /locus_tag="ACHGT5_001218"
     CDS             complement(15513..16340)
                     /locus_tag="ACHGT5_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN7217217.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16337..17404)
                     /locus_tag="ACHGT5_001219"
     CDS             complement(16337..17404)
                     /locus_tag="ACHGT5_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN7217218.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17430..18266)
                     /locus_tag="ACHGT5_001220"
     CDS             complement(17430..18266)
                     /locus_tag="ACHGT5_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217219.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18263..19132)
                     /locus_tag="ACHGT5_001221"
     CDS             complement(18263..19132)
                     /locus_tag="ACHGT5_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217220.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19202..20485)
                     /locus_tag="ACHGT5_001222"
     CDS             complement(19202..20485)
                     /locus_tag="ACHGT5_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217221.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20499..22184)
                     /locus_tag="ACHGT5_001223"
     CDS             complement(20499..22184)
                     /locus_tag="ACHGT5_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN7217222.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22459..23484
                     /locus_tag="ACHGT5_001224"
     CDS             22459..23484
                     /locus_tag="ACHGT5_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7217223.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         6890 a         4940 c         4270 g         7607 t
ORIGIN      
        1 aaatataaaa ggtacgggtt actgattact tgtaaatctt tacgttccag ttttattctt
       61 tctaaaaaat atactcactt cttttcttag acttcttttt ttgattctcc tctttatggt
      121 cttgtccttt tttgccttca aaatatttaa ataaacccct ttcaaaagca tttctaggtt
      181 ttggacaata aggaatgact tttttcttca tataaatacc tcatttaact atttgatatc
      241 atcacattat caaaaaaaat aggtttttta ttaaaactat cttgaccttt cgggagattt
      301 cactgtgatt tatttttatt cccatacttt tttcgttggt cactttcgta tacctaatct
      361 aagcgatcat cggcgacggt gcttttacaa cgaatggcag gacaaaacaa ggggcggaaa
      421 aacttttttg cctatttcat aaaaaacttt ttcctacagc aatcccttgt tttgtcctac
      481 cacacgttgt gaacccaaag cacgccgacg aacgcttagc tatacgaaaa tgacctccaa
      541 aaacaaaaaa gtgaggtgaa tagaaacaaa aagaaattga aatctcccaa aatgtcgtcc
      601 gcctcggaga caacttgctg ctgaaaatgg ttggttaatt catttaagta ttaaataaga
      661 taaatcatca aattaatcgg aaaaatagca tgattggcct tatcaaaaat aaactgtaaa
      721 aacttatatt tctatgagtg gtggaacaat gctaatacca aattaattta tcgattaccc
      781 acctgcacaa accaaagata gcattattag gaaacgaatc aatcagcgaa tccagcatta
      841 taatgaggat actaaaaata aagttttcat aattataaat ccgccttcct acgaaaggca
      901 ccaatgaggt gatgaaaacc ttagagcgct ttctttcttt ttttattccg tgttaccctt
      961 tccttgcagg tccctggtac actacagaac ccgaggaggt gagtggcggg gctgtatagc
     1021 ggcaaccccg cataattata tggtgtcggt catccgttaa ggcatcaatg aatggcgcaa
     1081 acccgtagcc cgtaaactta tctcttccga agttgactcg atttcgccgg atgaccagtc
     1141 cctaccaaac ctgtactatt tttttacagg aggttgctat gttcaaaatc tttcgtaaaa
     1201 attgttgtgg cttggatgtc cacaaaacgt ggatctatgc gtgtatcgga atcacagatg
     1261 cgaatggtcg tacagtgtat aagcaagctc gcttctcttc ctttacaaaa ggtctcaatg
     1321 aattagctga atggcttgct aaatacaact gtaaggatgt gtgtttggaa tcttccggta
     1381 aattttggat tcctgttttt aatattcttg aaaaaacttg ttttgtcaca ctggctcatc
     1441 cgaagtacac caaaccccag aaaggaaata aaacagatcg caaagatgca aaaatggatt
     1501 tgtgatctct ttatgtgcga catgatcaaa cccagtttta ttcctggtct tgaaatccgc
     1561 cagcttcgtg atctaatgcg ttatcggacg aaactgacca acatgaaaac aagtgaaaag
     1621 aatcgtgctt taaactgctt aaccgtttct aatctgaaac tagatgatgt ttttagtgat
     1681 gtgttcggaa aatcatccac ttctatcatc caatacttgt tagaacatcc cggtgaatca
     1741 tttgatgttc gaccatttat ccatgcccgt tgtaaaactc ccgttgaaga aatccaagcg
     1801 gcagttgagg gggcgatttc tgtacctcaa gcgattaagc ttcgccaatg cctcaaccat
     1861 attaacgaga ttgagaagca tcgagaagag atcaatctag agattcaagc tcttgcagaa
     1921 cccttctccc ctgttttgga actacttcgt accataccgg gcttagacac tcagccaata
     1981 acagcgattg cgattctttc agaaatagga ccggatatgt ccgtgtttcc aacttcgaaa
     2041 cactttattt cttgggctgg atgctgtcca agaaacgatc gcagtgcccg acgaacaaaa
     2101 tcaacacgta tctctcgtgc agggcagtcg ttgaaaccgc tacttgtaca gattgccaat
     2161 gctcttctca gatccaaaga acatccagaa tttaaaaatc gctatcgtag aatcaaagcg
     2221 aatcgtggac acaagaaagc tatcattgct atttgtaaga tgctcctgac cgctatctgg
     2281 aatgtcttat caaaggtgga accctattct gctaaaggat atctggagcc aaaacctgcg
     2341 aaagaagaga aagttctgac gcaatcccaa gctctggacc ttttaagaaa acgcggatat
     2401 accattatag atagtgctta aaccaaagtt acgttgattc ttcctaaata gactcgttgt
     2461 cttccatttc aaaaattttg aaaacggtag gcttatttag tgtaccgttc ttcaggtatc
     2521 ttcaaataga tttattttca aacttatttc ctttcttctt ttactctcct atagcttagc
     2581 atttaagtgg gcactcgtgt caccttgcta gatagctatg aatgccgtaa taagcctcct
     2641 agtatatagg cagactgcca attataactg attcaatcca cattatgata aaatccacct
     2701 tttcgtttca tatgtgttga aatatcacat atgtcaaaag tgcacgagtc ttttcattca
     2761 gtgctttgta aatactcaaa attattcctt ttcactttaa aaaaaagata attagtacaa
     2821 aatcagaaat gaggaaagta ttaggatgga ctagaaagga gttgttgaat ttgttgcaac
     2881 agattggtca tttatcaatt aaaaattagg tgaagtttta ttcatcactg cagctgctgt
     2941 ttcccagaga ataaaacgtc tagaagaaac tggtcagtta ctgtattatc aagcagcaat
     3001 tgactacgaa caagcttgat tactaaccaa agcgtttatc cacctgttga ttaaatcaaa
     3061 tcaaaaatca ggtttttata cgttcataaa agcaatctcc agtgtactcg aatgtgactg
     3121 tatcataggt ccttattcga tgttaatcaa aatggggttt ccttctaccg aatcattgga
     3181 cagttttatc aatgatttat caaagtttgg acgaaccaac acccaaatcg ttttctcgac
     3241 tcttgtttcc ttaatggttt atcagttctg gaatgtttat catgcgcttt tattaaaata
     3301 gaaaagagct tttttcatgc gacaaagcag tgatcattta ttggcacatg caatctttgt
     3361 ggatccattt tttgaaaata cgatatttag ttttgaccca aaaatcttta aatacgtatt
     3421 tgataattta tcttaacaac cagataattt tattcagcac cgtattatct atattgaatt
     3481 ctcggaatca agataaattt ttttccgttt ttgtttcttt tttaatcttc ttcttgttat
     3541 taatagtgat taggatgata ttcctatatt gatctttgat aattagatat aatttttgaa
     3601 taacgttccc tgctattcga gctagaaata caagtacgcc atgacctagt tagcgacaac
     3661 tacttagaga tttcttttgt tctgggacaa caaaatgcca tgacaatagc agtaaataat
     3721 gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca
     3781 aagcggttca aaaattttaa actattattt taggagatga ttttataaaa tcgaaagcct
     3841 acttgaataa aaaacaagcc tgtgaatttc taggaattga taataatact ttagataagt
     3901 ggattcgtgt atataatttt ccagtaataa aaattgatgg cgtagttcgc tttgacgtag
     3961 aggatatcaa agatttcatg aatacattca aaaaaaattg aaattttctt ctcgtcacat
     4021 atactataaa tagcgaaatg tactgcctta ctttttaaag gagtgaggaa gtttgcctac
     4081 aaagctttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat tcagagaaat
     4141 cactggaatt gcatcagaga agtatcaaaa agtatttcca actaggcaat tagctattaa
     4201 ggccgaaagg gaaatgaaaa agaagattga aacagtttta agagaggaga atgcaaactc
     4261 actagaattg aaaggaaaaa taaaatttaa agatttttat gaatcaaaat ggcttcctag
     4321 atatgaacta ggtcaaacta ctaggtcaaa cagggctcca agtaatgtaa caattagtaa
     4381 tactaaggat atattccgat tgcacatcct tccaatgttt ggtgaatatg caatgaatta
     4441 tctgaattca aatactgaga taatatcaga tgaattaaca aagaaatcga aagaatacgc
     4501 aaatataaaa attatcaaag gctatgttcg ctctatgttt gatattgccg aaatactatg
     4561 ttatatcgaa ttcaaccgaa ctaccaaaat tattcaatgt attaccgcgc ctaagaaaat
     4621 ggctttagaa gaaaaaagga taagggaagg gaatcaagca ttatcatcag aagagctgac
     4681 tgattggata gaagcagtaa acaatgactt aaataatcac ttgcttacac ttcacgatta
     4741 cacacttttc atgcttacct tatatctagg tgatagaaaa agtgagactt atgctctgca
     4801 atggaaatat attgatttta ataaaaatac ggttcgtctc aaatatgctt tagataaata
     4861 tcaaagaaag acacatacaa aaggttggaa agacaccgtt attcaggcac cagaagttgt
     4921 gatgacatta ttaagagaat ggaaatcagc tcaggctgaa caattgttga aactaaaaat
     4981 tatacaaaca cctgaccagt acctctttac ctattccaaa ccttctggag aagtaaattg
     5041 tcctgttcac gctgactact taaactatcg aatcaatgca ataaaaaaaa ggcatcctga
     5101 attggctcaa ttatctccac acaaacttcg tcatacctat gcaactatag ctagggaagg
     5161 tggagcaaat atgaatcaaa tctctaatgc tttaacgcac tcggatattt ctactacaaa
     5221 aatatacgtg aacacacctg acgtagttga taaatctgtt tttgaagcat ttcaaaaagg
     5281 attgaataag tgtgattaac atgtgttctc aaatgtgttc ttattataaa aaaagacact
     5341 ttccaaataa ttgaaaagtg tcctgaaata ttgatataaa aagaatatta acgttttgag
     5401 aattgtgaag ctttacgagc ttttttaaga cctggttttt tacgttcgac catacgtgcg
     5461 tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc
     5521 aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt
     5581 acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt
     5641 acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt
     5701 ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg
     5761 atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa
     5821 acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta
     5881 aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa
     5941 cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag
     6001 ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgctaattt cactttgtct
     6061 gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt
     6121 ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg
     6181 tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg
     6241 ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt
     6301 tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt
     6361 tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg
     6421 gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc
     6481 cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat
     6541 aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct
     6601 tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
     6661 caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt
     6721 tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt
     6781 tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa
     6841 agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa
     6901 gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat
     6961 cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat
     7021 aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat
     7081 catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc
     7141 aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata
     7201 cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa
     7261 taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt
     7321 tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg
     7381 agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat
     7441 tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa
     7501 ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc
     7561 tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc
     7621 catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag
     7681 ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag
     7741 aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa
     7801 cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc
     7861 attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc
     7921 tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat
     7981 gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca
     8041 aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct
     8101 cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag
     8161 cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc
     8221 tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc
     8281 tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa
     8341 atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca atctataaaa
     8401 gatatagtct ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt
     8461 ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg
     8521 ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt
     8581 atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc
     8641 atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat
     8701 tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat
     8761 cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc
     8821 atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt
     8881 cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg
     8941 gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc
     9001 atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc
     9061 tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat
     9121 catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt
     9181 ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa
     9241 tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta
     9301 cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt
     9361 gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg
     9421 ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag
     9481 tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat
     9541 atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat
     9601 tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt
     9661 gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt
     9721 ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca
     9781 gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt
     9841 ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc
     9901 ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc
     9961 tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc
    10021 gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata
    10081 gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg
    10141 atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata
    10201 ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg
    10261 atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc
    10321 aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata
    10381 aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt
    10441 gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa
    10501 cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg
    10561 ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct
    10621 gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga
    10681 aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa
    10741 taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat
    10801 ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg
    10861 gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag
    10921 gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct
    10981 agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg
    11041 gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga
    11101 acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa
    11161 cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct
    11221 gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa
    11281 catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc
    11341 cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta
    11401 gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtatcgaa ctccagtaaa
    11461 tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca
    11521 cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata
    11581 taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt
    11641 ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg
    11701 acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt
    11761 ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa
    11821 cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg
    11881 atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg
    11941 gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc
    12001 atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat
    12061 gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc
    12121 gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct
    12181 tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa
    12241 tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca
    12301 gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta
    12361 ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag
    12421 aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg
    12481 taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa
    12541 ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata
    12601 aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac
    12661 tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg
    12721 gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga
    12781 cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac
    12841 gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa
    12901 tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt
    12961 tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca
    13021 gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc
    13081 tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt
    13141 catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat
    13201 actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa
    13261 cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac
    13321 cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt
    13381 gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa
    13441 actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat
    13501 aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca
    13561 taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt
    13621 ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa
    13681 tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt
    13741 gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga
    13801 tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat
    13861 tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga
    13921 tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg
    13981 tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga
    14041 tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga
    14101 cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt
    14161 ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac
    14221 cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg
    14281 ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt
    14341 cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat
    14401 acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc
    14461 tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca
    14521 gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact
    14581 tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac
    14641 atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac
    14701 attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc
    14761 gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca
    14821 tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac
    14881 gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata
    14941 atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac
    15001 accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg
    15061 attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg
    15121 cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg
    15181 tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa
    15241 gcgattggct gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac
    15301 tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac
    15361 tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg
    15421 tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt atttctctct
    15481 cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga
    15541 cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca
    15601 ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc
    15661 gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct
    15721 gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct
    15781 tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag
    15841 tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa
    15901 ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat
    15961 tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga
    16021 taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt
    16081 gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca
    16141 ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt
    16201 gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca
    16261 aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga
    16321 tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt
    16381 cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa
    16441 ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt
    16501 cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag
    16561 caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa
    16621 aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct cttaaagaag
    16681 cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca cgtttatctg
    16741 tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg taatcggcac
    16801 cgtatttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg atggtagtag
    16861 cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg
    16921 ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg aattgagcag
    16981 gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt agtttataat
    17041 tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag actaactctc
    17101 ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca atcatatttc
    17161 ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt ggcaagaata
    17221 ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta aagtcgacaa
    17281 tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg ttcttttcga
    17341 tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca actaattttt
    17401 tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc gactaaatca
    17461 cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc ggtcaccaat
    17521 gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg
    17581 attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa ataatcattc
    17641 cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc caatggtata
    17701 atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc ttcttcaata
    17761 tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg aagtgtttga
    17821 acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc ggaaatgatt
    17881 ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta aaagctagca
    17941 ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc gctgatccca
    18001 atcactactg cagttacgga ggctatcaag gagatcacta aactattttt gaaatacatg
    18061 atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt ccattctttc
    18121 ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc
    18181 atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc gtaaaataag
    18241 attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag agcaagacga
    18301 ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca agatcattga
    18361 acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc gtcagtagat
    18421 agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca gttgctagta
    18481 ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt gcaccgtcca
    18541 tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa
    18601 acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag ctagcaactg
    18661 gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact aacagatcat
    18721 tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa gaaatagatg
    18781 gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg aacttgcggt
    18841 taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact aagaatgtgt
    18901 aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt tcaagataat
    18961 tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg aaaaaactga
    19021 tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc atgcttggtg
    19081 ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc attgaaacct
    19141 ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc agaacttctt
    19201 gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg tttccgacag
    19261 ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc attttcgtga
    19321 aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta gcaatctcag
    19381 atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc agttcgacta
    19441 cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt tccatgtttt
    19501 tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc tcgtctagac
    19561 cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt tgtgttggga
    19621 tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa atgtaagttg
    19681 aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct gtcacatcat
    19741 tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct tctctcatct
    19801 tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca tttgacaaag
    19861 caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata gcaattccat
    19921 attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca tcccaatttt
    19981 ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata ccttgaaccc
    20041 agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca aggtttttgg
    20101 ctccgtcata ataattatct tctccagctt cctcgataac gttttggact gccgtctgat
    20161 caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc attacttctg
    20221 gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat tcttctaccg
    20281 gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc agttcattga
    20341 tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg
    20401 attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta gctagcagac
    20461 aggtagtaac aataattttt ttcatttttt tccacctctt attttattat tttcagccac
    20521 atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat aatttgtcca
    20581 gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg attggaaaaa
    20641 ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat aaaagaatct
    20701 gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt ttttcgaata
    20761 gatagcatct gggtcaattc accatatact gctgttctta atggatctgt ctgcaactct
    20821 ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc tcctgttttt
    20881 tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat tgccggcacg
    20941 cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct ccattccgtc
    21001 gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata tggactggtc
    21061 gttccatctg ggtttttctt gtagtttact cgggcaccct ctttcttcag ctgttccact
    21121 aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc gataccgtca
    21181 tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac
    21241 cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa aggtggtagc
    21301 gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact gatattatct
    21361 ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc atcagcgatt
    21421 gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga agttcctagt
    21481 tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt ttctaagtaa
    21541 aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt caagtctact
    21601 tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt
    21661 gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc cagtggcaaa
    21721 tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa ccaagtactt
    21781 tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct tctcatttct
    21841 tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac aacggagaaa
    21901 ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa tgtatctgcc
    21961 aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat
    22021 tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg
    22081 cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt tttctcagac
    22141 aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt cccctttctt
    22201 ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg tcaatagcat
    22261 ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat
    22321 taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca
    22381 aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa
    22441 aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc
    22501 tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa
    22561 gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga
    22621 ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc
    22681 tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat
    22741 aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg
    22801 aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa
    22861 acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg aaaagtcgga
    22921 aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg
    22981 gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa
    23041 gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta
    23101 gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca
    23161 atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta
    23221 aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt
    23281 tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg
    23341 atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc
    23401 caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat
    23461 tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa
    23521 gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta
    23581 gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt
    23641 taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt
    23701 tatagtt
//
LOCUS       DAZBWR010000035        23653 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000035 DAZBWR010000000
VERSION     DAZBWR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.035"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACHGT5_001225"
     CDS             complement(26..601)
                     /locus_tag="ACHGT5_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN7217224.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACHGT5_001226"
     CDS             complement(606..1580)
                     /locus_tag="ACHGT5_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFN7217225.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACHGT5_001227"
     CDS             1863..2831
                     /locus_tag="ACHGT5_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN7217226.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACHGT5_001228"
     CDS             2928..3221
                     /locus_tag="ACHGT5_001228"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7217227.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACHGT5_001229"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACHGT5_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN7217228.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACHGT5_001230"
     CDS             3678..4433
                     /locus_tag="ACHGT5_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN7217229.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACHGT5_001231"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACHGT5_001231"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN7217230.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACHGT5_001232"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACHGT5_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN7217231.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACHGT5_001233"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACHGT5_001233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN7217232.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACHGT5_001234"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACHGT5_001234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN7217233.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACHGT5_001235"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACHGT5_001235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN7217234.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACHGT5_001236"
     CDS             9170..9712
                     /locus_tag="ACHGT5_001236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN7217235.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACHGT5_001237"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACHGT5_001237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN7217236.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACHGT5_001238"
     CDS             11305..12207
                     /locus_tag="ACHGT5_001238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN7217237.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACHGT5_001239"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACHGT5_001239"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN7217238.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACHGT5_001240"
     CDS             13650..14072
                     /locus_tag="ACHGT5_001240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN7217239.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACHGT5_001241"
     CDS             14541..14771
                     /locus_tag="ACHGT5_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN7217240.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACHGT5_001242"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACHGT5_001242"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN7217241.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACHGT5_001243"
     CDS             16182..16358
                     /locus_tag="ACHGT5_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN7217242.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACHGT5_001244"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACHGT5_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN7217243.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACHGT5_001245"
     CDS             16853..17320
                     /locus_tag="ACHGT5_001245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7217244.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACHGT5_001246"
     CDS             17405..18289
                     /locus_tag="ACHGT5_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN7217245.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACHGT5_001247"
     CDS             18403..18639
                     /locus_tag="ACHGT5_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN7217246.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACHGT5_001248"
     tRNA            18727..18807
                     /locus_tag="ACHGT5_001248"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACHGT5_001249"
     tRNA            18814..18885
                     /locus_tag="ACHGT5_001249"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACHGT5_001250"
     CDS             19011..19181
                     /locus_tag="ACHGT5_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217247.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACHGT5_001251"
     CDS             19401..20108
                     /locus_tag="ACHGT5_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7217248.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACHGT5_001252"
     CDS             complement(20167..20811)
                     /locus_tag="ACHGT5_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN7217249.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACHGT5_001253"
     CDS             20938..21225
                     /locus_tag="ACHGT5_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217250.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACHGT5_001254"
     CDS             21333..21653
                     /locus_tag="ACHGT5_001254"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7217251.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACHGT5_001255"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACHGT5_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23463
                     /locus_tag="ACHGT5_001256"
     CDS             23044..23463
                     /locus_tag="ACHGT5_001256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217252.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DAZBWR010000036        23251 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000036 DAZBWR010000000
VERSION     DAZBWR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.036"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACHGT5_001257"
     CDS             <1..680
                     /locus_tag="ACHGT5_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN7217253.1"
                     /translation="NVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWV
                     IISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIY
                     FASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFY
                     NIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRF
                     DSDSALF"
     gene            692..1612
                     /locus_tag="ACHGT5_001258"
     CDS             692..1612
                     /locus_tag="ACHGT5_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217254.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1653..3110
                     /locus_tag="ACHGT5_001259"
     CDS             1653..3110
                     /locus_tag="ACHGT5_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217255.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3455..4069
                     /locus_tag="ACHGT5_001260"
     CDS             3455..4069
                     /locus_tag="ACHGT5_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN7217256.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4062..5786
                     /locus_tag="ACHGT5_001261"
     CDS             4062..5786
                     /locus_tag="ACHGT5_001261"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN7217257.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5783..7234
                     /locus_tag="ACHGT5_001262"
     CDS             5783..7234
                     /locus_tag="ACHGT5_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN7217258.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7706..9859)
                     /locus_tag="ACHGT5_001263"
     CDS             complement(7706..9859)
                     /locus_tag="ACHGT5_001263"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN7217259.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9861..10907)
                     /locus_tag="ACHGT5_001264"
     CDS             complement(9861..10907)
                     /locus_tag="ACHGT5_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7217260.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11057..12346
                     /locus_tag="ACHGT5_001265"
     CDS             11057..12346
                     /locus_tag="ACHGT5_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN7217261.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12359..15061
                     /locus_tag="ACHGT5_001266"
     CDS             12359..15061
                     /locus_tag="ACHGT5_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN7217262.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15046..15903
                     /locus_tag="ACHGT5_001267"
     CDS             15046..15903
                     /locus_tag="ACHGT5_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN7217263.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15974..16831)
                     /locus_tag="ACHGT5_001268"
     CDS             complement(15974..16831)
                     /locus_tag="ACHGT5_001268"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN7217264.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17042..19213
                     /locus_tag="ACHGT5_001269"
     CDS             17042..19213
                     /locus_tag="ACHGT5_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN7217265.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19281..19622
                     /locus_tag="ACHGT5_001270"
     CDS             19281..19622
                     /locus_tag="ACHGT5_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN7217266.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19898..21652)
                     /locus_tag="ACHGT5_001271"
     CDS             complement(19898..21652)
                     /locus_tag="ACHGT5_001271"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217267.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21663..22499)
                     /locus_tag="ACHGT5_001272"
     CDS             complement(21663..22499)
                     /locus_tag="ACHGT5_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN7217268.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22501..23184)
                     /locus_tag="ACHGT5_001273"
     CDS             complement(22501..23184)
                     /locus_tag="ACHGT5_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN7217269.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7641 a         4158 c         4783 g         6669 t
ORIGIN      
        1 ataatgtagc atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga
       61 gcgagttgag aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt
      121 ttctatcttg ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa
      181 tattcaatca gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga
      241 aatattggcc gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca
      301 ttatttactt tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg
      361 atggagctag caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa
      421 tgacgatttt aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt
      481 ataacattcc taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata
      541 tctacaatgg cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc
      601 aatcagttgt cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata
      661 gcgattctgc attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca
      721 agtccgctct ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc
      781 tatttcctgt attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat
      841 attgtcgact cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta
      901 cttgttcgac cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac
      961 tgttttaggt acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg
     1021 gtcaaacttt ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc
     1081 accagggatg gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat
     1141 ttgggcattg atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt
     1201 ctttaaacgt tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga
     1261 actgcgaata tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag
     1321 tttatttgcc gcattgggtt actggaatga ctggttcaat gctttactct acatccaaga
     1381 tgataagcta gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct
     1441 gacacaaaat tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga
     1501 atctgccaga atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt
     1561 tttccagaag cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa
     1621 aaatataaat cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta
     1681 caggtgtttc agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag
     1741 accaggcaga agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag
     1801 ataatttcga tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa
     1861 aagtcaatat caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt
     1921 cttccgggga aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag
     1981 gagcctttgc tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt
     2041 tagatgaagc atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca
     2101 atggaaatgt ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg
     2161 atctttcaat tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc
     2221 atgaaaaaga accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga
     2281 actttgatta tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt
     2341 cgaaaatcat caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata
     2401 gctggtacaa acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac
     2461 tagaaggaaa tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata
     2521 caattttgac aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa
     2581 aatctacagc tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag
     2641 aaaaatcagt agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg
     2701 tttgggggat cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat
     2761 tgctagatgg ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga
     2821 ttctatatac acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg
     2881 ctgatgcaga aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag
     2941 aattaagtaa tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg
     3001 ttgatccaga tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata
     3061 aggtattgaa agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa
     3121 agacaaaggg gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc
     3181 aaaagccagt ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac
     3241 aaaatttttc ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt
     3301 tcaatcagct gaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc
     3361 cgtttgtttc tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg
     3421 agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt
     3481 atttatccgg atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc
     3541 cggcgggatc atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt
     3601 gtacggattc gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa
     3661 ttttttaaga ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta
     3721 caatttatat ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt
     3781 actttttgcc ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga
     3841 atatacgatc tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt
     3901 ttcgtctttt ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt
     3961 caaaggattg atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac
     4021 aacacattgg cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc
     4081 tattttcaaa aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg
     4141 gtgtaacgat gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac
     4201 aggcagaaaa catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa
     4261 caaccttgag taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg
     4321 tgacgaccta cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa
     4381 acagtaccga tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg
     4441 aagaactaag tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta
     4501 gagaaaacaa aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata
     4561 tcaagttgcc tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa
     4621 agaaagaact agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca
     4681 tgatttctgg aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa
     4741 cgtttgaaag acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga
     4801 ttgacgatct ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta
     4861 tattagctgt actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc
     4921 tgatcattgg tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca
     4981 aacgatattc acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga
     5041 atttagagac gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg
     5101 gtatcaacga aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa
     5161 tcaaacagca agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat
     5221 ataatacatt agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag
     5281 atgttgtgta tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca
     5341 ctttaaaaga agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc
     5401 ctaatcgagt agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta
     5461 aatttgtgat ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat
     5521 tcgataatgc gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca
     5581 aagataatgg aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc
     5641 atcctaaagt ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg
     5701 ccagcttcgg cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg
     5761 tttctattac attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata
     5821 catgattttg aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt
     5881 agcaacagca gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt
     5941 agtagtaacg gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca
     6001 aaaagaacgt cataattttg aatttatgat tttatcagga taccaagaat tcgattattt
     6061 gaaaggaggt atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga
     6121 attggttgaa agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa
     6181 tcaacaagaa atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga
     6241 aataagtgaa gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat
     6301 tcaactggca agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat
     6361 cttttactat caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga
     6421 tgaagttgat ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat
     6481 cagtatcggg gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa
     6541 agacaatcta cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt
     6601 acaagcagaa acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa
     6661 taaacaattg gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc
     6721 agccatgatg ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg
     6781 ggaattgatt gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc
     6841 aaaaacggtg caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca
     6901 gaaaaagtac agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga
     6961 accacttaca ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca
     7021 gctgttcaaa aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga
     7081 aaaagcgaaa caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat
     7141 tggctacaac aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc
     7201 taaagagtac cgagaaaaca gtggagaatc ataaaaaaaa agccaatgta atgaaataac
     7261 ttataaaaaa ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag
     7321 ctttttcatg acctctcgat aaacggagtt caaaagccag tttctcggta ttaagccaaa
     7381 tttatgcaaa aatgtggaaa gccattttca caaaattttt cttaatactc gaaactaaac
     7441 agctttttca cgacctctcc actaaatggt gttcaatcag ctgaccctcg gtattagctc
     7501 aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga
     7561 acgctgattt cacaacctct caataaacgg tgttcaaaag cttgcatctg cggtaataag
     7621 accaaccaaa cgaaaaatat gaggatctat ttttgcttgg ctgttcttat tacttgatgc
     7681 aggacagctt tttcacaacc tcttgttaat tatagtttga taaggaaaaa ggcaaatctt
     7741 ttgcttgata tttcttaggg tgagggatga tccctaattg gtaagagatc gtgccgcctt
     7801 tcagcaaatc ttgatgggta aaatataatt tcgtatattc tctgtccatc cgtttgattt
     7861 tatggataaa ttgttgctgc ggccgattag gcgttgcttc aatcgtcaat tcctttccat
     7921 ttgacaaaag caaaacagcc ttgtcaatta atggcatacc gataacatat tcaccggttc
     7981 caggcgtgac aggataaaat cccagactat tgaagatata ccaagcggcc atacttccat
     8041 tgtcctcatc tccagggtaa cccattggag aatcgtcgaa ggtctgagtc atcaactgtt
     8101 taagcagtgg ctgtgccatt tcaggttgtc ctatataact gaacaagtaa ggatagtgaa
     8161 aacttggctg gtttgaaata gctaattgtc caaattctat cgcggccatc tcactcattt
     8221 cgtggatctc aaaaccatat cctcctgggt caaacgttgg gggctgattg cacagctcaa
     8281 tgagtttttc tttatatctc tcactgccac catattttcc gatcagtcct gaaaaatcgt
     8341 gataaacagc aaagctgctt tgccaagcac ttccttctgc ataatcttgt ccccaacgat
     8401 tagctgaaaa tggttctctg aacttgccat ctttatcttt tgaacgcata aatcctgact
     8461 catagtcaaa gatattttga tagttcaatg attgctgctg atatttcttc gtcatttcct
     8521 cttcttctag taccttggca acttggctaa tacaaaaatc gctataagca tagtctaatg
     8581 tgtgattgac tgattcgtgg tagtgattag gaacatagcc atatgtcaag taatcttctg
     8641 ttccttggcg tccataattt ggatgtttgc tttgaaccgt agcacctttt tccattgccg
     8701 ttagaaattc aggcatcaag tctcgtcgga tatcttttac agcagcatct gcaatgaccg
     8761 catcgatcaa tgtcccaggc ataagtccac gttcatcagg agacagccat tttggcaaat
     8821 agccactttc tctatagctg ttcaaaaaac cttccagcat ttcttcgtat ttttctacag
     8881 caatcaaaga ataaagagga taaaccgttt tatacgtatc ccaaaaccca ttatttgtat
     8941 agagaacacc aggttttata gatcgactag tcgtatcata gtggattttt tgcccactag
     9001 gatcaatttc ataaaatgtt tgtggaaata aaaacacccg atataaatta tgataaaacg
     9061 tccgcaattg ttgttgatca tggtgtgtga gctgtatacg atcaaaatag gactgccatt
     9121 gttcttgact gtgagcaagg taagtctctt tgctttggtt ttgttctagt tccaggttat
     9181 aagctgcttg ttccgagctg ataaaagatg tcccaaatcg gatagtttgt ttttttgtct
     9241 gttcaaaata caacgggatc gagtcgctat ttttgttgga aacagctaac tcatcaattg
     9301 ttaaaggata ttcaaatctc aatataaaat aaaaagaaaa atcaggatct tctgaaccag
     9361 caaaatgtgt gatttttcct tccactgtgt aaggatctat aacaaagagc tgatggcgtc
     9421 ccggaaagga taaaagtaaa ccgctgtcat ttttgctgta atcaatagat aagattccgc
     9481 catacatgct aggtatcaat gtagaacaga tgccataacg taattgacag atcgacagat
     9541 gagtcgggca aaatatactt tcttctgggc ggtaagaact ttgagcatgg aaaagggtgt
     9601 tctcctgtag ttttccattg atcggcgtaa acagcatatg actgaaatca cccatccaag
     9661 gacttggttg atgggtaaga cggtagcctt gaaaaacgcg gtcatcagga tgaaaccacc
     9721 agctgccgtt ttgatccgta gtttgcggtg caaaataatt catcccaaaa ggaacgccag
     9781 tataaggctg gcagttccca ttagagaaat ttggctgatt cgctgttgca tgtctagtat
     9841 ctatttgacc gatattcatt ttatttctcc tttgttgatt gtcttttgat gaatgaaact
     9901 ggaaatttac tatcggatgg tcgatctttt gtctctaacc aagtgaccaa agcatctccc
     9961 gcaagcttac ctaactgttc aaaatcttgt gcgatggttg tcagtggcgg gtcaacaaga
    10021 gctgctgctt ggatatcatc aaatccgata attgaaaaat cttcagggat atgataacca
    10081 tgctgtctca agagattcat ggtctggata gcgacaacat cgttttcaca aacaaaagca
    10141 gttacttcat acttagtgat ataagaaagc aattcatcta atgtcgcgct tttgtcatcc
    10201 agtgaagtgt ggaaagtcaa accagctttg tccaatgctt caaggtaacc aaggtaacgc
    10261 tgtcgaacgg attgtggatg agtctgcgta ccaaacaaat aagcgatttt tgaatgtccc
    10321 tgttcgatca aataagaggc agcaagcgca ccgccttgga aattatcgga aagaacggta
    10381 ggaaaaggta actcatagat ttttttatct aatatgacga cagggaattc tttcaatgaa
    10441 agctcaaaaa gtaagtcaag atgttgttct gtatcaagag cataataaat caatccgtca
    10501 aattcattga ttagctcttt tgctgtttta ttttgtagaa agtcaagcgt cgtcatcatg
    10561 acatcaatct gtttatcttg catcacaggg ttcaatccct tgctaaaatc accaacagac
    10621 aaatcagcga catatggaag aagaaacaga atccgtcgtg tttttgtagg agaagcaaga
    10681 atctgttccg gagaagcgga tttaacgaaa ctgcctttcc cgcgaacgcg ataaataagt
    10741 cctaattgct ctaattcagt caatgcacgt ttggaagtga ttctgcttac tttataggta
    10801 cgtgaaagtt ctttttcagt tggtagttgg ctgtctacag gtaattcacc agttaaaata
    10861 gccgtttgca aatcattaaa tattgtctgg tacaatggtt gagtcatata aacacctctt
    10921 aatgatatat catattcaaa taataccata atggatgaaa aagtaaataa aaagatattt
    10981 tttgaaagcg tagacataat tggctgtatg aagtataatc tttaaagata tatcaaatag
    11041 aaagaaggtt ttattagtgg tttataaaga tataccaaaa tcggtgcaga aatttatgga
    11101 agatatcaca caaaaatgtg gggatatcca tgcggactgg gcaaagaatt ttacagcagc
    11161 atttgccaat actctgttga caacagtcaa aaagcaaaat gacggaacca cctttttatt
    11221 gacgggagac attccagcga tgtggttaag agactctact gcacaagttc gtccttattt
    11281 agtgattgca aaagaagatg aggaattagc tgacatgatc agcgggttag tcaaaaagca
    11341 atttttttat atcaatatcg atccatatgc gaatgccttc aacgaagaag ctaacggtgc
    11401 tggccaccaa acagatcata caaaaatgaa cgattggatc tgggaaagaa aatatgaaat
    11461 tgattcttta tgctacccaa tacagttggc ctatcttctt tacaaaaata caggaaaaac
    11521 tgatcaattc aatcaatctt ttattgaagg tatcaaaaaa atcttggacg tgttcaaaac
    11581 agagcaagat catgaacact ctccttatac atttgtacga gacacgacaa gaaaagaaga
    11641 tacgctgatc aatgacgggc gaggtgcaaa aacagctttt acaggaatga catggtctgg
    11701 tttccgtcct agcgatgatg cttgccgtta tggatatctg gtaccatcca atatgtttgc
    11761 ggtggtcgtg ttaggttatg tggaagaaat ttttcaaacg atactcgatg ataaagaaat
    11821 tgtacagcaa acacagaaat tgaagaaaga gatccaagag gggattcaga atcacggacg
    11881 aacggttaat caaaaagggc aaacgattta tgcatatgaa gtagatggaa tgggaaacgc
    11941 cacagtcatg gatgacagta atgtgcccaa tctgatcgcc gcaccttact tgggatattg
    12001 ttctgaacaa gaggaagatt atctgcaaac aagaagaaca ttgttgagta aagaaaatcc
    12061 atatttctat gaaggaaagt acgcaaaagg aattggatcc tcccatacgc cagaaaaata
    12121 cgtttggccg attgcattag ctatggaagg aatgacaacg tcagacaaat ctgaaaaaga
    12181 gcggatattg aatttacttg tagcaactga cgcaggaaca caccttatgc acgaaggatt
    12241 cgatgtgaac aatcctgaaa attatacgag agaatggttt tcttgggcaa atatgatgtt
    12301 ttgcgaatta gtcatggatt actttgatat tagagtagaa aagtaggagg tatcgtaaat
    12361 gaaaaagaaa gtatatatca tttctcatag ccactgggat cgtgaatggt acatggctta
    12421 cgaacagcat cacatgcgac tgattgaatt gatagacgat ctactagagc tatttgaagt
    12481 tgatccgtcc ttcaacagtt ttcatttgga tgggcaaacg attattttag atgactatct
    12541 tcaagtaagg ccagaaaaaa gacaggcaat ccaacaagca atcaatgaag gaaaattgag
    12601 aattggacca ttttatatct tgcaagatga ttttcttatc agctcggaat cgaacgtgag
    12661 aaatatgctg attggcatgg aagaaagccg taaatgggga acccctgtca tgttaggcta
    12721 ttttcccgat acatttggaa atatgggaca gacgcctcaa ttaatgaaac aagctggtat
    12781 ttcagcagca gcgtttggcc gtggtgtaaa accaattggc tttgataatc aagttttgga
    12841 agcagaaaac tattcttccc aatactcaga gatgtggtgg aaaggccctg atcaaacagc
    12901 aatctttggt ctgctatttg ccaattggta tagcaacggg aatgagattc cagtagaaaa
    12961 agaagcagct cttgcctttt ggaagcaaaa attagcggat gcagaacaat atgcctctac
    13021 gaaccactta ttgatgatga atggtgttga tcatcaaccg gtacaaaaag atatatcaaa
    13081 agcaatccat ctcgcaaatg aactgtttcc agattacgag tttattcatt ctaattttac
    13141 tgactatctg gaagcagttc aaaaagatgt gccagaagac ttaggctcag tagaaggaga
    13201 attgacaagt caagaaacgg atggctggta tactttagcc aatacagcat ctgctcgggt
    13261 ttacttaaaa caatggaata ccaaggtcca gcgacagcta gaaaatatta cagaaccact
    13321 tgcgacgatg gcttatgaag ttagtggaaa ctatccccat gatcaattag attatgcttg
    13381 gaagacatta atgcaaaatc atcctcacga cagtatttgc ggttgttcag tcgattctgt
    13441 ccatcgggaa atgatacctc gttttgaaaa agcagatgaa gttggaaagt atcttgcaca
    13501 agattcctta gaacagctga ccgcagcaat cgatacgacc ggattcccaa aagacagctt
    13561 tccatttgtc attgtaaata ctgcaggaat ggataaaact ggtgaagcag aaataaccat
    13621 cgaattagaa agaaaaagat tcgctgaagg aatacctgaa caactatatc aagagttaga
    13681 aaatttgcct aaacgtaagt atcatgtaga aacaaaaagt ggggccacta taccagctat
    13741 tttatctgaa gaaactgtcc aatttggtta tgatttgccg aaagaccgtt tccgtgtgcc
    13801 atatatggca agaatgatca aagtgacttt accattagaa aacatgccag cattttcttg
    13861 ggaaacattc gcgcttgtag aaggggaagc gaaagcagaa gaaaaggaaa caatgattca
    13921 tcagagtgga cgaatcatcg aaaacggccc tctacatttg acgattgaaa aaaatggtac
    13981 cattacaatg gaagatcgca aaaacaaacg aaagttgaat aatctgcata tttttgaaga
    14041 tatcggcgat ataggaaatg agtatatctt taaacaacct ttctgtgatc agccaatcct
    14101 atccagtaac aaagaaaact ctgaagtaaa agtattagta gatacaccgg aaatagcaaa
    14161 aataagtatt ctacaggaaa tggaaatccc tgtttctgca gacgagcgtt tagaaaaaga
    14221 acagcagatg gttgtggaat ttcgttatcg aaaagcagaa cgatccaaag aaaaacgaat
    14281 ccttcaaata aaaacaatca tgacaatcag aaaagatagt aaaaaaatcg actttgaaac
    14341 gactctcgac aatcagatga aagatcatcg cttacgtgtc ttgtttccta caaagttaca
    14401 tgtggaacat cacgaagcag atagtatttt tgaagtggtg aagcgcccaa accatgtgtc
    14461 taaaagttgg gaaaacccca cgaatccgca acatcagcaa gcatttgtca atattcatga
    14521 tgaagagtac ggtgtaacag tcggaaactt tggtttgaat gaatatgaag taacagaaga
    14581 cggacaaatc gcagtgacgc tacttcgcag tgtgggagaa ctaggcgatt ggggatattt
    14641 tccaacacca gaagcacagt gtctaggtga gcatcggttc aattacagca ttgaactaca
    14701 tggaccagaa gaaaaattct ctacttattt gcatgcatac gcagcacaga ttcctttttc
    14761 cactcaacaa atcaaacatc atgaaggaac attgatttca aaacaacaat atttaacgat
    14821 aaaaagtgaa acgtttgcaa ttacagcttt gaaacgcagc aagtttagtg ataaagtagt
    14881 agtaagaggt ttcaatatgt ctagtcattt agaaaaattg gaaataacta aagacaatgg
    14941 aaaaactgtc atattgaatc tgcttgaaga accaacgaag caagcggttg tcccaataat
    15001 ccaaccttat gagattcgga caatcggatt cgaggaggaa aacgaatgta tggcggaata
    15061 gaagcaggcg gaacaaaatt tgtgtgtgcg gtttcagatg gtggagaaat tttagaaaaa
    15121 ttaagtatcc ctaccacaac accagaagaa acattggatc aggtatttga attttttgat
    15181 cgttatgaat tgaagggaat cgggattggc tcattcggcc cgattggtat cgacaaaaca
    15241 aaagataatt acggatacgt tttggcaact cccaaaaaag ggtgggcaca attcgatttt
    15301 cttggcacaa tcaaggaacg ctacaatatt cctgctgctt ggacaacgga tgtcaatgct
    15361 gctgcatatg gggaattgaa gcaaggagca gcacaaggaa aaaatagttg tatttacctt
    15421 accgtgggaa caggaatcgg tgcaggtgtc gtgatcaatg aagaaatttt ttcagggatc
    15481 gcacatcctg aaatgggaca tatttgggtg aaacgccatc cagatgacca ttatgaaggt
    15541 acttgtcctt atcataaaga ttgtttagaa ggattagcag caggaccgtc tattgaggca
    15601 cgcacaggga tcaaagggca agatctgccg caagatcatc ctgtttgggc tattcaggct
    15661 tattatattg ctcaagcact gatcaactat acgttgacgt tagcacctga aaagattatt
    15721 ttaggcggtg gggtaatgaa tcaggatcat ctccttcaaa aaatccgtca gcaatttgtt
    15781 gaattgatgg gtggttatat ggaaacacct caagtaagtg aatatatcgt tcgttgggga
    15841 ctgccgaatg aaagtgggat tatcggtagt ttgctgttag cagaacacga acatcaaaag
    15901 taagcagaca aattacacaa aaagaacaga gtcttatcat tatctgctga taagactctg
    15961 ttctttttta tttctattcc cataatccag gtaaagcttt accgactatt ttttcttgag
    16021 taaatggatg gatcatgtgc agttctaatg catggagcat cagtcgttca gctgttctat
    16081 tttggtagag agggtctcca acgatcggat gtcccttatg agctaaatga actctgatct
    16141 ggtgtgtacg tccggtttcc aaaacacaat agacttgtgt ttgttcagaa agtactttgt
    16201 tgacagtgac atgtgtcaag gcgctctgac catttttatc atctacgacc ctttttctcc
    16261 gatcatggcg atctcgtcca attttattgg tgatggtcat atctttggca atttctcccc
    16321 atacgatggc ttgatatctc cgatagatca ttttttgttc caacattctt ccaagtatcg
    16381 gcaagacaaa tggatttttt gcaaataaaa cggcaccgct tgtttctttg tccaagcggt
    16441 ggacaacgta aggtcgttgc tgtttagtgt cgagataagc agctaaatga ttgagtaagg
    16501 tactgttttc tgtcggttca tttggatgag tcttcattcc tgcaggctta tccactataa
    16561 tcaaatgctc atcttcatat aaaacacgga tgtgcttcgc atctcctaat tgaacttctt
    16621 gataattata atctgtttct tcgaataaca atgtgatctg gtcattgccc tttacttcat
    16681 gatggaacaa agcaggttgt tgattgatcc aaacgttttt acgtatacgc aaaaagtgtc
    16741 gaacctttct aggaactaac cattcctttt caagcaagtc gcgaattgtt gtttcaggat
    16801 ggttcatagg taaagtgatc gtgattttca caagtgtact cctttcagtg caattgcaga
    16861 ctaattgtac catttttaaa acatatttaa gaaaaaactt acatttttct gttcattctt
    16921 atgttactct acttgcacta acgtaaaaaa acatattgaa ctacaactta agcaggaaga
    16981 aaacactctt tcactatggt aaaataagac gattgaatca aatagacagg agaagaagca
    17041 aatggatttt caaagcattt tagaaaagat aaaagctgct ttgatcagtt tttggcgttg
    17101 gatcagacca tatctcggac aattccatcg atggcgaaag cgcatctgga aaaaatatca
    17161 tgtgaacaaa ttgattttgc tgattggttt gatctgtgtc ttagtgacaa gtatctacct
    17221 ctttatttta gcaaaacaag caaatgtcga aacgttaaaa tcaggattaa gccaatcgac
    17281 agtcatttat gataagaaaa acgaagaagc tggcacactg tacgcccaaa aaggaacata
    17341 tgtagaatta aacgcgattt ctccctatat ccaagatgca gtaatctcga cagaagatag
    17401 gaatttttac gagcatcacg ggtttgatat caaagggatt gcccgtgcag cagtccgaat
    17461 ggtcttgtct ggcggaacat cagggggcgg aggaagtacg atcactcagc agcttgccaa
    17521 aaatgcgtat ctgacattag accaaacgtt tgatcgaaaa gcgaaggaat tatttttggc
    17581 aatcgagatc gaaaagaagt acagcaaaga agaaatcttg accatgtatc ttaacaatgc
    17641 ctattttggc aacggggtat ggggtgtcca agacgcttcc agaaagtatt ttggagtaga
    17701 tgcaacgaat gttacgctgt ctgaggctgc tactttggca ggaatgctga aaggtcctgg
    17761 tatttataat ccgatcgatc acccaaaaaa tgcgaatgat cggaaaaaca cagtactaag
    17821 tgtaatggaa gaaaacggaa aaatcacaaa agaacaagaa caagcagaaa gccagacaga
    17881 tatcagcgcg tatcttaatg acacatacga taattcagat tctggttatc gttatcctta
    17941 ttattttgat gcagtgatcg atgaagcagt taacgagtat ggtcttgatg aagaagatgt
    18001 catgaataaa ggctataaaa tctatacagc tttagatcag gattatcaaa agcaattaga
    18061 agctacttat caaaacagtg cattattccc agcaaatgct tcagatggtg ccatggtcca
    18121 atctgcttca gtagctttag atccgaatac aggtggtgta caagcactag taggacgaag
    18181 aggagatcat gtctttagag gattcagctt tgctacacag atgcgtcgtt caccaggatc
    18241 aacgatcaaa ccattaagcg tatatacacc agcactggaa gctggataca agccttccag
    18301 tatcttgaaa gacgaaccgc aaagttatta cgatgcgcat aattttgacg ggacatacca
    18361 aggagaagta ccgatgtatc aagctgttgc acaaagtttg aatttgcctg ctgtatggct
    18421 tttgcatgag atcggtttac aaaaaggata tgataaagca gaagaattcg gcctgccttt
    18481 agcaaaatct gataagtatt atggcttagc ccttggtgga ctggaaaaag gagtatctcc
    18541 attgatcatg gcgggtgctt acagtgcatt tgccaatgat ggtcagatgt atacgccgca
    18601 tcttatcacg aaaatcgttg attcgactgg agcggtgatc gtcgataaca caaacaaaaa
    18661 accaaaacag gtcatatcaa aggaaacagc cgatgaaatg accagcatgc ttcttggtac
    18721 attttcaaat ggaacagcgg ctgctgcgaa tcctgcaggt tatacgatag cagggaaaac
    18781 aggaaccaca gaaactaatt tcgatgccac aaaagcaaat gaccaatgga tgatcggtta
    18841 cacgccagac gtagtgattt cgacatggat gggttacgaa gaatccagca agctgcacta
    18901 tttggaaggg acaagtggaa atgttgtcgg gaaagtcttc aaatcagcgg cagaaggcat
    18961 ccttccgtat acttcaaaaa caggatttag tgtagcagat gcctatgcca caggaggaca
    19021 agtggtagct gcagatcaag ttggaaacac aggaaattct aatggagagt ctggaaattg
    19081 gcaagataac ttgaatcaat atggtgaaca agcaaaagaa gggctgaaaa acttcggcga
    19141 tatggtgaaa gaaggcgttc agggaattgg tgaagcggct aaagacctct ggcggaaata
    19201 tcaaggagag tagccccttt gaaaacgaat aaccccatgt tacaatagag agtaactact
    19261 aaaagaaaag agggcataac atgtcaaaca tctacgatag cgcgaatcaa atcgaacgtg
    19321 agatccgtga attaccagaa ttcaaagcgt tgaaagaagc gtacgagaaa gtacaagcaa
    19381 acgaagaagc acacaaactg ttcaaagact tccaagcgat gcaaatcgaa ttacaagaaa
    19441 aacaaatgag tggacaagag ttcagcgacg aagatgcaat gaaagcacaa gaaatggctg
    19501 tgaaaatcca atcagaagaa gtaatcaatg acttgatgca aaaagagcaa ggcttcagta
    19561 caatcatcaa tgacttgaac cgtatcatca tgacaccagt tcgcgacctt tacagcgaat
    19621 aaaactattt tatgaaacaa cagagggtgt gaaagagacg ttcggccttg agcgatgaca
    19681 aaattgcttc ttattgccga taggctgtct cttgaacacc atttaccaag aggtcgtgag
    19741 cctaccgttt aactctgagt tacaaggaac aatttcaaaa aacagcttct catgtttgat
    19801 gactattgtg acttgtgacc gaggagttgg caggagaaca ccgtttattc agaaatagga
    19861 gctacgacaa aagtcttgtc atagctccta ttttttgtca ttcatttttc aagcggtaga
    19921 aatagccttt ttgagctaac agtttttcgt aagtacctat ttcaacgatt tttccttcat
    19981 cgatcacaac gattttatct gcctctttga tcgttgaaag ccgatgagcc acaataaaag
    20041 tagtgggtgt ttctgtcagg taattcaggg cttgctggat ttttttctct gactggttat
    20101 ctaaagctga ggttgcttca tctagcaaaa tgatttttgg ctgacgtatc aacgctcgtg
    20161 caatggagat acattgcttt tggccaccag acagcttgtt gccatgttcg cctactaagg
    20221 tatccagacc atcaggaagc tggtcaatca gtgaagaaag ttgcgcagct tcgatgactt
    20281 catctaaacg ttcttttgat acgtttttca agccataagt gatgttctct ttgatcgaag
    20341 cggagaacaa caaagtggtt tgaggaacaa cggctaaata atttcgcaca cttcgcatat
    20401 ccagttcatc caaaggctga tcatctagaa aaatttttcc tgaagtcggc tggataaatc
    20461 caatcaataa gttaaggagt gtagattttc cagagccaga tggcccaaca aaagcaattg
    20521 tttctccagc tttcacctct agagaaaaat gatcgataat cggtttgagt gagtccggat
    20581 aaagaaaaga aacatcctca aaacgaaaat ttccatccaa atgttggatt tttttcttac
    20641 cgtgataagt ttctacatcg ccagcaatca acagttcgcc aatagaagtg atagattcca
    20701 tgccttttga aatcgtagga atcagcgtga tcattccaga aacggaattg acgatcgtcg
    20761 tgaaatacgt ctgatacatc acgacatcgc cgggttggat gcgcccttgt aaagctaata
    20821 tcccagtgaa actcaaacaa atcagctgaa atatctgaaa actagcccag ctgactgatc
    20881 cgaaaacaga ttgcaggata tctaagtgga atcctttttc atagatttga gtaaaatgct
    20941 tatttaagcg agttgtttcg tgtttttcta acccatgagc tcgagcaatc ggtgctaatt
    21001 cgatcacttc gatcactttt gcagaagtct cttccatttc ttgcctgaag tctgagttgg
    21061 actgtctgat ccttttacgg aaaaagagag taacgaatgc ggcgagagga acagtcaata
    21121 agaaaaacat gaaaacaaca ggacttttat aaagcgtgat acctaaagct acgaataaat
    21181 tgacagcgat atttaatccg ctgacaaata cttgagagga caacgtttgg atagcttcta
    21241 catcacggat gatctttgac tggattcgtc cagattgcaa ttctttttga taaggaatag
    21301 atagttcttg gattttcttg ataagggtcg ctcttagtcc agcttctact tgacggatcg
    21361 aagtgctatg gtattttaca tgaaaataat tcgtcaatat attttgacca atcagacaag
    21421 ccatcaaaaa agcgttgaaa taaatggcct gcaactgaga agaatctttc gatgaagctg
    21481 cattgattac attggcaata gcgatcggca ttaaccaaga tggaagatgt ttgataataa
    21541 aaaagagtcc tgacatggca aaacgccaat aatttccctg ataaagatgc agcaatgtct
    21601 gaaaagcatt tgcatgatat ttttgataac ttttctgtat tcgttcctcc ataatcttct
    21661 ccttaacaat cttggtaaat aaactgcaag tgttctattg cttcaggaac ataaatctca
    21721 ggagtagctt caagagtggc gtacattaga ggacgctcat atttgatata atgcagtacc
    21781 gggcggaaat ctaaacagcc cttgccaaca ggtaccatgc ggatctttcc attttccatg
    21841 atatagtctt tgagatgaag cgctgtaata taaccatgta aattgtccaa ggcacctcgg
    21901 atcacttttt cctgatcagc atgattttct ttatgcatca aattcgcaca atctaaaatg
    21961 atttttaaat ttggtgaagc tacttcatcg attaaacgtt tagctaactg atagttatag
    22021 attggatgat tgatccctgc ttcaacagca acagttacgc caaaattttc tgcatgagcg
    22081 actaattcaa tgatagaatt tctggcgatc tgataagctt cttcagtaaa attttctttt
    22141 gtgtaaccga cagagacact accggtttcc gtagcaatca tcttggcttg ataggcagag
    22201 cacagagcaa tttgctgttt gaaagttgcc aatgcttgtg cacgaacttc ttgatttttg
    22261 ctggataggt tgatatagca tcctaaaatg gaaagttcga tattgttttt ttggaagtat
    22321 tcaccaaaat aggcagcact cccaagtgtc atgtcagtta atttaagttg ttcaggaaat
    22381 gctttttgtg gtgcaaattg gatatgggac aagtgaagct gtgatacttt ttgaacgatt
    22441 tcttcacgat ttcgaacatc taaatcgtgg gcacggatgc ctaaatttaa tggaaacatc
    22501 tcaaactcct ttttgtaaat aattagcaag aggcagacgg gattctttta tcaattcaac
    22561 aattattcgg gcaactttat cagcgccttc tggactaaag tgggtattgt cttttagtcc
    22621 ttctgggtaa tttttacaag tatcaggcat aagatgcagg tagaattttt ttgtctcttc
    22681 ttttgagaag gtctggaaca attcttgtga tcttgagaac acatctaaaa gaggaatatg
    22741 gtgtttctca gcaaatgcac gcatcgcttt tggatagtct ccaagtatat ctggattcaa
    22801 tgttccgttt tctaagtaat ctcgtcgcgt cacagatgtc agtaagacca tttggacgtt
    22861 cgcttttttt gcgcattcaa aaaatatctg cagatttttt aaatagctac cgaagggaag
    22921 cgtacctctt tcttcttgga ttttctggtc gttatgtcca aattgtacaa ataagaaatc
    22981 attcggctga aactgtgaag ctagacgggc taaccttcct tcctggataa atgatttggt
    23041 gctgcgtcca ttctttgctt ggttattgat ctggatctcg ggtgaaaaat aagcagagat
    23101 tttttctccc caacctgtct cgggacgctt gttttcatct ttttctgccg cagtagaatc
    23161 accggcaata tggatagtag gcatgttatc tcttctttca taaaggttta taggcaatga
    23221 agcagaacag caagagaaga agacggagca a
//
LOCUS       DAZBWR010000037        23089 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000037 DAZBWR010000000
VERSION     DAZBWR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.037"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..2686
                     /locus_tag="ACHGT5_001274"
     CDS             215..2686
                     /locus_tag="ACHGT5_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN7217270.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2789..4525)
                     /locus_tag="ACHGT5_001275"
     CDS             complement(2789..4525)
                     /locus_tag="ACHGT5_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7217271.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4522..5226)
                     /locus_tag="ACHGT5_001276"
     CDS             complement(4522..5226)
                     /locus_tag="ACHGT5_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7217272.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5357..5860
                     /locus_tag="ACHGT5_001277"
     CDS             5357..5860
                     /locus_tag="ACHGT5_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN7217273.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5820..6158
                     /locus_tag="ACHGT5_001278"
     CDS             5820..6158
                     /locus_tag="ACHGT5_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN7217274.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6179..7597
                     /gene="ffh"
                     /locus_tag="ACHGT5_001279"
     CDS             6179..7597
                     /gene="ffh"
                     /locus_tag="ACHGT5_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN7217275.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7709..8287
                     /locus_tag="ACHGT5_001280"
     CDS             7709..8287
                     /locus_tag="ACHGT5_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN7217276.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8291..8812
                     /locus_tag="ACHGT5_001281"
     CDS             8291..8812
                     /locus_tag="ACHGT5_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN7217277.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8891..9445)
                     /locus_tag="ACHGT5_001282"
     CDS             complement(8891..9445)
                     /locus_tag="ACHGT5_001282"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN7217278.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9698..9973
                     /gene="rpsP"
                     /locus_tag="ACHGT5_001283"
     CDS             9698..9973
                     /gene="rpsP"
                     /locus_tag="ACHGT5_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN7217279.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9985..10236
                     /locus_tag="ACHGT5_001284"
     CDS             9985..10236
                     /locus_tag="ACHGT5_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN7217280.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10361..11152
                     /locus_tag="ACHGT5_001285"
     CDS             10361..11152
                     /locus_tag="ACHGT5_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217281.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11322..11843
                     /gene="rimM"
                     /locus_tag="ACHGT5_001286"
     CDS             11322..11843
                     /gene="rimM"
                     /locus_tag="ACHGT5_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN7217282.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11833..12594
                     /gene="trmD"
                     /locus_tag="ACHGT5_001287"
     CDS             11833..12594
                     /gene="trmD"
                     /locus_tag="ACHGT5_001287"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN7217283.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12730..13077
                     /gene="rplS"
                     /locus_tag="ACHGT5_001288"
     CDS             12730..13077
                     /gene="rplS"
                     /locus_tag="ACHGT5_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN7217284.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13450..14112
                     /locus_tag="ACHGT5_001289"
     CDS             13450..14112
                     /locus_tag="ACHGT5_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217285.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14191..17418
                     /gene="fss3"
                     /locus_tag="ACHGT5_001290"
     CDS             14191..17418
                     /gene="fss3"
                     /locus_tag="ACHGT5_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN7217286.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17532..17846
                     /locus_tag="ACHGT5_001291"
     CDS             17532..17846
                     /locus_tag="ACHGT5_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN7217287.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17859..18233
                     /locus_tag="ACHGT5_001292"
     CDS             17859..18233
                     /locus_tag="ACHGT5_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN7217288.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18243..18587
                     /locus_tag="ACHGT5_001293"
     CDS             18243..18587
                     /locus_tag="ACHGT5_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN7217289.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18658..20010
                     /locus_tag="ACHGT5_001294"
     CDS             18658..20010
                     /locus_tag="ACHGT5_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN7217290.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20070..21212
                     /locus_tag="ACHGT5_001295"
     CDS             20070..21212
                     /locus_tag="ACHGT5_001295"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFN7217291.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21237..21491
                     /locus_tag="ACHGT5_001296"
     CDS             21237..21491
                     /locus_tag="ACHGT5_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217292.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21747..22931
                     /gene="mobT"
                     /locus_tag="ACHGT5_001297"
     CDS             21747..22931
                     /gene="mobT"
                     /locus_tag="ACHGT5_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN7217293.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22928..23065
                     /locus_tag="ACHGT5_001298"
     CDS             22928..23065
                     /locus_tag="ACHGT5_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN7217294.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7675 a         4105 c         4967 g         6342 t
ORIGIN      
        1 ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg attttccacc
       61 aacacaaaat attatagaac cctttttttt tgctatatga caggaatgaa aggtgtttgc
      121 aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag aagtttggaa
      181 atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga taaatcgaag
      241 taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt ttccatcgta
      301 taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa
      361 ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca tatttacgta
      421 tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat cctataagaa
      481 cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc aggaaattcg
      541 atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca atgtcatacg
      601 agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca ttgttatttt
      661 agagactgga tatcaaattc cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa
      721 tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggtg atccattaat
      781 gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag ttggagcaga
      841 ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag aacagtcgga
      901 gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta ttcctatcgg
      961 tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag aaagtgtcgt
     1021 tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc tgacgacgat
     1081 tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac acaatatgaa
     1141 aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag gaacgatcac
     1201 agaaaataaa atgtctgttc aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca
     1261 gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag gagacaatgc
     1321 aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct atcaccatgt
     1381 tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt
     1441 tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt
     1501 ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga
     1561 agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg ctaatcctat
     1621 cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag ccatcaaagt
     1681 gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag ggatttcaca
     1741 tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag cagctgtcga
     1801 aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat
     1861 aaagcgtaac aatacagtag ctatgacagg tgatggcgtg aatgatattc tagccatgaa
     1921 agaagcagac tgcagtatag cgatggcttc cggaaatgaa gccgccatgc aggcctcaca
     1981 agtggtctta cttgattctg acttttcacg catgccagaa gttgttgcag aaggacggcg
     2041 cgttgtgaac aatattgagc gatcagccag cttgttttta gtgaagaata ttttctcctt
     2101 tttgctctca ctgttttcag tgatctttgc tctgacctat cctttagaac catcacaaat
     2161 cacattgatt agtttgttta ctatcggact tccatctttc ttgcttgctc tagaagaaaa
     2221 caaaaagcga atcagaggga aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg
     2281 actaacagat atgatcgtag tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa
     2341 taaaacagat gtttctacag catctaccat gcttttgatt gcagtagggt tcctcgttct
     2401 ctataaaatc tgttcaccgc tgaataaatt cagaagtcag atcattcttt tctgtgctag
     2461 cggtaacttt ttttcggtca tctttttgca taagctgttt tcaatcaccg gtatctcagc
     2521 tgtatcactt ttgctgttga gcattctatt tttctcggca gattcgattt ttaggcattt
     2581 gaccacattg gtagaatatt tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag
     2641 tcgcgtagct aaaaaatttc tatcgatatt taaacgagaa aattgacgaa aagtatctca
     2701 aaaaagagta aaaaagaaca accactcgtg tatctttcga agcaggatga gtcatccaac
     2761 tttaaaacgc acgagcagtt gctctttttt acattttcgg aatggtaacg gtgaaagtcg
     2821 aacctgttcc aagatgactg tctacagtaa tcgtaccatt aaggagttgc acgtagtctt
     2881 ttacaatcgc taaccctaat ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc
     2941 ggtaaaaacg ctcaaagatc cgttcctgat cctcaatgtc gatacctata ccaaagtctt
     3001 ggacagaaaa cgatagatca tctgttgcct gatagcggat aataatctcg ctgtcgcttt
     3061 ttgaatattg gacagcattt tcaattagat ttttgataat tggataaagg atatctgttt
     3121 tagtagtaaa tggttcgttc tttccaatca atcgaatcgt caaccgtttc ttttcgatga
     3181 ttggctgata attttgaacg atttgctgga aataaggttc tgtataaatt gtctggatct
     3241 cgtaagaatt ctcgccatct tttgacagct ggatgatttc ccgaatcagt ttatctaacc
     3301 ggatcgcatc tttttgcatg atctcaagaa aagaagtcag agtttgcggg tcatcttttg
     3361 caccatccag taaagtctcg gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt
     3421 gagagacgtt gccaacgaaa tccttttgta gcttttctaa acgtcgcact ttcgttaagt
     3481 catacgccac ccctagaatc tgtccggtac cttctgaatt agtaaagtag cgcaaactca
     3541 agtctaatgt ctgccgacct tctgtaaggg tgatctcctg atggatcagc ggatgctcag
     3601 gagtcacttg atgaatcagc tgaatgagtt ttggctcttg aatgacttct gtgtaccgct
     3661 gttcaggttg atagcttacc acgtttaaat gtagctgcat ctttggatta agcagtagta
     3721 aacgagaatc agcatcatca atcaggaata cgccaatcat cagttcgttc agtaacgtat
     3781 ataattgctc ttctgtggta gtacacgccc gatacatctt actcatttgt tcgctcaacg
     3841 cattaatcgt ctgatacaat tcttcccatt gatcgggtgt ttgcatgatt acttccgcct
     3901 gattaggatt ttgcaccatt ttttttagta cgggcagcac ggttttcaat ggctggtttt
     3961 tttgatagat cagataatag atcattcctg caagaacaag gaagaaagac agaaagaaga
     4021 aaaagaccca acggcgaaaa ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga
     4081 tacgtaaaat ggcttctaac tggccacttt ttttgactgg taatgcgaca tataaaagat
     4141 cctcattcaa agtagtactt ttgcgcaaag cgctacctag gttcccacca ttcaatacgg
     4201 ctttgatttc gggacgggag tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata
     4261 agatctttcc agtagcatcc attaaagtaa tccgttcgtt cgactgatgg acgaattctt
     4321 ccaaagcagt tttgttttgc gctgcttgga ggttttcgac atctaattgc cggatcagca
     4381 gttgtgcttt ttttgtcagg tagctttctt gctgttccac gatctgctgt tgaaaataat
     4441 gggaaatcaa ttgccagcca ccaaaaaaca aactaactaa aagaataaga aagaagagac
     4501 gttcataaat ttttttccat ttcatcgttt gggttcctga aacttatagc caaatcctct
     4561 aatagtcacc aagtactttg ggtgttttgg atctcgctcg attttttctc gcaaatgact
     4621 aacgtgtaca tctacgattc ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag
     4681 caatgtgtct cggtcgatca cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa
     4741 ttctttcggt gttagttcaa tcagatggtc gtctacggtc acttgataat tggtcaaatc
     4801 agcagtgatt tgtccaatcg atagatattc aggctcagct tcatcttttg attgcttacg
     4861 aggttcaatt cgtctgaata ttgctttcat acgggctaat acttcgcgag gactaaatgg
     4921 tttggtcaaa tagtcgtcag cacctatttc taggccaatg atccgatcca cttggtcatc
     4981 tttagccgtc agcatcaaaa tcggtgtttc atttttttct tgacgcagcc gctgagtgat
     5041 ctccatgcca tccatcgttg gaagcatcac atctaaaatg atgaaatcaa atgattgttc
     5101 taatgctaat tctaatcctt cgcctccatc tgtggcactg gttaccttgt agccttcttt
     5161 ttccaaattg aacgtcaaga gcgttacaat agaaggttca tcatcaacaa ctagtatttt
     5221 tttcattcaa atctccttta aataggtttc attctgttta tattttagca tagaatcagt
     5281 acaaagggct attttacaaa agaaataaat ggtataatag gaaggcactt tgctgaatag
     5341 aaaggaagag cagtcaatga ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta
     5401 tgatggacag aaaaaagaag taccagaatt gatggaacta gttgaaactg gagaagctgt
     5461 catggtctgt ccagaagtga tcggcggatt accaattcct agagatcccg ctgaaatcat
     5521 tggtggaaat ggattcgatg tgtggaaaga tcaagcaaaa gtatggacga caagcggcga
     5581 agatgtaacg gaagcgtaca aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa
     5641 acacattgat ctagtcatca tgaaggaaaa aagcccttca tgcggaaccc attcgattta
     5701 tgacggaagt ttttccggaa taaaaaaaaa tggtgccgga gtggctactg cttattttat
     5761 tcagcaaaaa atgactgtgc tctctgatga agaatggctg aaaactagag gtgagcagaa
     5821 tggaaatcga aaagaccaat cgaatgaacg cgctctttga attttattcg accttattga
     5881 cagaaaaaca aatgaactac atggaattat attatgcaga tgatttttca ttaggtgaga
     5941 tcgcggaaga atacgaagta agccgacaag cagtatatga caatatcaag cggacaagca
     6001 agatcttgga ggattatgaa aaaaaactcc atcttttttc taattatatt gttcgagaac
     6061 aggtattgga aaatatgact gcatatatta cagataagta cccggaagat aaaaaactac
     6121 ttgaatatgt acagcaaatt caagcaatcg aagaataaat aaaaggaatg aagaaaatat
     6181 ggcatttgaa agcttaactg aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg
     6241 aaaagtatca gaagctgacg taaaagaaat gatgcgggaa atccgccttg ccttactaga
     6301 agccgatgtt aacttgcaag tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt
     6361 aggggcagaa gtgctggaaa gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga
     6421 agaattgacg atcacacttg gttctgaaac agcagagtta aataaatcac caaaaatacc
     6481 aactgttatc atgatggctg gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt
     6541 agccaattat ttaaagaaaa atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata
     6601 tcgaccagct gcgatcgacc aattgaaagt attaggtcaa cagctagacg ttcctgtatt
     6661 tgatatggga actgaagtca gcccggtaga gatcgtccgt caagggatgg agttagcaaa
     6721 agaaaagaaa aacgactatg tattgatcga tacggcagga cgtctgcata tcgatgaaac
     6781 attgatggat gagttgaaac aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt
     6841 tgtcgatgcc atgactggtc aagatgccgt caacgtagct gatagcttca accagcaatt
     6901 aggtattaca ggggtcgtca tcactaaact tgatggggat actcgaggcg gggcagcact
     6961 atcgatccgc tcagtgacag gcgcaccaat caaattcatc ggttctggtg aaaaattgac
     7021 tgacttagaa gtattccacc cagatcgtat ggctagccgt atccttggta tgggggatat
     7081 gctgacattg attgaaaaag cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc
     7141 acagaaaatg cgggaaaatt cattcgattt caatgacttt attgaacagc ttgatcaagt
     7201 gatgggtatg ggcccactgg aagatctaat caaaatgatc ccaggaatga atcaagtccc
     7261 tggaatcgaa aacgtcaagg tagatccaaa agatgttgaa cgcaaaaaag cgatggttta
     7321 ttctatgacg ccagcagaaa gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg
     7381 aattgctgcc ggttctggaa acagtgttgt tgaagtgaat cgaatgatca aacaattcaa
     7441 ggaatcaaga aaaatgatgc agcaaatgtc caaaggggat atgaacatcc caggtatgga
     7501 ccaaatgttt ggttcaggcg tcaaaggaaa actaggcaaa atggcgatga atcgaatgat
     7561 caagaaaaat aagaagaaaa agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac
     7621 agactgaaaa tgtctactgc cggagctttt ctatcagcaa tttttattct ttactaatac
     7681 aattactcta aacgaagagg tggcacagat gagatgtccg tggagtgaac aaacagaaag
     7741 tatgcggcaa tatcatgata ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt
     7801 ccgaaaattg atgcttgata ttaatcaagc cggattgagc tggcagacga ttttaaataa
     7861 aagtgaagca ttcgatgaag catatgatcg attcgatatc aaaaaagtcg caaattatgg
     7921 agaaacaaaa atcagtgaac taatggaaaa caaaggaatt attcgtaacc gaagaaaaat
     7981 cgaagcagcc gtccaaaacg ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt
     8041 ttcagagtat ctttggtcgt tttctaatgg aaaaatcgtt gattcttctt atcattcaca
     8101 agaagaagtc ccaacaacaa gtcctctttc agatgaatta gcaaaagatc taaagaaaag
     8161 aggatttaaa tttatcggaa gcacaacaat ctatgctttt ttagaagctg tcgggatcat
     8221 caatgatcac cttgacagtt gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg
     8281 cgaataaagc atgcgaaaaa tcatatttta tggtgctata agtttagatg gctatttggc
     8341 aacgaaaacg gatgatcttc aatggctttt tgacactcca acaggtaagc aaactactta
     8401 tgaagctttt tatgaaacaa tcgatacgac catcatgggc agaaaaactt atcaggaagc
     8461 aaaaaaatat ttggagaccg aagcaattta tccagagaaa aagaatttcg ttttttccac
     8521 ccacgcacaa ttacaattgc cagatgctga ggtcgtaaac gaagatcctg ttgcttttct
     8581 aaaaaatcta caaatgacag aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat
     8641 caaaccgcta ttggaacatc aaatgattga tgaatggtat atccagatta cccctgtatt
     8701 attaggggac gggattcctt tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct
     8761 agacacgaca cgattcggtg aatttatcga attgcattat gtacgaaagt agaagtaaat
     8821 aaaataaaaa aggcaactat atgcagaaat gtcctgtaca tagttgcctt ttttattacc
     8881 gttatgcttt ttactctgtt aatttgttag aaaaatctag atacgcatct aaaatcgttt
     8941 ggaagaaact taatgttcct tcaacgatat taccattttc atcaattaga ttcgcaacgt
     9001 ttccgatata tgcttccggt tgctgcaagg ttggcacatt caaaaagaca agtgattggc
     9061 gtagatggtg gtttgcgcca aagccgccga tcccacctgg tgatgcagta acgactaaac
     9121 ctggtttatg atcccaaaca ctgtgtccat aaggtcttga gcctacatct aaagcatttt
     9181 tcaatactgc tggcacagaa cggttgtatt caggagtaac gaaaacgaca ccgtctagtc
     9241 ccctcacttc ctcacgaaaa cgagtccatt cagcaggagc ctgttctgga gtttcgagat
     9301 cctcattgta aaatggcaga tcatcgattt ttacaaatac actttcgtac ccttcaggaa
     9361 gcaatttcgc aaaagcttct gctacttttt tgttgtaaga atcttttctt aaactaccaa
     9421 taaaaaatcc aatttttttt gtcatacgat ttttcctcct tcaaactatc tataataatc
     9481 ttacaaaaga taagtaactg gagcaaataa aaacacttac tgacaaaagc tacatagaag
     9541 atagattact tatttttaac aaaaaagaag tctaaaatct tctattttct aaaaaaaatc
     9601 gaatttaatc aagctgtaaa gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta
     9661 catcttgtga ataaaacaat ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa
     9721 acgtatgggt tctaaaaaga gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc
     9781 tcgtgatgga cgtttcatcg aaactgtagg tacttacaat cctttgaaag accctgcaga
     9841 agtagtttta aaagaagact tagttcttga ctggttatca aaaggtgcac aaccttctga
     9901 cactgtacgt aacatccttt caaaagaagg cgttatgcaa aaacaccacg aagctaaatt
     9961 ctcaaagaaa taaggtgacc ggatatgaaa gatttaagcg acttagtctt aacaatcgtt
    10021 cgtccgctag tcacttatcc tgatcaagtc aagttagacg tcgtagagtc gaaagacttt
    10081 tacgagtaca atctaacagt ggctcctgaa gatattggcc gtatcattgg aaaacaaggg
    10141 cgtgttgcta aagcaatccg cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa
    10201 gtgcgtttga atatcatcga tgataaggaa aagtaaataa acgtgctgta ctcgaatttt
    10261 cgagtgcagc ttttttgttt tttttattaa ttgaagaaat gattatttgg caaaacaagc
    10321 aagaaagcgt atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt
    10381 tattccattt gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac
    10441 acatctatca agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac
    10501 ggcttccacc actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga
    10561 ccagttgaaa gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg
    10621 ctatctcaat atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac
    10681 agatcgtccc ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta
    10741 tctatatcaa tatgggtttg cttctagtca agaaacaatc caagttttgc agccatttga
    10801 gatcgacaca aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg
    10861 tgcagcaggt tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg
    10921 gaataatata gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga
    10981 agaaaacagt ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac
    11041 gacgaatcga gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca
    11101 tcaagatcct atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg
    11161 cgctgatctg agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc
    11221 gcatttttgc ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac
    11281 atgctaaaat aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg
    11341 taggaaaaat cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg
    11401 atttcccaga agaacgttac aaaaaaggaa cggtgctaac actattccaa gacggaaaag
    11461 cacctgtgga attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt
    11521 ttgaaggaca tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt
    11581 caaaagacaa ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc
    11641 tgcatgtatt agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg
    11701 ccaatgatgt ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg
    11761 cttctgtcgt caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag
    11821 gactgattga cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct
    11881 atgggggaat ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct
    11941 aacttccgcg attattcaga taacaaacat cagacagtag atgattatcc atacggcggt
    12001 ggagcaggta tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa
    12061 gctgcaccgg atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat
    12121 cagaaaatgg cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat
    12181 gagggatatg atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat
    12241 gtccttactg ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg
    12301 ccagatgtgt tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg
    12361 gaacatcccc aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg
    12421 acaaatggaa atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc
    12481 tatcaacgtc ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt
    12541 ttagaagaaa tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt
    12601 ctaaaatctt ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc
    12661 acttaactat tacgatattc cgctgtgaga aaagctcata agaatatttg gaataaggag
    12721 aagaaaacca tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc
    12781 ccagcgttcc gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt
    12841 gaacgtatcc agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa
    12901 acttatacag tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat
    12961 acaccacgtg ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg
    13021 tactacctac gtgcattaca cggaaaagct gctcgtatca aagaaatcag aagataaaca
    13081 cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt
    13141 cttttttctc tatcacagtt ttaagccatt tttggctgtt ttggaccatt tttcaatcat
    13201 attcccttaa aaattgtcct agattgtccg acattgacta tcagtgtcgt caaaataggg
    13261 caaaatttag tagtcagctt gacgacaaat ataatacgaa aaataccaaa acatttaccg
    13321 aatttagcaa cttttgaacc caagtttctc aaagggtcag ctattagcaa aaaagacaaa
    13381 acatgtactt ttctactaaa aagttgatgt gataggcttc tatctaaata actattagga
    13441 gtttttatcg tgaagcaaaa tgatggacgt atcatttgga aaaatcaaag tgagttaaaa
    13501 ctgattttaa caattaatga attcatcgaa aaacacggaa ttacatctag ccgtcaatac
    13561 cagaaaaagc tatcagaaaa tccaaattct gcaccaagta tgtggttcat taacaaaaaa
    13621 tatggatcat gggagaattt attgattagt ataggacgag aaaatactgg gtatggaaaa
    13681 tgggctagaa tgtcagaaca agaattattg gaaatagtag aagcctttat taagtgcgag
    13741 aagataacct ctcaacgaat gtacgaacaa aaatcagtag ggaagaatat tccttcactt
    13801 agtaccatta aaaagatgtt aggagatata cgtccacttt ttaaagaaaa aaatgatgga
    13861 tctcgattta ctgatttcga actgctgtta gaactaaaaa atgaaataat ccgattaaaa
    13921 ctacaggatg atttatcaat gacaaagttt cgaaaactag ttcaatcccc aaaactacca
    13981 tcagttgata caattatgaa aagaacgaac aaaaattggg aggaattgat ggcagagatt
    14041 ggatttgatt atcgaagaat caaaatttat aagcagagaa acaacctatc caagacaaag
    14101 aaaactaaat agtatcactc taaatcagac gttcaaaatt aaaatttgga cgtctttttt
    14161 attacccaaa aacgaaagga gatttatttt atgaaaaagc caagatttaa gaattggcgt
    14221 ttgctcgcaa cactgagtct attatgccaa acaattggtg gatcgcttgg cccaacgatt
    14281 gcctttgctg atgaaattac tcatccacaa acggtaactg tggaattgga tttggctcac
    14341 cagtatgctg tcgaaggtac atttagcgat ggtcgtccca tgtcagaagt taccgttccg
    14401 cactatgcag tttacaatgg tgtgaagcaa gatattttct gtattgagcc aggggtgccg
    14461 atttacaatg agttcactcc tggttatgag aaaaatccat tgcctgatat gtcggagaaa
    14521 gcgaaattag tttccgtttt atggaaggaa gctggtacgg atgtggatac acatattgtg
    14581 gcccagaaga tgatttggca agaagttaat ggctacacgc ttcactcaat taaacgttct
    14641 gatggtagtg cagtaaatat cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat
    14701 tatcaaaaga aaccaagctt ccataactcc acagtgaaaa ccgtgttagg tcaatcgact
    14761 actgtgacag atacgaacgg cttgaattta tcggagtttg atgaagttgt cgagaatatc
    14821 gcaaatattg attatcgtgt gaacggcaat caactagtga ttactccaaa tgccaattca
    14881 aaagagagtg gtgaattaac cctaaagaag tcagctaata caggaacacc ggtagcttat
    14941 aaaatggctg gtcaacaaac tttgatggcg ggggcaattg ataagcccaa tacctacaca
    15001 atcaaaattg atgtggaaac tgagggttct ttgaagatta aaaaagtcga taaagaaaca
    15061 ggaaatgttg taccgggaac cgttttccat ttagactttg gaaaaacatt acctgcaaaa
    15121 gacgtgacca ccgataaaga aggcattgcg acattggatg agattcctca tggcgcgaaa
    15181 gtaaccatta ctgaaaaatc agtgccagct ccttatacga ttgacactac accaatcacc
    15241 gcaaccatta aatctggtga gactatttct gtgacttcaa aaaatactcg cgaaaaaggt
    15301 caaatcattt tgaataaaat gggggtggaa acaggaactg atctttggaa cgacaaatac
    15361 tcattagccg gaaatacgtt tgccattcgc aaagacagtc caaccggtga aattgtccaa
    15421 gaaatgacca cggatgaaaa aggtcatgcg gaaacaccaa aagagattgc caatgcgttg
    15481 gaattgggaa cctattatgt gaccgaaacc aaagctagta atggctttgt gaataccttt
    15541 aaaccagtaa aagtcgaatt gaactatgtc aatcaaaccg tggctcttgt taccagtaac
    15601 gtaaaagggc aaaaccaaga aatcactggt gaaaccactt tgacaaaaga agacaaagat
    15661 accggtaatg aaactcaagg taaagctgag tttaaaggag ctgaatatac tctctttact
    15721 gcaaaagatg gtcaagcggt caaatggagt gaggctttta aaccagagtt agtgaaggga
    15781 acgaaagctt ctgatgaaac ggtgactttg actttagatg aaaagaatca agttgctgtt
    15841 aaacacttag cgattaatga gtattactgg caagaaacca aagcacctga aggatatacc
    15901 ttggatgaaa caaagtaccc tgtttccatc aaaaaggtgg atgataacga aaaaaatgcc
    15961 gtgattaccc gagatgttac ggcaaaagaa caagtcattc gctttggctt tgatttcttt
    16021 aaatttgctg gatcagctgc tgggactgcc gaaactggct tcaatgatct gacctttaaa
    16081 gtatcgccat tggaaggaac cagtgagatt acaggtgccg aagatgaagc aattacagct
    16141 tataacgagc aactaggttt tgatggctat ggcaagtttg aaaatcttcc ttatggggat
    16201 tatttacttg aagaagtaga ggctccagaa ggatttcaaa aaattacgcc attagaaatc
    16261 cgttctactt ttaaagaaaa taaagcggat tacactaaga gtgaatacat ttttacgatt
    16321 accgaaaaag gacaaaaaca accaattaaa acggtgaccg ttccttacga gaaactgacg
    16381 aataaagcat tttctgttag cttgaatcgt ttgatgctct atgatttacc tgaagaagaa
    16441 gatagcttaa cttctcttgg gacttggaaa gacggaggca aagaattgac agaccttgat
    16501 tctaccgagc tagttgataa attgagctat aacttgcatg aaatcaaaga agactggtat
    16561 gtggtagcgc aagccattga tgtggaagcg acaaaagctg cccaagaaaa agatgaaaaa
    16621 gccaaaccag tggtaattgc cgaaacaacc gcaacgttgg cgaataaaga gaaaacagga
    16681 acttggaaaa ttttgcataa attaaccgca gaacaagttt tggataaaac catcgtctta
    16741 ttcaactatg tgtatgaaaa caagaaaccc tatgaagcag gtgatgatcc agtagcgaaa
    16801 gatgctagct tgaacaatca agcccaaact gtcaattgta cggtggaacg ccatgtttcc
    16861 atccaaacaa aagcccacct agaagatggt tcgcaaacct ttactcatgg ggatgtggtg
    16921 gatatgttcg atgatgtgtc gattacccat gatgtactgg atggctcaaa agaagctttt
    16981 gaaacaattc tgtatgcatt acttccggat aatacgacca aagaaatttg gaaatctggc
    17041 aaaattgatt atgaagtgaa tgacaaagaa ttcactaaaa ccgtacttgc ggaaaaagtg
    17101 gacacaagta aatatccaga aggtacaagt tttaccttca aagaaattaa ctatggcaaa
    17161 gacggaaaca tcaatgggaa acacaatgag gatctaaaag aaaaaactca aactttaaca
    17221 cctaaaaaag ttccgacaac accaagtgca ccagaacaac cggaaatacc aactgttcca
    17281 agtgactctc aagaatctag tcctacagta aagacattcc cacaaacagg ggagaaaaat
    17341 tctaatgttc tgctgtttat tgggtttacc ttgatctttg cgactgctgg gtattatttc
    17401 tggaatcgcc gaaactaagg tgatgtgatg aaacaactaa ataggaaaaa ccaacgtgcg
    17461 cgaccgcctc ctaaaaaaag tggtcgcgct atttttgtat ccaaaactaa aaatgaaaag
    17521 aggaaaagaa gatggaattg aaatttgttg tgccagatat ggccgaaacc tttggaaaga
    17581 taaggtatgc cggcgaaggc gaagttttaa ctgaaggata tgggcggaat actacagtaa
    17641 ttggtcgtag ttatcatttg tattccagta agcaacgagc cgatgatatt gaagtggtgg
    17701 tagctgcgga agccggcgaa aaggattttg accaagatca accactaaaa gccgtgaatc
    17761 cccatttggt tgccaaaggc tatgagattg agaatcgtgg gtttaccgac tacgtgttgt
    17821 atgtcgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca gaaggtattg
    17881 ttgtagattc aggactaacg tttgggaaat tacgattttc cgcattacgc cgagaagtac
    17941 ggaagcaaaa tgaggatgga acggttagta acgaggtaaa agaacggact tataatttga
    18001 aatcctctgc gcaaggtcga atgatccaag ttagtatccc agcaaatgta cccttacgcg
    18061 agtttgccta tgacgcagag gtagagttgg tcaatccaat catggatacg gtcgccaatt
    18121 atgttttccg agaaggaact actgtcaatt ggttcattaa ggccgatgat ttagtcttga
    18181 aacgacaacc agatcaggga aattctacaa accaaaatga aggaaagaaa taggtgaagc
    18241 gtatggaaat taatgttgaa catattttag attgtctcga tcaatatgga aaaggtgagc
    18301 taacggagga acaactcact aaagcattaa cctatgatga aaaaatgttt ctgattatgc
    18361 atcaagggtt actggaagta ggtaacgatg tcgaagaaga ttttgatgta ctgaattggt
    18421 taggggaaga agagtccttc tttatggtta tcgaagtaaa tgaaagtcta tgtcgagaag
    18481 ccgaaaccgt attggaagaa attggtgtgg aaatgccgga tgccattgaa gtttttttaa
    18541 agcaactagt ggaaacgaaa caactcccta tagcagtaaa tgactagcat aactgcttga
    18601 aattggatca atgaaatcct atgaagcttt tttcgattgg aggtggaaac cataaaaatg
    18661 tacaaagggc atcgtatacg ggcaggagat cagcacttgg tttatcactt tgttcttggc
    18721 tggctactcg ctctgttcat cggttggatg agcgtctttt attttcaaga gcttagacaa
    18781 tttgatattt ctaagctatc actttctact atagaaatcg ttcgttctat aaaagattta
    18841 atctatttgc taggaagtct cgttctttca ggaagcacga tgttgcttta tatacacttt
    18901 tttcaggatc attggcgcag tttgtggcat cgacaaaagc tagcccggat gattctagaa
    18961 aatcattggt acgaagtaaa acaaactcag agtgaaggat ttttcaaaga cctcaatagt
    19021 agtcgaacca aagagacaat cagttacttc cccaaaatct attaccgtat gaaggatggt
    19081 ttactttcta ttcgcgttca aatttcactg ggaaaatatc aagatcagct tttaaagctg
    19141 gaaaagaagt tagaaagtgg tctatattgt gaactagtag aaaaggaact caaagactcc
    19201 tatgtggagt acactttact gtatgatata attgccaatc gaattgggat agacgaagta
    19261 gtggcagaaa gtggggcttt gcgattgatg aaaaatcaag tatgggctta tgattcttta
    19321 cctcatatgt taatcgctgg cggcacaggt ggtgggaaga cttacttttt actcactatc
    19381 atcgaggctt tattaaaatc tgatgccgag ctttttgttc ttgatcccaa aaatgcggac
    19441 ttagccgatt taggtacggt aatgcctcat gtctattccc aaaaggagga aatttctgct
    19501 tgtgtggaag atttctatga gcgcatgatg actcgtagca aggcaatgaa agaaatgccc
    19561 aactacaaaa caggagagaa ttatgcgttt cttggacttc caccaaactt tttaatcttt
    19621 gatgaatacg tggtttatat ggaaatgtta acgacgaagg aaagtgcggt gattttgaat
    19681 aagctcaaac aaatcgtgat gttaggtcgt caatctggtt tctttctgat tctagcttgt
    19741 caaagaccgg atgcgaaata tttaggagac gggattcgcg atcaatttaa tttccgggtg
    19801 gctttgggtc gtatgagtga actgggatat tccatgatgt ttggcgaggt tgataaaaaa
    19861 ttctttatga agcgtatcaa aggtcgaggc tatgtagata cgggagggag tgtgattagt
    19921 gagttttata cgccacttgt accaaaagga tatgatttct taagggaaat tagcgacatt
    19981 ggtggattaa acgttaatac ggaaggataa aataatagta tgtagcctag gagtgtcaag
    20041 aatcctgggc tttttttacg aggtgattta tgacattttt agatttattc gctgggattg
    20101 gtggcttttg cttaggtatg gaacaagcgg gtcaccagtg tatcggcttt tgtgaaattg
    20161 atgattttgc tcgccagagt tataaggcaa tccatgatac aagtaaggag gtggaaatgc
    20221 atgacatcac aagtgtatca gacgagttta ttcaatctct cggaccagtg gatatccttt
    20281 gcggtggatt tccgtgccaa gctttttcaa ttgcgggaaa gcggcaagga ttttccgata
    20341 ctcgaggtac tttattcttt gaaatcgctc ggttcgccgc tattctccaa cctaagtttt
    20401 tattccttga gaacgtccgg ggattactca atcacgaagg aggggctacg ttcgagacga
    20461 tcctccgaac gctggatgga ttggggtatg atgtggaatg gcaagtgctt aactcaaagg
    20521 cctacgttcc ccaaaaccgt gagcggatct tccttatcgg acattctcga gacgcatgta
    20581 ccgaacaagt atttcctatc attggatcgt ctccaacatc tgatcaaaac atcagaaact
    20641 tattaaatat caacccttcc aatcgaggaa tggggggaca agtctatggg tcagatgacg
    20701 tagcgcctac cttgaccggt gatgaaggaa tcaaaatcgc attgccggta gatgatggaa
    20761 tttctgtagc gggtatgtta ctgggaaatt ttgaacaagg caatcgggtt tatgagataa
    20821 ctggaacagc gcctactttg tcaacgaaac aaggcggaac gaagatcatg cttcggaaaa
    20881 acgaaacaga atatcaagag gtgaagccag ggaacagtgt gaacctggct tttcctaatt
    20941 ccacaagtcg tcgaggccgt ctgggaaaac aaagtgtgca taccttatta acaggagacc
    21001 aacaagcagt cgtcacggat cagtatcaaa ttcgaaagtt aacacctagg gagtgctggc
    21061 ggcttcaggg ctttccggac tgggcttttg atcgagcttc tcaagtcaat tcagatagtc
    21121 agttgtataa gcaggctgga aattctgtaa cagttccggt catttttgat attgctagaa
    21181 gattaaagga ggtaaaaaca gatgatttat gatgagttga ttggggaatc tactgggtga
    21241 ttggcaagat tcagtcagat ccggaattgg aagaagaact gtaccggctc aattttgata
    21301 ttcgcaaaaa cggggtgaaa gtccctgatg atccgtatgt aattgatgag gaaacggatg
    21361 ctcgaatgga gctgaatcaa gtcatcagtg aattggaacg aattgcggac tttgctaaag
    21421 aacctgatat ccgccaatac ttgtttgaga taaaagcaga gttggaaatt gagggtatta
    21481 ccgcggagta acggccaaat aaaccactga taggcactga ccgcggggga tttgcgaagc
    21541 agaaatcctc cgtggtgctg tgcctgttta tggacggcgt tagccggcca tgatgtctca
    21601 ccccccgtat ctaacagggg ggtacaaaaa cacaaaaaat aagcaagcaa cgataaggaa
    21661 tggctgtcct atcaaggata ataagcaatt tgtgtagatg ttaactgatt tgaaaaagtt
    21721 aacaccttaa atttggaggt gagaacttgg cacaaaggaa tttagattat cggctattaa
    21781 aagaccggcg taatgaatat ggcgtttcac aaaataaatt agctacgact tgtggactta
    21841 gtcgcccgta cttaaatcag atagaaaatg gtggcgtaac cgcatcgaca aaaaccatga
    21901 ggaaaatttt tgaccaactg gaaagtttca atcccgagtt acctttaacg ttactgtttg
    21961 actatgtaag gattcgcttt cccacaacgg atgcgaggaa aatcattcaa gagattctcc
    22021 atttgaaatt tgattatatg ctccacgaag attacgcctt ttactcctat caagaacaat
    22081 atgttatggg agatattgtg gtgatgttgt cacatgaaga agataaaggc gtccttttag
    22141 aattaaaggg tcgtggttgt cggcagtttg aaactttttt actcgcccaa aagcgtagct
    22201 ggtatgactt tttcgaagat tgtctaaaag ccggtggcgt gatgaaacgc ttagacctgg
    22261 caatcaatga tcgagttggc ctgttagata ttcctgattt aacgaaaaaa tgtcagaagg
    22321 aagaatgtat ctccttgttt cgtaccttca aaagctatcg ttccggggaa ctgttgaaac
    22381 ctgatgataa agatggtatg ggcaatactt tgtatattgg cagtcttaag agtgaggtct
    22441 atttctgttt gtacgaaaaa gactatgagc aatatatcaa gttagggatt cctttagacc
    22501 agaccgaaac gaaaaatcgt tttgaaatcc gactgaaaaa tgatcgagct tatcatgcaa
    22561 ttcaagattt gttaaaaggt cgcagtattg aaagtaccac attttccatt attaaccggt
    22621 acttgcgatt tgcggataag gtggaaggca aaaggcgaac taactggccc ttgaatgaac
    22681 aatgggggcg cttcattgga cggaatcaga aggaaattca attaacttct gaaccgaagc
    22741 cttataccat tgaacgaacg ttgaactggc ttggacgaca agtcgcccct acgtggaaga
    22801 tggccaagga gttggatcgg ttgaatcaga caacgtacat tcaggatatg gtaaataata
    22861 ctcgactatc agaccggcac aaaaagattt tggagcaaca aagtatggca attgaaaatt
    22921 tgattatatg aaaagaggaa tcgtgatgga aattgtgaaa gatattttat tggtgtttgg
    22981 tggcagtatg cttggtgtta ctgtgatgtg cttgatgcat gcaagtgcag cggcggaccg
    23041 agcaatggaa aagaaaggaa agtgatgaaa tgaatttcgg tcaaaattt
//
LOCUS       DAZBWR010000038        23015 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000038 DAZBWR010000000
VERSION     DAZBWR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.038"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHGT5_001299"
     CDS             complement(132..509)
                     /locus_tag="ACHGT5_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217295.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHGT5_001300"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHGT5_001300"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN7217296.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHGT5_001301"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHGT5_001301"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN7217297.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHGT5_001302"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHGT5_001302"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN7217298.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHGT5_001303"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHGT5_001303"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN7217299.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHGT5_001304"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHGT5_001304"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN7217300.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHGT5_001305"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHGT5_001305"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN7217301.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHGT5_001306"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHGT5_001306"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN7217302.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHGT5_001307"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHGT5_001307"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN7217303.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHGT5_001308"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHGT5_001308"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN7217304.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHGT5_001309"
     CDS             10661..11575
                     /locus_tag="ACHGT5_001309"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN7217305.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHGT5_001310"
     CDS             complement(11708..13273)
                     /locus_tag="ACHGT5_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217306.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHGT5_001311"
     CDS             complement(13266..14717)
                     /locus_tag="ACHGT5_001311"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN7217307.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHGT5_001312"
     CDS             complement(14731..15747)
                     /locus_tag="ACHGT5_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217308.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHGT5_001313"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHGT5_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN7217309.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHGT5_001314"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHGT5_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN7217310.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHGT5_001315"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHGT5_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN7217311.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHGT5_001316"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHGT5_001316"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN7217312.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHGT5_001317"
     CDS             21340..22764
                     /locus_tag="ACHGT5_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN7217313.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..>23015)
                     /locus_tag="ACHGT5_001318"
     CDS             complement(22806..>23015)
                     /locus_tag="ACHGT5_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217314.1"
                     /translation="KAFYISSAFFLFSNQTYNHLFLFKNVGVIQFCCKHLFYLHIVNG
                     RTNNGFFLVSLYRILFPKKKKQLEI"
BASE COUNT         7441 a         3868 c         5193 g         6513 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gcttt
//
LOCUS       DAZBWR010000039        22890 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000039 DAZBWR010000000
VERSION     DAZBWR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.039"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHGT5_001319"
     CDS             3..371
                     /locus_tag="ACHGT5_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN7217315.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHGT5_001320"
     CDS             551..1492
                     /locus_tag="ACHGT5_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN7217316.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHGT5_001321"
     CDS             1561..2292
                     /locus_tag="ACHGT5_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN7217317.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHGT5_001322"
     CDS             2436..4616
                     /locus_tag="ACHGT5_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN7217318.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHGT5_001323"
     CDS             4636..5118
                     /locus_tag="ACHGT5_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN7217319.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHGT5_001324"
     CDS             5134..5895
                     /locus_tag="ACHGT5_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN7217320.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHGT5_001325"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHGT5_001325"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN7217321.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHGT5_001326"
     CDS             7667..8152
                     /locus_tag="ACHGT5_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN7217322.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHGT5_001327"
     CDS             8145..8828
                     /locus_tag="ACHGT5_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN7217323.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHGT5_001328"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHGT5_001328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN7217324.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHGT5_001329"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHGT5_001329"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN7217325.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHGT5_001330"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHGT5_001330"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN7217326.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHGT5_001331"
     CDS             13125..13457
                     /locus_tag="ACHGT5_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN7217327.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHGT5_001332"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHGT5_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN7217328.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHGT5_001333"
     CDS             14283..15656
                     /locus_tag="ACHGT5_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN7217329.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHGT5_001334"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHGT5_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN7217330.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACHGT5_001335"
     CDS             16711..17151
                     /locus_tag="ACHGT5_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN7217331.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACHGT5_001336"
     CDS             17295..17996
                     /locus_tag="ACHGT5_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN7217332.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACHGT5_001337"
     CDS             17993..19114
                     /locus_tag="ACHGT5_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN7217333.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACHGT5_001338"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACHGT5_001338"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN7217334.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACHGT5_001339"
     CDS             20493..21401
                     /locus_tag="ACHGT5_001339"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN7217335.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACHGT5_001340"
     CDS             21436..21708
                     /locus_tag="ACHGT5_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN7217336.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACHGT5_001341"
     CDS             21719..22765
                     /locus_tag="ACHGT5_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN7217337.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7713 a         3986 c         5017 g         6174 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata
//
LOCUS       DAZBWR010000040        22650 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000040 DAZBWR010000000
VERSION     DAZBWR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.040"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..825
                     /locus_tag="ACHGT5_001342"
     CDS             13..825
                     /locus_tag="ACHGT5_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN7217338.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            822..1622
                     /locus_tag="ACHGT5_001343"
     CDS             822..1622
                     /locus_tag="ACHGT5_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN7217339.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1783..3051
                     /gene="rseP"
                     /locus_tag="ACHGT5_001344"
     CDS             1783..3051
                     /gene="rseP"
                     /locus_tag="ACHGT5_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN7217340.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3119..4828
                     /locus_tag="ACHGT5_001345"
     CDS             3119..4828
                     /locus_tag="ACHGT5_001345"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN7217341.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5040..9398
                     /locus_tag="ACHGT5_001346"
     CDS             5040..9398
                     /locus_tag="ACHGT5_001346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN7217342.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9543..10016
                     /gene="rimP"
                     /locus_tag="ACHGT5_001347"
     CDS             9543..10016
                     /gene="rimP"
                     /locus_tag="ACHGT5_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN7217343.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10039..11214
                     /gene="nusA"
                     /locus_tag="ACHGT5_001348"
     CDS             10039..11214
                     /gene="nusA"
                     /locus_tag="ACHGT5_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN7217344.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11236..11529
                     /locus_tag="ACHGT5_001349"
     CDS             11236..11529
                     /locus_tag="ACHGT5_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN7217345.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11526..11837
                     /locus_tag="ACHGT5_001350"
     CDS             11526..11837
                     /locus_tag="ACHGT5_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN7217346.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11850..14156
                     /gene="infB"
                     /locus_tag="ACHGT5_001351"
     CDS             11850..14156
                     /gene="infB"
                     /locus_tag="ACHGT5_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN7217347.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14183..14530
                     /gene="rbfA"
                     /locus_tag="ACHGT5_001352"
     CDS             14183..14530
                     /gene="rbfA"
                     /locus_tag="ACHGT5_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN7217348.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14687..15143)
                     /locus_tag="ACHGT5_001353"
                     /pseudo
     CDS             complement(14687..15143)
                     /locus_tag="ACHGT5_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15308..17670
                     /locus_tag="ACHGT5_001354"
                     /pseudo
     CDS             15308..17670
                     /locus_tag="ACHGT5_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18038..18961
                     /gene="truB"
                     /locus_tag="ACHGT5_001355"
     CDS             18038..18961
                     /gene="truB"
                     /locus_tag="ACHGT5_001355"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN7217349.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18965..19906
                     /gene="ribF"
                     /locus_tag="ACHGT5_001356"
     CDS             18965..19906
                     /gene="ribF"
                     /locus_tag="ACHGT5_001356"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN7217350.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20258..21043
                     /locus_tag="ACHGT5_001357"
     CDS             20258..21043
                     /locus_tag="ACHGT5_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN7217351.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21056..21907
                     /locus_tag="ACHGT5_001358"
     CDS             21056..21907
                     /locus_tag="ACHGT5_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN7217352.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22031..22483)
                     /locus_tag="ACHGT5_001359"
     CDS             complement(22031..22483)
                     /locus_tag="ACHGT5_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217353.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7683 a         3959 c         4952 g         6056 t
ORIGIN      
        1 ggaggaaaaa gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc
       61 gaatttcaat ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc
      121 aatggtcgtt gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg
      181 gaaacagtaa agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt
      241 tatgcctttt cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa
      301 ctacctgtcg acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt
      361 catgtcatgg gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca
      421 attgagcaaa caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagc
      481 agagcagaga tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata
      541 cacgcagaag aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct
      601 aaagagttac aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat
      661 tttctgttat ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat
      721 tttgatggtg cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc
      781 ggcggtgtaa aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtggtca
      841 cagcagccgt ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg
      901 agcttacagc agcaatctta gcagtaatag gagtttacga gctgtttcgg atgaaaggat
      961 tgacactctt aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc
     1021 caaaagaaag atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc
     1081 ttatagttat gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg
     1141 aagcaggttt tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca
     1201 atgcacgaac agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg
     1261 atatcggagc atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt
     1321 cgcccaacaa aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat
     1381 ttctttattt attgctatac ccagcaaaag aactatttaa tcatggtatg tttgtcatgc
     1441 ttctgatgac gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa
     1501 tcaaacgtca ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct
     1561 tagaccgttt cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt
     1621 agtaagtata cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat
     1681 tatattgata ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg
     1741 ttttataaaa aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca
     1801 tttatcatcg tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg
     1861 aaacgatcag gaatcctcgt tcgcgaattt gctatcggta tgggtccgaa aatttatggg
     1921 catcaagcaa aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt
     1981 cgaatggcag gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt
     2041 ttactaaatt ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca
     2101 aatgctatcc cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt
     2161 tatgtgaatg gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt
     2221 attgaaaacg acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct
     2281 aaattgtggc agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca
     2341 atcctgttat tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat
     2401 cgcgtaggag agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat
     2461 gaggtagtca gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt
     2521 acgaaaaatc cggataaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct
     2581 gtcgacgtga caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata
     2641 aaagccccaa tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc
     2701 agcggttcat tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat
     2761 aaattaggcg gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta
     2821 acaactgtta ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt
     2881 ccgattcctg ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg
     2941 aaaccattaa gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg
     3001 ttgttgatgg tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg
     3061 acaaataggg tataataaca aacgataact attttgagga aataaaggag tatatagaat
     3121 gagacaatca aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt
     3181 aagccatcaa atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc
     3241 atatctgcca ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt
     3301 tgaaaaaatc ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga
     3361 agaatccggg cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga
     3421 tcgaaaaatg atccttggac caactcacga agaaactttt actgatttga tgcgtaatga
     3481 aatcaattca tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga
     3541 cgaaaaacga cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcgta
     3601 ttctttccat gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta
     3661 caccgaagta ttcaagcgtt gtggcttaga atttcgctcg attatcggag acggtggcgc
     3721 aatgggcggg aaagactcaa aagaattcat ggcgatctca gaaatcgggg aggatacgat
     3781 ctgttattcg actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt
     3841 atcaaaaaaa tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa
     3901 atcaattgac gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt
     3961 attgttcatt gcggatgaac aacctgtcct tgttttggta cgcggagacc atgaagtcaa
     4021 tgacgtaaaa ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga
     4081 agcacaaaaa tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga
     4141 cgtaaaagta tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa
     4201 cgaaacagga ttccactata aaaatgtgaa tccagaacgc gatttcactg tactttctta
     4261 tgaagatctt cgttttgtac aagaaggaga tccttcacca gacaacaatg gtgttcttgc
     4321 ctttactcga ggaatcgaaa tcggacatat cttcaaaatc ggcactcgtt acagtgaatc
     4381 tatgggagca actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta
     4441 tggtatcggt gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg
     4501 aatcaactgg ccaaaaggaa ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa
     4561 agacgaatac caaacaaacc tgacgaatga actagaagaa gcgatgacaa aagctggcta
     4621 tgaagtactg gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt
     4681 gattggctgt ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt
     4741 gaaaatcaag aaaactggcg aaatggttga agtacgtaaa gaagaattgg tgaatacttt
     4801 accaatcctc ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct
     4861 gaatcagctg tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat
     4921 cgcagttcca attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa
     4981 aaagaagagg ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt
     5041 tgtcagaaaa acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag
     5101 aaaaaaatca tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat
     5161 cgcgtttatg ggaatttacg ctgcattttt ccaaaatttt gcctattatg ctatatcggt
     5221 cattgactca gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct
     5281 tagcagatga ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg
     5341 aaaatcagca atgcgataca ccactagcac agcaagtact gaaaacgcag gtacctgtga
     5401 taaaagatcg taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc
     5461 aacaatactt gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta
     5521 tcgaaccaga agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca
     5581 aacaagaaca ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac
     5641 aaaagaaaaa ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc
     5701 taggacgaaa tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag
     5761 aacgaagagt cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa
     5821 aacgcaagat cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat
     5881 tctcgaacgg cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa
     5941 aggtacgagg aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag
     6001 atatcataga agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag
     6061 tagagcttca tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc
     6121 ttgtggcaca agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg
     6181 cgcaagcctt tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg
     6241 gcgtggaagc gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag
     6301 cattgagtga agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat
     6361 atgacacgat catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct
     6421 ttgatgaatt tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta
     6481 tcacggatgg aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg
     6541 aattttcaaa agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga
     6601 atactagcta cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg
     6661 aattagctag atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa
     6721 aatttggtgt cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc
     6781 acttagcatg gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc
     6841 agctaaatga gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa
     6901 cgattttagc aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga
     6961 atgtcgaata cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg
     7021 aaaatctatt gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa
     7081 aaggggtaga agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa
     7141 aagcggtcta tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag
     7201 aaattatcca aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag
     7261 gtaatgtaca ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta
     7321 tgggcggagc gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg
     7381 atgagatgct aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag
     7441 aaaatacgaa taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt
     7501 atacaccgaa gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa
     7561 aacaaatgta cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga
     7621 attctattaa tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata
     7681 aaagtaatga agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg
     7741 cgacgatgac aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt
     7801 gccaatattc cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa
     7861 aacaatgtcc aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga
     7921 catttttagg attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt
     7981 atcaagcaga agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg
     8041 ccggaacgat tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa
     8101 gggacaataa tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg
     8161 gagtcaaacg gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg
     8221 atgtctatga ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa
     8281 cgactcattt tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc
     8341 atgatgaccc aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga
     8401 tccctacgga tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa
     8461 gtcaggaaca aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt
     8521 ttgttcgagg gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt
     8581 ccggactatc tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag
     8641 gagatgcgac actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc
     8701 atgctggatt agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat
     8761 ggaacaagat ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg
     8821 tgcccgactg gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg
     8881 ctgcagctta cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt
     8941 tgtattactg tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca
     9001 aaggaaaaga tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt
     9061 ccgtaaaaga aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag
     9121 gattcaaatt tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag
     9181 gtgatacgtt gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac
     9241 aaatcgttga agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg
     9301 gtaaagtttc aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac
     9361 cagatgaaaa tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt
     9421 gtaattttaa gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc
     9481 ggatttttcg ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac
     9541 ttttgagtag cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa
     9601 attttgaact tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat
     9661 ttattgacaa agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga
     9721 gtgaaaaact tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat
     9781 cctccccggg agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg
     9841 attatgtcca tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc
     9901 ttcaatcatt tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag
     9961 aattgacttt tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac
    10021 ggagaggaat cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct
    10081 gaaaaaggga tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca
    10141 tacaaacgtc actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga
    10201 aagatccatg tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa
    10261 gtctcattga aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt
    10321 tttgaagtaa caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt
    10381 ttacagcgtg tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa
    10441 aaagacatca tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt
    10501 ggtaaaattg aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca
    10561 catgatcgaa tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta
    10621 tttgttagcc gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa
    10681 gtctatgatg gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa
    10741 gtggcagttc gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag
    10801 ggacaacgag ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa
    10861 tggaatgaag atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat
    10921 gtgatcttcg atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta
    10981 tcgctagcaa tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat
    11041 aaaatcgata tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa
    11101 aaagaaacag aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa
    11161 tatgaaacaa ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag
    11221 taaggaggca aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt
    11281 gaaatgaaac ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg
    11341 atcgatccaa ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa
    11401 gtacaacaag cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat
    11461 gagttttatc aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt
    11521 ggaaaatgaa tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg
    11581 cgggaaaact aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa
    11641 aattggtgtt tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga
    11701 gttcatatta tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga
    11761 tcggaaaacc tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg
    11821 agctaattga aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa
    11881 aagaactcaa aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg
    11941 tgaaaaacca tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca
    12001 aaaacccaag cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac
    12061 agccgaacga tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc
    12121 gcagttatca agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa
    12181 caaaccaaaa taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg
    12241 gcaatcaaaa ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta
    12301 ataataataa ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa
    12361 ctaaaccagc tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg
    12421 aaggtatgaa cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta
    12481 aaaaattatt tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga
    12541 ttgaactgtt agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg
    12601 ctgatatcga taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc
    12661 cgccagtcgt aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat
    12721 tacgccattc acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct
    12781 accagatcga tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct
    12841 ttacaagtat gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg
    12901 cagatgatgg cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg
    12961 ttccaatcat cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta
    13021 tgcaagaatt aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg
    13081 ttgagatctc agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag
    13141 tcgcagaagt agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg
    13201 aagcacgtct tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac
    13261 ttcatgttgg tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa
    13321 acgatcttgg ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct
    13381 tgaatgatgt acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac
    13441 gtgcagcggg tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc
    13501 gtgtgacttt ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg
    13561 taatcatcaa agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga
    13621 ttgatgtgga aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa
    13681 gtgatgtaac cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta
    13741 caccgcaggc aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca
    13801 tctataaagc catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg
    13861 aagaaaaaat caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta
    13921 cgattgcagg tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa
    13981 tccgtgacgg aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg
    14041 acgtgaaaga agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca
    14101 tcaaagtaga tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac
    14161 tgataaaagg aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat
    14221 cttaaaagaa gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt
    14281 aacgatcaca gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat
    14341 tttatctgac ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg
    14401 attgattcgt cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt
    14461 tgaacttgat gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa
    14521 caaagactaa tcaaaaactc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg
    14581 ttttcattaa gacgtattac ctaagagttc catgacttta gtcttgcggc gtgtcagcta
    14641 accaaaaaag agaaaaagaa aagtaaaaaa ttcgactttc ttttttctag tagtatataa
    14701 atttttgcat atagataaag atcaagatac cgataacgga agctagattg taatctattt
    14761 tagtgacagt caggtatagc caagtaacta gaagatcagt ggaacctgca ccataaaagt
    14821 ttgagtttgt cggtccgttg acagttaaaa gaaaaatcac gttgaagtta ttgatatttc
    14881 cgattacgcc gcttcgattt ggtcacgatc caaattttgg ataatccctg tagaaattag
    14941 catagtagct gggataccaa ttcacatatt gacaaagata acagagactt ttacataaaa
    15001 cggatcaatc aggaaaggaa ttgctcgatc cagccccagt tttccagcaa tgtgttaact
    15061 ggcccagcgt cattcagcaa attatgcatg atgaggagcg aaatgaactg cggaacagcc
    15121 attgtgataa cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt
    15181 cgtggttttt tatacctgtt catttggcta gatataaaat ccaaacaaat gaacagaagt
    15241 gcttattagg tttcaattgg gagtatctgc gctagaataa gagtaataat gaaaaggagt
    15301 gttatgtatg gattattcta gtaaagaata ctttgataaa atgacggctt ggtggcgtgc
    15361 tgctaattat ctttctgttg ggcaacttta tcttaaagat aaccccttac tgagacgtac
    15421 actgaaaccc gaagatgtca aaaaacatcc aatcggtcat tggggaacga ttcctggcca
    15481 aaattttatt tatgtccatt tgaaccgagt catcaataaa tatgatttga acatgtttta
    15541 tattgaaggg ccaggacacg gcggacaagt catggtatcg aatgcttatt tagatggaag
    15601 ttacacagag atctatccag aagtaacgga agatgagaca ggaatgcaaa aattattcaa
    15661 acgattttca tttcctggag gcattgcttc tcatgcagca ccagaaacac caggttctat
    15721 ccatgaaggc ggagagcttg gatactctct ttcacatgca gtcggagctg tattagacaa
    15781 tcctgaagta atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc
    15841 tggaagctgg ttctcaaatg tctttatcaa tccagtgata gatggagctg ttttaccgat
    15901 ccttcattta aatggtgcaa aaatagccaa cccaacgatc ttggcacgta aatcagatgg
    15961 agaattagca aattacttta acggacttgg ctgggaacct ttctttattg aaggaaatga
    16021 ttcagaaaaa ttaaatcctg ttatggctga aaaaatggat caagcaatcg agaaaattaa
    16081 atccatccaa aaagaagctc gcctgaagac agcaacagat gtcgtgatgc caaaatggcc
    16141 agtattgatc gtacgcacac caaaaggatg gaccggtcca aaagaatggg acggggaacc
    16201 aattgaagga accttccgag cacaccaagt accgattcct gtagatcaag aacatatgga
    16261 tcatgcagat gctttactcc gttggctgaa atcctatgaa ccagaaaaat tatttgatgc
    16321 acaaggacgt attttagaag aaatcagaga aatcgcgcct actggtgacc aaagaatggc
    16381 aaaaaatcct atcaccaatg gtggaatcga tccaagaccg cttattatgc cagattggaa
    16441 aaaatatact ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata tgaccgaact
    16501 tggtaaattc gttcgcgaga tcattgagaa aaatccagaa aatttccgta tatttggacc
    16561 agatgaaaca aaatcaaacc gattgaatca agttttcaaa acgacaaatc gccaatggat
    16621 ggaaaaaatc gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg tcatcgacag
    16681 tcaattatcg gaacaccaag atgaagggtt cttagaaggg tacgttctga ctggacggca
    16741 tggttttttg caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac
    16801 ttcaaatgga tgcggaaatc acatgacctt tcttggcgaa atgattatcc ttcgctaaat
    16861 ctcattgcaa gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca
    16921 ggaatattga cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg
    16981 gatgcaaata cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat
    17041 ttgatcatct cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc
    17101 ttagtgaatg aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct
    17161 gaattggtca ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact
    17221 ttgctgctgg aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg
    17281 aaattacgtc atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat
    17341 tttacaaaag acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg
    17401 attttctttg atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat
    17461 atcacgacac catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa
    17521 gacgcagctt taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag
    17581 aaattacaag aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg
    17641 gattggaaat ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaatagaat
    17701 tttttacatt gattaagaaa ctgaaaaaat aaataaaaaa aacggaaaaa agactggaag
    17761 aatggccagt cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc
    17821 acccataaat ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa
    17881 aaaagcataa catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga
    17941 aaaataaaaa ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa
    18001 taattcagtt aaatcaaaaa tgaaatggag aaaagacatg gacgggttat tacctttatg
    18061 gaaagaacgc ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac
    18121 gaaaaaaatc ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg
    18181 tattggaaaa gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg
    18241 cgagataact ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac
    18301 aaaaccagta gatatgtctt tatctgaaaa aaaaatagat gaagtgatgg cttcatttat
    18361 tggtgagatc acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt
    18421 atatgaatat gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta
    18481 ccgttttatc cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg
    18541 cttcgaagta gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa
    18601 gaaacttggt taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc
    18661 ccagaaggat gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga
    18721 tgattgtttg ctgccaatag aaaaaggagt ggaacggttc aaacgagtag atattactga
    18781 tgaagtatgg cagaaagtaa aaaacggtat gagacttgat tatactgtat ttgcgctccc
    18841 agaaatgccg tcagaagaaa ttgctttgtt ttataaagga aaagttgtca gtatttatca
    18901 gccaaacccg aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata
    18961 acgaatgaat atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga
    19021 tgtcgtttta gtgctgggat ttttcgacgg cgtgcatatc ggacaccaga aagtcataaa
    19081 gacaggtatg gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca
    19141 gcatccatct attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct
    19201 taagcaaaaa gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt
    19261 tacttcttct tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt
    19321 acatgcaaaa tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga
    19381 tgtcgctcat tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga
    19441 agaagaacaa ggtgaaaaaa tcagctctac ccgtattcgt ggattactag acagcggaca
    19501 agtaaaagaa gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca
    19561 tggcgatgca cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac
    19621 ggttcatttg cctaaagaag gtgtttatgt gtcggaaata aaaattgggg aaacatggta
    19681 tccagccatg ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt
    19741 agagttgtat attttggact ttaatcaaga tatctatggt gaacatgtcg cggtacgctg
    19801 gaatcaattt ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact
    19861 caaggaggat gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa
    19921 aatctcttgt ttatgtataa ctagcaatta caataaaatg ggtgataaat aagcccagga
    19981 aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa
    20041 gtagcttctt cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct
    20101 ttctttatag aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat
    20161 ttgctttaga ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg
    20221 taataacagg tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa
    20281 agtcgcagta gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg
    20341 gaaagaaaaa atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc
    20401 agtcaaagca atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc
    20461 tgaagaagga atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt
    20521 ttgggttaat aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga
    20581 ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt
    20641 aaattacttt acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga
    20701 acagatccca tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt
    20761 taccgaaacg gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc
    20821 tggtgctatc cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact
    20881 cttggaaagt atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc
    20941 tgcatggttg gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg
    21001 tgggatgact ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg
    21061 tttattgatt gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa
    21121 gattggtggc agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg
    21181 cttactcgtt caattattcg cttctccagc tggtatttca gtgactacat ttttgattag
    21241 tttactttct ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt
    21301 gattggtgtt tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt
    21361 gattggtgtc ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc
    21421 agcaatcatt cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa
    21481 aaaaggcggt atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt
    21541 atacagtgca ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat
    21601 gatcggtgtc tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc
    21661 tgaagacaag agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc
    21721 ttatattttt tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa
    21781 cgtagttatc tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga
    21841 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
    21901 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
    21961 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
    22021 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
    22081 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
    22141 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
    22201 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
    22261 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
    22321 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
    22381 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
    22441 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
    22501 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
    22561 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa
    22621 ttcgttctct cctaaagtga tattatattt
//
LOCUS       DAZBWR010000041        21997 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000041 DAZBWR010000000
VERSION     DAZBWR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.041"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1167
                     /locus_tag="ACHGT5_001360"
     CDS             202..1167
                     /locus_tag="ACHGT5_001360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN7217354.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1266..2438)
                     /locus_tag="ACHGT5_001361"
     CDS             complement(1266..2438)
                     /locus_tag="ACHGT5_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217355.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2425..3570)
                     /locus_tag="ACHGT5_001362"
     CDS             complement(2425..3570)
                     /locus_tag="ACHGT5_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217356.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3584..4508)
                     /locus_tag="ACHGT5_001363"
                     /pseudo
     CDS             complement(3584..4508)
                     /locus_tag="ACHGT5_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4625..5704
                     /locus_tag="ACHGT5_001364"
     CDS             4625..5704
                     /locus_tag="ACHGT5_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN7217357.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5701..6329
                     /locus_tag="ACHGT5_001365"
                     /pseudo
     CDS             5701..6329
                     /locus_tag="ACHGT5_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6439..6666)
                     /locus_tag="ACHGT5_001366"
     CDS             complement(6439..6666)
                     /locus_tag="ACHGT5_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN7217358.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6769..9357)
                     /locus_tag="ACHGT5_001367"
     CDS             complement(6769..9357)
                     /locus_tag="ACHGT5_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN7217359.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9377..10036)
                     /locus_tag="ACHGT5_001368"
     CDS             complement(9377..10036)
                     /locus_tag="ACHGT5_001368"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN7217360.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10085..10588)
                     /gene="trmL"
                     /locus_tag="ACHGT5_001369"
     CDS             complement(10085..10588)
                     /gene="trmL"
                     /locus_tag="ACHGT5_001369"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN7217361.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10809..11675)
                     /locus_tag="ACHGT5_001370"
     CDS             complement(10809..11675)
                     /locus_tag="ACHGT5_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN7217362.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11784..12425
                     /locus_tag="ACHGT5_001371"
     CDS             11784..12425
                     /locus_tag="ACHGT5_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN7217363.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12487..13848)
                     /gene="mgtE"
                     /locus_tag="ACHGT5_001372"
     CDS             complement(12487..13848)
                     /gene="mgtE"
                     /locus_tag="ACHGT5_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN7217364.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13866..14765)
                     /locus_tag="ACHGT5_001373"
     CDS             complement(13866..14765)
                     /locus_tag="ACHGT5_001373"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN7217365.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14767..15564)
                     /locus_tag="ACHGT5_001374"
     CDS             complement(14767..15564)
                     /locus_tag="ACHGT5_001374"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN7217366.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15542..16246)
                     /locus_tag="ACHGT5_001375"
     CDS             complement(15542..16246)
                     /locus_tag="ACHGT5_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN7217367.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16377..16952
                     /locus_tag="ACHGT5_001376"
     CDS             16377..16952
                     /locus_tag="ACHGT5_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN7217368.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17070..17747
                     /locus_tag="ACHGT5_001377"
     CDS             17070..17747
                     /locus_tag="ACHGT5_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN7217369.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17826..19634)
                     /gene="pepF"
                     /locus_tag="ACHGT5_001378"
     CDS             complement(17826..19634)
                     /gene="pepF"
                     /locus_tag="ACHGT5_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN7217370.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19810..20808)
                     /locus_tag="ACHGT5_001379"
     CDS             complement(19810..20808)
                     /locus_tag="ACHGT5_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN7217371.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(21034..21687)
                     /locus_tag="ACHGT5_001380"
     CDS             complement(21034..21687)
                     /locus_tag="ACHGT5_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN7217372.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6492 a         4700 c         3814 g         6991 t
ORIGIN      
        1 cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct gctataataa
       61 ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca ttcaaaaaat
      121 agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat atcttgtttt
      181 attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa tccatcggca
      241 ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa aaataagcat
      301 attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat tgctgaaact
      361 ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat cgatactttc
      421 ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga tactttctat
      481 cagatggaag tagaattccc agttgcttat attccagcag gttctggaga tgattttgct
      541 cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt agcagctcaa
      601 tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata
      661 gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac aaatcattct
      721 tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt attctctatt
      781 ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg aggaaaacaa
      841 ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt tggcggagga
      901 atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa
      961 cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa aaaacaattg
     1021 aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc aacaacaccg
     1081 caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat cacaataacc
     1141 acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct acatctggaa
     1201 caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct ttttttacta
     1261 tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt acccaaaata
     1321 aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa
     1381 gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt attttgaaca
     1441 aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa gtattctgct
     1501 ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt gatcccaccg
     1561 ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat gatcggcaat
     1621 aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga ggtgtggatc
     1681 actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta actaatcaaa
     1741 ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg gtttcgttta
     1801 ctgatctctt tgcgattaaa tgctagattg atgacgctga aaccgctaaa aatggataga
     1861 aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt catccgctga
     1921 ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg tgtctgttcc
     1981 atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg
     2041 ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg tatcttgatc
     2101 gtcggcatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg taagataatg
     2161 tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc atccaatgta
     2221 acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg cgactcttct
     2281 tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc aatattttgc
     2341 gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt cgatttattt
     2401 gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc tttccatcca
     2461 agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat tggtataaaa
     2521 actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca agttgacgag
     2581 atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt gttccgaccc
     2641 catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc tatccttttg
     2701 caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa tcgcacacaa
     2761 cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt ctacttgata
     2821 gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg taaagcttgc
     2881 tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca agcgaatacc
     2941 atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca tcgtcccgta
     3001 gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca tattacgacc
     3061 tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg gcgcgagatc
     3121 aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta aaaattgatc
     3181 catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga gcgaaacaga
     3241 tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt cattcgcttc
     3301 tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga cttgttctaa
     3361 gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa ttccagtatt
     3421 aatcgtttcg agattgccac cttgatcaat ctcgccaaag gccgccataa acatcaatcc
     3481 taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca gcagtatttt
     3541 tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct ctcaaatcct
     3601 tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct atttttccgt
     3661 aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta tttagttcag
     3721 tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct attgttcgct
     3781 gacaacacgt tttctgggag taatgggatg tcgatcgtca aacgatccgc cgtccgtacc
     3841 atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc catgatcacg
     3901 atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac aatggttgct
     3961 ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg ggggtctaca
     4021 gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc acatgcgatg
     4081 ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac atatttttct
     4141 aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca
     4201 tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa gaccgcaacg
     4261 tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt cattgttgtc
     4321 ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca gttgatggca
     4381 gtcgatttcc cactgccgtt tggtcctaac agacctaaga cttctccttc ttgtacttgc
     4441 agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt atcaatttcg
     4501 ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa taaagtgcaa
     4561 gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa tgaaggagtc
     4621 tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta tctcattgct
     4681 tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga tcgccgtttt
     4741 attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc aagttattct
     4801 atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga cgatgcgagt
     4861 gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt tgttgatttc
     4921 gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc
     4981 cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt acattgtctc
     5041 caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag cattggccct
     5101 gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga
     5161 catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga
     5221 aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg ccaccgttca
     5281 cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat cgatctcttt
     5341 tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga ttgaaggaaa
     5401 cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca tcaaagaagc
     5461 cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt
     5521 gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa aaaattctgc
     5581 tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca
     5641 tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg agggacacca
     5701 atgataacag tagtcattat tgagatgatc catttgtgac gaaatcgtta caaaccattt
     5761 tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag
     5821 tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa
     5881 cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt
     5941 tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt
     6001 atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac
     6061 aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga
     6121 cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag
     6181 tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg
     6241 ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg
     6301 ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat
     6361 gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt
     6421 tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg
     6481 cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg
     6541 atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa
     6601 ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt
     6661 agccataaag taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa
     6721 agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt
     6781 tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg
     6841 gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc
     6901 tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg
     6961 gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg
     7021 tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata
     7081 agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt
     7141 caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc
     7201 ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg
     7261 aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg
     7321 tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata
     7381 agtgacctca tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt
     7441 tgccaagatg atacccgtga tttctcccat atctccattg aatacattca attcgggagt
     7501 attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc
     7561 cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat
     7621 ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt
     7681 atttttcgct ttttccacaa ttttagagat caagggttca atttgataag catcacttcg
     7741 gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat
     7801 ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc
     7861 cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga
     7921 gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa
     7981 taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc
     8041 ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg
     8101 atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt
     8161 tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag
     8221 tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc
     8281 tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg
     8341 cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc
     8401 tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat
     8461 cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt
     8521 tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg
     8581 gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt
     8641 atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct
     8701 cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat
     8761 tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt
     8821 cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc
     8881 catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt
     8941 tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat
     9001 agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa
     9061 tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata
     9121 tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg
     9181 ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg
     9241 gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt
     9301 ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg
     9361 aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg
     9421 tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa
     9481 tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg
     9541 tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat
     9601 ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa
     9661 tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca
     9721 gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc
     9781 ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga
     9841 taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa
     9901 catattttac ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag
     9961 aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg
    10021 taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa
    10081 ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt
    10141 gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga
    10201 catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt
    10261 ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat
    10321 tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta
    10381 aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat
    10441 ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat
    10501 gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta
    10561 atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt
    10621 ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac
    10681 tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat
    10741 aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc
    10801 tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg
    10861 tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc
    10921 tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat
    10981 tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca
    11041 cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc
    11101 tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt
    11161 tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca
    11221 gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta
    11281 gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat
    11341 tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct
    11401 tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt
    11461 ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa
    11521 taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta
    11581 tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc
    11641 aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc
    11701 ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag
    11761 tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta
    11821 ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg
    11881 gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag
    11941 atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg
    12001 aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag
    12061 tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg
    12121 aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc
    12181 ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg
    12241 gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct
    12301 atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac
    12361 atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt
    12421 tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt
    12481 gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact
    12541 cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag
    12601 acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag
    12661 catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag
    12721 aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac
    12781 aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc
    12841 tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa
    12901 aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc
    12961 taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc
    13021 attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc
    13081 aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt
    13141 aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc
    13201 gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc
    13261 attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac
    13321 atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga
    13381 gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt
    13441 ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact
    13501 taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt
    13561 atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt
    13621 catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag
    13681 ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc
    13741 atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc
    13801 ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc
    13861 tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg
    13921 aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct
    13981 tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag
    14041 ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca
    14101 actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct
    14161 tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata
    14221 tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca
    14281 aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa
    14341 agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg
    14401 taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat
    14461 tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca
    14521 tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga
    14581 atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca
    14641 ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct
    14701 ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac
    14761 tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg
    14821 ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc
    14881 ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat
    14941 ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt
    15001 attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc
    15061 gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc
    15121 tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc
    15181 ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata
    15241 actgatccga acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct
    15301 gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt
    15361 atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa
    15421 taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat
    15481 ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt
    15541 attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa
    15601 gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt
    15661 cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg
    15721 cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg
    15781 aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa
    15841 ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga taagaacggt
    15901 agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc
    15961 ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta
    16021 acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg
    16081 attttgttac aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat
    16141 gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata
    16201 cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta
    16261 cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt
    16321 tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga
    16381 cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca
    16441 tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata
    16501 ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc
    16561 aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct
    16621 ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag
    16681 tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc
    16741 ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt gatgaaagct
    16801 ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa
    16861 aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca
    16921 agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga
    16981 gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt
    17041 gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt
    17101 taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg
    17161 ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa
    17221 ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact
    17281 tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga
    17341 aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat
    17401 attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta
    17461 aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc
    17521 gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg
    17581 attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt
    17641 gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt
    17701 ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa aatacgtcga
    17761 ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat caaaaataga
    17821 aagccttata aacgatcgat caattcttct aattcattta gacgttgttc aaacatcgac
    17881 attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt cattacttcc
    17941 acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag tgcctctggt
    18001 tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt cgagtattgg
    18061 aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt aatttctggg
    18121 tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact atcacttagg
    18181 tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg ctcgaattca
    18241 gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg gttgagtaca
    18301 taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag gatattttca
    18361 ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac ataaggctgg
    18421 ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag cgtaaatagc
    18481 tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata gcttcccgaa
    18541 gaataagcat cgcttcgttt ccctttattt tcgacaacat cgatccaacg ttcagagaat
    18601 gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc taaagctttt
    18661 tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg tgtataaaga
    18721 tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat gtatcgatgt
    18781 aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact ttcaggaata
    18841 tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt cgctttaaaa
    18901 ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa ttgttcgtaa
    18961 acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct ttctagcaac
    19021 tgaccataca cgccatgaga taattggact atttcaccat tttctccttc aatcgttgga
    19081 aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc aaagatttca
    19141 cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg attatctact
    19201 atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa atattgccaa
    19261 atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc ttcactaact
    19321 ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt tccagtgtct
    19381 tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata aaccctcaat
    19441 acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc tttgtgtttt
    19501 tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc ttggtcgctt
    19561 gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg ttttggcaac
    19621 tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat caaaatagat
    19681 catagtctaa catccacatg tcacagcatc taacttttct actcattcat gtgtctgctt
    19741 ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc tagcctattt
    19801 ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa actgctgata
    19861 gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt ttatttggca
    19921 aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt acaacattcg
    19981 ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat agatccacga
    20041 tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg atactgtatt
    20101 cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac tcactttttc
    20161 tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt taaggtccct
    20221 actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag tctctgtaat
    20281 atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat gcactcctct
    20341 ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt gtccaagaaa
    20401 agaattacca agtatccacc atacttggta actgttattc aaataatttt gggttctttc
    20461 aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat tcgccaacaa
    20521 aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg gttctactga
    20581 ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg attctctttc
    20641 actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt ttttctttcc
    20701 ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc ctttttctgc
    20761 atgtttagca aaacagcact tattatattc attcattgca agcagcattt ttctccctct
    20821 gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa aaagctattc
    20881 tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc gtttggttgg
    20941 gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga ctgcgacaca
    21001 atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt tcgttcgctc
    21061 gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg gtgtacgatc
    21121 gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg cttggttgag
    21181 attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat acaaatcagt
    21241 ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa aatcttcaaa
    21301 agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt cttcctccgg
    21361 atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat caacagtaga
    21421 atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca gttctaatcc
    21481 gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact cctcttcgat
    21541 atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat tgccaagtaa
    21601 atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta cacggatcgt
    21661 attttcatta atatgttcca tttccatttt gctacacctc actttgccac aatgtttaat
    21721 atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg ctgttaagcg
    21781 aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa ctataacatt
    21841 tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc tatttttcgt
    21901 gcaatcatca tcttctacta cctttttata catctaatca agaatacttt ttgtaaaaac
    21961 aaaaaaactt ggaaaaacta tctttgtaat agtcttt
//
LOCUS       DAZBWR010000042        21974 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000042 DAZBWR010000000
VERSION     DAZBWR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.042"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1053)
                     /locus_tag="ACHGT5_001381"
     CDS             complement(106..1053)
                     /locus_tag="ACHGT5_001381"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN7217373.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="ACHGT5_001382"
     CDS             1299..1763
                     /locus_tag="ACHGT5_001382"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN7217374.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="ACHGT5_001383"
     CDS             complement(1825..2868)
                     /locus_tag="ACHGT5_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN7217375.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(3245..4210)
                     /locus_tag="ACHGT5_001384"
     CDS             complement(3245..4210)
                     /locus_tag="ACHGT5_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN7217376.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            4444..5289
                     /locus_tag="ACHGT5_001385"
     CDS             4444..5289
                     /locus_tag="ACHGT5_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN7217377.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            5480..6667
                     /locus_tag="ACHGT5_001386"
     CDS             5480..6667
                     /locus_tag="ACHGT5_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN7217378.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            6673..7029
                     /locus_tag="ACHGT5_001387"
     CDS             6673..7029
                     /locus_tag="ACHGT5_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN7217379.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            7031..7369
                     /locus_tag="ACHGT5_001388"
     CDS             7031..7369
                     /locus_tag="ACHGT5_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN7217380.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    7445..7701
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7764..8798
                     /locus_tag="ACHGT5_001389"
     CDS             7764..8798
                     /locus_tag="ACHGT5_001389"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN7217381.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            8791..9477
                     /locus_tag="ACHGT5_001390"
     CDS             8791..9477
                     /locus_tag="ACHGT5_001390"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN7217382.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            9501..10349
                     /locus_tag="ACHGT5_001391"
     CDS             9501..10349
                     /locus_tag="ACHGT5_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN7217383.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            10546..11319
                     /gene="sufC"
                     /locus_tag="ACHGT5_001392"
     CDS             10546..11319
                     /gene="sufC"
                     /locus_tag="ACHGT5_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN7217384.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            11332..12618
                     /gene="sufD"
                     /locus_tag="ACHGT5_001393"
     CDS             11332..12618
                     /gene="sufD"
                     /locus_tag="ACHGT5_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN7217385.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            12615..13850
                     /locus_tag="ACHGT5_001394"
     CDS             12615..13850
                     /locus_tag="ACHGT5_001394"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN7217386.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            13837..14307
                     /gene="sufU"
                     /locus_tag="ACHGT5_001395"
     CDS             13837..14307
                     /gene="sufU"
                     /locus_tag="ACHGT5_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN7217387.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            14312..15703
                     /gene="sufB"
                     /locus_tag="ACHGT5_001396"
     CDS             14312..15703
                     /gene="sufB"
                     /locus_tag="ACHGT5_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN7217388.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15798..16940)
                     /locus_tag="ACHGT5_001397"
     CDS             complement(15798..16940)
                     /locus_tag="ACHGT5_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7217389.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(16979..17713)
                     /locus_tag="ACHGT5_001398"
     CDS             complement(16979..17713)
                     /locus_tag="ACHGT5_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7217390.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17945..18220
                     /locus_tag="ACHGT5_001399"
     CDS             17945..18220
                     /locus_tag="ACHGT5_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217391.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            18318..20315
                     /locus_tag="ACHGT5_001400"
     CDS             18318..20315
                     /locus_tag="ACHGT5_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217392.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(20391..20726)
                     /locus_tag="ACHGT5_001401"
     CDS             complement(20391..20726)
                     /locus_tag="ACHGT5_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7217393.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            20854..21068
                     /locus_tag="ACHGT5_001402"
                     /pseudo
     CDS             20854..21068
                     /locus_tag="ACHGT5_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            21105..21467
                     /locus_tag="ACHGT5_001403"
     CDS             21105..21467
                     /locus_tag="ACHGT5_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN7217394.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            21513..21839
                     /locus_tag="ACHGT5_001404"
     CDS             21513..21839
                     /locus_tag="ACHGT5_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217395.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         7278 a         3878 c         4530 g         6288 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt
     3061 cgtaaattcc ttcttatttc ttgaagctga ccacttcttt tacgacctct tggtaaacgg
     3121 tgttcaaaag cttgcatctg cggtaataag cccaaccagt acaaaaacat gaacagctgt
     3181 ttttgtttgg ccgttcttat tacttgatgc agaacagctt tttcacaacc tattgaatcg
     3241 attattaagc gattttttta gaaacaaatt ttccatgttt gctatgttct ttaattaaga
     3301 aacgtacgta atgagcaaga acgtagaaga caactacact tgcaaacaga cattccgcaa
     3361 cttttaagaa atccaatacg ctactataga tacctaaact gttgaagtaa tgaacagtag
     3421 taaatatagc tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag cttggataaa
     3481 aacgaagctt gaacaaacgt ggaagcatag acagcacaac gaaatacaat acttgagata
     3541 aagcgaacaa tgctacgaca agataaaaat taggttcagc aaagttttta agataacctg
     3601 ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc ggatgagcca
     3661 tatttcttac gaaaaagaca cgtttgatga tgatcggcaa taagaacgca tagcagatca
     3721 atgaaatcca aaagaaaatt tttccaataa gtgggaagaa gttcccggca gttacagtga
     3781 tgaccccaaa tccaacgtaa atgataaacc agcttggata aattgcttca attgaaagat
     3841 cagctttgat aacatggtga taattgaaat aaaagacaag gatcacttgg aagataacag
     3901 cgcacagcca aatgaatttg acaattggtg caatactttc atatgaaaca aaataagtac
     3961 aaataaccat gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt ggatcatgca
     4021 acgtttgttt cgtgtgttcg aataagataa acaatttaac aaaaatgaat atcataagaa
     4081 tcatagagat accgccagtt agattgccta aagcaacatg gtgatagtct ttaaacagat
     4141 ttccaaggga agctaaccca aggatcaacc cgcagatcgg gatcggaata attttcaaaa
     4201 agttcttcat atataatatt ctccttttta tttaatagat tttagtacca agatgaacga
     4261 atacattgta aaatgaatag gaatgtatga ttacgagcaa gcagacagct gattgtcttc
     4321 atctaagtta ctagttggat cagcaactgt tttgttgtac atttctatta tagatggttt
     4381 tttgtatatt taaatcaaat agtttttata gtaattatca taaaaagtta tggaagtggt
     4441 gccatgttta agttattaac gacttttcga gcagtatatg aaacaagaaa tttttccaga
     4501 gcagcagaag tactgttttt atctcaacca gcagtctcga atcaaatcaa acagttggag
     4561 cgggaattga atatcgatct ttttcaacgt aacggaagaa aagagatgct tccaactaag
     4621 caagccgact tattatatga acggacactg gttttgttag aagaatggga agaaactcag
     4681 caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc ttcccacacc
     4741 tttgctgtct atcttttgcc tcagttgatg aaacatctga tcaaaaggtt tcctaaagtg
     4801 cattttgaga tcgaaatcac gaattctcat gaatcattag aacaagtagc gaaacatgaa
     4861 tccgattttg gttttatcga aaagccatta gagacttccg gcgtacaaag atactctttg
     4921 atggaagacc agctagtcat cgctggggac ccaaaaagca aattgtggct tgtaagagaa
     4981 gcaaactctg gtgtttttca ttacacgcag cgttattttg aagagatgaa tatccaagat
     5041 aagaagatga tcgtgaaaag taacgatgta attgttgcgt tgctgaagga aggaatcggt
     5101 cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc gttgaatttc
     5161 catcgatatt tttatttcat ccaacgaacc aatctggttt ctgatcagct gctagagatt
     5221 gccgaagaga tccttcgata caatgaaagc acagaaactt ctcacagcga ggagaatctc
     5281 agctggtaga taattgcatg tatcaaaaaa agttctatga tacactaaag ataagcagta
     5341 ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt tttcccaaat
     5401 gtctatggaa agactttcca ttatctaact ggaccgctat aataatcagc agtatggttt
     5461 ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata atcgtattga
     5521 ttatggcgtg attttaccag tctttctgtt atgtttgatt ggtctgctct cattatatgt
     5581 agcacttagc catgatccga acaatccagc agtcttgaaa ggcgtaggga tgcaagttgt
     5641 ctggtattta gttggagcgg cagcaatcgt tgtcatcatg catttgaatt ccaaatggat
     5701 ctggaaactg actccttacc tttatggttt ggggttagtg gtaatgggac tcttgctcaa
     5761 attctatgat cagaatttag ctgcttcaac agggtctaaa aactggtttc gattcggtgc
     5821 gtttactttc cagccagcag agttgatgaa gattgcgtac atcttgatga tggcactgat
     5881 cgtaactaaa cataatacgc aagtaaaaga acggacgatg aaatcggact tttggctgat
     5941 tgggaagctc ttgatcgtga caataccggt tttagcattg atcatggccc aagatgactt
     6001 tggaacgatg cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt ctggaatctc
     6061 ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc gttggagcag gaacgatctt
     6121 cttggttacg actgaaggcg gaagagactt gctttataaa gttggattca aatcttatca
     6181 gtttgcacgt attgattcat ggctggaccc tttccatgat acgtcaggaa tgagttatca
     6241 gccggcccaa ggacttttgg caattggtac cggtggcttg tttggtaaag gattcaacgt
     6301 aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta tcggtgaaaa
     6361 cttcggcttt atcggtggtg cattcgtcat tttcttgtat tttattttaa tctatcgtat
     6421 gattcgggtg tgtttcgata cgaataatga attttacgcc tatatcgcaa gcggtttgat
     6481 catgatgctg ctgttccatg tctttgagaa tattggagca aatatcggat tacttccttt
     6541 aacagggatc ccattgcctt tcattagtca gggaggatcc tcgattttag ggaacatgat
     6601 cggtatcggg ttgatattat cgatgagata ccaaaatgaa gcacctgcaa gatcttcaag
     6661 gaggtaagaa aattgtacac cttttactgg tacccgaaat gttcaacatg caaaaaagcc
     6721 aaagcgtggt tagaccagca tggcgttagt tatcaaaccg ttgatatgat caaagaagta
     6781 ccgaaggcct caacgattga gacttggcta gaaacgagtc aagtaccaat ccgtcgcctg
     6841 ttcaacacaa gcgggatgaa atatcgggaa cttggactga aagatcaagt aggcgagttt
     6901 acggcttcaa aagcgagcca agtcttatcg acagatggta tgctgatcaa gcgaccactg
     6961 atcttacgag aggaccagct tgttgcaatt ggattcaatg aaaaaattta tgaaggagaa
     7021 ctgatataaa atggcaggca agcatttgaa gaaaaaggac aacctttgga tcttatttaa
     7081 tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg gagacatcac
     7141 gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc tgatcgaagt
     7201 tgaagcggaa aaagcggtca atgaatttgc cagcccgcta acgggtacag tttcttctgt
     7261 taatgataaa atcggtgaag atatcaatat cttgaatgat tcagatgaaa tgaatgcgtg
     7321 gatcttaagt ttcaaagatg ttgatccaaa agaatttgac gaattataaa atcagaaaat
     7381 cacttgaaaa aagttgacac tgattcttcc agttgctata atcataagca atcctatatg
     7441 aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac gtttgaagag agccctgttt
     7501 gctgaaaatg ggtaacgaaa atgagatgga agatggtctt agagcagagt aagcgagcgt
     7561 aagcaaactt actacgggag tgcccgttac agcgcgacag tatacggaaa gaagctaagt
     7621 ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg taacacggaa
     7681 gttcaagctt tcgtcctttt gcttaaggca agagggcgaa ggcttttttg tttgaaaaat
     7741 tataagagaa agatgtgaat ggcatgtcat taatccaact aacgaatgtc aaaaagacat
     7801 tcaatgggaa aaacggaaac atccaagccg tcaatgacgt ttcactaaac gttgaaaaag
     7861 gggatattta tggaatcgtc ggctattctg gcgcaggaaa aagtacatta gtccgtctcc
     7921 tgaatggact tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa gatattacaa
     7981 aattaaaaaa caaagaatta cgtacattcc gaaagaaaat cggtatgatc ttccagcatt
     8041 tcaacctgtt atggtcaaga actgtacttg aaaatatcca gctgccgctg gaattagctg
     8101 gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact gttgcgttta gtcgggttag
     8161 aaggacgcgg acaagcatat ccttcacaac tttccggcgg acagaaacaa cgcgtcggta
     8221 tcgcacgagc attggcaaat gatcctgaga tcctgctttg tgatgaggct actagcgcat
     8281 tggacccgca aacaacagaa gaagtattag acttattgtt agcgatcaat aagaaattga
     8341 atttgaccat cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc tgcaacaaag
     8401 tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga cgtattgact gtcttcagac
     8461 aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga catcgaacca gaagctgatt
     8521 cgacagaact attagctgaa ctcatcaaag aaaatccaag cggccgtttg gcaactttga
     8581 cctttagcga aagaaatgcc aacgagccag tcatttccca agcgattcga cgctataaca
     8641 tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc tttggttcat
     8701 tgactgtctt gatgactggt gaaaccaaag aattagatca agcagaagtg ttcatcaaag
     8761 aacaaggagt aggaattgag gtgatccatc gtggataaaa gcttttcaga aacatatcta
     8821 gattttaaac aaatcaaccc ggaaacgatg aatcaggcta tccttgatac actatatatg
     8881 acattaatct caatggtctt agtgacgatc atcggattag ttttgggatt gatcctttac
     8941 tctattggac gcaaaaacaa accgtatgct cgaatcattt atggaatcgt atcgatcatc
     9001 agtaatattt tccggtcaac gccatttatg atcctgatgg ttttgatcat tccatttact
     9061 aaagcaatcg taggcactat gctaggtgcg caagcagcca ttccagctct tgttttatcc
     9121 gcagcaccat tttatgcccg tctagtagag attgcttttc gtgaagtcag ccctggtgta
     9181 ctggaagcag cagatgcaat gggagcaagt tactgggaaa tcatctggaa ggtcttgata
     9241 cctgaaagta ttcccgcatt gatttctgga ttaacagtta caacgatctc gatgatcgga
     9301 tttactgcga tggccggcgc gatcggcgca ggtggacttg gaggtcttgc ttggcaggaa
     9361 ggctaccagc gtggtaatct aacagtgaca ttcgttgcga ctttaattat tttactaatc
     9421 gtatttatcg ttcaaggaat cggtgatttc ttaactaaga aaacagacaa aagataatag
     9481 gatatagggg gatgtaggat atgaaaaaga aaatttttgg atttgcagca gcgcttttgc
     9541 tgactgtagg attagcagca tgcggtaaca acagcgacag caaagattca gcaaataaag
     9601 cagacgacac gacattgaaa gtcggcgctt caccgacacc gcacgcagag attttggaac
     9661 atgtgaaacc attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa ttcgatgact
     9721 atgtattacc gaaccaatct ttggaagaag gcgacattga tgccaattac ttccagcata
     9781 ttccttattt gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat gccggtgctg
     9841 tccacatcga accaatgggc ttgtactcta aaaaagtcaa agatgtttcc gaattaaaag
     9901 atggtgcaac aatcattact tcaaattctg aatcagactg gggacgcatc atcacaattc
     9961 tacaagatgc tggcttagtc aaagtcaaag atggagtaga tctggaaaca gcaacattcg
    10021 atgatattga agaaaaccct aagaacttga agttcaacca tacgatcgac ccatcacttt
    10081 tagcttctac gtaccaaaat gatgaaggtg acctagttgc aatcaacgca aacttcgctt
    10141 acggcgcagg gttgaatcca ttagaagatg ccgtattgct tgaaaaggac aactctcctt
    10201 acgtgaatat tattgcgact cgtaaaggtg atgaagacag tgacaaaatc aaaaaactga
    10261 ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac agacaaatgg ggcggctctg
    10321 ttaaaccagt aaaggctgat gcaaaataag gtatggaaca aaaaagatga gaaataagtc
    10381 tgtaatggct tatttctcat cttttttata cctattctaa tttagaaatc aaaattgttt
    10441 aataataaat aaaagatatt tgattctagt aaggaaggtc attgcgcttc attctcaatt
    10501 agtctaaaat gagtgagaac gaaagagaat ttgggggtat aatgcatgtc tgtcttagaa
    10561 ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc ttaaaggcgt aaacctaaca
    10621 atgaaaaccg gagaaatcca tgccatcatg ggacctaacg gcacaggaaa atctacctta
    10681 tctgccgcta tcatgggcaa tccaaactat gaagtaacag aaggcgaaat tttgtttgac
    10741 gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc gacttggctt atttttagct
    10801 atgcaatatc caagtgagat accaggtatc acgaatgcgg aattcatgcg tgcagcgatc
    10861 aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg catttttgaa aaagttagat
    10921 gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg aacgttatct gaacgaaggt
    10981 ttttctggcg gagaaaagaa acggaatgag atcctgcaat tgttgatgtt agaacctaca
    11041 tttgcaattt tagacgaaat cgattcaggt ctggatatcg atgctttgaa agtcgttgct
    11101 aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat tgatcatcac ccactatcaa
    11161 cgattattga actatatcac acctgatgtc gttcacatca tgatggaagg ccgagtagtc
    11221 atgacaggga atgctgacct agctaaacgt ctggaagcag aaggctacgc tggtatcagt
    11281 aaagaattag gaatcgacta taaagaagaa gaagcgtaag ggggagcaag aatgaaagaa
    11341 aaaaactggc tcgattatct tgacgcggta aatgattttt ctttaagtaa aggcgaacct
    11401 gattggatga gaaccttccg tcaagacgca ctagcgaaag cagatgaact gccattgccg
    11461 catatcgatc gggtgaaatt ccatcgctgg tcgttatttg atgtgaaaga aacacaaacc
    11521 atcagtgaaa caggcacgat tccggcattt gatgcaatga aagacaatcc tgtgcttgtc
    11581 caacaaggtt catggacgat ttttgaacaa ctgcctgtgg aattagcaga aaaaggagtc
    11641 atttttaccg acttgttcac tgcaatgatc gaatatccag aactagtcca agaatattat
    11701 atgaaaaaag cagtaaacat gaacgaagac caattgactg cactccatgt agcttttatg
    11761 aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga tcgatgaacc actcgagtcc
    11821 ttattcattc aagatggcgc atccgatgag cacttcttca agcatgtgtt gatcgtggca
    11881 gatgaacata gtgaattttc ttatctggaa cgtttccaga caaccaaaga acaagtagca
    11941 aaatcatcag gtaatatcat cgtggaagtc atcgctaaag ctggttcaaa aatcaaatat
    12001 tcagccgtag atcaattagg agaaaatatt acttcttata tgaatcgccg tgggcacatt
    12061 ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga atgacggtca tgtgatcgct
    12121 gactttgact cagatttagc aggagaaggt gcacacgcag aagtcaaaat cgtggcaatc
    12181 agctcgggca gacaaatcca aggaatcgat acacgagtaa ccaataaagc ccctcatacg
    12241 atcgggcata ttttgcaaca cggagtgatc cgagaaaaag gaacattgac ctttaatggt
    12301 atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc aacaagaaag tcgtgtcttg
    12361 atgctttctg ataaagcacg tggtgatgcg aatcctattc ttttgatcga tgaaaacgaa
    12421 gtaactgctg gacatgctgc cagtgtagga cgagttgatc cagaagaaat gtactatctg
    12481 atgagtagag gattgcataa agaagaagca gagcgcttag tgattcgagg attcctcggt
    12541 tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag aattagtcga agtaatcgaa
    12601 gggaagttga atggatgatc gatgctaaaa agctacgcaa tgattttcct atcttatttc
    12661 aagacgtcaa tgatgaacct ttagtttatc tggataatgc agcgacgacc caaaagccaa
    12721 aacaagtact ccaggcatta gcgtactatt acgaacacga taacgccaat gttcatcgcg
    12781 gtgtccatac gttagcagaa cgcgcgaccc atgattatga acaagcaaga gaaaaagtac
    12841 gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt tactcgtggt acgacgacaa
    12901 gcttgaattg ggtagccaga accttcggcg accgctatat caaagcagga gacgagatag
    12961 tgctctctta catggaacat cattctaatg ttatcccgtg gcagcagttg gcaaaacgaa
    13021 caggtgctgt cttgaaatat atcgaactaa cagaagaagg cttcctggat atggaggatg
    13081 cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat tgctcatgta tccaatgtct
    13141 taggtgtctt caatcctgtt caagaattag ctgaacttgt ccatgaaaac ggcggggtca
    13201 tcgtagtaga tggtgctcaa tctgccccgc acatggccat tgatgtccaa gcactagact
    13261 gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc aacaggtatt ggtgttttat
    13321 atggtaaacg aaaatggtta gaagaaatgg aacctgttga atttggtggc gaaatgatcg
    13381 attttgtcga attatacgac agtacatgga aagaactacc ttggaaattc gaagcaggga
    13441 caccaaatat cgccggcgct atcgcattag gccatgcaat cgattatttg caaaaactcg
    13501 gtatggacaa tatccatcga tatgaagaag aactagccgc ttatgtgttg ccaaaattac
    13561 aagcaattga tggattgact gtttatggtc cacaagaccc gaattttcgg acaggagtcg
    13621 ttgcattcaa tttggatggg ttacatcccc atgatgtcgc gacagcttta gacatggaag
    13681 gggtagccgt cagagcaggt catcattgtg cgcaaccgct attgaagtac cttgaagtat
    13741 cagcgactgc tcgcgcgagc ttttatttct acaacacaac gcaagacgcc gaccgtttag
    13801 ttgaagcaat catggcaaca aaggagtttt tccaacatgg gactatctaa attagacaat
    13861 ttataccgtc aagtgatctt ggatcattct tcccatccgc atcatcgtgg aagcttgact
    13921 gactcgacaa gcaagatcga attgaacaac cccacatgtg gagatgtgat cgaattgcaa
    13981 gtcgatgtaa aagacggaat catcaaaaat atcgcctttg acggcagtgg ctgctccatc
    14041 agtacggcaa gtgcctcgat gatgacagac gccgtcatcg gaaaatcaat agaagaagca
    14101 gaacatttaa ctgtagactt tctacagctt gtccaaggaa aagaagtcaa agatcccgac
    14161 caattaggcg atgctgctat gcttggtggt gtagctaaat ttcccgctcg gatcaaatgt
    14221 gcgacacttg cctggaaagc actaggaaaa gcaatggaaa acgatggtac cgcagctttc
    14281 catcattacc acaaggaaga ggagtgagaa catgggcgta ccagaattag aagaatataa
    14341 attcgggttt cacgatgatg tcgaaccggt tttcagtaca ggagaaggct tgacagaaga
    14401 agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa tggatgcttg aattccgtct
    14461 gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg acctttcaga
    14521 catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg ctcgtgactg
    14581 ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac cagaagctga
    14641 acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt accataatat
    14701 gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg ctttgaaaga
    14761 ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg acaacaaatt
    14821 agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc caaaaggcgt
    14881 caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca cggggcaatt
    14941 cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg aaggttgtac
    15001 ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct ttacaagaaa
    15061 agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt acaacttagt
    15121 aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg gaaacctagg
    15181 tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg cacgaggcac
    15241 catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg caaaaatgat
    15301 ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa aagacggcgg
    15361 agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt cgatctctca
    15421 tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga tcccattcaa
    15481 tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa aaatctcaga
    15541 agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa ctgaaatgat
    15601 cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg cagtcgaact
    15661 caatcgattg atcagttatg agatggaagg cagtgttggg taaacctgtt ataggagcta
    15721 gaacccttga tatataaggg ttctagcttt tttgttttgc ctgtatacta gaaatgacca
    15781 gattttgacc agctctttta ctgatgagcc atcatatcag caaagtcttg acctgttttc
    15841 ttaggtgcat tatttgtaat atgtgcatag atgtctaatg tcactttact attttcatgc
    15901 cctaatcgtt gttgaactac cttgacagat tctcctgctt ctaataataa actagcatgt
    15961 gtatgtctaa acccatgagg tgtaatacga ggcaagttat attttttcaa aatatatctt
    16021 aaccaatcat taacagtgtt tggaatgtat agagtgtttt taactgttgt aaaaacatgt
    16081 tgctctttag agctagtatt gtatccaagt tttaaatact cttctttttg ctgtaatttc
    16141 cagttgttga ggatagaaag agtctcagta tctaaagaga taactcgtct agaagctctt
    16201 gttttcggag attgaattat aactttacca aattcatcta cagcaagagt tttattgaca
    16261 tgtagttctt tattaaacgt gtctatgtct ttccactgta aagcgagtac ttcactcttt
    16321 ctcatacctg tgtatgccaa taatctgaag aaggcgtatt gtttcatgtt accaaaatct
    16381 ttaaaagcat ccagtaaatt attaagctcc tcttttgagt aaaatttacg atttttctca
    16441 tactcttttt ttcgtggaag aagtgttttt tccataggat tattgtcaat atattgcatt
    16501 gcaaccccgt agcgtaaaat ttgtgcagtc tgttttctca tataatcgta actttcatat
    16561 ttactgtgcc attcatctac cactttttga caatatggga cagagatatt cgatagttta
    16621 agcttaccaa atgctggaag tatttgatta tttgcgtatc ttactgagat tgcgattgtt
    16681 gaagctttaa cactggtacg ctgatgagtt atccattcat tatacaggtc ttcaaatgtc
    16741 atatcaggtg ctttcttttt aacaccgtat aatatttgat gtctgtactc gtcataatct
    16801 ttttgagctt cttgtttgct actaaaacct cttctcgtag tatttatttt ttcaccagtt
    16861 cgtggatcgg tggcaatgtg tcctttatac atccacgcca cttgaccgtt tttctttttg
    16921 tactttgata tttttgccat gaaatcatct cctcttagtt tacaaggagt acttaccact
    16981 agatggtatc acctcccttc atttttttct tagtgtattt tttcccaatt gatgaaatcg
    17041 tttgttctag cttgtttata ttatcagtaa tttctctcac agcttcttcg ggataagaca
    17101 tgtctttcaa ttgaataggt agccattcat ctaccattct ttctagtagt tcttctatac
    17161 tgctagtgag gaaaagaatg ttattgtcaa attcttctac aatagcattg atgaataatt
    17221 cttctatttt taagatattg aaaagactaa ttttttcatt tttaacttta ttacaagtct
    17281 tgttagcaat aaattcttgc atctctggtt ctaatttgga atatctatcc agccatgtat
    17341 ctaaattaga gtgtactggt gggaactgtt gacgaagatg ctggaacagg tttttatctg
    17401 gaaatgttat accatctagt acaatgctat ctctataaag aataattccc aaaatatatt
    17461 cttctacatt gccacaaact aactcctcta tactcatatt tcccaaatca gcaattcggg
    17521 ataatcgctc tggtattggt aaataggttc ccttctccca ttcgtagata gtttttttgt
    17581 caattaattg accgttgtta atcataagac caaatgctgg ttgtgttaat tttttactct
    17641 ttcttatagc ttttattttt gttccgagta tcttttttcg ttctaaataa tttattggtg
    17701 gacgttttat catattgacc acacctccta aaattagtat acctcgaaaa aggggtgttg
    17761 taaataaatt ttacaaagat attgacacct cttttttatc gaggtattat gtgtatgtcg
    17821 atgaactggt aatcattttt tgtttgatta atcggttaat aacaaaatac ttttattttg
    17881 agcaaaaaat acgaagataa ttcttagaaa atttgttcaa gcaatcacga aaggaggggg
    17941 aaatatgtct ctacaaatca ttgatagcat tgtttctata caagttgaac aacaagtgaa
    18001 agagataatt acagtgctaa agcctatgct agaagacatt atttctggag tcactctaga
    18061 gtatatggac tatcagcagg caagtgaata tctaggtgta tcagtaggaa cgattagaaa
    18121 atatgtttct caatacgggt tacctgttat aaaaattgat acggtcatta gatttaaaaa
    18181 atctgatatt gatagtttca tggaacggta taaaggttga gtaaacaaat aagactaaaa
    18241 catatagtaa taaagagttt gtttttatta tctgtatgtt ttagtcttta acagaatttt
    18301 ataaggagga aacacaaatg gaaacatcaa tgattaatat tccaatttat gtagataaag
    18361 aaaaattatt agagaagcca gttacaaaaa aagaaaaaac tgatgtaaga gataggtgtt
    18421 tagataacta tagagaagtt acaagagatg agtttgtaga gatactaaat tcatcacaat
    18481 catttattcc aagcaagaca aagaataaag gcaatgacaa ggagagtttt gtggaaacca
    18541 gagtaattat tctggatgtc gataacactg ttaaagatga gaaggggaag gtagtagatt
    18601 tgagtaaaga tgatagtaga tacttaagca tagaaaaggt tttatctatc aatttagttc
    18661 ataattctgc gtttgcgata caaaaaagta ttcggtactc tgaaaatcta gaaaaataca
    18721 agattgtctt tctattaaaa gaagcaatta ctgactatgg taaaatggtt gctgtttacg
    18781 aatacttgaa aaagaacatc cccgggtgtg atgacaatgt aaatgcttcg aatagaatgt
    18841 tttttggagg atataagtca gatgcagtga taataattaa tgaagacaac atgttagata
    18901 ttactgagct tccgattgat tttaccatga catgttctaa aaattgtcaa agtgattcag
    18961 ggagagagta tgaactagtc aatgaaaccg attttgttaa gttgataaaa aatgacgaca
    19021 aagaagaaat gaaacagtgg tttaaagact gcctgtttga ttcctccgac atgtctttta
    19081 atgagattta tgaaagattg ttaaccattg atatgaataa attacttaag tcaagtaaaa
    19141 acttaagatg tctgtttcac aacgatcata atccctctgc tagtattttt accagtaaaa
    19201 ctgggcattc cattttttat tgtcattcca gtaattgtga tatcagtgaa aattttattg
    19261 gagttgtgat gctaatttta aagaaagaca gtcgggtaga aacatttaat tggctattag
    19321 agaacctaga cttgtatcct acacattttc gaaaattgaa agaggaatca aaagtacttt
    19381 ttgacactct tgaatcaatg aaagacaaac ttttcaggac aataaggaat tatctagagg
    19441 aaatgcgacg tgtctatgat gtcttgcttt ctgatgttaa tttttttgat gatagtaagg
    19501 aaagtgtgac ttgtattttg tcgggggaac aattagctaa aaaaatgagt ttttactatg
    19561 gtaaaactta tgacatagat aaatgtaata agttactatc acttatgact tttataggat
    19621 tgataaaaaa attagatgat gaagatgttc ctttgaagat gctcgaatac ctaaataggt
    19681 taaaggaaga gaaagcgtta ggaaacttta atcataagag aagcaatgtc tatcgattag
    19741 acgtttttaa tgcaacttct gtgattcaaa aattgaatga agatatcctt ccacttctgg
    19801 aaaaatttca ttttacatat caatattttt cttatgaatg ggttaagata tgttttaacg
    19861 aaaaagaggc taaaagagta tttcctcaaa attcccgatc agaacttagc aaggaaaaac
    19921 aattgatttt agatgaagca catgaagttt tacagggctt atcgtggcaa ggaacatatc
    19981 taatgacaga gaaagaactt actaacactg tttgtgaacg tctacgtaat gttgggaata
    20041 acagcgtgga aaatgcttta aaagaaaaca gaggctactt tgttaaacaa ggattcactc
    20101 ttgaacgagc ttctaaggat attaagagac tatacaatgt gaaagcaagt agtagttttt
    20161 tagtctacct agcaaaagga gcagggatag atgaagtaat aaaagaattg atattatcag
    20221 gagttgaatt agttcctctt tatcaattta caagtgtaac aagagatgat ggacggagag
    20281 taaggaagag aaaagaccca attgtattaa actagacagg ttatccatag ctcttaatct
    20341 tatttcatct caatacaccc accgttaaag ttcggtgggt tatttatttt tcaatctttc
    20401 aatagctgat tgcgttcatt ttcttgcctt tttaatagct tcatatagtc aattgttcga
    20461 actgcatctt cagtgctaag agaacgatag agagccaaaa actcttcctc ataatctacg
    20521 acaacattgt ctagaaaatg gcttacaggg gttcctaggg ctctagaaag agcggttaag
    20581 ctatcgaggt taggctctgt tacagaattt tcccatcttg ctactgcctg ttgtgtagag
    20641 aagatttttt cagctaattc tttttgtgtc cacccgagag cttttcgctt ttctctaatg
    20701 acctttgcat attgtttctg tttcattaat acacccccat ctcagtatag tacaacaagt
    20761 tgttgtattt gtaatggtat ttgaagtttt tttatatttt atgagttgaa atacatcata
    20821 ttgttgtata taataaaaca ggaggggaga atcatgaaaa aagtaagtaa tctcagccga
    20881 tacagaaaag aaaaaggact ttctcaaacg gagttagcaa aaaaatgaat gttacacagc
    20941 aatgtgtaag ttcgtggcaa acagggagaa cgattcctaa accctatcaa atgaagatgc
    21001 tttctgagat attaagtgta ccaatgaatg agctgttttc tgatgtattt aataaagtaa
    21061 acagttaact aataaacaat tgaatgtttt taggaggaga agacatgtat tgtagaaaat
    21121 gtggcaaaga aataacagat gaaagtaagt attgccagtt ttgcggagca ctacaagaaa
    21181 cagatgagac aactggagca gacgcagtaa agaaaagtac tgagcagaca attaatgaaa
    21241 tgaaggaaca aatcgtacat ggacaagaga cagggaagct gtatttgatt attggttgga
    21301 tttctatggt agtagcgtta gcatttattc ctgttgtatt tggtgctgtt gctgttatta
    21361 tgggctactt atatcgagat aaagatgaac aaatgggtac gattttgatg atagcagggg
    21421 tagctggagc aatctttggt gtacttttag ggatgtcaat gtactgataa gagggataag
    21481 agcaaattat tttggagata ggagataaaa agttgaataa agtaaatatg aacaagaagt
    21541 ttatcattat agttatcgtt gtcttggcta ttataggtat cattttttca aaaatgacag
    21601 gtacatctgg attagagaag tcatcgaaac cattggtaac acagatttta caagaacagt
    21661 acaacttaaa tcgatcatgt gatgatgtga cgattactca tgaaaatgga gataacacat
    21721 accgggctaa agctatatta gacaatggaa gtgctatcaa cataaatatt gagtactatc
    21781 caaagaaaga ccgtatatat gtggagatac catatgctga ggtgttaatg ttaaattgat
    21841 ttaaatgtat tatgcatacc gtccaaacag gaactcccaa aggtgtaaat tcactaggga
    21901 gtttttatag tttcctataa attaaatact aatacaggtg acaaaaaaat attacaatga
    21961 ttgtcgggaa tttt
//
LOCUS       DAZBWR010000043        21820 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000043 DAZBWR010000000
VERSION     DAZBWR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.043"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..1466
                     /locus_tag="ACHGT5_001405"
     CDS             126..1466
                     /locus_tag="ACHGT5_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN7217396.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1684..2127
                     /locus_tag="ACHGT5_001406"
     CDS             1684..2127
                     /locus_tag="ACHGT5_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN7217397.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2631..4049)
                     /gene="nhaC"
                     /locus_tag="ACHGT5_001407"
     CDS             complement(2631..4049)
                     /gene="nhaC"
                     /locus_tag="ACHGT5_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN7217398.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4452..5675)
                     /locus_tag="ACHGT5_001408"
     CDS             complement(4452..5675)
                     /locus_tag="ACHGT5_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN7217399.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5706..6545)
                     /locus_tag="ACHGT5_001409"
     CDS             complement(5706..6545)
                     /locus_tag="ACHGT5_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN7217400.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6562..7242)
                     /locus_tag="ACHGT5_001410"
     CDS             complement(6562..7242)
                     /locus_tag="ACHGT5_001410"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN7217401.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7244..8317)
                     /locus_tag="ACHGT5_001411"
     CDS             complement(7244..8317)
                     /locus_tag="ACHGT5_001411"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN7217402.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8373..8611)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8654..8887)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8964..9635)
                     /locus_tag="ACHGT5_001412"
     CDS             complement(8964..9635)
                     /locus_tag="ACHGT5_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7217403.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9722..12370)
                     /gene="mgtA"
                     /locus_tag="ACHGT5_001413"
     CDS             complement(9722..12370)
                     /gene="mgtA"
                     /locus_tag="ACHGT5_001413"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN7217404.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12712..12886)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13059..13496
                     /gene="ndk"
                     /locus_tag="ACHGT5_001414"
     CDS             13059..13496
                     /gene="ndk"
                     /locus_tag="ACHGT5_001414"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN7217405.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13518..14384)
                     /locus_tag="ACHGT5_001415"
     CDS             complement(13518..14384)
                     /locus_tag="ACHGT5_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN7217406.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14544..16637)
                     /locus_tag="ACHGT5_001416"
     CDS             complement(14544..16637)
                     /locus_tag="ACHGT5_001416"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN7217407.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17386..18009)
                     /locus_tag="ACHGT5_001417"
     CDS             complement(17386..18009)
                     /locus_tag="ACHGT5_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217408.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18168..19565)
                     /locus_tag="ACHGT5_001418"
     CDS             complement(18168..19565)
                     /locus_tag="ACHGT5_001418"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN7217409.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19597..20568)
                     /locus_tag="ACHGT5_001419"
     CDS             complement(19597..20568)
                     /locus_tag="ACHGT5_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7217410.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20942..21367
                     /locus_tag="ACHGT5_001420"
     CDS             20942..21367
                     /locus_tag="ACHGT5_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217411.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6291 a         4504 c         3782 g         7243 t
ORIGIN      
        1 ttcgcctaaa agttttctga atagttgtaa ttgtctgaaa attttttatc attagtcatt
       61 gttaaatatt gctatttatt cttaatataa attttgattg tgaatttaaa agaaagaggt
      121 catttatgga agataaaaaa ttgaaaaaac aacttagctc cagacatatc accatgttag
      181 ctttaggtgg agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg attgggatcg
      241 ccggcccttc tgtattgatt gcttttttga ttggtgggct gattttatat atcgtgatga
      301 aaggtctggg aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta tctggtttga
      361 tacgccctta tctaggtgct cattctgctg attttactga ttgggtctac tggtctatgt
      421 ggattatcaa tatcatcgct gaagctgttg cagctgcttc ctttttgcag ttgtggttcc
      481 cgcacattcc agcctgggta tttgttttta tgttggctgt tttaactacg atcatcaatc
      541 tttattcggt acggttattc gcagaaacag aatattggct ggcttttgct aaaatcagtg
      601 tcattatctt actgatcatt tttgctactt atcttgtcgg tcagcaaatg ttgggcacag
      661 gcgttttccc tacccttcag caattgactg accatggtgg gtttactcct catggtatga
      721 agggaatcat cagttctttg cttgtagtta tctattctta cggcggttct gaactgattg
      781 ctatcacggt aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggtg
      841 tcatgggaag aatcatttcc ttttacatca ttcctttgtt tttgcttttg attctttttc
      901 cttggaatac tttagcaggc acgaatgtca gtccttttgt gatggtattt gaaaaaatga
      961 atatcccatt tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta
     1021 tcaattcggg tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag
     1081 gttcctctag aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgttttat
     1141 tttgtaccag tgttctttat cttggtgtag ctctttctta ttttgtcggt gacaaattat
     1201 ttggctatct ggcaggttcc ctttcgtata cagtattatt gatctggatc attatcagtg
     1261 cggcatcctt tgtactggca ctaagaaaag gaacattatg ggataaagga atcagctttt
     1321 ttgcattagc tgtcttagga ttgattttcc ttggtatcct gttcacgaac tccattgggg
     1381 taactgtatt gactggcctg ctttacctgt tcatttattt cagttatcaa aaaaagaatg
     1441 atgcgttcgt attgacgaat gaatgaaatc gatagaaaat tttattttat ttatgtggaa
     1501 tagatggagc tattgaagaa aggagttgtg acaaaagtct tgttgcaact cctttttccg
     1561 aataaacagt ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat
     1621 tgattgttct ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt
     1681 taaatgaaaa agtattggaa gtttttgttg atgatcggtg tttcaacggt agttatgttt
     1741 ggtatgatgt acttgaatgt ctatgcgctc gatcatatct tcttcagtcg tacacgagta
     1801 ttcatggcgt tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa
     1861 atgtatgaaa acaaaaagat aaatgttatg atcttagccg ctagtcttct tgtttttgcc
     1921 ggttccttgt ttatggtacg aagccaaact actgtcaacg acacagcttg gatgaaggcc
     1981 atgattcctc atcattctat cgctatcctg gcaagtaaac gagctgattt aaaagatcct
     2041 gaagtaaaaa aattagcgga agaaatcatt caggcacaag aagaagagat cgacaaaatg
     2101 aaaaaaatga tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt
     2161 ccagaagtta tcagcttcaa gaaataagaa gaagctactt ctggaacgcc gtgaataagg
     2221 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc
     2281 ctatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttatta
     2341 agaggttgta aaatcagcgt tctgacctga gtattagaga gacaaatgga aaaatagttc
     2401 ctcatatttt gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttatc
     2461 gagaggttgt gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt gaaatggctt
     2521 tccacatttt tgtataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttat
     2581 tcgtaaaagg gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct
     2641 aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa
     2701 cctgtgaagg cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc
     2761 aagtaagtga gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa
     2821 ggcactaagt agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga
     2881 tcaaatcctg ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg
     2941 aattgttcac caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa
     3001 tttggtgtgt tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat
     3061 tcgatcaaca atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata
     3121 ctttcgattc ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca
     3181 aaccctgatt cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa
     3241 atcattccta ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc
     3301 catgcacaca gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt
     3361 gcttctagaa catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt
     3421 cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata
     3481 tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg
     3541 gataatggag acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc
     3601 gctggattta agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata
     3661 acagtaaagg cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg
     3721 acaaaccact ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct
     3781 gcttgtatcc atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt
     3841 cctggtttga tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc
     3901 actgcataca acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt
     3961 aatccgacaa cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct
     4021 aaaaaacttg gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg
     4081 ttctaaaaaa gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta
     4141 taaaaataaa aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt
     4201 aaaaacagat ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa
     4261 ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa
     4321 ccgctactgc gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa
     4381 tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt cacacacaag
     4441 attatttttt actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt
     4501 tcttgcttga ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg
     4561 agtagttctg ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg
     4621 aattcttctg tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt
     4681 ggtatgactt ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt
     4741 tctatttctt catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa
     4801 gaaggagtaa cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg
     4861 cccgtactta tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat
     4921 gtttgccgat ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg
     4981 gcagctactc ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag
     5041 cgtagagcgt tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt
     5101 tcaggtgctg ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc
     5161 gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg
     5221 acatcctcaa aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct
     5281 tcttcccctg gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc
     5341 actatgctgg cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca
     5401 atacctggta aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa
     5461 atataacgtg catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt
     5521 tctttttcta attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca
     5581 ggatgttcgt ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga
     5641 tccttcaatt ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat
     5701 ttttcttatt ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc
     5761 tcgattactt tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta
     5821 ttgttttcat cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc
     5881 actggttcta agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg
     5941 ctattgatca ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat
     6001 tcagaaatgt ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct
     6061 tttgccttat ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca
     6121 tttgtaacat cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc
     6181 aaagtgttag aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca
     6241 gttccgtgtt ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca
     6301 atcgatccat ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc
     6361 aaattaatgt tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct
     6421 ccaataatcc ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg
     6481 ctacaccctg ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt
     6541 ttcatttttt ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa
     6601 ttgacttaaa aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg
     6661 gaatcgatta taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc
     6721 agccatggta gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc
     6781 aggcaaacct tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc
     6841 ttctgccgct tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc
     6901 aaaaaacgga acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt
     6961 tccaactaag aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga
     7021 acgaatgata ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc
     7081 attagggcgt gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa
     7141 tgctgtccaa aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat
     7201 caaataaaca ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga
     7261 tcactttgat acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt
     7321 tgatcaaata atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca
     7381 aactccctaa cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta
     7441 cttgatattt gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac
     7501 ttaattccac cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg
     7561 tttctaatgc ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt
     7621 caggattgct gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa
     7681 ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg
     7741 ttaaacctaa ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg
     7801 gatcaagtgc actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc
     7861 gtgcgattgc tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt
     7921 tttcttccaa accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg
     7981 attttccaga atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta
     8041 gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag
     8101 agagctgact cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca
     8161 aattcatcac acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa
     8221 catcgccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt
     8281 gtttgaaaca tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa
     8341 aagcgtcctc aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca
     8401 cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa
     8461 gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc
     8521 attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct
     8581 tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca
     8641 taaactatgt cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt
     8701 ccctcaagcc caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc
     8761 cgaatttgcc tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg
     8821 actcttctca ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg
     8881 aaatcgatat tttgttgttg ggattaatct acatgaggaa aactgaaaag tcaagtacga
     8941 aattcaaatt attttttcta tatttatttt tctgtttttt tatttaacat ttcaataaca
     9001 gttcgaagat cttttccttc cgcaagaagt ttcaattttt ccttttcttc gacggtataa
     9061 tttcggacaa aactggaatc aggtatcaaa acaacgccta atcctgctcg tgaagcagct
     9121 gttgctccta ctaaagaatc ttccactgca aaggctgaac tagcttctaa tcctgttcgt
     9181 tccaaagctg tcagataagg aatcggtgaa ggcttgttct ccgacacttc gtctccgaat
     9241 gtaaacgtgt ccaagtaatc agctaattta aagtgatcca agatatctgt tgctcttttc
     9301 gtaacagtgg tcgtagcaat gcctagtttc atatgacgtt cttttaagtc tgccaatatt
     9361 tcttttgcat atggttttaa gtggatggct ccgcttgtca gctgttgata aaaatattct
     9421 tcacgcttcg ctcggacttc ctgtactttt tcagggctac ctgctgcttt cactaaaata
     9481 tcaaaacttt gtttccagtt ttttccgccc caagcgtcaa cgatttcttt tgggatatgg
     9541 atatcaaatt gctgaaatcc ccaaagccag ccatcacgat acgttttttc tgtgtcaact
     9601 aacaaaccat ctaaatcaaa tattgcacct ttcatctata cattaatcct ttccaaaaca
     9661 atacacatag tttagcacga ctggtccaaa aagaacagaa gcaaagaacc agccttctcg
     9721 tttataagag ttcttggaat cgacggatat agattttctt cacagctgtc acaagcgcca
     9781 gataagcgat gactgtagca aataaccata accagtaatt gccaggaagt ggcatcaagc
     9841 ctaattcagc accaaaactt gtaaaaggaa ggacagaacc gacaataatt cctaatgtag
     9901 tgataacaaa catggcaaat gttgcgttgc tttggataaa tggtactttc ggtgtacgca
     9961 gtgcatggag cactaaggtt tgtgtccata gtgattcgac aaaccatcct gcatggaata
    10021 atgcgatgaa aatcactcgc tgtgaactat ccaatgtatg gaaacttccg cccactacag
    10081 caggacaaat aacaaaatac atcaacaagt aagtagtaat atcaaatatc gaacttgttg
    10141 gaccaaagta aaccatgaat tccccaatac tagatgcatt ccattttttt ggctcgtcca
    10201 aatattcttt atccatccga tcccatggca aggaaatgca ggaaacatca tagatcagat
    10261 tcaaaaacaa aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta cttgcaatca
    10321 gcacggaaaa catattgcca aaattagaac ttgctgtagc cttgatatat ttcatgatat
    10381 tcccaaaagt ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta
    10441 aaaggatcac gtctgcagat tctttcgcaa tatccacagc tgtatctaca gaaataccga
    10501 tatcagccac tttcattgca ggagcgtcat tgataccatc tcctaaaaat ccgacagtat
    10561 gaccattttt acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc aattttacga
    10621 atacttcatg gtcttcagct acttgtgcta attcatgatc actcatttcc gagattttct
    10681 caccggtaac caattcattg atctctagac ctacttgacg gcacactgaa cgtgtaacta
    10741 attcgttgtc cccagtcaga acttttacag ctacaccatg ttctttcaga gctttcaacg
    10801 cttgtttcgt tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt
    10861 ctgactcatc tttgactgaa aattctccaa cgacgcttgg attggtcttt tgtgcgacac
    10921 caatcactcg taagccatct tcattaagtg actgtacttt tgcaagtacg gcttcttttc
    10981 gttcttttgt taatggtgta acagtaccgc caagatcaat aaatttagaa atgttcagca
    11041 tttcttcaat cgcacctttt gtgatcattt gtgttttacc agctggatca gcaaccacaa
    11101 cactcatacg acgacgttca aaatcaaaag gaatttcatc cactttttga taattgatct
    11161 tatctgtatc taaagtttct gttgcttcat caatgatagc cacatctaac aaatttttca
    11221 gccctgtttg atagtagcta tttagatacg catggcgcaa aacacgatca tcttcatttc
    11281 cttcacaatc catatgatat tcaagaatga ttttatcttg tgtcaatgtc cccgttttat
    11341 ccgtacataa cacatcgatt gcaccaaagt tctggatggc attcaaattt ttgatcactg
    11401 ttcctttttt ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa
    11461 gcatttcagg tgttagacca actgctacag atagtccaaa aagaaaagct tctagccaat
    11521 ccccttttgt aagcccattg atgacgataa ctgctggagc catcaatgac ataaatttga
    11581 tcagtagcat ggatgtttta tggatgccta attcaaagct tgattgaatt ggtttttcag
    11641 atagcgtttc tgctacatga ccaaatagtg tttcatttcc aacagaaaca atgattcctt
    11701 ccgcacttcc actgatgaca ttacttccca taaagacaag attttcgtaa cttgtttcgc
    11761 tttgctgatc gtcttcacat actttcgcat gcttttctac tggatagctt tcccctgtca
    11821 atgctgcttg ggaaatgaat aaatcctttg cggacaataa acgcatatcc gctggtatca
    11881 tatcccctgc tgaaagccga acaagatctc cacagacgat ttcttccata ggaatctctg
    11941 tgtactttcc attacgtttt acagctgccg ttactttcac aagtgatttc agtttttcag
    12001 ccgcttgatt ggaacgtacg gactgaatca acgtcattgt cccgctgacg ataaccatag
    12061 tgaagataat ggctacaccg aaaagatctt tatctccagg agctgctaat acatattccg
    12121 tgatgaatga aataattcct aatgcgatca acactaatgt aaatggcgtg atgtatgctt
    12181 tcaatatttc tacaatgaat ggtgtttttt tgccatatga aatcgcattt tcaccgtaat
    12241 tttctcttga acgttcgatt tgttcttctg tcaatccatt tgctgatgca tggaatagtt
    12301 caaataattc ttgtttcgtt tttccagcaa aagctgcata gattttttgt gtatctttct
    12361 ttttttccat ctcaatctct cctttgtgtc caaaagacag ctgttctcgc tgcttttggt
    12421 ctttagctat tcatcggatc gatagagaaa aaactgaaag gggctgagaa cagatagata
    12481 atagccctgg cctttcctct agatccttgt ttttcgaatg actgtcatcc atttgttctc
    12541 atctccttta ctaagtaatt ttaattcatg acttccaatc atttttcgaa tagttatttt
    12601 tccaattaga ccacctcctt tttatttttc accgatgggg atttgcggca acaaaaaaac
    12661 ctctcgccaa atactcagcg aaaggtttga aaaagcatat taaaaaaagc ttctcagatc
    12721 tcatcgttga gttttgacac tgtacaacgt aaaaagcaga tacctgcttt tagctacctt
    12781 atgaaaagcc ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg
    12841 gcagtagcct atgtttgtat aggagcctca cctaacaaat cttattcggt tttccttcgt
    12901 taatataaca actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt
    12961 taaaatacac acgttatatt cctgtctcac gcggttttat ttgtctcatt ttccacctcc
    13021 cgctataata gaaatatagt aggaaacgag gtgctgttat ggaaagaaca cttgttatta
    13081 tcaagccaga cggcgtgaga cgccatttag tcggaagcat catccaacgt tttgaagcaa
    13141 agggactggc cattgctgaa atgaagtttg aaactatgac tcctgaatta gcaaaagaac
    13201 attatcagca cttgactgag cgcagttttt ttgatgaact gatcgattac atgacttccg
    13261 gtcctgtcgt ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg
    13321 ttggagcaac aaaagctgcc gatgctgttc caggtacgat ccgaggagac tacgcgctgc
    13381 cggggactga aaacatcatt cacgcttctg attcccgtga tgctgcggta aaagagatcg
    13441 cccgcttttt cccgccattc gatataaaca aaccgtctga aaaagcggcg aagtgataca
    13501 aattctaaac tgctggtcta gttgctgggt tcagcaatgg ccagcagttt tttctctaag
    13561 gacctccgtt gattctttcc taacatatat tcgagcactg tgaagaattc ttggtagtct
    13621 ttcgtctttt cttcccctaa agcagcagaa atcaatcttt gataataatc tgccatcaat
    13681 gtttcataga tcactgtact tctttcttta tgtgctaagc tccgtttcat atcttcattc
    13741 acactacgtg cctttttcaa ccgaccattt tctatataaa acgaagtcag gttcattaaa
    13801 aaagtaaaga gatactgctc atgattgttc aactgtgcta atcgtcgcat tctttttttg
    13861 attttcaaat aattgttctc aatgaaccga tcactgaata tccaaggaaa atcagctaga
    13921 atagatagtt caaattgacc atagtctggt atctgcatca agtattcttc aatgattcgg
    13981 cattcataac gtaaaaattc aaattctgtc tcagatactt ctgttttttc gaccatgtga
    14041 agattcaaaa ccaaataata cagatcttct gttttcttaa aatgcctacg gttattgctg
    14101 atctctttat aaggatcaaa gctgtcttct tgtacccgat ctagcattcg tctttttttc
    14161 tttaacttta ttggttcatc cagctggttt cgcatccatt catattctgc aagtgtcatg
    14221 ttcattcgct ctaaaaatga tctcaatgtt acaaatgaca tttttttcac tgaatttgtc
    14281 tcaatcccga ctagagaagt cttaaaacag attccttccg ctaattggga ctgtgtcaac
    14341 ttcttgttga ctcgaagttt cttgatcaat tcaccatcat acataatagt tctccttttt
    14401 aatttaaatt tttccacatt caataaatag atacgttaaa aaggaaaaat acacgaaaaa
    14461 aataaaaaaa tgtttttttc ataaaaaata ccaaccttca agcattagaa tgttgtctaa
    14521 ctactgaagg ttggtttgat tgtttattga ttattttttt gctgttcaac gtctgttctt
    14581 gttactggat ataagttggt tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa
    14641 tgttcgcctt tttgcttttc gattgcttca ttacgttccg cttcattttt atagtcatat
    14701 tttttcggat caatcgtctc aaatccgttt ggcacataaa accgcaagag attttcctga
    14761 ttcagcttat ctgaaagact taacgcagtc ttgacttttt cctgatcttt ggagatttcc
    14821 tcatcgattc ctgctgcttt cgtatcgatg atttctcctg tctgattatt gtaagctttt
    14881 cctcctagga cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc aactacttga
    14941 tcatgctgag aagataataa gtctgtacca aaatgaagat aatctttatc atcgattccc
    15001 aatagatgta gtaatgtcgg caaaacatcg atttctccac cgtattcatg attgactgtc
    15061 cctttagtat aaccaggcat atggatcatc aatggcactc gttgcatctg tgcgttgtca
    15121 aattcatccc atgtatctgg atctttacca agagcgcttg ctaaatcttt attatctgta
    15181 ttcgagatcc caaaatgatc cccgtaaatc acaaagattg aattttggta aataccagat
    15241 tttttcaaat acgtaaaaaa ttgttccaat gattgatcca gataatgtgc cgttcgaaca
    15301 taattatcta ctgtgctatt gccagtattc aaagatggaa agtcgaaatt cttgtcatcc
    15361 gtataataag gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga
    15421 tgctcaagat attgggcaga ttctctgaat aaatctttat ctaatatccc ataacctaac
    15481 gtttcgtcgg attcatcaaa ataagatcga tcaaagaaat tgtcataacc taagttttta
    15541 tatacatgat ctctattcca aaaactagct acattcccat ggaaaacagc gcttgtatag
    15601 ccttgctttt gtccaaggat cgctggtgcg gcttgaaaga cattgtctga cccaagttct
    15661 gtgaataatg agccttgagg taaaccaaat gtgccagttt ccaacatatt ttcagcatca
    15721 cttgtctttc cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc
    15781 tggaaaatac tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat attaatcaaa
    15841 aactgctgga aactttctaa atggagaacg atcacatttt tccctttagc gatcccaaat
    15901 gtttcgggat ttggctctgc ataatgtttc tttgtataat ctaaaacttc atcaatccca
    15961 ttactgcttg catgcgcgcg aacactgcta gtcattcctg ttttgattcc gtcataaata
    16021 gtatacgcat cgattcctag atatttgaca atatatgaac ggtcaaatgt tcgctgcagc
    16081 aattgtggac ggttggcttc gctcagcgct agatttcctg aaaaaataag ccctgaaaga
    16141 caggtaacag cgatagccat cggtttgccg acagggttgc ttttgatagg tattttcttg
    16201 accttcaacc aaaccagtag cccaatgaac acagcgatat ccagccagta gatgacatca
    16261 tggggcttca tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt cgaaaagcct
    16321 aatacagatt tgactgtaat gaaatctgtg aattcccgat aaaaaataat attcaagtac
    16381 aacaaaagcg tatttaaaat atcaatgatc aaaagaatag ctaacgctgg ctttggcttt
    16441 ttaatataca aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt gatccacaag
    16501 ataaagtact ggatcgtacc ttccacacct aatgaaaaat ctgcatagta ggcaatgatc
    16561 gtcttcagcc agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt ttggcacaat
    16621 tttttaagtt gtgtcacgta tatcacttct ctctattcac attttttctt attttgctcc
    16681 tgaaaaatga aaatttccta aaaaagttgt tatcttaaga tttttttatt ctttctaaag
    16741 attattccgt ccacttttca cagcataaaa ccgctgcgat gtaagcgatt aaactaatga
    16801 agagataaaa gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc agtttctcgg
    16861 tattcgtaca aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta tttcttgatg
    16921 caggacagct ttttcacaac ttcttcctta aacggtgttc tcctgccaac tcctcggcca
    16981 caagtcacaa tatttataaa acatgagaag ctgttttttg aaattgttcc ttgtggctca
    17041 gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccctcggt
    17101 attagattat gtcaggacgc taattttaca acctcttggt aaacggtgtt caaaagcttg
    17161 catctgcggt aataagccca accaaacgaa aaatataagg agctattttt tgaaattgct
    17221 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    17281 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt
    17341 tctctaatac tcaggtcaaa acgctgattt cacaacctct gtttttcatt gtccatagga
    17401 ttgatattgt ccttgtgatg actcattcgt aataattgag gcttgatttt cttcacttcc
    17461 cgttatcgtt tgtccactgc cagaatctga aggacttgtt cctgtcgact cgtttttttg
    17521 agaagagaca gttgttttct tgacttgttc atttgtcttt ttccattccg ctaaacgatc
    17581 ggcgaccact tttttgtcta ctttttcaga ataagcggtg atcacttttc catttttcaa
    17641 acgggctact ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa
    17701 ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc
    17761 ttcttttcct tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt
    17821 atactccgcc caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct
    17881 tgctacgatt ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc
    17941 tgcggataaa ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt
    18001 tttcctcatg ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat
    18061 gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa
    18121 atggtaagaa tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat
    18181 ccgtttttat ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca
    18241 tcttgtgcta agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac
    18301 cagttatcca tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct
    18361 tgatggacat atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg
    18421 ccatcttctt tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag
    18481 cagtcgatat ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca
    18541 tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa
    18601 taatcctcag gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg
    18661 cgtggctggt aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa
    18721 tcctctgcat ggatcacagg catgagatca agttctttta ctatctcaat caattcactt
    18781 ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt
    18841 tgcgctgctt taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc
    18901 aagatgatac caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct
    18961 gaagccaacg cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga
    19021 taatgccatc gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac
    19081 cattttttta ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct
    19141 tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa
    19201 tgatacagat tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga
    19261 tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat
    19321 tggatagatg ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga
    19381 tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt
    19441 tcttttttgt accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct
    19501 tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt
    19561 ttcatcaaag cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt
    19621 ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg
    19681 ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat
    19741 actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct
    19801 gacaccttct tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa
    19861 aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga
    19921 atagtctcct gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc
    19981 tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt
    20041 tttatgtcct tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac
    20101 taatattgta tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact
    20161 catttttgtg agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc
    20221 atcgatcaca tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc
    20281 aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga tccctgagac
    20341 aacttccgac ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg
    20401 attattcttc aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc
    20461 tgctattttt ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt
    20521 tcccactgaa acaccagcca tcttagccac atcacggatc gatgccatac attctcactc
    20581 ctgccagtta catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc
    20641 atagtgttgc ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct
    20701 cactattaat tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt
    20761 taaaagattg caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt
    20821 aaaattttat tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc
    20881 cgtttgaagt tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat
    20941 tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg
    21001 tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa
    21061 agcggaaaaa gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga
    21121 tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca
    21181 gacacaagga actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa
    21241 gatcactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac
    21301 agtcatattt acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag
    21361 tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa
    21421 gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat
    21481 gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg
    21541 acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa
    21601 attatgtaaa aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt
    21661 tatgaacttc aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg
    21721 atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa
    21781 aataaccaaa cgatagaaag gcttattcta catcacgtag
//
LOCUS       DAZBWR010000044        21673 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000044 DAZBWR010000000
VERSION     DAZBWR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.044"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..2695
                     /locus_tag="ACHGT5_001421"
     CDS             20..2695
                     /locus_tag="ACHGT5_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN7217412.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2877..3116
                     /locus_tag="ACHGT5_001422"
     CDS             2877..3116
                     /locus_tag="ACHGT5_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217413.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3132..4505
                     /locus_tag="ACHGT5_001423"
     CDS             3132..4505
                     /locus_tag="ACHGT5_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7217414.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4509..6146
                     /locus_tag="ACHGT5_001424"
     CDS             4509..6146
                     /locus_tag="ACHGT5_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7217415.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6309..7241
                     /locus_tag="ACHGT5_001425"
     CDS             6309..7241
                     /locus_tag="ACHGT5_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217416.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            7357..7677
                     /locus_tag="ACHGT5_001426"
                     /pseudo
     CDS             7357..7677
                     /locus_tag="ACHGT5_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            7855..8214
                     /locus_tag="ACHGT5_001427"
                     /pseudo
     CDS             7855..8214
                     /locus_tag="ACHGT5_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            8278..10935
                     /locus_tag="ACHGT5_001428"
     CDS             8278..10935
                     /locus_tag="ACHGT5_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN7217417.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            11007..11483
                     /locus_tag="ACHGT5_001429"
     CDS             11007..11483
                     /locus_tag="ACHGT5_001429"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7217418.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            11497..12285
                     /locus_tag="ACHGT5_001430"
     CDS             11497..12285
                     /locus_tag="ACHGT5_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN7217419.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            12287..13084
                     /locus_tag="ACHGT5_001431"
     CDS             12287..13084
                     /locus_tag="ACHGT5_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN7217420.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            13103..13498
                     /locus_tag="ACHGT5_001432"
     CDS             13103..13498
                     /locus_tag="ACHGT5_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7217421.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            13500..14621
                     /locus_tag="ACHGT5_001433"
     CDS             13500..14621
                     /locus_tag="ACHGT5_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN7217422.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            14641..15510
                     /gene="scrK"
                     /locus_tag="ACHGT5_001434"
     CDS             14641..15510
                     /gene="scrK"
                     /locus_tag="ACHGT5_001434"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN7217423.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            15711..16220
                     /locus_tag="ACHGT5_001435"
     CDS             15711..16220
                     /locus_tag="ACHGT5_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFN7217424.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16320..16970
                     /locus_tag="ACHGT5_001436"
     CDS             16320..16970
                     /locus_tag="ACHGT5_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217425.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17421..18359
                     /locus_tag="ACHGT5_001437"
     CDS             17421..18359
                     /locus_tag="ACHGT5_001437"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN7217426.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18372..19424
                     /locus_tag="ACHGT5_001438"
     CDS             18372..19424
                     /locus_tag="ACHGT5_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN7217427.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19690..20325
                     /locus_tag="ACHGT5_001439"
     CDS             19690..20325
                     /locus_tag="ACHGT5_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN7217428.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            20516..21307
                     /gene="msrA"
                     /locus_tag="ACHGT5_001440"
     CDS             20516..21307
                     /gene="msrA"
                     /locus_tag="ACHGT5_001440"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN7217429.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7366 a         3232 c         4416 g         6659 t
ORIGIN      
        1 agaaagtgag tggtaattaa tgaaaatgaa tcacacttca attcagaagc atttacaact
       61 gttaaataca ctggggattt cttatgttaa tttgagaact ttttctacat taaacaagaa
      121 aaccgtttct catatttttt ctactgcaga ttctcaagca attattaact atttagaaaa
      181 acagaaattg tctgaaggta cagtaaacat aacttataat cctattaaac aagaggtgat
      241 tacacaaaaa aagcattctg tgagagacaa tgatattgaa aaaattcggt ttgtatttat
      301 tgatattgac cctaaaagga aagagggaag tgcaacagat agcgaaaaga aaaaggcaga
      361 aaatgttatg gaacaagtag aacgttattt gaaagaaaag agaatagaat catttgtaaa
      421 agttgatagt gggaatggct accatatttt tcttcccata aatgaacaac caaataatca
      481 cgagacgatt cttactattc agaattttct tcaattactt caccgaagat ttggagtaga
      541 agatgaagtt gatattgata cttctgttta taatccttca agattatgca aattagttgg
      601 tacaccagcg gtcaaaggaa agtccacaaa agagcgtccg catcgtttat ctagtttcct
      661 ttctattccc gataacatag atagaagtaa ttgttttaaa attcttgaac agatagttga
      721 agaaaattcc atgatgtcaa cctctagagt aaaaagccat ccatctttta gagaagtgtt
      781 accttttatt caagcagact ataaacaatg gttagctcac tatcctgaat taacttatac
      841 cataaaagag ggcgatcata aaggagttac cttacttata tttgacgaat gtccattaag
      901 aaaacacacg aataattcta atggttcttg tttgtctgtt acggctgata ataagattcg
      961 atttcattgt ctgcatgctt cggataaaaa taaagatatt caagactttg taaaaaaata
     1021 tccgttgcca aaacctgtaa atccttctgt agaatcatta gaatccaatc aagaatatta
     1081 ttttgaccag tttaaactga atcaagaagg agtatttttg gtgaaagaag gtcaagaaga
     1141 aaaagttgct tgtccgatgt ttattaagca aatacgcaaa aatatcgaac aaaaagtcat
     1201 tacacttgtt cttgcctatt atgtcgatca ttgttgggat gaaatagaaa tagaaggtgg
     1261 atgtttacaa actaataatt ttaaacaatt agcaaaacat ggcttaatca ttaaagctag
     1321 aagagaagct gaagtcgttg actttttaca gattcaacgg gagaatgctc caatcttttt
     1381 tgaacatgaa tctcttggct ggaaaaaaga aaatgaagca atgatttttc agttacaaga
     1441 ttcttttgga cgtgaaattc cttctgtatt aactaaaaac tcatgctacc aattaacaac
     1501 taatggtaca gaagcggaat ttgacgcatt ggttcataag tatgttttag gaacgaactt
     1561 agaacttgct tggtcgattg gtattgcttc tattgtgtta ggctatttaa acgttacgaa
     1621 ggctaatact tttttgagtt tactaattaa cctacagggc aagagcacaa cgggtaaaac
     1681 cactacgtta cactttattg cctctttatt tggaaatcca actacggttc ttagaaatat
     1741 gaatgctacc aataaagcca ttataaagct agcatcaaac aatcagggag gtgttccact
     1801 aatactagat gagttaggag cagcaactca aaataacttt agctcgttga tctatcaatt
     1861 agcttcagga gaagaaaggc taagattaga ttctaattgt cagcttatgc cacaagagac
     1921 tttttctgtt ttgaccctta cctcttctga agagcgtttg gaaaattatt tcaatgagaa
     1981 attaactggt ttgaaagttc gatatatcga attttctgat atcaagttta caaaggatgc
     2041 gaatcacgca gagaaagtta aaagtctttc ttctaatact tttggttggg gtataaagtt
     2101 atttcttttg aaactctttg aacaagggat tgcaaaaatt gaagcaactt ttgtcgaagc
     2161 tcaaaaaaaa atatgggctg ttttacctga acattctttg aagcaacgca ttggtaataa
     2221 tctagctttg atttatagtg gagctctcct aattcaagat atactgggct atcctttacg
     2281 attagagcat atacagacgg tgcttattaa ttcgtacgaa cagttaattg tagatactag
     2341 tttaggaagt gagcaaacgg tatttgaccg agtgaaaacg ttgttggtga tgaatagcca
     2401 taaattttta acaaacagtg acaacaaaca tacgcccagt acgatttggg gaaaagtatc
     2461 aattcaaaaa aatggggata ttaaggtaaa tatttttcct atcccgtttg aggacttgtt
     2521 aaggcgagaa tttcaagtaa aggatctaac ctatcttttt agacaattgc ttgattcggg
     2581 taatatgtgt gcagaaaaag ggagaagaac gaaacgtatt cacatgaatc gtcaagttct
     2641 tccaacatac gagattctat taccttcttc actcaaatcg tattttcgat tgtaatttag
     2701 aataaaccaa agtatgaaag tgaggcacca tgttatggaa atatttctta ttgatttagc
     2761 aaaacaactg attacttatc tgtatgatga cgagcaaacc caaacggaag taaatgaagc
     2821 ttgatctatg cttcatttac ttcattcaat caaacattta agaaagggga attctaatga
     2881 ctaaaaataa aatgacacta aaagcagagg ttcttctgta tattcaagaa catttttcta
     2941 atcaagcctt ttttacaaaa ccaatttact tggctttcga aataagaggg gtatctgctg
     3001 gatctattgg aggtactctt caagcgttga agaacgaagg atatttagaa aatcactttg
     3061 tgcaacgttc gttcaatgga agagtcgcga aagaatggta tttagttcat tcatagagag
     3121 gtgattcaag catgcgtgtg gcttgttatg atcgaacgtc tgtttatgaa gaagcgaaaa
     3181 tcagtaatga cgctcaatat gatgcagtga aagagtatgt tttagagaaa tatgaacaaa
     3241 ttcctcaacg attttcagac aaaggagaat caggtggaaa agcggatcgt ccagcttttc
     3301 gacagcttag agcttgggtt tgtgcgtcag aagaagaggg ggtgatctat gtctggcgtt
     3361 atgatcgatt ggcacgtcaa gccaaaatag ccttagaatt tattgagttg tgccaacaac
     3421 atcaggttca agtttattcg atttctgaac ctcttcctgc ttctttctcg cacgcgtcaa
     3481 cgaataaact atttgtgcaa ttattgttca cttttgcaga acaacaacga aacgtcatta
     3541 ttgaaaatgt tcgtgccgga cttgcctata aaaagcaaca acggcaatat ttgtcttctg
     3601 cggttccata tgggtatcgt ttagtgaatg ggaaaatcca acaagaagca catgaagcaa
     3661 aagttgtccg ccatatcttt caactttatt tgactaaaaa atatggctac aaaaagcttt
     3721 gtcaacgatt gacgcaacaa aagttcttct tccgtgaacg tccttttcag ccctatcata
     3781 tttatagtat cctaaaaaat ccattgtact atggcgaaat caaaggagga tctttgggga
     3841 agtatctagg aacctttgaa ccgattctct cgaaaacaat ctttttacaa gcccaagaga
     3901 ttcgccaatc aaggtgtaca gctaaaaaag atacgtatcc gtacttatta cgtcaaaaaa
     3961 ttcggtgtcc cttctgtggt cgtcatctga gttcgaagta tcaatggaat acaaagaaga
     4021 cgaagacgct tcattattat cattgtaccg accgaagctg ccaaggaatc tttcttcggg
     4081 cagaaagggt tgagtcagaa gtattggaaa agatacaggc tttttttcaa tacgaaggcg
     4141 tctatcaagc cttgattgcc caattgacgc aacaattagc cgaaaagcga aaagatgaaa
     4201 aacacaaagt aatccaacaa aagaaacaag aagcacaatg gctcaaggca tttgaagagg
     4261 gcaattgttc actggaagaa ctcaagcaac agctgcaagc tcttcaaaag ccgaggtcta
     4321 cttcttcgtt tctttcactc caacagtgtg aagcaaaagt gaaagaatgg cttcaactaa
     4381 aagacctctc tagtcgacaa ttgttatgtg accatgtttg tcggatcgag gtaacaaagg
     4441 aaaaaacagt ctgtgggatt tacttccgac aaatgacaca aaatatttta gaagggaacg
     4501 aatgatctat ggaaacgaca ccaaccaaac gagtcgcatt gtatattcgg gtgagtaccc
     4561 aagaacaagc tttagaagga catagtattc aagcacaaga acaaaatgga cgacagtttg
     4621 ccgaacggat gagctatgaa gtggtagaag tttatgccga tgaagggatt agtgggaaat
     4681 ccacaaaaaa tcggttcgcc tatcaacgaa tgatgaacga tgctcggaac catcgttttg
     4741 atctactagt gatttggaaa ttgacccgtc tcggacgcaa tatgttagat gtcttacaaa
     4801 ccgtcgagga actgttgcga tatgatgtgg ggttacattc gatctcggaa caatttgacg
     4861 tgactacttc gacaggaaaa ctaatgttac aactccttgg gagtttcggt gaatttgaac
     4921 gcaaccaaat tgcggagaat gttcagatgt caatgcaaag cttagtgaaa gataaaaagc
     4981 gttatgcagg tggacgacgt ttaggctatg tcagtggtat tgacacgaat ggtcaaaaac
     5041 aattggtcat tgaaccagaa gaggcgaaaa ttgttcgtct gatctatgca aaatatttag
     5101 gcggagcagg gtatcgagcg attgccaatt ttttgaatcg tcaaggatat caaacagtca
     5161 aaaagaatgc tttttctacc acggcagtca aagatatctt acgcaataaa atttatgcag
     5221 ggtatttaga atatgcacgc tatatcaatt gggaaaccaa acgacgaaag ggcaagaacc
     5281 caaaaccgat tttagtgaaa ggtacccatg aaccgattat ttcggaagaa gattatcaaa
     5341 aagtgcaaga acgccttgtt ttagaaggca aacagcccaa atggaaccat acaggtgaaa
     5401 atgtattgac aggcttgtta cgttgtccag aatgtggggc accgatggca gcgagtaatg
     5461 tcacaaatac tctaaaggat ggcacaaaga aacgaattcg gtattattct tgttcagtct
     5521 ttcggaataa aggagcaagt gtgtgtcatg ccaattcgat tcgtgccgaa caagcggaaa
     5581 catttgtcgc cgaccgatta aaggagatcg ttcagctacc agaagttctt ccccgattgg
     5641 tggcagcgtt aaatgaagaa atagtgcgac aaagccaacc attggaacaa gaactcgtgg
     5701 tgttactgga acgaaaggaa gaattgaaga ctaaaattga aaaatgggaa gctgctttgg
     5761 aagatagtcc tgaattgttt ccgatgttaa aagaccgctt agacgaattg actgaaaaac
     5821 gacgacagtt gcacattcga gaaaatgaaa tcttagggat ctttcaacaa caaggagagc
     5881 cgattcaagt caaagatgtc caacggatat tgactagttt ggatcgtttt cttgcccaga
     5941 gtgagaagaa gcaagtcaaa gccttgtatc gtacctttat tgagaaaata acctttgatc
     6001 ctaactataa ggagatattg gagattacaa tgaaattcac gcctgccgtg gtacaacaat
     6061 tgaatgagca atatcaaata gccgtatcca aaacaaagga tacggctatt tttgtgttaa
     6121 agacaccctt tacgttaacg atctagtcta ggttatatca aacaacgtta gaggggcgct
     6181 aacgcttctc tagcgatttg aaatcgttag atgagtcgtt agggaggtcc ttatcataaa
     6241 tagggatatt tattaatgaa tatttgaaat ataattgtat ataagaatta aaaagagtgg
     6301 ggatagatat gaaaaagatg ggtaagtgta ttttatgtga aaaatacact gaacttacta
     6361 aagaacatgt accacctaaa aaatcaggga atactggagg taaaaagacc agaactggta
     6421 atttagatga tttccttaag agcgatttta caaaaggtga ttttccgaag gggattaaaa
     6481 ggaaacctca aggaaatgtt tattatacgt tatgtagtaa atgtaatagt ttttttggta
     6541 gtgagtatgt tgaggaatat attagatttg cagaagacaa taaaaatttc ctttataaca
     6601 atacaagttt aaagaatggt aagtctgatt taacacattc aataaagaaa atgaattcac
     6661 ttagagttgc aaaagcaatt gtagcaatgt tttttagttt aaatggggat gaagattcaa
     6721 tggataagcc attcttagat tcggtacgag aatatcttag caatcctaaa agtactttat
     6781 ttcctcatga agactacaaa attataatga attattatct tccaataggt gctccacaga
     6841 attctaaaat caaaattcct aatgacattg taaatgatgg tgacactcac aatgtaataa
     6901 aaggttttat gccccttgat ctatcaactg ggagaaaaat tcgattttca gaaattcagt
     6961 atgaaggaat tggttttact cttatagatt taaaaaattc aacttttgaa ataaaagaag
     7021 gattagattt aaaaaatctc ctttgtgcag atgataaaat caaaaaaata caattgtaca
     7081 aagtccctat attaacacca acagtactta caatgttgga gatggaaaat aatattaatt
     7141 atattgactc aggttcagat ttgctgatta ggtggcaggt aataactaat tttcttatgg
     7201 aattaaaaaa atacagtaat actcaaacga agttctcttg aagttataaa gaatacaata
     7261 aatataaatt tcctttagac tatattaaaa tcagcaatcg aaggaaaata aacgaagaaa
     7321 atggtacgca aaatctaact attagagcaa aaacaggaag ctgctttgga agatagtccc
     7381 gaattgtttc cgatgttaaa agatcgttta aatgaattga ctgaaaaacg aagacagtta
     7441 caaattcgag agaatgaaat cttagggatc tttcaacaac aaggggaacc gattcaagtc
     7501 aaagatgtcc aacagatatt gactagtttg gattgctttc ttatccagag tgagaagaag
     7561 caagttaaag ctttgtatca cacctttatt gagaaaatca cctttgatcc taatcagaag
     7621 tagatattgc cgatcccgat gagattcaca cctactgtga tttgataatt gaatgagtaa
     7681 tatcaaatag ccgtatccaa accaaaggat atggctattt ttgtgttagg gatatacgtc
     7741 acgtagcttg ttatcaagaa taagtttctt tatttttttg ctttttctaa aactgagtat
     7801 gctaaaataa gagggtatat agcaacttta aataagagat aggagtgtat ctttttgggt
     7861 gcagaattaa atcagaagtt atttagtgcg gcagataatc tccgtagtaa aatggatgcg
     7921 tcagaatata aaaattattt attggggctg attttctata aatatttatc cgaccgtttg
     7981 ttggaacaag tggttctttt agcagatgaa tccttagagg aatatgacac agtaagcaaa
     8041 caaacgatgt tgtatcgtga attactttca gatgaagaat caaaagaaga tctgattgca
     8101 acaatcgttg atattttggg gtatgcgatt tcgccagagt atcttttcaa cgtcttagcg
     8161 gatcaagcaa aacaagcaac gttccaattg aatgatctaa acaaagcctt tttagcaagt
     8221 catatttatg gaaaaatact gtcggcaaaa ttcgaatagt gtcgaggagg tgctgtgatg
     8281 gaagaattaa aagaacgcat tgtcaattat gtcatagaga actctgagat taaaaataaa
     8341 tgggggaaca atacagcaca gaaaatatct gaaaaattaa atgttagtcg taatattgtc
     8401 tctcaatatt tgaatgaatt cgtaaaagat ggagaatttg gaaaaatcaa tactcgtcca
     8461 gtaatcttct tttctttacc ggagaaagtt ccaaatcaac aaatttttag ctcttcagag
     8521 gaagcggaaa aatggtatga tagctctttt agggttggaa tagaaagggt tatcggaagt
     8581 caaggaagtc tgcgaaatgt aattgctcaa attaaagcag caatctacta tccgccaaat
     8641 ggcttaccca ttttacttca tggcgagact ggaactggaa aaagctattt agcaaattat
     8701 gtgtataagg atttgttggc caataattat atagaagaga aggcatcttt catttcagtt
     8761 aattgtagtg aatatgccaa taatccagaa cttttcttgt ccaatttttt tgggcatgta
     8821 aaaggtgcat atacaggagc cgaagatgga agaaagggtt tagcagaatt agctaataat
     8881 ggagtcttat ttttagatga aattcatgca ttgagcaatg aatgtcagga gaaacttttt
     8941 cagtttatgg atacgaggaa atttcatcgg atgggtgata atgaaaactg gtacacatca
     9001 agttgctgga ttatatttgc aacatcagaa ccacctgagg ataaactatt atcaactttg
     9061 ttgagaagaa tacctgtaaa gctaacactt ccaacattag acagtcgttc tctttatgaa
     9121 agaaaacagt taatcaagat attttttaaa tcagaagaag aaagactagc aaagaaaata
     9181 ctgatatctc aacaattatt agatttttta ttaaactatc gctttagtga aaatgttggc
     9241 aatttgaaaa atatcattca attgacctgt gccaatgcct tttatttctt agatgatttg
     9301 gaagaacttt atattcagat gtatcattta ccacaatggg tgaatttcaa cggcatgaag
     9361 gttatgaaaa gacagaatga attaaaatat attcaaatgg atgatttgga aaaagtagac
     9421 gaagttttta atatcaacga acttatagag tatctttatc aaacgtggaa gaaagacact
     9481 gataagcagc agctttttga gaaatatgat agatatttta gacaaaagaa tgttgatatc
     9541 caaaataatt cagctttaac atatcccacc tatcaagaga ttcgatttag acagtatgtt
     9601 aaggaaatag agagagattt tcaaatagaa tttgactatt attatcttca aataacgtct
     9661 atctttttga attcttggat ttatcaaatc caattaacat cggacgataa attatattcc
     9721 ttcatcaatg atattaatag ttggtgtagt cgagaagttc aattagtcaa tcgttttatt
     9781 caaatagggg atatttattt aacaaaaatt caaaaaaatg ttttggttat tttatttaag
     9841 ttaatgtgta gtaaagaatt tcagcaaaaa cgtttagcac tgattttggc tcatggagat
     9901 ctaacggcaa cctgtatagc aagtactgct aataggatcg ttgagagata tatatacgac
     9961 gccattgata tgccactaat gacttcaagt agtgagattg cagacaaaat taatagatac
    10021 ttacaagagt gtaccggata tgaagaaatt attttacttg ttgatatggg ttcactggag
    10081 aaaatttatc taaatttgga actagacgct caaaaaaaat tcggactact aaacaatgtt
    10141 tcaacaagaa tggctattga tgttgcaaat cagatattat tagatttacc gcttaaacag
    10201 attcttgaac aagtaaaaga aaatcaaaat gtggattata tttatgaatc atcaaatatt
    10261 aagaaggata tgattgtctg ttcttgtgca agcggattag gaacttcgtt taaattaaaa
    10321 aagatacttg aagatagctt tccaagggat actgatcttg tcatagaaac gtgtgactat
    10381 tactctttag tgaatgaaaa ctatttatcg gagcttcgta aagagaacaa tattcttttt
    10441 attgttggaa cactaaatcc acatctagaa gatgtagaat tcttttcaat tgaagatatg
    10501 attttaggaa tgaatataag taaattctcc aatcaattga atagacactt taatgataat
    10561 gaactgaaag ttttgaacaa taatattctg aagaatttta gtctaacgaa tttaatggag
    10621 caattgacca tattaaatcc gactaaatta ttagaacggg tttcagatgc tatatacatc
    10681 ttacaaaatg atctaggaat ttcatttgat aataatacgt gtttcggatt atatgttcac
    10741 attagttgct taattgaacg attagtaaaa caaaatactt tggaagatga aatttatttc
    10801 aatgaaacga gtgaggaatt tcaaaaattt cagacacatt ttaagcaatc tttcagtgtc
    10861 gttgaacatt attatagtgt cgacatcccg attcatgagg ttaaatacgt ttatgattat
    10921 gtaaaaagag cttgatttat caagttcttt ttgttttggc atggtaattg cttgtaagaa
    10981 aatgaaagat aaaggaggaa gtatttatga gtattgttat gtcgagagtt gataaccgtt
    11041 tattgcatgg gattattgtc acgcaatggg caccaatgtc taaagcaaac agagtgatgg
    11101 tgatcgatga ttctgtcgca aataacgaca tccaaaaatc aagtatgcgt ttagctagac
    11161 ctgctggaat ggctgtatca attatcactg aagaaacagc gattgcgaat tttaaaaagg
    11221 ggaaatacga tcaggaaaaa gtatttgtca tcgtgaaaga gccggaaaca atccttcatt
    11281 tagtagataa cggtattaga attgaccgac taattgttgg cggtactgtt gttaaagata
    11341 atgcattaca gctatcttct agagcctatg catcagatga aaatatcagc acgtataaaa
    11401 aattgttgga ttctggtgta gaaattgtta cacaatttgt tccggcggat aaggcacagg
    11461 aaattcaatc aaaattagga taaaggagta aggataatgc attttactat ttttcaaatt
    11521 attatgctga ctatattagc atttattaaa catgttgatt attatgggat tccgatgatc
    11581 tttgtaaatt acgcagtttt ttggggatta ttcacaggaa ttttgatggg tgatataaaa
    11641 acaggtttga tagttggcgg tacagttcaa ttgatgtcat taggagttgc cggttttggt
    11701 ggctcatctg taccggacta tggaacgtgc gcaattattg caacggcatt tggtagccag
    11761 cttggagtag aagccggatt agcaatagga ctacctgtag gtatgttagg ggtgcaacta
    11821 gatgtaattg tcaaaatctt aaatggtttt gttgttcgca aatcacagga atatgcgaat
    11881 aaacgagaat ttaagaaaat gaatagtgtt ttgtggttgg ggccaatttt ctttggttta
    11941 tctgcagcaa ttccagtatt tattgccgtt actttaggac agtctgctgt taattacgtt
    12001 ttggatgttt tacctaattg gtttatggat gggctaacat tagccggaaa aatgttgcct
    12061 gctgttggta ttgcgatgtt gttacgcttt atgcctacag gtcgatacat tagttattta
    12121 cttgttggat ttttcttagc cgcttttttg aaggtaccta ttattggagc ggcgatcata
    12181 ggattcgctt tagcatttaa tgtgtatcat agagctgaaa aagatgcaca aatcactgct
    12241 tctgcgggag cttctgatgt tgggtttatt ggggaggatg aataaaatga gcgaaaaata
    12301 tcaagtaacg aaaaaggatc ttaggaaagc gaactatcgt tggttgatgt cagtttgtac
    12361 ctttaattat caaacacagc aaggtgcatc tgtgacttac gcattgtcac ctattttaag
    12421 aaaattatac aaagatgatg atgaatacat cgaagcttta aataatcact ttcagtatta
    12481 caatacacag ccttggctgg cagccattat tttaggtgct tgtgttgcaa tggaggaaaa
    12541 gcaaggatta gaagcaaaag atgcgattaa tgactttaaa gttggtacga tgggtccaat
    12601 tgctggtatt ggggactctc ttttgatgac gatgatccca accatcatgg gatcaattgc
    12661 ggcttatatg gctttagaaa ataatcctgt aggaatcttt ttatggttca ttttaatcat
    12721 ggtaatcttc tttttccgaa tgagaggctt tgaatttggt tataagcagg gaatgaagat
    12781 tgttactgaa tacggtgaaa agattaatta tttaactgaa gctgcttcag tattggggct
    12841 aacagtagtt ggatcattaa ttgcttcagt tatcagtgtt tcaacgccac ttaaattcac
    12901 ttttggtgaa gttagtatga aaatccaacc aatgttggac aaaattttac caacattaat
    12961 tccagtaatt attactgctg cagcatatta tctactcagt aagaaaaaaa tcagtatgac
    13021 cattttgatt ctactcgtta ttgtttttgc gatggttatg gctgcatttg gaatattagc
    13081 gtaaaggaaa aggatttgaa ttatgagaag attgatcatt gctagccatt caatactagc
    13141 tgagggtttt aaagagacac tagagttttt aacaggtatg cataatcaaa ttagtagctt
    13201 atgtgcatat gtcgaagaaa ttgatgatat agatgaacaa attaaagagt tagtggatac
    13261 tacagacgaa ataattgttt taactgacat ggctggagga agtgtaaatc aaaagttttt
    13321 tacctatatt tctgacagag tgcacgtaat tgcaggaatg aatcttccct tggcaatgtc
    13381 aatcgctttg cagttaggaa atgataattt agatattgga gcaattattg cggaggcaca
    13441 acaacaaatc atctatatga ataatcaaaa aagtgaagaa aggatagagg acgaataaaa
    13501 tgaattgttt aaataatata tggctccaca agggagagga aagtagaact atttcaccag
    13561 aaaataaaaa tggtgaaaaa gggaaagcgg caatgacagc gagtcatcta ggaattggga
    13621 gaaaagggtc accttctgtt ccgataaatg ctggagagac aattacttta gcagaaatgg
    13681 aaggccctgg aattttacgt catttttgga tgacagtgac agacaaaaca atcgctggga
    13741 gtttcgtctt acgtgattta attttgcgta tttattggga tgatgaatcc actccttctg
    13801 tagaaagtcc attaggagat tttttttgta atgggtttgg gacaagatgc gaagtgaatt
    13861 ccttaccaat cgtagtgaat ccaacatcag ggatgaattg ctattttgag atgccatttc
    13921 gaaaaaaagc taagattact ttaacgaatg aacatcctga gtttattaag cacatttttt
    13981 atacatttaa ttatgcattg actgaagtac cggatgatgc gatgtatttt catgctaaat
    14041 ggaaccgtga acccatcacg acaaaaggaa aagattacac ccttattgat ggaatcaaag
    14101 gcactgggta ttatgtagga acctactttg ctttgtgcgc attggaaaga tactggtggg
    14161 gggaaggaga atttaagttt tatatagatg gagatgaaga tttccctaca ataacatcta
    14221 caggagcaga agattatttt ggcggcgctt gggctttcca taaaaagaac tttggagaaa
    14281 gggcatatgc tcaaaatttt aatacgctat ttatgggcta tccttttcaa tctaaaagag
    14341 atcacacccg ggattatttt agtgctggaa aacctaatcc aaatccagtt catggttttg
    14401 gcgatgatgc tttaccgatg catgggttgt accgctggca tttacccgac cccattagtt
    14461 tcaaagaaaa tttacgtgtt actttccaaa ccctaggaaa tgatgacatc aagctttatg
    14521 agcgtcaaga cgatatttct tctgttgcct attggtacca aaaggaacca catgagtttt
    14581 tcgaaaaact attagacagg gaagaacgaa ttcctagata aaaggacggt ggagtaagaa
    14641 atgtatggct caatagaagc aggtgggact aaatttgttt gtgccattgg caatgatgat
    14701 ttaaaggttt tagaaagagt gagttttcct acaactacac cgaatgaaac aatgagtttg
    14761 gtaattgatt tctttaatca ctataaagaa caattagaaa gcattggagt agggtcattt
    14821 ggaccgattg atatccatcg tgaatcaaag acctatggac atatcacctc tactccaaaa
    14881 actgcctgga aaaattttga ttttgttgga acgttgaata agcatttcga aattccaatt
    14941 gcctggacaa ccgatgtgaa tgctgcttgt tacggggagt atgtttcagg tcatggaaaa
    15001 ggtttatcca gtgttgttta ttacactgtt ggaactggaa ttggtggagg agctatacaa
    15061 gatgggattt ttgttgaagg gtttagccat ccagaaatgg ggcatacgtt agttaagaga
    15121 cattcaggag atactttctc tggtacttgt ccttttcatc atgattgtct cgaagggttg
    15181 gcatctggac ccgctatcga gatgcgtacg ggaaccaagg gacaagatct ttctatagaa
    15241 gatccgttct gggaaatcga ggcggattat attgcacaat gtgcctgtaa tacgaccctg
    15301 atgctttcac cagatattat aatttttggt ggtggtgtca tgcaacaaga acatctgaaa
    15361 aagaaagtac aaagacgttt tttagaacta attaacggct atgtcgatac gccaaatatc
    15421 gaagaataca ttgtcacacc taagttagca gataatgcag gaactatagg atgcttaact
    15481 ttagcaaaag atgttagaat caattcttaa gcaaaaaagt attagtcaat aaaatgctta
    15541 tgaagtccga tcaacttaat gtggtcggac tatttgtatt cagcaatatc attcactcat
    15601 gatacccagc gccttatcat aagtgatgcg gtttttatca ttgatcctaa aaatcttagc
    15661 aagacaaaaa gtacgtaaga aagtaaagac taaatacttc ggaggaacaa atgaataaca
    15721 atgataggtt attgcgattg cgttatgcga ttgatttgaa agatacggat ttgattaaag
    15781 cttttgagct aggcggtgta ccactgacta aagaagaagc tcaggcggta ctgaccaaag
    15841 tacaggataa gaacaaagat aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc
    15901 aagtttttga cgcattccta aatggcctga ttctcttggc gcgtggtccg caaaaagata
    15961 agccgatagt agacacgccg caaaaaagca aggaaatcaa atatatcaac aatgtgttgc
    16021 taaaaaaatt aaaaatcgcc ttgtctatga ccactgatga tattttagac gtttttgctg
    16081 aggcagaaat ctatccatca aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac
    16141 gaaattttaa gccttgcgga gataaatata tgcgtaattt tctcaagggg ttaggaattt
    16201 ataatcggag aaaagtataa agaaatttca tgagaaatca aaacaatggt tgttttgatg
    16261 agagctagtt tttgtatgag attaaaatat tatcttaatc gaggagctgg attggtttta
    16321 tgaatgatga atataagaat gatgaagata agatgctttt tgaagagata gagaatcgat
    16381 gtaggctcaa ttttgagctt cggggaaaga tgtctttaat acaacaaaaa aagtatttag
    16441 cgaataaatc agagtttact ctgggacatg ttgaaaaatt aatctcggat tggattagta
    16501 gtagaagcga atttacaaaa ataaaacagc cgattaaatt tgacatgaaa aaacttttgt
    16561 taaataagag tgagataggt aatagagatc aatatattag agctaaaggg caagaaataa
    16621 ttgattcatt aggtgaaatg aggtcataca attatttgta tgtaacacat agagctgatg
    16681 gaatggtcat tactgtgggg aaatcttctt ctaatgatat ttttttagat ggagatcttt
    16741 tttatcaact caatacaaat cacttgtctg gcactgaaaa tataatttta aggacggaat
    16801 atggcaatga aatatttgca aaatatgatg agattttaaa aaattatttg gactgggctt
    16861 ggataattcc ggttgagtca ggagatgcta agaaactgga gagattattg ggcgatgaac
    16921 taattaataa aaaagtacca attttaaatt attattccca tagacaataa aaggctattt
    16981 ttcatgataa tactagttat aactgtaaaa atggaagaaa atctgggaaa gaagttttta
    17041 aataagtgta ttgttcgatt ttaataaata ataagcaata ccatccgtct gtgttgcgca
    17101 acatcgtact acaatgaata ttttttcaca aaaagaaaat catccatcga atgaatggaa
    17161 ggttttagta tacacgaggt taatgccccc tcttttataa tcagaggagt gaggtttttt
    17221 ctatgaatgt ttctattttc ttattgctag tctagtttct cttacgatga aattgggatt
    17281 ctattccagt ttttattgat ttgctgataa aataacttga atggtggata taaacatgat
    17341 aaataagaga agaataacaa aacaaggttg tgaaatgatt ctcaaatgaa tataatagaa
    17401 agaaaaagga ggaagaatca atgaaaatag caattgcagg cgctggcgca atgggaagtc
    17461 gctttgggtt aatgcttcac caaagtggga acgaagtatt attaatcgat ggctgggcag
    17521 aacatgtcca acaaatcaaa gagcatggat tacaagcaaa ttttaatggt aaagaggtag
    17581 aagcaaaact accaatcgtt cttcaatccg aagtagaaaa agaagatcaa gttgatctga
    17641 ttattctatt taccaaagcg atgcagctgg aaaaaatgct gcaggatatc caatcattaa
    17701 tcaaaaaaga tacagaggtc ttatgtctat taaatggtat cgggcatgaa gatattattg
    17761 agaaatttgt accaatggaa aatatctata ttggaaatac catgtggacg gcaggtcttg
    17821 aaggtcctgg tcaggtcaaa ttatttggaa gtggttcagt agaattacaa aatttaggtg
    17881 atggaaaaga agcagctgca aaaaaattag cagacaaatt gtctgaatca ggattaaatg
    17941 ctcatttttc tgacaatatt cactattcta tttatcgcaa agcatgtgtg aacggaacaa
    18001 tgaacggact atgcacaatc ttagacgtta atatggctga gctaggaaaa acatcgactg
    18061 ctcataaaat ggtggcgacg attgtcaatg agtttgccaa agtagcagca gtagagaaga
    18121 ttgaactaga tgtcccagaa gtcattgcac attgtgaatc ttgttttgac ccggaaacaa
    18181 ttgggttaca ttatccttca atgtatcaag acttgattaa gaaccatcga ttaacagaaa
    18241 tcgattatat caatggcgca atttctagaa aagggaagaa atatggtgtt gcgacgcctt
    18301 attgtgattt cttgactgag cttgtccatg caaaagaaga tagtttgaac gtaaaataaa
    18361 ggagagattg aatggaaact tcacaaaaaa tatcaccgaa aatttttctg aataaagtcc
    18421 tcgctggtac agcaacagga atcattattg gattgattcc taatgcagta ttaggtgcca
    18481 ttttaaaata ttttagtgcg tatccctttg cggtaacgat cgcacagtta gcagttattt
    18541 ttcaactagc aacacctttg atcattggtg gtttgattgc cttacaattt ggatttgatc
    18601 cgatgagaat gatggttgta gccggagctt cttttgttgg ttctggtgta gtcaaattca
    18661 cacctgattt gggtgaaacg ggtgtttacg ttggtgctgg tacaggcgat attattaata
    18721 cgatgctgac agcatcgatt gctgtcgggc tgattttact gattggaaat agttttgggt
    18781 cagtgtcgat tgtgttaaca ccgattgttg taggtgtagg tgcaggttta cttggttttt
    18841 atttattccc ttttgtaaca gcaattactt ctgcaattgg atcattgatt aataacttca
    18901 ctaccttaca accgctgtta atgtctatct tgattggttg ttcctttgcc tttttgatta
    18961 tctctcctat ttcaacagta gcaatcggaa tggcaatcca actagatggt gtttctgctg
    19021 gtgcggctgc gatgggcgta gctgcaacca cttttgtttt agtagtgaac tcttggaagg
    19081 ttaataagtc aggtgtgaca attgcgattg ctttaggcgc tatgaagatg atgatgccta
    19141 atctatttag aaaacctgtg attcttattc cttgtttagt tagtgcagtg attacagcta
    19201 ttcctgtcgc tctattttct atttcaggta cgcctgcttc tgctggtttc ggcgtggtcg
    19261 gacttgttgg tccgttggct tctttagatg cagggctaaa tggctttctt gttgttcttt
    19321 gttggttagt catccctgtt tttgcggcac taattactca atttttatgc gagaaagtat
    19381 ttaaactgta tgatcgagaa gaagtcttca aatttttagg ctagtagatt ttactaagaa
    19441 attttcaatc aatggatgcg taagtgagat gtcaacaaca tctgacttac gtttttttgc
    19501 ctttttgagt aggagattga tgctgacaac tgaactcttc agtgccattc gattttttaa
    19561 aatgtagaag aaactcacct ttatgttatt gattgttcaa aaggcaacta gaataaaaat
    19621 atgtgttaaa atgagaaaaa atttgattgt tggatgtatc gaataatcac taaaaagtag
    19681 gagaagaaaa tgattttttt gcaaaaatat ggtttttatt ttttattatt aggtgtgatt
    19741 agtgatttat cgacaccgta tatccttggc cttttttatc ccaaactgaa tcaaatgact
    19801 accgtaatta gtgtgtttgg tgacgtagat agtccagtca gacgtgcttt cttggtctgg
    19861 tcagttgtat ctggactttt ttttgttctt tcgttgcctg cgttgtatca cctatttatt
    19921 ggaacatcaa aaacacttgc aatacttgct gttgcaacag ttggtttata tgggataggt
    19981 gattgtattt ttactgggtt attcagtatc aatacgaatg aaagctcttg gaatctatcg
    20041 acttggatcc ataatatagg ttctgggcta ggctatgcag gctttctact tttcccattg
    20101 ttacttgttc tgctttatcg tcaaagtggt caaggtacgc ttagccattt ttatcttgtt
    20161 ttaacagtta ttagtttact aattgcaggt ctttacggat tagcacggat tccgtcgatt
    20221 agtcagtttg ccttctttaa gcaattgggc ttctttcaac gcttaagttt tttctttaac
    20281 tatttgccga tgatttgttt aagttttttt caactacgaa agtagaaaaa gcagtgtgaa
    20341 gctgattaag taaaagaatt tctctatggg taaccattcg tgtggatgtg ttcgactaag
    20401 aatagagatg aggagagcga aaagcaagtt agtcttaact tgttatgcgt tgtgctaaat
    20461 ttcaggtagt cattttttga atgaataaga agaaaacttg aaagagggtg acttcatgtt
    20521 agatgtggag cagatggaaa tagaccttta taatattatt ttaaatcgaa atacaagaga
    20581 atgggaaaga acacaatttg aagaggcaaa agaagaccta gcaaaaatgt cgataaagca
    20641 ggtgatagaa aaactagagg tcaacttgcg tccgctagca ctacgagata atttgacacc
    20701 agatgcgatg gatttttatt taagaatcac aggggagtca gcgagtgcaa aggcttatga
    20761 ttatgccaaa cataagataa cttcaccctt ataccaagaa cgggcgattt ttgccggtgg
    20821 ctgtttctgg tgtatggttg agccttttga aacaaaacca ggtgtaatct cagtgttatc
    20881 tggttataca ggtggagaga ccaaaaaacc aaattacgat caagtcagtg gtggatatac
    20941 tgggcatgtt gaagctgttg agatcatatt tgatacacgt caaataacct atgaagagct
    21001 gttggagatt tattggcaaa tttcagaccc tacagatgcc tttggtcaat ttcaagatcg
    21061 ggggaaacaa tatcgttcaa ttctatttgt tgttaatgaa aagcaaaggc aattggctga
    21121 aaaatcaaga gaacaactga ttcaatctaa ccgatttagc cgtcctatcg ttacagagat
    21181 tcgccaagca aagacttttt ggccagctga aaatcatcat caacaatttt atcagaagca
    21241 acctaaaaga tataaaaaga tcaagcattc gcatcagctt catttatttt ttcagcgaca
    21301 tagatagagg aaggttgtgg cgcaaagcgt tcagctccga caaaataatg agcaactgac
    21361 aaaaatagct tttcatattt ttggacagtt atgaattatt tcgaggagtt gctttgagaa
    21421 caccgaggaa aaggttgtgg cgcaaagcat tcagcttcaa gtcgcaagga aacaggaggt
    21481 atgacaagtc ttttgtcaca actcctgttt ttttactagc ctttatttcc atgtgaaaga
    21541 gaattcgaat tgtagaatct tgatttttct atggaatttt gtagagaatc atcttgataa
    21601 ttaatagaca cctaatcgaa ttccacatta atgatgaact aaattccgta taaaagcgca
    21661 aacaataact ata
//
LOCUS       DAZBWR010000045        21360 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000045 DAZBWR010000000
VERSION     DAZBWR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.045"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..741
                     /locus_tag="ACHGT5_001441"
     CDS             40..741
                     /locus_tag="ACHGT5_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN7217430.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1350..2366)
                     /gene="tsaD"
                     /locus_tag="ACHGT5_001442"
     CDS             complement(1350..2366)
                     /gene="tsaD"
                     /locus_tag="ACHGT5_001442"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN7217431.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2363..2827)
                     /gene="rimI"
                     /locus_tag="ACHGT5_001443"
     CDS             complement(2363..2827)
                     /gene="rimI"
                     /locus_tag="ACHGT5_001443"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN7217432.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2834..3376)
                     /gene="rimI"
                     /locus_tag="ACHGT5_001444"
     CDS             complement(2834..3376)
                     /gene="rimI"
                     /locus_tag="ACHGT5_001444"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN7217433.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3360..4082)
                     /gene="tsaB"
                     /locus_tag="ACHGT5_001445"
     CDS             complement(3360..4082)
                     /gene="tsaB"
                     /locus_tag="ACHGT5_001445"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN7217434.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4273..5253)
                     /locus_tag="ACHGT5_001446"
     CDS             complement(4273..5253)
                     /locus_tag="ACHGT5_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7217435.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5277..6761)
                     /gene="galT"
                     /locus_tag="ACHGT5_001447"
     CDS             complement(5277..6761)
                     /gene="galT"
                     /locus_tag="ACHGT5_001447"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN7217436.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6773..7762)
                     /gene="galE"
                     /locus_tag="ACHGT5_001448"
     CDS             complement(6773..7762)
                     /gene="galE"
                     /locus_tag="ACHGT5_001448"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN7217437.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8010..8177
                     /locus_tag="ACHGT5_001449"
     CDS             8010..8177
                     /locus_tag="ACHGT5_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN7217438.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8239..10050)
                     /locus_tag="ACHGT5_001450"
     CDS             complement(8239..10050)
                     /locus_tag="ACHGT5_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN7217439.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10047..10412)
                     /locus_tag="ACHGT5_001451"
     CDS             complement(10047..10412)
                     /locus_tag="ACHGT5_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN7217440.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10575..10970)
                     /locus_tag="ACHGT5_001452"
     CDS             complement(10575..10970)
                     /locus_tag="ACHGT5_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN7217441.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10988..11950)
                     /locus_tag="ACHGT5_001453"
     CDS             complement(10988..11950)
                     /locus_tag="ACHGT5_001453"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN7217442.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11950..12162)
                     /locus_tag="ACHGT5_001454"
     CDS             complement(11950..12162)
                     /locus_tag="ACHGT5_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN7217443.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12183..12881)
                     /locus_tag="ACHGT5_001455"
     CDS             complement(12183..12881)
                     /locus_tag="ACHGT5_001455"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN7217444.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12901..13443)
                     /locus_tag="ACHGT5_001456"
     CDS             complement(12901..13443)
                     /locus_tag="ACHGT5_001456"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN7217445.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13575..14579)
                     /locus_tag="ACHGT5_001457"
     CDS             complement(13575..14579)
                     /locus_tag="ACHGT5_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN7217446.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14576..15565)
                     /locus_tag="ACHGT5_001458"
     CDS             complement(14576..15565)
                     /locus_tag="ACHGT5_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN7217447.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15562..16368)
                     /locus_tag="ACHGT5_001459"
     CDS             complement(15562..16368)
                     /locus_tag="ACHGT5_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217448.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16534..17490
                     /locus_tag="ACHGT5_001460"
     CDS             16534..17490
                     /locus_tag="ACHGT5_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217449.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17567..18085)
                     /locus_tag="ACHGT5_001461"
     CDS             complement(17567..18085)
                     /locus_tag="ACHGT5_001461"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN7217450.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18173..18322)
                     /gene="rpmG"
                     /locus_tag="ACHGT5_001462"
     CDS             complement(18173..18322)
                     /gene="rpmG"
                     /locus_tag="ACHGT5_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN7217451.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18550..18996
                     /locus_tag="ACHGT5_001463"
     CDS             18550..18996
                     /locus_tag="ACHGT5_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN7217452.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19191..21086)
                     /gene="asnB"
                     /locus_tag="ACHGT5_001464"
     CDS             complement(19191..21086)
                     /gene="asnB"
                     /locus_tag="ACHGT5_001464"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN7217453.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6417 a         4544 c         3691 g         6708 t
ORIGIN      
        1 gacaataaat atagtgaaca ataaggagag gactttttaa tgcgtgttaa taaaatcgat
       61 tttcactttt caaaactcag atatcaacct gattttgaac atttctcgga tgaagagttc
      121 caacagatca aggaacatac tgttcttcgt tcttataaaa aaggacagat ccttttcgac
      181 gatggtgatc ccagacaacg gttgtatatc ttgaccaaag gactcgttcg tttagaacgt
      241 tatgatgaaa gtgcaactta ttactactat gactatgtga atacaaataa cctttttcct
      301 atgtcaggaa tgtttggaga agagacctac acttatacag ctcaagcgat gactgatatt
      361 gaaacattct accttcctgt ttcctactat gaaaaattag cacgaaaaaa tcccgaacag
      421 ttgatctata tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa
      481 atcggattga cctctagcgc ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa
      541 gaattgggcg taccaacaaa agatggtcat atcaccattc cttatccgat caccttaaaa
      601 gaattagcag tgaatagcgg cacgacacgt gaaactgctg gacaagtaat caaacaacta
      661 cgtgattccg ggctgttaga ttatgacaaa aagcgtttta ctttctgtaa gacagctact
      721 actccagcta tgagagaata acgcgaacaa aaataagcag tgaccctgat aaatatcagg
      781 gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta gatcactgga
      841 caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt ttgcaaaaaa
      901 tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt attcgatatt
      961 cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat gataaataag
     1021 ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata gggctttgac
     1081 aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact cttcggtcac
     1141 aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt gtgaatcaga
     1201 gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg catacgcggt
     1261 aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt tcttattact
     1321 tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct tggatcagca
     1381 ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc tgctccaatc
     1441 attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac ctcagggagt
     1501 ttagctgcca aagcatcctg caatccttca cgcaaccctt gattggccgc tacacctcca
     1561 gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt agtaatcaat
     1621 acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag atcttctcca
     1681 cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct aaaactgaaa
     1741 tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt tccctgatga
     1801 gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct tccaactttg
     1861 tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata tgatccatct
     1921 tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat caatggaaac
     1981 tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt tacaggaata
     2041 agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat cagcaacgag
     2101 ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc cgacacacca
     2161 gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg gcggctagct
     2221 acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc aacgatgtta
     2281 gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca acttgattca
     2341 atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt ctgctcataa
     2401 tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat atacgaaact
     2461 tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct aaaaaaacat
     2521 gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt gcatgccctt
     2581 ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct attactttcg
     2641 agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa tcagagtgag
     2701 gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt ggataagcat
     2761 tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct ttttttctgc
     2821 aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca tcttcttttg
     2881 tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc cgataaagtc
     2941 tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag cgattcatta
     3001 tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc tgatatgccg
     3061 gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac
     3121 caatcattct tccattatca aagataccta gatatagatg aggaagcggt gacttcaatt
     3181 ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct cgttcgacat
     3241 ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga cgatcgattt
     3301 gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg gcatacgctc
     3361 taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct
     3421 gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac tggttcaact
     3481 aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg aactgtatta
     3541 atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca atcactcccg
     3601 acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga agcagaaata
     3661 tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc cccagcgtat
     3721 acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc gatgcaatta
     3781 gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt ataagcaatc
     3841 gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc atcagaaacc
     3901 gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg gtcgattgca
     3961 ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc aagaatttgt
     4021 tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc taatgtaatc
     4081 atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata cttttttatt
     4141 ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat ctgctggcgg
     4201 gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcactccat tttcccacag
     4261 cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca caataatctt
     4321 tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg ctagctctcc
     4381 catccattcg gtataaactt ttacagtaga aagcgagggc gttacatatt tcgctacact
     4441 gacatcattg aagcccatca cagaaatatc ttctggtacc actaaacctt tttcttgaat
     4501 cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat caggaacatg
     4561 cggattcgtt tttaaatatt gcttcactgc ttgatatcct gactcgacag aaaaatctgc
     4621 ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa atgccataat
     4681 tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag caagcatagc
     4741 gatatgttgc gctttatggg agacaaagtg ttcaatgact ttttgaacac ttgacttaaa
     4801 gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca aaagcaaggc
     4861 attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta gtgcgatcgt
     4921 tccatctgcc ggaagatcac taatggaatc ccacgtttct ttcaataaag taacgtttgc
     4981 ttcttctgcc tttttttcga ttcctaggcg aatagataaa taataaagat cttcaagctc
     5041 ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg ctgcattttt
     5101 acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct ttgtggctaa
     5161 gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag gagaaactcc
     5221 tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt ttacgtttat
     5281 aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt gaatactcca
     5341 gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac atcggtgacc
     5401 gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg acgatgatat
     5461 tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag ttctggtttc
     5521 aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt ctcctttttg
     5581 atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc tgaaacattg
     5641 ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc aattggcgta
     5701 atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt ttcatccgaa
     5761 tatgctttcc atttttccaa tactgagtta gctgcttcaa taagcaagtt tttctctgtt
     5821 gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac ttgcggataa
     5881 tcttctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc atgacgtcct
     5941 gcctgataat gatcatgaga aagaatcgat cctccaacaa ttggtaagtc agcattcgaa
     6001 ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa tgtttccttt
     6061 gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt gtaataagca
     6121 taaggggaat attggaatcc ccagctttca ccgtctagat tcattcgaat aatccggtga
     6181 ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat gcaaagcatg
     6241 caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt tggatctttt
     6301 tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc tgccggaaat
     6361 ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact taaattaaag
     6421 aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa tgcattgacg
     6481 actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc ttgttctgct
     6541 ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc caataagtct
     6601 acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc tcctaccatc
     6661 cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc agcttcgatc
     6721 gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc ccctattctt
     6781 cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatc gctttgatat
     6841 ctgtgtattc aggtttccag cccagaatct ctctcgcttt ttcacttgaa gcgactaatc
     6901 ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt cctgtcactt
     6961 ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct aaattgaaga
     7021 agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca gcaatcaaat
     7081 cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa tcgtctccat
     7141 aaacagccaa ggcttttctt tgaccaagag ctacttgtaa aatgattggg acaagatgcg
     7201 tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct acattgaagt
     7261 agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc attttttcca
     7321 tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct tctgtgattg
     7381 gagactcttt cggctctcca taagttgctg cagtagaaga gaatacgatt ttattcacgt
     7441 tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg ttgaaataca
     7501 tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg attactcctt
     7561 caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg tctccttcat
     7621 aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta tctaccacag
     7681 ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata taaccggctc
     7741 cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt gtgtttgttg
     7801 attctatata atttaaaaaa cgaagtttac ttttatggtg aaaacaaaca aagtaaaatt
     7861 tcgtaaattc atatggtaaa tttataataa aacatttagt aaacattttc aagggttttt
     7921 tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt ccataataga
     7981 tataacaaat aataaatgag gtggattcaa tgaaaaaatt tgtatctgga atattagtag
     8041 gaagtctggc aacagccgct gccgttgctg gtttggttgc ttctgtgaaa aaaacagtga
     8101 tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgta
     8161 aacgcgtttc tcgttaataa ttatttaaaa atacgcatga agactttcta aaaaacagat
     8221 aagtcatcat gcgtattttt atttgtcagt caatataagt tgtgctattt tatctgcctg
     8281 tctcagattc catggatctg tataaggtct agctactgct gcagttagcc aatccacacc
     8341 ttcaacaggt attccatagc aataaaagtc agactggatt tcccctgaag catcaatcac
     8401 gcgattcgta cctcgctcaa catctatagc ttctgaatga taacttcctg tattatctga
     8461 aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt tttatgacag gattacttga
     8521 acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt
     8581 atcttgtaga gaaataacat agcggttgtg cttttgctga atatcaaatg gcggttcgat
     8641 caaatgaaaa atacccgcat ctatcagtgc tgctaattct ttcgtccgaa tgattggcgg
     8701 accgatcgtt aaaaagctat ttagatgagt aaaccatccc catagtattt ctttgtattc
     8761 cttagcagaa aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc agtcttttaa
     8821 tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct ttttccgctt cttttatctg
     8881 cccttcaata aatattcggc tagcttctga aaagggctca ttctgatagg caagaggagt
     8941 ttctaaatac gaccaatccc atctataagg gattagtgct ggatgctttc cgagacactc
     9001 ctctgaagag agggagagaa aatcatgctc aaaatttttc tttggaatcg gcaagtgata
     9061 ttcttcgatg attttcttgt aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa
     9121 tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc aggttttctt ttgttagtaa
     9181 ccgtggccat gccattgctc cgccttgctt ttgatttctt cctcgagggt aataagggag
     9241 cccccttctt gatccactgt atacaatcgg ttccttgcct gatggatgat agaccagttt
     9301 tcccttttcg ttctcgaagc gccctcctct tgatactgta aacaatccga cataatcaaa
     9361 aaaacttaag cctagacctc ttaaaaagac attttcttta ggcggtatct tcgaaagatc
     9421 tacatctgca ggattagaag gaagctgata cagtaaaaca tttttctttg cgtagtctac
     9481 ccacactctt tcttcttctg tctcatcatt ttcccagtga ccgcttgcta aaacaagctg
     9541 atcgatagaa tagctttgct gatctgtctg taaaagataa ccatcttttc ttttgcttac
     9601 tttttttact aaagaataaa caaactgaat agaagctgga aattcttctt gcaactttga
     9661 gaaaaaccag cgttgataga gtccgtataa acatcgagta gattgctgat ctttttccag
     9721 acgattgatt tcttctataa ttagttgctt atttttggtt accgtctgtt cgatataatc
     9781 tgtagcaggt cctttactcc attgaaacaa gttgggtccc tgtgttacca atcctccact
     9841 ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa acagaattca ttaataactc
     9901 cggtggctga ttcaaccgcc aaactcgacc tccaggacca ttgggatcga aaagaaaaag
     9961 ttcaagagaa tgtctgtcct gattattatg gagaatcctt tctgctacac ttaatcctct
    10021 aggtccggca ccaatgatcc ctattttcat ttgcatgcct ccatttcgat aaattttttc
    10081 aataaaacag catggttctg atgttcattt tttgcaccat aaacaaaagt aatgactgga
    10141 tgttctttca taagttgact gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc
    10201 aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt
    10261 ttgcgtgctt cattactagg tgcaatttct ttcaaccaca gatctaactg cgctttttct
    10321 tttgaaattc ctcgtggcca gaatctatcc acgagtattc tatagccatc ctctttttca
    10381 gcatcaaggt aaacacgttt taatttgatc atagttgttt ccttctttct accttttcgc
    10441 ttagcttata tatgtagata atagcacaaa ataaaaaaaa tgaacggcac aactatccat
    10501 tgcgccgttc atgtgatctt ggtcaaaatc taatgattga ttcggttttt aaagctgtca
    10561 tacaatcatc tgttttatac ttttttactt ttgatttttc cagaccatcc gtcatatcca
    10621 cccttaagta aataaatctc ttgataacct gctttacgta atttatttgc tgtacggatg
    10681 ctcaaacttt tcttttgatc gtaaatatag acaggctggt cttttcgaat agatcctaat
    10741 gaatctttca aaacagtgta agggatgttt cttgcaccta aaatatgccc agcatcaaac
    10801 acatcttttt cgcgaacatc gattacttgt gcttttctca ttccttctcg aaattcttct
    10861 tctgtcaaag tcgttgctga acgtttggcc attactcgta gatagacttc ccaaaggatg
    10921 attgcaagca caatcaatag caaaatgata ttaattaccc ataaaaccat gtaatgcctc
    10981 cgttttttta agcaaatttc aaggctaatg aggcagcacc aatgacacca gcttcattac
    11041 caagctctgc taatttgatt tttgttgatt ctcttacttg cgggaaagta aattctttaa
    11101 aatatttctc tacacggcta cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca
    11161 cgatacttga cggatttaat gtattcccca agtttccgca agctaaacct aagtagaagc
    11221 atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat
    11281 ctttacttgt tacttcttct ccgttgtcta gcattgcttt cagttttgaa tcccctgcgt
    11341 attcttctgc caagaaacga cctaggcgta ccacacctgt agcgctagat acagtttcta
    11401 aacatccgcg tttaccgcaa gtacattcga acccttcagg atccacagtg atatgtccga
    11461 tttctcctcc agctccgctc acgccgtgaa tcaaatttcc gccagcaacg atacctccgc
    11521 caacacctgt tcctagagta acgaatacaa cgtctggatc gttttctcca gcacctttcc
    11581 atcgttcacc taaagcagca acattcgcgt cattatcgat agcgaatttg attcctgttc
    11641 ctgcctcgat cagttttttc acaaattgaa cttctgtcca gtttaagtta tacgcaccaa
    11701 taaccgttcc agcttcgctg tccacgcttc ctggggtacc catgccgatt ccgataaagt
    11761 cttcggcttt catattatat aaattcaaac gatgattgat tgattcaatg atttcaggaa
    11821 cgatatgctt accatcatcc aaaatattgg tatcgatgct ccatttttgc tgaatctctc
    11881 cttctggtgt taaaattgca aattttgctg ttgttccacc taaatcaatc ccaataatct
    11941 tcttgtccat tacaaatttc ctttctttcg gctttcttca attcggtgct ctctttttaa
    12001 tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa cggctatcat acagattacg
    12061 caattcgata gtcattaatt caatgtcata gatgcgtgca cccacataga taaagatgcc
    12121 aaattttttg aacagctgct gtacatcata taagctctcc atctttaagc acttccttca
    12181 tattatacaa gtaaaccata tttttttaaa cccagtaaaa tacatattac aaaaagaaat
    12241 acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt
    12301 agacccaaaa cattccctaa cactaatccg ccaattagcc caccgacatg tccccaaaca
    12361 tctacagagg tatccagaag accaaaaaca aatgtcaaca caataaataa aagaaaatgt
    12421 ctgactagct gctggatcgc tgggttatgt ttaaaatgaa accctaagat aaacaacgca
    12481 ccgaacaaac cgaatatcga agtacttgca ccaccagaca gaacattcgc ttcgttaaaa
    12541 gcaaagcttg ctgtatttcc cattatcccg ctgaacatat aaatcaagaa aaaacgccaa
    12601 tggccataga ttgcctcgat ttgctgcccc ataaaataca gtatcagtga gttcattacg
    12661 aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta aacgccacca ttcattgaag
    12721 tgaacaagat aaggaccata cattccggca taataaggga tctcaagtcg cggaaataat
    12781 tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca taacggtcgt
    12841 cttaaccatg cacgcaattt tatttgctgt tgataattca tgaaaacctc ccatttcatt
    12901 ttagtcaata aacaattgtt ggatcttctg atcaaagctc tccggttgcc attgttcatt
    12961 caattgttca cgaaaaacaa ggctacatgt ttttttatga aaattattta aataccggtc
    13021 gtaataacca ccgccaaaac cgattcgata accggctcta ttgtagataa tacctggtac
    13081 aatgaggaga tcgatcatct ccggcaaata gagcttctct gattgtggct caagaacacc
    13141 aaaactcgaa cgtacaagaa cggactgttc atccatttca taaaacgcca tctgacgttt
    13201 cggcaaagtt ttaggtacaa ctattttctt ttgttctttt tctgctcgtt ggataaccgg
    13261 ctgtgtatcc agttcaaatt cattcgataa agtcgtccct atcacttttg ctgacttcca
    13321 gtactcgctg gcgaatagct gtttatagat tcgctgttct ttttcctttt tcttgccact
    13381 tttggccaga tttttcagct tttcgattcc tttccggcgt aatcttactt tcaattggtc
    13441 caaaaactca cccttccttc tctcataaaa tggtatagga aaaaaagaaa aatagcaatc
    13501 gaactttcaa ataaatgcat aaatagaata aaaagaagac ctagaaatga ttggccttcc
    13561 ttttttaatg aatttcaact gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga
    13621 aaatatggcg caccaaaaag ggcaatcaaa attcctgtag gtacttcccc acttggtaga
    13681 atcactctag caaccatatc tgcactaagc agaaaaatgg aaccgaacaa accgctcaga
    13741 acaaaggtcc agttattttc tttacgtacg actagcttcg cagcatgagg agctagtagt
    13801 cctacaaaag ataaactacc tgtcaccgct acactaccgc ttgccaaagc aacagctaac
    13861 aacaaaagga ttttttgact tttagccacg ttaaggccta atccttgcgc gcgttcttga
    13921 ccaaatgata gcaatacaat cctctgtgca taaagtcctc ctagcggaat taagataacc
    13981 gcccatggaa gcaatgccca aacataaggc cagctcgcac cccagattga tcccgctaac
    14041 catgcattca caaatccata attttcctta gataattgga tcgtgcatag caatgttaat
    14101 gcagaaaggc cggcattaac ggcaataccg gacaataaga ttcgattcat acccaaaaag
    14161 ccatttcgat cataggccgc aagatagaca cagcaagcag ctaaaacacc acctagaaag
    14221 gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc
    14281 actaaaaatc ctgctccggc attaatgccc aatatactag aatctgccaa ttcatttctt
    14341 gttactcctt gcaagatata accagaaacg ccaagacaag ctcctgctaa taaactgatc
    14401 agtaatcgag gaactcgaaa ttctctaaca atcagcgcca aagaagatga ttctttccct
    14461 atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg taccaatcat taaactacct
    14521 atagacagga tgacaagtac aaaaagcaag aaatagaacg tccaatatat ttttttcacc
    14581 gttgttccct ccttacttgt atcagtaaaa acggaacgcc aatcaatgca atgactaatc
    14641 caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc aacataacca
    14701 atccaccgcc aactaaaaaa gagacaggta gaacataacg atagtccgtt ccaaccattc
    14761 cgcgaacaac tggcggaacc aacagcccaa taaatgaaac aggtccgact aacgaaacgg
    14821 caatggctgc taacactgca actagacaga aaacaatcaa ccgcgttcgt ttaggatgac
    14881 ttccaagagc taatgcatgt gtttcactca aatgaatcaa agatattgct tttcctaatg
    14941 aaaaagaaac caaaacagca agtatatata aaggaaacaa tactgctaat tgtttccatg
    15001 tgatattggc agctccgcct acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt
    15061 gggtaagtgc ttggctgata gcagtaaaaa aagaactaat agctacgcca gctaaaatca
    15121 gctgtaaagg attcatgccc attttcgaaa actgcaaaaa accaaatacg agcaaagtta
    15181 cagcaaaaga acctgccaat gacattagaa acacagaaaa atcagaagct ttaggccaaa
    15241 aagcaaaact aagggccatt gccaacgaag cacccgcgtt gattcctagt aagcctgaat
    15301 ctgctaaagg atttcttgtc actccttgca tcaatgcacc gctcaatgca aaacaactcc
    15361 ctacaagaaa tgctcccgcc acacgaggca aacgaatatt tcgtatgatt tggtgacttt
    15421 gttttgatct atcaaaatca aacaacgcct gccaaaaatc tgctttttcc acagtcgctg
    15481 cgccatttga aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga
    15541 gtctattttt cttcatagtc ctcataattg atcctcctgc tcttttggca acaaatcata
    15601 ggttagaaaa actggtttat ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa
    15661 tatttttttc atacactctt ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt
    15721 accgtttttc attgctaata aataatctga aaaacgtgaa gcatgattaa tgtcatgaat
    15781 cgacatcaag atcgttgttt gtttcatttg attgatttca gtcaataact gtaggatctc
    15841 taattggtgt gccggatcta aaaatgttgt cggttcatcc aagatcaaga tatctgtttc
    15901 ctgagcaagc gccatagcga tccagacacg ctgggtttgt ccaccagaca gcgttgcgat
    15961 atcccgcata cgaaatccag ctagacctgt agcatccaac gcccactcaa ttgcttcatg
    16021 atcttgcgtg tccatgccac tgaacagttt ttgataaggg tatcgtccgt aagatactac
    16081 ttcttctact gtcatacctg tagggtgttc actagtttgt gctaataatg caattttttt
    16141 tgcaagttct tttgaactgt atgtagagat atcagagtta ttgacaatga tctttcctga
    16201 tttcacaggt agtatacggg taaaactttt caataaagta gattttccac ttccatttgg
    16261 cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca gacatgtttt caatgatttt
    16321 tttgttttcg tagccggctg caatgtcagc tgctcttaat ctcgtcattt cacccatcct
    16381 ttcgctgaaa atcattctca attatgacgc tgtttcaaca tttcgtcaag cgctactttc
    16441 atatctatag ccaaatgaag cggttgacag cgttcgaaat atcaggaata ataaccaatg
    16501 agaaagattc tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa agtattcaca
    16561 ttatctagca tattgctgct ggctggagta ctgagcgctt gctccccctc ttcagataaa
    16621 gaaacagcta caactgccaa ttcgacgact tcatccaata gtactcatac agtcacagat
    16681 acacttggac ataaagtaga gattccaaac gcgcccaaac ggatcatcgg cagttactta
    16741 gaagactatc tgattgcact aggggaaaaa cctatcgccc agtggactgt tggcagtggt
    16801 tccattcaag attatctgca agatgattta aaggatgtcc caacgatttc ttatgacctt
    16861 ccttatgaga aagtgttaag ctttgagcct gatttgctgt tgatttcttc ttccgctact
    16921 gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac
    16981 ggaacagatg tcacatggga agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa
    17041 aaagaggcag aaaaaatcat ttccgattat caaaaatcca tcaagaaaac tcgagaagaa
    17101 ctagcagata aaatcgagaa caagacagct gcagtgttgt gggtgaccaa caactcagcc
    17161 tttatggtca gcaaaaatcg gtcaagtgga agaatcgtat acgacgatct ggaatttggt
    17221 gttcctgatc tagtggaaga ggtctccaaa aaagccactt ctgactggtc tgctgtttcc
    17281 tctgaaaagc tagctgaatt agatgctgac tacatcatcc ttgtgaacag tgacaaagat
    17341 gctgccatgt tcaatgaacc aagctggaag aatctaaaag cagtgacaga caatcatgta
    17401 ctgcaattcg gacctgaatc tagttggcta tataacggac ctattgccag tcaggaaatg
    17461 gtcaatgata tcaaagaaaa actaaattga ttttaactaa aaaaagcatc ttcacgatta
    17521 gtcgtgagat gctttttttc ttattaacaa actagacgca tagattttat tcttcttttg
    17581 aagcaaccct attttttact ctttcccatt tttgaaaacg atgggcatat agattttctt
    17641 gatccgttat cccctcatac tcctctataa gggcgaagtc tgaaaaatca atattcccaa
    17701 tatacgtatc gccttcaaac gtgctatcga tcaatgttcg ccagatccga ccagtgtaag
    17761 gaagaagttc ttgaaaaatc cgactaccac ccgaaacata gatatcttct gagatcgttt
    17821 gactatacga caaaagttca tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa
    17881 aatttggttg cgttgtcaac acgatgatat ggcgataagg aagtgaaaga tcccccattc
    17941 cttcataggt ctttcttccc atcactaaaa ttcgatccat tgtatgttcg cgaaagaaac
    18001 gcatatcatt tggcaatcgc catggtaaca aaccattttt gccgatcaga ccatttttat
    18061 cttgtgccca catggaaata aacatattat ccgctcctta tgattttaga tgttttgaga
    18121 taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga actttaaaaa cattattttg
    18181 tttcacggtg caaagtaact ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat
    18241 caggattgtt acgtttgttt ttgcttgtta gatagttacg ttctttacaa gaagtacact
    18301 ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt tacaagaatc ctctaagatg
    18361 actctcagac ttgattatct taccatgatt ttttctagat tgctagtgtt tttcacgatt
    18421 ttgttaagta aaactacttg acgataaata caacggtttt acttctcaaa caaaatcttt
    18481 ttctttttgt ataaataccc ttacacttgt ttatatagat tatgtttttt aataaagggg
    18541 gatactaata tgccatggaa catgaaggac tttcctgcat caatgaaaaa cttagataaa
    18601 ttgacaagaa aaaaagcaat cgacatcgca aatgcattat tagatgaagg gtaccctgat
    18661 aaccgtgcga tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga tgctagtgaa
    18721 tcagaaaaac gaactttcga aaaagaaaaa aatccaagca agacggatga acacgatacg
    18781 aatcctcgtg ctggaaaact gcttgattca gatgtgatcg tagaatatga ggaagaacaa
    18841 tggatcgtga aatctaaagg ggcaaaaaag gcaagcaacc attttgatac aaaaaaagaa
    18901 gcaatcgaaa aaggaaagca agtagcacaa aacaaagaat caactttgtt catttataaa
    18961 aaagatggga caaaagaaaa agaaatcgat tattgaacat atgtaaaggg gttgtgacga
    19021 aaatcgtgtc acaaccccct tatgttcgaa acaatggtgt tcctctgcca actccgaacc
    19081 ataagtcata atatctataa aacgggaaga gctgtttttt gaaattgttt cttatcactc
    19141 agtgttaaac ggtaggagaa aacctctttt ttattgatat ttgattttca tcaaacagtc
    19201 acatagtcga ttgcttctgc ttgcggaatc gtttcatcta caaagtagac tttataccat
    19261 gtcaagaaac tgaagattgt ccagattttt ctttgctctt ctttacgtcc ttcgtgatgg
    19321 tcatctagca acttcatgat tttttcttga tcaaaaaatt cttttgcaaa atctgcttcg
    19381 aataatgctt tgacttgttc gtagccgtgt tcttctttca accaagcttt gattggcaca
    19441 gggaatccta atttcacacg attcgaccat tcttccggaa gatgtttgtt cgcagcttgt
    19501 ctgaaaatat cttttgtgtt gttttgattc aataaaaact tacttggtat ttgttgagcc
    19561 aatttcatga cttcgatatc caataaaggt actcggactt ccaaagaact tgccatagaa
    19621 agtttatctg cctttaacaa gatatctttt ggcatccatt gatgaagatc gacatattgc
    19681 atcttattga cttcatcttt gattccttct gttttctcgt aatgaacagt catgatttca
    19741 tccacagatg gtgctgtttg atattttggt gttaacactt ctgatgcttc tgattcttcg
    19801 aacactcgag catgaccgat aaaatattct tttgcaggag caagtgattc atataaatgg
    19861 atccgtccat ggaagttttt catttttccg attttacgtc ctaaattata gcgtgttcct
    19921 tttggtaatt ttttcaatcc ttgagcaatc acacggatga attttgaatt cgtatggaat
    19981 ccatacgttt ggtatcccgc aaacagttca tccgcccctt ctccagattg gactacccgc
    20041 acactttgtg aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg atttgaatca
    20101 ggttcatcta agtgatactg gatcaaaggg aatgctttga acgacatgtc tccgtcgatc
    20161 actgctgctg tattatctaa gtccaatagt tcggttagtt ttctggcttc aatcgcttca
    20221 ttataggttt tatcatcaaa cccgattgag aaagaatgat ctggtttcaa tacagaggtt
    20281 acataactgg aatcaacccc gctggataag aaagaaccaa cttctacatc actgaccgta
    20341 tgtgcttcaa tagaagcttg aacggtttcg tcgatttttt cgatccattc ttgtcgggaa
    20401 tgcgtttctt ttgtttcaaa atctgcatcc caatattgct gaatgtctat ttttccatct
    20461 ttatacgtat aataatggcc ttcaggcaaa cgatagacat ctttgaagaa tgtttcgccg
    20521 tttaatggag aatattggaa agtcatataa ggtttcaacg cttctttgtt caattctttg
    20581 ttaaaatcag gatgaggcag aaaacttttg atttctgaac caaacataaa tgttccgttc
    20641 atttctgcat aataatatgg tttgatccca aagtggtctc ttgcaccgaa taactcattc
    20701 ttttcattat cccaaatagc aaaagcaaac attccgcgga tcttttgcaa taattctgtt
    20761 ccccattctt cgtaaccatg caaaaggact tctgtatctg catgtgtttg aaaaacgtgg
    20821 ccagctgcaa tcaattcttc tctcaaaggc tggtaattat agatttcgcc attaaaaata
    20881 atgatctttg tccgatcttc attgtaaata ggctgagtac cgccctctaa gtcaataata
    20941 cttaatcttc tgaatcctaa agcaacatgt ttatcgatat attctcctga actattaggt
    21001 ccacggtgaa cgattcgatc catcattgta ttaataattg tttttttgtt gtccttgtcg
    21061 ttaacaaaac ctacaatacc gcacatttaa ctttcacatc ctaatataaa attactgttt
    21121 atttcattca tatgcatctt atcatagaat aaatagcgga cgaatatttt ctctcttaca
    21181 atttcgtcac gtgaaaacaa aagatgaaag tttctgtttt tacagaaaat aaagtcccaa
    21241 tatctaattt accatgtttt cttttttgag gctaccttat tctcttcgtg ttaaaaaaat
    21301 attagtggat gctatttcca aaaaaaccag caaaaaaaga cacgacctaa agatcgtgtc
//
LOCUS       DAZBWR010000046        21302 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000046 DAZBWR010000000
VERSION     DAZBWR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.046"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHGT5_001465"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHGT5_001465"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN7217454.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHGT5_001466"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHGT5_001466"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN7217455.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHGT5_001467"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHGT5_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN7217456.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHGT5_001468"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHGT5_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN7217457.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHGT5_001469"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHGT5_001469"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN7217458.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHGT5_001470"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHGT5_001470"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN7217459.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHGT5_001471"
     CDS             complement(8053..8523)
                     /locus_tag="ACHGT5_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN7217460.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHGT5_001472"
     CDS             complement(8569..8994)
                     /locus_tag="ACHGT5_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN7217461.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHGT5_001473"
     CDS             complement(9061..9330)
                     /locus_tag="ACHGT5_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN7217462.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHGT5_001474"
     CDS             complement(9341..10936)
                     /locus_tag="ACHGT5_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN7217463.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHGT5_001475"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHGT5_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN7217464.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHGT5_001476"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHGT5_001476"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN7217465.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHGT5_001477"
     CDS             15402..16376
                     /locus_tag="ACHGT5_001477"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN7217466.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHGT5_001478"
     CDS             16546..17946
                     /locus_tag="ACHGT5_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN7217467.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHGT5_001479"
     CDS             complement(18024..18746)
                     /locus_tag="ACHGT5_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7217468.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHGT5_001480"
     CDS             complement(18825..19331)
                     /locus_tag="ACHGT5_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN7217469.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHGT5_001481"
     CDS             19454..21253
                     /locus_tag="ACHGT5_001481"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN7217470.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLFQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5925 a         4668 c         3840 g         6869 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttttca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ct
//
LOCUS       DAZBWR010000047        20892 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000047 DAZBWR010000000
VERSION     DAZBWR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.047"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..3301
                     /locus_tag="ACHGT5_001482"
     CDS             23..3301
                     /locus_tag="ACHGT5_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN7217471.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3842..5749)
                     /locus_tag="ACHGT5_001483"
     CDS             complement(3842..5749)
                     /locus_tag="ACHGT5_001483"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN7217472.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5879..6754
                     /locus_tag="ACHGT5_001484"
     CDS             5879..6754
                     /locus_tag="ACHGT5_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN7217473.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6834..7808
                     /locus_tag="ACHGT5_001485"
     CDS             6834..7808
                     /locus_tag="ACHGT5_001485"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN7217474.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7988..9133
                     /locus_tag="ACHGT5_001486"
     CDS             7988..9133
                     /locus_tag="ACHGT5_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN7217475.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9270..9614
                     /locus_tag="ACHGT5_001487"
     CDS             9270..9614
                     /locus_tag="ACHGT5_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN7217476.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9885..11171
                     /gene="efmA"
                     /locus_tag="ACHGT5_001488"
     CDS             9885..11171
                     /gene="efmA"
                     /locus_tag="ACHGT5_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN7217477.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11309..11908)
                     /locus_tag="ACHGT5_001489"
     CDS             complement(11309..11908)
                     /locus_tag="ACHGT5_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN7217478.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12312..13433
                     /gene="mnmA"
                     /locus_tag="ACHGT5_001490"
     CDS             12312..13433
                     /gene="mnmA"
                     /locus_tag="ACHGT5_001490"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN7217479.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13679..15100
                     /gene="arcD"
                     /locus_tag="ACHGT5_001491"
     CDS             13679..15100
                     /gene="arcD"
                     /locus_tag="ACHGT5_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN7217480.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15114..16232
                     /locus_tag="ACHGT5_001492"
     CDS             15114..16232
                     /locus_tag="ACHGT5_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN7217481.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16292..17584)
                     /locus_tag="ACHGT5_001493"
     CDS             complement(16292..17584)
                     /locus_tag="ACHGT5_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN7217482.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17734..18294
                     /locus_tag="ACHGT5_001494"
     CDS             17734..18294
                     /locus_tag="ACHGT5_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7217483.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18423..18800
                     /locus_tag="ACHGT5_001495"
     CDS             18423..18800
                     /locus_tag="ACHGT5_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN7217484.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18733..20805
                     /locus_tag="ACHGT5_001496"
     CDS             18733..20805
                     /locus_tag="ACHGT5_001496"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN7217485.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6768 a         3792 c         4472 g         5860 t
ORIGIN      
        1 atttgttacg ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat
       61 gaatctcctt atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga
      121 acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga
      181 ggaaatacca agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa
      241 agaatcagaa aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg
      301 ggttgttttc ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg
      361 cgtgcggctc tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat
      421 gacctattat tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga
      481 aatagaatct tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc
      541 agtaccaaaa aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg
      601 cagtcggatc gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag
      661 caaatatacg atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga
      721 tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc
      781 agcagggttc ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa
      841 tctgtttccg gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac
      901 tggtttagag gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat
      961 gtatgactgg gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc
     1021 agcaaggaat atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac
     1081 agatcctcct gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc
     1141 tagtaatcat tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt
     1201 attgacaatt aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat
     1261 cactgcgaat cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac
     1321 tttcagtttt acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta
     1381 tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta
     1441 tgtaacacca gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac
     1501 acaagcattg agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc
     1561 cttcgaaaga ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca
     1621 gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg
     1681 aaatgggatg acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag
     1741 tgcagctatg caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca
     1801 tgcttatcgg aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga
     1861 agcatggccc gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg
     1921 atttgaagat gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga
     1981 cggttgtatc gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac
     2041 aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc
     2101 aggaattgag ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg
     2161 ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa
     2221 tagcggaaca gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt
     2281 ggctctttat acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt
     2341 ccgaacagag aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca
     2401 aatctaccac tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct
     2461 tggacatacc atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca
     2521 gcagcctgtt acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa
     2581 accggggaat cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc
     2641 tatttttgac ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga
     2701 accaaatcag acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg
     2761 tgtggcggag aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc
     2821 aactttcaca ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa
     2881 gatccaaatc gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc
     2941 agcaccttca aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg
     3001 ggaaaaatgg tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga
     3061 aactgccgat gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc
     3121 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3181 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3241 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3301 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3361 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3421 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3481 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa
     3541 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3601 taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3661 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta
     3721 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3781 agaagcgatt ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     3841 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     3901 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     3961 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4021 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4081 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4141 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4201 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4261 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4321 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4381 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4441 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg
     4501 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4561 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4621 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4681 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4741 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4801 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     4861 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     4921 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     4981 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5041 ccgtgacaag ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt
     5101 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5161 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5221 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5281 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5341 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5401 gtaatgtatc caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg
     5461 attcaacttt ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat
     5521 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5581 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5641 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5701 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5761 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     5821 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     5881 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     5941 agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt
     6001 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6061 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6121 gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat
     6181 tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt
     6241 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6301 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct
     6361 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6421 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6481 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6541 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6601 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6661 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6721 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6781 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     6841 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     6901 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     6961 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7021 aatcaacgaa tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa
     7081 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7141 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7201 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7261 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7321 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7381 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7441 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7501 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7561 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7621 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7681 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg
     7741 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7801 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc
     7861 tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     7921 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     7981 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8041 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8101 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8161 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8221 taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac
     8281 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8341 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8401 agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa
     8461 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8521 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8581 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8641 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8701 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8761 tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     8821 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     8881 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     8941 taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca
     9001 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9061 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc
     9121 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9181 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9241 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9301 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9361 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9421 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9481 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9541 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9601 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9661 gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca
     9721 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg
     9781 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
     9841 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
     9901 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
     9961 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10021 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10081 gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10141 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10201 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10261 tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga
    10321 atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg
    10381 ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt
    10441 ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag
    10501 ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag
    10561 ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca
    10621 gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag
    10681 tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa
    10741 catggaaaga ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg
    10801 gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg
    10861 caggagcagg ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga
    10921 gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg
    10981 caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa
    11041 tgttcttgtt ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag
    11101 ctgcccgaaa ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa
    11161 aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc
    11221 ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt
    11281 gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat
    11341 agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga
    11401 gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat
    11461 gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat
    11521 gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa
    11581 atttgggact tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc
    11641 gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca
    11701 aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa
    11761 catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat
    11821 ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa
    11881 agtgcatatc actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa
    11941 acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg
    12001 tgcagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta
    12061 ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc
    12121 tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag
    12181 aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat
    12241 gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg
    12301 atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg
    12361 tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat
    12421 tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata
    12481 aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa
    12541 aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac
    12601 ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa
    12661 tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa
    12721 acggcgtggt ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta
    12781 gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat
    12841 cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa
    12901 cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag
    12961 caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga
    13021 tgtattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag
    13081 aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt
    13141 tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga
    13201 atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg
    13261 ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg
    13321 tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg
    13381 gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat
    13441 taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag
    13501 ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa
    13561 agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa
    13621 aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat
    13681 ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc
    13741 aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc
    13801 agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa
    13861 tcatttagga gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc
    13921 atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg
    13981 aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa
    14041 aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt
    14101 attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa
    14161 attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt
    14221 cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg
    14281 aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat
    14341 cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc
    14401 gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg
    14461 cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa
    14521 aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat
    14581 tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga
    14641 cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt
    14701 agttgttact gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc
    14761 ctatcaattt gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg
    14821 gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg
    14881 gattgctgta gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt
    14941 atgtagtatt gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa
    15001 acatcaaatt acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct
    15061 ttcaatcggg ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa
    15121 tcgcaaattt cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata
    15181 ttgcacaaag ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta
    15241 gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag
    15301 gatcagatgt tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca
    15361 tcttacaaac gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag
    15421 caggagacca tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat
    15481 ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt
    15541 tagaacagct agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa
    15601 acaatcctac aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc
    15661 gtacagtaga tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg
    15721 aattcttgtc gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa
    15781 cctactccat tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag
    15841 tttttcgaaa gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg
    15901 ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag
    15961 agaaccttgt gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc
    16021 ccaattacgt ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt
    16081 gtatcaattt attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac
    16141 cgaagcatgc ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc
    16201 tcttatctga gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga
    16261 caactgttca agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg
    16321 aagctatgga gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg
    16381 tattgtgtcg tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc
    16441 cgcgataacc aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag
    16501 ttgcttggaa agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt
    16561 ttgtgatcaa tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac
    16621 gtaaggcagc taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg
    16681 caaaacccaa accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa
    16741 atcttggagc ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca
    16801 acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa
    16861 tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg
    16921 gatatttcaa gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga
    16981 caaatttttc atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg
    17041 taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc
    17101 caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt
    17161 cttttggcgt cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc
    17221 cttgaagcag acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa
    17281 tgacgattgc cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg
    17341 ctaattttcc ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca
    17401 atccgctcca aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg
    17461 acataaaagg catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga
    17521 caaacaagtt ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag
    17581 ccaattagac tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat
    17641 atgtagttta tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag
    17701 tgaaggattt attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag
    17761 aaaatcatcg actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt
    17821 cagcatttag ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat
    17881 attcgtgatg tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg
    17941 cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat
    18001 cgatcctttg ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc
    18061 aatgaagctg ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt
    18121 gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg
    18181 atccacggtt atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat
    18241 ccagaagaca gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc
    18301 agattcattt gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc
    18361 ttgcaacttt cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga
    18421 gtatgtatca agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact
    18481 gggaagaaga tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt
    18541 acgaaaaaca atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact
    18601 ttctcagcgc cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt
    18661 tacagcaata caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca
    18721 gaaaggaatt ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta
    18781 agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc
    18841 gttacttggg cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa
    18901 tggcagagct ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca
    18961 cttcctaaaa caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc
    19021 tcagaagcag tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc
    19081 ttagagtatg tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat
    19141 aaagcaatca aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat
    19201 gaatctgctc ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc
    19261 ttaactgtga agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca
    19321 ttagtgagag accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc
    19381 acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa
    19441 acacaagaag aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca
    19501 gaagtccaag aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc
    19561 caaagagaag ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa
    19621 agtttgtatg agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca
    19681 aatgacatga aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag
    19741 gtcaagcagt atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt
    19801 aaagtgaccg atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta
    19861 agtaatgaag agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct
    19921 caaccccata aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt
    19981 tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa
    20041 acagtcaaat gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta
    20101 tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt
    20161 ctgggaaccc cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg
    20221 gaacgtaccc ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta
    20281 aatccctctt tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt
    20341 gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc
    20401 ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag
    20461 aaatgcccag aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat
    20521 gtctgcacca attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat
    20581 cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc
    20641 cgttgtaaat acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa
    20701 atgaccaaac atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa
    20761 gaaagcccat tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag
    20821 gttgtgatag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca
    20881 aatttttgtg aa
//
LOCUS       DAZBWR010000048        20471 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000048 DAZBWR010000000
VERSION     DAZBWR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.048"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACHGT5_001497"
     CDS             complement(383..1849)
                     /locus_tag="ACHGT5_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN7217486.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /locus_tag="ACHGT5_001498"
     CDS             complement(2140..4224)
                     /locus_tag="ACHGT5_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN7217487.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4366..4488)
                     /locus_tag="ACHGT5_001499"
     CDS             complement(4366..4488)
                     /locus_tag="ACHGT5_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217488.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4749..5108)
                     /locus_tag="ACHGT5_001500"
     CDS             complement(4749..5108)
                     /locus_tag="ACHGT5_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN7217489.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5138..5479)
                     /locus_tag="ACHGT5_001501"
     CDS             complement(5138..5479)
                     /locus_tag="ACHGT5_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217490.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5476..6150)
                     /locus_tag="ACHGT5_001502"
     CDS             complement(5476..6150)
                     /locus_tag="ACHGT5_001502"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN7217491.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACHGT5_001503"
     CDS             complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACHGT5_001503"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN7217492.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8664..9854)
                     /locus_tag="ACHGT5_001504"
     CDS             complement(8664..9854)
                     /locus_tag="ACHGT5_001504"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN7217493.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9875..10402)
                     /locus_tag="ACHGT5_001505"
     CDS             complement(9875..10402)
                     /locus_tag="ACHGT5_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN7217494.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10449..13238)
                     /locus_tag="ACHGT5_001506"
     CDS             complement(10449..13238)
                     /locus_tag="ACHGT5_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN7217495.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13385..13585)
                     /locus_tag="ACHGT5_001507"
     CDS             complement(13385..13585)
                     /locus_tag="ACHGT5_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN7217496.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14038..14358)
                     /locus_tag="ACHGT5_001508"
     CDS             complement(14038..14358)
                     /locus_tag="ACHGT5_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217497.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14861..15979)
                     /locus_tag="ACHGT5_001509"
     CDS             complement(14861..15979)
                     /locus_tag="ACHGT5_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217498.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16212..16334
                     /locus_tag="ACHGT5_001510"
     CDS             16212..16334
                     /locus_tag="ACHGT5_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217499.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16450..17757
                     /locus_tag="ACHGT5_001511"
     CDS             16450..17757
                     /locus_tag="ACHGT5_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN7217500.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17878..19077
                     /locus_tag="ACHGT5_001512"
     CDS             17878..19077
                     /locus_tag="ACHGT5_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN7217501.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19104..20372)
                     /locus_tag="ACHGT5_001513"
     CDS             complement(19104..20372)
                     /locus_tag="ACHGT5_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN7217502.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5927 a         4356 c         3571 g         6617 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     5161 ttattcttct ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt
     5221 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca
     5281 cccgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg
     5341 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     5401 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg
     5461 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     5521 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     5581 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     5641 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     5701 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     5761 aaacggctcc atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt
     5821 tcgataaacg gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct
     5881 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     5941 taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     6001 gttcatgcat tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag
     6061 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     6121 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     6181 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     6241 taaaaaatga caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta
     6301 gttttttata ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc
     6361 cagagaaatc gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac
     6421 atatatttat aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta
     6481 gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta
     6541 aacttacata tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac
     6601 tattagtttg ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa
     6661 tcagatttcc atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata
     6721 gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc
     6781 ctttatcccg tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa
     6841 cgttgctctc cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact
     6901 gttttttgaa attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata
     6961 taaacggtgt tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa
     7021 gctatttttc cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt
     7081 ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7141 gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct
     7201 ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa
     7261 catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc
     7321 acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg
     7381 tcgataccag caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga
     7441 acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga
     7501 ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt
     7561 tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga
     7621 tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt
     7681 tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg
     7741 tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat
     7801 ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat
     7861 tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa
     7921 ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga
     7981 cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa
     8041 gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca
     8101 aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc
     8161 ggatcgattt tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca
     8221 tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc
     8281 atcaagaaat ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc
     8341 acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt
     8401 tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct
     8461 tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt
     8521 aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt
     8581 tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt
     8641 atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt
     8701 ctgcgtaccg cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct
     8761 ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct
     8821 acccattgtg tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa
     8881 agatagaaag ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga
     8941 tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca
     9001 cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg
     9061 gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc
     9121 cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt
     9181 gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa
     9241 atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt
     9301 tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt
     9361 gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct
     9421 tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc
     9481 cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta
     9541 tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca
     9601 tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt
     9661 gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct
     9721 ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt
     9781 aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt
     9841 tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt
     9901 catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta
     9961 ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt
    10021 caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat
    10081 ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat
    10141 catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg
    10201 tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg
    10261 ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata
    10321 cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg
    10381 gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga
    10441 cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt
    10501 tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg
    10561 ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc
    10621 gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc
    10681 tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg
    10741 cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc
    10801 aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg
    10861 aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct
    10921 ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg
    10981 aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa
    11041 taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag
    11101 atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca
    11161 atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata
    11221 taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa
    11281 tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa
    11341 tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca
    11401 aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata
    11461 atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc
    11521 aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc
    11581 atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct
    11641 tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac
    11701 cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt
    11761 ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt
    11821 actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct
    11881 ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa
    11941 aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac
    12001 tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc
    12061 aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag
    12121 accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta
    12181 aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga
    12241 tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc
    12301 gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga
    12361 taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct
    12421 tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt
    12481 gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa
    12541 tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct
    12601 aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa
    12661 cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt
    12721 ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca
    12781 ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag
    12841 cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt
    12901 atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa
    12961 taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca
    13021 tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt
    13081 tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct
    13141 gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc
    13201 gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc
    13261 tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa
    13321 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
    13381 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
    13441 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
    13501 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
    13561 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
    13621 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
    13681 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
    13741 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
    13801 ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa
    13861 actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg
    13921 aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca
    13981 gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta
    14041 tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc
    14101 tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca
    14161 aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag
    14221 atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg
    14281 tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca
    14341 taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata
    14401 tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg
    14461 tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct
    14521 tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc
    14581 ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct
    14641 tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt
    14701 tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc
    14761 tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt
    14821 ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg
    14881 tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa
    14941 tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat
    15001 cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt
    15061 ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat
    15121 tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga
    15181 tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg
    15241 gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg
    15301 gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt
    15361 tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat
    15421 ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc
    15481 ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt
    15541 tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat
    15601 agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt
    15661 gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg
    15721 ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt
    15781 cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt
    15841 tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat
    15901 tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt
    15961 ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc
    16021 taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg
    16081 tttctacggt tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt
    16141 tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag
    16201 aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg
    16261 ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta
    16321 aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt
    16381 gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg
    16441 agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt
    16501 tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat
    16561 gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga
    16621 gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat
    16681 caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa
    16741 aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc
    16801 gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg
    16861 gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa
    16921 gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt
    16981 ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat
    17041 gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc
    17101 actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac
    17161 tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg
    17221 actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc
    17281 actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca
    17341 gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat
    17401 gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc
    17461 gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta
    17521 acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg
    17581 gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat
    17641 cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca
    17701 aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg
    17761 aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa
    17821 ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg
    17881 aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt
    17941 tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac atggacgttt
    18001 gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg
    18061 attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg
    18121 ttgaatggtc ttttattcaa tattttcctc attagttttg gtatctactt gtttgtcttg
    18181 ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac gatcccgtta
    18241 ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg gaacggtgtc
    18301 acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat actagccatt
    18361 tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc acaatggata
    18421 aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt attctacaac
    18481 tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca agacttcatc
    18541 gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct tgccaagcga
    18601 atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa tccgcctatc
    18661 ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa
    18721 caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac caattcgaca
    18781 accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc
    18841 tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct ctatgcacga
    18901 caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta cttgcctaac
    18961 gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt
    19021 tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact tttctaaaaa
    19081 aacaaggtgt gacaaaacat atgtcacacc ttgttttttt ctttcattct cttaactatt
    19141 ggcaaaaaga gtatcccttt tctgaccgct acttgctgag cagaataaat accgctgttg
    19201 cctgaacaca taaagctgat caagcagatc ataaagaagt atactgccgc ttgtgaaccg
    19261 aacaattcga tccccatcac aaagcaggca atcggtgtat ttgtcgcacc agaaaatact
    19321 ccgataaagc ccaatcctgc aagaaaagga acagaaacat ggagcaaagg cgctagtgct
    19381 gcaccaagcg ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg
    19441 cctaaagaca aaactgtgaa gaataacttc ccaataaaat catagacatg tgcctgtcca
    19501 ttaaatgatt cctgcagtaa tggaagactc aatccgagat aacgttgtgt ttgcaaaatc
    19561 aatgctgcgg ctacaactac agccccacct atcgtatttc gccaaacagg atgaggaatc
    19621 cattgtgcat atcgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa
    19681 ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc
    19741 acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcataaa atttgcaaac
    19801 agtgcagcaa agaagctagg gaagatcgct tctgttcgga ctttcccgat tgccaacact
    19861 tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg
    19921 attccactga taatcaagat ttcccgttct gatttatcca atcggaacaa acgtgctgta
    19981 ttatctgcta acgctccccc catttggact gctgttcctt cacggccaac agatcctcca
    20041 aataaatggg tagttatggt tccaaacagt gtaagtggaa taagtcgcaa cgggattttt
    20101 tcttctccac catttccttg atcgatcact aagttgtttc cacgactaga aagaccgccg
    20161 tgatacgcat agagataagc aaaaagcgca ccactaactg gtagtagata aaggagccaa
    20221 ggatgtgata gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa agcagataag
    20281 ctccccatga atataccgag aatcgctgtg atcaccagcc acttcagcat gtacactaaa
    20341 atgcgtactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat aaaaaaaggc
    20401 tctataattt atgggactga tgaatcagaa ttgattcttc agccccattt tcattttagg
    20461 tattaaaaaa c
//
LOCUS       DAZBWR010000049        20217 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000049 DAZBWR010000000
VERSION     DAZBWR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.049"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..2130)
                     /gene="glyS"
                     /locus_tag="ACHGT5_001514"
     CDS             complement(55..2130)
                     /gene="glyS"
                     /locus_tag="ACHGT5_001514"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN7217503.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2132..3049)
                     /gene="glyQ"
                     /locus_tag="ACHGT5_001515"
     CDS             complement(2132..3049)
                     /gene="glyQ"
                     /locus_tag="ACHGT5_001515"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN7217504.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3433..4245)
                     /gene="recO"
                     /locus_tag="ACHGT5_001516"
     CDS             complement(3433..4245)
                     /gene="recO"
                     /locus_tag="ACHGT5_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN7217505.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4388..5287)
                     /gene="era"
                     /locus_tag="ACHGT5_001517"
     CDS             complement(4388..5287)
                     /gene="era"
                     /locus_tag="ACHGT5_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN7217506.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5302..5697)
                     /locus_tag="ACHGT5_001518"
     CDS             complement(5302..5697)
                     /locus_tag="ACHGT5_001518"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN7217507.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5681..6157)
                     /gene="ybeY"
                     /locus_tag="ACHGT5_001519"
     CDS             complement(5681..6157)
                     /gene="ybeY"
                     /locus_tag="ACHGT5_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN7217508.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6174..8366)
                     /locus_tag="ACHGT5_001520"
     CDS             complement(6174..8366)
                     /locus_tag="ACHGT5_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN7217509.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8390..9361)
                     /locus_tag="ACHGT5_001521"
     CDS             complement(8390..9361)
                     /locus_tag="ACHGT5_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN7217510.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10067..11446
                     /gene="brnQ"
                     /locus_tag="ACHGT5_001522"
     CDS             10067..11446
                     /gene="brnQ"
                     /locus_tag="ACHGT5_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN7217511.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11602..12048)
                     /locus_tag="ACHGT5_001523"
     CDS             complement(11602..12048)
                     /locus_tag="ACHGT5_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN7217512.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12075..12251)
                     /gene="rpsU"
                     /locus_tag="ACHGT5_001524"
     CDS             complement(12075..12251)
                     /gene="rpsU"
                     /locus_tag="ACHGT5_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN7217513.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12413..12841)
                     /locus_tag="ACHGT5_001525"
     CDS             complement(12413..12841)
                     /locus_tag="ACHGT5_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN7217514.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12933..13793
                     /locus_tag="ACHGT5_001526"
     CDS             12933..13793
                     /locus_tag="ACHGT5_001526"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN7217515.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13877..14227)
                     /locus_tag="ACHGT5_001527"
     CDS             complement(13877..14227)
                     /locus_tag="ACHGT5_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN7217516.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14243..15970)
                     /locus_tag="ACHGT5_001528"
     CDS             complement(14243..15970)
                     /locus_tag="ACHGT5_001528"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN7217517.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16183..17109)
                     /gene="trxB"
                     /locus_tag="ACHGT5_001529"
     CDS             complement(16183..17109)
                     /gene="trxB"
                     /locus_tag="ACHGT5_001529"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN7217518.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17254..18687)
                     /locus_tag="ACHGT5_001530"
     CDS             complement(17254..18687)
                     /locus_tag="ACHGT5_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN7217519.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18687..20090)
                     /locus_tag="ACHGT5_001531"
     CDS             complement(18687..20090)
                     /locus_tag="ACHGT5_001531"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN7217520.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         5441 a         4400 c         3616 g         6760 t
ORIGIN      
        1 ccagagaaat agcctatttt gtgcctgttc tctggtttat ttacatatta gaaattattt
       61 tgtgatgatt tgatctaaac ttgctagtga caaagccatg gcagcaattt gacttaattg
      121 cgttaatcgg ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata
      181 attttcgatc agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc
      241 agcaaagctt gttttcattg cctctactgc tttgtgcagt tctttctccg cttcgttttc
      301 gaacagttca gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac
      361 gacacgtgtc aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa
      421 caattgtgct gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc
      481 gatgacatca tgacggatat tcttcgttgt caataattgg cgcagacggc ctttgataaa
      541 gtcgatcacc tgttgttgtc ccgctgacaa gccaatgcca aaacgatccg tatctttact
      601 gataacttca ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc
      661 gatgattcga acgacaccat atgcttgacg gcgcaaagca tatggatcgt ttgagccact
      721 tgggatcatc ccaactgcaa agaaagaaaa gatactatct aatttatcag caatcgctaa
      781 aacagaacct actgtagtct caggtaaatc tccttcactg gatataggca tgtaatgttc
      841 acggatcgct gttgctacgg ctggtttctc gcctttcagc agagcatatt tttctcccat
      901 gatcccttgt agttcaggga attctcctac catgttggtt actagatcaa atttatagat
      961 ttcagcagcg cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgcctat
     1021 gatgcgagcg atgacttgta cccgttgcat tttctcatag atgctaccga ttttttcatg
     1081 gaaagtaaca gtttttaatt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt
     1141 ataaaagaat tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgtaccac
     1201 attttccaat tttacccgat cgccattacg agcagaaata aaatgaggaa gcaacatacc
     1261 ttgatcgttc cgtacttcga aataacgttg atgttctttc atagaagtga ctagtacttc
     1321 ttcaggaaca gatagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac
     1381 taaattgttg acttcttcta gtaaatcttc atctagatca atgatccagt gatttttttc
     1441 ggctaattcg tttgcttgtg tgatgatcat ttcttttctt ttttgcggtg aagcaataac
     1501 aaattgttca ttcaattttt cttcgtattc ttttgcatgc gtaaatgtca catcttcgcc
     1561 taagaaacga tgacctcttg aagctctgtc tgttttgaca tccaagatct caaacgggat
     1621 tatttcgtca tctaacaatg ccacgatcca gtgaataggg cgaatatatt caaaatcgta
     1681 ttctgcccag tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt
     1741 caatacttct tttgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc
     1801 ttttaattct ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc
     1861 agctgctttc gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac
     1921 ttcttcttcg ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg
     1981 cggtgtagaa aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc
     2041 tgttttttct tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag
     2101 cccgatttca agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat
     2161 ttgctgtgtt tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt
     2221 tggctacagc acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg
     2281 atactgcacc gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat
     2341 acgctggatg aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa
     2401 agttgcttag tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt
     2461 ctggttgttt gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac
     2521 tttcaacttc ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa
     2581 ctggtttaca aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc
     2641 catcaagcca tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt
     2701 cttcaacaaa acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta
     2761 aataaagctc ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct
     2821 gatacaaacg gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta
     2881 cataagcagc attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg
     2941 tacctgctcc tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa
     3001 atttttgtaa tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc
     3061 ctttcaattt ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc
     3121 ctatgtctga ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata
     3181 aagattcggc agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc
     3241 ggcttccact ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac
     3301 gtattcattt tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta
     3361 atctacggtt aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt
     3421 tgtatctttc atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg
     3481 cgtctttaat acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg
     3541 aataccgaca tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc
     3601 ttctttcaag tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa
     3661 ataaacagcg cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca
     3721 tatcacaccg ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac
     3781 acagtgcgtc caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt
     3841 cgtgatgatt tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg
     3901 gaataaatgc gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata
     3961 ggtaccataa ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt
     4021 gacactgctt aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt
     4081 aaaggggagc aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa
     4141 catcattttg ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt
     4201 acggctagat aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa
     4261 aaataaatct ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc
     4321 gtttttctca acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt
     4381 cagaatatca gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc
     4441 gccagtcttt ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt
     4501 cgatatcctt tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa
     4561 tgattccttt ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat
     4621 tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg
     4681 ttaaaaacaa gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag
     4741 tcacttgatc atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca
     4801 ttaagcgttc gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca
     4861 tctgcgtcga ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt
     4921 tattgataat cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt
     4981 ctccccggcc gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca
     5041 acgcactgta agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc
     5101 ctggtgtatc gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat
     5161 ttctagttgt ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca
     5221 ataatgtgga ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt
     5281 ctggcatata cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa
     5341 aatcaaccca atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc
     5401 tttgattttt ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc
     5461 aaaaacagta ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa
     5521 ccactctatc cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc
     5581 aacatgtttc ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa
     5641 ttccacagac atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg
     5701 catccaagat ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt
     5761 catacccgtt gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg
     5821 aatggccgta agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta
     5881 gtacacgagg caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt
     5941 cttcttccag agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat
     6001 tgattacttg gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca
     6061 gctttaaata agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg
     6121 agacttggtt tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct
     6181 tcttgttctt cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa
     6241 tggaaggtgc tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag
     6301 gtcagtccac tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca
     6361 aaactttcga ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg
     6421 tcagcaatat tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat
     6481 tgctcttctg ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc
     6541 gtggttccat gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc
     6601 aatattttgg ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
     6661 aagaaattgt gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt
     6721 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg
     6781 atttctgcta cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct
     6841 ggcgcttctt ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat
     6901 acaattacac tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc
     6961 aagaatgaga agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca
     7021 ccaaagctca ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg
     7081 acccacatgg cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc
     7141 atgagcccag agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa
     7201 tcataaaaaa tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt
     7261 cggttcaaga aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata
     7321 taagtagctt gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac
     7381 atagctgtca catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt
     7441 gaattataaa tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg
     7501 aaaatcgaag tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca
     7561 tcaatttgat ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca
     7621 gcttctcgtg cagattgttt taattcttct gtctttttct cattcaatac atcattgact
     7681 acaatgcctt gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa
     7741 tactgaatct gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt
     7801 ttactcatct gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt
     7861 tcagtcattt caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg
     7921 ttttctgcat tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta
     7981 ggtttcggga tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg
     8041 atggtatcat tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca
     8101 tctgccagat ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt
     8161 ctttgttcgg tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct
     8221 acttggcctt ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag
     8281 acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt
     8341 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
     8401 gaagatccgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
     8461 cacttgcttc aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat
     8521 gaacaagacc acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa
     8581 tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt
     8641 tttgtgcttc gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta
     8701 atggtgcgat ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa
     8761 tttggtataa agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc
     8821 cgggtagaaa gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt
     8881 gcacttcgcc tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg
     8941 ttccggctgg ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct
     9001 tttgaccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
     9061 catacatctc aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga
     9121 catctggtgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg
     9181 aggcagccaa ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat
     9241 ggatggttgt ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa
     9301 gcatgctgac atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca
     9361 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
     9421 ctatatagca aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg
     9481 ttaaaaaata gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat
     9541 gtaagttata tgaaagtgga gaacaaaata ttccgtttca ttcagaggta ttctctaggt
     9601 atttttcttg tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa
     9661 aacaggtgta ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag
     9721 gctattcaac gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata
     9781 gacgaacatc atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat
     9841 caggctagcg gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa
     9901 aaaaggtggt accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt
     9961 tatattttaa ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca
    10021 acgtttatat cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa
    10081 aacttattca tggaaacaac tattgatggt cagttcactt atcttcggga tgtttttcgg
    10141 gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag
    10201 tgctggcgta ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt
    10261 agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc
    10321 tgccttgttc ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac
    10381 agctgctact gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt
    10441 aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc
    10501 cacacggttg gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc
    10561 aatcattttt gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga
    10621 tacttaccaa acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc
    10681 agtagccttg cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa
    10741 agataaaaaa gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt
    10801 catgatctat tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc
    10861 agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc
    10921 tagcttctta ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt
    10981 ttcttttgca caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg
    11041 gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg
    11101 gtctttacca gtattgatgc ttttgtatcc attgtcgctt gcgttgattt tgctttcctt
    11161 aacagcaaaa tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc
    11221 tgttcccgcc gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt
    11281 tgtagcaagc atgctcgaat tttatcatca tcacgttcct tttgctgcat tgggacttgg
    11341 ctggatggtc ccaacgttac ttgggtatgc tggcagctta ctcttctatt acgcctatcg
    11401 cctatcaggg tataaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca
    11461 atttaacata agaaatatta aacaggaaaa aaacagctag atgaaagaat cctattcttt
    11521 cgtctagcta ttttttagat ctctttttta cgagcacaaa aaaactgcgg acaatgattg
    11581 tccgcagttt aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg
    11641 gtttccatct gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc
    11701 tttcattgat gaagcaccag tttgcgtgat tgcttcttga acaagttgtc gaatttcttc
    11761 gtcggaaagt tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc
    11821 agctaaatcg tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat
    11881 ttcgcgagac aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg
    11941 ctcgttttgt atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc
    12001 tttcatcgct gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc
    12061 aattcatcac agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt
    12121 cttacacttg gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt
    12181 gacacggaac gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact
    12241 gtttttgaca tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa
    12301 atatatctca aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc
    12361 aaggaaatct tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac
    12421 aattttcaca tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct
    12481 gttcttcgaa ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac
    12541 agataaagtg atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct
    12601 ctccatttaa atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat
    12661 aaatcgtatc gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag
    12721 cggatacgta gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat
    12781 atttaaaacc gttatttttc aatgtttcta gtgattgttc gatcaacgtc tgttttttca
    12841 tgacttattc ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata
    12901 aacataaaaa aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta
    12961 ctttttttgt catctccgac tctgctggag aaactgcgac aaaactagcg caagccacta
    13021 tggcccaata tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga
    13081 aaacattgat gcaggcactg caagatgcac tagaagaaaa agctcttgtc ctgcacacat
    13141 tgatcaatca agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta
    13201 gttttgatgt gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca
    13261 ttcggcaacc tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa
    13321 tggaatttgc tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg
    13381 tcgttttgct aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca
    13441 aaaatttaaa agttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt
    13501 gggagatcga tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata
    13561 tccgtaaaga acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg
    13621 aaaaaatacg agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag
    13681 taatcaatgt cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag
    13741 acctagaaga ccatagttat tatggaactg aaaccaccga agaaacagac taacaatttt
    13801 tctttaaaaa aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct
    13861 ctatttgcac ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca
    13921 gtacgtgaga atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga
    13981 catggttttc tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg
    14041 cttggatgat tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac
    14101 gagtggggtc actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg
    14161 tgtctggcaa tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt
    14221 tttccatcag tctcaagttt ttttattctt ctgtcatctt ttggataatc gtttcataag
    14281 cggctacttt ttcagctgcg tcttctgaag agtttccttt tacaccgata taaaatttga
    14341 ttttcggctc agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact
    14401 tcaatacgtc agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg
    14461 tcaactcttt gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt
    14521 cttcacggaa tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg
    14581 tcacagaaat cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat
    14641 catacaacgt tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg
    14701 cttggatcgc gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga
    14761 atccgaacat aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga
    14821 atttgaatcc tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag
    14881 gtaattcgct agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg
    14941 attggtgtgc ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata
    15001 cttcatagga gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg
    15061 ttgcaatcag caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag
    15121 agtgttcttc tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt
    15181 ctggaactag tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt
    15241 ttcctgttcc gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca
    15301 attcatgatc aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc
    15361 cgatgatcgt gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta
    15421 acggattgct gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt
    15481 gtcccccatc ttctccgtag actttatatc cgttgtaagc tgctggattg tgggatgcgg
    15541 ttaccatgat tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg
    15601 gacgtagact ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt
    15661 ccatggcaaa ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt
    15721 ttgtttcttc gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg
    15781 tatagatgtt cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga
    15841 attccaacgg tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta
    15901 attgtttttt taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt
    15961 cccaagacat gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca
    16021 cttattagat atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta
    16081 tcaagcacgg ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa
    16141 gagtaaaaga acaattaaac cgttcttcca ctctttttaa acttatgcag ttgtttcttc
    16201 taaagattcc aagtatttat acacttgttg tccagcgatc ccaccatcac caacagcagt
    16261 agtgatttgt cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggtatatt
    16321 cgtcttcatt tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct
    16381 aaatggtgca gttaatggat ccagtccaac atagatgaat actccgtctg ctgccaaatc
    16441 aaactcattg ccggttttga cattacgtgc tttcacacct gtcactacca tttcattccc
    16501 aacgatttcg tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg
    16561 gtcttggatg atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga
    16621 agcaaattgt gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac
    16681 tagacgtttg ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc
    16741 agcaaattct tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat
    16801 gactgttttt ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc
    16861 cttgatatct tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata
    16921 catcttataa gctagatcag gacccattat tgaatcgaaa ccaggatagt tttcgatttc
    16981 tgccgtattg ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt
    17041 tgaacgtgaa gcatataaag cagcagtcat cccagcagga cctgcgccaa taacgattac
    17101 atcatacata tgtagattct cctttactta atcaagtaag catactttac agccaatgcg
    17161 aaaaaaagtc tattgttctg cttgcgtttc tcgaatagct ttattatact cgaaaaatcc
    17221 gctgaaaaac aatagacttt tataaatcat tgatcaatca ttcgaaagat tttctccatt
    17281 tgaatcaatt acttttttat accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc
    17341 atttccttcg ttgtcacgat cgacataaat gaatccgtac cttttcttca tctcacctgt
    17401 accagctgat actaaatcaa tacatcccca agtcgtatag cctaatagat cgactccatc
    17461 taattctact gcatctttca ttgcctggat atgtgctgcc agatagtcga ttcgatagtc
    17521 atcttccaca taaccattct catcgggcgt atcgactgca cctaagccgt tttctacaat
    17581 aaatagtggt ttttgataac gatcataaat atcattcatc gttattctca agcctaatgg
    17641 atcaatttgc caaccccatt cacttgcttc cagatatgga tttttgacag aagcaaagat
    17701 atttcctgca gtctgttcca agagttcgtt gtcagtagtc gaaactcgag aagaataata
    17761 agaaaaggag ataaaatcaa cagtatgttc tttcagcaat gcggcatccc caggttccat
    17821 tttgatctga atgccatttc gttccatttc tttcaatgca taagcaggat attctcctcg
    17881 tgactggaca tcaatgaaga aataattttc tcgatctgat tttctagaag cccaaacatc
    17941 ttcaggattg caagtgtatg gataatatgc tcctgctgcc agcatacagc ctactttgtt
    18001 ctccggatct acttcatgag caatcttcgt tgcgatggca cttgcaacca actcatgatg
    18061 tgccgcttga tatttgattt gttcttcatt ttctccttct tcaaaataca gccctgcgcc
    18121 catgaatggt gcatgaagga tcatattgat ctcattgaat gtcaaccaat atttgacaag
    18181 tcctttatag cgattgaaga tgactcggca cagattttca taaaaaccaa cgagttctct
    18241 ggaacgccac gcaccatatt ctttgatcaa atgcatcgga caatcaaaat gtgtaatcgt
    18301 cacaagcggc tcgatcccgt acttttgaca ttctttaaag acatcctcat aaaatttcaa
    18361 accagattca tttggctctt tttcatcgcc cattggaaaa atacgactcc aagcaatgga
    18421 catccgataa gtcttgaacc ccatttctgc aaataaagca atatcttcct tataatgatg
    18481 atacatatcg atcgcaattt ttgctggata aaaatgattt tcatcaaatt caaacatttt
    18541 cttttttcca gtaatgacag gaaaacggtc ttctccgatt ggcacaacat ctacgttcgc
    18601 taacccacgt ccgccttcat cataaccgcc ctcgcattga ttggctgctg tagctccgcc
    18661 ccataaaaaa tcttttctga atcccatcat acttcctcca ttacatgtgt attgcttgat
    18721 aattctcttg ccagttcatc ttggacttcg attccttctg cttttaaccg gctgattgtt
    18781 tccttgaatt gcggtaaata ctgctgatgt gcaataaaca actcattcat gatccgtttt
    18841 gctgtctttc ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc tgttccataa
    18901 tcaccagtca cagctgcttc gatcgtcagc aattccatat tcttcattac ttgcaaccag
    18961 cctctttcag cagcaggaag ggaaccaaac gctacatttc gtgcgccttg tgcgccaaca
    19021 taggcagtga cttctactac acaatctgcc ggcagatcag gaacagctcc atcattcttt
    19081 gtagatacca cgatctgtgt ttctttattt ccatagatcg aagcaatcgt ttcacaggca
    19141 gcatctgaat aataagcacc acctcgtttt tgcaattgtt caggtttatg atccaattct
    19201 ggatctttgt ataattcaaa cagctctgct tctgtttctt tcacttgctg cgctcttgta
    19261 ccaatcgttt gatattcttc taaagcatgt tccagcattt cttcttcacg gtagtaatag
    19321 cggtggtagc cacaagggat catgttcatt tgttttaatt gttctttgaa aaacgggata
    19381 tcaaagatat ttttcggcaa tccattgtct tcatcgtata aacgatcaat caattctgcc
    19441 gtgcggtcat ttcctttatt atctgctact ttgtgccaat ggaagtgatt tagaccggca
    19501 aacttgtaga tcagctcttc ttttttcttg ccaagcattg ttggttctgt catcatcgcc
    19561 attactggca cattgcataa tcctatcact ttatcccatt tgccatatct gataactgct
    19621 tctgtcacca tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc
    19681 ttcatgtctt caacgattgc caaaaccact ggaacggtac gaaaagcttt gaatatcccg
    19741 ccagcaccat ttgtttcttg cccaatcatc ccataataac tagggatccg ctcgtctttg
    19801 attcgtgcat ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc atctttcaat
    19861 gcttcttccc gatccagagt tgcatagatt ttgacttcat acggagaagc atcccacata
    19921 cgctgagaca tctgactgac gatctcaagc ttttcttttc cttcttctac atctacgagc
    19981 cagatttctc gtatcggtaa ttcctcatat cttttgataa atccttccat caactcgggg
    20041 gtgtaactgc tgcctccacc aatcgtagcg attttgattc cttttgccat gttctcactc
    20101 ctttactttt tacactttta ttatcttttt tgtaaacaaa acaaacaata gaaattcgtt
    20161 tctttccagt aacatttaca aaagttagtg ttttctactg gatttttgta aatatct
//
LOCUS       DAZBWR010000050        20181 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000050 DAZBWR010000000
VERSION     DAZBWR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.050"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACHGT5_001532"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACHGT5_001532"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN7217521.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACHGT5_001533"
     CDS             1011..2258
                     /locus_tag="ACHGT5_001533"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN7217522.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACHGT5_001534"
     CDS             2334..2678
                     /locus_tag="ACHGT5_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217523.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACHGT5_001535"
     CDS             complement(2795..3121)
                     /locus_tag="ACHGT5_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217524.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="ACHGT5_001536"
     CDS             complement(3494..4696)
                     /locus_tag="ACHGT5_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7217525.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="ACHGT5_001537"
     CDS             4813..5328
                     /locus_tag="ACHGT5_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN7217526.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="ACHGT5_001538"
     CDS             complement(5527..5973)
                     /locus_tag="ACHGT5_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN7217527.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACHGT5_001539"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACHGT5_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN7217528.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="ACHGT5_001540"
     CDS             complement(7852..8481)
                     /locus_tag="ACHGT5_001540"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN7217529.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="ACHGT5_001541"
     CDS             complement(8465..8929)
                     /locus_tag="ACHGT5_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN7217530.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="ACHGT5_001542"
     CDS             complement(9416..10684)
                     /locus_tag="ACHGT5_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN7217531.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="ACHGT5_001543"
     CDS             complement(10915..11652)
                     /locus_tag="ACHGT5_001543"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN7217532.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="ACHGT5_001544"
     CDS             complement(11719..12288)
                     /locus_tag="ACHGT5_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN7217533.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="ACHGT5_001545"
     CDS             12416..13288
                     /locus_tag="ACHGT5_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN7217534.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="ACHGT5_001546"
     CDS             13630..14502
                     /locus_tag="ACHGT5_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN7217535.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /gene="nagE"
                     /locus_tag="ACHGT5_001547"
     CDS             14499..16457
                     /gene="nagE"
                     /locus_tag="ACHGT5_001547"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN7217536.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="ACHGT5_001548"
     CDS             complement(16708..17559)
                     /locus_tag="ACHGT5_001548"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN7217537.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="ACHGT5_001549"
     CDS             17682..18149
                     /locus_tag="ACHGT5_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN7217538.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="ACHGT5_001550"
     CDS             complement(18412..18894)
                     /locus_tag="ACHGT5_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN7217539.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="ACHGT5_001551"
     CDS             complement(18994..19857)
                     /locus_tag="ACHGT5_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN7217540.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3662 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgactccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DAZBWR010000051        19508 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000051 DAZBWR010000000
VERSION     DAZBWR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.051"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHGT5_001552"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHGT5_001552"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN7217541.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACHGT5_001553"
     CDS             complement(1173..1868)
                     /locus_tag="ACHGT5_001553"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN7217542.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACHGT5_001554"
     CDS             complement(1881..2597)
                     /locus_tag="ACHGT5_001554"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN7217543.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACHGT5_001555"
     CDS             2927..3532
                     /locus_tag="ACHGT5_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN7217544.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACHGT5_001556"
     CDS             3857..4180
                     /locus_tag="ACHGT5_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN7217545.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACHGT5_001557"
     CDS             4362..5732
                     /locus_tag="ACHGT5_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN7217546.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACHGT5_001558"
     CDS             complement(5862..7163)
                     /locus_tag="ACHGT5_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN7217547.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACHGT5_001559"
     CDS             complement(7393..9384)
                     /locus_tag="ACHGT5_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN7217548.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACHGT5_001560"
     CDS             complement(9528..9794)
                     /locus_tag="ACHGT5_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN7217549.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHGT5_001561"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHGT5_001561"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN7217550.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHGT5_001562"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHGT5_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN7217551.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACHGT5_001563"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACHGT5_001563"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN7217552.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACHGT5_001564"
     CDS             complement(13954..14475)
                     /locus_tag="ACHGT5_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN7217553.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACHGT5_001565"
     CDS             complement(14478..15320)
                     /locus_tag="ACHGT5_001565"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN7217554.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACHGT5_001566"
     CDS             complement(15313..16038)
                     /locus_tag="ACHGT5_001566"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN7217555.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACHGT5_001567"
     CDS             16214..16510
                     /locus_tag="ACHGT5_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217556.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACHGT5_001568"
     CDS             17078..18670
                     /locus_tag="ACHGT5_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN7217557.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACHGT5_001569"
     CDS             18683..19423
                     /locus_tag="ACHGT5_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN7217558.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5581 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaa
//
LOCUS       DAZBWR010000052        19100 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000052 DAZBWR010000000
VERSION     DAZBWR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.052"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..781)
                     /locus_tag="ACHGT5_001570"
     CDS             complement(56..781)
                     /locus_tag="ACHGT5_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN7217559.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(1142..1212)
                     /locus_tag="ACHGT5_001571"
     tRNA            complement(1142..1212)
                     /locus_tag="ACHGT5_001571"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Gly,seq:ccc)
     gene            1329..2081
                     /locus_tag="ACHGT5_001572"
     CDS             1329..2081
                     /locus_tag="ACHGT5_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN7217560.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2097..2576
                     /locus_tag="ACHGT5_001573"
     CDS             2097..2576
                     /locus_tag="ACHGT5_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN7217561.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2827..4407)
                     /locus_tag="ACHGT5_001574"
     CDS             complement(2827..4407)
                     /locus_tag="ACHGT5_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN7217562.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(4400..5143)
                     /locus_tag="ACHGT5_001575"
     CDS             complement(4400..5143)
                     /locus_tag="ACHGT5_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217563.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(5319..6095)
                     /locus_tag="ACHGT5_001576"
     CDS             complement(5319..6095)
                     /locus_tag="ACHGT5_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN7217564.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(6343..7179)
                     /locus_tag="ACHGT5_001577"
     CDS             complement(6343..7179)
                     /locus_tag="ACHGT5_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN7217565.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(7190..8119)
                     /locus_tag="ACHGT5_001578"
     CDS             complement(7190..8119)
                     /locus_tag="ACHGT5_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN7217566.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            8311..8664
                     /locus_tag="ACHGT5_001579"
     CDS             8311..8664
                     /locus_tag="ACHGT5_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN7217567.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(8738..9394)
                     /locus_tag="ACHGT5_001580"
     CDS             complement(8738..9394)
                     /locus_tag="ACHGT5_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN7217568.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            9620..11554
                     /locus_tag="ACHGT5_001581"
     CDS             9620..11554
                     /locus_tag="ACHGT5_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN7217569.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(11640..12863)
                     /locus_tag="ACHGT5_001582"
     CDS             complement(11640..12863)
                     /locus_tag="ACHGT5_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN7217570.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(12857..14728)
                     /locus_tag="ACHGT5_001583"
     CDS             complement(12857..14728)
                     /locus_tag="ACHGT5_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN7217571.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(14749..15867)
                     /locus_tag="ACHGT5_001584"
     CDS             complement(14749..15867)
                     /locus_tag="ACHGT5_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN7217572.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(15950..16783)
                     /locus_tag="ACHGT5_001585"
     CDS             complement(15950..16783)
                     /locus_tag="ACHGT5_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217573.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(16776..17693)
                     /locus_tag="ACHGT5_001586"
     CDS             complement(16776..17693)
                     /locus_tag="ACHGT5_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217574.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(17920..19044)
                     /locus_tag="ACHGT5_001587"
     CDS             complement(17920..19044)
                     /locus_tag="ACHGT5_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN7217575.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5949 a         3767 c         3191 g         6193 t
ORIGIN      
        1 gtatgttttt acacaatttt ttcataatat tcataaaaaa ttcgaatagc tgctactaat
       61 tttttgtttt tgttgcttgt cctgtctcat aattgatcgt gtagccatct tgaacattgt
      121 agataaagac attgaattca atttttttgt cttcaatgct ttctgcttcc aattgcactc
      181 ccctggctac cagttcattc ccctcaaaat gaggagtgac acggtagcgt acatgatgat
      241 ttgtttcttt gatatatgcc gcaatatcat tttcgtgagc aagcatgtat ggagcattca
      301 acgaacgtgt ccctgtcatc aagtttttaa tgttgttatt ttgaccagtc agctggaaac
      361 caattaaatg gctgcgatta tataaccatt gcccgtcact taatttcttt tgtttccaac
      421 ctgtcgggtc aatatacaaa ggctctctct tttcagtagg gaacaaatct tttccaagca
      481 tagcattggc ttgtcctact ctgttcaatt tatcaatatt tgaatagctt tcccatgttc
      541 ctttggaaag gctaagatcc tcttttgtaa attccggctg attcccattg atttctattt
      601 cttgatgttt tccatcatat tctggcacat ccgctacttg agaagcttgt ggtgtggaat
      661 caaaaatagt tgtaatctgc tgaaagatat ctgaatcttt ttgtgtggtg gaaacaccga
      721 atgcccctgc aatcacagca gcgacgatcc ccacgatcgc cttggttgtt tttttgctca
      781 tttctttact cctttactaa cagctcccta aatcttaaca tatgttcccc tataaataaa
      841 tactagaaag aaatagaaac agcaaaaaat aaaacctgtt ttaagatggc ttaaaaatac
      901 aaaaaagaga cggattcctc gtctctaatc agtcaccact ctatctgtaa ggtatttatt
      961 attattcaaa aattttcttc atactcaaga caatacactg atttcacaac cgcttggtaa
     1021 acggtgcgca aaagcttgca tctgcggtaa taagtccatc caagttaaaa acataagaag
     1081 ttgtttttgc ttggctgttc ttattacttg ttgcagagca gctttttcac aacctatttt
     1141 aagcgggtga cgggaatcga acccgcgaca tcagcttggg aagctgggat tttaccacta
     1201 aactacaccc gcaatacaaa aacagtatac gcctctttta ttccttcctc aagttttttt
     1261 attctttacg cttctggtat gttttgtagt aaaataaaac taatcaatca attattggag
     1321 aagatgctat gacgggaaaa gaattagatc tgttgctgga cacttgttta ctcgctggga
     1381 aaatcatgat ggaaagcaat gcggagatgt atcgggtaga agatacgatg agtcgaattg
     1441 ctttagcttc tggaaatttc cgtttggtca gctatgtcac tcaaaccggt ctctttattg
     1501 gtcttgatcg tacctcgacc atccgaatgg aacaaattac caaccgttcg attaatttag
     1561 aaaaagtagt aaaagtaaat aatctttcac gtaaatatgt tgcttcagag ctaacattag
     1621 aagaacttta ctgcgaactg caagcaatcg accgagaccg ttctttcttt cctgtctggt
     1681 tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat ggtgttattt ggcggaaaga
     1741 tgaccgattt acctgtgact ttagtcattg gaggattggg atacgcagct tatctatata
     1801 gtttgaaatt ttttagaatc aaatttttat cggaattttt atcttcttta ttgattggca
     1861 tagccgctat ctacagcgta cgttttggct taggaagcaa tcaggatcta atcattatcg
     1921 gatgtgtgat gcctttagtt cctggagttc aaattacgaa cgctatccgt gatttgctag
     1981 ctggacatta tgtctcagga gtttctcgcg gaacagaagc gatgatgact gctacgatga
     2041 tcggttttgc gattgctttt attttccagc ttttttatta ggaaaggaac acaagtatgg
     2101 ggacgatatt tattcagttt tcttttagtt ttttagcgac tgcggcattt gccgttatta
     2161 caaatgtacc taggcgttca ttgatttgtt gtggtctttc tggtgctttt ggctggatga
     2221 tctactggat ctgtgttcaa ataggcggat ctgctgcttt tggttcattg cttggttctt
     2281 taggagtagc attcatcagc gatcttttct ctaaacgttt gaaaatgccc gtaacgattt
     2341 tcaatattcc cgggatggtt cctctagtcc ctggtggttt agcttatcaa gcggttcgaa
     2401 atctcgttac tgaatcctat cgagaagcgg ctcattatgc ggtccaagca attatgattg
     2461 ctggagcaat cgctttaggt cttgtattat cagaagtctt taaccataac atccgtaatt
     2521 tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa acggcttttt aaataaacga
     2581 agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct tgagcgatga caaaatatct
     2641 cacaaacatt gcgacaagta accgcaggag caataagaag taattatgag tattaaaaaa
     2701 agttttgtaa aaatagcttc tcacattttt acataaaatt gacttaatac tgagaaactg
     2761 acttttgaac accctctatc tgtaaaaaag gggctgcgac atgatttttg tcacggcccc
     2821 tcttctttat tcaaattttt tccaaaacgt tcgttgataa taaacgatcc atagagcgga
     2881 aagtattaga actaaaccga tcacaggaat attcactaac caaaatggat aaaacaaagc
     2941 caacattccg tataagacga taagaataat actgatgatc aatgaaccca tcatagttgc
     3001 gactaaaccc aaacttcctg aacctctgtt aaataattgg ttaatattgg tccagttcag
     3061 cagcagcaac cgataatctc tagcgaaata acgtagtgac aataagtagc agccgaataa
     3121 agaaccaaat agcaagctag ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa
     3181 taatgcgatc ccaccagtta aaaaactctg caatatccat ccgaatctga acttttcttt
     3241 taaatattgg ttcatagaga ttggcaaaga acggataaac agaaaatttt cttgatccaa
     3301 agaaataaga ttgctgataa aagaagtctg attaactgtt aagaaagcca atgcgatccc
     3361 taccaggaaa acaacaccga ttaacttatt atctaaatca gttaatttta tttcgcctcc
     3421 tagggcaaaa gcaagaataa aaatgattgg agttaacaag gaagaagaaa gaacttgcat
     3481 aatcaaatta ggttctttta ctaattgagc attgtaacta aataaaagct gatgcacatt
     3541 ttggttcttt ttatgggttc tttttatttt gaactcactg tttgaatcag tcagttgctc
     3601 tgtaagcttt ggtactatca gctgttttac agcaaggaaa aatcccagag ccgtgatagc
     3661 tagtattccc caacccaata aaccttgttg agaaagtggc tgactggcag catcatagaa
     3721 tggcaataat atcgaaatcg gatgccgatc tgttaactca ttttcaatag tccccgtttg
     3781 atgattcatc cacatgattc cagcaaatgc gatcagcatc gagctgccaa gcaagatact
     3841 agaaactgtt tttctatgtt ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa
     3901 actagccaga caaaaaagaa taatcaaaaa tagagaaaac aaaaggattg agataatgat
     3961 agtaagcaaa atccctagat cagctcgcca tccggttaaa ataaacatga ctagaagcgg
     4021 aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc
     4081 ttgtccaaaa ggcagaggca aaaatacttg caggtcattt ccttcaaaaa agatattata
     4141 aatgactgat atactttgag ttaaacctaa cataccaaaa agtgccacgt aataagtaaa
     4201 gaaacctggc aatttaacaa agttcatagc aaacatagca aatccgtaaa tcagtaaaaa
     4261 aatcagccct gaaaaaagat tttgagacaa aagatagata gttaatcgct ttcccttttt
     4321 tcctttctct cttgctttat tcgtgagctg tggattaaca tataaaagat taactcgaat
     4381 taattctaat aattgtcgct tatacatacg gtccatctcc tgtcgcttgc cttccagcca
     4441 ttttcaaata aatcttttct agcgtttcat caggtgagcc attcaataat tcactaactg
     4501 ccccctgata aatcaattca cctttcttca aaatagccag tttatcgcat aactgttgtg
     4561 cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc ttttgcggca tgagccttca
     4621 tcatttcttt caaatcaaat gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata
     4681 cccaaatatc tggatctggc agcaacgtgc cgatcaaaat cgttttttga cgcattccat
     4741 gtgaaaaact tgcgattgtt tcattttgat gactatacat atcaaaaagc gctgtcaatt
     4801 ctgctaaacg cttttctttt ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc
     4861 aatattctcc agccgtcaat tgtaaaaaaa tatctgggga atcaggcaca taaccgattt
     4921 tttgtttgat actcaatcga ttttccgtca atttcattcc atctactaaa atcgttcccg
     4981 atgatggttc aataatacta actaaacttt taatcgtagt tgattttccg gcaccattat
     5041 gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag agacaagtct tttaatgctt
     5101 gttttttccc gtaactttta cttacattga tgaattcaat catttctgta ctccttttgt
     5161 ttgttattac tttttattgt acccttcgtt attggtggag taaatgtcaa aaagagtgca
     5221 taacaaaaaa ggatagaaac gactaggctg ctacttgggt tgttctcttc tacaggaaaa
     5281 cactcttcta acaggtaaga tatgagtctt tgttttcttt aaaatgattc attttctatc
     5341 ttttcttgca taaaattctt tagtttatca atacttctct ttcccatctc tagttgttct
     5401 ccatttgcaa atgttactat ttcgtttact ctgctaatag atgtaatgtt ttcagtatta
     5461 atgatataag cttttaaatc tttacataaa tactcatatt cgagtgattt tttatatttt
     5521 ttaattgtgc cgtttacgag aatctcttca tctttcaata ctaggcctat tttattttta
     5581 taacctggca tgctcattag ataaaggatt tcatcgtaat ggatatagtg atcgctatta
     5641 ccataacgga cagttgtcca cctttttttc gtagcatgtt gaacaggata gttttctttt
     5701 tctttcaata agtaactttg gatgcttttt ttattttttt cataagtgaa ttcttttatc
     5761 aaataaccac taggcgtaat ctttcgatta ataacagtta ttgctttatt cggatgagac
     5821 gttagataaa caattttaca cttttcattc ttttctctaa gcatttcgcc tagttcgata
     5881 ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa tatctgagga atagatttca
     5941 ttatttttaa tgaattcaac aaattgtata acgttcaatc cctgaacgat ctcaaatttc
     6001 caaggactta ataaaattct ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc
     6061 aaactatcat ccaaaataaa tatacgcgtt accataaaaa gtacctccac tcgatatttt
     6121 actatcaaaa tcatttcata caacttttca acatacatgt taggaataaa ttgtaattct
     6181 cttcacaatc ttttccaaca cctttgaaac cgatatcaaa tactttcagg atgctgcttc
     6241 taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct
     6301 tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc ttttaaaaag aacgatacac
     6361 atactgattt ttcggagcag agactttctc agcgtttgta acaactacag atgggatttc
     6421 acgcttgaat ggttgataat agtctgattg gatggttcct tctattttat accattcatc
     6481 atttttaacc gttactcctt ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc
     6541 cacacaatgg ataatgccga atcggaacaa gaagatatct gtattgctat cgttcggtgt
     6601 ttggtaaaca aatccttcat agctgatttt tttgccaatg aattcgctag ggtaattata
     6661 aattagctcc ataacttcta aatagttttc atctgtgatc ttgatagttt gcttatcccc
     6721 atacttttct ttcaactcat tcatttggtc taagtagtct gatttgttga aataaatact
     6781 ggtatccggc tttaagtatt gtgtctgcat ctccggatcc cctactgatt ctttacttac
     6841 tgggaaatta aatcctttgg cttctacgat cgtagtgtcg agactaactg ttggaaacaa
     6901 cgtccccacg attactggca aagctagcag tacataagcg attccacgtt gataaggttt
     6961 tgacaaccca tggtcatgat cgtgctcgtg tgccttttct tctgcttttc cgtttttgac
     7021 ccacaaatac agctgaacta aagccaaaag agcagataaa atcattgaaa gaatagctaa
     7081 gtagcgataa tggacattga tatattgatc gagtttgcca gaaacttgca agtacatcat
     7141 caattcgaaa tacccagata aaatcaaaaa ccgcagcata ttttccctcc taaataaata
     7201 atgtgaatcc aataacgcta agggcaatca gtccgatcag cgataaaata aagcgtcctg
     7261 taaaatatcg tttcatcatc agtagatttt tgatatcgac cattgggcca aagaccaaaa
     7321 aggcagctac aggtgctgtg ccgaacagac ttaacaaaga agatcctata aacgcatctg
     7381 cttctgagca aagagacatc gtaaaggcca ataacagcat gatgataatg gctaaaatct
     7441 ttgttgagcc gagcgttagc ataattctag ttggtaaata agtctgcatc gcagctgcga
     7501 ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca
     7561 acacatgccc agtttgatgg atcagctgat tttttggttc attcttctca ctttgttctt
     7621 cttcatggac atgatgatgg tgattttctg ctactttaga cgtgattttt tctttcaata
     7681 cagcgtcttt ttgaaaatag gcaagccaga caccaatcac aagggcaaca aaaaagctgc
     7741 ctatcacccg ccagcttgcc atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa
     7801 tcacgattgg attgatgatc ggagcagtca gcataaaagc aaacgctgtg tgaactggta
     7861 cgcctttttt aacaaattga tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga
     7921 aaaatcctaa gatcgaacca gtaaaaatag agaagatacg attttcagga agccatctct
     7981 tcacacgttc tggggtcaaa aatacttgta aggcgcctga gataagacag cctaataata
     8041 cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt actcatctgc aaaaccgaat
     8101 ttggaataaa agaaaacatg agtttcctcc tttttatttc tagttcattc gctttgcaaa
     8161 tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat ttttacacaa ctcttatttt
     8221 ttaccaaagc acttcaattg actttttctg ttattcccat taaattaagg tctcatacat
     8281 atcacttcta tcataaaagg gagaataaaa atgaaacaaa ccactaactt gactgaagtt
     8341 caagacatac tgcaacagtc atccgttgcc gtcatttatc tttcgatgcc taattgcagt
     8401 gtttgtcacg ctgttcgtcc acgactagag gaacttctca cacattatga tattccggca
     8461 cttcatttgg atgcatttga aacaccagaa gtagctagta aatttgaagt gctgactgcg
     8521 ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac
     8581 tttaaaaaaa tccgttatct actagatgaa ctgacagcag aagatgattc cctttcttat
     8641 gaagaaatct tccgaacaaa ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg
     8701 aaagttttgc cttcatttgt tcaatcgctt tttattttta ttctgctgta ttttcggaat
     8761 gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg caattcattc gtcaaatcaa
     8821 cattttggat caatacatct gacggagcat tcaatcgagc agcggtaaag ttcaagattc
     8881 ctttgattcc tgcttccgtt aactgattga ctacttcttg tgctctttca gcgggtatcg
     8941 ttaaaattgc tacttcgatt tgttggatct tgatttgttc tgacatatct tccattggat
     9001 aaacggggat accgtctacg atgcgcccaa cgatttcctc attcacatca aaagcacaac
     9061 tgatacgtat gctattactt tgatggaatt tgtattttag taaagcactt cccaagttcc
     9121 ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt
     9181 gcataagatc ttctacatca taaccatacc cacgttttcc taattcgcca aaataagaaa
     9241 aatctcgacg gatcgttgca ctatctactt gaacggcttc acttaattca gttgaagaca
     9301 cttttttctt tccagcatta tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt
     9361 tagctgtcgc ctttggaata atatgatctt tcacgaaatg acctccacgt attgtctaat
     9421 tacatctttg atttctccat catagcagta tttataggaa aaaatcaatt ccattgctca
     9481 gatattgtga aattttattc tttattcatt tatttatcat ttctgcaaat ctcgcagtat
     9541 aatggaattt atggtagact actaattgaa aaatagtttt ttatttgagg agaattcaca
     9601 aagtagaaaa gaggattcca tgatattatt acaagccaat cagattgctc gccattttgg
     9661 tgcagaagtc ttatttgaaa acatacattt agaaatcagt acaggcgctc gtgtcgcttt
     9721 agttggcaga aatggcgctg gaaaatcgac tttattaaag atcattgctg gaattgaagc
     9781 acctgatgaa ggaaagatcg ctaaaaataa aacagcgact ctcggatacc tcgctcagga
     9841 cacaggactt gattccaatg aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt
     9901 tagacaaatg gaagagcgga tgcgcgagct agagattttc attagcgaag gtacgccaga
     9961 cacacctgct tatagttctt atttaaaaga atacgaccgg ctgcaacacg atttttcaga
    10021 tcgaaacggc tatggttatg aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga
    10081 ttcttcattt tatgatcaga aaatcgatac tttatcaggc ggacagcgca cacgtttagc
    10141 tttagctcgc atgcttttgc aacaccctga cattttgatt ctagatgaac caacgaacca
    10201 tttagatatc gagacacttt cctggcttga gaattatctg caaagctata ctggtgcatt
    10261 attgattgtt tcccatgacc gttatttttt agataaagta gtcaatgaag tctatgaatt
    10321 aagcagaaga aaaatgcgcc attacaaagg taactattcg aaatatctag atttgaaagc
    10381 agaacagtta gcaagtgaat ggaaggctta tgaaaagcag caaacagaaa tccataaatt
    10441 agaagacttc gttgctcgga accttgttcg cgcctctacc acaaaacgag cgcaaagccg
    10501 cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct caaggggatg aaaaacaagc
    10561 aaactttcta ttcagtatag aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc
    10621 cgctatcgga tatgataatg tcaccttatc tgaaccaatc aatctagata tccgtcgtca
    10681 agaagcgatt gctctagtag gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat
    10741 cattcaccag atccctttta tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg
    10801 ctattacgat caaggtcaag ctgatctgca tacaaacaag acgatcctag cagaattgtg
    10861 ggatgagcat cctaccactc cagaaaaaga gatccgaagt gctttaggtg ctttcttatt
    10921 ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt
    10981 tgctttagcc aagttagcga tgaacaaaga aaattttttg attttggatg aaccaacaaa
    11041 ccatttagat attgacaaca aagaagtatt agaaaatgca ttgatcgatt atgacggcac
    11101 actgcttttt gtttcccatg accgatattt catcaatcgg atcgcgacaa aagtcgtcga
    11161 actctctgaa aatggcagca aagtctatct aggagactat gattattatt tagagaaaaa
    11221 gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat gagccagaat ctgctcctgc
    11281 tcccacgaac aaatctgatt attaccaaag caaagaacag caaaagctta tccgtagcct
    11341 tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca
    11401 gttggaacaa gaaatggtcg cgccagaaaa cctggaagat cacgtaaaac tcaatgagct
    11461 gaaccaagat ttagaagctg ctagacaaga tcaagaaaca aaattgacgg aatgggaaga
    11521 actaagcttg gaattagaag aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc
    11581 atgctgtttt ggtttttctt tttttatttt atcaaacaaa aattccatat ttaccttttt
    11641 caccaataag aagaccaatt actagtcatt ctcactaaag cttctaaata atagtaatca
    11701 ccccaaatac aaggtgtatt taccccatta tttttgggct tattataaac agactgtgtt
    11761 aaaaagccat cttcttcttc cgttgtagca tatctttctt tcaattgtcg cataattttc
    11821 caaacagcag actggtataa gttcttttct gttttgtcta atagacctga ttctattaat
    11881 tctaataatc cacaaaccat gatcaccata ctagatgtat ctttttcttc tctgctagaa
    11941 tcatcaaaga tcaaatccca ataacaaatg gtatcttctg gcaaccgatt cataaaataa
    12001 ttggctaata ttttgcttaa atcaaaatat ttttgttctt tcaaataacg ataacttaat
    12061 gcaaaaccgt aaatccccca tgcttggcct ctagcccaac aggaattatc actataacct
    12121 tgatgcgtac ttccatatct tggttttcca tctttgacat ccatataaaa tgtatgataa
    12181 gttgaaccat cttctcttac taaataatct gctgcatttt gaatatgagc ctgagcagct
    12241 tttttatatt tttgatttcc agtttcttca ctcgcccaat acaataatgg aagattcatg
    12301 caacaatcca tgatcattct accttgttgt gaaggatcac taggatctcc ccaagcttgt
    12361 ataactcctg ccttctccca aaaacgctga agtaatgttt ctgcagcatc aattgaagct
    12421 tccttcattt ttcgatttcc cgttatttta taatctgcta cggttgataa agaatacaaa
    12481 aaaccaagat cgtgatgatt agtgataatt tgacagtcaa tcctgtggat aaaatctata
    12541 tctgcttttt cagctgtttg ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa
    12601 ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct
    12661 tttataggac actcgcttgg gaattgatac tgactgttac tgatacgttt gattaacctc
    12721 tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt ctttttccat taattcttta
    12781 taacattcat ttatccattt ttctggatgt attatctctt caaacatcac tttatcaagc
    12841 atttccccac cagcctttag cataatttta gtcttttttt attgttttta ttttggatag
    12901 ttagcccggt tgtcccatca aaatcaattt tgggacgatt gttccatact tctaaactat
    12961 aatcccgatc ttgatgagca accactcctg ttccgagcca atgggttcct tttccaaatg
    13021 taccctctaa tccaggaata aaagtgtttt cccatgtcat caagtttgta ttgggcaaac
    13081 ccataatagc tgtggatttt cgtttaatag tatcgtcact taaacagata atacctgaaa
    13141 agccagcttc actcacccta tagttttcct tgtcttcttc tatgattatt ccttctttct
    13201 caggagaaca ttcaatagca aaccctgtct catatagaac atattctgta tctaattcta
    13261 ctttgtgtac gcgaatctgc cagccttcaa aaggaataag ccaagttctg atttttgcct
    13321 gtaaagtagg ttcccatgta gaggcaagta cctctttctt acatacaatg tctcgtacat
    13381 tatttcgaac gatgaactgt ccataggttt tgatgctctc attcttaaaa ctggtcgtga
    13441 tatatgtgtc cgctcgctga atccctagca ttgaatccat tgccatttct gctttcaatt
    13501 gattcgctcg cggtatcgaa aaaccaaatt gactagaata agcaaatttt aaatattttt
    13561 cattggtcaa tgtatggaaa tttggcccta attgtcccgc attcaaaaaa aatgtatgtc
    13621 cattatcatg ttgtatgagc aaattggctt ctgaaagaag tttagttcct tcacgaatag
    13681 gcataggcat ttctttagcc gtccaatact catggtcttc aggtagcgcc aacaataaaa
    13741 aaatcttatt cagccataat ggagataacg tactattata gggttcagtc aatgtcaatt
    13801 gagggtaggt ataaccaata ttcaggatac ctttttcatc aaatatttct ttttgcatcc
    13861 accatcttat attccgattg actaacccct tgatttcacc taatttaaaa ggcaaaagat
    13921 cggcccacaa catagctgac caaaagcttg aaactgcaaa acgataaatc atgcttcgac
    13981 caaaaggcac atttgcacca tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa
    14041 tagctcggtt cttatatatc tggctaatgg gcttttctgg ttctagtacg ctataaatca
    14101 aaccataata atgaaatgca aaaggattat aataatcttg gcgacctctt gaactgtctc
    14161 gataccagcc attttgaaga taacaatttt ctatattatc aaatgcttct ttcagtgctg
    14221 attctttaac tggataacca aatttttgga taattgttcc gacaattatc ttaaaaaatt
    14281 gccaattacc atctgcacat tcatattcaa aaatttgcga gaaccaatga ataatctggc
    14341 ttttttctcc tgtatctaat tttttccata ataattgttc atgatgatat aacgctaata
    14401 acattgcggg catttctacc atacgctggt ctctttcatg tatttctccc caataatcgg
    14461 gatgggaagg atctgttcca gcttttattc ctgatagaat taatttcaac catcgttcat
    14521 ctaattggtc aattgcgggc cccgctcccc ataaaacccg agagaatcct tccattcctg
    14581 caattttatt tccatatcct accccttgat gtatataatc aatttgtgcg ccacttgctc
    14641 gataaaacgg ctggagtggt tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg
    14701 tccttaatag tggtactgga tgtttcattg tattttctcc tctttttact accaaatagc
    14761 taattccgac tgttctaatc gaagtaagcc ttcaataaaa aaataatcag aatagatcag
    14821 tggcacatta taagtcagtt ctctatggta tgcttctgct ccgcctccaa tgatcccatc
    14881 ttcatccata ttccaatttg catagtgctt ttctgtcttt tggagtatat tgatagcagc
    14941 ttcatgatac atttttgatc gtacagagga aacatatcct gctaattcca gaagaccgca
    15001 tgcagcgcat actccagcac tggtatcaat attcgaatct tttacagcaa aatcaatgag
    15061 aggaatataa ccagtttttt cgattttctg aataaaattt actgctatct tttctgctat
    15121 agctagatag tacggatttt ttgtgtaacg ataacttaac gcgaatccat ataaagccca
    15181 tgcatttcct ctgctccaac aagaattctc attgtatcct tgcccgccaa gtaaccgaat
    15241 atatttccct gattctgcat caaattctgc gatatgcccg gttgatccat ctttcctcac
    15301 tagatactta gctgttgtat ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc
    15361 agtttccctc gaagcccaat acagtaatgg taaattcatc atactatcaa taatcatcca
    15421 accaggttta tggtcattcc atgctactaa atagttccct gaaatattat agcgtccagc
    15481 aagtaccgta gctgcatgta accctagttg tcgactatgt ttgttttgag actgttgata
    15541 atgtgcaata gccgatggta agaataaaaa acctacatca tgatgaacgt ctgaaaaatg
    15601 gtacagggca gattctaata attgttcttg ttgtattgct gcctctttga acttttctcg
    15661 ttttgtcttg tgaaagagct gccataaaat accactccaa aaaccattgg tccaccagtc
    15721 gattcctctg tcctttatta cgtctgtata ttttccgttg taagaaatat atggaatttt
    15781 attccccatt ctgtctaatt ccgcatctat cttttcagaa gctacattca aaatttttct
    15841 tgaccattcc ttcgtctgtt cattcatgga tatcccgcca ttcttttaat ttttatgaat
    15901 gtggagttgc tcctagaacg aactagatgg ttaacattcg ttattttttt tacttatttg
    15961 cagctccaat gtcattctcc ttaaatttag actgcgccca cacataaatc aagaccattg
    16021 ggatcactga agttgctaaa attagcataa ttgcgccata attaattggc tcacctgtac
    16081 tagaaaccgt catatttttg ataattaagg gaaccgtata tttatcttta tctgaaagca
    16141 tgactaatgg aaatagatat tgattccaac ttcccataaa gataagtaaa ccactagtaa
    16201 taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa aatcacccac tcattggcac
    16261 catcgattct ggcagcctcc atcagtgctg taggaaattg actgctatat tgtcgcatca
    16321 taaacactga agttgtactg gcaatcgaag ggatgatcac acctgcataa gtattcgata
    16381 atcctaaacg actcataatg taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc
    16441 tagatagcat aactgtattg aacaacagtt tattccccat aaaacgatac tttgctaatg
    16501 cataacccgc cattgtagaa ctggctgtag ctaaaactgt actgattaca gcaataacaa
    16561 tcgaattcca aataatcaag ggataattat atctcgccat gatggcttgc cagtttttta
    16621 atagcatatc cccaaacttg aaattgattc cgccacttaa aatttcttga ttactttgcg
    16681 ttgaagctac aatcatatat aaatatggca tcaaaaatat taaggcacca aacacaataa
    16741 tgacgtacgt aaaaattctg ctttgtatct tttgtttagt catttttatc acctccgaat
    16801 ttaaattgaa tagccgataa tataacagat acaaacacca tcgtccaggc aatagcagag
    16861 gcttttccaa aatcaaccat ttcaaaactt gttttatata aattgactcc tacgaccaaa
    16921 gctgaatttt ctggtccatt ggaagtccca aagaatatat ttgggatatt gaaattaccc
    16981 aagccattga tagtggctaa aactgtggcc attaaaatga ttggctttag catggggatc
    17041 gttaataaaa agaattgccg aaatttcgat gctccatcta tttcagcact ttcgtatatc
    17101 tctttagaaa tcccttgaag accagctaaa aataaaatag tgttatatcc tacactaagc
    17161 caaatcatcg tgatgatcat tgatattcgt atccaaaaag gatttgttaa ccactgtaca
    17221 ccgttaattc ctagcgcatt caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc
    17281 atgaatacgg aagctacagc caccgttgat gtaatggttg gaatataata gacagttcta
    17341 aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat
    17401 agcatcgtta ttggaattga tcctgccatg ataatcaaaa cattcgtgta agatttaata
    17461 aacaatggat ctcggaagat agctagataa ttgtcaaatc caacaaattc aaatgagtag
    17521 ccaattttca taaaactaaa agcaaacgag ataatgatgg gaatcaaaat cgtcacaata
    17581 aagaaaataa caaaaggtgt gaccaatcca taagcacttt ttttatgaga cgtatctgtt
    17641 gcttttttct tgctgttctt tattgtcttg cttggtaacg aagattgcat catcgttaag
    17701 tcctcctaat attttttaat caccagttaa ttttacgccg tacttatgag caaaattttc
    17761 tgcttctttt agatatgaat tttcaaaatc acctgtttgc caatagttat atgcagcggt
    17821 agtcaaataa gacgtagcat ctgaataagt ggctggtaaa acagtatttg atgtattttc
    17881 attgatttta ttcaatagtt catagtattt ttgtcctcct tagtagtcaa actttttctc
    17941 agctgcttct gtttgatagg ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt
    18001 ggctaagcat tcttcatctg tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt
    18061 ttttgcatct ttagggacat aaaaacttga tcctcctgat actgcacctt ttcctggatt
    18121 ttcttttgaa aaaggaatca gtggagccat gcgccatttg tcagatgcat caggaaaatt
    18181 caaagacata ttcgtagcat accagccgcc agcaacaaac attgcagatt cttgaaatgc
    18241 tttctcagca tcttgtgcac cgtagaaatg tacaatatct gagtcgatca tttctttgat
    18301 taactttgca gcatgtttcg cttctttagt attcagacgg atatttcctt cttcatcaag
    18361 caaagattga ttctgctggg ctagcatgtt gataaacaat tcttggtccc cagattctgg
    18421 caaggcaatt ccaaaactac cagttttttc ttttaatgct tttgccacct ggatatattc
    18481 ttcccaagaa ctgatttctt caaaatctac acctatttca gcaaattttt ttggttgata
    18541 ataacaaagt gctggggtaa aatcactagc ccaaggaata atcgtttcat tactcgtttg
    18601 ttgacgcaaa atattcaatt tttgatcata aaactgatcg ccatatttgt cataaaatcc
    18661 atattcatca gcactataaa aagcatcttg aaatttttcc agcacacctg ctgctgaagt
    18721 gtctgccatg gagataatat ccggcatatc ttcgtgtgtt accaattttg gggtgatcat
    18781 ttgtactgtt gctgttcccg aagctacatt cacaattttt aagtcaatat ttgctccagt
    18841 tttgcggtta tagatttttg cagcattttc aacatagttt gtcagtttgc cgtttgcgac
    18901 atatacttct aatgtagtgg ctttttttat cattggatct gcgatgtttg ctgttacttc
    18961 ttcgttagtt tttccacatg cacttaagct gaataatagg gtaatgttta gcaaagctat
    19021 caccaatttg tatctttttt tcatgtctat cccttctttc ttttcaagtt cttatataaa
    19081 acgctttcac ttatgtatat
//
LOCUS       DAZBWR010000053        18572 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000053 DAZBWR010000000
VERSION     DAZBWR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.053"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(181..1947)
                     /locus_tag="ACHGT5_001588"
     CDS             complement(181..1947)
                     /locus_tag="ACHGT5_001588"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217576.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1907..3661)
                     /locus_tag="ACHGT5_001589"
     CDS             complement(1907..3661)
                     /locus_tag="ACHGT5_001589"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217577.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3771..4340)
                     /locus_tag="ACHGT5_001590"
     CDS             complement(3771..4340)
                     /locus_tag="ACHGT5_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN7217578.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4358..5773)
                     /locus_tag="ACHGT5_001591"
     CDS             complement(4358..5773)
                     /locus_tag="ACHGT5_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217579.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5784..6452)
                     /locus_tag="ACHGT5_001592"
     CDS             complement(5784..6452)
                     /locus_tag="ACHGT5_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFN7217580.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6583..7167)
                     /locus_tag="ACHGT5_001593"
     CDS             complement(6583..7167)
                     /locus_tag="ACHGT5_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7217581.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7502..7732)
                     /locus_tag="ACHGT5_001594"
     CDS             complement(7502..7732)
                     /locus_tag="ACHGT5_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN7217582.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(7747..8697)
                     /locus_tag="ACHGT5_001595"
     CDS             complement(7747..8697)
                     /locus_tag="ACHGT5_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN7217583.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8694..9536)
                     /locus_tag="ACHGT5_001596"
     CDS             complement(8694..9536)
                     /locus_tag="ACHGT5_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN7217584.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(9669..10394)
                     /locus_tag="ACHGT5_001597"
     CDS             complement(9669..10394)
                     /locus_tag="ACHGT5_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN7217585.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(10855..11169)
                     /locus_tag="ACHGT5_001598"
     CDS             complement(10855..11169)
                     /locus_tag="ACHGT5_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN7217586.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(11185..12780)
                     /locus_tag="ACHGT5_001599"
     CDS             complement(11185..12780)
                     /locus_tag="ACHGT5_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFN7217587.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(12803..13693)
                     /gene="iolE"
                     /locus_tag="ACHGT5_001600"
     CDS             complement(12803..13693)
                     /gene="iolE"
                     /locus_tag="ACHGT5_001600"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFN7217588.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(13710..14735)
                     /gene="iolG"
                     /locus_tag="ACHGT5_001601"
     CDS             complement(13710..14735)
                     /gene="iolG"
                     /locus_tag="ACHGT5_001601"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFN7217589.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(14759..15766)
                     /locus_tag="ACHGT5_001602"
     CDS             complement(14759..15766)
                     /locus_tag="ACHGT5_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN7217590.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(15879..17021)
                     /locus_tag="ACHGT5_001603"
     CDS             complement(15879..17021)
                     /locus_tag="ACHGT5_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFN7217591.1"
                     /translation="MPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTGHLQKIK
                     ELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTAKVVAVL
                     AANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKMMMKDLV
                     FMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEAMKKILN
                     TLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPHGISNAM
                     LLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLSIPTIRD
                     YGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(17115..18569)
                     /locus_tag="ACHGT5_001604"
     CDS             complement(17115..18569)
                     /locus_tag="ACHGT5_001604"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFN7217592.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         5734 a         3860 c         2886 g         6092 t
ORIGIN      
        1 ccaatttttt aaaactttga ttattcctta tattataaga atagacatta agtgaactaa
       61 ttcaatcata ctgattcttt cgtgtattca aaattagatg ctgtttaata aaattattct
      121 ttttgaaaaa gaattataac tatatcccaa aaattgtagg ttaatttaaa gcctatttca
      181 tcatcgtgat tgagcttctt tttcatagac gaatttctca gatagtttag ttttctttat
      241 gagatttttg tatatttctg accttcgata caactcacta tgagtacctt gatcaactat
      301 ttttccttga tccatcacta caattttatt taccttttca atagacttca agcgatgcga
      361 tataattagt actgtcttat cttgtattaa acgatttaaa ctttcctgaa tacgtttttc
      421 attttccgca tctaaactgg cagtaatttc atctaaaatt aaaattggcg catttttcaa
      481 aaaagcccgt gctatggata ggcgttgtcg ctctcctcca gaaagtgaac ttccgttttc
      541 tccgaccaat gtatgataac cgtcaggtag tttttcaata aagtcttcgc agttagctaa
      601 acgagctgct tttttcactt cctcatctga cgcttgacta tttccaatac gtatattttc
      661 caaaatactt gtattaaaca aagttacctc ttgaaaaaca atagcgattt tgctaaacaa
      721 agaagctgga gatacacgtt taatatcata cccatcaatt aaaatacagc cttcatcata
      781 atcatacagt ctagacacta atttcaagat actagttttc ccggaaccac tagcacctac
      841 taacgcagtc acttctcctt gtttagccgt aaaagagata tgatctaaaa ttggggtatt
      901 gttatcatat gagaaagaga catctcgcaa ctcaatgtca aaagatttca gaggactatc
      961 tgatccctct tggatactgg tttccttcat agctctaatt cgctgtattt taggagctaa
     1021 ataaaatatt tcaagcactg cttctttcat agaatcgaag gaatccttta tttttattgc
     1081 ggctagtaag taaccaacga cgtagagtat agtcacttca cccgttaaca gtaagtgaac
     1141 acctactaga atgactactg ctaaactaac atatgagaaa atagaggata aggccatgac
     1201 tgaaaaagta cttaattcta cctttaaatg aatcctttca ctctcttcca tttttttgaa
     1261 aagtctgtct tgaacttttt tagatagatt aaagctatta atctcttgct gcaattctat
     1321 agtttcttga aattcttccg agttttctct caaagtatcg taatatttag tattggcttt
     1381 cgtttgggat ttctttgata acaagattaa gacaaaacta aataacgttg gcaaaataac
     1441 agctaatccc attttgacat taccaaccaa aagcatcact gaaataaaag ggaaaaacag
     1501 agccataccg ccggctttag gtattgcatg actcatcgca tgctcaatac cttcaacgtc
     1561 agacatgata gtttgagata aatctgataa attatgtttt gaaaaatagg ataatggtaa
     1621 atttgacaaa tcgtctgcaa tttgcattct taaatgcgca ctttctttat aagtactatt
     1681 atataagttt tcatactccc tatctaacaa ccaatacatg aaaagcaaag taacgaacga
     1741 tactgtaaaa taaagccaag gcggctttac attttctaaa actaattcgt ctatcagcat
     1801 cattataatg aatgctggta ccatattaat gcagtataca aaaaagctag aaatagatgc
     1861 tttaaccaaa tcttctgcgc cctgattagt taaagcgaat ttgtttttat aaaaactttt
     1921 cattgtcaag cctccaatca tttgcattct cataattagt gaccaattgt ttatacactt
     1981 gatgtttagc cattaattca ttatgatttc cacgttctac tattttccct tctgacaaga
     2041 caagaatttc atctacagat ttaatgctgg ataatcgatg agctatcata ataactgttt
     2101 tttctttcat cagatgttga aaagcctttt gtagttcata ctcattatct ggatcaatag
     2161 atgcactggc ttcatccata ataacaatct ttgcatcttt taaaattgct ctggcaatag
     2221 ctatacgttg tttttctcct cctgacagat aaactccttt actaccaatt aaggtatgct
     2281 ctctttcggg aaatttttct aaaatcgatt cgcaaccggc taatcttagc gcgtcaaata
     2341 ctttacttgc tggcgcttga ggatttgcta tagaaacgtt atcgtaaata ctctgattga
     2401 atagtttcgt atcttgaaag acaaaagcaa ttgtgttaat aattgtgtct tttgaatagt
     2461 gatttaaagg cttaccacca attttaatct gtcccttatc cacattgtag aaacctgaaa
     2521 ttagtttagc tatcgtactc tttccgcttc ctgattttcc tactaatgca taaattcttt
     2581 tttgttctaa aactaagttg aaattttcta aaacacactc ttctttgtat gaaaagctga
     2641 cattttcaaa tgcaatgtca caatttttaa tctgtgtctc gttcccatat tttagttggt
     2701 cttgcttcat ttctaaataa agggagtcta gtgtttcgac tgcatatctt gcttcaaaca
     2761 agtgcatact tacatacatt attctcatga aagaaacaaa aataattcca cttaaaaaaa
     2821 acaacatcaa caagtcaaca gctaatatct tgggagtggt aagagaagtt ataaaaaagg
     2881 ttataggaac tatcaaaatg caaactatgc caaaaaatag ccattgaaaa agaacaaaag
     2941 gtgttttccc actcaaagaa tacttatatg caaacttagc ataatcgtta attgctttat
     3001 tcaaggcttt aaatgatgtt actttagcac caaatatctt tataacttga attcctcgga
     3061 tatattctac cgtttcagag cttaacacat ctaacgattc ttgatatttt tgaataaaat
     3121 ttgtattctc tgtcattttc tttaacaaga atacaccaat tactaacatg gcgattagaa
     3181 ccaatccaat tttaaaatta atgaaaaagc ctaaggcaac tgataaaaca ggtgttacta
     3241 tggcttgagc actatctgga atcaagtgag caattgccat atgtgtttta gccgcgttgt
     3301 catcaattgt ctttcgaata tatcccgaag aatttaaatc aaaaaaacga aaacttgctt
     3361 ctgttaaacc gctaattccc ttttttctca aattagtctc taagcgaaac ccaaggatat
     3421 gagaaaataa gccagaaaaa atataaaata aagctcctaa agttaaccaa gcaacagttt
     3481 gaaccgcata actcttagct aaatattcat ctcctacaat aattagagac ttaagaaatt
     3541 tgtaaatact gtaatatcca tagactgtta atacgacaga aaaatcagaa aataaagtgg
     3601 ctaaccatcc gtagtatttc attttaggga cataactaaa taattttttg tatactttca
     3661 ttttttcctc ccccgcattc aatttcttct cttattcgtt tacaactgtt aatgctttcc
     3721 gtaatagcgt aaattagaga tgtacaaaca gatagaaggt aaactacgtt ctattttttg
     3781 tattttttta agacttttct tccaatcaat acaccaatca tagataaact tgctacaact
     3841 aacactgaaa tgattcccca tatacctacc gcatagtgga tcattgcatc cattgttgca
     3901 gcagattgtc ctttatctat ccaatcttga cgaaaacgat ctgtaaagaa ccatatagga
     3961 atccactgtc cggcagcact gcctaattgc attagagccc aagacgcaac ttgaggaatt
     4021 gctcgatcat atccaaatac agatagaata atatcagcta agatcgccat actaatcgaa
     4081 ctgataaacc aaggtaagta cgccccgccc attaacgtaa aaaataacaa taaaacaatt
     4141 gtgtaaatag taaaaattcc ttttctaggg tagttatagc taatataaac aaatatgatt
     4201 ccagataata atccaaaaat ccctggggat atgctgtgac caaacgcccc accaatacta
     4261 ctaaccatta ttgaaaaaaa gtaaattact agggaaataa ctgtagctaa accaataaaa
     4321 atataatctt ttgttttcat atatattctc ctcaattcta tttcgttatg tcattaaaaa
     4381 tctgttctat caaagttgaa tcttctactc taagttttat atcttttttt attccaccat
     4441 tttttaaata aataattcta ttgcatgttt tataaagaaa ttccggatca tgagtggcta
     4501 taactactgc acccttttct tttaagtctt taataaggtc agcaatcctt tccatattaa
     4561 tataatccaa cccacttgta ggttcatcaa atagaaataa attagttttt gacaatgaag
     4621 ctatagaaac caataatctt tgtttttctc caccggataa atcaaacggt aattgatctt
     4681 tcattttata aagttccatt ttttttaaaa ttttttcttg ctgatcttta ctaactattt
     4741 cattccccag ggctaactcg ctcctcactg attctgtgaa aaattggtaa tccatttctt
     4801 gcattactaa aaacggcgat gacaaatttt ttttattttc gattatcttt cctttggtag
     4861 ggcgaataat cttcatgagt attcttagta aggttgtttt ccccacaccg tttttcccta
     4921 ccaaaccaat aacatctcca actttgacat caaaatttat gtcttttaga atattttcat
     4981 aagtaatttc ttcaactttt aatacatctg cacttacctt ttcgatatag ggaacttcaa
     5041 tcgaactatt aaaaatgtca aatttcctta atgcgttatt gttttcagtt tttaacaact
     5101 gaatagttgt agtttttaca gttctatttt taacaaatag aacttgatca attaattctt
     5161 tcaaatagta aaaacgatgt tcagctataa caatagtaaa tcctttattt tttaaatcac
     5221 aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa atttgaagac ggttcatcca
     5281 atataattaa tttagagttt agcgataaac tacatccaat agaaattttc tgccgttcac
     5341 cacttgatag cgaaaaaatg tttttatcta atatatttga aagatctaat aattcagcta
     5401 attcattgat tcttttatcc atatgctctt ttgtaaatcc aaagttttcc atcgaaaata
     5461 ctaactcact cgttgtattt gttgtaaaga attgtcctct aggatcttga cttacgtagc
     5521 caatattttt tgatatactt tcttgtgtta aggattgtag cgagttattt tcaaataaaa
     5581 tttccccaac caattttccg tcataaagtc ttggaataag tccattcatt aactttaata
     5641 gtgtggattt cccactccca gaagaaccag taatcaatgt aagactacct ttttttattg
     5701 aaaaattaag gtcaacaagc gcttgttcct taacatcctt atatgaaaaa gaaacattgt
     5761 taaattcaaa caattctttc acctcaaatc aaaatactta agaaaaaaat agtaacggaa
     5821 aacaggatga tatagtctaa taagctcatg tttgttttat agtaagaact tcttttcaca
     5881 ggagattcta tcccctttgt tattgcatta gcggtcattt cttcagcaat catagttgca
     5941 cgtataagaa cgggaactaa cagaaattcc aaatattgca gaggctgaaa aaaagacaag
     6001 cgaatgtttc tcatttttaa agattcttta atcattctta tttcagaacg ataagtcgga
     6061 aagaatctga tcactactgt aatacttaac attatttttt tcggtaaatg tattttttct
     6121 aaagaggata ataattcatt gctagtatag acactaacca acaaccatga gatcattacg
     6181 accggcaaca ttcgaaatac aatatataag gatgtcccaa aaaatatagt aaaaaactct
     6241 atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat caagatgaaa
     6301 taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat aatgaaacac
     6361 cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa
     6421 gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa taagatcgca
     6481 ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga tcttatcaaa
     6541 aagcataact gagaacaatt ctcaatgcaa gctttttttt aattaactca gtaattcaat
     6601 ccacccagga acagaaaatt gatacaatct tctggttaat agcatagtct tttcttttga
     6661 atagctatgc ttcaatatat catagaatgt agcgtagaat gagtaaatgt agaagtggat
     6721 ttcttcttca ttaaagacta aattttgatt gatcattccg atagtcttta acttatctag
     6781 acaacagact gtcttttttg tcacaaatga tgtaattttt tcaacaatat cttgatattt
     6841 ggttccagct gattggaata aaaccaaatc gaatatacgt ttattctcaa aaatataatt
     6901 aataaatgga tctaattcat tgaagttctc tctccaactt ttctctatat ctatattttc
     6961 taagtttaag aggtatttgg taaactcttc attatacata ttaataagcg cattaatatg
     7021 cggctgaata atttcttcaa ataacgcttc cttagaagta aaatgtttat aaaaagcacc
     7081 cgtagttacg cctgcggtca agcacagttg ccttatctta gtttgctgaa atcctacttt
     7141 ttcaaaatgg cttagtccac tttctattaa tttttggtga gttgtaatat aatctctggt
     7201 ttctatgggt acttcctcct aataagatga attaactata ttatcttaca caggacttga
     7261 agtttcaatc gttaacgtct attactgaac tcattcctta gagctaaact aatgagaaag
     7321 agcaaaataa ctgatacaaa aagcaatcct acaagaatta atagactcat ctaaatcatt
     7381 caccttttct aaaataatcc gagaaagatt taaaaaaatt atatccagct attaggataa
     7441 gtcctatcat aatcactcca atagccattt tcttatcctc cctaaaaata aagcctttta
     7501 cttaaaacct ttttttgtaa tcgtcaataa attttttccc tttatcagtt aattgaaatc
     7561 ctatatctaa atagtcctca tatctaagtt tagtcagcat ctcacttaca gatgctggac
     7621 taacacttag aacttttgct aaatcattat tttttaattt cttttttctt tttgataatt
     7681 cctgaattgc tagtaaataa attagtatag tagttgtttt aatttgtacc atacctaccc
     7741 tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca tcatcgcata
     7801 gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg ttgaatagat
     7861 aggaatattg gtatcttttg atactgtttt cattggtcta tcgtccacac tgctttctac
     7921 gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg
     7981 tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat tatacgcttt
     8041 cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat tcggaattga
     8101 agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat acttatctag
     8161 attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt tttcaatatt
     8221 tttagcgtaa ataataccgt tttctaaatt taaccaagca tggggatctt ccttcccttt
     8281 ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa aatagtcttt
     8341 gttttcctct ttgttcgcat tttttactaa ttttgtaaac caagcatttc ctccagtttc
     8401 caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg
     8461 caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt
     8521 atcttttgct atattttcag taatatctgc taaaatcgaa ttagtaacta ctacctttaa
     8581 tttttcgttc gaagccactg tatctttgtc tttactattt gttgaacatg ccccgaatat
     8641 aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac
     8701 tttctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat
     8761 aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaatg
     8821 aacaaaccta gcaatgatga gcacgctccg actgtcgccg aaataaatat catagttttt
     8881 aaactgtttg catataaata tgctgtagca gctggtgtaa ttaacattgc aacaattaat
     8941 atagttccca cactttgcat agctgttact gctactaaag ttaatagaat cattaataga
     9001 taatgataaa aactgacatt catccccatt gcttttgcca ttaatggatc aaaagaagtg
     9061 attaataatt ctttaaaaaa tagaacaact gtaaccaaca ctaaaacaga aacgccaatt
     9121 gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataagat atgaaataaa
     9181 tccgttgagc tatttgcaac tcctataagg ataactccta atgctaaaaa agaactgaaa
     9241 gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg
     9301 atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa
     9361 gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct
     9421 cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac agatgtaatt
     9481 agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg aagcatatta
     9541 ttccccttgt atattagagt tagggtttta accttccggt tttgattgga taaccctatg
     9601 aaccatcgtt attttaattt ggtattaact catttctttt gagataatat atttattgga
     9661 ttgagaggct aagccgttcc cccctctcca ataaatatag tatctccata ggcttttttt
     9721 aaattctctt cattaaatac agattctaca cttccgtgag caattagatt tcgattcaaa
     9781 agaataatat tatcaaaata cttctttacc ttgcttaaat catgatgcac aattagcact
     9841 gtttttccct gatgtttcaa ttcttttaac gtatccataa ttattcgttc actaactaag
     9901 tctatcccta caaatggttc atctaaaaat atgaaatccg cattttgagc aagacatctt
     9961 gctaaaagta cacgttgaaa ttgtcctcca gataattctc ctatttgatg attagaatat
    10021 tttcccatat cgaccttttc caaagccttt gaaactcttt gccattctgc atttttaatt
    10081 ctttgaaaaa ctttcattcc ggcatatgtt cccatagata cacattcttt tacttttatt
    10141 ggaaaagtaa aatcaatatc agctttttgc tctacatatg caatcttcac cagtttttta
    10201 ttgatatctt cattattaat ggagacctct ccctgatgag ggataatatt taacattgct
    10261 ttaagcaatg tagattttcc agcaccattt ggaccaataa tacctgttaa agcaccttct
    10321 tgtattttta aagaaatctc gttcaatgct aaaaaatcgt tataacaaac attaagcttt
    10381 ttcacttcta acataacttt ctccttctaa atgataatga ttatcacgat catatggtat
    10441 acaaaaatta tattttagtc aaccctaaat tttataattt agattttttt ctgttgttga
    10501 agattaggat gtcaaaatta tttgaaacta ctcgtatagt aaatagtaag taattttcaa
    10561 aacacgaaga aaaacaaaaa gaaaaaagga aacgccgaag catttccttt cagtgattta
    10621 atgagaacaa cacgatttga cttagagtct caaaaagtcg aataggtaca ctcatttaag
    10681 tgtctccatt cgactttttg gggatgagct ggctattgag gtccgcttac cctattgaat
    10741 aaatacaaga ttgggtattt ctaataagtc cgcccttcat attggggtga tcggggcgaa
    10801 catgccatct tttcatatac cacctcctcg acactcaata atcaaagatt ctcgttaatc
    10861 ttcttcgatg cttgcaatga tgttttcaat attcatataa cgaaaatctt cttgtttgat
    10921 ataaacgatc tgagcggtac atttttcctt tttgatgatt tcttttactt tttcctcaga
    10981 acgtgcgacc tgcggcgtta aaagtaaaac gtcatagtct ttataattca gtttttcttc
    11041 aacgtttcgg atcgttgcat aatctatctc atacgataga tttttggctc tgatcgtttc
    11101 aagcattttt gatacgagca gcgacgcact gattccggtc tcgcaaataa tcaatagatc
    11161 cttatgcata ataccctcct gtttttagcc tgcaaagcca attggtgaaa atgccacata
    11221 agttaaaata acaatgacta ccaatgctat agcagtcacc ttggtgtatt tccaaggctc
    11281 gatatcgacc tttgtatcga atgagttcaa cacaaattct ccatcaggtt tcaatttgga
    11341 aaccaccatc aaaatgataa tgtccagaac gaataatgcc gtcaaaacat aaaggaaatg
    11401 gatattttgg aaaagcattt ttgatagtgc atacaagacg atatgcacgg caatcgtcgt
    11461 ttttgcccca aaagcagttg ctttttttga ataaaaggca aagagaatga tcacgagcag
    11521 cggcatgttg tatagtccat tgaattcttg aacgaactga tacaatccag ccggtgcaaa
    11581 agaaatgaat ggcgctacga taatggagat aatccccaca acgaccgtta ctgttttacc
    11641 agcacgagcg atttgcttat catttgcttt cttattgata agcggtttat aaaaatccag
    11701 tgaaaacaag gtcgatgtcg cgttcaaagc tcccgcaaat gatgataaga tcgcgccaaa
    11761 gataacagcg gcgaaaatac caaagatcat tttaggcaat acttcactga ctaaaaaagg
    11821 atacgcgtta tctggatttg acattaaact atcgccaaaa atattgcgag cgacgatccc
    11881 tggaaagaca aggaataacg cacctaagat tttgaagacg ccgactagga aagcgccctt
    11941 ttgtgcctca gctaaattct tgccggacaa tgctttttgg acgatcattt gattggtaca
    12001 ccagaaatac agattgttaa acagcatccc tgtaaatagt gtcggccatg gaacaacagc
    12061 cgagtcgatc gatcccacag agttaagcag ccatggcgtg tttaccacaa cgtgttcgat
    12121 cccgccaaca aagctgccgt caccaagctt cattaagcct aaaattggga ccagcagacc
    12181 acaagccagc agaccgactc cataaatgga gtcactattg gcacttaacg acaagccgcc
    12241 gatcagcaga tagaggatgc cgacaatacc aattgccatc gctacaatta tgatcgcgac
    12301 gatcggctgg acgcccagca gctcatcaat atggaagatc ttattgaaca ccagcgatcc
    12361 agaataaaga accacgggta agaaagaagt catataggtc acaataaata agatcgaaat
    12421 aattcgtttg gtaaccgtat cataacgaat ctcgatgaaa tctgagattg tattgatgcc
    12481 gtatttaaaa tattttggca agaatatcac agccaaagct acgatcgcaa tcgtagcagt
    12541 tacttcccag gccattactt ccatccctgc atgataactt tgaccatttt gtccgactat
    12601 ttgttcggtg gacaggttcg tcatgatgat cgtgccggcg attggcaaag cagttaagct
    12661 ccgccctccc atgaagaacc cttctgatcc agaagtatcg acacttctgg atctagaata
    12721 ggcaaataac catacacaga tgacaatcac taggaaactg attatagaaa aaagattcat
    12781 cgtctagctc cttttcaata gattaaagac cggtgacctc tcgaatgtag cttctggctt
    12841 tgactgcata ttcaaatgga ttggctttag ccgggtcctg ttctgcttca actacgatcc
    12901 agccttcata gtcgctggca tggattgctg tccagattgg agtgaaatca atcatcccat
    12961 caccaggaac cgtgaagacg cctttcttca cgccgtttaa gaagctgtct ttttcctttt
    13021 ctacttcttc agccgtttct tgacgaatat ctttgaaatg gatgtgtttg atacgatctt
    13081 gatgacggtt gtaaaccgcg attggatttt caccagaaaa gaccaaatgt cccgtatcga
    13141 acaataattt aactttgtct ggatcagtca tttccattaa acgatcgatt tcttccgtcg
    13201 tttgaacgcc ggtacccatg tgatgatggt aaacgatctc catatccttt tcgtgggcca
    13261 aatcccctaa tttttctaat ccttccgcca gttttttcca ctcttcatca gtgaaaactg
    13321 gtttatcatc aaagactggc gctgtcataa tcccttggat actatgaccc tgctcagcta
    13381 ctacgatgac tttagcgccc atcgcatgta agaaatcccg atgtgctttg aaggcttcgg
    13441 cagtttcttc aaaaggttta gttgtcagaa acgcactgaa ccatgcactg gcaaccttta
    13501 agccgcgaat attcaaatat tgttccaaca cctctggatc ttttggatat ttgttgccaa
    13561 tctctgttcc ggtgaaccca gcaagggcca tttcactgat acattgttca aaggtatttt
    13621 cactaccaag ctcgggcata tcatcattgg tccacgcgat tggtgcgatg gctaattgaa
    13681 tttgtttggt catattctca cactcctgct tattttttta gttgtactaa actcttcgta
    13741 tcgaatgatt cttgacaagc caacgcgatc ttagttgatt gcaatccatc ttctgcatta
    13801 accgctggtt gacgtttttc ttgaatacat gagacaaatt cttgggcttc acgaatgaag
    13861 gcttctctga atcgttctgg gaagttttgt gaacatggac ggatgatccc ttgatcgttc
    13921 atgatcgtca caagattttt ctcaggcgct tgcgctaccc gcagcattcc tttggttccc
    13981 atgatctctg tttcgacatg atagccatgg gctgcgtttc tgccggcaac tagtaaagct
    14041 attgttttat cgctcaggct catcattgcg gctccagttt caagctcacc agcctgatcc
    14101 aattctggat acgccgcatt tttcccaatc gcccacactt gatcgacttc ttggccggta
    14161 aaccagcgaa ccaaatcaat atcatggatc gccatatcta agaacaagcc gccgctgtcg
    14221 ttattcatgg caaatttgac aaagctttcc aagccttcgc tcggatcgat cccataacaa
    14281 cgaatcagtg tgatttcgcc gatttcgcca tcttctacca ttttcttcgc atactgatac
    14341 gactcatcat agcggcgcat gaagcctaac atgaagacct gttcaggatg ctcttcgatg
    14401 actttttggg tctttttaat atcttcaact tcaaggccta atggtttttc agtaaagaca
    14461 tgcttgccat tttccatcgc caagcggact tggtcacagt gaaagcctga tggtgaaaca
    14521 ataaagactg catcgatcac ggaacttttg accatttctt catagctgga aaaagtatgc
    14581 ttgacgccta atttcttctg tgcgaatgct aattctgttt caactaaact gcacgcagcc
    14641 gtaagctcac aacaactgac acctgttgct aaattttgtg catgtacttt tcctaatctt
    14701 cctaaaccga cgatgcctac tctaatgttg ttcatgaaat acctccgttt tctgagaatt
    14761 actcattgta tagtgctggt cgttcatcca tttgaatcat tacttttgtt tttgtttcac
    14821 gagactcatg acaagccatc atggtgatcg ctgctacata cccatcccaa gcggtaggac
    14881 cgacaaccgc atcatttttg atcgaatcga tccacagctt gaattcataa tcataggcat
    14941 ccaaaaagcg ggtctgccaa tctggtgaaa caggtgtgta atttttttct tccgatttaa
    15001 ttcgtgtata ggctgggtcc gtcattccta cttctgccgt ttcgaaaacc acatcacatt
    15061 taatatcgta gccaaagcgg caattcacaa agatttccaa atcaatcgtt acaccggatt
    15121 ttgtttgaaa gatcagcagc tgcggatcat gcaaatcggc atgggagttg ctggtttttt
    15181 tcggcaagat catttgaaca gattcataat cttcatttag caaccagcga agcacatcaa
    15241 tctcatgaat cgcggtgttg ctgatcgcca tcggtgttgt gtagttttca tctgcgctag
    15301 gatttctatg ggcacaatgc atcattaatg cttctccgta ttgcttcgat tcgattgctt
    15361 ctttcagttc aatgtagccg cggtcatagc ggcgcatgaa gccaacctga accaatttct
    15421 tcccataagc aacttccgca tcaacgatcc ggcggcagcc tgctgcatcc atcgctaatg
    15481 gcttttcgca gaaaacatat ttcccatgct tgattgcttc taacacatac tgctcatggg
    15541 tcgggtccca agaagtcaca acaacgacat ccacatcttc agaagcgatc aatgcttctc
    15601 cggtttcaaa gaatttagca tcgacacgtt tggcaacctc tttagcagct tttacattga
    15661 tatctgagac tgctgtcaca taagcccctt gtgttttctg attgatcctg tcgatatgct
    15721 cgatgccgat tgcacctgtt ccgataacgc ctacttttac tctcaattac ttatccccct
    15781 gttctgttgt ttaagcacac atagtatgcg cttacaaaat tattcctgtg tagcttttat
    15841 tcttaaaagg tagagccctc tctttgctcc gccttttact acccataagc ttgtacgtat
    15901 aattccttaa tttgactatg actaggcacc tttttattat tttgcggact tccgctggtc
    15961 aacgcatccg cggccatttt gtctaaggct gccataaacg cttgctgatc aataccataa
    16021 tctcgaatcg ttgggatcga cagctgctca cagagctctt ggattttttt aatcagataa
    16081 tccgcagcct cttccagcga taaatcttcg gctgccggat aaataaatct ggcgatcgtc
    16141 gctaactcag tctgaatagc atcctttgag aattcaaaaa agaccggcaa taacatcgca
    16201 tttgatatcc catgaggaat gtgaaaaagt gcccccaccg gtcgactcat tccatgaatc
    16261 aatgtgactg acgcattcga aaaggcaatc cccgcctccg ttgaagctaa gctcatttgt
    16321 tctctggctt cagtgtcgtc gctattttca tatgcctgag aaagcgtgtt caaaattttc
    16381 ttcatcgctt ccagtgaaaa tagttgcgtt acttcattgg ctttgactga aacataagat
    16441 tccaaagcgt gacatagcgc atcgactccc gttgccgcgg ttacttttgg tggtacagtt
    16501 ttcgtaaaat cagagtcaac gattgagatc tgtggcatga aaacgagatc cttcatcatc
    16561 attttgactg ctgtttttgt attggtaata actgccacat cggttacttc cgaaccagtt
    16621 ccagcagttg ttgacactgc aatcagcgga atgcccgctt gcagctgatc aaaatttctt
    16681 gcatgaatcg cttcgagact taattgatta gcagccaaaa cggctaccac cttggctgta
    16741 tccagacagc tgccgccacc aaaagcaatg atatgatcac attgatgctt ctgaaaaagc
    16801 ttcagcgcat cataaacatt ttcttgcgtc ggttcggctt gaatgtctgt atactccgct
    16861 aattgatcat ctgtttttcc caacagctcc ttgatctttt gaagatgccc ggtctgttcc
    16921 ataaccgtat cactaatgat taacggcttg ttgcctagtt gttctgattc ttccataaga
    16981 aattggattg ttccgccgcc aattcttaaa ttggcaggca ttttcagttg aatattcaca
    17041 gaaaaaacct ctctctggct tatagcctat aacagaatcc tcgaagctct ttaacagagc
    17101 ttcgaagcaa atctttaaat ctcactttgc gcataacgtg ctgtaacaac tttccgatgg
    17161 gtataaaact caacactgtc tttgccattg gcatgcaaat caccaaagaa agagtgcttc
    17221 cagcctgaaa aggcaaacca agccatcggt gcaggcaccc caagattgac ccccagcatc
    17281 ccagcatcaa cattttctct gaagtagcga atcgctgatg cgttgttggt atacagacac
    17341 gaaccattcg caaattcaga ttcattcgtc agctcaacgg ctgccatcaa atcttttacc
    17401 cgcacgattg atgcgaccgg ggcaaaaatc tcatcctgcc agatcttcat atccatcgtg
    17461 acattatcaa aaatcgttgg tccaacaaaa tagccttttt ctggaatgtt ttcgcgacca
    17521 tctagtacca gtttcgctcc ttcatcgacc cctttttgga tatagtcaaa ggtccgctgc
    17581 tgtgcatctt ttcgaatcac tggtcctaaa aagacccctt cgtccaagcc attgccgatc
    17641 ttgatcgctt ttgccgcagc cttaaatttt tcaacaaact gatccgcgat ttcctcttcc
    17701 aaaacaatga cggaggctgc catgcagcgt tcaccagcag aaccaaaagc tccgccaata
    17761 aggtccttga ctgcccgatc aatattggca tctttcagca caacaacatg atttttcgct
    17821 ccagtcaacg cttgtacccg ttttagattt tttgttcctt cacggtaaac gtattcaccg
    17881 acattttttg atcctacaaa gctgatgcct tttaccagtg gatcttgcag caatgtgttg
    17941 acaacatcgt gggcaccata gacaacatta aaaacaccgc taggaaagcc tgctttttca
    18001 accagctcaa caattttctc catcaacaat ggtgtttttt ctgaaggctt cagcaccacc
    18061 gtatttcctg ccgccagagc catcgggaag atccagaagg gcaccatcat tggaaaatta
    18121 aacggtgtga tccctccgat tactccgatt gggtaacgat aattcgtgat ctcaatatca
    18181 gtcgccgctg ttgataaaga atctcccatc attaaattgg tgattcccgc tgcgtgctcc
    18241 acattttcga ttcctcgttg aacttctccc agtgactcgg acaatgattt cccattttcc
    18301 agcgtgatca aacgcgccaa ttcactttta tgatctgtta aaagctgatg cagcttgaac
    18361 agataacgtc cgcgaacagg aacaggaact tttttccaag tttgaaacgc agcatcggca
    18421 gctttaactg cttcggctgt ctctgctttg ctagacaacg gtacattcgc cagcgcttct
    18481 tctgtagccg gattcattac ttcttcaaac tctgttgagc gactagcgac ccaacgacca
    18541 tcaatataat ttcttaaaaa tcttgtcatt ta
//
LOCUS       DAZBWR010000054        18294 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000054 DAZBWR010000000
VERSION     DAZBWR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.054"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1650
                     /locus_tag="ACHGT5_001605"
     CDS             <1..1650
                     /locus_tag="ACHGT5_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7217593.1"
                     /translation="FTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFNLVVP
                     QEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTYKIKL
                     REDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKSELGI
                     KAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNGPFTL
                     EDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDVILSG
                     ELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVKQVLG
                     DGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDSLEFD
                     LMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGGWAAD
                     YADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIKIISD
                     DMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1720..2067)
                     /locus_tag="ACHGT5_001606"
     CDS             complement(1720..2067)
                     /locus_tag="ACHGT5_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN7217594.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2151..3092
                     /gene="opp3b"
                     /locus_tag="ACHGT5_001607"
     CDS             2151..3092
                     /gene="opp3b"
                     /locus_tag="ACHGT5_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN7217595.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3093..4136
                     /locus_tag="ACHGT5_001608"
     CDS             3093..4136
                     /locus_tag="ACHGT5_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217596.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4142..5212
                     /locus_tag="ACHGT5_001609"
     CDS             4142..5212
                     /locus_tag="ACHGT5_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217597.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5202..6155
                     /locus_tag="ACHGT5_001610"
     CDS             5202..6155
                     /locus_tag="ACHGT5_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217598.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6167..7177
                     /locus_tag="ACHGT5_001611"
     CDS             6167..7177
                     /locus_tag="ACHGT5_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN7217599.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7462..8283)
                     /locus_tag="ACHGT5_001612"
     CDS             complement(7462..8283)
                     /locus_tag="ACHGT5_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN7217600.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8380..8658)
                     /locus_tag="ACHGT5_001613"
     CDS             complement(8380..8658)
                     /locus_tag="ACHGT5_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217601.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8926..10935
                     /gene="metG"
                     /locus_tag="ACHGT5_001614"
     CDS             8926..10935
                     /gene="metG"
                     /locus_tag="ACHGT5_001614"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN7217602.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11414..12001)
                     /locus_tag="ACHGT5_001615"
     CDS             complement(11414..12001)
                     /locus_tag="ACHGT5_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217603.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12176..13498
                     /locus_tag="ACHGT5_001616"
     CDS             12176..13498
                     /locus_tag="ACHGT5_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN7217604.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13653..14429
                     /locus_tag="ACHGT5_001617"
     CDS             13653..14429
                     /locus_tag="ACHGT5_001617"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN7217605.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14422..14991
                     /gene="rnmV"
                     /locus_tag="ACHGT5_001618"
     CDS             14422..14991
                     /gene="rnmV"
                     /locus_tag="ACHGT5_001618"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN7217606.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            14984..15868
                     /gene="rsmA"
                     /locus_tag="ACHGT5_001619"
     CDS             14984..15868
                     /gene="rsmA"
                     /locus_tag="ACHGT5_001619"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN7217607.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16319..16948
                     /locus_tag="ACHGT5_001620"
     CDS             16319..16948
                     /locus_tag="ACHGT5_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217608.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17072..18181)
                     /locus_tag="ACHGT5_001621"
     CDS             complement(17072..18181)
                     /locus_tag="ACHGT5_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217609.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5955 a         3307 c         3742 g         5290 t
ORIGIN      
        1 tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta tggcggcagt
       61 tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg cgtatttaac
      121 ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga tacaatcagt
      181 tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga cagcaaacca
      241 cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac ttacaaaatc
      301 aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc ggattacgtt
      361 tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta cctgtatgcg
      421 ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga actagggatc
      481 aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc atatttccaa
      541 tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga agaaaatggg
      601 gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac attagaagat
      661 tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca ttactgggac
      721 aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca
      781 ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga gttagcacag
      841 caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag ctatatcgaa
      901 ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa agctatttct
      961 tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc agttgtctct
     1021 acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa
     1081 gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg
     1141 aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga tgatgattca
     1201 tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca
     1261 gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc gggtgatttt
     1321 gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt tactgatttg
     1381 tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata tgacgaagca
     1441 gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga tgatttccaa
     1501 aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa
     1561 ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg
     1621 gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg
     1681 aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca aagaaaaaag
     1741 catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa agctgtacat
     1801 accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt tccttgttcg
     1861 tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa
     1921 accgccgata atcagaaacg gcaatgacca taagaataga tcattagata attgaatgag
     1981 tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta gaaaagctac
     2041 tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt tcattatcta
     2101 ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt
     2161 atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa
     2221 caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt
     2281 tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg
     2341 tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat
     2401 ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag
     2461 ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca
     2521 tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca
     2581 ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt
     2641 tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca
     2701 tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata
     2761 gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac
     2821 atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat
     2881 tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat
     2941 tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact
     3001 catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa
     3061 gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa
     3121 cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac
     3181 gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga
     3241 aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga
     3301 tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc
     3361 ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg
     3421 aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa
     3481 cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc
     3541 tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt
     3601 ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag
     3661 gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt
     3721 caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac
     3781 aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa
     3841 gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa
     3901 tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag
     3961 gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc
     4021 tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct
     4081 ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac
     4141 gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa
     4201 ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt
     4261 tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag
     4321 caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc
     4381 agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat
     4441 gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca
     4501 taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg
     4561 aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg
     4621 tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga
     4681 acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat
     4741 tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa
     4801 tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga
     4861 agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac
     4921 attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa
     4981 cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga
     5041 acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc
     5101 tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa
     5161 agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg
     5221 aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa
     5281 atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa
     5341 gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga
     5401 tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc
     5461 ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag
     5521 tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc
     5581 gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc
     5641 gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg
     5701 cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc
     5761 aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat
     5821 ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata
     5881 acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt
     5941 atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca
     6001 gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg
     6061 caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa
     6121 aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt
     6181 taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg
     6241 tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg
     6301 agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga
     6361 agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta
     6421 tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta
     6481 tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa
     6541 acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc
     6601 tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc
     6661 agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat
     6721 ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct
     6781 gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag
     6841 gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa
     6901 acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata
     6961 tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca
     7021 cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt
     7081 tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt
     7141 agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag
     7201 cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct
     7261 tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta
     7321 cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc
     7381 tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt
     7441 ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga
     7501 aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt
     7561 cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt
     7621 tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc
     7681 cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa
     7741 agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct
     7801 agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc
     7861 atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca
     7921 gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga
     7981 aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc
     8041 cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg
     8101 gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac
     8161 agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc
     8221 ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt
     8281 catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa
     8341 aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg
     8401 aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc
     8461 acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca
     8521 agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat
     8581 ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa
     8641 aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata
     8701 ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac
     8761 ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt
     8821 ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta
     8881 gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa
     8941 actttttata tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg
     9001 tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg
     9061 ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta
     9121 ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa
     9181 acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct
     9241 gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa
     9301 ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac
     9361 aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg
     9421 gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac
     9481 tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac
     9541 tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta
     9601 ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat
     9661 attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca
     9721 gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg
     9781 ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg
     9841 aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa
     9901 cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat
     9961 ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta
    10021 gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct
    10081 gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt
    10141 gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc
    10201 tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca
    10261 aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg
    10321 cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat
    10381 caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct
    10441 tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa
    10501 gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc
    10561 aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat
    10621 gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg
    10681 gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg
    10741 tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt
    10801 gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct
    10861 gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc
    10921 tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt
    10981 ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa
    11041 tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat
    11101 ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat
    11161 aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt ccttacattc
    11221 ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag tagcgttgat
    11281 gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta tatgtgtgat
    11341 tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt ttctttattt
    11401 tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc aaacggcttg
    11461 gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc
    11521 cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc aaccctaaaa
    11581 gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta agtgccatca
    11641 ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag ttctttgtaa
    11701 atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa
    11761 gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct ttgatcaatg
    11821 caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt gcaacagtca
    11881 ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt tctttacggg
    11941 aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc ttaaaatcca
    12001 taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa aaaagaaata
    12061 catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg taaattaaaa
    12121 tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga tgggaatgaa
    12181 aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt
    12241 gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc agtctacaag
    12301 tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg gattgacagt
    12361 ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata ttgaagctgc
    12421 gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt actttcctgc
    12481 taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat tacaattatc
    12541 taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag ggttgaattt
    12601 gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta aattaatacc
    12661 aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg tccagttatt
    12721 tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg cgttgttagc
    12781 aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg aaatgaaacg
    12841 gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa cagtcgttta
    12901 tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg ccggtaattt
    12961 gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa agctggtcac
    13021 tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa ctgggatccg
    13081 cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc tgacaaagct
    13141 ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag tagcttgtat
    13201 tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg tgatgtggct
    13261 attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc
    13321 acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc tcggtgctat
    13381 tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg gtataggtgt
    13441 taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag aagagtaaaa
    13501 tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg tgctttttct
    13561 atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt agtgcttatg
    13621 tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat acccatttaa
    13681 atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa gaaatggggg
    13741 taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa
    13801 gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag gctggcagtt
    13861 atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag gttgtagcat
    13921 taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc caagataaag
    13981 tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt attcatacta
    14041 gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga gatatcggtg
    14101 gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt gacttaggga
    14161 tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc caagaggctg
    14221 cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat ctggcaccag
    14281 ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta gaaaagattg
    14341 ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca aatgcgcacc
    14401 gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat agtcgttgaa
    14461 ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat agaaacaata
    14521 gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga gactcgtgga
    14581 gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat cattatggaa
    14641 gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag
    14701 ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc
    14761 gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga ccttttagac
    14821 tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg
    14881 cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg
    14941 ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg aatatcgtga
    15001 aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt catttaaaaa
    15061 aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg tagaaactgc
    15121 gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg cattgacgga
    15181 acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc gtttgatccc
    15241 tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc aagatatttt
    15301 aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc cgttaaaagt
    15361 cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc ttgaatcaga
    15421 tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc
    15481 tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt atatggaagc
    15541 cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg tagattcagc
    15601 gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt
    15661 tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga ataacttgca
    15721 gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct tggaaacagc
    15781 tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg ccgcattaag
    15841 caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag
    15901 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg tttttcgttt
    15961 gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggttgtggg
    16021 cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct catgtttttg
    16081 agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg aacaaggaga
    16141 tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa ttcttatact
    16201 tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga cagttattag
    16261 taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga gagaaaaaat
    16321 gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga tgggggtcat
    16381 tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt tggcgtctca
    16441 tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg ttgcgctagg
    16501 tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag cattgtcaca
    16561 tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta tcgttttaaa
    16621 aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg tgatcggtgt
    16681 gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg gtaatatgcc
    16741 tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg ggattggtgg
    16801 gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa cactcagcaa
    16861 atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag agtcgattca
    16921 agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa ctctcatcta
    16981 gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag ctgcaccaac
    17041 atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact tgttctgttt
    17101 ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt
    17161 tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt tttgtgtaaa
    17221 gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact acggcttcag
    17281 gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata ccgaagataa
    17341 cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca ggaatacgaa
    17401 gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta aagaagttca
    17461 tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca taaacttctt
    17521 tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg tcagccatag
    17581 tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa
    17641 gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt gataatggct
    17701 catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc acacgttggc
    17761 gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga cctaagatct
    17821 ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt aatttcaagc
    17881 caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt tggaaaacca
    17941 tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg atatttagtt
    18001 ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac tttccacaac
    18061 cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta aaatcagtta
    18121 cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc atttctacca
    18181 taatacgttc cacactttct tttttagttt tattaactat ttaaagtata aatgaaagcg
    18241 ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc aatg
//
LOCUS       DAZBWR010000055        18194 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000055 DAZBWR010000000
VERSION     DAZBWR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.055"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..837)
                     /locus_tag="ACHGT5_001622"
     CDS             complement(13..837)
                     /locus_tag="ACHGT5_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN7217610.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(810..1490)
                     /locus_tag="ACHGT5_001623"
     CDS             complement(810..1490)
                     /locus_tag="ACHGT5_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN7217611.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1513..2451)
                     /locus_tag="ACHGT5_001624"
     CDS             complement(1513..2451)
                     /locus_tag="ACHGT5_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN7217612.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2696..3547)
                     /gene="ispE"
                     /locus_tag="ACHGT5_001625"
     CDS             complement(2696..3547)
                     /gene="ispE"
                     /locus_tag="ACHGT5_001625"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN7217613.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3701..6274)
                     /gene="mprF"
                     /locus_tag="ACHGT5_001626"
     CDS             complement(3701..6274)
                     /gene="mprF"
                     /locus_tag="ACHGT5_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN7217614.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6415..7221
                     /gene="proC"
                     /locus_tag="ACHGT5_001627"
     CDS             6415..7221
                     /gene="proC"
                     /locus_tag="ACHGT5_001627"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN7217615.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7300..8856)
                     /gene="rny"
                     /locus_tag="ACHGT5_001628"
     CDS             complement(7300..8856)
                     /gene="rny"
                     /locus_tag="ACHGT5_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN7217616.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9267..10316)
                     /gene="recA"
                     /locus_tag="ACHGT5_001629"
     CDS             complement(9267..10316)
                     /gene="recA"
                     /locus_tag="ACHGT5_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN7217617.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10425..11666)
                     /locus_tag="ACHGT5_001630"
     CDS             complement(10425..11666)
                     /locus_tag="ACHGT5_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN7217618.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11906..12484)
                     /gene="pgsA"
                     /locus_tag="ACHGT5_001631"
     CDS             complement(11906..12484)
                     /gene="pgsA"
                     /locus_tag="ACHGT5_001631"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN7217619.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12693..13616)
                     /locus_tag="ACHGT5_001632"
     CDS             complement(12693..13616)
                     /locus_tag="ACHGT5_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN7217620.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14354..15640)
                     /locus_tag="ACHGT5_001633"
     CDS             complement(14354..15640)
                     /locus_tag="ACHGT5_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN7217621.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15633..16901)
                     /locus_tag="ACHGT5_001634"
     CDS             complement(15633..16901)
                     /locus_tag="ACHGT5_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN7217622.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17153..17614
                     /gene="mscL"
                     /locus_tag="ACHGT5_001635"
     CDS             17153..17614
                     /gene="mscL"
                     /locus_tag="ACHGT5_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN7217623.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17673..18119)
                     /locus_tag="ACHGT5_001636"
     CDS             complement(17673..18119)
                     /locus_tag="ACHGT5_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7217624.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5190 a         3980 c         3181 g         5843 t
ORIGIN      
        1 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
       61 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
      121 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
      181 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
      241 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
      301 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
      361 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
      421 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
      481 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
      541 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
      601 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
      661 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
      721 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
      781 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
      841 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
      901 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
      961 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
     1021 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
     1081 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
     1141 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
     1201 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
     1261 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
     1321 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
     1381 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
     1441 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
     1501 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
     1561 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
     1621 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
     1681 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
     1741 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
     1801 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
     1861 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
     1921 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
     1981 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
     2041 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
     2101 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
     2161 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
     2221 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
     2281 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
     2341 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
     2401 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
     2461 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
     2521 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
     2581 aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat aaaaaactga
     2641 agaataccat tttaggattc ctcagttttt catcacctat tttacttctt atattttatt
     2701 gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta tatactctct
     2761 gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg
     2821 cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact ggatgccgct
     2881 ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga taatcttgct
     2941 ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc gtatctactt
     3001 ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca acccagcact
     3061 gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat gccgtacttc
     3121 cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc agttcttcta
     3181 acgaacagcc cagattccat aattggttga tccctcttag agcagctgca cagtctgtac
     3241 ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc ttgacaccgg
     3301 ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg ttccgttcat
     3361 ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt atttcttcaa
     3421 aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca tgatacccat
     3481 cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct ttttcaatca
     3541 attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca tagggaaaaa
     3601 taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa cagctttcac
     3661 agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct gtgagcactt
     3721 cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt agctgcatgg
     3781 tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc caatgatcga
     3841 catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta ccataattat
     3901 atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca acattagcta
     3961 atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca ataaacagat
     4021 tggctaacag atagtcagct aattcctcag attctttagg taagacccgc aacaaatcat
     4081 atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact ttttgatctt
     4141 tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt cccacagaga
     4201 agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca gcttgatgta
     4261 attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc aaacgttgat
     4321 attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca atccctgctt
     4381 cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag ctttctgata
     4441 tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac tgattcgttg
     4501 ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat ttgtttcctt
     4561 tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa aaataactgt
     4621 aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt
     4681 caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct aaatattgat
     4741 aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag aaccacacat
     4801 catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa aagcgtccgc
     4861 tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta gcatcaaaga
     4921 ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg gataaccaca
     4981 cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta cgtgtgattg
     5041 tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt ttgactttta
     5101 ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc actgtctgat
     5161 ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt gcagacagat
     5221 tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca cttacgagca
     5281 cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat cgattgattt
     5341 tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga acataataaa
     5401 acaaccgata atataatagc caaactactg ctacatcttg ccgaacaggc aattgactca
     5461 ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc gtcagcattc
     5521 ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata tctattctca
     5581 tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca aaggatgcca
     5641 gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt ctctttagat
     5701 aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag atccaatagc
     5761 ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga aacgaccata
     5821 cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa accatttttc
     5881 tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca ccaccaaaac
     5941 cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct tttctttcca
     6001 tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac aacaacatcg
     6061 gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc
     6121 ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg acttgatttg
     6181 ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta tgttccttta
     6241 tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa cttgtatact
     6301 tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta gacctgttct
     6361 ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga taacatgaaa
     6421 gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt attaaaaaaa
     6481 ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa
     6541 tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc atcacaatca
     6601 gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga agaactaaga
     6661 gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt aacagattta
     6721 gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc
     6781 agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt
     6841 cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc agaatcagat
     6901 ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat gctgatcgat
     6961 gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac tgagattgct
     7021 gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa
     7081 cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt tgccttagaa
     7141 gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga gaaagatctt
     7201 gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa aaaaagaaga
     7261 gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat aatcagttgc
     7321 acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt cttcttcgat
     7381 tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt cttctggctt
     7441 gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta caccggcgaa
     7501 gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta atgattcact
     7561 tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga cagagataac
     7621 agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta ctgggttttc
     7681 tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc
     7741 aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc
     7801 aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct tcaagacgtt
     7861 ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat ctggatgaag
     7921 tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac gttcatccat
     7981 ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc gtccatcttg
     8041 tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga atccggaaag
     8101 aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg tctcaagcgt
     8161 tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat tcggaagggt
     8221 aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga
     8281 taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag attcttttac
     8341 cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga catctcgtgc
     8401 gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt caatcatttt
     8461 ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg cttcttcttt
     8521 actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca gcgtttgttc
     8581 gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc tcagctcact
     8641 ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg cttctttttt
     8701 tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg attgtgcacc
     8761 agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa gaccgacaat
     8821 taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca tactatcttt
     8881 ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt caatatgcat
     8941 cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg taccccgtag
     9001 tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg atggttttta
     9061 aaataaatga cactagtttc atataggtaa acctttaaat tgagaaactt ttctattaaa
     9121 ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat aattaaataa
     9181 ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct actcttcaga
     9241 ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc ttcttgtcct
     9301 ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct aactagagca
     9361 gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt ttcccgtcct
     9421 tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac aatgtctttt
     9481 tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata catgacatct
     9541 acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat ctttgtacgg
     9601 ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc caaacggatc
     9661 gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc aaacatcacg
     9721 cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt gttgatcgaa
     9781 ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac atgtgagtca
     9841 cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc gactactacg
     9901 atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg ttctcctgtg
     9961 tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc atattgcgga
    10021 tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg tacttctgca
    10081 atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac ttcgatgata
    10141 cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga gccacttgga
    10201 attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc tttaccatag
    10261 ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc tgccaatacg
    10321 ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc atatcttttt
    10381 tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt tcttaaaagc
    10441 tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc agcacgatct
    10501 ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc gatccatact
    10561 gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa agctacactg
    10621 taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca ttctttgctg
    10681 actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc ttttgtttgg
    10741 agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat gtcgcctaat
    10801 gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg cttgttttct
    10861 ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc ataaaaatat
    10921 tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt ccctgcctga
    10981 acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc atatggtgcg
    11041 atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag tcgcgattca
    11101 ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg aaaacgttcc
    11161 tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg cggaccggga
    11221 agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag ccctgtacga
    11281 tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt tttggtcatt
    11341 tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt attttgtatg
    11401 agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct
    11461 aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat caactcttct
    11521 aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc tagatcagct
    11581 agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa aagttctgtt
    11641 ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta tacgctgtat
    11701 ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct tgtcacagct
    11761 ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc acaatataca
    11821 taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta gacggcaggc
    11881 tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg ctgtttttga
    11941 tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc atgatttgat
    12001 ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag ataatagcaa
    12061 ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact tctccatgtt
    12121 cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg atcgctacta
    12181 cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata actagcattt
    12241 tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc gcacgggcaa
    12301 tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca cctactagct
    12361 gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa ggtaaaccta
    12421 caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta tttggtaaat
    12481 tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt ttccaccgat
    12541 attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa ttgatctttt
    12601 tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt ttatacctac
    12661 aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag agtaaggttc
    12721 acattttttg gactctgatt gttttcccct ggattaactg gtacggattc tccattcgct
    12781 ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc tgtttccggt
    12841 aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata ggcattgtct
    12901 actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac aggcttggta
    12961 gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc tattgccatt
    13021 ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt ggtctgcgtg
    13081 ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga agaagctgtg
    13141 tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac gacaacgaca
    13201 atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa gcgacttgga
    13261 tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg ctgcggacgt
    13321 tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa atggtctcca
    13381 tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa tttaccaggc
    13441 aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat ttttgtaatc
    13501 tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa tttttctccg
    13561 atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg aagcatctgc
    13621 tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca cagctccttt
    13681 tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa tatccaataa
    13741 atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg gcaggctcac
    13801 gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca aaaaccggca
    13861 aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa cgctgatttc
    13921 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag
    13981 ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    14041 ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa taagccgaaa
    14101 tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca
    14161 cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa gttgattaaa
    14221 agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa gaataccata
    14281 aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa gacttttcct
    14341 cataaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa
    14401 atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat
    14461 caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg
    14521 aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt
    14581 ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt
    14641 tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga
    14701 attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag
    14761 aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt
    14821 ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg
    14881 taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac
    14941 gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca
    15001 tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa
    15061 acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc
    15121 ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca
    15181 tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct
    15241 tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat
    15301 tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag
    15361 cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa
    15421 acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac
    15481 catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct
    15541 tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat
    15601 aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa
    15661 aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg
    15721 ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc
    15781 aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc
    15841 agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct
    15901 aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg
    15961 acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct
    16021 ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag
    16081 accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc
    16141 agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt
    16201 tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact
    16261 gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt
    16321 gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg
    16381 actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag
    16441 gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg
    16501 ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat
    16561 aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca
    16621 ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat
    16681 aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgga
    16741 taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct
    16801 tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt
    16861 aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca
    16921 cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg
    16981 cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag
    17041 tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt
    17101 attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa
    17161 agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat
    17221 tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat
    17281 aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct
    17341 tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat
    17401 tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa
    17461 gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta
    17521 tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt
    17581 agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg
    17641 attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg
    17701 tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac
    17761 tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt
    17821 ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg
    17881 tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg
    17941 taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttg tccatcaatg
    18001 tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg
    18061 acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact
    18121 aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac
    18181 gaaccttacg aatc
//
LOCUS       DAZBWR010000056        17036 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000056 DAZBWR010000000
VERSION     DAZBWR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.057"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACHGT5_001637"
     CDS             complement(33..1076)
                     /locus_tag="ACHGT5_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7217625.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACHGT5_001638"
     CDS             complement(1237..1920)
                     /locus_tag="ACHGT5_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFN7217626.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACHGT5_001639"
     CDS             2115..3044
                     /locus_tag="ACHGT5_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN7217627.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACHGT5_001640"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACHGT5_001640"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFN7217628.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACHGT5_001641"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACHGT5_001641"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFN7217629.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACHGT5_001642"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACHGT5_001642"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFN7217630.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACHGT5_001643"
     CDS             5429..6232
                     /locus_tag="ACHGT5_001643"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN7217631.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACHGT5_001644"
     CDS             6316..7863
                     /locus_tag="ACHGT5_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFN7217632.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACHGT5_001645"
     CDS             complement(8160..8357)
                     /locus_tag="ACHGT5_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217633.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACHGT5_001646"
     CDS             complement(8433..8633)
                     /locus_tag="ACHGT5_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN7217634.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACHGT5_001647"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACHGT5_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACHGT5_001648"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACHGT5_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN7217635.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="ACHGT5_001649"
     CDS             complement(11515..12297)
                     /locus_tag="ACHGT5_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217636.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="ACHGT5_001650"
     CDS             complement(12362..13198)
                     /locus_tag="ACHGT5_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN7217637.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="ACHGT5_001651"
     CDS             complement(13216..14625)
                     /locus_tag="ACHGT5_001651"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN7217638.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14639..16462)
                     /locus_tag="ACHGT5_001652"
     CDS             complement(14639..16462)
                     /locus_tag="ACHGT5_001652"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN7217639.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         5048 a         3459 c         3190 g         5339 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtt
//
LOCUS       DAZBWR010000057        16771 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000057 DAZBWR010000000
VERSION     DAZBWR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.059"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHGT5_001653"
     CDS             complement(24..722)
                     /locus_tag="ACHGT5_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN7217640.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACHGT5_001654"
     CDS             complement(715..2067)
                     /locus_tag="ACHGT5_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN7217641.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACHGT5_001655"
     CDS             complement(2290..3690)
                     /locus_tag="ACHGT5_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN7217642.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACHGT5_001656"
     CDS             3851..4588
                     /locus_tag="ACHGT5_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN7217643.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACHGT5_001657"
     CDS             4713..5192
                     /locus_tag="ACHGT5_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN7217644.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACHGT5_001658"
     CDS             5189..5956
                     /locus_tag="ACHGT5_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN7217645.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACHGT5_001659"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACHGT5_001659"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN7217646.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACHGT5_001660"
     CDS             complement(6859..7461)
                     /locus_tag="ACHGT5_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN7217647.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACHGT5_001661"
     CDS             7567..7797
                     /locus_tag="ACHGT5_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217648.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACHGT5_001662"
     CDS             7788..8036
                     /locus_tag="ACHGT5_001662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7217649.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACHGT5_001663"
     CDS             8142..8726
                     /locus_tag="ACHGT5_001663"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN7217650.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACHGT5_001664"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACHGT5_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN7217651.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACHGT5_001665"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACHGT5_001665"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN7217652.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACHGT5_001666"
     CDS             10627..10806
                     /locus_tag="ACHGT5_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217653.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACHGT5_001667"
     CDS             10828..11853
                     /locus_tag="ACHGT5_001667"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN7217654.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12044..13288
                     /gene="glyA"
                     /locus_tag="ACHGT5_001668"
     CDS             12044..13288
                     /gene="glyA"
                     /locus_tag="ACHGT5_001668"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN7217655.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13441..14388
                     /locus_tag="ACHGT5_001669"
     CDS             13441..14388
                     /locus_tag="ACHGT5_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN7217656.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14522..15151
                     /gene="upp"
                     /locus_tag="ACHGT5_001670"
     CDS             14522..15151
                     /gene="upp"
                     /locus_tag="ACHGT5_001670"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN7217657.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15290..15532)
                     /locus_tag="ACHGT5_001671"
     CDS             complement(15290..15532)
                     /locus_tag="ACHGT5_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217658.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15653..15725
                     /locus_tag="ACHGT5_001672"
     tRNA            15653..15725
                     /locus_tag="ACHGT5_001672"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15686..15688,aa:Thr,seq:cgt)
     gene            complement(15844..16482)
                     /locus_tag="ACHGT5_001673"
     CDS             complement(15844..16482)
                     /locus_tag="ACHGT5_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN7217659.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16605..>16771)
                     /locus_tag="ACHGT5_001674"
     CDS             complement(16605..>16771)
                     /locus_tag="ACHGT5_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN7217660.1"
                     /translation="LENASFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGF
                     ARKFLQIKVV"
BASE COUNT         5722 a         2979 c         3355 g         4715 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag attacaaaac
    12061 gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag aacataattt
    12121 ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac aaggaagtat
    12181 cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg ggtgtgaatt
    12241 tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt
    12301 tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc tcgctttggt
    12361 agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc taacacatgg
    12421 atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg ttgatccgac
    12481 aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc aaccaaaatt
    12541 gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat tcagagaaat
    12601 cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag gattagtagc
    12661 agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta ccactcataa
    12721 aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa
    12781 aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg tgattgcggg
    12841 taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata gtgaacaaat
    12901 catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat
    12961 ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag gtataaatgg
    13021 aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa actcgattcc
    13081 ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc cagcaatcac
    13141 tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg tcaaagcatt
    13201 acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac
    13261 tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa cagaaaagtt
    13321 caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa tatcggtgat
    13381 gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg aggaaaagtc
    13441 atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct
    13501 catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga agaagtacaa
    13561 gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga tactggcgaa
    13621 tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa attaccagtt
    13681 attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca tgataaattg
    13741 atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata tagtcgatca
    13801 ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca acagctgaat
    13861 aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg agattcagta
    13921 ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg cctcccaata
    13981 tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga ttcctatcat
    14041 gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg ggaccaatat
    14101 acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc aaatataaat
    14161 gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt attaagagat
    14221 gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac acaagtacgc
    14281 ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg
    14341 cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc tttgcttgtc
    14401 cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag tttgactagt
    14461 caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga gagcttaaaa
    14521 aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa ctattattcg
    14581 ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat
    14641 ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa caccgatcac
    14701 tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc
    14761 aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag tgggacatat
    14821 cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca aacttccaga
    14881 agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag gcggatcagc
    14941 aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt
    15001 ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata ttgacatcta
    15061 tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg gactagggga
    15121 tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg tgacacaact
    15181 tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt tttcacagct
    15241 ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt caaatctttt
    15301 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    15361 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    15421 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    15481 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    15541 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    15601 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    15661 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    15721 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    15781 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    15841 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    15901 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    15961 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    16021 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    16081 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    16141 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    16201 tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat tgataaaaaa
    16261 atatttggct atatttttga caggagcgac aggtttcgag aacatttttt tcgtatcttt
    16321 tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat
    16381 aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat
    16441 ccctttttta agttctattt tttttcctaa aatgttattc atgataataa ctcctactat
    16501 tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat
    16561 ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt
    16621 tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga
    16681 tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa
    16741 tcagtatcaa aaaagctagc attttctaaa t
//
LOCUS       DAZBWR010000058        16702 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000058 DAZBWR010000000
VERSION     DAZBWR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.060"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..2216
                     /locus_tag="ACHGT5_001675"
                     /pseudo
     CDS             157..2216
                     /locus_tag="ACHGT5_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2299..2868
                     /locus_tag="ACHGT5_001676"
     CDS             2299..2868
                     /locus_tag="ACHGT5_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7217661.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3043..3849)
                     /locus_tag="ACHGT5_001677"
     CDS             complement(3043..3849)
                     /locus_tag="ACHGT5_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217662.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3987..5501
                     /locus_tag="ACHGT5_001678"
     CDS             3987..5501
                     /locus_tag="ACHGT5_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN7217663.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5904..6887)
                     /locus_tag="ACHGT5_001679"
     CDS             complement(5904..6887)
                     /locus_tag="ACHGT5_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN7217664.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6892..7539)
                     /locus_tag="ACHGT5_001680"
     CDS             complement(6892..7539)
                     /locus_tag="ACHGT5_001680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN7217665.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7624..8256)
                     /locus_tag="ACHGT5_001681"
     CDS             complement(7624..8256)
                     /locus_tag="ACHGT5_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN7217666.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8272..8823)
                     /gene="coaC"
                     /locus_tag="ACHGT5_001682"
     CDS             complement(8272..8823)
                     /gene="coaC"
                     /locus_tag="ACHGT5_001682"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN7217667.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8820..9557)
                     /locus_tag="ACHGT5_001683"
     CDS             complement(8820..9557)
                     /locus_tag="ACHGT5_001683"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN7217668.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9554..10543)
                     /locus_tag="ACHGT5_001684"
     CDS             complement(9554..10543)
                     /locus_tag="ACHGT5_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN7217669.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10801..11343
                     /locus_tag="ACHGT5_001685"
     CDS             10801..11343
                     /locus_tag="ACHGT5_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN7217670.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11360..12445
                     /locus_tag="ACHGT5_001686"
     CDS             11360..12445
                     /locus_tag="ACHGT5_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217671.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12457..13263
                     /locus_tag="ACHGT5_001687"
     CDS             12457..13263
                     /locus_tag="ACHGT5_001687"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217672.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13264..14091
                     /locus_tag="ACHGT5_001688"
     CDS             13264..14091
                     /locus_tag="ACHGT5_001688"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217673.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14088..15161
                     /locus_tag="ACHGT5_001689"
     CDS             14088..15161
                     /locus_tag="ACHGT5_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217674.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15589..>16702
                     /locus_tag="ACHGT5_001690"
     CDS             15589..>16702
                     /locus_tag="ACHGT5_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN7217675.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLES"
BASE COUNT         5392 a         2984 c         3402 g         4924 t
ORIGIN      
        1 acataagtta ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta
       61 atagtccatc ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact
      121 tcatgtttag acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa
      181 caactaaata aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac
      241 gttaagttta tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta
      301 cccgttcagg caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact
      361 gattccccta tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct
      421 gcattgactt cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt
      481 cctaacaaaa cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc
      541 gatatgactg atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat
      601 gctttgacag atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat
      661 tattcacagt ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct
      721 gatctggtag aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt
      781 tcaaacaatg ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc
      841 tattttgatc caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct
      901 gatcaaacag ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct
      961 aaagatcttg ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc
     1021 aaaatgaacg ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat
     1081 atagactcga aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct
     1141 gctgcttcat cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa
     1201 gcagaaacac atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt
     1261 tctgatcgag tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct
     1321 atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt
     1381 gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga aattaccttt
     1441 acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca
     1501 aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc
     1561 gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg
     1621 accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa
     1681 gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa
     1741 gaacaaaaaa gacaagaaga aagcttgtca gaaaaaaaca acaaatggct catgcagttt
     1801 ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa
     1861 tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat
     1921 tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta
     1981 aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt
     2041 caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa
     2101 cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg
     2161 caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta
     2221 tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag
     2281 acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca
     2341 agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt
     2401 tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa
     2461 acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt
     2521 gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct
     2581 tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca
     2641 gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct
     2701 accctccata cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt
     2761 gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat
     2821 ccagattatg aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa
     2881 ataagtcctc agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg
     2941 agttaatact atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac
     3001 aaaagtattg tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt
     3061 aggaataaaa ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc
     3121 aaatacactg aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat
     3181 gattcccacg tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt
     3241 ttgactttca atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt
     3301 acttaactcc acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag
     3361 taatttatct gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca
     3421 gaaggaaaca actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag
     3481 caaataaaca agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa
     3541 agtatcccac attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact
     3601 aaaaataaaa gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt
     3661 ttccagaaaa taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc
     3721 ccagtgttgg ttccagacct gatccactag cataataacg acgatgatac gtaataaaat
     3781 atagattccg tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa
     3841 atgattcata aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta
     3901 ccaaagctat atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat
     3961 agtataagga gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg
     4021 ttgcacttct tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt
     4081 ctagtacgaa ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc
     4141 caaaagaaat gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg
     4201 cagcagctat cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg
     4261 ttgccggagg aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc
     4321 aaaaagaaga aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg
     4381 gtggaggaac gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg
     4441 tcgattggct agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga
     4501 gtgaaaagag gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg
     4561 ggttattgaa taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag
     4621 acattaagaa aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac
     4681 caaaagaggt caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag
     4741 aatttattga aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat
     4801 caactggaga tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata
     4861 aaatacaaat tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc
     4921 gcggagaagg agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata
     4981 caagggataa agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt
     5041 ttgaccaagg cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg
     5101 tcacaactgg aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac
     5161 tagcaaaaac agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca
     5221 accgtgccac agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg
     5281 caccaggaat ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag
     5341 ataaagaaaa ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac
     5401 atggaaacaa ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag
     5461 cgggcataca agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa
     5521 gaggttgtga aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta
     5581 gtgaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca
     5641 gcttcttatg tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     5701 tttattcgga aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
     5761 acgtataaac ggcggaagca gaagaaattc cttcggaaat aagctgaaat tcacaaaaat
     5821 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     5881 cctctttttt gatcagcatg attttattca gttatcttca aaatgatttt tccgatgttt
     5941 ttgtttgctt ccatatattc atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca
     6001 atgattggcc gaatcttgcc attttctaga aaaggcccag ctttttgcat aaaagtttgt
     6061 gcgagctctg ctttatactg atcacttcta ggtgtcaata atgtaccaag cagttggaca
     6121 cgtttcccaa tcaatacacc aaggtctatc tcaggcacga tggtcccacc tagcatacca
     6181 atcaagaccc aacgtccatc aacggctata gaagctaaat tcttttccca gtaagaagcg
     6241 ccgatgaaat ccaaaataac atcgactccc cgaccttcag tgatttttcg tacctcttct
     6301 tgaaagtctt gttttttata gttgatcaat tcatctgcac caagttcttt acaaaaggcg
     6361 agtttttctt ctgatcctgc agtaacgatg attttcgcgt gagaaagctg tctagctaat
     6421 tggatcgctg ctgttcccac accacttgct cctgcatgga tcaagacagt ttcgccttct
     6481 tgtaattttc caagccagta aagtgtttga taagcagtca aaaaaacttc tgagattcca
     6541 gcagcagaaa catagtctag cgtatcaggg agaaggattg cacgatcggc aggcatcaca
     6601 gcatattcgg cataaccacc tagattgact aatccgtata cttttgtccc aggagtaaaa
     6661 gtggagatat cacttttatt ctcaactacc tctccagcta cttcgactcc caaaataggg
     6721 tagggaggtt tcaaagagac tgattctctt ttcataatat ctgttcgatt gacagctgct
     6781 gtatggactt tgatcagtaa ttcgccttgt tttggtttag gaacaggcac ttcaatttgt
     6841 tctaatacat ctacagaacc aggatgcttg attgaaatgg cacgcatgga tctattcctc
     6901 ctttgcccat tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa gtactagagt
     6961 cgtacgagaa atatccgttt cttccactag agcggcaagt acagcagcaa ctgtagagcg
     7021 agtgatcgtt tttgatgcct tctcattagg cttagttaat tgaatggaag taacttcttc
     7081 atcgttagtc aatgtgactg gttgaagaat gacaaaatct aagttagttc gtttcagcca
     7141 ttcatctgca taatgtttag taatataata atctgtcatg gactcaggcc attttgctgg
     7201 ttcatctgca taaacagcac tgaccatcag atagcgagag acatgcgcat tttcagctgc
     7261 gatcactgtt ttgattgcgc catccaagtc tacttgcaga agattctttc cttgagagcc
     7321 ggcagcaaaa atcaatacat cgatgccttt gaatgcttcg gtcatttttt ccacggacca
     7381 agttaaatca aaagagacat acgaaacatt ctcttttttt actaatgttt gggaagcaac
     7441 atctctcacc cctgcaaaaa tttcatggga acttggagct aaatctttta tcaggtgtcg
     7501 gccaatttgt ccgtttgcac ctacaacaaa aattttcatt tggatcatcc tttcgacgtc
     7561 aaatctttcc attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga
     7621 ctatcagtta gtaagagaag ggtgcttgaa gtatcttaac aagacattgc atactgctgc
     7681 agtcgcaaga cctgcgacga ttccttcagg tataccactt gtaaaaataa ccgctagaat
     7741 agctgaataa acagcattta aatttgttcc gatgactttg gcataatctt gctggaataa
     7801 aaagtagatc atattcatca ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa
     7861 gaataaagaa acggaatctg ctttttgttt cattagtttg cgcagacctt tataaacgaa
     7921 ataaggaacc accccaatca agatgcgagg aataaacgcg atcagcaagg ctttccagct
     7981 tccatgttct gttccgatga ctgggataaa cggagaaaag acaaaagaca aaggtgtttg
     8041 gataactgtg ctacggatca tgctgatcag tccaaatgta ccgccaagaa aagcacctag
     8101 tcttggtcct aaaacgatcg aagcaatgat tacaggaata tgcatggttg ttgcattgat
     8161 tggaccgata gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa
     8221 catcgcagtt aatgtaaagt tttttgtatt tttcataagt actccttttt tctaagcatt
     8281 tctttcatta agagtcagca tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc
     8341 ttttccaaaa tctccacaag ctaaaagaga ttctcttggt tcaatttcct gatagccgac
     8401 agatctcaat gtttccagat tacgctgagt gatcgggttt tgatacatat tcgtgttcat
     8461 ggcaggggca atcaattttg gtgttcccac tggaagcgcc attgcgactg ttgaaagaag
     8521 atcgtctgca atgccgtttg ctagcttgcc gataatgttt gctgtagcag gcgcgatgag
     8581 aaacaagtca gcttgttttg ctagttcgat atgattgatt acctgcggat ttgattcttg
     8641 catgacgtct gtatggactg cgcgcttcga cagagattgc aaggttaatg gcgtgataaa
     8701 atgtgtgctg cttttagaca taatcacatc tacctgggca cctaatttga cgaattgact
     8761 tgtaagatca gcgcttttgt aagcagaaat acttccagta acgcctagta agatcttttt
     8821 cattttgatt cctccagtct aattttctca gtaatcaatg cagcaatctc agatttactt
     8881 tgagcttctt ccactgttcc atctttagat aagagatagc cgtggtgtgt ctcgccatgg
     8941 atttccgtca aatcatttgc gaaaataaaa tcagttttgt tatttatcag actatttttt
     9001 gcgactgcca gaagagattc ctttggtaca tctactaata gtttgaaacc aatgattttt
     9061 gcttttggct gccattcacg tagataagaa atgatttttg gtgttttctt caaaacgagc
     9121 acaagatgct cggtgttaga agagatcttt gaaacagtgg tatctgtctg ttcattttcc
     9181 gcaaaccaat tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt
     9241 tcttctgaaa aactaaaagc aggagtaaaa tcactgactg ccatactgtg gataatcgca
     9301 tcgtagtgtt cttttttcgt tagttcttct aattgtaaaa ataagtccag tgcagattcg
     9361 atcgtataaa tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg
     9421 taatcgattg tggtatccgg attggcagct aaataatcag ctaatgcttg ccctaatcgt
     9481 cctgtcgaat gatttgtaat agcacgtact tgatcgattt tttcagtcgt tcctccagct
     9541 gtgattagaa tcttcattta tggtcaccta catttactag tgttcttcct tggtgtgttc
     9601 cagcaagtag tttagatgct gtttcaggca attgatccag agtaatctct tggacgacca
     9661 gttcatctgc aatctgcaaa tccgccatct gttgccataa agaaagacgt tgatctattg
     9721 gtacattgac agaatcaatt ccaatcaact ggatgtttcg cagaataaaa ggcaagacag
     9781 tcgcgtttag tttgattccc ccagcattgc cacataaagc tactgcaccg ttataagaga
     9841 tcaatggaag aagactgctt agctgctcgc cacctactgt gtcgatgaca tagtcgattt
     9901 gctgttttcc taaagctttt gttgtctctg gcagaaattc atcaggtcgt acgacttgca
     9961 aagcacctag actttttagc catgtgacag actcttcttt tctggaaaag gtaatgattg
    10021 actggaatcc tagtttatgt aacagggcta tcgctgtact gcctacaccg ccagaagcac
    10081 cagtaacgac aactttttta tcggaggtca taccttgacg gattaaagcg ttcacacaca
    10141 acatagcagt aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc atattttttg
    10201 gtagaggaac taaccagtct cctggtactt tttgatattg gctataaccg cctggatggg
    10261 tgacgccaag cccaaaacca gtagcaagta caagatcttc ttttgaaaaa cgtggattgt
    10321 tactttggac gacagttccg gataaatcaa tccctggagt catcggatat gttcgtatca
    10381 cctgaccaga ctcgctcatt gccaatgcat ctttataatt gacatcagaa taggcaattt
    10441 ttaccaagac ttcattttca gccaactcag taaatggcag atcactgatt gctgtatgga
    10501 aaacaggttc ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag acactccttt
    10561 taaaattttc ttttatcata ccataaagcc tactctttta ttcttgttct tccgtaaatt
    10621 tagacaaaaa ttaggtgaaa aataaagaat caaacaacta gaatttattc caaaagataa
    10681 agaaatattc agcaaaaaaa attttacaaa gggttgcctt tttcaataaa tttcgtatac
    10741 taatacgagt ggtagtaaac aggaagttta ctattagaaa acttcgattc ggaggaaatt
    10801 atggaaattg gtgagaagtt aagaaatttg cgcatccaaa agaatttgac acaagaagaa
    10861 ctaggcgaac gcacagattt gtcaaaaggg tatatttccc agttggaacg agatttaagt
    10921 tcaccatcaa tggaaacatt cttttctatt cttgaagtct taggtgtgac tccagaagaa
    10981 ttttttcatc aagaagcagc gaatctgcaa gtggtttatt caaaagaaga tcatacggtt
    11041 tatttcgatg aagaaaacgg gtatgaattg gaatggctcg tggctgattc gaatgaaaaa
    11101 gaaatggagc ctgtcttgct gacttttgaa ggtgcaggag aatataagac gtttgaacct
    11161 tcattatctg aaacgtttat ttttgtatta gaaggggaat tagagttaag cttaggagag
    11221 acggtctatg tggcaaaagc aggacagtcg atctattatc aagcgacaaa acaacatcaa
    11281 ttaaggaatc atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca atcatatttg
    11341 taatataggg ggtaaaaacg tggggaaacc aatcatttca tttgatcaag tagtgaaacg
    11401 ctatgatgat gaaacagtat taaaaaaagt aagttttgag atcgaacaag gaaaatttta
    11461 tacactgctt ggtccttccg gctgtggtaa aacaacgatc ctacgtatta tagctggatt
    11521 caccgaagct acagaagggg acatctattt tgaagggaaa cggttgaatg atttgccagc
    11581 taataagcga caagtaaata cagtcttcca agattatgca ctttttcctc atatgaatgt
    11641 ttttgataac gttgcttttg gattgaagat caagaaaatg tccaaatcag atatcgaaaa
    11701 gaaggtaaaa gatgctttgc gtatggtcca gcttcctgga tacgaaaaac gggagattag
    11761 tgaaatgtct ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg taaatgaacc
    11821 gaaagtattg ctattagatg aaccgttatc ggcgcttgat ttgaaactac gtacagatat
    11881 gcagtatgaa ctgcgggatc tgcaacaacg attaggaatc acgtttattt ttgttactca
    11941 tgaccaagaa gaagcgttgg caatgagtga tgagattttt gtcatgaata aagggaaaat
    12001 cgtccaaagt ggtacacccg tagatattta tgatgagcca atcaatcatt tcgttgctga
    12061 ttttgttggg gagagcaata tcgtggacgg tgtaatgatc gaagataatc tagttgagtt
    12121 tgttggtaag caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt
    12181 tgttttacgc ccagaggatt tgacgatcac gacaccagat aaaggaaaac tagtcgtaac
    12241 ggtcgatact cagctgtttc gtggtgtcca ttatgaaatc atctgttatg atgaacagca
    12301 aaatgaatgg atggtccatt caacgaaaaa agctaaagaa ggaagcaaag taggattggc
    12361 ctttgagcca gaagatatcc atgtcatgcg tttcaacgag tctgaagaag aattcgatgc
    12421 acgtcttgac agctacgaag aatagggggg acaagaatga aaacaatgcg caggatctat
    12481 tccgtccctt atattttgtg gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt
    12541 tatcaatctt tttttgatat gaatgggcaa ttcacgctga ataattatct atcttatttt
    12601 acgtcgggga catcgctttc tatgacgatc aattctgtat ggtatgcatt tttgatcacg
    12661 tttttcacgc tgatcatcag ttacccgact gcttattttt taacaaaact gaagcacaag
    12721 caattatggc tgatgctgat tattttgcct acatgggtga atctgctgct taaagcttac
    12781 gcatttattg ggattttcag tgtgcacggc agcgtgaatc atttctttga ttttatcggg
    12841 atcgggccga aacagatatt attcacagac ttcagttttc tgttcgtggc aacgtatatc
    12901 gagattccat ttatgattat gccaattttc aatgcattgg aagagatgaa tccttctttg
    12961 atcagtgcta gtagagattt aggcgcgaat agctttgaga ccttccgacg agtgattttt
    13021 ccgttatcat taaatggtgt gaaaagtggt gtccaagcag tatttattcc ttctctttct
    13081 ttattcatgc tgactcgtct gattggcgga aatcgggtga tcactttagg aacggctatc
    13141 gaacagcatt tcttggttac acaaaactgg gggatgggtt ctacaatcgg tgtgatttta
    13201 atcattgcta tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg aggtcgctca
    13261 taaatgaaaa aatttaaatg gtcgtatctt tatttaggga tcgtcttttt attcttatat
    13321 gcaccgatct tctatctgat tttttattcc ttcaatgaag gaggaacgat gaacagcttt
    13381 acaggattta cttgggaaaa ttatgaagct gtttttgaag atacacgttt gatcattatc
    13441 gtattgaata cgttcatgct ggctttctta tctgcattga tcgccacgat catcgggaca
    13501 tttggggcta tgggaatcta ttacacgaaa aaacgacgtg cacggacagc gttgttgagc
    13561 atgaataaca tcttattagt ctctcctgat gttattatcg gtgctagttt cttgattttc
    13621 tttacactac taggattcgg attaggtttt cagagtgtat tgctttccca tattgctttc
    13681 agcattccaa tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg
    13741 gtcgatgcgg ctagagacct tggtgctaat aatttccaag tagtcaaaaa tatcattttg
    13801 ccttatcttt ctccaggaat catagctgga tattttatgg catttaccta ttctcttgat
    13861 gattttgcgg ttacattttt cgtcacagga aatggcttta cgacgctctc agtagagatc
    13921 tattctcgtg caagacaagg aatcagttta gaaatcaatg cgctaagtgc gttagttttc
    13981 ttgttctcca tgatattggt cattggatac tactttatta gtaaagaaaa ccgtccaaaa
    14041 cggctgaaga aaaacagaag agtacgcgag ggggtggcac ggctgcgatg aaaaaattac
    14101 aatctctgtt tttaggtatt ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac
    14161 ttgaaaaaac aagtggcatg agtggagcaa aggtacttac catctataac tggggagatt
    14221 atatcgatcc tagtttgatc acgaaatttg aaaaagaata tggatacaaa gtgaattatg
    14281 aaacatttga ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg acaaactacg
    14341 acatcgctat accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc
    14401 cattagatca ttcgaagatc aaaggattaa ataatattga tccacgattt ttagacttgg
    14461 attttgatcc agataatcag tattcgattc catatttttg gggaacttta gggatcgtat
    14521 ataacgataa attctttgat gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg
    14581 aattaaaaga cagtttgatg ctgattgacg gtgcacgaga agttatgggg ctttctttga
    14641 atagtttagg ctattctttg aatagtaaga atatggcaca gctgacagaa gcagcaaata
    14701 aattgaatca tttaacacca aatgtcaaag caatcgtggc agatgagatc aaaatgtata
    14761 tgatcaatga agaagcctcc gtggcagtga cattttctgg cgaagctgcc gatatgatgt
    14821 gggaaaatga acatttacat tatgtgatcc caccagaagg atccaatctt tggtttgata
    14881 atatcgttat gccaaaaacc gtgaaaaata aagaaggtgc ctatgatttc atcaacttta
    14941 tgctagaacc ggaaaatgcg gcacaaaatg cagagtatat tggttattct acaccaaatg
    15001 aagaagcaaa agcgttattg ccaaaagaca tttcgtcgga cgaacaattc tatccatctg
    15061 atgacacgat ctcccatctt gaagtctaca aagatctagg atcgaaatac ctaggcatct
    15121 ataatgacct attcttagaa ttcaagatgt ataggagata atggcaaaac tcagtaagaa
    15181 tggggactta tcaacgttct aaagagctaa aaatcgtcct ttttctatct gagttttcca
    15241 atcttctacc aaaagcggat gaaacgccgc tttttttgtg ttattttatt ttttctacca
    15301 cgtttttcta ccagagaaaa gtggacaaaa ccattgtaaa atagggcccg agacgggcta
    15361 gtaaatctaa gattgaaaaa gttacagaag gataaagtaa ttggaaacta tgcaaatata
    15421 cttaaccctt ctgtaatcta ttaataaaac taatatatga atccagttca atatttgtag
    15481 ctatgaaaaa acataaaaat catattagat aaaaactaaa ttggtagata aattattttg
    15541 tcagatatct gaggcaaata ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa
    15601 tatagaatag acatattgca gccgatagtt tattatgctt ttagacacat taaactggag
    15661 gaaaaaatga aaaagagcgc taaagatcga gtttttgatg agctgatacg actaacaggg
    15721 aatatgtctt ctgtcactgc cgagaaaatt tcgaagaatc taggtatctc tcgtcagaat
    15781 gccagccatt atttgacgag attagtggaa gataaaaagg tcgagaaatt accgggaaag
    15841 cctgttttgt ggaagccttt agatgaatat gcggttgtag ataatacgga gcaaataaac
    15901 gaagcctttc attctgtagt tggtcatgac ggaagtctga gagaggtcat tcaaaaatgt
    15961 atagccgctg tcaaatatcc gcctaacgga ctcagtgtat taataaatgg agccacgggt
    16021 gttggaaaaa gttttttagc cacaaaaatt tttgaatacg ctatccatga gcaaattatt
    16081 gaaaaagatg caccatttgc tatcttaaac tgtgccgatt atgcggacaa tcccgaactg
    16141 ctttcggcaa cactgtttgg atacaagaag ggggccttta cgggggctga aaaggatacc
    16201 gatggtttat tggcaacggc caacaacggc tatctttttc tcgatgaggt ccatcgccta
    16261 tcgaaagaaa atcaagaaaa gctctttcta tttattgata ctggaaacta tcgtccagtc
    16321 ggtgaaaatg taaattggca ttctgctaag gttcgtttta ttttcgccac gacggaaaaa
    16381 ggtgaaaatt atttattaga cacctttgat cggcgtattc aaattagtgt tatgttgcca
    16441 acttttgaag atcgccccat tcgagaacga ttagagctga ttcaattatt ttttcaaaat
    16501 gaagcggatg ttttacaaaa agatattatt gtttcaagag aagccttgtc tttaatgtta
    16561 tctcatccct ttagtgggaa tattggtaag ctgaagaata tcattaaaat cagttgtgcg
    16621 gatgtgtata gtcgaacaca agaagaacca ttaacgattg ggaaaaatga gatcttgatt
    16681 cagttaaata tgctggaatc cg
//
LOCUS       DAZBWR010000059        16632 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000059 DAZBWR010000000
VERSION     DAZBWR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.061"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..282
                     /locus_tag="ACHGT5_001691"
     CDS             133..282
                     /locus_tag="ACHGT5_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN7217676.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            293..1807
                     /gene="dltA"
                     /locus_tag="ACHGT5_001692"
     CDS             293..1807
                     /gene="dltA"
                     /locus_tag="ACHGT5_001692"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN7217677.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1804..3009
                     /gene="dltB"
                     /locus_tag="ACHGT5_001693"
     CDS             1804..3009
                     /gene="dltB"
                     /locus_tag="ACHGT5_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN7217678.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3054..3287
                     /gene="dltC"
                     /locus_tag="ACHGT5_001694"
     CDS             3054..3287
                     /gene="dltC"
                     /locus_tag="ACHGT5_001694"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN7217679.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3290..4558
                     /gene="dltD"
                     /locus_tag="ACHGT5_001695"
     CDS             3290..4558
                     /gene="dltD"
                     /locus_tag="ACHGT5_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN7217680.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4699..5781
                     /locus_tag="ACHGT5_001696"
     CDS             4699..5781
                     /locus_tag="ACHGT5_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN7217681.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5781..6584
                     /locus_tag="ACHGT5_001697"
     CDS             5781..6584
                     /locus_tag="ACHGT5_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN7217682.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6654..7394
                     /locus_tag="ACHGT5_001698"
     CDS             6654..7394
                     /locus_tag="ACHGT5_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN7217683.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7405..8409
                     /locus_tag="ACHGT5_001699"
     CDS             7405..8409
                     /locus_tag="ACHGT5_001699"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN7217684.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8454..10298)
                     /locus_tag="ACHGT5_001700"
     CDS             complement(8454..10298)
                     /locus_tag="ACHGT5_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217685.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10509..11027
                     /locus_tag="ACHGT5_001701"
     CDS             10509..11027
                     /locus_tag="ACHGT5_001701"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN7217686.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            11155..11838
                     /locus_tag="ACHGT5_001702"
     CDS             11155..11838
                     /locus_tag="ACHGT5_001702"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN7217687.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12147..12220
                     /locus_tag="ACHGT5_001703"
     tRNA            12147..12220
                     /locus_tag="ACHGT5_001703"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12181..12183,aa:Arg,seq:tct)
     gene            12379..12738
                     /locus_tag="ACHGT5_001704"
     CDS             12379..12738
                     /locus_tag="ACHGT5_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN7217688.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            12861..14492
                     /locus_tag="ACHGT5_001705"
     CDS             12861..14492
                     /locus_tag="ACHGT5_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN7217689.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(14554..15195)
                     /locus_tag="ACHGT5_001706"
     CDS             complement(14554..15195)
                     /locus_tag="ACHGT5_001706"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN7217690.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    15381..15457
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15477..15785
                     /gene="rplU"
                     /locus_tag="ACHGT5_001707"
     CDS             15477..15785
                     /gene="rplU"
                     /locus_tag="ACHGT5_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN7217691.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            15812..16156
                     /locus_tag="ACHGT5_001708"
     CDS             15812..16156
                     /locus_tag="ACHGT5_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN7217692.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            16169..16462
                     /gene="rpmA"
                     /locus_tag="ACHGT5_001709"
     CDS             16169..16462
                     /gene="rpmA"
                     /locus_tag="ACHGT5_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN7217693.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         5299 a         2741 c         3415 g         5177 t
ORIGIN      
        1 ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa acggtttctg
       61 taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga ggttatatga
      121 ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg gggaaaattc
      181 atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct gtatcactat
      241 aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga gtatggaaat
      301 caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc gagttgctta
      361 tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg cacttgctta
      421 ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca atttggagtt
      481 tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt tgccgattga
      541 agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac catctatggt
      601 catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa caaaaaaaga
      661 atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg taaaaggtac
      721 agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag gcgtacaaat
      781 cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg ggatcaagcc
      841 tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat tttctattta
      901 tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg ttcaagactt
      961 tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt ctactccatc
     1021 atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc cggaattgga
     1081 catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc tacttgatcg
     1141 attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg tagctatttc
     1201 aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga ttggctacgt
     1261 aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac
     1321 gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa
     1381 gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag gggatgcagg
     1441 tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc aggtcaaatt
     1501 acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag acaaccgaat
     1561 caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc tggttgcttt
     1621 tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat ctatcaaaga
     1681 acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt atgtcgatct
     1741 gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc
     1801 tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt tcttttagcc
     1861 attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt accagtttat
     1921 caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta ttggcaacag
     1981 ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg ctattttgtt
     2041 tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt agcgataatt
     2101 ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc gttacttggt
     2161 tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga aataagagat
     2221 ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt ctttccaaca
     2281 atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct ccatccgcct
     2341 gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt
     2401 ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt agtgggacgc
     2461 attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta cgtgtacagt
     2521 atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac aagctacatg
     2581 atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa tatcaaagaa
     2641 ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt atatatgcga
     2701 ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc
     2761 ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg gtactacatc
     2821 gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat ccgatttaag
     2881 aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc cgtatttttg
     2941 acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga taaacttttt
     3001 tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg aaaatggaag
     3061 aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg
     3121 atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa ttattagtcg
     3181 agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga gaggattggt
     3241 gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa
     3301 actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg tgttttttac
     3361 ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct cttcttccat
     3421 gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg aaaaacaata
     3481 tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc cgtctgttct
     3541 ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg ggacacaatc
     3601 gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt
     3661 ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt actttaatac
     3721 gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag taacgcctgc
     3781 tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga atgatacgtt
     3841 gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat ctttgaatca
     3901 gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca atattgggtt
     3961 gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac aataccaaga
     4021 aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga ataatccatt
     4081 cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag agctcaaaca
     4141 gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc agctggtctt
     4201 ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa
     4261 atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa
     4321 atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac
     4381 caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca
     4441 acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga
     4501 tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag
     4561 aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata
     4621 aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat
     4681 agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct
     4741 gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa
     4801 ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct
     4861 ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca
     4921 aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc
     4981 atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt
     5041 ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag
     5101 gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt
     5161 cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta
     5221 ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat
     5281 ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta
     5341 ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta
     5401 gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta
     5461 ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg
     5521 aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct
     5581 ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc
     5641 attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat
     5701 ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa
     5761 aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag
     5821 cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa
     5881 tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca
     5941 tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg
     6001 cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat
     6061 tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag
     6121 tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga
     6181 gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag
     6241 attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca
     6301 tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg
     6361 atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta
     6421 cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt
     6481 tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac
     6541 aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat
     6601 tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat
     6661 taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt
     6721 tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat
     6781 tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg
     6841 atcatattgc tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc
     6901 cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga
     6961 cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc
     7021 cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg
     7081 tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt
     7141 tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa
     7201 atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta
     7261 acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa
     7321 atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag
     7381 tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga
     7441 atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg
     7501 ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa
     7561 atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc
     7621 ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg
     7681 gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg
     7741 ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt
     7801 tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc
     7861 gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca
     7921 aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa
     7981 aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta
     8041 gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat
     8101 cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc
     8161 aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca
     8221 gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct
     8281 gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc
     8341 agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta
     8401 ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca
     8461 ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc
     8521 tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt
     8581 tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca
     8641 ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt
     8701 cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc
     8761 aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag
     8821 gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc
     8881 acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt
     8941 tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc
     9001 tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca
     9061 taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg
     9121 tcggtattta agatgtgatc gattagattg tagctgataa taacgattag aatgataata
     9181 tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa
     9241 atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg
     9301 ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta
     9361 gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc
     9421 agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag
     9481 taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa
     9541 tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat
     9601 acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga
     9661 cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca
     9721 gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt
     9781 ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag
     9841 actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag
     9901 agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga
     9961 tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt
    10021 tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg
    10081 tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt
    10141 atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt
    10201 cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg taaataaaaa
    10261 gattcttttt tattatatat gttattaaaa aaagacataa aggtattcct ttctaaaact
    10321 tgtaatgtta gggtaaacgt tttttaaact aacgtcaatt atttataata aaaaatgaga
    10381 taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat ttttacagct
    10441 ataatttgaa aaaatttatc gttattatat gattaagaag gattgaataa agaaagaagt
    10501 tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg ttgattatat
    10561 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
    10621 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
    10681 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
    10741 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
    10801 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
    10861 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga
    10921 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
    10981 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
    11041 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
    11101 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
    11161 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
    11221 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
    11281 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
    11341 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
    11401 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
    11461 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
    11521 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
    11581 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
    11641 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
    11701 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
    11761 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
    11821 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
    11881 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
    11941 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
    12001 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat
    12061 aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta
    12121 taattattga tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct
    12181 tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact
    12241 gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa
    12301 taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc
    12361 agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt
    12421 gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg
    12481 gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac
    12541 caaggagtgt acaatggact attaggagtg ggactacttt atggagctta tctttcttcc
    12601 gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc
    12661 agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg
    12721 ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga
    12781 aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc
    12841 atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt
    12901 caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg
    12961 tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt
    13021 tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa
    13081 tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa
    13141 tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt
    13201 tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag
    13261 gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag
    13321 gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag
    13381 cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg
    13441 cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt
    13501 tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt
    13561 tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca
    13621 ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa
    13681 ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc
    13741 ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat
    13801 ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc
    13861 ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg
    13921 tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt
    13981 ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta
    14041 ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta
    14101 tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc
    14161 tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac
    14221 tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg
    14281 taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc
    14341 aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc
    14401 acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt
    14461 tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt
    14521 aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag
    14581 tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg
    14641 atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc
    14701 tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc
    14761 tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac
    14821 cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt
    14881 ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg
    14941 atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga
    15001 agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat
    15061 ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc
    15121 agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc
    15181 ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca
    15241 atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt
    15301 tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag
    15361 tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca
    15421 tatgctgtca cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt
    15481 acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg
    15541 ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag
    15601 gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg
    15661 ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt
    15721 cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg
    15781 cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga
    15841 cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag
    15901 tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc
    15961 attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac
    16021 cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa
    16081 tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac
    16141 aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc
    16201 ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg
    16261 tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg
    16321 tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa
    16381 agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta
    16441 tccagtagct caagaagctt aattgaatct tatgattaaa gaggttgtga caagactttt
    16501 gtcacagcct tttttgttta taaaaataaa agttgtagtg gaggctacga agtttacttt
    16561 gtctttttac acttaatatt tttttgccaa tttggaatca ggatgttttg ataactatcg
    16621 ttttcaattg at
//
LOCUS       DAZBWR010000060        16122 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000060 DAZBWR010000000
VERSION     DAZBWR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.062"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHGT5_001710"
     CDS             226..894
                     /locus_tag="ACHGT5_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN7217694.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACHGT5_001711"
     CDS             complement(1094..1414)
                     /locus_tag="ACHGT5_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217695.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACHGT5_001712"
     CDS             complement(1451..2833)
                     /locus_tag="ACHGT5_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN7217696.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHGT5_001713"
     CDS             complement(2989..4176)
                     /locus_tag="ACHGT5_001713"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN7217697.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHGT5_001714"
     CDS             complement(4183..5214)
                     /locus_tag="ACHGT5_001714"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN7217698.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACHGT5_001715"
     CDS             complement(5260..6684)
                     /locus_tag="ACHGT5_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN7217699.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACHGT5_001716"
     CDS             6859..7764
                     /locus_tag="ACHGT5_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN7217700.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHGT5_001717"
     CDS             7855..8532
                     /locus_tag="ACHGT5_001717"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN7217701.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHGT5_001718"
     CDS             8529..8699
                     /locus_tag="ACHGT5_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217702.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHGT5_001719"
     CDS             8813..9709
                     /locus_tag="ACHGT5_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN7217703.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHGT5_001720"
     CDS             complement(9764..11134)
                     /locus_tag="ACHGT5_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN7217704.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHGT5_001721"
     CDS             11372..12118
                     /locus_tag="ACHGT5_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN7217705.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHGT5_001722"
     CDS             12115..12954
                     /locus_tag="ACHGT5_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN7217706.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACHGT5_001723"
     CDS             12955..13905
                     /locus_tag="ACHGT5_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN7217707.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACHGT5_001724"
     CDS             14046..14798
                     /locus_tag="ACHGT5_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217708.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACHGT5_001725"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACHGT5_001725"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN7217709.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5230 a         3005 c         3240 g         4647 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga at
//
LOCUS       DAZBWR010000061        16042 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000061 DAZBWR010000000
VERSION     DAZBWR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.063"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHGT5_001726"
     CDS             <1..1087
                     /locus_tag="ACHGT5_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7217710.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..2436)
                     /locus_tag="ACHGT5_001727"
     CDS             complement(1294..2436)
                     /locus_tag="ACHGT5_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7217711.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2441..2626)
                     /locus_tag="ACHGT5_001728"
     CDS             complement(2441..2626)
                     /locus_tag="ACHGT5_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN7217712.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2980..3225)
                     /locus_tag="ACHGT5_001729"
     CDS             complement(2980..3225)
                     /locus_tag="ACHGT5_001729"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217713.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3219..3620)
                     /locus_tag="ACHGT5_001730"
     CDS             complement(3219..3620)
                     /locus_tag="ACHGT5_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN7217714.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3807..5615)
                     /gene="uidA"
                     /locus_tag="ACHGT5_001731"
     CDS             complement(3807..5615)
                     /gene="uidA"
                     /locus_tag="ACHGT5_001731"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN7217715.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5638..7404)
                     /locus_tag="ACHGT5_001732"
     CDS             complement(5638..7404)
                     /locus_tag="ACHGT5_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFN7217716.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7417..8211)
                     /locus_tag="ACHGT5_001733"
     CDS             complement(7417..8211)
                     /locus_tag="ACHGT5_001733"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7217717.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8221..9597)
                     /locus_tag="ACHGT5_001734"
     CDS             complement(8221..9597)
                     /locus_tag="ACHGT5_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7217718.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9717..10364)
                     /locus_tag="ACHGT5_001735"
     CDS             complement(9717..10364)
                     /locus_tag="ACHGT5_001735"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFN7217719.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10448..11875)
                     /gene="uxaC"
                     /locus_tag="ACHGT5_001736"
     CDS             complement(10448..11875)
                     /gene="uxaC"
                     /locus_tag="ACHGT5_001736"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN7217720.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11949..13028)
                     /gene="uxuA"
                     /locus_tag="ACHGT5_001737"
     CDS             complement(11949..13028)
                     /gene="uxuA"
                     /locus_tag="ACHGT5_001737"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN7217721.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13031..14662)
                     /locus_tag="ACHGT5_001738"
     CDS             complement(13031..14662)
                     /locus_tag="ACHGT5_001738"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN7217722.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14966..15637
                     /locus_tag="ACHGT5_001739"
     CDS             14966..15637
                     /locus_tag="ACHGT5_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7217723.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15797..15988)
                     /locus_tag="ACHGT5_001740"
                     /pseudo
     CDS             complement(15797..15988)
                     /locus_tag="ACHGT5_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5187 a         3117 c         2153 g         5585 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa
     1441 ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc
     1501 tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa
     1561 aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc
     1621 aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa
     1681 ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat
     1741 taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa
     1801 aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt
     1861 atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat
     1921 tttacctata gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa
     1981 aactactctc aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat
     2041 gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg
     2101 tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc
     2161 tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg
     2221 ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg
     2281 ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac
     2341 ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct
     2401 ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat
     2461 ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa
     2521 agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta gcatagtact
     2581 gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata gcaccttcct
     2641 tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg aatttcacct
     2701 ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta aatcatcgct
     2761 ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac agcaagaaat
     2821 catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat ctatttaatt
     2881 ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac tattagaaga
     2941 ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt taaattttaa
     3001 gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta cataggtaac
     3061 gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata
     3121 atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata agataatgga
     3181 tagtggaact aataggtact gccgcttttt agatttatct accataggtc ataccccatt
     3241 ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga
     3301 attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt
     3361 cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc
     3421 attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca
     3481 tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac
     3541 ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg
     3601 tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca
     3661 atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc
     3721 tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt
     3781 ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac
     3841 catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct
     3901 ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac
     3961 acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa
     4021 gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta
     4081 tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct
     4141 agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca
     4201 taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt
     4261 gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat
     4321 tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca
     4381 atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc
     4441 gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca
     4501 tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact
     4561 acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt
     4621 ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca
     4681 ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa
     4741 ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct
     4801 tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat actatataaa
     4861 taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct
     4921 tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc
     4981 cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata
     5041 gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct
     5101 gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc attttcctgt
     5161 actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt gtttacagca
     5221 actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc aaatggtgta
     5281 aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc ttcatgtgtt
     5341 gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag agtcacttct
     5401 ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta
     5461 taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc taaagcaagt
     5521 tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct taagtcaatt
     5581 acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat ctaaatatta
     5641 aataaatgta tcctttaaac cacaataagc atctattgga cttactccct taaattcaga
     5701 aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat cataagtatt
     5761 aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta cagatacaaa
     5821 aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac ttgctggcca
     5881 gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca agtcatattt
     5941 tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat ttttaatttt
     6001 ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt caacttcaaa
     6061 tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat ttccagcaac
     6121 tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc ttttatattt
     6181 ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg cttggtcagc
     6241 cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag cttcttgctt
     6301 aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt ttaaccctaa
     6361 aattcttttt aaagcatcta ataaccgatc atcagtgata attcctttac gatagccttc
     6421 catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat cacatccagc
     6481 tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc ctaacatatg
     6541 actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta agtcagtgat
     6601 taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag gattaaaatg
     6661 ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc cagcttctat
     6721 taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta aactattaat
     6781 actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat gtttagctgc
     6841 aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt ctaaaacttt
     6901 atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat ttctattaat
     6961 atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa tcccagaaac
     7021 tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta cttctacacc
     7081 aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag cagcaattaa
     7141 tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct catcagcagt
     7201 tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac tcaagtaatt
     7261 tttatcacga ctagatccca tattaacaaa caattgacct attttttctt ccaaagtcat
     7321 agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat atggtttttt
     7381 tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa gatttttcca
     7441 aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac atttaaataa
     7501 aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa taggataaaa
     7561 gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa tcattaatat
     7621 cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt tttttccttt
     7681 atcttgacaa cacagttcat caactaatga taataattca tgtctttccc attcttcttg
     7741 aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt tcagagcttt
     7801 tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac taatctgttg
     7861 attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag aatattctat
     7921 ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt gctttaatcg
     7981 attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa atctgtttat
     8041 tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt tttctctatg
     8101 atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt tataagtcat
     8161 cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca tatttctctc
     8221 ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt tagataatgg
     8281 ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa ttaatgttga
     8341 agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag agtcatagcc
     8401 tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta tttttctagc
     8461 aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta cttcttgatc
     8521 atcaataaca tcagtaatat ttgcccaaat aacagtatta aacacaccca aacccatata
     8581 gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca aaaataagat
     8641 caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt ttccatattt
     8701 tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag gattgataaa
     8761 attcattaaa actattccct gtgcatcatt atagtaatca ggaaatacat aattattcat
     8821 actcataatt aataattgag caaggagtaa gaaaatagcc gcaccaatta tagaaagtaa
     8881 tgccctattt ttaactaacc cttttaaaga ttttaacaat gaaactttgt attgagattg
     8941 gtcaattctt actcgttcag tagttagttt ataacatatt aaataacaaa taagtgcaca
     9001 tatcgaaaat attcccgcaa caattgtaaa agttgatcca ccacgaacta tttgagcgcc
     9061 tgaatcatct cttgtataaa taaataatgg tgttaataca ccaatgacca aacttgctaa
     9121 agtagctcca acatttcgga atgtcgatag cattgttctt tcttctgtaa tagaagtaat
     9181 agctgatgcc attgatccat aaggaatatt tatagatgtg taaaaaatac tcccccataa
     9241 tatatacgta acatacatgt atactacctt aacgctcata gaagctgaaa tcatagaagt
     9301 ttgatacatt aaaaaacttg ctaaagcgac tggaccactc attcttaaaa tccacggttt
     9361 aaacttaccc tgcttagttg ctttcgatct atcaacaata cgccccatag ttacatcagt
     9421 aactgcatcg ataaatcttg ctactaaaaa taatgttcca acggcagctc cgcttatccc
     9481 caatactttt gtataaaaga tcattaaaaa agaactagca aaaatgaaag taaaatcgtt
     9541 tccaaaatct ccaaacatat atcctatttt gtctcttata ccaaattttc tttccataca
     9601 attctctccc ttctgttgtt aagcgttttc attctgatga aagtacgtac tatactctga
     9661 agcgtaaaga gtcagtattt attttttaca tttcagagta ttatttgttt tttctcctaa
     9721 taattcttta tctcataaaa cttatctata tatttactag ctactcgact tacttcttca
     9781 aaatcacctt tttcaacaga agccaataaa ttcccaccta caccaactgc aacaactcct
     9841 acttcaaacc aatcttctaa gttctctata cttactccgc cagtaggcat tatatttacg
     9901 tgtggtaatg gagcttgata ggcttttaca atggatggac caaaagcaga acctgggaat
     9961 agtttaatga tatctacgcc agcagtaagt gcctccttta tctcagtaat tgtcatacaa
    10021 cctggtaaat aaggaatttg atataaatta caaatctcag ctgtttgtcg atcaaaagat
    10081 ggactaacga tatattctgc cccagccata attgcgatac gagcagtaaa cgcatctaaa
    10141 accgtaccgg ctccgattat tacatctttt tgatcagcat attttttagc aatttctttg
    10201 attatttctt cagcattagg aactgtaaaa gttagttcaa tccctttaat tccacccgca
    10261 ataattgaat ttattgcttt aatggcctca tccttatttt tcccacgtac aaccgcaatg
    10321 actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcctt
    10381 tatggataca tactttgtct agctgtacta acttacaaag aataaattta agcttttatt
    10441 tcaagtatta ttttctactt ccgatttcaa aaaaattaaa ataattatac gcattacgat
    10501 aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt cggcctgctt
    10561 taacccactc gcttagtaat tgacataata ctcgacgaaa atactcatgg cgaggatagc
    10621 ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgtg
    10681 ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc
    10741 ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa
    10801 gccaatcagt tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag
    10861 cggtatcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac
    10921 caccgacatc tactccaatt tgttccttta atatacttga attatttctt aaagcattta
    10981 aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt aattgaatac
    11041 ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct aaagctttta
    11101 ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta ttcaaaccgt
    11161 gatctgctaa tcgacagcca atttgatgaa aatgatccac tcgttggctc aatgcttgct
    11221 tcaacccatt aaagtctttg attggtatag ccgctgtttg actaagtttt aaaatatagt
    11281 tcgaaaagcc tgatgatttg atactccaga ccttatctgg acggaaagta ggtaatacct
    11341 tgaaagaatt atcctgttta gaaagctcag tatgatacca caagtcatct gtaggatcat
    11401 ccgttgtaca gatggctttc acatccatca tctccaacaa ttctttcggg cgataggaat
    11461 ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg
    11521 tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa ataggattac
    11581 ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca tcacctggac
    11641 cactgatcaa ctcttcttct ataccattag ctcgcatcaa tcgccattta taatgatctc
    11701 cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta ttttctagca
    11761 aatatgctgg gtctaaatga caatgatagt cgataattgg ttggtgtttt gcaaatttat
    11821 gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa aacatattta
    11881 aacactcctt tccttccgtt agtttttaga agagaaaatt tttcctctaa ataaactccc
    11941 ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt taaataaact
    12001 gcacctagag ctcggtcata taatccataa ccaggtctac cggtttctcc ccaaatcatc
    12061 cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt ttttaatatc
    12121 ttatacatat ctagagaacc atattctgat ttatgcggcg cttcgtaaaa atcgagtcca
    12181 ttgtcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa
    12241 aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact tccagtacat
    12301 aatgtaatac cattagaagg agaatccact acattacaca accaatctaa atcgtcacga
    12361 tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg gtgaactgcc
    12421 attttaatcc caacttcttc acatgccggg ataattgctt tgagaaaata tgcaaagttc
    12481 tctcgtaatt tctcttcatc aacttcttta taggcttcta ataattgctt aatttgtgat
    12541 aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc tacttctcta
    12601 ataatttcct ccggatcagt agggatattt tttgaaatat aggctaacgt agaagaccca
    12661 tcatctaaac gataatccaa atccgatttt acccaatcaa atactggcat aaaattataa
    12721 cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata attctcaatg
    12781 tattgatctc gtgttggtaa accaattttg atatcatcat gaacattaac actctcaata
    12841 actttcaagg taagtccatt ggcttcgact tcatctttta aagatttaat tcgttctctc
    12901 ggccaaacct ctccggctgg tatatccatt aatgttccaa caattccttt cattgctggg
    12961 atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca tctaaaagtc
    13021 atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt ttacgaactg
    13081 cttgaggacc tactagcatt tcgcaaaaga ttttttcagt tttctttcct accccaactt
    13141 gatataaatc taaaccaaag atttctttat tttctaaaat ttcctgtacc gcacaatgaa
    13201 tctttgaagg atcttgttca cctaatttca atactgataa tttatcctgc aactctacca
    13261 ataacggatc aggacttggc acaaaatttt ccccttgatc atcgactgcc agtaaataac
    13321 gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt ccgtcttttt
    13381 cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa gtatctgttg
    13441 caattcgttg tggtgtatct ggaatatagg gattaggcaa acgtttatta attacttcat
    13501 caataaattg ttttggatca ataatgcctg gattttcaac tactggtaaa ccttctaaat
    13561 aacctattcc tttgattaaa ccaaccaaat catcatcctg catctctgct gaaattgatt
    13621 gatatcctag cagacaacct gttactgcca aagcagtatg aagtggattc aagcttgtcg
    13681 taaccttcat gatgtctgct ttatcaaccg tctctctatc tgtcataatc acaccagcct
    13741 tttccaattt aggccggcca tttggaaaac tatcttcaat aactaaataa tgaactacct
    13801 cagtattagt gaaagcagca atattggtat gtttttttgt gtgaatgata tctaaatcct
    13861 cgagccccat catcaataga tcttccttta cattctctga aggatttggc gtaattcgat
    13921 caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca ataaattttt
    13981 cagaaacata tccttctttt tgccaagctt ctgcaatcgt aaaaatacta tcttgaaaac
    14041 gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac tctccagcat
    14101 taaatctgct atataacaag gatgttacaa tactcatcgt atgttgagga ttgctaggtc
    14161 cagatttgat atcagctttg actaccgaaa agaattgccc tttagaatct tttaaactgt
    14221 aacctttttc agtaatagta aacgttgtaa actgtaattt aggatcttca aaatatttaa
    14281 ccactctttg atagtccgct atattcttcg gattgcaata taaactatca gccgttactg
    14341 ccaaaagtct ttttttgagt tcaccatctc cactcataac aatttccaaa atgtcattgt
    14401 tatagtcatg ataagctttt tccactactt cttcatcaaa tgtttcacac acaactactc
    14461 ctgtttctaa ttcattttga tctgctaagc attgagcaat ttctgcatga aatcctcgat
    14521 aaagatttcc tccaccaaaa tgaagccata ctggatgttg ctggccttct tgcttaactt
    14581 ctttgacatc aaaacacggc agtagaatct gcatttcatt gaattcttcg atagaatttt
    14641 gccaattttt aatcgaaacc ataattaacc tcctcaaact gatatgttag ttttgataca
    14701 ttttatattt taaaacccat tgtaaccgtt gtcaatataa aaagggaata aacttgcttt
    14761 tttctaccat atcagaatga ttgtattttt tattcagaaa aataaaatac tccttctttt
    14821 tggactgatt ttatcgaaac ttattttttc tttttttcat gtattctttt atttttataa
    14881 ctagtgtatt acttcaaaat ttgatattat atggcatgaa cagaattaaa atatcagtta
    14941 aaataaaagt taaggagcat tgtgtatgac aattaaaact agtctccaaa atcaagctta
    15001 ccaatctatt cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa aagtatcaga
    15061 aaaaaaatta gaagaaacac tttctattgg tcgtacacca atacgtgaag cactgattca
    15121 actccgacaa caaggtcttg ttgatacaat tcctcaaagc ggaaattata tctcattaat
    15181 tgatttagat tcggccaaaa atgctcgatt tgtcagagaa catttggaac gacaaattat
    15241 gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa ctataatttc
    15301 agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata atgataattt
    15361 attccataaa gcttgttttg aaattgcaga gcgagaagaa gtatggcaat ggttagaaaa
    15421 taacaataca cacttagaac gatttcgatg gctacgagta aaaacggaag gattaaagtg
    15481 ggaaaaaatt atggaacaac attatcaact atttgaggca ttaacaaaac aagatccaga
    15541 agaagctaac tttctaacat cgttgcatct tcatttaatg cttaatgagc aaaatattgt
    15601 tttagctact catccaaaat atttcaaaaa tatataactt ttaactcttt ttaaattaaa
    15661 tggttgttct gatcagaaaa atatcgcttg ttttcttatt atgtaatata tttcataaac
    15721 tattctgcat aacaactatt gtttgtactt tttacattgg ataactaatt atgactgctg
    15781 ataacttttg atattaaact agtatctcca atacaaataa aaaatagata tcttgttcat
    15841 caaaattagt tcgtggatac ttaccttcat ttacatcatt cagcggtttc ccattttctt
    15901 ttattctatt atttcatttt ctaatagact tggcttcatt tcgcaatact ttttttcaat
    15961 aggcatcaaa ttggtgttgt ttagatattt agttgtagtt ttgtgacatg ataagtatgc
    16021 tcctttttct cttttccact aa
//
LOCUS       DAZBWR010000062        15801 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000062 DAZBWR010000000
VERSION     DAZBWR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.064"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..781
                     /locus_tag="ACHGT5_001741"
     CDS             29..781
                     /locus_tag="ACHGT5_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN7217724.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="ACHGT5_001742"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="ACHGT5_001742"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN7217725.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="ACHGT5_001743"
     CDS             1710..3641
                     /locus_tag="ACHGT5_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN7217726.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3752..5311)
                     /locus_tag="ACHGT5_001744"
     CDS             complement(3752..5311)
                     /locus_tag="ACHGT5_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN7217727.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHGT5_001745"
     CDS             5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHGT5_001745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN7217728.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7717..9753
                     /gene="ligA"
                     /locus_tag="ACHGT5_001746"
     CDS             7717..9753
                     /gene="ligA"
                     /locus_tag="ACHGT5_001746"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN7217729.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9859..10164
                     /gene="gatC"
                     /locus_tag="ACHGT5_001747"
     CDS             9859..10164
                     /gene="gatC"
                     /locus_tag="ACHGT5_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN7217730.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10164..11636
                     /gene="gatA"
                     /locus_tag="ACHGT5_001748"
     CDS             10164..11636
                     /gene="gatA"
                     /locus_tag="ACHGT5_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN7217731.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11633..13063
                     /gene="gatB"
                     /locus_tag="ACHGT5_001749"
     CDS             11633..13063
                     /gene="gatB"
                     /locus_tag="ACHGT5_001749"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN7217732.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13073..14128
                     /locus_tag="ACHGT5_001750"
     CDS             13073..14128
                     /locus_tag="ACHGT5_001750"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN7217733.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14239..15612
                     /gene="rlmD"
                     /locus_tag="ACHGT5_001751"
     CDS             14239..15612
                     /gene="rlmD"
                     /locus_tag="ACHGT5_001751"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN7217734.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15615..15743
                     /locus_tag="ACHGT5_001752"
     CDS             15615..15743
                     /locus_tag="ACHGT5_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN7217735.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5373 a         2897 c         3469 g         4062 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc tttttaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga aactgaaatt
     1081 aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga aatcattccg
     1381 tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa agaagaggct
     3661 ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa
     3721 tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag
     3781 aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa
     3841 ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc
     3901 tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt
     3961 ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg
     4021 cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga
     4081 gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa
     4141 gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg
     4201 tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga
     4261 aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa
     4321 ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg aatcgaagaa
     4381 caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt
     4441 gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag
     4501 gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca
     4561 gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg
     4621 aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc
     4681 cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa
     4741 caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag
     4801 cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg
     4861 aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag
     4921 aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact
     4981 ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt
     5041 gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg
     5101 ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt
     5161 catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga
     5221 cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat
     5281 ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc
     5341 gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag
     5401 ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt
     5461 tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg
     5521 ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag
     5581 tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc
     5641 tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc
     5701 tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt
     5761 taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg
     5821 aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg
     5881 atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg
     5941 attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt
     6001 accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga
     6061 ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa ttccattata
     6121 ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg
     6181 caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc
     6241 gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa
     6301 ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca
     6361 ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca
     6421 agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagatcgga
     6481 tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga
     6541 cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa
     6601 tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga
     6661 atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac
     6721 gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc
     6781 cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc
     6841 aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca tacttgacag
     6901 tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc
     6961 aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac
     7021 aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa
     7081 aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat ttagaagaag
     7141 atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa ttcccagttg
     7201 tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa
     7261 gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt
     7321 tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt
     7381 ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc
     7441 ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag
     7501 cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata
     7561 aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg
     7621 atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg
     7681 cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac
     7741 gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac
     7801 tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta
     7861 gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta
     7921 ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg
     7981 aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg
     8041 ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt
     8101 tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac
     8161 atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac
     8221 atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac
     8281 agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg
     8341 cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta
     8401 gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa
     8461 attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca
     8521 tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc
     8581 atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa agcaccgaga
     8641 tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa
     8701 tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta
     8761 gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat
     8821 atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg
     8881 caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt
     8941 ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat
     9001 cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg
     9061 aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca
     9121 gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa
     9181 gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag
     9241 cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca
     9301 gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt
     9361 gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc
     9421 catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga
     9481 aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt
     9541 aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa
     9601 gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc
     9661 aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat
     9721 gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca
     9781 ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt
     9841 ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta
     9901 gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc
     9961 atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt tacgtcgaat
    10021 gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat
    10081 gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc
    10141 gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc
    10201 atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca acatttgaac
    10261 gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa gaaaaagcat
    10321 tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta
    10381 ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta
    10441 agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag
    10501 cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag
    10561 aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct
    10621 cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg
    10681 atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta
    10741 cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg
    10801 gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg
    10861 acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag
    10921 gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc
    10981 ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg
    11041 tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct
    11101 catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt tatcgctcag
    11161 aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag
    11221 aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat tatgacgctt
    11281 acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg
    11341 cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga
    11401 acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg
    11461 ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc
    11521 agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac
    11581 aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt
    11641 tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc
    11701 tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg
    11761 ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc
    11821 tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta
    11881 cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg
    11941 atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt
    12001 agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa
    12061 ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga
    12121 agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt
    12181 gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca
    12241 agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa
    12301 aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca
    12361 acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga
    12421 agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga
    12481 cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg
    12541 ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga
    12601 aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa
    12661 ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac
    12721 aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag
    12781 ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga
    12841 agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat
    12901 taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg
    12961 tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg
    13021 tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa
    13081 agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga
    13141 tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga
    13201 accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt
    13261 ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca
    13321 ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa
    13381 aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa
    13441 aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt
    13501 gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta
    13561 tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta
    13621 tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt
    13681 tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat
    13741 cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg
    13801 cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga
    13861 aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga
    13921 agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca
    13981 gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga
    14041 attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc
    14101 agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag
    14161 tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata
    14221 ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca
    14281 gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca
    14341 ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc
    14401 aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct
    14461 gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa
    14521 caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg
    14581 ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa
    14641 gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat
    14701 agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca
    14761 atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa
    14821 aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg
    14881 gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt
    14941 catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat
    15001 gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta
    15061 ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca
    15121 gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc
    15181 gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg
    15241 tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat
    15301 aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa
    15361 gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat
    15421 cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat
    15481 ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa
    15541 gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc ttcattcgtt
    15601 aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag
    15661 gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac atgagaaaag
    15721 gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt taacttttgt
    15781 tattgtttct tagtcttttt a
//
LOCUS       DAZBWR010000063        15255 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000063 DAZBWR010000000
VERSION     DAZBWR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.065"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACHGT5_001753"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACHGT5_001753"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN7217736.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHGT5_001754"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHGT5_001754"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN7217737.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACHGT5_001755"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACHGT5_001755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN7217738.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACHGT5_001756"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACHGT5_001756"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN7217739.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACHGT5_001757"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACHGT5_001757"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN7217740.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHGT5_001758"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHGT5_001758"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN7217741.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACHGT5_001759"
     CDS             6726..7466
                     /locus_tag="ACHGT5_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN7217742.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACHGT5_001760"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACHGT5_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN7217743.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHGT5_001761"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHGT5_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN7217744.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACHGT5_001762"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACHGT5_001762"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN7217745.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACHGT5_001763"
     CDS             11260..11898
                     /locus_tag="ACHGT5_001763"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN7217746.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACHGT5_001764"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACHGT5_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN7217747.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACHGT5_001765"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACHGT5_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN7217748.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="ACHGT5_001766"
     CDS             13675..15249
                     /locus_tag="ACHGT5_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN7217749.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5114 a         2864 c         3319 g         3958 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatga
//
LOCUS       DAZBWR010000064        15141 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000064 DAZBWR010000000
VERSION     DAZBWR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.066"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(498..1064)
                     /locus_tag="ACHGT5_001767"
     CDS             complement(498..1064)
                     /locus_tag="ACHGT5_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7217750.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1064..2020)
                     /locus_tag="ACHGT5_001768"
     CDS             complement(1064..2020)
                     /locus_tag="ACHGT5_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN7217751.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2020..2676)
                     /locus_tag="ACHGT5_001769"
     CDS             complement(2020..2676)
                     /locus_tag="ACHGT5_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN7217752.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3046..5064
                     /locus_tag="ACHGT5_001770"
     CDS             3046..5064
                     /locus_tag="ACHGT5_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN7217753.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5134..5340
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5491..7905
                     /gene="leuS"
                     /locus_tag="ACHGT5_001771"
     CDS             5491..7905
                     /gene="leuS"
                     /locus_tag="ACHGT5_001771"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN7217754.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8253..9806
                     /locus_tag="ACHGT5_001772"
     CDS             8253..9806
                     /locus_tag="ACHGT5_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN7217755.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10187..11971)
                     /locus_tag="ACHGT5_001773"
     CDS             complement(10187..11971)
                     /locus_tag="ACHGT5_001773"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217756.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11986..13707)
                     /locus_tag="ACHGT5_001774"
     CDS             complement(11986..13707)
                     /locus_tag="ACHGT5_001774"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217757.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13807..14568
                     /locus_tag="ACHGT5_001775"
     CDS             13807..14568
                     /locus_tag="ACHGT5_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN7217758.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4937 a         2771 c         2980 g         4453 t
ORIGIN      
        1 ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa ttcttccatc
       61 ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag cgatatttga
      121 ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat atgaatgaca
      181 tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc attcttttat
      241 atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa taaagaggtt
      301 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
      361 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
      421 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
      481 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
      541 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
      601 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
      661 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
      721 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
      781 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
      841 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
      901 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
      961 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
     1021 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
     1081 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
     1141 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
     1201 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
     1261 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
     1321 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
     1381 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
     1441 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
     1501 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
     1561 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
     1621 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
     1681 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
     1741 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
     1801 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
     1861 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
     1921 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
     1981 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
     2041 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
     2101 aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
     2161 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
     2221 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
     2281 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
     2341 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
     2401 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
     2461 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
     2521 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
     2581 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
     2641 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
     2701 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
     2761 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
     2821 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
     2881 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
     2941 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
     3001 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
     3061 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
     3121 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
     3181 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
     3241 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
     3301 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
     3361 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
     3421 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
     3481 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
     3541 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
     3601 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
     3661 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
     3721 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
     3781 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
     3841 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
     3901 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
     3961 tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg tacatcaaac
     4021 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
     4081 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
     4141 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
     4201 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
     4261 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
     4321 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
     4381 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
     4441 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
     4501 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
     4561 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
     4621 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
     4681 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
     4741 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
     4801 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
     4861 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
     4921 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
     4981 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
     5041 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
     5101 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
     5161 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
     5221 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
     5281 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
     5341 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
     5401 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
     5461 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
     5521 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
     5581 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
     5641 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
     5701 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
     5761 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
     5821 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
     5881 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
     5941 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
     6001 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
     6061 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
     6121 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
     6181 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
     6241 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
     6301 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
     6361 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
     6421 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
     6481 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
     6541 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
     6601 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
     6661 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
     6721 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
     6781 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
     6841 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
     6901 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
     6961 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
     7021 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
     7081 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
     7141 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
     7201 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
     7261 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
     7321 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
     7381 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
     7441 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
     7501 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
     7561 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
     7621 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
     7681 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
     7741 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
     7801 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
     7861 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
     7921 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
     7981 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
     8041 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
     8101 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
     8161 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
     8221 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
     8281 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
     8341 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
     8401 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
     8461 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
     8521 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
     8581 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
     8641 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
     8701 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
     8761 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
     8821 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
     8881 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
     8941 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
     9001 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
     9061 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
     9121 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
     9181 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
     9241 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
     9301 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
     9361 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
     9421 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
     9481 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
     9541 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
     9601 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
     9661 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
     9721 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
     9781 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
     9841 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
     9901 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
     9961 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    10021 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    10081 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    10141 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    10201 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    10261 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    10321 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    10381 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    10441 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    10501 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    10561 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    10621 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    10681 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    10741 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    10801 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    10861 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    10921 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    10981 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    11041 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    11101 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    11161 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    11221 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    11281 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    11341 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    11401 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    11461 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    11521 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    11581 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    11641 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    11701 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    11761 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    11821 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    11881 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    11941 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    12001 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    12061 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    12121 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    12181 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    12241 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    12301 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    12361 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    12421 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    12481 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    12541 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    12601 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    12661 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    12721 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    12781 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    12841 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    12901 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    12961 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    13021 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    13081 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    13141 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    13201 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    13261 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    13321 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    13381 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    13441 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    13501 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    13561 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    13621 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    13681 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    13741 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    13801 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    13861 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    13921 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    13981 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    14041 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    14101 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    14161 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    14221 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    14281 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    14341 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    14401 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    14461 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    14521 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    14581 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    14641 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    14701 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    14761 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    14821 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    14881 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    14941 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    15001 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    15061 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    15121 aaggttattg ttatttttat a
//
LOCUS       DAZBWR010000065        15138 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000065 DAZBWR010000000
VERSION     DAZBWR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.067"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHGT5_001776"
     CDS             5..2377
                     /locus_tag="ACHGT5_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN7217759.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHGT5_001777"
     CDS             2438..3898
                     /locus_tag="ACHGT5_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN7217760.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHGT5_001778"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHGT5_001778"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN7217761.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHGT5_001779"
     CDS             6129..6278
                     /locus_tag="ACHGT5_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217762.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHGT5_001780"
     CDS             6353..6946
                     /locus_tag="ACHGT5_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN7217763.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHGT5_001781"
     CDS             6959..7858
                     /locus_tag="ACHGT5_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN7217764.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHGT5_001782"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHGT5_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN7217765.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHGT5_001783"
     CDS             8724..8984
                     /locus_tag="ACHGT5_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217766.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHGT5_001784"
     CDS             9429..9635
                     /locus_tag="ACHGT5_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN7217767.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHGT5_001785"
     CDS             9656..9886
                     /locus_tag="ACHGT5_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217768.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHGT5_001786"
     CDS             9902..10339
                     /locus_tag="ACHGT5_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217769.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHGT5_001787"
     CDS             10332..11039
                     /locus_tag="ACHGT5_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217770.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHGT5_001788"
     CDS             11203..11403
                     /locus_tag="ACHGT5_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217771.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHGT5_001789"
     CDS             11419..11598
                     /locus_tag="ACHGT5_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217772.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHGT5_001790"
     CDS             11923..12186
                     /locus_tag="ACHGT5_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFN7217773.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHGT5_001791"
     CDS             12180..12530
                     /locus_tag="ACHGT5_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN7217774.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHGT5_001792"
     CDS             complement(12554..13168)
                     /locus_tag="ACHGT5_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7217775.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACHGT5_001793"
     CDS             13482..13904
                     /locus_tag="ACHGT5_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217776.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACHGT5_001794"
     CDS             13914..14117
                     /locus_tag="ACHGT5_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217777.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACHGT5_001795"
     CDS             14328..14912
                     /locus_tag="ACHGT5_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7217778.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3061 g         4056 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
    15001 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    15061 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    15121 tttcaaatta attagtaa
//
LOCUS       DAZBWR010000066        15042 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000066 DAZBWR010000000
VERSION     DAZBWR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.068"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(286..358)
                     /locus_tag="ACHGT5_001796"
     tRNA            complement(286..358)
                     /locus_tag="ACHGT5_001796"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Lys,seq:ctt)
     gene            739..1308
                     /locus_tag="ACHGT5_001797"
     CDS             739..1308
                     /locus_tag="ACHGT5_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN7217779.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1295..2155
                     /locus_tag="ACHGT5_001798"
     CDS             1295..2155
                     /locus_tag="ACHGT5_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN7217780.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2345..3049
                     /gene="yycF"
                     /locus_tag="ACHGT5_001799"
     CDS             2345..3049
                     /gene="yycF"
                     /locus_tag="ACHGT5_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN7217781.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3054..4889
                     /gene="walK"
                     /locus_tag="ACHGT5_001800"
     CDS             3054..4889
                     /gene="walK"
                     /locus_tag="ACHGT5_001800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN7217782.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4886..6202
                     /gene="yycH"
                     /locus_tag="ACHGT5_001801"
     CDS             4886..6202
                     /gene="yycH"
                     /locus_tag="ACHGT5_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN7217783.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6203..7072
                     /locus_tag="ACHGT5_001802"
     CDS             6203..7072
                     /locus_tag="ACHGT5_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN7217784.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7127..7936
                     /locus_tag="ACHGT5_001803"
     CDS             7127..7936
                     /locus_tag="ACHGT5_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN7217785.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8039..8668)
                     /locus_tag="ACHGT5_001804"
     CDS             complement(8039..8668)
                     /locus_tag="ACHGT5_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN7217786.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8838..10130
                     /locus_tag="ACHGT5_001805"
     CDS             8838..10130
                     /locus_tag="ACHGT5_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7217787.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10206..10868)
                     /locus_tag="ACHGT5_001806"
     CDS             complement(10206..10868)
                     /locus_tag="ACHGT5_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN7217788.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11093..11377
                     /gene="groES"
                     /locus_tag="ACHGT5_001807"
     CDS             11093..11377
                     /gene="groES"
                     /locus_tag="ACHGT5_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN7217789.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11428..13053
                     /gene="groL"
                     /locus_tag="ACHGT5_001808"
     CDS             11428..13053
                     /gene="groL"
                     /locus_tag="ACHGT5_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN7217790.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13142..14281)
                     /locus_tag="ACHGT5_001809"
     CDS             complement(13142..14281)
                     /locus_tag="ACHGT5_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7217791.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(14392..14976)
                     /locus_tag="ACHGT5_001810"
     CDS             complement(14392..14976)
                     /locus_tag="ACHGT5_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN7217792.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         4875 a         2593 c         3131 g         4443 t
ORIGIN      
        1 tattcttcac tttttaacct tttatagtac ttagatttaa tcctatatgt ttagagagtt
       61 cattggcagc atgattttgc aaagcttctc gaattattgt tatattagaa actttactgt
      121 tcataattat gcttcttttt taactcaaag tccaaagggc tacagtggac tttgagttaa
      181 aaataaaaag ccctagttat tttgaaaaat aaaaaaagaa cagcgtaaat gttttcgaaa
      241 tgctttcgca ccaacattta cctgttccgt gggtttggca cccaatgggc cgtgaggggc
      301 tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt
      361 acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag
      421 aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct
      481 cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt
      541 ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa
      601 aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca
      661 cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg
      721 aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa
      781 ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac
      841 caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca
      901 ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt
      961 aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa
     1021 gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt
     1081 cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa
     1141 ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt
     1201 gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt
     1261 ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt
     1321 agtagattca ggtacggatg ttccagaaga attgataaaa aaatacgaga tgtatatgat
     1381 cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca ttacccctga
     1441 agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc
     1501 gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac
     1561 aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga
     1621 agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc
     1681 gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct
     1741 gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca
     1801 aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc
     1861 aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa
     1921 aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt
     1981 caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact
     2041 taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt
     2101 agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa
     2161 aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa
     2221 aataataaag tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg
     2281 aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct
     2341 ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt
     2401 caatttagcc aaagaaggct atgacgttta tactgcatat gatggggaag aagccttgga
     2461 aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga
     2521 cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac
     2581 agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt
     2641 gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg
     2701 tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt
     2761 gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca
     2821 tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga
     2881 acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt
     2941 aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt
     3001 gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa
     3061 gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt
     3121 tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga
     3181 tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc
     3241 cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat
     3301 ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga
     3361 tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg
     3421 ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg
     3481 tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt
     3541 acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct
     3601 ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt
     3661 cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact
     3721 acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca
     3781 atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt
     3841 tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag
     3901 tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg
     3961 gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag
     4021 aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga
     4081 tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag
     4141 ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct
     4201 ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat
     4261 tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag
     4321 atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata
     4381 caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc
     4441 ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat
     4501 ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata
     4561 atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat
     4621 tagaaacgca taataacgtg attttaagta tcaccgatca aggattgggg attcctaaaa
     4681 aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac
     4741 aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg
     4801 ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg
     4861 aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact
     4921 cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg
     4981 aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg
     5041 aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca
     5101 agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt
     5161 tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct
     5221 gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc
     5281 atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac
     5341 tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct
     5401 cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa
     5461 agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac
     5521 gaaacattcg atcaaactga agaagtatag ttatatcctg tcacagcaac cctatacact
     5581 ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga
     5641 attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga
     5701 ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc
     5761 ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa
     5821 tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg
     5881 acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac
     5941 cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac
     6001 gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt
     6061 gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc
     6121 tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt
     6181 acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc
     6241 ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa
     6301 caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat
     6361 cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca
     6421 gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc
     6481 aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga
     6541 aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa
     6601 agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga
     6661 agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac
     6721 agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat
     6781 gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga
     6841 ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg
     6901 gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt
     6961 ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagcg tcaacgcttt
     7021 cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga
     7081 gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa
     7141 accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga
     7201 aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt
     7261 gatggctcaa attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca
     7321 tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc
     7381 caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa
     7441 acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg
     7501 cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt
     7561 cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc
     7621 cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc
     7681 gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc
     7741 acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag
     7801 caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa
     7861 agattttggc gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga
     7921 gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa
     7981 tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt
     8041 atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta
     8101 atgcaatcgc gtaatctgcc atgctgatcg tactgtcgct agcagcgttt ctttgtaaat
     8161 aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg
     8221 gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca
     8281 tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg
     8341 tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg
     8401 ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt
     8461 tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact
     8521 caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga
     8581 ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc
     8641 cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt
     8701 aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct
     8761 ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa
     8821 gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat
     8881 ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa
     8941 taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc
     9001 agtggataga caaaacagga ttttctatgt attttacaca agtataccag gctacatttg
     9061 atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca
     9121 tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac
     9181 taaatactca gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa
     9241 ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc
     9301 aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg
     9361 ttatagtcac ttttacgctt atcggttcag cggtttttca acatttgttc agaatgactg
     9421 ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt
     9481 ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat
     9541 ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta gcaaatgcag
     9601 aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc
     9661 aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt
     9721 ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac
     9781 cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga
     9841 tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt
     9901 tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga
     9961 tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt
    10021 caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa
    10081 aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa
    10141 tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt
    10201 catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt
    10261 cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag
    10321 aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc
    10381 aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata
    10441 ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa
    10501 gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg
    10561 ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt
    10621 ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc
    10681 gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac
    10741 atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg
    10801 atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt
    10861 aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat
    10921 tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg
    10981 atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc
    11041 gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa
    11101 accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg
    11161 aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg
    11221 tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca
    11281 agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat
    11341 cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc
    11401 aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca
    11461 cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga
    11521 cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat
    11581 ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa
    11641 cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca
    11701 actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat
    11761 cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat
    11821 aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg
    11881 ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt
    11941 gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg
    12001 cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct
    12061 gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt
    12121 ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat
    12181 gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta
    12241 gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct
    12301 attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa agatgcaaca
    12361 atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta
    12421 gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt
    12481 gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca
    12541 ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa
    12601 ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca
    12661 ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga
    12721 gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc
    12781 gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta
    12841 ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta
    12901 gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta
    12961 ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca
    13021 atggatccat caatgatggg cggtatgatg taggaattaa aagagaaccc ttatattaca
    13081 gggttctctt ttcttttttt gttttatttt aagtaaaagg ggcaaaaaag gggcagaaat
    13141 atcaaagcct atttagttta tcaattactt gttgttttgc ttttttggtt acgtgattgt
    13201 aaatagacag agttgtattt gcatcagaat gtcctacacg ctccataatg gctttaagag
    13261 gtacgcctaa ttcagataat agcgaaacat ggctatgtct aaatatatgc gatgatagat
    13321 ttttttctaa ttccaactct tcttctactt tatggagtac agcgttaaat gagtgcagcg
    13381 taagcggagt gccatttgta gatataaata tatattgatc gggattggtg ggtctacctg
    13441 caagaatatt gtcagctatc acactttcaa tcaattcttt tgcacgattg gataattgta
    13501 cttcacgttg tgagtaagta ttttttggag ttgtttttat ggcgttatcc atttttacag
    13561 atgtgtaatc taaggtccca ttaatggaaa ttttaccctc tccatagtcc ttcatttgca
    13621 aagccagcaa ttccccatat ctcaaaccag tcaaatatag aaattcagct attatgccgt
    13681 gtagttttct gcgaggattg gaatataact gtttcagtat ttgatcaatt tcttctttct
    13741 ccagatattt tttatccata gaaagctttc ttttttcttc ttccactttt ttaggatgga
    13801 ttttaactgc tagagcaggg tttctttgaa tgtattttct atcaattgca tagtttagca
    13861 taacggatag agttgttttt gtttgctttg tgtagttcag tgagagatca ccaaaagtgt
    13921 acatatcttc aatcatctta ttaatgagtg tctcatcaat gtttctaacg attgtatcat
    13981 cgcttatgtg cttagaaaca tgtttcatca tcattggaac cttcaaatag ctagtacgtt
    14041 taacatgctg cttataatat tcataccatt ctttatacag ctcaccaaaa gtgatatctg
    14101 atttattgta attttcgagt gcttctttaa tttttttatc tagaattttc tgagcttttt
    14161 tccacgcttg tggtgaatta cttgtaagtg ttgtagattt ttttcgtgtt ttttctgtat
    14221 aaggatctac atatctttca ataaacttga attggccatc tttggtttgt tcaacccaca
    14281 cttttaacat ctcctatcat ttgctataat aggcataaca aatagaccta tataggtttg
    14341 ttttctaaaa gcacgctctt actctggacg gtggggcgtg ttttttattt attatacagt
    14401 tgctttaaaa atagctgctt tttcaaatga tactaaagga gagaagtgga tttctatttc
    14461 tcccttttgg tttaacgtga aatgagcagt tacatccatt tgttttcctg gagcaacaga
    14521 tcccattgta ttttcgttgg catatgtctc tgattttttg tcatctggtc catatacttc
    14581 cacatctgta cctacaggga tatctgaatc gccatcattt tttacggtat aagtaatttt
    14641 tactacttgt gcaggttggt tttcttcaaa ttgatttctt tcatcagtta gttctacact
    14701 atttagtgta tattcagcat caccaacttt tacagtgtca ccaatcttat agaaggtatc
    14761 gcttttttct tctttagaag atgaagcaga ggttgaagat ttagttactt tttcgccacc
    14821 attatcactt gctttattac tatctgatcc accatttaaa gcagaaccaa taattataat
    14881 taaaattact gctaataccc aaaaccatac ttttttgtaa aaaggtttac taactttata
    14941 catttttccg tcttgaccca taactttttt tgccatttaa atattcctca cttcttgtta
    15001 taatatattt gcgatctcag aaatgaggta tgagtccgtg tg
//
LOCUS       DAZBWR010000067        14994 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000067 DAZBWR010000000
VERSION     DAZBWR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.069"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACHGT5_001811"
     CDS             <1..608
                     /locus_tag="ACHGT5_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217793.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACHGT5_001812"
     CDS             628..1656
                     /locus_tag="ACHGT5_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN7217794.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACHGT5_001813"
     CDS             1653..2411
                     /locus_tag="ACHGT5_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN7217795.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACHGT5_001814"
     CDS             complement(2497..3237)
                     /locus_tag="ACHGT5_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN7217796.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACHGT5_001815"
     CDS             3298..4551
                     /locus_tag="ACHGT5_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN7217797.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACHGT5_001816"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACHGT5_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACHGT5_001817"
     CDS             5222..5548
                     /locus_tag="ACHGT5_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN7217798.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACHGT5_001818"
     CDS             5616..6329
                     /locus_tag="ACHGT5_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN7217799.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /gene="licT"
                     /locus_tag="ACHGT5_001819"
                     /pseudo
     CDS             6639..7491
                     /gene="licT"
                     /locus_tag="ACHGT5_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7612..9477
                     /locus_tag="ACHGT5_001820"
     CDS             7612..9477
                     /locus_tag="ACHGT5_001820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN7217800.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9491..10934
                     /locus_tag="ACHGT5_001821"
                     /pseudo
     CDS             9491..10934
                     /locus_tag="ACHGT5_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11042..11743
                     /locus_tag="ACHGT5_001822"
     CDS             11042..11743
                     /locus_tag="ACHGT5_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7217801.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11869..12381
                     /locus_tag="ACHGT5_001823"
                     /pseudo
     CDS             11869..12381
                     /locus_tag="ACHGT5_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12779..12988)
                     /locus_tag="ACHGT5_001824"
     CDS             complement(12779..12988)
                     /locus_tag="ACHGT5_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN7217802.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13229..13804
                     /locus_tag="ACHGT5_001825"
     CDS             13229..13804
                     /locus_tag="ACHGT5_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN7217803.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="ACHGT5_001826"
     CDS             13804..14244
                     /locus_tag="ACHGT5_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN7217804.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="ACHGT5_001827"
     CDS             14260..14961
                     /locus_tag="ACHGT5_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN7217805.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2485 c         3161 g         4282 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAZBWR010000068        14885 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000068 DAZBWR010000000
VERSION     DAZBWR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.070"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1793
                     /gene="yfcC"
                     /locus_tag="ACHGT5_001828"
     CDS             54..1793
                     /gene="yfcC"
                     /locus_tag="ACHGT5_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN7217806.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1950..3134
                     /gene="iadA"
                     /locus_tag="ACHGT5_001829"
     CDS             1950..3134
                     /gene="iadA"
                     /locus_tag="ACHGT5_001829"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN7217807.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3245..3448
                     /locus_tag="ACHGT5_001830"
     CDS             3245..3448
                     /locus_tag="ACHGT5_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN7217808.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3549..4211)
                     /locus_tag="ACHGT5_001831"
     CDS             complement(3549..4211)
                     /locus_tag="ACHGT5_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217809.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4224..5726)
                     /locus_tag="ACHGT5_001832"
     CDS             complement(4224..5726)
                     /locus_tag="ACHGT5_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217810.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6274..7953
                     /locus_tag="ACHGT5_001833"
     CDS             6274..7953
                     /locus_tag="ACHGT5_001833"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN7217811.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8080..8559
                     /locus_tag="ACHGT5_001834"
     CDS             8080..8559
                     /locus_tag="ACHGT5_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN7217812.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8778..9458
                     /locus_tag="ACHGT5_001835"
     CDS             8778..9458
                     /locus_tag="ACHGT5_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN7217813.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9466..10797
                     /locus_tag="ACHGT5_001836"
     CDS             9466..10797
                     /locus_tag="ACHGT5_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN7217814.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10812..11774
                     /locus_tag="ACHGT5_001837"
     CDS             10812..11774
                     /locus_tag="ACHGT5_001837"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN7217815.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11885..12223
                     /locus_tag="ACHGT5_001838"
     CDS             11885..12223
                     /locus_tag="ACHGT5_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN7217816.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12302..13009
                     /locus_tag="ACHGT5_001839"
     CDS             12302..13009
                     /locus_tag="ACHGT5_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN7217817.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13112..14593)
                     /locus_tag="ACHGT5_001840"
     CDS             complement(13112..14593)
                     /locus_tag="ACHGT5_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217818.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4717 a         2550 c         3185 g         4433 t
ORIGIN      
        1 ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata aatttgaaga
       61 aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca ccccatacgt
      121 atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc gttccagcag
      181 gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca agtactcgta
      241 ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg tatgtgtttg
      301 atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat gttccggaaa
      361 aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa aaactagatg
      421 aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc tatgatactt
      481 ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc ggctttggtt
      541 tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct gctgtaggga
      601 tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt accggagcga
      661 tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt ttagctttac
      721 cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca gaagaggtaa
      781 ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac tcgatcgtag
      841 cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg atgagcccgt
      901 ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt gcgacattcc
      961 gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg atatatgcag
     1021 aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat tatttccgtt
     1081 ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat aaattgatct
     1141 tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt
     1201 acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga atcattgctg
     1261 ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc caatctggtg
     1321 cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt cttcttgtct
     1381 tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat gggatcggga
     1441 cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt
     1501 tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg ccaatcatgg
     1561 ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct tatcagcttg
     1621 gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt gtgctaggtg
     1681 ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa ggattcctgt
     1741 ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag tagtactgga
     1801 gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac cgtcatttta
     1861 cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga tcctatcatc
     1921 agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg
     1981 caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt ttggctgtag
     2041 aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca ggtgagggaa
     2101 aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga ggaggagaag
     2161 gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca gctggtgtaa
     2221 cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact gcgttgatca
     2281 gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa ggttcgtacc
     2341 gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca attgataaaa
     2401 ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca agttttgaag
     2461 agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga aaagctggga
     2521 tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag
     2581 aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc acaccagaat
     2641 tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc actgctagcg
     2701 aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa gcactgaaac
     2761 gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat ggacaaggaa
     2821 gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga agtgccaagg
     2881 cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct
     2941 tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg
     3001 aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat attgatacag
     3061 ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg gggactttcg
     3121 aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg ttctgtttgt
     3181 ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa gaggaggaag
     3241 cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag tcggtgaagg
     3301 aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag ggaaagcaca
     3361 atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa
     3421 gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa tcaagcaaat
     3481 aaaaaatccg aaccatatct gcgagacaga tatggttcgg attttttatt tgatcattat
     3541 cctgtttttt aaacttcgat tttttgaaat tttttatttt ttagttcaaa gacttcatca
     3601 cagctttttg ctacttcaat ctcatgagtc acgatgataa tacagcgatt ttgttcatgg
     3661 gcgatttctt ggaaaagttt gacgatatct tgtgaagttg tttcatctag atttccagta
     3721 ggctcatccg ctacgattag ttgatgttca cagcagatag cacgaacgat agcgactcgc
     3781 tgctgctgac ctcccgaaag tttggtcacc tgtttttttg ctaatatttc atcaattcct
     3841 acacgagcca gtcgatctaa agcaacttgt cgataattct cttgcttggt cttagtgatt
     3901 tccattgctg ctatgacgtt gtctagagcc gataaataag gaagcaagtt gtaagcttgg
     3961 aaaataatcg agacatcatt tttacgataa cttcttaacc cgattttgtt cagtgaacga
     4021 tcttgatagt atacctttcc ttcttttggt gcatctagtc cagaaataag cgaaagaaaa
     4081 gtcgtttttc cagaaccact tgctcctaaa atagcgtaca tttttccagc ttcgaaattc
     4141 agttctacat ctttataaag cgcatcttgc ggatcttgat accaaaatcc caattgttct
     4201 gtccgtaaca tcaaattcct cctttagttt aataggattt ttcttgggtt taatcttaat
     4261 atccctatag aagctaaaaa gacagaaaac agactgattc caaatgctac tccggctaat
     4321 agtcccaatt gtgcaggttt gacattgatt tccaattcgc tgacttgatc agaaacagca
     4381 aatggattat tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta
     4441 cctggcattt gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg
     4501 tcaagtagct gctgtcccac ggcattcgcg acgacgtttc cgctaagcga agcaactcct
     4561 aatgcaagaa tcatacaaat agccacttcg gtaaacaatt ggagaatgat tttacttctg
     4621 gattctccta aagatagcaa gacgcctaac tcatgccgac gctcccgaat ggtaatcata
     4681 atgatcagag taagtatgat aataccggct acagccacta gtaaaacaac atttttagaa
     4741 aaacttgcca cgttgtttaa aggttccaac attgactgat acatggaatc atttgattgt
     4801 aagctaaatg tatctgtgtc gatcagcttc tcagcttctt tgagaaactc atccatttta
     4861 tctggatcag atagagtgta aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt
     4921 agttcatttg caaaagtgta agaagtgtaa agtgtattcg acggattcat gaagttgaat
     4981 ttcataccca tactgccgct tgtttcgctg gattcataaa ttcctttgat cgtcatttca
     5041 taagtggtat cttcatcttc agtactcgtg attgtgaaag tatctccgac tgccaggtca
     5101 tttgtttctg ctagagtaga atcaatgact acattgtttg tgcctttgtc gctgtcatct
     5161 attccttcac catccgtgat ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt
     5221 gctgacacac cactgatttg aaagtctgct tgagtcattt gcggattccc gcccattcct
     5281 ccagcaggtt caccgcctgc atcagtagtt tctgaactgt cttcacttga atcagaacta
     5341 gtgatggcag ttattccatc ttttgccata gctgaggcgg aactttcaaa ggaataactc
     5401 tttacattat ctaattcagc tattttttcc gcgtccgtta aggaaactgg tgtaagagaa
     5461 aaactgcctg gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt
     5521 tttttaaatg aagcttctcg attggcactt aaagtcactg ttgctccaac ttctttttgt
     5581 gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag cacaaaaata
     5641 agaatggctg tgaacacggc aaacaataac aatgaacgtc cccattttac ttttgtactt
     5701 ttccatgcac gtttcataaa attcatattt tgcatccctc ctagtagatg catttgataa
     5761 tagacaacct atcttaaagc tagcttaaat cttatgctgt ttgcacacca tcaacatgag
     5821 gttctgtata tataaaaagt cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag
     5881 tatgtttcaa accgacgaag aagaaatgaa agaggaatag caaaagaaat cctagatgct
     5941 acagagggat ttttacgtgc atagttgttc taacaattgc tgatactagc gatttagttt
     6001 atttgttgta taataatggg gaacagctga atacttatat aagtattctg gctatagcgt
     6061 ttcgcaaaag atccattttg ttaatgaagt atatccaaaa taaaatgccc atccaactgt
     6121 cagaacgcgg atgtgctggt aattttcctg ctgattctcc ataagttgag aaatagtgat
     6181 tttgcatatg cttcattgga atggctcata tatttagata aagaagaaaa tcgacatctt
     6241 taaacttgga tgtcataaga agaggtgaag tttgtgagta ccattaaaat tatcccatta
     6301 ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga ggatattttt
     6361 attttagatt gtgggctaaa atatcctgaa aatgagttgt tggggatcga cgtcgtaatc
     6421 cctgatttta cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt tttaacacat
     6481 ggacatgcag atgcgatcgg ggcacttcct tacttgttgt caaaaatttc tgtaccagta
     6541 ttcgggacag agttgacgat cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc
     6601 aaaggattta aggatttcca tgtggtagat gagcatacag aaatagactt tggtgaaaca
     6661 gtagtgagct ttttccgtac cactcatacg ataccagatt cagtggggat caatctgaaa
     6721 acaaaagaag gtagtatcgt ttatacggga gattttaaat ttgaccaaac ggctatccca
     6781 atgtatcaaa cagattatgc acgattggcg gaaatcggca aagaaggagt cttagcctta
     6841 ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag cttctgaagc ccaaatagca
     6901 gatgaagtat ttgatacgat caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca
     6961 agtaatttgc aacgagtgca acaagtattg gatgcagcag aaaaagcaca acgaaaagtt
     7021 gtcttgactg gtcaagattt cgaacgaatt attcgcacag cgatgcgttt agaaaaactt
     7081 caattaccaa gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa
     7141 gaactgatca ttttagaaac tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg
     7201 gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag atttggttta tatcactact
     7261 acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct
     7321 ggtggaacag tgaagttgat ctctgagaat atgcgcgttt ctggccatgc caatcctaat
     7381 gacttgcaat taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag
     7441 tatcgtgaat tagctgctca tgcagatttg gctcatgcag tagggatgcc atacaaaaat
     7501 atctatatta ctggacgcgg agacgtgctg gaatataaga acaagcgtat gagtgttgca
     7561 ggaactacaa gtgcagaaaa tgtaatgatt gatggattag gcataggtga tatcggaaac
     7621 attgttttac gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc ggttgtcact
     7681 atcagtcggc gagaaaagaa aatcatttca aaacctcaaa ttacttcacg gggattcgtt
     7741 tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca atatgattga aacaatcgtt
     7801 gagaaacatt tagaaagcaa tgattttgag tggagcaaat taaagcaaga catccgagat
     7861 caattaggcc gttacttatt tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt
     7921 atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg aacctctcta
     7981 tagagtgctc gtcttgctct ttttttgcga aaaacaaata atttttgaat acagcatccc
     8041 tcgctttata ataaacgata gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc
     8101 gaacgttatt tagaagaaca aaagatcagc tatcaaccac ttgattttag tcatgtggta
     8161 gaagaatctt tcacagaaga actacaaaaa agagggattg atccagcttt gatatgtaaa
     8221 actctcgtag tgaaaggaaa caaaactggg gtgatcatcg cattgattcc tttgaacgat
     8281 cacttagatt acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg ttttcctgga
     8341 atggattttg tattaatgca tacaggatat ccccacggag caaacacccc tattggaata
     8401 aaactcgcac atcctgatta ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa
     8461 gtaattgtgt caagcggtga aatcgggaga agccttcgga taaaagtagc cgatttagaa
     8521 aagatcgtcc atcctattta cgctgattta attagttaag ggacgagaag agaagctaag
     8581 cagagcagag acctcaaact tgagattata tctgcttggt acttggtgca tcctgtatta
     8641 tctgttacaa ttgctttgta catactgtac tatcaatcca atgaaaatat atttagcagc
     8701 tatatttttt cattgatgtg caagtgatga aatcgttttt aataaaaaag atacagagaa
     8761 aacgcgaggt gtaaaatatg aaaattacgt atcatggaca ttcctgttta acaattgaaa
     8821 tggaaaatgg ccaacgcgtg atgattgacc cgtttattac aggaaatcca ttgacagact
     8881 taactgcaga agaagtaaag acagattggt tgctgattac tcatggtcat agtgaccaca
     8941 taggagatat gattcctatt gcaaaagaaa atcaagccac aattatcagt attgttgaga
     9001 ttgctgatta tgcgaacagc agaggagtag attcattcgg tatgaatatc ggtggaaaac
     9061 atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca gcatagttca agctatgaag
     9121 tagacggtat cgttcagtat atgggcgagc ctagcggaat tattattcaa gcagaaggaa
     9181 aaacgattta tcatgcaggg gatactgctt atttcagtga tctaggattg cttgcagaag
     9241 aattcgatat tgatgtcgct ttcttgccga ttggcgataa ctatacgatg gggccagaag
     9301 atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc cattacaata
     9361 ctttccctgt cattgaacaa gatcccgaat cttttgtaga gttactggaa gatagtgaag
     9421 gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat
     9481 gatttgattt tagaatatat tgaaagttta cctgttggca atcgaatttc tgtaagaagc
     9541 atcgcaaaag aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa
     9601 aatgtcggac tagtttcaac aattcaacga gtagggacca tccgtatcga acgcaaatta
     9661 aaaaaacata ttgaacgttt gacatttggc gaggtcgtca ggatcattga aggggacgtt
     9721 ttaggcgggg cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact
     9781 aaacatgcca tggaacgcta tattacgcca ggatcactaa tgatcgtcgg caaccgggcg
     9841 gaagtccaaa aattagcatt ggaagatggc gccgcagtat tgatcacagg aggattcaat
     9901 acgactgctg agattgcaaa gctagctgat gatcttgaaa tgccgatctt acgcacctct
     9961 tatgatacat ttacagtcgc aaccatgatc aaccgagcgt taagcgacca attgatcaaa
    10021 aaagatatca tgcttgtcag tgatatctat atgccagctg aaaagactca ttatttgcag
    10081 cagacagata cgatttctga ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt
    10141 ccagtcgtca accgccatca tcgtttgatg gggattgtta cggcaaaaga tgttttaggg
    10201 aaatcgccaa accagatcat cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa
    10261 acaatgagta ttgcctctgt tagccatcaa atgatctggg atgggctaga agtcatgccg
    10321 gtcgtttccg atgacttgac attggaaggc ttgattaccc ggcaagatgt aatgaaagcg
    10381 atgcagcttg tccagcgaca accgcagatt gcagatacga tttcggatca gatatctgga
    10441 aatatccata cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa
    10501 ttcgtagtag caccacaaat ggtcaatggc gttggaacca tttcttttgg cgtacttagc
    10561 gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg aaatatcttg
    10621 attgaacaag tgaacctaca ttatttgcgg ctgatccaat tggaaagcga attagacatt
    10681 cgtccacgtg tattggaaat cggcagacgt tccgctaaac tggatatcga agtgtttatt
    10741 gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa
    10801 gcaggagtga aatgacagta cagcaagaaa tagtagatac aataaaaaaa tacgataaaa
    10861 tcattatcca caggcatttg cgccctgatc ctgatgcatt aggatcacaa gtaggattag
    10921 ccgaactatt acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg
    10981 aagggcttcg ctttttagca gaaatgcaag aagttgaaga tgtcttctac caaggagcgc
    11041 tagtcatcgt gactgataca gctaattcac ccagaatcag tgatcaaaga tatcagttgg
    11101 cagaaaaact gatcaaaatc gaccatcatc ccaatgatga cccttatgga gatcttgtgt
    11161 gggtaaatac aaaagcaagt agctgtagtg agatgattgc agaatttgct ttgatgtttc
    11221 ctgaatcttt aaagatgaac gttaaggctg cacggttact ttatgcagga atcgtcggag
    11281 atacagggcg ctttttgtat ccctccacga cgtcccgtac attagagatt gcttcgatat
    11341 tgagaaatta tccatttgat gcaagtgctt tgaacagaga gattgaacaa atgccgatga
    11401 aagtcgctaa actttccggt tatctttatc aaaatatcca ggtggatgaa aatggtgcag
    11461 gaagaataat ccttccgcaa gaaattcttg ataaatatgg aatcgatgat tcagagacag
    11521 cttcgatcgt ttcacttcct ggagtgattg acgaagtgct ggcttggggg atttttgttc
    11581 agcagccttc agggtattac cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat
    11641 tggctaaacg ccatcatgga ggaggccatc cattagcaag tggtgcgaat gcaaaagatt
    11701 tagcagaagt agaggaaatc tataaagaga tccaagatat atgccaatcg tataagagca
    11761 gaaaaacaga atgatacttc gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta
    11821 tcttttcaag atcttctttt tccgttacaa tagtgctcac taaagatttt aaaggagaaa
    11881 gaagatgaca ttaccaaatt gcccagaatg tggctcagag tacacatatg aagatcgagg
    11941 cttgttgatt tgcccagaat gcgggaacga atggaacaaa gaagaacaag aaactgtcat
    12001 agaagacgga ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt
    12061 gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa
    12121 agtcaagaac atccgtttag tagaaggcga tcataatatc gattgcaagg tagaaggatt
    12181 tggaccgatg aaactgaaat cggaatttgt gaaaaagaat taaagaatat tcaaggtctt
    12241 ttcacattct tttcctacta ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa
    12301 aatgtatggt tatggaatgt atggtttttt agatccgaca tttcttttgg tcattcttgg
    12361 attagttatt tccggaattg cgtcagcgta cgtcaatagt actttccgaa aatacgatca
    12421 agtgagaagc aaaaacaatg tgactggtac acaagcagca caatatatat tacaaagtca
    12481 acagataaat aatgtcggtg ttcaacaaat tgccggagat ctcacagata actacaattc
    12541 aggtaataag atgttgagtt tgtctcaagc aactgcacag tctacttcag ttgctgcgat
    12601 aggtgtagca gctcatgaat gcggacatgc ggtacaagat gcttcgggtt atgtcccatt
    12661 gaagattcgt gcagcacttg ttccagcagc taactttggt tcgatgattt cgttcccctt
    12721 gattatggta ggtgtattgc ttagctggaa ctctacgtta atcaatatcg ggattttcgc
    12781 tttttcttta gcattacttt tccaattggt gacattacca gtcgaattta acgcgtctcg
    12841 gcgagcaatc caaattttga gtgaaggcgg actactgacg gaagaagaag taccgatggc
    12901 aagacacgta ttatttgcag cagcactgac ttatgttgca gcagcgcttt cgacattcct
    12961 gcagctgttg cgcttgatct tattgttcgg cggcaaccga agagattaaa ataaagaagt
    13021 tgtgaaatca gcgctttgat ctgagctaat accgagagtt agctgattga ataccattta
    13081 tttggaataa gaggatgtga caagaatttt gtcacatcct ctttgttttg ttctcataac
    13141 cttttttcat atacagttca tgaaccgctt ttattaactt gccaaataac actgtattgt
    13201 gcgttgtatc cagatagaaa gtctccgttt catctgaaat tttctcatgc tgatctgtaa
    13261 tgatcaaatc agcatgttct gaatatgcgg taattttaac agcatctttg cgaaataact
    13321 gctgcaaatg gctttgaaga tgataatttc ctgtaaactg tttggttatc tgcaaatgga
    13381 tgagtaactg ctctttcgtt tctgtttgta gtaagtgttg gatcaattga ctaaggtatt
    13441 gactgatttg gtttctttcc ccattttttg aaaaaattgt atcgattttt tctcttaatt
    13501 tcttttcctg tataaaagaa ctagaatgat tggacgtggc ttctggaaag caaagtgtag
    13561 aaaaactact gctggattca cccaacagtt cgaaaaaaac aactgccaac acaagaaaat
    13621 aaaaataaat tggtatttta ttttctaaat ggttgagagg aaactgttga gttaagtaag
    13681 tcatcaattg accgacttgt cgacaaataa ggtttttgct tgtggaaaat aaaaaaccaa
    13741 tctcctgttt ttgtcgaaaa gagatttgat ttggaaaaaa acaatagaaa aaataattaa
    13801 agtaggctct ctctgtttct gtctcttcac aagggcgact cactgaaaaa gaagtaaagt
    13861 attctgaata atcatgtgta tgctgcaata agtccatcaa ttcagtgatt tcagaagaag
    13921 tttttggaaa atatgcctgt tgtttgtttc gtaatatgaa taaagttaac agcaagtaga
    13981 tatttttttc ttgttttgaa attttttcta tttgctcatc tgtactgttc atttttagtg
    14041 catttaattc aagtgaggaa aatggccaat cgttacctcc aaatgtatct gaaagaaaaa
    14101 agtaaagata gagacggata gataactcat ttccttcaag agtacagcaa ttttttacag
    14161 ataggatccg taaatggaat tttattaagg aatgattgag ctgttttgtc agtttttcga
    14221 gataggaggg cgatatataa agttgttcaa gtaaatattt ttttggtaca gcagtttgtg
    14281 gataattgat tagtaacatc aaatagttaa aacgattgga ttcttttagg tagcataatt
    14341 ttaacttaga aaaaacagct aactttttag tttgatcggt ttctttggtt tggatctctc
    14401 ctttatgatt agaataaata aattcatcaa tcaataaaaa ttgcttgagt gatacattta
    14461 tttttcggat cagttctttg attgtccgtt ttggaatatg taagtcattg gataattctt
    14521 gtaaagaaca attatttttt tgaatgacca tttgtagaac aagtaactct ttttttcttg
    14581 cttcaattag cataaatacc tcctttttat tctgtttttg tatatagtta gacaaaaact
    14641 gtgagtggaa agtcatttca gtctgtgtta ttctcacaga gtgtcgcctt cttttttata
    14701 taacgttatt taattttttt gttaccttgt ttattctatt gtttatttcc tagggtattt
    14761 tgtcaagata ttcttgtttt taaggcggaa aaattgattt ttaatggata taactaaata
    14821 tgttttgata attatcaata atatttttag aattatttat gttatttatt tatgggtaga
    14881 tgaaa
//
LOCUS       DAZBWR010000069        14815 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000069 DAZBWR010000000
VERSION     DAZBWR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.071"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHGT5_001841"
     CDS             complement(296..1024)
                     /locus_tag="ACHGT5_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217819.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHGT5_001842"
     CDS             complement(1002..2576)
                     /locus_tag="ACHGT5_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN7217820.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHGT5_001843"
     CDS             2805..3743
                     /locus_tag="ACHGT5_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217821.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHGT5_001844"
     CDS             3755..4666
                     /locus_tag="ACHGT5_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217822.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHGT5_001845"
     CDS             4690..6162
                     /locus_tag="ACHGT5_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN7217823.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHGT5_001846"
     CDS             6213..6824
                     /locus_tag="ACHGT5_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN7217824.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHGT5_001847"
     CDS             6854..8767
                     /locus_tag="ACHGT5_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN7217825.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHGT5_001848"
     CDS             8895..11876
                     /locus_tag="ACHGT5_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN7217826.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHGT5_001849"
     CDS             11873..13534
                     /locus_tag="ACHGT5_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN7217827.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHGT5_001850"
     CDS             13574..14506
                     /locus_tag="ACHGT5_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN7217828.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZBWR010000070        14567 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000070 DAZBWR010000000
VERSION     DAZBWR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.072"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..996
                     /locus_tag="ACHGT5_001851"
     CDS             100..996
                     /locus_tag="ACHGT5_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN7217829.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1146..2087)
                     /locus_tag="ACHGT5_001852"
     CDS             complement(1146..2087)
                     /locus_tag="ACHGT5_001852"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN7217830.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2213..3334
                     /locus_tag="ACHGT5_001853"
     CDS             2213..3334
                     /locus_tag="ACHGT5_001853"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN7217831.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3592..5094
                     /gene="eat(A)"
                     /locus_tag="ACHGT5_001854"
     CDS             3592..5094
                     /gene="eat(A)"
                     /locus_tag="ACHGT5_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN7217832.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5161..5688
                     /locus_tag="ACHGT5_001855"
     CDS             5161..5688
                     /locus_tag="ACHGT5_001855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN7217833.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5899..6960
                     /locus_tag="ACHGT5_001856"
     CDS             5899..6960
                     /locus_tag="ACHGT5_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN7217834.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6963..7859
                     /gene="murQ"
                     /locus_tag="ACHGT5_001857"
     CDS             6963..7859
                     /gene="murQ"
                     /locus_tag="ACHGT5_001857"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN7217835.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7873..9855
                     /locus_tag="ACHGT5_001858"
     CDS             7873..9855
                     /locus_tag="ACHGT5_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN7217836.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9920..10366)
                     /gene="nrdI"
                     /locus_tag="ACHGT5_001859"
     CDS             complement(9920..10366)
                     /gene="nrdI"
                     /locus_tag="ACHGT5_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN7217837.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10577..12001
                     /locus_tag="ACHGT5_001860"
     CDS             10577..12001
                     /locus_tag="ACHGT5_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN7217838.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12182..12955)
                     /locus_tag="ACHGT5_001861"
     CDS             complement(12182..12955)
                     /locus_tag="ACHGT5_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN7217839.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12952..13857)
                     /locus_tag="ACHGT5_001862"
     CDS             complement(12952..13857)
                     /locus_tag="ACHGT5_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7217840.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14011..14331)
                     /locus_tag="ACHGT5_001863"
     CDS             complement(14011..14331)
                     /locus_tag="ACHGT5_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN7217841.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4646 a         2602 c         3092 g         4227 t
ORIGIN      
        1 gttaaatttt catacaaata taggaaacgt tttactggct ttttactatt taaacaggct
       61 atcataggaa aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg
      121 ctagttgcaa aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg
      181 tccggaaaag atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat
      241 tatcagctga tcgttttttt agattatgga tttgatatgg agatggctcg acgcgtgcgc
      301 ccatttacca atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag
      361 gcgatgctga agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt
      421 ttggaagcaa aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatgaat
      481 ttttcttcta gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa
      541 gaacgagcag aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc
      601 attcttccac atcgagggaa tgaacaacct ggatatctta cttatgaaga gtacctgaaa
      661 aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact
      721 ttgcggacat ttgaatccat gtattttcaa aagaaactag tgacggataa tcaagcgatt
      781 gctcattatc atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat
      841 gaattaccgg aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt
      901 tatgatgcag aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa
      961 tacgaatatg acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta
     1021 tgtactgaaa caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga
     1081 tcttttattg ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga
     1141 gccttttatt ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca
     1201 caatatggtg tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag
     1261 tcgatttcag tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac
     1321 ccgatgttgt caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt
     1381 atgattcctt cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc
     1441 tctgccgctt tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca
     1501 ttcaatgttc catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag
     1561 atcggattta aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct
     1621 tctggttcca agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga
     1681 ccagctagac cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat
     1741 ttttccagta catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca
     1801 gtaataatgg attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct
     1861 acgatcaagt ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt
     1921 tttgcgacca cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca
     1981 atgtgagcag gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat
     2041 ccgattcggc tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc
     2101 tttattcaat gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt
     2161 ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat
     2221 tttggcggca attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt
     2281 tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg
     2341 agaaaatact gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg
     2401 gatggatctc ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa
     2461 aaacaccgca gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac
     2521 ggatcaaact atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact
     2581 gcttcaagaa gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg
     2641 tctaggatta ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt
     2701 attatctact aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt
     2761 gattatcgct gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt
     2821 tggaagacaa aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa
     2881 gattgctcag caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg
     2941 agcagcaggc ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt
     3001 cgatttagtc agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat
     3061 tacaggagaa gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc
     3121 agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga
     3181 gctagaagga ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg
     3241 tttagaagaa gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac
     3301 acttagcaga tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta
     3361 tcgtaatttc aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg
     3421 atgaaagctg cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg
     3481 gattggcttg gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc
     3541 tttagccagt tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa
     3601 atcgaaataa aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa
     3661 gcaaatctaa attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga
     3721 aagacaactt tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat
     3781 cagcaagaat ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac
     3841 gtgttaaatg atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg
     3901 caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca
     3961 aaagtcctac tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca
     4021 acgaatcatt tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa
     4081 caaggcttca tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt
     4141 ttagcaatcg aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa
     4201 cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc
     4261 agtaggctaa agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat
     4321 aaaacaaaga aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca
     4381 gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag
     4441 acccagatca gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg
     4501 atgaatagcc aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg
     4561 tatgaaaatc tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg
     4621 atttcaggtc ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc
     4681 aacggcaagg ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc
     4741 caaaattatg aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac
     4801 taccaagcat ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac
     4861 aagatcgagc agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca
     4921 cagccagctg aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa
     4981 gaacaattag aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat
     5041 caaaccttcc tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg
     5101 taatctcaag aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac
     5161 atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga
     5221 cgggcagatg tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag
     5281 gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga
     5341 gatacagcac aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg
     5401 gtagaaatgg attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa
     5461 gcacgtttag cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca
     5521 cggatcgttc ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt
     5581 tgccataatg ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg
     5641 ttctttacat tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt
     5701 gttatttaaa taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta
     5761 tgacaattct tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata
     5821 aaagtattga aattttgttt ctttagatat atactctagt catagaaatg aagacggata
     5881 cagaaaaaga gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa
     5941 gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc
     6001 atgcatatac cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc
     6061 gaagcgaaaa agaataaact agaattgatg gtcgatattt caggagaggc attggaacga
     6121 gcgggttttt cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga
     6181 atggattatc atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt
     6241 ttgaatgcta gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat
     6301 ttctcacaga ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa
     6361 gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt
     6421 gtaccaggag atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa
     6481 catcgaggga tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt
     6541 gtttacatcg gagatgcagg tctgtcagaa aacgttcagg agcaatttgc ttctttccaa
     6601 aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagaatttta cgaatatata
     6661 ttagggaagc atacgaatcg gcaagacgat gcaagagatg tcatacgaag cgtagatgca
     6721 cgttttcgag agattccgcc tatcccagca agaaatacag ctacgcgtat gaaagggtct
     6781 attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aaccaaatat
     6841 gatttgccag cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga agagctacca
     6901 ttgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa
     6961 aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg
     7021 gattagatga gatgagtatt tctcaagccg ttcaactaat gaacaaagaa gatcagaaag
     7081 tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct aacacgattg
     7141 agtcctttaa aaaaggtgga cgtctgattt atatgggagc cggaacaagt ggaagactag
     7201 gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa atggtcatag
     7261 gtctaattgc tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc gaagattctt
     7321 atgagctagg gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat atagttattg
     7381 gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg attagattac gcaaaatcga
     7441 tcggtgctgt cacaggaacg atttcttgca ataaacaagc cgaaatcagt aaacacgcgg
     7501 atttcccaat cgaagtcgat gcaggtccag aatttttgac aggctctact cgattgaagt
     7561 caggaacagc acaaaaactt atcttgaata tgatttctac tatcagtatg attggtatag
     7621 gtaaagtatt caataacttg atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac
     7681 gctcaaaacg aatcatcatg caagcgacag aagctgatta cgaaacagct gaaaagtact
     7741 ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat gattttgaca aatagtgata
     7801 aagaggaagc tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg accttatgag
     7861 ggggaaaaga ccatggcaaa aaacgaactg actttggaag aactggcaaa acaaatttat
     7921 aaaggggcag gcggcatggg caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt
     7981 atgaagatca tcaatgatga tcttgtaaat gtctctgaat taaaagcgat tccaggagtg
     8041 cttggtgtag tagaagatga acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa
     8101 gtagcacagc aaatggttga tttggcaggc gtgaaattag gagaaaaact ttcttctgta
     8161 agtacagcta cagcaaattc atcagcatct ggaaaagacc gtgtaaacga acgggcacaa
     8221 gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa
     8281 gacatttcta atatctttgt gccaatgatt cctgcatttg tcggtactgg gatcgtggca
     8341 gggattgccg cagttttagc aaacttagtc acagccggaa cacttgatgc agcaacatgg
     8401 caacaatata tcgatgtgat gaacatcttg aaaaacggga tgtttagcta cctagttatc
     8461 tatacaggta tcaatgctgc acaagttttt ggtgcaacac caactcttgg tggagttatc
     8521 ggggccgttg tgatgctgac agggatgaat ccggaagcac cactgaaaaa cttatttact
     8581 ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc tgtttggcta
     8641 ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt
     8701 acaccaactc tttcattgat tgccatcggt ctgattgaaa ttttcttcat catgccttta
     8761 gcaggtttca tttctgatgg catggtcggt gggatcaact gggtacttgg cgttggtgga
     8821 gcatttgccg gcttcgttct aggcacatta ttcttgccga tggttatgtt cggactgcat
     8881 caaattttga ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt
     8941 ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag cattggcatt atggattcgc
     9001 tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag ctttaccagt cgggatccta
     9061 ggaatcggcg aaccattgat ttacggagta acattacctc taggacgccc atttattact
     9121 gcttgtatcg gtggaggtat cggcggggca gtgatcggaa tgatcggaaa tgtcggggct
     9181 atcgctattg gaccttcagg agccgctttg atcccattga tctctgacgg taaatggtta
     9241 ggctacatcc ttggattatt ggcagcttat gctggtggct tcgttgctac attcttcttc
     9301 ggtattccta aagaccgttt agaaaaagaa gaattagcga ctgaaacagc cacagtaaat
     9361 gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa gtgtgactat cctctcatca
     9421 gttgctgacg gaacagttgt ttctttagat aaagcaagtg atccagtttt tgcacaaaag
     9481 atgatgggtg aaggctactt tgtagaacca gaaaacggac atatctattc tccagttact
     9541 ggggtagtca gctctgtctt cccaacgaaa cacgctattg ggatcacaac gccaaatggt
     9601 ttagaaatct tgcttcatat gggaatcaac acagttgact taggcggtac gccatttgat
     9661 gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc ttgtagcaga agctgactta
     9721 gaagcgatca aagcagcagg caaagaaaca tccatgatcg tcttggtaac caatatggat
     9781 cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg
     9841 gatgtcaaag cttaatatga tagaaacaat agaaacgaaa gaagaaactg aaatctattt
     9901 ttcagcttct tcttttttat tatttattgt tttctgctaa aatctgataa attcgtgtca
     9961 catcagatgg tgttccccgt aattcataat ctgccaagaa aggaaagcca aatgtttgtg
    10021 catattgttt ggctgttaga caatactgat tattgaagtt tttatttccg ctaccgacta
    10081 caccaagaca aagtttatga ttgtcttcat gttccagata ttcacgcatc gtttcggtca
    10141 agatttctgt atcaccgtta tctaaaccat ttccgccgtc taaataagta ggaacgaaag
    10201 taaaaaatgg ttctgtttct ttcgtaaaat cactattttc atggatttct tttaaagtaa
    10261 cttcaggatt gacaggatct tctgcatgca ttttttcagc atattctgct agatgtttgg
    10321 caaaggcacg cgtatttcct gaaatcgata tatataaaat gttcatggat catcctccaa
    10381 ttctttcctt ttagtatagc atctttttta agttttttgg tggtgtaatt tggaaaggga
    10441 ctaggcgctt tgcttgtata atgagaaaaa agccggtaga attatatttc tattaaagtt
    10501 tttattaatt aagtaaaaat aaaaaaggtt tgttatactt tttgataaaa gcggttttat
    10561 tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt taattccact
    10621 tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta tgacggacat
    10681 ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa cagcaatgtt
    10741 gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa aaggcgggat
    10801 ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg gttggggtta
    10861 ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga gcgcattagg
    10921 atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga tgagtacagt
    10981 ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta gtttcgtcaa
    11041 tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag tagctatcag
    11101 cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat tgaatgcgaa
    11161 tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga tgctttgggt
    11221 atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac aatcagatgt
    11281 tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct tggtttccat
    11341 gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc agcctgctat
    11401 gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca acggcggttt
    11461 gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg aaacaatggg
    11521 aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca aaaatggggc
    11581 accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac tttcattatt
    11641 attcactgac agtgcttata actttgctta cacacttggt acagcagcta ttttcttcac
    11701 gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag aatgggtcca
    11761 atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag tcgtagcctt
    11821 taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt acctcaaagc
    11881 acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt tacttatagg
    11941 tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc ttcacgtata
    12001 gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa aaacatacta
    12061 ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa cggtgtttaa
    12121 gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt ttgatgagtc
    12181 atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa acgatgtaag
    12241 ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc atgatctctt
    12301 cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg atccactccc
    12361 atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa taaaagacga
    12421 ttcctgctga gatggatgcg ccggaactat tgataaagca agcaatcatc aacactaaac
    12481 ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag ctttggatca
    12541 gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga atcagtttca
    12601 tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata actaatgtga
    12661 tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga tttttcaatg
    12721 taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg ataaagacaa
    12781 tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg atcgtcgtgt
    12841 attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca gataaaacag
    12901 cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat gtcatttttc
    12961 ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat cattcgtcag
    13021 atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca cggttcctaa
    13081 taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag tatatccagc
    13141 ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga tgaaatcttg
    13201 cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt gattgatcag
    13261 gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga tcaagaaaga
    13321 ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg cttgtggatc
    13381 caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta aggcaagagc
    13441 aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt gaatgtactt
    13501 ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg tttcagagaa
    13561 taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt cgatcaatgc
    13621 tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac caatacagat
    13681 tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg tttttccagc
    13741 gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac tgacatcgct
    13801 gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac ttttcaaaaa
    13861 tattcactcc ttttctatag atagtacaag catacacctg atgagtggag aagtcgctag
    13921 gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata aaaaggctta
    13981 ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct tgatgccaat
    14041 gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct tatcatgaaa
    14101 caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga taggataaga
    14161 gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag caatcatgcc
    14221 aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt tcatcatcgt
    14281 tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca tacttacatt
    14341 cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttcaa agacctaatg
    14401 gaatgatcat cctctttatc ctacccggtg gcagattgta tatctttttc tgtagtatag
    14461 cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca agcactgacg
    14521 gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatag
//
LOCUS       DAZBWR010000071        14269 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000071 DAZBWR010000000
VERSION     DAZBWR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.073"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..480
                     /locus_tag="ACHGT5_001864"
     CDS             <1..480
                     /locus_tag="ACHGT5_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217842.1"
                     /translation="YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQL
                     LLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATV
                     LKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(747..1916)
                     /locus_tag="ACHGT5_001865"
     CDS             complement(747..1916)
                     /locus_tag="ACHGT5_001865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN7217843.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2120..3295
                     /gene="mltG"
                     /locus_tag="ACHGT5_001866"
     CDS             2120..3295
                     /gene="mltG"
                     /locus_tag="ACHGT5_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN7217844.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3431..3910
                     /gene="greA"
                     /locus_tag="ACHGT5_001867"
     CDS             3431..3910
                     /gene="greA"
                     /locus_tag="ACHGT5_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN7217845.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4079..4810
                     /gene="liaF"
                     /locus_tag="ACHGT5_001868"
     CDS             4079..4810
                     /gene="liaF"
                     /locus_tag="ACHGT5_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN7217846.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4807..5874
                     /gene="liaS"
                     /locus_tag="ACHGT5_001869"
     CDS             4807..5874
                     /gene="liaS"
                     /locus_tag="ACHGT5_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN7217847.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5881..6513
                     /locus_tag="ACHGT5_001870"
     CDS             5881..6513
                     /locus_tag="ACHGT5_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN7217848.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6653..7318
                     /locus_tag="ACHGT5_001871"
     CDS             6653..7318
                     /locus_tag="ACHGT5_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN7217849.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7346..7639
                     /locus_tag="ACHGT5_001872"
     CDS             7346..7639
                     /locus_tag="ACHGT5_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN7217850.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7707..8654)
                     /locus_tag="ACHGT5_001873"
     CDS             complement(7707..8654)
                     /locus_tag="ACHGT5_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN7217851.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8832..9635)
                     /locus_tag="ACHGT5_001874"
     CDS             complement(8832..9635)
                     /locus_tag="ACHGT5_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN7217852.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9781..10695
                     /locus_tag="ACHGT5_001875"
     CDS             9781..10695
                     /locus_tag="ACHGT5_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN7217853.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10743..11213
                     /locus_tag="ACHGT5_001876"
     CDS             10743..11213
                     /locus_tag="ACHGT5_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN7217854.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11298..11906
                     /locus_tag="ACHGT5_001877"
     CDS             11298..11906
                     /locus_tag="ACHGT5_001877"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN7217855.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12040..12240
                     /locus_tag="ACHGT5_001878"
     CDS             12040..12240
                     /locus_tag="ACHGT5_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN7217856.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12994..14220
                     /locus_tag="ACHGT5_001879"
     CDS             12994..14220
                     /locus_tag="ACHGT5_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN7217857.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4874 a         2167 c         2856 g         4372 t
ORIGIN      
        1 tattcatcag tagttaacat ggtgaaagaa atgaattttc aaggaactta ttctaatgaa
       61 caaatggaaa ataaacttga agaatttgag aattttgatt atgtacctat aggctataaa
      121 aaagatcaat tgcttttgaa aaaccatcaa gttacagaag atggtcaagt tatttatagt
      181 agcttttctc caaataaaga tgaaattaca tttacgttta aatcttcaaa aggcaaaaca
      241 atagttttac ctgtattccg gtataaagga ttaacggtta ctgttaattc aaaaaatgct
      301 acagtactaa aacaaggagg tccttttgtt acagtaaaaa gtgaaagtgg aataaataaa
      361 gtaacaatca ggtattcata cacatttttt tctaaaatct cattatttat ttctataatc
      421 acgctaattg ttcttctttt ttattgcata aaattaaaaa taagaacaag ccatgaatga
      481 tttgctagca catattttag caatctaaag atactatttt agatttaata ggccctttgt
      541 caaataatgt tggtaaagag aaatcttaga ataatttgga cagaacagaa agaagaacac
      601 ctctttctgt tctgtttatt tggaattaaa gtctgtagca aaaattgatt tttagatttg
      661 tcccacactc tttcctacgt ttagtcgagt acttttttta agcaaatgct attcaaagaa
      721 gtagtatttg ctatcttttc atcatatcag ctatttgcca acttagtagt tccttccatc
      781 aggtcataga tttggtttct tagatcagca gtaggcagtt tgatagtttt gtcagctttt
      841 atattgcttt ccatgatgat agcatcctgt gaatctttgc taaatatagc agatgtatca
      901 aaagagtcga tatttccttg cgacaggata taattatctc cgctatatac cccaccacta
      961 tatggatgtc cattcgcatt ttcacgccat aatgagaaga gtaagtcttt ggaaactagt
     1021 tttccagata aaacttgtgt aaacagcgta tttaaatcat agacggacat actgatatta
     1081 ccagtcccta attcgttatg gatctctgtt tcagattctg tcagtggttt aaagtaatca
     1141 tcctctttag acatttgata ggatagtgca tgattggatg aatttttgac ttggtcataa
     1201 aattttgtat gctgtaatcc taaaggttcg atgatatcat cggtcaataa tttttcatac
     1261 ggttgatttg ttaatttgat caaaattcca gtcaatatgg taaagttcaa tgaatcataa
     1321 tgagaggtct gataatctgt aaattttaag tgctgaatcg caaattgaac gacttcttca
     1381 tcagtcatcg gtatggttgg agaagcagtt cggtgcaagc ctgagcgcat atacagcaag
     1441 tcacggatag taatttgctg gctgccagca atttgtggat agaattgagc cagcttagta
     1501 tctaaagaaa ggtgcccacg ctcgacttgt tttaaaatca aaagagtagt caatgctttt
     1561 tgaaccgaac caatctgata ataaagagaa ggatcatttg gttgttgcgt gagtttattc
     1621 gcatagccat agcctttttc catgatgatg cgattatttt tctttacgta aattgagcca
     1681 atataatcat tttttcttaa tagttgatcg attttttcaa cgagctgttg ttcattggta
     1741 gtcagtatca actttttttg agctgattga gcatgggcaa ctatctgaga gtgttcatta
     1801 ttgtaaaatt gccatccaag aatagttcct gtagagaacg cactaaggct aaacaacacg
     1861 acaacgatca ttaaaaatga atattttctt tttttagcat gttttccttt tttcataata
     1921 gtttatttag tctcctttct tttttacaga aagaataaag ctaaaatagc ttgtatcttg
     1981 ataacattta ttttaatctt atagtaatgg atttactact tacatttttg atagaatatt
     2041 taaataaaat cattcacgcc aattcaaata tcatgtataa tgaataaagt tacgaacttg
     2101 atagtaagga ggagttgttt tgtcaggaaa caaacaacac ccaagtaaag ataaaaaaga
     2161 agccagtaag gcagctacag cagatataat cgacaagcgg agaaatctgc gtaaaaaaga
     2221 agataagatt gtgaggaaga tcattttagt gattgctctt acattattga ttattggcgg
     2281 atttcttgga tttaccgtgt atcgttatgt agatagcggg ttgaagcctt tagataaatc
     2341 agatgaccaa ttagtccaag tcgagatccc gagtggctct tctaacaagc agattggtga
     2401 gattttggag aaggacaata ttattaaaag cgggattgtt ttcaattatt atacaaaatt
     2461 taaaaattta actggctttc aagctggcta ttatcagtta gcgcctaata tgactcttga
     2521 cgagattggc aaacagttgc aagaaggcgg tacttctgag ccaacaaaag ttgcagatgg
     2581 gaaaattgcc attccagaag ggtacgatat cgatcagatt gctgaacggg tagcgaaggt
     2641 aactggtaaa gacaagaaag aatttttaga tttagtgaat gatgagactt tctttaacga
     2701 gttgcaccaa aaattcccag aactgttgga aagtgcatct aaagctgaga acacaaaata
     2761 ccgtttggaa ggttacttat tcccagccac atatgattat tacacgagta cttcgcttaa
     2821 ggatctagtg attgaaatgg tgaacaagac gaatacggtc atgcaaaact attattcagc
     2881 tatcaagcag aaaaatttga ctgtacaaga agttttaact ctagcttctc tagttgaaaa
     2941 agaaggcgta aaagaaaatg atcgtaaaaa cattgcacaa gtcttcttta accgaatcaa
     3001 agcaaatatg ccacttcagt cagatatctc tgttttatac gcattaggtg aacacaaaga
     3061 attagtgacg tatgaagata cagctgtaga ttcaccatat aatttgtata ccaataccgg
     3121 ctatggacct ggaccttttg acaatccaag cgaagaggca atcaaggcag tattagaacc
     3181 agcagaaaat gactattatt atttcgtagc agatacgagc actggaaatg tttatttcgc
     3241 taaaacttat gaagaacata tggaattagt ggaaaaatat gtaaataatt cttagaaaaa
     3301 caggctctag acattgatca tttttagaaa aacatttaca tttgaagaaa agcgtggtaa
     3361 tcttttttgg actatattcc tcaaaagatt accttttgcg gtattttatc gaaaaaagga
     3421 gacgttacac atggtagaga aagtttatcc aatgactcta gaaggtaaag aaaaattaga
     3481 aaaagaatta gaagaactaa aaacagtcaa acgcggagaa atcattgaac gaatcaaaat
     3541 tgcccgcggt ttcggagatc tttccgaaaa ctctgaatat gaatcagcga aagatgaaca
     3601 agcatttgtt gaaggaagaa tcacaacaat tgaaaatatg attcgttttg ctcaaatcat
     3661 cgataacgac ggtgtagatg cagatgaggt atcaattggt aaaacagtta cattcatcga
     3721 actaccagat ggagaagaag aagagtacac gattgtcgga agcgctgaag cagatccgtt
     3781 ttctggcaaa atttccaatg attctcctat tgcgcaagca ttgattggca aacgtaaaaa
     3841 tgatgaagta actattgcta ctccaggtgg cgatatgtta gttaaaatcg tcaaagtaga
     3901 acaagcgtag tctgactgaa agatctagag agttcatctt actgatgata cactctagat
     3961 ctttttctct ttgtgtattt tagatttcaa caaacgatct tacatctaaa gtcctatagc
     4021 caacagttaa aaaaataggt atgatgaaat gagaatgtgt caggagggag aaagagcgat
     4081 gaatagttct tggcgttttt ttgtggtagt tgaagcacta ctgctgttat ttgctgtttg
     4141 gcaaatcgta aacaatactg gacttttgct tttagtgata ttcggtattt tcaatatata
     4201 tctagctatg cgcaaacatc ctcgtactaa atttcaaaat tttcaattga tattaggaag
     4261 tttggtcatt ttttttagct tagtcaacag tcctgcttta tggatgatgg ctgtatttgc
     4321 cgttttattt attggtctga aaggtgtaga gatttcaggg atagatttaa ccaaaaatgc
     4381 tttttggcga aagaaacaga tcatgatgat ccaaacagag caattaaaaa cacataataa
     4441 tgagcggaaa agacagcaat tattcggtaa ccaacgtatt ggaaacgacg tctatgaatg
     4501 ggacgatatc aacatcgccg tcatttctgg tgatactatc atagatttgg gcaatacgct
     4561 tttgccaaaa gatgataata ttgtgattgt aagaaaaggg ataggcagaa cacggatctt
     4621 agtacctttg ggcgtagcca tccgattaga gcatgcgaca ttagtaggaa atgtcttgtt
     4681 tgaagaagag caattttctt tgaaaaatga acaaatcaaa atctacagca gtgattatga
     4741 tgaaaaccct cgtagattga agattattac taataccttg attggagatg tcgaggtgat
     4801 acgaatatga tgggaaaaat atccagagcg atgctagctg tttattcggg gattgcggct
     4861 ttccttatta ttctattttc acttttcact tatttttatg ccagcaatca aagtcattgg
     4921 tggggagaat tactgcgtgc acgtttatta tatgttccgc ttatcttcca tttgttagcc
     4981 atttccttag gtgtaggatt aattgtcttt ctattattat cacttattca aaagacaaaa
     5041 tacgggaaaa tcgaagaaaa gctgcgtgca ctttcttctg gcaattatga atccaaattg
     5101 ttgcttcttc cgatcccaag tgcatcggac gatttataca tcaaagatat tgataaggaa
     5161 atcactaaga taaaagaaaa aatgattgaa gtatctagtg aattacaaat tgtaacgagc
     5221 cgtccccaat atgtagatgg acaaaccaaa gaagaaattt tagaattaga aagacatcga
     5281 ttagcccgtg agctgcatga ttcagtttcg caacaattgt ttgcagcaat gatgatgatg
     5341 tcggcattga cggagcaagc agaaaaaagc gagacaccag aaatgtttcg caagcagttg
     5401 aaaatggtag cagagatcat caacgcttcc cagtctgaga tgcgtgcgct gcttcttcat
     5461 ctacgtccag tcaatttaga agagaaaagt ttaaagcaag gtatcgagca gttattgaag
     5521 gaattgcaaa ataaaatcca gatttcgctg aaatgggatg tagaagatgt aaaactatct
     5581 agttccattg aggatcactt gttccgaatc gttcaagaat tgttatcaaa tacattaaga
     5641 catgctaaag ccaatgaatt agaggtatat ttgcacaaaa tagacaataa tcttttgtta
     5701 cgtattattg acgacggaac agggtttaat atgaatgaaa cgaaaacggg aagttatgga
     5761 ttgaacaata tcaaagaacg agtagctggt attggtggaa cagtaaaaat cattagtttt
     5821 aaaggtcaag gtacaagtgt agaaatcaag gtccctttga tgaaggaggc atagataagg
     5881 atgataaaag ttttattagt agatgaccat gaaatggtgc gcttaggcgt ctctttttat
     5941 ttatctattc aagaagacat tgaagtaatt ggagaagctg aaaatggacg acaaggctat
     6001 gagaaggcga tgacacttcg accggacgtc attttaatgg acttagtcat ggaagaaatg
     6061 gatggtatcg aatcaacaaa agcaatctta aaagattggc cagaagctaa aatcattatc
     6121 gtaaccagtt ttattgatga cgaaaaagtt tatcctgcca ttgaagcagg tgcagcaggg
     6181 tatttgttga agacctctac cgcacatgaa attgctgatg ctataagggc aacgcaacgt
     6241 ggcgagcgag tattagaacc agaagtgaca acaaaaatga tggaaaaaat gagtcggcga
     6301 aatgagcctg tattacatga agaactaacg aatcgggaaa atgaaatttt aatgctgatt
     6361 tctgaaggaa aaagtaatca ggaaatagcg gacgaattgt ttattacact gaaaacagta
     6421 aaaacacatg tttccaatat attggctaag ctagaagtgg aagaccgtac acaagccgct
     6481 atctatgcat tcaaacatgg tttggtgaaa taaaaacgat gataaagagg ataatcaaaa
     6541 gctgagaaaa taactattat ttttcttggc ttttttacaa taatgatgct tgcttttgtt
     6601 tttgtaaagg ctttgttata ctgagaaaag aagatactaa aggaggaaaa cggtgaaaca
     6661 aaattttgca attgtaggtc ttggacgatt tggcggaagt atctgccgaa cactgatcga
     6721 agctggtcaa gaagtactgg ctattgatag taatgaagat cgggtcaatg aatatatgaa
     6781 tatagccact catgctgttg tggcaaatgc acaggatgaa atgacacttc gatcattagg
     6841 cataagaaat ttcgatcatg taattgtcgc tattggagaa gatattcaag ccagtatctt
     6901 agttactctg atggtaaagg aaatgggcgt accaaatatt ttagcaaaag cccaaaatga
     6961 gtatcatgcc agagtacttg aaaagatcgg agctgatcgc gtcgtccatc ccgagcgaga
     7021 catgggaata aggattgcgc ataatttagt gtcaaagaat attttagatt acctagagtt
     7081 gtcagataaa tattctttag cagaaatccg agtctccaat cctaaatttt tcaacaaaac
     7141 attagctgaa ttaaatttca ggcaacgttt tgacttaaca gtagtcgcca tacgaagagt
     7201 agatcaaacg gttatcgctt ctcctgcagc agatgaatat gttcgtgaaa atgataatct
     7261 actggttatt ggagagacag gcgatgtgga tattctagac gataagatga atcaatagaa
     7321 agaaaagaga aagtaggttg ttgggatgaa attaacggta acgccaaaag cacaaaagtg
     7381 gtttgactca gaaattgatc tgccagaaac ttacggaata cgtttttttg gaaaagtcta
     7441 cggcaaaaca gaggttcatg atggtttttc gattggaatg tcagttgaac agccagaaca
     7501 tcctgtaaag aaagtggaaa ttgatgggac actttttttt atcgaggaag cagatgaatg
     7561 gttctttaaa ggatatgatt tacttgttga ttatgatccc aacctggagg agccaaccta
     7621 tcactttaaa aaacaataaa aaaagagagg gtgacacaat ttttgtcacc ttctcttttt
     7681 tcttttgaaa atattgtttt cagctattaa tatcctgcag acaattttac ttgattggtt
     7741 tgaagctcat gggtggcaaa gtagcttttc agattggtta aaaaaatagc cattaatttc
     7801 ttttgaaaat caggtgtcaa acctgcaatg tgggaagtaa tcagtacatt gtccattttc
     7861 caaagaggat gatcttgtgg aagaggctct tcctcaaaaa cgtctaatgc tgcaaaggaa
     7921 agttgctggt tatttaaagc ctgcaccaga tcttgtgtct taacagaagc accacggcca
     7981 acattgatga atacagattt tggatcaaag tgttcaaaca tatgctgatc aaagagccca
     8041 gttgtttcct ccgttagagg taagatattg actacgaaat ctacttcagg aagaacttcc
     8101 tttagttgat ttaaagaaaa agtcttttcc attccttctg ctggatgacc ggatgtattg
     8161 attccatagc agttcatgtc taatgctttg catttttttg caatcatttt tccaatatgg
     8221 ccagttccaa tgattagtat ttttttccct tggatagatt ggtaatggac cgatgaacct
     8281 aaccatttca tgtcactctg tgccttttgt gcttgaaaaa gaccacgaga atacccaagg
     8341 atgattccta agacatgctc cgtaatcgat tcactgtgga tgccactacc atttgaaagt
     8401 aaaatatgtt tctttgaaaa agtctcaagg ggtagctgat caacaccagc tgagatagat
     8461 tggacccatt tcaaggtaga agaggctaat agtttttctt ggtagtctga gttccagccc
     8521 aaactgatct ctacattttg tagatcctct ggttgtaatt ctgttttaaa gatataacca
     8581 ggcgctatag tatggatttc ttcgataaat tcatcacgaa atggacgatc tgcataaatg
     8641 attctttctg acatagttaa tttcccgcct ttctttactc taagtgtagc gcatacaaga
     8701 agaactttga agcgccttgc ttgttttttg agaaagaaat atttttcaaa taaaaaaggg
     8761 gctgtgacaa aaaaatgtca caacctctaa aagggtctat tgaagtagaa ataacgagtt
     8821 gtctttattt cttaattgat cgatattttt gcccatcctt tgacagcttg agcaaaaata
     8881 aagattcctg caaaagcaat gaaggcgcta gtgtcaaaag aaggaagaaa gaccatcaag
     8941 attgttgcaa ttatagtagg caatgaaatt gaatagatca atgtttttaa acaatcaaga
     9001 aaagtagctt ttgttagtcg aaagcgtgca tagagaaaag cagcaatgtt ggtaaatagc
     9061 aaagtgatca gcagattcag aaaactagga taaatagaaa atataaatgt caggactttt
     9121 atccaaagtg gtatagatgc ttcacttaaa aaagaacgga gctgcttacc tgttaagttt
     9181 tttaaagatt cattggtgta gggaagttct aattggttat tgttcaacaa agcagaagtc
     9241 gtgcctgtat taggaagagc tatgataagt ttgtttttta acatcccgac actaacaaaa
     9301 ttccccatca aatcagtaga gatgtcctgt tctgttcgtt ttccctctgg atcaaatgtg
     9361 aagataatgg agtttgtttg gtaaatgaat ccttcttttt cttcggtttg aagctgcccg
     9421 ttttggattg taaaatcagg aatgcgagta gcaatctttt gcccatccaa ttgaatgttg
     9481 tttaagactt gaaaaacttg aaaagagatt ggcaaagcca taattatact caaaactaaa
     9541 agatagacaa tggctttcca aaatgggaca tttctcgcct cctttaggtc tttgaatcgt
     9601 ataaaggaac tgataattaa ttgttttgtt gtcataagta ctcctcatct agtagtgtca
     9661 tcacttgcaa ttctagcgaa gtatcaagtg aaatacaagt gctttaagga aacagctgaa
     9721 tgattggtga aatatcaaaa aaatgttatt cttgtctaaa gactatccaa tataaataaa
     9781 atggaggaag gaatagtgcc tacacagcca cagagatcat caaaaaaact atttggagta
     9841 gtggttttga atgcggtgat cactttatca gaatttattg gagggattat gtcagggagt
     9901 ttgtctcttg tttcagatgc tttccataac cttagtgata cattggcaat tattttttct
     9961 tacggggcac aaaaaatggc ccgaaaagaa gcaaataaaa aacgtacgta tggctaccag
    10021 cgtttagaaa tattgagtgc tttcatcaac tcattcatat tgatcatatt atccttgttt
    10081 ttaaccgttg aagcattcaa gcggttcact tcaccagaaa agataaatag tcatttgatg
    10141 ctaacagtag cagttattgg attgctcgcg aatttgttct cagtattatt attacgacaa
    10201 gaagcagatg aaagtttgaa tataaaatct agttatttgc atttgttaag tgatacactg
    10261 tcttcggtaa gtgtgattat cggagcaatt ttgatccgtt ttttcgatat ctactggatc
    10321 gatccagtta ttacactgat catttcgata tatatattga tagaagcgat aatggtgatc
    10381 aaaaaagctg ccgctatttt gatacaaagt gcacctgcta tcgactatga aaaaatggaa
    10441 caagaaattg aagcaatcga tggggtaaag gatgttcatc atgtccatat ttggcagtat
    10501 agcgaaaaga taattatttt cgacggacat attgattttg aagatcagct actaagcgag
    10561 attgaaaaga tatatcctcg tataatttct ttattaaaat taaaatatgg tattacacat
    10621 gtgacgatcc aagcagagac aaaggcaaag gatcaaaaaa aattaatttt tttaaataaa
    10681 gatgaaagtt attgaaatat agagtatgat aaataagaat ttttaataag gtaggttggc
    10741 tcatgaagca gtatcgacag ttaaaaggat atttagaagc aaaaggatat tgggagatac
    10801 tagtctatct cttttttggt ggtttggcta cgattgtaaa ttttgttagt tttgctctcg
    10861 cacgccaata ttttgattta agtatggcct tatccaattc tatttcgtgg ttttgttctg
    10921 tattgtttgc ttttgtaacg aacaaacttt gggtattcca ttcaaaatca ccgaatttta
    10981 cacatgcact tattgaatgc ggtaagttct tcttctaccg tatcctttct tacggattgg
    11041 acatgggggc aatggtattg ttgatcaatg ttatgaacag caatgagtat gtggcgaaaa
    11101 tcatcactca aattattgtt atccttgcga actatatttt cagcaagttg tttattttta
    11161 aagaaactga ggtttttgaa gaagaagtag gtcaaagtga aaaagacgac taggcataac
    11221 aattgaagaa tttccttctc tttgttactc tttgtaatgt agacattatt aagaaaacat
    11281 tgaggaggaa tttttttatg acttacacat taccagatct accttatgca tatgacgcat
    11341 tggaacctta tattgacgaa gaaacgatgc atctgcatca tgataagcat cacaatactt
    11401 atgtgacgaa tttaaattca gcaattgaga aatacccaga attaggcgaa aaaacaatag
    11461 aagaattatt atctgatatg gacgctattc caacagatat caagacagct gtacgtaaca
    11521 atggtggcgg acatgctaac cattcatttt tctgggaaat catggcacca aatgctggtg
    11581 gcgaacctac aggagaaata aaagaagcga ttaatgaagc ttttggtgat ttttcttctt
    11641 ttaaagaaga attcaaaaaa gcagccgctg gacgatttgg ttctggatgg gcttggcttg
    11701 taatggaaaa tggaaaatta gctattacct ctactgcaaa tcaagattct ccattgatgg
    11761 aaggaaagac accaattcta ggtttggacg tttgggaaca tgcttattat ttgaaatata
    11821 aaaatgtccg accagattat attgcagcat tctggaatgt tattaattgg gatgaagtaa
    11881 acaaacgttt cgaagcagca aaataaccat ttcctccgtc atatcagtct taaaaataat
    11941 ttggaaaata aaagagcgaa agcttgctag cgttttctaa attgggtata atgttcttgc
    12001 aaggttactt gcaagaactt ttttggagga tatacattta tggaacacgg aacggttaaa
    12061 tggttcaaca atgaaaaagg atttgggttt attacagtag acggcggaga tgacgtattt
    12121 gttcatttct cagcaattca aggagacggc ttcaagagcc tagaagaagg tcaagaagta
    12181 gaattcacga ttgttgaagg tgcacgcgga cctcaagctg ctgaagtgac taaattataa
    12241 taatttgttg aataaagaca cgacttctta gagagtcgtg tctttttgtc tatttatgaa
    12301 gtttttagat tttttggcta agtaaatgca tttgttcact tgacatgtcc taactaatta
    12361 tcaagcaagg aacattttct tcgttagtaa tggattttaa agaaatttca gtttttggat
    12421 gttgaataaa agaccatttg tgaaaccact agtatacata ctcattacca tgtcaattct
    12481 attactttaa aagaaaatcg ttttactttg aagaggagca tatctatcca ttaaaagatg
    12541 atggtaatgc aatcactagt tcgagtcatt ttaagagcca tgcagtgagc tggaaataaa
    12601 acgaatagaa gatgggttta acaaaaggac cagtcatagg aagaaattgt cgtgaaatcg
    12661 aatgataatc tgaacggtag gcaaagactg ttaaaaagat gggaaaggta tgtgcgatca
    12721 ttttactgca cgattatttg gaaaatacct tgttgttcgc tttttctgga aaaaatacat
    12781 aaaaaaagat aaaacgattg cgaactgcgt gtattcggtc tttcgatctt tttcacggta
    12841 tggtatgata gttaagaatt ttgagaaatt ttaaaaaata ataatcgtta cacaaaaatg
    12901 ttttttaaag ttttcttatg attaaaaagt gaatttgctg attgaacagt tatatgagac
    12961 aatattttag aaaatgtgaa acagaggtgt attatgaagc gatggcaaaa agttctatta
    13021 accttgttag gggtattcgt tttgctgatt gccggattat cagcatacgg aatcagattt
    13081 atgggagaag cgagccaaac agtaaacaaa atttctaaaa actctaatcg aatatcctcc
    13141 aaacgttctg agagagtaag cattgatgac aaagaacctt tttcggtttt gctattaggt
    13201 ttagataccg gtggattagg aagaacggaa caaggacgtt ctgatacgat gatggtagta
    13261 acagttaatc cgaaacaaaa aaaatcaacg atcatcagtt tggaccgaga tatttatacg
    13321 aatatcgtag gttatggaac ggtagataaa ttaaatcatg cctacgcatt tggtggtgtc
    13381 gaaatggcca tggattcaat tgagcaactg ctcgatattc ctattgatca ttatgtaacg
    13441 atcaatttgg atggcatgga agatttgatc aatgcggtcg gtggcatcaa agtgaataat
    13501 aaaattgact ttactttgga tggtgtccat gtaccagtag gtgaacaaac attgaatgga
    13561 gaaaaaggtt tagcctattc tcgaatgcga cacgaagatc cagaaggcga tatcggtcga
    13621 caacgtcgtc agcgggaagt agtgacgaaa atcgtcaata aagttttaag tttagatggc
    13681 gtaagcaatt atagaaaaat tttaaaagca gttgagaaca atgtgacgac ggacttagat
    13741 tgggatgaca tgcttgatgt agcgacgaac tacaccccgg cgtttgaaac aatcaaacaa
    13801 gaccagttgc aaggcgaagg acaaatgatc aatgatatct actatcaaat tttaggaaaa
    13861 aatgatcttc tttcgatcca aaatgaactg aagaaacaat taaatataaa aacaagcgat
    13921 acattaccta atttgaaaaa tgagaatgct tcaatgatgt tctttgatga ttcggaagat
    13981 ggcgattcga caaacggtga tggatcgaat agtgaagggt acaccgatta ttcttcctat
    14041 tcagaaaaca catatgatca gtaccaagat ccgaatacct attatcagca aacagaagat
    14101 acaagtcagt actatacacc aagtcaagat actcaaagtt atgatcctta cgctggacag
    14161 gaacaaaatg gttatggtta tgaccagaat aatgagtatg gaacaacaag tgggtactag
    14221 aaaaaaatag tttggttaat aaagagtgcc acctaaatta gacaaaaaa
//
LOCUS       DAZBWR010000072        14222 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000072 DAZBWR010000000
VERSION     DAZBWR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.074"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHGT5_001880"
     CDS             complement(<1..1835)
                     /locus_tag="ACHGT5_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN7217858.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHGT5_001881"
     CDS             complement(1832..2536)
                     /locus_tag="ACHGT5_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN7217859.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHGT5_001882"
     CDS             complement(2548..4857)
                     /locus_tag="ACHGT5_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN7217860.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHGT5_001883"
     CDS             complement(5052..5516)
                     /locus_tag="ACHGT5_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217861.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHGT5_001884"
     CDS             complement(5516..6142)
                     /locus_tag="ACHGT5_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN7217862.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHGT5_001885"
     CDS             complement(6149..6514)
                     /locus_tag="ACHGT5_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN7217863.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHGT5_001886"
     CDS             complement(6504..6842)
                     /locus_tag="ACHGT5_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217864.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHGT5_001887"
     CDS             complement(6832..7158)
                     /locus_tag="ACHGT5_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN7217865.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHGT5_001888"
     CDS             complement(7139..7417)
                     /locus_tag="ACHGT5_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN7217866.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHGT5_001889"
     CDS             complement(7419..8783)
                     /locus_tag="ACHGT5_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN7217867.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHGT5_001890"
     CDS             complement(8796..9365)
                     /locus_tag="ACHGT5_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFN7217868.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHGT5_001891"
     CDS             complement(9337..10524)
                     /locus_tag="ACHGT5_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN7217869.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHGT5_001892"
     CDS             complement(10588..12315)
                     /locus_tag="ACHGT5_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN7217870.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHGT5_001893"
     CDS             complement(12312..12764)
                     /locus_tag="ACHGT5_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN7217871.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHGT5_001894"
     CDS             complement(12876..13256)
                     /locus_tag="ACHGT5_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN7217872.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHGT5_001895"
     CDS             complement(13253..13639)
                     /locus_tag="ACHGT5_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFN7217873.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHGT5_001896"
     CDS             complement(13641..13919)
                     /locus_tag="ACHGT5_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217874.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14222
                     /locus_tag="ACHGT5_001897"
     CDS             13982..>14222
                     /locus_tag="ACHGT5_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217875.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGY"
BASE COUNT         3938 a         3057 c         2239 g         4988 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 tt
//
LOCUS       DAZBWR010000073        14189 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000073 DAZBWR010000000
VERSION     DAZBWR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.075"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACHGT5_001898"
     CDS             <1..483
                     /locus_tag="ACHGT5_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFN7217876.1"
                     /translation="FGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIET
                     ALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNG
                     ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI
                     "
     gene            805..975
                     /locus_tag="ACHGT5_001899"
     CDS             805..975
                     /locus_tag="ACHGT5_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217877.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            989..1591
                     /locus_tag="ACHGT5_001900"
     CDS             989..1591
                     /locus_tag="ACHGT5_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7217878.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1607..2284
                     /locus_tag="ACHGT5_001901"
                     /pseudo
     CDS             1607..2284
                     /locus_tag="ACHGT5_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2595..3092
                     /locus_tag="ACHGT5_001902"
     CDS             2595..3092
                     /locus_tag="ACHGT5_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFN7217879.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3093..3509
                     /locus_tag="ACHGT5_001903"
     CDS             3093..3509
                     /locus_tag="ACHGT5_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFN7217880.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3529..5073
                     /locus_tag="ACHGT5_001904"
     CDS             3529..5073
                     /locus_tag="ACHGT5_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7217881.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5216..5440
                     /locus_tag="ACHGT5_001905"
     CDS             5216..5440
                     /locus_tag="ACHGT5_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217882.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5455..6324
                     /locus_tag="ACHGT5_001906"
     CDS             5455..6324
                     /locus_tag="ACHGT5_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFN7217883.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6305..7039
                     /locus_tag="ACHGT5_001907"
     CDS             6305..7039
                     /locus_tag="ACHGT5_001907"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7217884.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7072..7980
                     /locus_tag="ACHGT5_001908"
     CDS             7072..7980
                     /locus_tag="ACHGT5_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFN7217885.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7977..8519
                     /gene="sat4"
                     /locus_tag="ACHGT5_001909"
     CDS             7977..8519
                     /gene="sat4"
                     /locus_tag="ACHGT5_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFN7217886.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8612..9406
                     /locus_tag="ACHGT5_001910"
                     /note="aph(3')-IIIa"
     CDS             8612..9406
                     /locus_tag="ACHGT5_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN7217887.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9682..9813
                     /locus_tag="ACHGT5_001911"
                     /pseudo
     CDS             9682..9813
                     /locus_tag="ACHGT5_001911"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9889..10382
                     /locus_tag="ACHGT5_001912"
                     /pseudo
     CDS             9889..10382
                     /locus_tag="ACHGT5_001912"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10552..11448
                     /locus_tag="ACHGT5_001913"
     CDS             10552..11448
                     /locus_tag="ACHGT5_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7217888.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11547..11756
                     /locus_tag="ACHGT5_001914"
     CDS             11547..11756
                     /locus_tag="ACHGT5_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN7217889.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11773..12045
                     /locus_tag="ACHGT5_001915"
     CDS             11773..12045
                     /locus_tag="ACHGT5_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN7217890.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12047..12910
                     /locus_tag="ACHGT5_001916"
     CDS             12047..12910
                     /locus_tag="ACHGT5_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN7217891.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACHGT5_001917"
     CDS             complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACHGT5_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN7217892.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14035..14118)
                     /locus_tag="ACHGT5_001918"
     CDS             complement(14035..14118)
                     /locus_tag="ACHGT5_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN7217893.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5018 a         2222 c         2928 g         4021 t
ORIGIN      
        1 tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact
       61 ttagttgaaa gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta
      121 gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct
      181 attacgtttg actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat
      241 attgatatat attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct
      301 aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg attttagaaa agtgaatcag
      361 acatttattt ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac
      421 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
      481 tgacaaatca tactatgata cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag
      541 aaatatagag agtaagattt taattaatta ttagggggag aaggagagag tagcccgaaa
      601 acttttagtt ggcttggact gaacgaagtg agggaaaggc tactaaaacg tcgaggggca
      661 gtgagagcga agcgaacact tgatctttta agttgctatc tttttaaggt caatagagta
      721 tacttatttg tcctaatatg gttttagcag tataattgac ttggtgaata ggtcatttaa
      781 gttgggcata ataggaggag taaaatgaaa aaatttattt atcgagtttt agaaaatgac
      841 gaagtggtgg ctatttttaa tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag
      901 acaatttctg ataagcaatt tgaaattgaa aaagtagata tagctgattg gttattgcaa
      961 ccgagagaat tttagaggtt ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag
     1021 caaagaacag aacttagata gacaactgaa agcgttagag ggcgtttcta aggtcttttc
     1081 agacaaatta agcggtcaat cggtcgaacg cccacaatta caagctatgc ttaactatat
     1141 tcgtgaaggg gatatagttg ttgttactga attagatcga ttaggacgaa ataataaaga
     1201 attaacagaa ttgatgaatc aaattcaaat taagggggca accctggaag tcttaaattt
     1261 accctcaatg aatggtattg aagatgaaaa tttaagacgg ctgattaata atttagtgat
     1321 tgaattgtat aagtaccaag cggaatctga acgcaaacga attaaagaac gccaagccca
     1381 aggaattgaa attgctaaga aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga
     1441 aaatgatcca cgtttacaac acgctttcga tttatttttg aatggttgtt ccgataaaga
     1501 agttgaagaa caaactggaa ttaatcgccg aacgtttaga aggtatcgat caagatacaa
     1561 cgtgacagta gatcaaagaa aaaaacaatg aaaagaggga tagttaatga gtacggttat
     1621 tttagctgaa aaaccaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
     1681 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
     1741 tggttttggg catttagtcg aattagcaga accaggtcat tataacgaaa agtggcagaa
     1801 ctggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     1861 taagggaaag cagtttaaaa ttgttgctga acttctcaaa aaggcaaata caattattgt
     1921 cgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
     1981 tgccttttct aaagataaaa cgtataaaag attatggatc aatagcttag aaaaagatgt
     2041 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     2101 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac
     2161 cttgaattta caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc
     2221 gactttatat ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt
     2281 tttcctgaat aattcatagt tttactaata gtctttccta acattgattt aatagtcttt
     2341 aaagtattct gcttcatcac ccattaggtg atgaaaaaag aacccctttc tgatttagcg
     2401 gacaaatact ttgacctaat ctcagaagat ataaaagaaa aaataataaa atatccgatt
     2461 tatactttgt ataaagaact aaagtagtta gcaaatcatt tacagcaaat cactatgttc
     2521 cacataccat tttacatact taccttatcc acgacacatg ttgagatagt atcgaagata
     2581 aagaaagttg ctccatgttg tttttgcgag atggggaaat caaagccaca ttgacaacca
     2641 tgatgaataa gcttgctttc agtcataagc taatcctaga accacttttc aagtcagtta
     2701 gccaaatcga tgaagaaagc gaccgtgaaa gaatggacgc tattgataag ctaatggagc
     2761 aactattgga agaacgcaat acccttattg cactgatgtc caaaggtttc ctagaacctg
     2821 ctctttttaa tcaggaacga aatgttttag atagtgagat taaaaatctt actaccgaaa
     2881 aaacaaacct ggtaacgaac tctgcgagtg gggttttgcg agcaaacgag ataaaggacc
     2941 tcattaatta tgtgtcagca gataatttta atggtgacta cacggaagaa ctatttgaag
     3001 aatttgtagt gaacatcatt gtaaattcca gggatgagct gacattcaat ttgaaatgcg
     3061 gtctttccct gaaagaaaag gtggtgagat aaatggcata tattccatat ggatacaaaa
     3121 ttcaagatgg agtggttact gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg
     3181 agaaatacat atcaggacta tcccttacag tggctggcga acaggcaggt attgaaaaga
     3241 cacattctgt gatggggcgc attttgaaaa acgtcaacta ccttggaaat gatacgtatc
     3301 cagcaattat tgataaagag atatttgata aagctgaaga agttagagat aaacgtgcaa
     3361 aggatttagg acgagtggta gagcttgccg ctttcacctc tccccctccc aaagaacgat
     3421 tcaaaatgaa aaaggcagat aataagatgc cagtggatcc ttttgaacga gcagaatact
     3481 tatacagtct gatagaaagc gaggaataaa gtgacagaga aaaatataat ggttattcct
     3541 gctcgtaaaa gagtaggaag tacagccgca aaagaaaaga taaagaaact tcgtgttgct
     3601 gcctattgcc gtgtttctac agaaacagaa gaacaaaatt ctagctatga ggttcaggtc
     3661 tcacactata cagagtttat aaagaaaaat aatgaatggg agtttgctgg catctttgca
     3721 gatgatggta tctctggtac gaacactaaa aagcgtgacg aatttaatcg catgattgca
     3781 gagtgtatgg acggtaacat cgacatggtt attacaaaat ccatcagccg atttgcgcgt
     3841 aacaccctag actgccttca atacattaga cagctcaagg ataagaacat atcagtttat
     3901 tttgagaaag agaacatcaa caccacggat gccaaaggtg aggttttgct gactattatg
     3961 gcatctttgg cacagcagga aagccagagc ctttcacaaa acgttaagct tgggctacag
     4021 taccgatacc aacaaggaaa ggtgcaggtc aaccataagc gatttatggg ctacacgaaa
     4081 gatgaagatg gaaatttaac tattgtcccc gaagaagctg agattatcaa acgcatctac
     4141 cgagaatacc ttgagggtca gagtctagtg ggcattggtc gagcccttga aaaggatggt
     4201 attttaacag cagcgggaaa accaagatgg cgaccagaat cagttaagaa gatacttcaa
     4261 aacgaaaaat acatcggaga tgcccttctg caaaagactg tcacagtaga ttttctgacc
     4321 aagaaacgag tgaaaaatga agggcatgtt ccccaatatt atgttgaaaa tagccatgaa
     4381 gcgattattc ctaaagagtt atttttgcag gcgcaagaag aaattcatcg gagaagcaat
     4441 atctacacag gagaaggcaa gaacaaaaga atttacagta gcaagtacgc tttaagtgcc
     4501 atcaccttct gtggagattg tggcgatatt tatagacggg tctattggaa tatccacgga
     4561 agaaaagaat ttgtctggcg atgcgtgact agaatcgagc aaggtcctga agtttgtaag
     4621 aaccgaaccg taaaagaaga tgaactttat ggtgcagtaa tgactgcgat taataagcta
     4681 cttgcaggtg ggaacaatat gataaagaca ctggaagaga atattcatgc tgtgattggc
     4741 gaaaccacag aataccaaat ttcagagatt aacaactcac tagaggaaaa acaaaaagaa
     4801 ctaatcaagc tggcgaacaa gggtcaagac tatgaccact tagcagatga gattgatgag
     4861 ctgagagaca agcgacagat ccttttagta gaagatgcct ccctcagtgg cgagaacgag
     4921 cgaatcaatg agctgattga atttatccgc aagaacaaat tccgcacctt agaatatgat
     4981 gataagcttg taaggaagat aatccagagc gttacagtct atgaaggcca cttcgtcata
     5041 gccttcaaat ctggcatcga aatggaaata tgaaaaccag aagtaaatag cccatgactc
     5101 tacagtagag ttgtgggttt tcttttgctt gctgattata ataatatttt aataactatt
     5161 gtgtttatca accaaatggt ttataatatt cttagcgtaa acttggaggt gctgtatgac
     5221 tacggcagaa atgattaaag aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc
     5281 tagacgtatt ggccagactc cacagaattt caataaaaaa ttacaacgag aaacagtaac
     5341 cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat
     5401 tttaccagat ggagaagaaa taaaaatatc taacgaataa aggaggatgt ttatatggta
     5461 ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt tgccttttat agaaggcatc
     5521 gtattagggg gctcacgtgc aagaggcacc catacagaaa attctgatat agatatcgga
     5581 atctattaca attcagactc atttgacctg acagcgatta atcaaattgc tacagagttg
     5641 gatgatgaga atagaaacaa ccttgttgta cctcccggag cgtggggtga ttgggttaat
     5701 ggcggtggat ggttagttat aaacgggtgt catgttgact tgatattacg tgatataaaa
     5761 cgggtggaac aaataatcaa agatacggag caaggaattg ttactgccaa ttatcagacc
     5821 gggcatcccc atggttatat aagtgctatg tatcgtggag aattagcgat tagtaaaata
     5881 ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact
     5941 gctctaaaga aaagcttgat gaactttttt atatttgaag ctgaattctc tttaatgttt
     6001 gtaaaagcaa atgcgggggc agaggataaa tattatattg caggccatgt ttttcgtatt
     6061 atttcatgct taaatcaagt actatttgca tgtaataatg cttattgcat taacgaaaag
     6121 aaagctataa aactgcttga aacttttgaa tataaacctg aaaaatatgc cgaaagagtt
     6181 aatcatattt ttgaagtact cggtctttct ctttttgaat gctacgacat gaccgagaaa
     6241 ctttataaag aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     6301 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     6361 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     6421 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     6481 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     6541 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     6601 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     6661 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     6721 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     6781 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     6841 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     6901 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     6961 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     7021 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     7081 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     7141 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     7201 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     7261 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     7321 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     7381 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     7441 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     7501 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     7561 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     7621 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     7681 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     7741 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     7801 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     7861 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     7921 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     7981 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     8041 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     8101 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     8161 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     8221 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     8281 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     8341 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     8401 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     8461 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     8521 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     8581 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     8641 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     8701 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     8761 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     8821 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     8881 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     8941 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     9001 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     9061 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     9121 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     9181 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     9241 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     9301 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     9361 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     9421 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     9481 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     9541 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     9601 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     9661 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     9721 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
     9781 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
     9841 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
     9901 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
     9961 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
    10021 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
    10081 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
    10141 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagcct
    10201 atatcactgg ggttttatga acagaactgg tacttaatcg gttttgcagg ctaagaaatg
    10261 aatataggaa ttttcgtgtg gatcggatta aaagcatttg catagaagaa gagctctgtc
    10321 aaagccatga tggctctttg gaacaaatat taaaacaaat gctttcatat aaaaaactat
    10381 atgccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc
    10441 tcaaaataaa tataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta
    10501 tcacaaatca caagtgatta atcacaaatc acttgtgatt tgtgattctt aatgatacaa
    10561 tattactata caaaaaaaga atggggcgta gttatggaga aggaaaaact aaaaatactt
    10621 gaagaattaa gacgtatttt aaacaataaa aatgaagcaa ttattatctt gaataattac
    10681 tttaaaggtg gtgttggaaa gtccaaatta tcgactatgt ttgcttactt gacagacaaa
    10741 ttgaatttaa aagttttaat gatcgataag gacttacagg caacattgac aaaagactta
    10801 gcaaaaactt ttgaggtaga attgccacgt gtcaattttt atgaaggctt gaaaaatgga
    10861 aacttggctt cttctattat tcatttgact gataatttag acttgatccc tggcacgttt
    10921 gatttgatgt tactgccaaa attaactcgc tcatggacgt ttgaaaatga aagtagattg
    10981 cttgctactc ttttagcacc tttaaaaagt gactatgatc ttattattat tgatactgta
    11041 ccaacgccaa gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct
    11101 ttacaagcag aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat
    11161 ttgcaagaac agtttaaccc tggactagat atgattggtt ttgttcctta tttagttgat
    11221 acggacagcg caacgataaa atcaaacctg gaagaactgt acaaagaaca taaagaggat
    11281 aatttggttt tccgaaatat tatcaagcga agtaataaag taagtacttg gtctaaaaat
    11341 ggcattacag aacacaaagg ctatgacaaa aaagttttgt ctatgtatga gaacgtattt
    11401 tttgaaatga ttgagcgaat cattcaatta gaaaatgaaa aagaatagaa tcacaaatca
    11461 caagtgatta atcacaaatc acttgtgatt tgtgataggt gatgataaaa taaatagtaa
    11521 gaagaaatag aaagaagtga gcgatcgtgg gaaatttagg cgcacagaaa gcaaaacgaa
    11581 atgatacgcc aatcagcgca aaaaaagata taatggggga taagacggtt cgtgttcgtg
    11641 ccgacttgca ccatatcata aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag
    11701 ttatggatca agccttagaa gaatatatac ggaaatattt acctgacaaa ctttaaaagg
    11761 agtgaaaatg aaatggcagt tacgtatgaa aaaacatttg aaattgaaat cattaatgag
    11821 ttatcggcaa gtgtttataa tcgggtatta aattatgttt tgaatcatga actagatact
    11881 aaaaatactc gtttactaga agtgaatctt ttaaatcaat tagaagtggc acaagaagtt
    11941 gatttatttc aacaaccatt tgaagaatta caagctattc atgagtattg gcggtcaatg
    12001 aatcaatatt caaaacaaat tttgaataaa gagaaagtgg cttaacatgg cgaatattac
    12061 tgacttcacc gaaaagcaat ttgaagatcg tttagaaaag aatgttgaac gactaactaa
    12121 aaatagacta gcggttgaat cgccaaccgc ttttttactt ggtgggcaac cagggtcagg
    12181 gaaaaccagt ttgcgatcag caatttttga agaaacacaa gggaatgtta ttgtcattga
    12241 taatgatacc tttaaacaac agcacccaaa ttttgacgaa ttagtgaaac tctatgaaaa
    12301 agacgtagta aaacacgtta ccccttattc gaatcgcatg acagaagcac ttattagccg
    12361 tttaagcgat caaggctata atttagtgat tgaaggaaca ggacgaacaa cagacgttcc
    12421 cattcaaaca gccacaatgc ttcaatctaa aggttatgaa acaaaaatat acgtcatggc
    12481 agtaccgaaa attgaatcat acttaggaac gattgaacga tatgaaacca tgtatgcaga
    12541 tgatccaatg acagctaggg caacaccaaa acaagcgcat gatattgttg tcaaaaactt
    12601 accgaccaat ttagaaaccc ttcataaaac gggcttattt agcgatataa ggctttacaa
    12661 tagagaagga gtgaaactct attcaagctt agaaacgcct tccattagtc cgaaagagac
    12721 cttagaaaga gaattaaatc gtaaagtatc agggaaagaa attcaaccga ctttggagag
    12781 aatagagcaa aaaatggttc aaaatcaaca ccaagaaacc cctgaattta aagcaattca
    12841 acaaaaaatg gaaagcttgc agctacctac accaccaata cccaaaacac ctaaacttcc
    12901 aggtatttaa accttaaaaa aggaaaagag tagttaccaa aaaacggtaa ctactctttt
    12961 tttataaaaa catttcctca tgttggggtc ccgagcgcct acgaggaatt tgtatcgata
    13021 agaaatagat ttaaaaattt cgctgttttt ttctaatttc attttgaggt gaattaattg
    13081 gtagttatta gaggtgccct atacctaata atttatctac attcccttta gtaacgtgta
    13141 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
    13201 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
    13261 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
    13321 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
    13381 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
    13441 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
    13501 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
    13561 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat attcccaaca
    13621 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
    13681 acacgagtgt tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
    13741 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
    13801 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
    13861 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
    13921 ctgaagtgat tacatctata aataaataca gaagttaaac gatttgtttg taattttagt
    13981 tatctgttta aaaagtcata agattagtca ctggtaggaa ttaatctaac gtatttattt
    14041 atctgcgtaa tcactgtttt tagtctgttt caaaacggta gatgttttat ctacattacg
    14101 catttggaat accaacatga cgaatccctc cttcttaatt acaaattttt agcatctaat
    14161 ttaacttcaa ttcctattat acaaaattt
//
LOCUS       DAZBWR010000074        14068 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000074 DAZBWR010000000
VERSION     DAZBWR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.076"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      105..271
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            419..976
                     /locus_tag="ACHGT5_001919"
                     /pseudo
     CDS             419..976
                     /locus_tag="ACHGT5_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1034..3073
                     /locus_tag="ACHGT5_001920"
     CDS             1034..3073
                     /locus_tag="ACHGT5_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN7217894.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3182..4333)
                     /locus_tag="ACHGT5_001921"
     CDS             complement(3182..4333)
                     /locus_tag="ACHGT5_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN7217895.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4487..5017)
                     /locus_tag="ACHGT5_001922"
     CDS             complement(4487..5017)
                     /locus_tag="ACHGT5_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN7217896.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5116..5466)
                     /locus_tag="ACHGT5_001923"
     CDS             complement(5116..5466)
                     /locus_tag="ACHGT5_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN7217897.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5623..7152)
                     /gene="ahpF"
                     /locus_tag="ACHGT5_001924"
     CDS             complement(5623..7152)
                     /gene="ahpF"
                     /locus_tag="ACHGT5_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN7217898.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7162..7725)
                     /gene="ahpC"
                     /locus_tag="ACHGT5_001925"
     CDS             complement(7162..7725)
                     /gene="ahpC"
                     /locus_tag="ACHGT5_001925"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN7217899.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7869..8531)
                     /locus_tag="ACHGT5_001926"
     CDS             complement(7869..8531)
                     /locus_tag="ACHGT5_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7217900.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8715..10361
                     /locus_tag="ACHGT5_001927"
     CDS             8715..10361
                     /locus_tag="ACHGT5_001927"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN7217901.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10352..11434
                     /gene="uxuA"
                     /locus_tag="ACHGT5_001928"
     CDS             10352..11434
                     /gene="uxuA"
                     /locus_tag="ACHGT5_001928"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN7217902.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11542..13095)
                     /locus_tag="ACHGT5_001929"
     CDS             complement(11542..13095)
                     /locus_tag="ACHGT5_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217903.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13243..>14068
                     /locus_tag="ACHGT5_001930"
     CDS             13243..>14068
                     /locus_tag="ACHGT5_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFN7217904.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVT"
BASE COUNT         4765 a         2598 c         2605 g         4100 t
ORIGIN      
        1 ttttctaaaa aatattaaag attgttaaat ttcactattt catttcaaat tccattgcct
       61 aatcattagg aaaagtctat catgaactcg taaatagaat atagtaataa ccttgttagg
      121 tgaggctcct atacaaacat aggctgttgc ccagaaacgt cgaaagtcgc caatgggtaa
      181 aacagaaact gttggattaa ggctttttcc aatagctaag aatgttttct ttacgttgta
      241 tagtgctaaa actcggacga tgagatgatg ctaggtcaga attcttgttc gaaaaggttt
      301 ctgacctttt ctttatattt ttgtgactga cttatttgat gagacaaact cgctcctcac
      361 ctgacaagtg gattgctgtg tgaggaggag atgcggtgaa aaaagatcga ttggtggagg
      421 tcgctcggat tcacttctgg ttgcacaata caaccagaaa acacaacaag caaaactgat
      481 ctctatcatg agagattcat atgtcgatat tccaggttac ggaatgaata agataaatgc
      541 agcttactct tatgggggag ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt
      601 tgaagctccc tattatgcaa gtatcacatt tcaagatttt atcgattgcg ttaatgaact
      661 gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta gatttagatg gcgtatatat
      721 aaacaaagga gagcaagtga tggatggtaa tactttactc cagtatgctc gatttcggga
      781 agacaaagaa ggagacttcg ggaggatcag aaggcagcag caagtgatca aagctatctc
      841 tcagcagctg aaagacgtaa cttcaatatt caagctaccc aaagcggttg ggaaattact
      901 tggtagtatc caaacgaacc ttccagaaag tgtgcttctg gattgtggta tggatttcct
      961 caaagacata tgttagaata aacaggtata aatagtgaaa taaaggaatg acaagcaaga
     1021 gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat
     1081 tgccggcgca gtgatcttaa tagcagctgc gggtggcggt tatttttact accagcacta
     1141 tcaagaaacc caagcagtag aagctggaga aaagacagtt gagcaatttg tccaagcttt
     1201 gaacaaggga gattataaca aagctgcaga aatgacatca aaaaaggcgg caaataaaag
     1261 tgcattatct gaaaaagaga tcttagataa ataccagaat atatacggtg ctgctgatgt
     1321 caaaggactt cagatatcga atctaaaagt agataaaaaa gacgattcta cttacagttt
     1381 ttcatataaa gcaaagatga atacctcatt aggtgaattg aaagatcttt cttataaagg
     1441 aacattagac agaaatgatg gtcaaaccac gatcaactgg caacccaact tggtttttcc
     1501 agaaatggaa ggaaatgaca aagtaagcct gaccacacaa gaagcagcaa gagggaatat
     1561 tatagatcga aatggtgaac cattagcaac aacaggcaaa ctaaaacaat taggagtcgt
     1621 tccaagtaaa cttggagatg gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc
     1681 cttcgactta acagaagatg ctatcaatca ggcgatttca caaagctggg tacaacccga
     1741 ttactttgtc ccattgaaaa tcattgatgg agcaacgcca gaacttccag ctggagctac
     1801 cattcaagaa gtagacggca gatattatcc tttgggtgaa gcagctgctc agctgattgg
     1861 ttacgtggga gatatcacag cagaagatat tgataagaat ccagaattaa gcagtaatgg
     1921 aaaaatcggt cgctcaggtt tggagatggc ttttgataag gatcttcgtg ggacaacagg
     1981 tgggaaatta agcattacag atgcagacgg tgtagagaaa aaagttctga tcgaacatga
     2041 agttcaaaat ggcaaagata tcaaattgac aatcgatgcg aaggcacaaa aaatagcttt
     2101 tgacagtcta ggaggaaaag ctgcatcaac tgttgcgaca acgccaaaaa ccggtgatct
     2161 tcttgcgctt gctagctctc caagctatga tccaaacaaa atgacaaacg ggatctcaca
     2221 agaagattac aaagcttatg aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac
     2281 aggttatgct cctggatcta cgtttaaaat gattacagca gcaatcggtc tcgacaacgg
     2341 cactatcgat ccaaatgaag tgttgacgat caacgggctt aaatggcaaa aagacagttc
     2401 ttggggatct tttaaaacaa cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac
     2461 tgctttgatc tattccgata atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa
     2521 aaaatttcgt acaggcttag ataaattcat ttttggtgaa gaccttgatt tgccaattag
     2581 tatgaatcca gcacaaattt ctaatgaaga tagctttaac tcagatatct tgctagctga
     2641 tactggatat ggacagggcg aacttctaat tactcctatc cagcaagcag caatgtattc
     2701 tgtttttacc aacaatggca cacttgtcta tcctaaattg attgcagata aagagacaaa
     2761 agataagaag aatgtcatca gcgaaacagc agtacaaacg atcgtgccag atctgagaga
     2821 agttgtgcaa gatgtaaatg gtacagcaca ttctctttct gctttaggga ttccgttggc
     2881 agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat gtaaaggggc aagagaacag
     2941 tttcttgttt gctttcaatc ttgataacca aggttatatg atggtcagca tgttggaaaa
     3001 taaagaagat gatgattcag caaccaaacg agcatcggaa ctattgcaat acctcaacca
     3061 aaattatcaa taaaaaattg ctttttaagt acagtaaaag gactgtggcg ttatcgtcac
     3121 agtccttttt tcaaataaac ggttttcacg atgtctcttt cacaacttct ttttgattta
     3181 ctcacacttc ataaactttt tttgtaaaat atttgagaac aagtggagaa ataagtgtac
     3241 ttaataaaat cacgataact aaaggtgaat agtactgtgg ttcgatcaat tgactttgct
     3301 gtccaatttg taaaatgatc aaagccattt ctccacgcga aatcatacca gcaccgacca
     3361 tcaatgaact atgttgagaa aaaccggcaa tctgagcacc aaaataacct ccaagcaatt
     3421 tggtcaaaat cgctacaagc gtcaatatag aaataaagag tagttgttca ctaaagtcag
     3481 aaaaatcaac ttctaatcct acactgacaa aaaatactgg tataaatact gcatatccta
     3541 aagcctctac attgtggtat acttcttctt taacctttgt ctgactgaca gctatcccag
     3601 caaaaaaagc accaatgaca gagctaagcc caacaagatc cgctacgtaa gacattccta
     3661 aacaaataac tagcgatacg atgataatag aagaatttgc gtacactttt tctgcaaatg
     3721 aaagtaggaa aggcgcaatc catttgacta ataagaaaat aaaaacaaaa taaattagct
     3781 gttcaatcaa aacaagcggt aaagaagcat tgctagaagc ttcccccgtt agaaaagata
     3841 ggctgaaact tagaactaaa acgactaaaa tgtcatctac cacagaagca cccaaaattg
     3901 ttgatccttc ttttgtattc actacgttta gttcttttaa tacttctact gaaatactga
     3961 cagaagttgc cgcaagaata atcccaaaga aaatcgcttc atttcccgaa acttggaatg
     4021 ctttgcctgt caaccagcca aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca
     4081 taccaggtcg gaaatatttt ttcaaaagag ataaatcact ttccagacca gctaaaaaca
     4141 ttaataagat cacaccaatc tccgaaaagt catggactag aatatcaggt ttgacccaat
     4201 tcaatccagc ttggcctagc aaaacaccaa caagtaattg tccaatcact gctgggatac
     4261 ctaatctccg cgagatatgt gagccaatcg ttgttgaaat caatatcagg cataaaatac
     4321 caataaattc cattctcatc attcctttgt ataaaatata gcccgaactt agcataacat
     4381 acattattga aaaaatagga aaatatactc acaaaaaaac caacaatcca aaagattttt
     4441 ttggattgtt ggtttttata cggtctattt taataactaa aaatgattaa cgtatagtca
     4501 tttttcttat tctgatgaaa ttgataatct cctggatgcc catgaaaatc aaaataaagc
     4561 cgaatatttg gaatagaagc atcaaacttt tgaatggatt gaacaataag acgaatccgc
     4621 caatcaacaa cagaatacta ctataaacac tccaactttt agcttttgta ttcatgctga
     4681 agaacaactg gaacaagctg ttgatgataa cagctaatcc aagaagaatc ggcaggatag
     4741 aaacgattgc aggtgcaaaa gcatagacga tcagagctaa gatcaggaaa aaaagaccac
     4801 tgaacagacc gatactccaa gaacctgttt tccttttaaa acggtaagcc tcaaaaagat
     4861 tcaacaaccc aagcaaaaca agataggcgg tgatcagata accgataaaa tgaaaaacag
     4921 cagaagggtt tagcacaatc gcaatcccag aaataatata aacagcagct cttaataagg
     4981 cgtatttttg aatattttgt ttaatcgtct caaacatata agaaaacctc ctttgactta
     5041 ttttaacgtg aactagtcat tttaccaaat taaatgttac aaaatgagcg aaggagaaga
     5101 atatctctgg aaaaatcaac ttctgaggat cttttccatt tttttgcctt ttgctagctc
     5161 atcgatcatt ttatctaaat aacgaatttc tttcatcaag ttatcctcga tgtcttctac
     5221 tcgtacacca caaacaacac ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc
     5281 cgcaaaaaat tgttcgaaag tcacttgttt ttgtagttga tattctagtt ctgtttggga
     5341 ataacccgtc agccaacaaa tgatttcatc tacttcctct tttatacgat cttttctctc
     5401 cgcttttgct acatacatag gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg
     5461 cgccataatc agtcaccttt ctttttttca aattatttac tacttatagt atataagaaa
     5521 aagagaagaa aaagcgatta ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac
     5581 catccttttt tccgctctct gactttttac aatcttcata gctcaagtgc gcatcagata
     5641 atcgaatgct cctaaagctg cagtagctcc tgaacccatt gaaacaatga tttgtttgta
     5701 tggactatct gtacaatcac cagctgcgaa aacgccgggg atattcgttg cgccattttt
     5761 ctcggtcact atttcatttc ttggattcat ttcgatcgtt ccatctagcc aatccgtatt
     5821 agggagtaag ccgactagaa tgaatactcc tgaaacagct agtgtatgca tctccatcgt
     5881 acggcgatcg atataggaga gagattctac ctgattcgta ccgctgattt ctttcgtcgc
     5941 tgcatgtttg ataacagtga cattcgcaag agagactagt ttatcctgta agatttgatc
     6001 tgcttttaat tctggtaaga attctagaac atatacatgt tttgctaacc cagccaaatc
     6061 gattgctgct tcaacaccag aattaccacc accgataaca gcaatctctt ttcctttaaa
     6121 taaagggccg tcacagtgtg ggcaataagc aattccttta tttttaaact ctttttcgcc
     6181 tggtacattg atagaacgcc agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag
     6241 tgatataccg ttagctaagc caatattaat aaaatgttgc tctttattta aggaaacggc
     6301 ctgttgattc atcataatgt cgatagggta actacgcaca tgactttcta cttgctgcat
     6361 caattttgga ccttctgtgt aagaggtacc aatcatattt tcaatcccta gtgtttcaac
     6421 gacttgtccg ccgaaagaat cagcgacaag tcctgtacgg attcctttac gtgctgcata
     6481 gatagctgca ctactagctg ctggtccacc gccaattact aatacatcat aaggttcttt
     6541 ttcaaacaac tcttcttcaa tcaaagggcc agtgattttt tcaacaattt gttcgatcgt
     6601 catgcgtccg ctactaaatt cttctccatt taaaaacaca gttggcactg ccatgatttt
     6661 tttatcatcg atttcttttt ggaacatccc accttcaatc atcgtatgag taatatttgg
     6721 atttaataca gctaatatat tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa
     6781 gctaacataa ctgtcaaaat gcaatgtttg tgttattttt ttgattttaa gactcaaaga
     6841 ttcactgatt ttcggtgccc gtccactaat ttgtaacata gccagtaaaa aagaggaaaa
     6901 ttcatgaccg agggggatac cagcaaaaac gattccactt tctttgccaa ctggattgat
     6961 agaaaaacta ggtgtgcgca tcgaatctct tttttcccaa gagattttag gagacatttc
     7021 tgctatttcc ttcgtaaatt cagctagttt ttgactattt tcactattat ccaaactaac
     7081 agaaaaaaca ataggagatt caatcagtgc taaatattgt tcgagatttt gtttagtttc
     7141 tttgtctaac atatccacca cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt
     7201 ttcgccactt tctttccatt ttgccggaca aacttcacct gggtgggtac gtacatattg
     7261 agcagcgcga atcttatcaa tcagtgcact agcgtcacga ccgattccat ctgcattgat
     7321 ttccatagct tgaacgattc cgtctggatc aataataaat gttcctcgct gagctaatcc
     7381 ttgctcttca tccaaaacat caaaaatacg agaaatctga tgagacggat caccgatcat
     7441 cacgtattcg attttaccga tagcatctga tgtatcatgc catgctttgt gagtaaagtg
     7501 ggtatcggta gagcaagaaa agacttcaac acctaaagat ttcaatgtac tgtattgttc
     7561 ttgtaagtct tctaattcag taggacaaac aaaagtaaaa tcagctggat agaagcaaag
     7621 aatactccag ttccccataa gatctttttc actgacttct aaaaattctc cttgatgata
     7681 agcatgtgca ttaaattctt cgattttttt cccaatcaat gacatagtaa aacctccaat
     7741 aataattatt tatttataat tattgtaatt tagttggagt tgcaaagtca atggatattt
     7801 tgttatttaa taaagattac tcaaaaacga aggttaatta taaaaataga agaggtcgca
     7861 agaaaatgct actgctggaa atactcggga taacgttgag taacagtttc ctgttctttc
     7921 agcatcatgt gtaagtgcaa tcccgtttga aatcgagctt cttctggttt tttatcaact
     7981 aaagccttga gtaaatcata atgttgttcc atgatcgttt cccatggaag cccctgaatg
     8041 gtcactcgta accagcggaa acgttctaaa tgggtattat ggtcatctaa ccagttccaa
     8101 acttcttttc ttcctgcaat ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa
     8161 aaagcgcgtt cgtcatttat tttgactgct ttttcttgtt cttctaaaat cgtttctagc
     8221 actattcgtg tttgtgtgtc caatttggaa caacattcaa tcatgatttg ctcttccaac
     8281 tgttctcgga caaagcgagc attttctgca gaaacaagat taattttgga aacatacgta
     8341 ccactttgag gtatcgtata cacgagttct tgctgacgta gttgaatcaa tgcttcgcga
     8401 atgggagtcc ggccgatagc aagatgttcc tccagtgcct tttcagatat tttccttcca
     8461 ggttctaagt cagcatagat aatttgttta cgaatcgctt gataagcttg tgattgcaag
     8521 ctgcttttca tctttattcc tccaaaatca tttctatgaa cgtagtataa cagaagaggt
     8581 ctgaaaaaca atctggtatg tcacattaac tagtaaattt gtataaatag taaaattttg
     8641 gttgacatag cgatttttat tgtataaaat tcacttgaat acaaaaacta atatgttagt
     8701 ttaggagggg aaaaatgatt gatttaaaca actggaggaa ctttatccct gaattatctc
     8761 aaaaagaagt agaaatacct cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa
     8821 atcctgtctg gatccatttc ggtggcggaa atctttatcg aggatttcat gcagaaatcg
     8881 ctcaacgttt agcggatcta ggcgaattgt ccactggtgt cattgtttgt gaaacattcg
     8941 atgaagaaat aattgaaagg gcttaccaag attttgatga tttgattctt gaagtggtca
     9001 tgccaagcag tggagagttc gtcaaacgta tattagctgc gacagctgac agtctgtatt
     9061 gtcatccaga ttgttctgaa cactttgata agatgattcg acactttgaa aatcctagtt
     9121 tacaattagt tacttttgct attactgaaa aaggttatcg attgaaagat acaaataata
     9181 aatatatagg accgattgca gtcgatataa aaaatggtcc aaagaagcca tcacatacaa
     9241 tgagtattct tgctgcgcta ctttataaac gttttttatc tggagcttgg ccgattgcac
     9301 ttgtctctac agataatttt tcacaaaacg gtagtcaatt acgtacttct ttgctaacga
     9361 tcgcaaaaga atggcaaaag gaaggttatg tacaaaatac atttatccaa tacttatcga
     9421 atgaagaaat tgttgcgttt ccatggacga tgattgatcg tatcaccccg aatccttcct
     9481 tgtcagttag caaaatgttg gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg
     9541 caaaaggaaa taatattgct ccatttgtca atacagagga catccattat ttaatcatgg
     9601 aagatcattt tcccaatgga cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc
     9661 gtgcaacggt agataaagca gatacgatga aggtgacgac ttgccttaat cctttgcata
     9721 cgtctttagc tattatgggt tgtttactgg ggtataggag catagctgag gagatgaaag
     9781 atccagattt atcgctgcta gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg
     9841 agtctccagg tatcattgat ccaaagatct ttctggaaga agtaattact gtgcgctttt
     9901 ctaatccaaa tattcctgat atgcctcagc gaatcgccac ggatacctct caaaaggtgg
     9961 cgattcgtta tggtgagacg attaaaaaat atatggaaat agaagttaaa gacccgcaga
    10021 cacttgtatt cataccattg actctcgctg cttggcttcg ataccttatt ggcattgatg
    10081 atcaaggaaa accattcatc ccgagtccag acccactatt gacagacttt cagcataagc
    10141 tttccgatat tcaactgggt acgcaagata catcacatat tcatgagaaa atggctccta
    10201 ttttgtcaaa taaagaaatt tttggtgtag atctttacca agcagagctt ggcagaaaaa
    10261 tagaagaaat gttcatcaag atggtagcag gaccgaatgc cgtacgaaaa acattacagg
    10321 attacttaca agaacatgga ggaaatttat catggaaatg acttttagat ggtacggatc
    10381 acaggaagaa aagattacct tagatgaaat cagacagatt cctgcggtca aagggattgt
    10441 aggcacattg atggatatac cagtaggaga ggtatggccc agggagagga tcaaagaact
    10501 aaaagaagaa attgagtctc gtgggttaac actaaaagta attgaaagcg tcaatgtgca
    10561 tgatgccatt aaaattggat taccttcccg tgagcagtat atcgaaaact ataagcagac
    10621 aatccgaaac ttggcagaat atggaataga agtcatctgc tataatttta tgcctgtatt
    10681 tgactgggtg aaatctgacc ttgactaccg cttagaagac ggctcatcca cgttagcttt
    10741 tatatctgat gatattccag atgatccaag agaaatcgtc gcgcgcatcg aacattccag
    10801 caatgaattt gaattgccgg gatgggaacc tgagcgctta gctcatatca aatccttgct
    10861 tgaagcctat gcatcagtag acgaagaaaa acttcgagaa aattttgcct attttctcca
    10921 atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc
    10981 gccatactcg atttttggat tacctcgaat cgtaaagaat cgagaagatt tagattggtt
    11041 gtgccgcata gtagacagcc cttataacgg aatcactctt tgtacaggga gtattgcaga
    11101 agatccagaa aatgatgtat atgggatttt agcagaattt tgtcaaagag atcgaatccc
    11161 atttgcacat gttcgaaata ttaaattttt agctaatcga gatttctatg aggcaccgca
    11221 taaatccgaa tacggttcat tagacatgta ccgcatacta aaaacaatgt atgacaacgg
    11281 atttaacgaa tacatccgtc ctgatcatgg aagaatgatc tggaatgaga caggaagacc
    11341 aggatacggc ttatatgatc gagcattagg tgctgtttat ctaaatggat tatgggaagc
    11401 aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa aaactgtaat tttattatgt
    11461 aaataagaag gatgttcaaa ttcttgatac ataaatccgg aggttgttaa taagcattcg
    11521 gatttatgta tcttatttgg tctaggaagt aacttgattt tcttttttcg attgatccat
    11581 gagtgcttct tcttttaatt caaagatata ttctgagatc ctcataatca cttttttcca
    11641 gtcttctggt gtgatgattc cttttaaaat atagattttt ttgatatgag aaggcaattg
    11701 tttcaaaggg cagtcgctga taacgatgtt gcagttctcc ataaaatcat tttgtatttt
    11761 gatcgattca tttgaaaaaa tttgttctat cttaacgatc agatctttac tgatataaaa
    11821 atcacgcgta tagttcacac caatcacaat tcgcggctgt tttttgtagc gatcatacaa
    11881 atgtactagg tcttcaacca tccattgcac attttccttt tctaaaataa ctggtaacaa
    11941 gtgttgattt tgttctctga aatccatata aaattgttca atggcggttt ggacttcttt
    12001 caacacgcta tgttcttgat aaagcatgaa agtaggaagg ggcgtatttg gttgattgaa
    12061 gttaagaaat ccattataaa gtttatttcg tgcgaatgcc aatgagtaaa gaatcttttc
    12121 tttttcaggt ataaacaaat caaatacagt acaaaaggac tccaccattt ctcttgaata
    12181 aagaacatga ggaacgtttg ctttttttaa aagtttgtat tgctccaagc ttgtttcttc
    12241 cgattcgatt ttcgtgatag aaagtcgagc taaaatattc actaagatgc gttgttcgcg
    12301 acaaattggc agattaggtc gcaaaatatc tacagaggga tcaataaaaa agtcaatata
    12361 ttcatactct gtgaattctt tttgtacgtc tttcacagaa gttgcaggaa aattctcttt
    12421 acatattttt aatagaaaat ataatttatc tatcataccg ctatttagtc ggtcaagtac
    12481 ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt catcataaaa
    12541 ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat attgaatttg
    12601 tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag acaaaccgta
    12661 caatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg cactatagtt
    12721 tgtttgctga cttaaactga taatcgaatt tgtctgacag ctgaacaaag aagtcaagat
    12781 ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc agtaagccgg
    12841 atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat gcagttctgt
    12901 tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa aatttgattc
    12961 agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat gttcagatag
    13021 gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagagaaacg ttttaatcgc
    13081 ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact tgaaaatatt
    13141 actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga atctctacta
    13201 tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata ttcaaccatc
    13261 ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac agtccacaca
    13321 gaggagatag aaacagccaa atggacagaa accaagtggg ccaacgtcaa tcgaattacc
    13381 tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa aggacaaaca
    13441 gcgaccatca aagcaaaaat tgactattca ggagacttag cacctatact acaatcagct
    13501 gttacatttg agaatgttga tcccgctttg tcgattggct atgacgagaa cactttgact
    13561 atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga cttgttaaaa
    13621 ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca tcatttaacg
    13681 ccgtatagtc agcggcagac aattgagcaa gattcagcta tcgactcagg aggagatctt
    13741 gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc gcctacagaa
    13801 aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga tacagaaact
    13861 caaccagagc cagacaatga atcaaaacct gaaataaccc ctggagaaaa accagatgta
    13921 gacccagaag aaaaaccaga tgtgactcca gaaccagata cggattctgg aaaccaaaca
    13981 gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta
    14041 gccccagaag aaaaaccaga tgtaactc
//
LOCUS       DAZBWR010000075        13959 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000075 DAZBWR010000000
VERSION     DAZBWR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.077"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACHGT5_001931"
     CDS             <1..135
                     /locus_tag="ACHGT5_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN7217905.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACHGT5_001932"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACHGT5_001932"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN7217906.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACHGT5_001933"
     CDS             complement(2412..2663)
                     /locus_tag="ACHGT5_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN7217907.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACHGT5_001934"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACHGT5_001934"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN7217908.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACHGT5_001935"
     CDS             3613..3831
                     /locus_tag="ACHGT5_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217909.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="ACHGT5_001936"
     CDS             complement(4120..4632)
                     /locus_tag="ACHGT5_001936"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN7217910.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACHGT5_001937"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACHGT5_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN7217911.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="ACHGT5_001938"
     CDS             complement(7085..7528)
                     /locus_tag="ACHGT5_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN7217912.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="ACHGT5_001939"
     CDS             complement(7529..8317)
                     /locus_tag="ACHGT5_001939"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN7217913.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACHGT5_001940"
     CDS             complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACHGT5_001940"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN7217914.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="ACHGT5_001941"
     CDS             complement(9432..10019)
                     /locus_tag="ACHGT5_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN7217915.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACHGT5_001942"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACHGT5_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN7217916.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="ACHGT5_001943"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="ACHGT5_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN7217917.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="ACHGT5_001944"
     CDS             complement(12936..13871)
                     /locus_tag="ACHGT5_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN7217918.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZBWR010000076        13917 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000076 DAZBWR010000000
VERSION     DAZBWR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.078"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..192)
                     /locus_tag="ACHGT5_001945"
     CDS             complement(<1..192)
                     /locus_tag="ACHGT5_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN7217919.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESP"
     gene            complement(671..1390)
                     /locus_tag="ACHGT5_001946"
     CDS             complement(671..1390)
                     /locus_tag="ACHGT5_001946"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN7217920.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1439..3085)
                     /locus_tag="ACHGT5_001947"
     CDS             complement(1439..3085)
                     /locus_tag="ACHGT5_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFN7217921.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3264..3854
                     /locus_tag="ACHGT5_001948"
     CDS             3264..3854
                     /locus_tag="ACHGT5_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN7217922.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3946..5451
                     /locus_tag="ACHGT5_001949"
     CDS             3946..5451
                     /locus_tag="ACHGT5_001949"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN7217923.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5535..6887)
                     /gene="celB"
                     /locus_tag="ACHGT5_001950"
     CDS             complement(5535..6887)
                     /gene="celB"
                     /locus_tag="ACHGT5_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN7217924.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6887..7405)
                     /locus_tag="ACHGT5_001951"
     CDS             complement(6887..7405)
                     /locus_tag="ACHGT5_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217925.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7421..7747)
                     /locus_tag="ACHGT5_001952"
     CDS             complement(7421..7747)
                     /locus_tag="ACHGT5_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN7217926.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7760..9739)
                     /locus_tag="ACHGT5_001953"
     CDS             complement(7760..9739)
                     /locus_tag="ACHGT5_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN7217927.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9760..10074)
                     /locus_tag="ACHGT5_001954"
     CDS             complement(9760..10074)
                     /locus_tag="ACHGT5_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN7217928.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10242..10535
                     /locus_tag="ACHGT5_001955"
     CDS             10242..10535
                     /locus_tag="ACHGT5_001955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN7217929.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10613..11725)
                     /locus_tag="ACHGT5_001956"
     CDS             complement(10613..11725)
                     /locus_tag="ACHGT5_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN7217930.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11878..12726
                     /locus_tag="ACHGT5_001957"
                     /pseudo
     CDS             11878..12726
                     /locus_tag="ACHGT5_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13079..>13917)
                     /locus_tag="ACHGT5_001958"
     CDS             complement(13079..>13917)
                     /locus_tag="ACHGT5_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN7217931.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4340 a         2843 c         2385 g         4349 t
ORIGIN      
        1 aggactttcc ctcaagctag ccaagaaccc atctgctgaa atatgaaaat ttttgaatgc
       61 tacgacattc gccattacag gtatgtaaaa gaaaaaaatc atccaaagca tacccggtac
      121 agccatcagg acaagtgctt tataacgcca tagattcata aaaaaatgat tgaacccctt
      181 tcgttttttc atctaaaaac ccctttcgat tcattcgcaa cgtaattata gcgctttcaa
      241 taatgagaat aaataaacaa agtataggga aaatgaaaca aagtatagga gaggttgtga
      301 aatcagcgtt ttggcctgag tattagagaa aaaaacggga aaatagttcc tcatattttg
      361 ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca gaggttgtga
      421 attcagcgtt ttggcctgag tattagggag atcgtgtcct tactatctaa ctctgagtca
      481 caaggaacaa tggggactgt aacaaaagtc ttgtcacagt cccttattcc gaacaaacgg
      541 tgttccagaa gtagcttcta cggaaataag ccgaaatttc acaaaaaagc catccgaaag
      601 tcagacggac aagtctgctt ttcagatggc gctacaacta ctctgcttat tcttttagct
      661 atccattcaa ttatttttct ctcacttgtt ctagctcttc tttggtcatg ggtcgatatt
      721 gccctttatt caaatcttta ggcagccaaa attttcccat acggattctt tttaaatata
      781 agacttcttt tcctactgct ttgaccattc gcttaacttg gtgaaacttt ccttcatgca
      841 aaatcagacg gataattgaa gtttctgtct cttgatctac ttgatcaatt tggagttttg
      901 ccggcaatgt ctgctcatcg ccatcaataa tcagtccttc tgcaaataaa cggatatctt
      961 cttctgtcat tataccgcta acctcagcta gatattcttt ctccacatgt tttttaggtg
     1021 atagtaactg atgggcaaac accccgtcat tcgttaaaat caagaggccc tctgtgtctt
     1081 tatctaatcg gccgactgga aaaagatctt cccgataatc ctggtcttcc aataaatcga
     1141 ttacggtttg atcatgatta tcctgagtag cagaaatgac tcctgcaggt ttatgcaaaa
     1201 tgtagtaaaa atctttttga tacgtaatga tttctcctaa ataagtaacc gcatcttcga
     1261 attcatttac ttgcgcttta tcgcttttca ccacttgtcc attgacttcg atcgttcctt
     1321 ttttgattaa tcctttgact tctttgcggc tgccaagtcc catatctgct aaaaacttat
     1381 ctaaacgcat gcgtttcccc catttcaaaa gagcatcaat ctagctcttc tgattcactt
     1441 attttatttt taatttatgt cgtaaacgcg ctattcctgg tcctagtagt ctttctgcca
     1501 agcggatttt caaagctaaa taaccataca ctaaagcccc tatacctgct actacaagaa
     1561 tcaacaatag agactggaat ttgctatctt cgtttaaaaa agcaccaaaa atcatctttg
     1621 tgatccacga agccaaaagc ataatcaagg taagaataaa gatcagcaat gtcctgcgcc
     1681 agacaaaact tggtttaaag cgggctactt catggattct ctttaagatc aaggtacaag
     1741 acacagcaaa tccgatcatt gtcgccaata aagggccgta tacttcaaat aaacgaatgg
     1801 ctgggtattg aagaaggagt ttcactagaa acccaataac caaataatta attgccgcct
     1861 tgttttcaaa cattccttgc agcatgttgg aaactagcat atatagtcct aggaagagtc
     1921 ctacaaaact tgcttggatc agtacattac ttcctagaga atccggtgta taaaataatg
     1981 tatttagcgg ataggcaagt aacataacac caaatgtcgc cggaaacata aaaaatgaaa
     2041 acaactgtaa atttccgctt gtcaattttg ccagacctac tcgatctttc aacgtaacag
     2101 cttctgtaat caacggtaac ccagtagacg cgatcgatgt tgccaatgca ataactacca
     2161 tcgtcaactt atctggattg gcactaaaaa tcgaaaaaag atccagtaac tgtgcattgg
     2221 aatactcggt ggatgcagac atgatcttca taaaagtgaa ctgatcgact aatttgaaga
     2281 tcgttacccc tgaacccaca ataataaacg gaatcgcttc tttaacggta tctaacaaca
     2341 attccttcgt tgcaatattt attttgtttt cgctgtagtg aataaacgcc gatgtatacg
     2401 cttgatgttt cttcaaaaaa tacaaaagga caagaatact tgctagcata ccgataaatg
     2461 ccgcgaaagt tgattgcgtg accgccgtca catagtcacc atccatgact ttcatgatga
     2521 tgaatgtagc caatagcata taaaaaatac gtgccacctg ttctacaatc tgcgacagcg
     2581 cataaggcat catttcttga ttgccttgca aataccctcg tatcacactc atacaaggaa
     2641 aaaccaatac agcgatactc aaagaacgca tcgttggaat cagttcttct ccgccaccag
     2701 aagcatgagc tagccaagga gaggcgatgt acatgaacaa agcaaacaac aggcctaacc
     2761 cgcccatcat ctgtaatgca cggataaata atcgtcgtga agtgccatat tcattcaatg
     2821 aattatagcg tgcagtttgt tttgcaatcg cagcaggtat tcctgctgta gagatcagta
     2881 gaaacaatgc atatatattg tatcccatat tgaataatcc attggcagcc ttagcatttt
     2941 cgcccatcca agcataccag ggaatgatat agacagcgcc tagcaatctt gaaatgatat
     3001 tactggcggt catccaagca gagcctcgtg ccattttttc ttgcggtgtt aattgatcta
     3061 atgctggttt tctttgatta tccatcggtc agattccaac tttctacaaa acattactat
     3121 cattttaatt ggtaaaacag aaactggcaa tcgattttat ataactttta taagaacaat
     3181 tgtttttttg aattcggttt cgtttttttg gtaaagtaaa gaaatataca gccttttatg
     3241 gctaacattt ataggagata tcaatgacac aagaaataaa aaaactaccc gttatatttt
     3301 taggtctttt ctttatcaca gttggattga attggttctt gctccctcac gatatagctt
     3361 ccgccggagt gggcgccatc ggacatttag ttgaaacggc cttttctatc caacgctcag
     3421 ttactgtttg gagcatcaat cttatgatgc tgcttctagc cgctgtccta ttaggaaaat
     3481 cagtattttt taagactgta gcgggaagtc tgctttttcc tgttgtttta gagattgttc
     3541 caaaagtgga aatgatctcc tcacatttct tttcactgat ttcaggaagt cttttgttca
     3601 gtcttggggt ttacacattg tacacagtcg gggcctcaaa tggcggtata acgataccgc
     3661 cgattatttt ccacaaattt tttcggcttc caatgccgca aggactttta cttaccaata
     3721 gtcttatcgt ctttttgaat tattatgttt ttggattgtt ggagacacta ttcgttctcc
     3781 tatctattac agtcagttca ttgtttatga aaatactcat ccgcttgtcc cctgtgtcaa
     3841 gaaaatttga ataaagctgg attttgtcat agcggaaaca aacgatatat taaaaagagt
     3901 gtgctataat aaaccaagaa attgaataaa gcgagggatc aatcaatgag cttctcttta
     3961 gaagacatcc gtcatttact attaaaagaa aatctattaa aagaatttgt ttcaacaaga
     4021 ggctggcatc ttgatgtacc agaaaaagct gaatttcttc agttaagtta tgattcaaga
     4081 aaagctgatg cttctacttt gtttttctgt aaaggaatga atttcaaaga agaatatttg
     4141 gattcagcaa tcgaacaggg tattcaatat tatgtatcag aacaaccata cgaaaattca
     4201 gcagtaggaa tcatcgtaac ggatatccgt aaagcaatgg cgcttcttgc tatggctttt
     4261 tacgactatc cgcaaaataa attgaaagtg atcggattca ctggaacaaa aggtaaaaca
     4321 actgctgcgt actttacaaa agcaatcttg gatcacacaa caaataaaaa aacagcattg
     4381 ctttctacta tgaatacaac gttagatggc aaaacatatt tcaaatccca tttgacaacg
     4441 cctgagtcat tagatttata tcgtatgatg tcagaagcag ttgaaaatgg tatgactcat
     4501 ctagtgatgg aagtttcttc acaagcttac aaaacacaac gcgtatacgg cttgacttta
     4561 gatgtcggaa tcttcttgaa tatctcgcct gaccatatca gtccgatcga acatccgaca
     4621 tttgatgact atttctattg caagcgacaa ttgatcttga attcaaaaac agttgtttta
     4681 aatcatgaaa gtgactattt tgatttactt agggaaacag ccgatctatt ccagattcca
     4741 acgatcactt atggtcgttc ggaagaaagc aattatcaag tgatccgttc agataaaggt
     4801 actcacggtt ttactttatc ttctcatgaa gatcgcttag ctatctgcga tactgcttat
     4861 gatattttgc tggctggagg attcaaccaa gaaaatgcag ccagtgcgat cattgccgca
     4921 gcactaacag gtgcctccag tcaagatgca caagacggat taaaagaagc tcgtgtacca
     4981 ggccgaatgg atcaattgat ccaatccaat ggagcgcatg tctatgtgga ctatgcacat
     5041 aattatctaa gcttgaaaac attgctgaaa tttgccaaaa atgaacatcc agatggccgt
     5101 gtaattgttg tattaggcag cccaggaaac aaagcaatct cccgtcgaca tgattttgga
     5161 aaagtgctat cagaaacagc tgacatcgcc tttttgacgg cagatgaccc agcatttgaa
     5221 gatccgaaaa aaatcgcgga agaaatcaat gaagcaatta ctaatccaga tttgatcgtt
     5281 cattatgaaa tggatcgtcc tgaagcgatt cgacgcgctt tagcagaaag cgctccgcaa
     5341 gattcagtcg ttattgcggg aaaaggcgtg gatccctatc aaaaaatcaa tggtgtagat
     5401 gaaccttatg aaggggatta tgcaatcgtg aagcggttga ttgaagaata atggtattta
     5461 tatactagtg gtttcccatt tctaagaaaa aaagggactg tgattttttg tcacagttcc
     5521 ttttttctta gaaatcattt taacttttca ctagctaatt ctgctgcctt ttctctttct
     5581 tcatttaata atttattatc atagaatttg ataaatggga accagataat gaatgcgatg
     5641 attgcacaaa tgactgctaa cacagcagct ttccaatcac caccactacc aacaaatgct
     5701 ccgatcccga ctggtgaagg ccacggcatc tgtgcaagca tcggtttgac gatttccagc
     5761 cgaatggcaa aataagcgag tgtagcagaa gccatcggag ctaagaaaaa cggaaccgct
     5821 aaatagggat tgtagacgat tggcatcccg aaaatgattg gttcattgat attgaataat
     5881 cctggtacaa gtgaggcttt tcctaacact ttcagctgat cagatttcgc gaggaatgca
     5941 ataaagatga tcaatcctaa agtcgcacct gatccaccaa cggttacata agaattgttg
     6001 aattctcctg ccaacggata atttgcgcct tgtgcgttag ctgccatatt agctaatacg
     6061 atcggggtca agaaagaagt gatgatattc gctccatgga tccctacgat ccacaatgca
     6121 tggatcaaga aataaatcac catgacacca agccatgtat tcgtcaactg cgtaacaaac
     6181 ccaaatggga tagcaatcat tttgaagata tccgttccta gtgcaactaa taatccattg
     6241 atgatcatca ccacaaacgc aatgacaaat gttgggatca gagcagtgaa agaacgagaa
     6301 actcctggcg gaacaacatc tggcattttt atgacccaat ttctcttgac acacatacaa
     6361 tacaattcga ccgcaataat cgacataata atagctgtaa agattcctga agttcctaaa
     6421 cgttcgacaa aagagcccat cctaatacca tttactacag tttctgtctc attgatagaa
     6481 gcaatcaagc tgattttacc atcagtcatg atcaattcag ggatacacat aaagaatgca
     6541 aaaacagata gtaatgcccc cgtgagcgga ttaacattta atttttgttc atctcgttca
     6601 attgacgtta attcataagc aaacacaatg gcaaagtaaa tagataaaat ccccatcgtc
     6661 attgtattga cgatcatgta caagtcacta actttgaaaa aagttgcatt aaaaaatcct
     6721 tctaaaaatg ggaacggtaa tggcaaaata tttaaaacca aaaacatcga gcctacaatc
     6781 gtgaaaggaa tagaagccat ccccgctgcc atgacagccc gaacgatttt aagttgcgca
     6841 attttcccca taggtcccat caggtatttc tgcaaaaaat caaacattag ttattctcct
     6901 ttcctaaata taacgctttt tcatattgtt taatacggat atactgttgg tcttggttga
     6961 gtgaaatttt ctttaaacga tactgtataa atgcactggc aaaaataccg cttacaacaa
     7021 gaatgaaaac aaataactgt cctccattgt tatttgccat aaaaggatac aagaatgtaa
     7081 atcctgaggt aaacaacact ggaatcacta ataaattaaa gacactctgg aacaaaaaat
     7141 accactttgt ccatggaacg ttcgtttggt gttttcgata aagctttact tgttctccta
     7201 ctgcaggcaa aatagctaaa atcagaatca tcggtaaact acttcctaaa cttttagctg
     7261 atagaagcag aatcatccat tgcatgttca tgaaaaagaa aaaggcagtc aggtaacgta
     7321 cgattagata acgattgaaa tacatgtttt tcaaactaag ttgctttttt tctaattcga
     7381 ttttcctttt tactgtatcc tgcacaatga tccttccttt ctatttattt ttttgataca
     7441 aataattcat ttcctgtgca acttccaata atgtcatcgt cgtcatcaga tgatcttgtg
     7501 catggaccat gataatttcc atttctattt ttgtcccgct ggcatattct ttcagaagtt
     7561 gagtttgtga ctgatgcgca tgaagaatct cttcatttgc ttgtcgcaac ttctcttcta
     7621 cttcttcaaa catctcttta cgcatcgagc gaaatgcttc atgtatcagt gtccgtgcat
     7681 ttccgctatg gaaaatgata tcaaaagaag tcacttgtag tgtttcactg tttatttttt
     7741 cagccatctg ttttcctcct tattcgatca gtttgataaa tttactgctg aaattctcaa
     7801 aaataggttc agctaagatt tcctgctgta cttttagctg gtcaactaac ctaacgattg
     7861 ctttagtcac gaccgtaatt ccttcatttt cgacataaga aggcgaaact aaaaaaacaa
     7921 aatgaatttc ttgatattga tcccactgca tcccagaagg aatcaatgca acggctacct
     7981 ttgtttcgat tccaactgga acagcgggat gtgggactgc cactgtatca ctgaatatga
     8041 tttgtcccat attttcacgt tgctggatct gctttcgcat ttctcttata tatccttctt
     8101 cttcatttat gctcagcttt tgcagcaaat catgaagaat ttcttccttt ttcggttcgt
     8161 acgtataagt cttgaaaaat ttttcagaca actgttcggt gtaaatctgc tttttttgct
     8221 gtagagaaaa tgctggtatc ggtctatctg aggaatgata gggtatagat tctccaacta
     8281 cctttcgtat tttacgaaca tcatcttcat tcaaaaaaat actgacatgt aaaactggaa
     8341 ttttgaaaaa cattgtcgaa agatcaatag aagaaataat caggtcgatt ccttttaaag
     8401 ttgtttcatt gatctcataa tagcctttta cacttaccac tgtaatattt ttaccgaatt
     8461 cactgactac gcgattcttt aatagttggg cactgccgta acccgttgca cagataatca
     8521 atgcatgtag tttatgcgca gctctttctt tttccaatgc cgccatgaga tgcaaagcca
     8581 aataggccca ttcatcttca ttgatggtat agtttttcaa agccggcatg ttgctgaaat
     8641 gcctttttac tgcgtcaaag actttgggat tttctttctt tatctcctca gtcaatgggt
     8701 tctcaatgcg aatacctcgg ctcaatcgaa tcatcatcgg tgacaaatga tcgagtagtc
     8761 cattgaaaag ctggagatcc tctactaatg aaaaatgaaa ttcctgagac aatcttttca
     8821 gaactttcgc taattccttt gatagttcct gatcattttg attttctttg tgattcgact
     8881 tagccatgag atgtaatgtc aaataagcaa tttcttcttc gggaaacgat acacctgcga
     8941 ttacctcaat acgttgaacg atccgctgcg ccacttgata ctctactcgc ttagaaactt
     9001 cttgttcgat gcctaattcc ttgatttcaa acccttctcg gagccgtttg atccccaaag
     9061 ccaagtgtaa agtcagattc tgtatgataa aatccgaaac ttttaactta gcttctcttg
     9121 tttcatcgag aatgataatg actagctctt caaaactgat gtctgggaaa aaacggcttt
     9181 gccctaaata ttctttcaat gaatttccat aatggtttcc aaaaaaagta tccatgataa
     9241 aatgccgtcg atttctttct tccccttcga tccatgtccc ttgaccatgc ttagaaacga
     9301 tttttagtga atatggaatc aatcgctcct tgatttcttg catgtccttt gtcaaactgg
     9361 aacgactgat ataaagctct tctgccaaat catccataaa aagaatggca tcttccaaaa
     9421 gcaattggtt caatatatac ttttgtcgat cttcggactc ataaaatcct tgcggttctc
     9481 tagcatatcc aggtactatt tcttgccgac tcaaaaacaa gtcaaatgtc agtctttgta
     9541 aaatatgcag ttggtagcca taacctggtt tagcaatgat cttacctccg tttttttcta
     9601 ctaactcttt gatccgctgc aagtaattac ggattgtccg atcagataat aacaattcag
     9661 aagctaaacg ttgactggtg acatactcct tcttgtgatc aatcaaatat ctcaacagca
     9721 atttttcctt ctgtttcatt atcatccact cctattccat catatgttct cttcgatcac
     9781 ctctgctaac ttctggatgc ctgtagggat tgggacatac gcttggaaag ggatactaac
     9841 aacaggcttt ccaacagatt ctcctaattt tttgaattta tccaaaaaca tcgtcgtttg
     9901 cgggctgatc agaaataaat caaaatcact attcttaatc atattatcgc cttctgtagc
     9961 tgaaactgca tctacgagta cctcttctct tttttgctta aagaaatccg ttgtctttga
    10021 ggccatcatc gaggatgaca ttcctgccgc acaaataatc aatgcttttt tcataaatca
    10081 atcactcctt ttatttttat aaaccaagca tatcttgtaa gcgttttcta ttcaaaatat
    10141 atatttccgt agcattgcgg aaaaaatgtg tatcatatct tgaataataa tttctggcac
    10201 cgtgatatac tagtgataga attttttcag gaggggattt agtgagtatc acagtgaata
    10261 ttttgtatac aagtacaaat ggacaagcca gaaaatttgc agaagaaatg gtcgaaagaa
    10321 acattgtcaa agcgattcgt gcagaagaag gaaacgagaa atatgactat tactttccac
    10381 tagaagatcc agaatctctt ttactgatcg accgttggaa agatgaagaa gcaatcgaaa
    10441 agcatcacaa atccgaaatg atggctcaaa taaacgaact ccgtaaaaag tataaattaa
    10501 aaatgaaagt cgaacgtttc actgatttac cgtaaaaaga tacacatgga atgatagcaa
    10561 tttatcatcc atgtgtatct tcactttttc cttatttttt attcgatctc atctaatgat
    10621 tcaaaccgtg tttttctctt atttttcgga taagtatttc agtcacccca atatacatga
    10681 ttcctaaaca taccccaagg atattctcaa aaatgcggat cgttactgct cctggaaccc
    10741 cgaataaact ggcagtgatt gccaaagcac caaaagaatt aaaaactgtt ttatatctat
    10801 aggtagaaca aacacctaaa gctagaccac ctaatatacc gacccagtta aaaggaataa
    10861 attggctgat accaataaat aaaacacatc caatcaatga gccaatgatt cggtctactg
    10921 ctcgttcttt gaaaccaatc gacatcaatc catagctaga tacaatagaa gaaaatgcaa
    10981 accctgccca catcaatcgc tgaaaaggga catactctcc tgcaacgatc aaaagactaa
    11041 tgccaaatgc atagatgagc atccaaattt tttcctgcga atacatgcct ttcaaaaaaa
    11101 tctctttaaa caagcttttg tctttgttct tttctcgatg ttttctgtaa aggagaactg
    11161 aaaaccaaca aaaaaacaaa aataataagc tacttgtttg tgtaaagaaa ttcttgttca
    11221 acgaatcttt aggtaacgaa taaacaataa agagatatga aaagccgtac aaagaagcat
    11281 tccccatttt gggatcacta gcggttgcca ttaatatgct gcttagtaaa ataaaatgaa
    11341 tcaacatgcc cataactgac catttgatca gctggacata cggtgctact aatagtgaga
    11401 acatgacgat tgctaaacct atgatcgaat gggaaatctt ataaccaaag tcaacaaaac
    11461 gaatactcat caacatacag aataaaacca cagcgtatgg tttatgcttc gattcaaata
    11521 atgttgtaat tataatgata aaacaaatgg caaacaatac gatgaataat gatcgtaaaa
    11581 acaatgaaca aatatacatg cacttttctt ttttagaagc tgcttctcgt attttttgtt
    11641 ttaaaataaa cggatctagt tgtagagact gataaaaatc cacctgttgt cgtcctttct
    11701 ttttactttt attttttaca gtcatctatg acaggttgta aatactatgg aacatcatat
    11761 ggcgttgtca ttttcttgtc aactattttt cctattcatg tttttataaa aaaacatatc
    11821 gttttcttgt atgattaaat caccacaaaa taaacaactg agagaacgat atgctttatg
    11881 aatggttcta tcaaaaaaat tctgagatca atagacaaaa atttgatgat tacagaggtc
    11941 tcttacgaaa ccttacagaa gaaaaaaaca ttgatcgttg ctgcagttct cttccctgct
    12001 ccatgtgtct gtagaaattg tggttctgct gtggtagatg gaaataggaa attaatggtg
    12061 gtgaaaaaca agaagaagga aaccattgtc cactttgaat aatataatca tatgccaatg
    12121 gttatacgcc taaaaaagca gcgctatacc tgtaaaaact gtcgaagcca ttggaacgct
    12181 caaagttatt ttatccgtcc aaggcactcg atttcaaatc atgtcagaca taaaatcact
    12241 tctttactga ctgaaaaagt gtcgttattt tttatttcga aaagttgtta ggtatctttg
    12301 acaaccgtta ttcgcacatt gaaagagttg agaagctatt taccaaagtc ttccaaaaga
    12361 attctgtctt gagtgttgag ggttgtgaat ttcgttctca cgtttccgtt gaggataaac
    12421 tgagttttat ttccgcggat ggcgagacag aaaaattaat cgagaaaatt tacctacaca
    12481 aaaaattcct cgattaacaa actatttcct tagctgcact aatcctaaag aaatagaatt
    12541 cttagttaca gacatgaacg ccacctactt tcagctcacc aaacatgttc taccaaatgc
    12601 gaaattggtg attgatcgat tacttggttt ttccacgcct ttcaaggagg cgtaaccttt
    12661 attcacgagt tggttgaagc tttccgagac aaaagacccg acttgttttt ctccttattg
    12721 acaaagagcc attaaaacta gagaaaaaat tacaactgat cgctgattat ttgtagaaag
    12781 aaaatcaaag tattacccaa taaacttgag tcattaggca aaaaacactg cgaaactcaa
    12841 gaatgatttt cgcagtgttt ttgtatattt tttattaact agtattacta caaaggttta
    12901 tttttcactt ctcaacgcag cacaaaattg tctttactta tttactggtt gggttcaacc
    12961 ttctctactc cgatactaat atttgtttgt ataccccttc taaaatcggt agtttccatt
    13021 caattgatgg gtactagttg attttaaaaa ctatgcttat tcaatttttt attttagctc
    13081 atatatttcg tttctttcta aattttatac tgatgaaaac aagtagcaca cccaatattg
    13141 acaatataaa ctggctaatg atggtttctc cagtttttgg aagatgctta tttccactgc
    13201 ttgttggatt gtttcctctt atatctgagc tatcagcaac ttttctattc tcttttgtta
    13261 aaaagatcgc cacttcttgt tctttttcga tcttgaaagt tactggtgtc gcatccaatt
    13321 cataccccgt tggcgcttga gtttctacca gctgatatgc tcctggtgct aagccatcta
    13381 tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt ccattggcat
    13441 cttgtaactc aaaaacagct ccttgcagga cttctccgct ttggtcatcg atctttgtca
    13501 acacgacacc gcctggtgta agtcgatttt ctttcgtcaa ttctaccacc gctgtctgac
    13561 ttctttcgat ctcaaactcg attggtgtcg catccaattc ataccccgtt ggcgcttggg
    13621 tttctaccag ctgatacact cctggtgcta agccgtcaat cgccagtttt ccatctgctc
    13681 ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc
    13741 cttgcaggac ttccccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    13801 gtcggtttgc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    13861 tcggtgtcgc atctaattca taccccgttg gcgcttgagt ttctaccagc tgatatg
//
LOCUS       DAZBWR010000077        13789 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000077 DAZBWR010000000
VERSION     DAZBWR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.079"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            444..674
                     /locus_tag="ACHGT5_001959"
     CDS             444..674
                     /locus_tag="ACHGT5_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217932.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            833..1666
                     /gene="terS"
                     /locus_tag="ACHGT5_001960"
     CDS             833..1666
                     /gene="terS"
                     /locus_tag="ACHGT5_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFN7217933.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1659..3074
                     /locus_tag="ACHGT5_001961"
     CDS             1659..3074
                     /locus_tag="ACHGT5_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN7217934.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            3086..4615
                     /locus_tag="ACHGT5_001962"
     CDS             3086..4615
                     /locus_tag="ACHGT5_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN7217935.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4533..5585
                     /locus_tag="ACHGT5_001963"
     CDS             4533..5585
                     /locus_tag="ACHGT5_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFN7217936.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5587..5904
                     /locus_tag="ACHGT5_001964"
     CDS             5587..5904
                     /locus_tag="ACHGT5_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217937.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            6031..6654
                     /locus_tag="ACHGT5_001965"
     CDS             6031..6654
                     /locus_tag="ACHGT5_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFN7217938.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6669..7559
                     /locus_tag="ACHGT5_001966"
     CDS             6669..7559
                     /locus_tag="ACHGT5_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFN7217939.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7582..7797
                     /locus_tag="ACHGT5_001967"
     CDS             7582..7797
                     /locus_tag="ACHGT5_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217940.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7809..8150
                     /locus_tag="ACHGT5_001968"
     CDS             7809..8150
                     /locus_tag="ACHGT5_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217941.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8150..8521
                     /locus_tag="ACHGT5_001969"
     CDS             8150..8521
                     /locus_tag="ACHGT5_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217942.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8514..8909
                     /locus_tag="ACHGT5_001970"
     CDS             8514..8909
                     /locus_tag="ACHGT5_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFN7217943.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8911..9288
                     /locus_tag="ACHGT5_001971"
     CDS             8911..9288
                     /locus_tag="ACHGT5_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFN7217944.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9289..9897
                     /locus_tag="ACHGT5_001972"
     CDS             9289..9897
                     /locus_tag="ACHGT5_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN7217945.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9897..10238
                     /locus_tag="ACHGT5_001973"
     CDS             9897..10238
                     /locus_tag="ACHGT5_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217946.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10480..13269
                     /locus_tag="ACHGT5_001974"
     CDS             10480..13269
                     /locus_tag="ACHGT5_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFN7217947.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13259..>13789
                     /locus_tag="ACHGT5_001975"
     CDS             13259..>13789
                     /locus_tag="ACHGT5_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN7217948.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKD"
BASE COUNT         5025 a         1876 c         2797 g         4091 t
ORIGIN      
        1 agtgaagaga aatgattcca ttaataattt caatttttgc gctttgtctt aatgtatata
       61 tgattggatt taaaaatggg caaaataaaa aatagtagca gccaagaata attttatagt
      121 gtcactgtgg cggaaagggt agacgctaag catgtgtgct aggtcaatgc ttcggcaacc
      181 atgcaaggtt cgattccttg ccagtgactt tggcaaccga ggcatcggcg gtttaaaaat
      241 ataggggtgc gcaatttcgt acgcgttttg tgcatcgtgt aggttgctat tacataactg
      301 gttaggttag attgagattt gggattcggt acaaatgaat cgtcaaatgt ctcaagcaca
      361 ggatcggaaa cgtccctgcc tgtgcattac atattagatc actctttgag tggtcttttt
      421 ctttacataa aggaggctac ataatgagaa actactggta tgtatcgcta actaatgaat
      481 atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa atcaaaggga
      541 agtactctat tgtcgaaatg gggagagaag cagaaccatg tgagattgat gcgtgcaagc
      601 tcgtttattg cggtcatggt ttctatgatg aaccaaacat tcaaaataat attaacaaga
      661 atttgaggga ttagaatgca agaaatggct taaaaatctc caatcaggac aaatcaagtt
      721 agacaaagtt tccgatttaa agatattgat tgaagctgat ttaatgctaa aagatattga
      781 aaattagaaa acaaaactca acctaaacag aatgtgaggt ggtgtgtatt gaatggcaag
      841 acaacgtgat ccaagacgtg atgaagccaa aagaatttgg ttagaatcca acggagaaaa
      901 gcagttaaag gaaattgcat ctgaattaaa tgtttcagat tctcaggtta gaaaatggaa
      961 atcgcaagac aaatggagcg ctgaattgat tagtaacgtt accaatggca aaagtaacgt
     1021 tactaatcaa ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca aaggaaatag
     1081 ccgagcttct ccgccagtgg gtaataaaaa cgctttgaaa acaggtgagt atgaaacaat
     1141 attttttgac acgttaaccg ataatgagaa ggacatctat tctagtttga atgatgatcc
     1201 ttcttttgct ttgtccgaag aaatacgact tcttaaaata agacaacttc gaatgatgaa
     1261 aagaatccaa caagctgaag ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct
     1321 aagaaagatt aaaactccga ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt
     1381 tatgcaagat gtgcagatta gcagaaaaaa acatcgcaaa attgatgata ttctttcaat
     1441 tgaagattca ttgactcgga ttagcaacca gttagctaaa gccatcaagc aaatgaatga
     1501 actttatatg aatgaataca gaactgattt aattaaagct cagactgata agatccaagc
     1561 tgagacaaat gaaattggcg gaaataattc aggtgaagaa atagaagaat ggaaacaggc
     1621 agttttaaat gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa tttattcctt
     1681 ttgctgatat tggttctgcc attgattatt actatgataa accagtagct ttttgccaag
     1741 atattttgca tttgaatcct gatgaatggc aagaaaatgt tttaaatgat ttagctgaat
     1801 tttcaaaggt ttctgttcgt tctggtcaag gagttggaaa aacagcatta gaagcaggag
     1861 caatactttg gttcttaacg tgtcgaccct acgctaaagt aatagcaaca gctccgacaa
     1921 tgaagcaact ttacgatgta ctttgggcag aggtagctaa atggttaaat gatagcttga
     1981 tcaaaaactt actgaagtgg acaaagacca aaatttatat ggttggtgat tcagagcgtt
     2041 ggtttgctac ggctagaaca gcgactaaac cagaaagtat gcaaggtttt cacgaggacc
     2101 atatgttgat tgtggtagat gaagcttctg gtgtgtctga tccaattatg gaagctattc
     2161 ttggtacgct atcaggtttt gataataagc tgttgatgtg tggaaacccc aataatattg
     2221 aaggtgtctt ttatgattcc cataattcag accgtgataa atacagagtt cataaagtat
     2281 caagctatga tagtaaacgt acaaacaaag acaatataga aatgattctt aaaaaatatg
     2341 gaaaagaaag tgatgttgct cgtgtccgta tttttggaga atttccgaaa ggtgcgttgg
     2401 attcatttat cagtcttgaa acagttgaat tggctacaga aaaacaaatt agtgattctt
     2461 tagtcaataa aacaaaggtt gctcatattg gtgtcgacgt agctcgatat ggtgatgatt
     2521 ctacgattct ctttcctaga attgctacca gggcattgga gtatgagaag tattcaaaac
     2581 gtagcaccat ggaaacaaca ggatatgtca tcaacatggc caagaatcta atgagtcaat
     2641 atccgagtat tgataaagtg atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa
     2701 ccgaccgcct agaagaactt atagaagaca aacattatcc ttttgaggtg tttggagtga
     2761 ataacggttc aacatcagaa gacgattttt acgataattt aggtactcaa ctatggggaa
     2821 acatcaagga aatgttagaa gaaaatatga cagcaaatct taacggagaa cagcctgtta
     2881 ttgaattgcc ttctgatagt tcgttaatca aagaattaag tactcgcaaa ttcaaaatga
     2941 caagtagaag tcgtatacgt ttagaaagta aagatgatat gaaaaagcga aatattggta
     3001 gtcccgatat tgctgacgca ctggctttag cgttttatga gccaccaagt cactatcaat
     3061 ttattcaatt ttaggaggtg agcttttgat tagtacagta ttatctttag agagatataa
     3121 gaagctacgt gttaaatatg caacacaaat tgaagatgga atgtttgatc cgaatggttt
     3181 tatagaagat atgaaaccat tttttgctga tcgcgaaaga aaatatctag cttatactag
     3241 cgaaaagaat gaaatagata atagaccaaa gcctaacact gatattgtaa aagttaataa
     3301 taaacttcat gctggtatgt attcaattgt cgtagaccaa gcagtcaatc attttactgg
     3361 tatacctatc aaatgggatt acgatgtatc ggaacaaaaa agaacactca ttcaaagatt
     3421 aaaagataaa ttcttaaaaa atgatataga acttccgaca gttccagaag cttttaacaa
     3481 attaacaagc aatcttgatt ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt
     3541 tcaaggagct tgtggagtag ctttccgatt gctagagcct gtggaagaag atgatggttg
     3601 gaagttaaga gcaagtaata ttgaaccttg gagagcagaa agatatggaa atgccggaat
     3661 ctatatcaaa gaaaagtatg actcttacca gaaaaaattt tttcaagaaa tgaaagttat
     3721 gacaagaaat aaaattctga catatgcttg ctatggtgat ttgaattttg ttacaagcgg
     3781 aacgttcaaa aaaattgatg agaccgataa ccctttgggg acgattacct tggcagaatt
     3841 taaaaataat acgaatcgtt attgtgattt tgaagtagct gaggaaattg gagatgcaat
     3901 tgatcgtgct ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg cgtacatgct
     3961 tgttactggc acaacaatga gcaaaggcac tgctaaagaa atgatgaatc agctagggat
     4021 tatcaactta aaagccccta ctgcaaaagc tgaatatgtc acaaaaaatt tagcaaaaga
     4081 tttcaatgaa tatcatatgg atctattgaa aaaacagttt tacaccattt gtaaagcaat
     4141 cgattttaac gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca agtggcaaat
     4201 cattagccta gaagcgaaaa caaatactaa agagcaatat tttagagaag gattgaaaga
     4261 atgcgcagaa acaattgctg cttttcttaa gtttcatgac aaagtagaaa ttgagcctga
     4321 aaaaattatt tttactttct ctagatcttt gccaactgat ttaagttatt tagctgaagc
     4381 tttacctaaa cttgcaccat acgtatccaa acgaactatt cagagccaaa ttccatttgt
     4441 aacagatgtt gattatgaaa atgaaatgat ggaattggaa agtggaagtg cttatccaga
     4501 tagtgaatac aattttggcg gaggtggcaa tagtgacgat agaaacgaag tattggacca
     4561 aacgtcgaga actggaggat caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat
     4621 taactagtgt atttcctgaa gcactgaaag agatacaagc aaaactatta tcacaagctg
     4681 acttacacaa tatcacttat ccagaaatga tggagtttta cagtacaagt aatcagaaaa
     4741 aatatcgtga atatgtggaa aaaaattata agtcattaaa aatgtatgat gcaaaataca
     4801 aagagtttat cgatgaattt tttccaccat ttgactatgc aaaagtcaat cgcttattac
     4861 aaatacgatc agatgtattt aaaattcttg cagaatatgc gatggatgca gatgtgaatc
     4921 aatatttttc tgatcgctta gaggaaattc ttcaaagaac atattcttct aatgctaatg
     4981 tttttgttca acttttaaac gttgatatac caaattattt accagaaaat gaaattaaac
     5041 attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg ggaaatattt
     5101 catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg
     5161 ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg tttaagttag
     5221 aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat gcaaaatttg
     5281 cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac aacgtattga
     5341 ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag aatccctata
     5401 aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta
     5461 cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat gaccgtttat
     5521 ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga
     5581 agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca
     5641 gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg
     5701 aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact
     5761 ccagttgata agaataccgt taatttaacg attcaattat tatctcattc attaccaaat
     5821 ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag aactgactac
     5881 aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc
     5941 gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg
     6001 cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc attaatgcca
     6061 atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa
     6121 tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt
     6181 gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc
     6241 atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa
     6301 ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt
     6361 gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa
     6421 tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt
     6481 tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa
     6541 atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa
     6601 acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt
     6661 gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga
     6721 ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt gctggaaaaa
     6781 cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt
     6841 cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta
     6901 acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta
     6961 atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga
     7021 aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag
     7081 gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca
     7141 ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag
     7201 gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg
     7261 agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc
     7321 aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc
     7381 caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat
     7441 ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat
     7501 actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa
     7561 agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact
     7621 aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt
     7681 catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata
     7741 gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa
     7801 gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc
     7861 cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat
     7921 catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag
     7981 tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg
     8041 atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg
     8101 gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc
     8161 agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga
     8221 agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc
     8281 ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt
     8341 aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg
     8401 agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa
     8461 gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta
     8521 atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa
     8581 ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag
     8641 ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag
     8701 ctagaaaact atcaagcggt cgaattgttg gagaagttat aaacaatgaa cattatttta
     8761 aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt
     8821 taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg
     8881 caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt
     8941 agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca
     9001 actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt
     9061 gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa ccctaataga
     9121 aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat
     9181 gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt
     9241 cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa
     9301 agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag
     9361 acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa
     9421 ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt
     9481 acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta
     9541 tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt
     9601 gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg
     9661 gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat tgaaggaagt
     9721 aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga
     9781 tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg
     9841 tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt
     9901 caaaatatca aacaacgatt aaattaacga aaaaagacga agaaggtaaa tatgagcaag
     9961 tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct gcaggagtta
    10021 tggaagaaat gcaaacagcg actgataagc aatcagttaa aaaagcttta agtcgtgctt
    10081 attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat tattgcaaag
    10141 gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct gttactgcag
    10201 gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca agtcaccttc
    10261 atttaagtat tcgattactt accgagaatt agatataaag acgcaattac tcgaagcagg
    10321 ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc tttttgcttt
    10381 tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca ctcaatttta
    10441 ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt
    10501 tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa
    10561 agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt
    10621 tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc
    10681 ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat
    10741 atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa
    10801 ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct
    10861 tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt
    10921 cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt
    10981 ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga
    11041 cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt
    11101 ttatctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc
    11161 aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt
    11221 aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa
    11281 ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa
    11341 gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta
    11401 tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg
    11461 atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt
    11521 ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat
    11581 tcattaaaga ttatgggggc aatagctgct gctaatccat ttgttttaat tattggtgca
    11641 gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga gcttagaaac
    11701 aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata
    11761 aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa tgcagttgag
    11821 ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt
    11881 aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat
    11941 agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata
    12001 aaagattcag ccagagaaaa atggaatgga atcaaaggtt ctattatgtc agttgctggt
    12061 ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta
    12121 tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt
    12181 gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac aaaaaataat
    12241 atgattgctg tttggaataa tattaaagaa agtgccataa atatttggga atctataaag
    12301 aatatatttg taagttattt tacaaatatt tattttgctg cactcaatat ttggacaggg
    12361 ttcaagctta cattgataaa tatttggaat gaagtggtaa atcaagctaa atcgatttgg
    12421 ttaaacataa agtatttttt tattaatctt tggattgata taaaatattt tgcaattcag
    12481 aaatggattg aattgaagtt ttctattatt caaacttgga tcgatttaaa atataatgcc
    12541 attactactt ggaataatat taaacagttt ttcaaagata cttggaaaaa tattaaagat
    12601 acggcataca atacgtggat ttctataaaa aataccatga ttaatacatg gaataacatt
    12661 aaggactctt tctggaatat tgttactgga attgttaatt ctgctgaaaa tgcatggaca
    12721 aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa aagaaatctt taattcgtta
    12781 agggaagtca atttatttga aattggcaag aacatcattg atggacttat aaatggtgta
    12841 aaagaaaagt ggaatgcttt gaaagatact atcaaaggta ttgcaggtaa tatcaaaaat
    12901 tggattaaag gagctttaga tattcattct ccatcaagat ggatgcgtga tatggttggt
    12961 aaaaatattg ttcaaggtat tatcgttggt attgataaag agcaaagtaa gttagatcaa
    13021 accatgacaa atttagtaaa aacaccgtct gttcaacctg tagtaaatgg aataagtcac
    13081 ctaccaatta cacaaacaaa gcaaaatata tctgctagtg aaacaaaaga aattcattta
    13141 catttaaatg tttatggaga tttacctgat tcaatggttc ggaagattac cacaaaaatt
    13201 aaaacagaat tgacaagaca aatgaaacga gatgctggtg cagtaggagg aacattatat
    13261 gcaacttaag agaggacaat tttttattaa tcaacattat tcttctgaat ttaatgtgta
    13321 tattcaaaac aggcctgctt ctgtttcagc tagtcgagta attgaattga gagagcgaga
    13381 aggaaatgat tctatcatta ttgataaagg ctattacaaa aatgtgacta gaaagattga
    13441 gtgttactat aaagcacctt caattgatat ggtgcaggag tgggaagatc gaatcactga
    13501 atggttagat atgtcgtctt atagtgattt tattctttac tatgatgaac aatatattta
    13561 tcaagctgta gtaatagaag gacctgaatt taaaggaaca agaaaaacag gaaatattgt
    13621 cccttttgaa tttacagtga gtattcgacc atttaaggaa aattacaatg gtagatttac
    13681 tattcgacaa acagaaactt ttgaaattta taacccagag aagtattctt caaaaccgct
    13741 tattaaattg agtggttctg gagatgcttc tttttatatt aataaggat
//
LOCUS       DAZBWR010000078        13732 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000078 DAZBWR010000000
VERSION     DAZBWR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.080"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACHGT5_001976"
     CDS             complement(143..1345)
                     /locus_tag="ACHGT5_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7217949.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACHGT5_001977"
     CDS             complement(1348..1542)
                     /locus_tag="ACHGT5_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN7217950.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACHGT5_001978"
     CDS             complement(1589..2437)
                     /locus_tag="ACHGT5_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN7217951.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACHGT5_001979"
     CDS             complement(2642..2992)
                     /locus_tag="ACHGT5_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217952.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACHGT5_001980"
     CDS             3170..4033
                     /locus_tag="ACHGT5_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7217953.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACHGT5_001981"
     CDS             4657..5649
                     /locus_tag="ACHGT5_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217954.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACHGT5_001982"
     CDS             5665..6549
                     /locus_tag="ACHGT5_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217955.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACHGT5_001983"
     CDS             6598..8205
                     /locus_tag="ACHGT5_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7217956.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACHGT5_001984"
     CDS             8206..9690
                     /locus_tag="ACHGT5_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN7217957.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACHGT5_001985"
     CDS             9867..10859
                     /locus_tag="ACHGT5_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7217958.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACHGT5_001986"
     CDS             10911..11780
                     /locus_tag="ACHGT5_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN7217959.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACHGT5_001987"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACHGT5_001987"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN7217960.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAZBWR010000079        13569 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000079 DAZBWR010000000
VERSION     DAZBWR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.081"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..2291
                     /locus_tag="ACHGT5_001988"
     CDS             93..2291
                     /locus_tag="ACHGT5_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7217961.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(2873..3745)
                     /locus_tag="ACHGT5_001989"
     CDS             complement(2873..3745)
                     /locus_tag="ACHGT5_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7217962.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            4045..5019
                     /locus_tag="ACHGT5_001990"
     CDS             4045..5019
                     /locus_tag="ACHGT5_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7217963.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            5041..5733
                     /locus_tag="ACHGT5_001991"
     CDS             5041..5733
                     /locus_tag="ACHGT5_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN7217964.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5699..7207
                     /locus_tag="ACHGT5_001992"
     CDS             5699..7207
                     /locus_tag="ACHGT5_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN7217965.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7281..8384
                     /locus_tag="ACHGT5_001993"
     CDS             7281..8384
                     /locus_tag="ACHGT5_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN7217966.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8387..9724
                     /locus_tag="ACHGT5_001994"
     CDS             8387..9724
                     /locus_tag="ACHGT5_001994"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN7217967.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9711..10589
                     /locus_tag="ACHGT5_001995"
     CDS             9711..10589
                     /locus_tag="ACHGT5_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN7217968.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10586..11509
                     /locus_tag="ACHGT5_001996"
     CDS             10586..11509
                     /locus_tag="ACHGT5_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN7217969.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11506..11916
                     /locus_tag="ACHGT5_001997"
     CDS             11506..11916
                     /locus_tag="ACHGT5_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN7217970.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            11920..13488
                     /locus_tag="ACHGT5_001998"
     CDS             11920..13488
                     /locus_tag="ACHGT5_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN7217971.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4535 a         2231 c         2862 g         3941 t
ORIGIN      
        1 ccaacacaat ttgacgaaga gccttaatta gatgagtatc aattttacag aaaaatgaaa
       61 gccttttatt ttaaaaggag gagaggaaga aaatgaagaa aagtacagta ttttataaaa
      121 tatttttacc gatgttttta ttaggagccg tacttgtcat aggatttagt ttattcattt
      181 accagaatac ctatgaatcg atcgaatcga gttatttaac agacaaaaag aatttgctca
      241 ggcaagttaa aactaacgtt gaatggaaaa ttagaacgat tgaatattcg ttttctacgt
      301 atggttcgac caaaaatttc tcggacatat tcaagcatcc gctgacatat acagattact
      361 ccacttattc tgaaatcagg aaagaactaa attttataga aacgattgtt atggatgata
      421 ataattatga tttagtcagt ttggctggta aatggggagt aatcaatgga tcgttgagtc
      481 aactgaccga agaggaagtt gaaaagtatc aacagaagta cataaataac aaaaataatt
      541 tattttggca aaagaaaaac aaaggaatcg aaatggtgat ttctttgcca atgtttgaaa
      601 aagaaaaata tgcattggga attgctacga tagagaaaca tacaatagaa caagttgttg
      661 gtgaaagtgg agaagacata ctttctattg aaaacgaagt tggcaacctt tttactactc
      721 agacagtaaa aaataaagag caaccaactg tattaacgga cgtggatcta gattacaatc
      781 agctgaaagt gataagagaa aaagataaaa cgtatatagt actgaaatca gattataata
      841 attggattta cagattagaa gttgactcta gtgcaattgg cgcaatgatc cgcaatctga
      901 gaatcgggct agttgcagtg tctttaacac tggtaggact cattggtctt ttttcctact
      961 tattttcgga cagatttgcc cgtccgattt ctcaaataca ggaacgtctg aatcttaaat
     1021 caaaaggatt cagtgggaaa gaactagatt tggtcgctca atcagtcagt cggatcatag
     1081 gagaaaaaga agcgttaagt gcacacttag ttacccaaaa gccacagctg gaaacgctat
     1141 ttgtacttag tttatttaga aatcgagtag aaaggcgaga actagatcag cggttgcagc
     1201 aatttggtta tagttctcaa aatgactgct attatacagc tttggttcaa atcgatctgt
     1261 tagaagatag tcacggtggg gagcgtgatt tgctactttt agcgatcaac aacatgatca
     1321 cggaaattgt gccaaaggaa aatcgaatgc tgccgatcgt tttgaatgaa gaaatgcaag
     1381 ccactattta tcgaatggat aaagatgatg cagaagcaag caaaaaagtc atggagtatt
     1441 gccgactcat tcaaaaacgt gtaaaagaat acttgaattt aactatcagt ataggcatca
     1501 gtaaccggtt cgaaacgctg aatgaaagca agcaatcggt tgatcgggca aaagaagccc
     1561 tgtattatcg tgtaaatact ggtccaagtt caattatttt ttatcaggaa atcgttcctg
     1621 ctgctcatga aaaaacactt attagatatc cgatagaaga acaaaatcga ttgtttgaag
     1681 ccattcgttc tggaaatcag gaagtgaaga atttagtaca tgaattgatt gatgcattgt
     1741 ttgcacaaaa taaaaatcct ttgagtcggg aagtagtttc ggtccgcttg atcaatgaac
     1801 tcgttcaatt aggacagtta ttgggagtag attcaaaaaa ctttgataac atgaaacaaa
     1861 tttatgtaaa agcactgaac aattatcatc ccaaagaact agaaaaactt gttttagaac
     1921 aattagttct gccaatcaca gaatgctctc aagcaatcac tgatcatgaa tttaaaaatt
     1981 tgtcggagaa tattatgcat atcgtctaca cagaatatga tcgtgatctg tctttagatg
     2041 tgatcgctga taggttgcat tataacccta actatttaag taatgtattt aaaaaggaaa
     2101 caggagaaaa atttggtgat tttgttcaaa atcatcgttt agaggtagca aaaaaatggt
     2161 tgaaagagac taacctctcc gtaaaagaga ttgcagaaag attgcaatat cgaaatccgc
     2221 aaaattttat tcgtttcttt aaaaagaagg aatcgattac gccgggagaa tatcgaaaaa
     2281 aatatttgtg aatcaagagg ctgtttttct tgtgatctga ttcaagcaat aaaagaggac
     2341 agcaaaaaac agcacttcat gttttgctgc tggctgattt tattgccgca gaatcaacaa
     2401 gataaacgcc gtttaatcaa gaggctgttt ttcttgtgga ctgattcaag caataaaaga
     2461 agttagaata accctagtat ttgtatttat ggatagttaa atttcaaggc taagcacgca
     2521 aaaagaactg tgacaaaagt cttgtcacag ttctttttta cgaataaatg gcgttcaaga
     2581 gacaggctat cgacaataag gcataatttt gtgaaatatg ggaagctatt tttcaaaatc
     2641 gcttcttatt gctcctgtgg gttactcgtc gcaatgtttg tgagagtttt tatcatcgct
     2701 caaggctgaa cgtctctttc acaacctctg atacacggtg ttccaggagt agattcttcg
     2761 aaaaataagc cgaaatttca caaaaattta agaagcaatt ttcgtaaatg ccttcttatt
     2821 tcttgaagct gaccacttct gacacaacta ccattttgcg ttcagtacat aatcaccctt
     2881 tgacagaacc tacgagtgct cctttgacaa aatatttctg aacaaaagga taaacaatca
     2941 gcataggcac tgttgcaata acgataacag ccattttaac tgtttgtgct ggtggaatca
     3001 catcaacggc actactttct gcttgcatcc cacttgatac aatgacgatt tggcgtaaaa
     3061 gaatctgtat cggccattta gatgaatcgt tgatatacaa aatagcagtc atataagtgt
     3121 tccagtaagt aacagcataa aaaagcgaga tagtagcaat agacggtaaa gctaatggca
     3181 gcataatttt gaaaaataca ccaaaatctg tgcaaccatc catttttgct gattcttcca
     3241 aactatccgg caaggcttgg aaaaagtttc tcataatgat catattcatt gcattaacag
     3301 ccacaggcag gatcatggcc cagtaagaat tgaccagccc tagagaacga acaactaaga
     3361 acgttggaat catccccccg ctgaacaaca tagaaaatac aacaagaaag ttgacgaaac
     3421 ctcgaccaaa caaatacttt ctggaaagac cgtaggccat cagtgaagtt gcacacatac
     3481 tgacgatcgt tccgacacct gtaacaccaa tcgaaacagc aagtgctcta aaaatggtcg
     3541 gcgtagaaag aatgtagcga tacgcgtcta aagaaaaggt agtaggaaaa aggataaact
     3601 ttttcgcgac gacttcttgt gtagaagcaa aagatgtagc gatgatattt aaaaaaggaa
     3661 cgatacagat caaagctagc aaaattagaa acgtattgtt gaaaagtgta aaacttcttt
     3721 ctcctataga gactttcttt ttcatataga taggctcctc ctcaaaataa attaaatgtt
     3781 tctgtttcta tatgttcaat ttatcatcgg tgaaagatta cgtatataat catttcctat
     3841 gctaagtgtc agatagaaaa aaatagaagg tttgctatgc gtagtttatt tgtgaaaaaa
     3901 tacactttat cgttttgtat agcacaaaaa tcattgctat gtaagcgttt ttacttacgg
     3961 taaaaatgat tatatacgta attcagatag ttatctatca tgaagtcata agcagcaaac
     4021 attcacaaaa ctaaggaggg gcttatggaa agtgtaaaga aacaagaacc atttcaactg
     4081 acgctatcga aaaaagaaga aaagctcaga agacgaaaaa aaataattgc aggaatcaaa
     4141 acgaataaat ttttatatgt gatgattctg ccgggagtcc tttatttctt gatttttagg
     4201 tatcttccta tggggggact cgttattgct tttcaagatt atcaaccatt tttaggaata
     4261 atgggaagtc cttgggtcgg tttcaaacat ttcattcgtt tgtttacaga accgacattc
     4321 tttacattat taagaaatac gttgatcttg ttcggattaa atatcttcgt gttttttccg
     4381 atgccaatca ttttgtcttt actattaaat gaagtaacga acaaacgatt gaaaaacggg
     4441 attcaaacga tcatttacat ccctcacttc atgtcttggg taatcattgt ttcgattact
     4501 tatgtatttt tgaatgttga cggaggggta ttgaatgaat tccttgcaac aatgggattt
     4561 gaaaaaatca gttttctaac atcccctgaa tggacacgca cagtatatat cggacaagtg
     4621 atctggaaag agttgggctg gtctacgatc atctaccttt cagcgataac tgcagtcgat
     4681 acacagttgt atgaagctgc agaaatggat ggagcaggac gcttacgcaa aacatggcat
     4741 gttacattgc cggcaattcg tccagtaatc attaccttgc tgattctaaa aatcggtcag
     4801 acattagact tagggttcga gcatatgtac ttgttattga attcattgaa tcgaagcgtt
     4861 gcagaaattt tcgacaccta tatttataca gctggattga agaatggaca gttaagtttc
     4921 agtacagcaa tgggattgtt taaaggggtc gtaggattgt ttatggtaat tttagctaat
     4981 aagattgcca aaaagctagg agaagacggc gtttactaag atagaaagag agggaaagat
     5041 atgctgcagt cattagataa aataaacgga aggctaaatc gtattttaca gttggtttat
     5101 ttgaattttc tgtggggcgg tttagtcatc gcaggtttgg gagtttttac tttcggtcct
     5161 gcatcgtatg cgatggttgc agtgattaga aaatggctgc gctcaaaaga aacgtttcct
     5221 ttgacgaaaa cgtacttcac atattttaaa gaaaactatc gtgaaacctt gctgatgagc
     5281 tggatatatg gcgccatcgg gattgtttta cttgtggatc tttctttcat acacaattgg
     5341 tatcttagga tcgctttgta tattgctgcg ttcatatatg tgctctcagc actctatatt
     5401 ttcccaatca tggctcatta tgattggaaa ggatattttt tcaaaatgaa aatggcattt
     5461 ttattcggat tctcacatct tcattactca ttagtgttga tattaagtat ggtagggatt
     5521 tatctgatga tcatccaaac aatgccaggt gttttaacct ttatcggtgg cagtctcttc
     5581 ttttccgcag tcacgtggac agcaaattct atattttgcc atttggaatg gaagaactca
     5641 tatacagaaa aaacagaaga aaacaaaaat attattaaaa cagtgaagga gaaaatttat
     5701 gaaaaaagca acgctcgtaa aattaggtct tagttttgca ggaattctag cacttgccgc
     5761 atgcggcagc agttcaagta acagttcttc agagacagaa gatggaaaag ctaaaatcac
     5821 ttggttaaat attttgcatc atgcttcacc gcctacagac acgattgtga acttgatcga
     5881 agaaaaaacc gatacggaac tgacattcaa ctggattcct gatgcttcaa aagaagaaag
     5941 attaacaact gcattggctt ctgatgaact aggagatatc gttacacttc aaattgcgat
     6001 gttgaaaaac tcttctgtcc gcaatgcgtt aaaatcaggc gtattctggg atgtatcaga
     6061 atatttaaaa gactataaaa atttaagtca aatttctgaa gatagaatcg aagctgcgag
     6121 tatcgacggt gccttgtacg gcgtaccgat acaaaaagac tacgctcgtg ctggcttagt
     6181 cattcgaaaa gactggttgg ataatctagg actagatgtt ccaactacca tggatgagct
     6241 ttatgaagta gctaaacaat ttacagaaaa cgatcctgat ggaaatggtg tggacgacac
     6301 agttggtttt ggcgatcgtg cgcctagtga tattcgttat accagcttta aacttttcac
     6361 ttcttatttt ggagcaccaa atggctgggc tgtagacgat aatggcaaat tcactccaga
     6421 atttgataca gaagagtata tgaaagcttt gaatttttca agagatctat ttgagaatgg
     6481 ctatttggca caagactttg cagttaccca aaagactgat cagcaagaac aatttgcaca
     6541 aggaagaact ggaatctaca ctggaatggt ggatatcaag aatctgaaga cgatgggtca
     6601 aggtattcaa gatgatatgg aattagtgcc tgtaaacaag atctcagatg gtgaaggcgg
     6661 cggttatcat gtttggtcag aaggaaatgg tattggcggc ttgctagcat ttcccaaatc
     6721 tcaagtaaaa gacgaagcac aattgaagaa attacttcaa tttgttgatg atcttttaga
     6781 agaagatgtg tataaattga tgactggcgg tatcgaagga acgcattatg aattgaatga
     6841 tgatggttct atcgaattta tcgatatgga tctttggcaa caagaggtgc aaccgttatc
     6901 aagctctcgt cctagtgaag tgacgttctc tttcaaagat acagaccctg aaaaagaatt
     6961 ctctaatcaa ttgatcaaag aaaatgaaga atttgctgta atggatcctt ctgtgccatt
     7021 ggattcaccg acgaacaatg aagtaggatc agaaattgaa aaaatcgtca ttgatgctac
     7081 ggtgcaatac atcatgggac agcttgacga agatggattc aaaaaagctg tagaagactg
     7141 gaaagcacaa ggaggcgatc gaatcacaca agagtacgag gaagcataca aagcagcggc
     7201 acagtaatat agacgattgg gacaaatact tcttggtatt tgtcccaaat cctattttaa
     7261 tttttaaagg agagcaaata atggaaaact ggtcaaagaa attagcaaaa agtatcatgg
     7321 aaagaacacc tcgcttatat gaagaaaagt ggtataaagg aaaatggtct tatgactatg
     7381 gcgtcgtact taaagggttc caattacttt gggaacgaac ccaagaaaaa atttattttg
     7441 atttcataaa agacaatatc gactatttcg tgcaagaaga tggaacgatc cgtggctaca
     7501 gcgttgaaga atacaatatc gaccatgtaa atacaggaaa gcttttcttt ttattgtaca
     7561 aagaaacagg cgaagaaaaa tataaaaaag cagcagaact cctttcccag cagttggcaa
     7621 accatccacg cacatccgaa ggagcttttt ggcataaaga aatttatcct tatcaaattt
     7681 ggttggatgg cttatatatg ggttctcctt tttatgctga atttatccag aattttagtg
     7741 aagaaaaaga ttaccaagat gttttgagac agtttgaaat cagttataaa catctattcg
     7801 atccaaaaac aaatttgctg attcatgcat gggatgaaaa gaaggtccaa ccatgggctg
     7861 atccaaaaac aggtctatcc catcattttt ggagccgatc gattggctgg tatttgatgg
     7921 ctgcagcaga tacctatgaa ttacttcatg aagaaacaga ttgttctttg ctgaaagaaa
     7981 ttctagaaaa gacactggct gcattacttc catatcaaga agaaaacagc gggacatggt
     8041 atcaagtaac aacagaaaca aaacggaaag gaaattatct ggaagcttcc ggcagtagta
     8101 tgtttgttta cgcaatggct aaaggaatac gtttgggact gctgaacaaa gaagaatggc
     8161 ttccacaagt gaagaaatca catcaaggat tacttgatga atttgttctg gaaacaaaag
     8221 aaggctggct gaacttgaat aaaaactgtc aagtagcagg gttaggcgga gcagatcagc
     8281 gagatggttc atatgcttac tacatcagtg aaccaattat ttgtaatgat caaaaaggcg
     8341 ttggtgcatt cttgcaagca ttgattgaag tggaggcatt ataaatatgt atgatatttt
     8401 gacatttggc gcatcaattg atgaattgaa tactgaagca atacaacaag ccattgatgc
     8461 tgcagcaagt gacggaggcg ggaccgtagt tgttccagca ggtgaatttc taacaggagc
     8521 tttattttta aaaagcaatg tcgaacttca tttaagcgca ggagcggttc tgaaattctc
     8581 tgacgatcca aaagattatc ctgttgttca ttcacgttgg gaaggagtcc atcgaaaagt
     8641 ttatgcttcc tgtatctatg ctcaaaatgt agagaacatc tctgtcactg gtttcggtac
     8701 attagatgga aatggaaaaa aatggtggca tacttttaga aatgaaccag ataatctcgc
     8761 ttatcctcgt cctaaattga tgagtttcca taactgtcat cgaattactg tgaaagatat
     8821 taaattgatc cagtcaccaa gttggacgat caacccgatt ctttgcagta atgcaacgtt
     8881 tgataacttg acgatcttga acccagcgga ttcaccaaat acagacggaa ttgatccaga
     8941 atcatgcaaa aatgtgcgta ttagtaattg ccatattgac gtgggagatg actgtattgc
     9001 aatcaaggca gggactgaag atacgtacga aaggatcgct tgcgaaaata tcacgatcac
     9061 aaattgcacg atggtccatg gacatggcgg tgtcgtatta ggaagtgaaa tgagtggtag
     9121 tattcgtaat attaccattt caaattgtat cttccaagaa actgatcgag gaatacgatt
     9181 gaaatctaga cgaggtcgag gcgggatcgt tgaagatatt cgagtatcca atatagtcat
     9241 ggataatgtg atgtgtccat ttatactgaa tctttattat ttctgtggtc cgcgagggaa
     9301 agaaccgtat gtatgggaaa aaaaggctta tccgatagat gaacggacac cagcattcag
     9361 aaggatccat ttttcgaata tcacggcaag gaatgtccat gcttctgcag gttttattta
     9421 tggattagcg gaacaattca tacaagaaat cacatttgac aatatcgatg tctcgatggc
     9481 ccaagatgca gttcccggtg ttcctgctat gatgacagga atagaggaaa tgagcagaca
     9541 aggattttat attggctttg cgggtaaggt ttcctttaac cagatgtcga ttgaaaatca
     9601 cgagggacca gcatttctat tagaaaatac taaagaaatc agtattgaga aattccaatc
     9661 aaatcaatca gaaaatatgg atgaactggt tgttcgtaaa aactttgctt atgcagttga
     9721 atgatttttt aggtgctcca tctagcagac atacccctag tggaaaacga ttataccgag
     9781 aaaaacaaaa cggttttatg cttgaatact atcaattgta cttaaatgaa ttggaaacag
     9841 taccagccct ctatgcttat ccagaaaaag aaggaccgtt tccgccggtc ctttacctgc
     9901 attctcacgg cggagatttt tcagtcggga aaagtgaatt gatccatggt gctccttact
     9961 tagcttctcc atcttttgca gaagtactga caggaatggg atatgctgtt tggtcgattg
    10021 atgcttgggg atttgaagaa cgcggaggta tttctgaaag cgagttgttc aaacaatttt
    10081 tattaacagg aaaaacgtta tggggaatgc gtatttatga tgtgatcagc ttgatcgatt
    10141 atttggagac aagggaagac acagatactc aacggctcgc aacgattggt ctttctatgg
    10201 gaggcttatt aagctggtgg gtgacagcat tggattctcg tgtcaaagtt tgtattgatc
    10261 tagcggcaca ggtggacatc gaaatacttt tgctacatcg ccgcctagat catcatgggt
    10321 tttattacta tgtgccaaat ctattgacag cttatacaac gttagcaatc caagaaaaaa
    10381 tcgctccgcg ccctcgtctt agcttagttg gaaaaaacga tacgatgtgc ttggacgaag
    10441 gcgtactgaa gctgagaaaa gggctggaca aaaagtatca atcaatgggt agtccagaaa
    10501 acttttcttc tttttcttta acaggcggtc atatggaaac acaagaaatg agaaatattt
    10561 ggaaacaatt cataagggag catttatgag gatgaaagac aaactgataa ttggaaagaa
    10621 tgctatagat accccgacgg attttgaaac tatccaagaa ggaatcgatt atttggcagg
    10681 cctgccggat agccatccaa aggtcttgat agtaaaagaa ggaagatatg atgaatatat
    10741 agaaagcaga ttatccaatt ttaaacttgt gggagcagga acagtagtta tctccggtaa
    10801 tcgttttgca aaacaggagt atcttgacgg tagccagtta gggactttcc gaagtgctac
    10861 ttttttctta gaaggatcga atgtcgtatt agaaaatctt cacatcgaaa atacagcagg
    10921 tccaggagaa aaggtaggac aggctgttgc tttattcaat tcaggagatc gcgtatcttt
    10981 aaaaaactgt cgactatctg gttatcagga taccttatgc acgggtccgt tgcctgattt
    11041 gcaaaaagat ggtactcctt tttgtacacc ggcttctgcc aatcctgagt attgtcgtca
    11101 gacctatgaa tattgctgga ttgaaggaac ggtggatttc atatttggtg gggcagatgc
    11161 cgtttttcat cagtgtcata tccatagctt agccagtaaa aatcctggat acatcacagc
    11221 agcatctacg ccgaaaaatc aaaaaacagg gtatcgtttt gaaagctgta ccttgactgc
    11281 agaacctgat actagaaacg tctatttagg aagaccgtgg cgtccgtatg caaatgtctt
    11341 atttgaagcg tgtaagatag gtgggcatgt tcacccaact ggttgggata attgggatga
    11401 caagaaaaat gaacagactg tcaaattctc tgaacgtaat aacaattatg aaacatcgat
    11461 caaaagacct gaatggatac atttagaaaa gcagggggtt cagaaatgaa aagaaaatgg
    11521 gaactattgc taggaatggt cggtggcagt ctatctttga ttttctttgg cgggttagca
    11581 gttactttaa gtaatatgtc tgcgtcagag tttaaaaaaa gttatcagtc gcttgctgta
    11641 gatcattcaa ctttgtcttt agaaaataca tttggattat tacaggacat gactggtctg
    11701 tttgccgttg tgttgtttat ttcacttgct tttttagcag tagcattgtt tttgactgct
    11761 aaaggaaaat acttaacaac tgccactggt ttgtatttta taacaggatt catattgttg
    11821 attggcactc agtttattgc gtttcctttt gcattttttt atttcgcagc tggggcattc
    11881 agtttatatc gagtaagaat gagaaaagga gcctgaatca tgcagactat caaagtgaaa
    11941 aatcctattt taagaggatt caatccagat cctaatatat taagagtgaa agagcggtat
    12001 tacattattg tctcctcttt tgaatggctt ccaggaatcc gagtatatga gtcgaaaaat
    12061 ttagtcaact ggtcacatac aacggatatt ttgaccactc aagtatcttt gcaaggaaat
    12121 cctacaaatg gaagtatatg ggcgccacaa atcagttatg ctgatggatt gttttatctt
    12181 gtttacacag atgtcaaaag tgcaggccgt ccgtttaaag atgtccacaa ttatttgatc
    12241 actgcatctg agatcacagg tccttggtcc gaaccaattt atttaaatag taacggcttt
    12301 gatccttctt tgttccatga tcttgaaacg ggaaaaaaat ggcttgtcaa tgaactatgg
    12361 gattatcgtc tggacacacc gaataaatcc agcggtatcg tattacaaga atatgatgca
    12421 gagaagaaga agcttgatgg tcccatctat aaaatatttg caggtacaga attagcgaaa
    12481 acggaggcac cacatttgta ttttcgtaac gggtattatt atttgatgac ggcagaaggc
    12541 ggtacaggaa gaggacattc agttacagtt tgccgagcaa agaatattac tggtccttac
    12601 gagcttgatc ctggttttcc tatgctgaca gctagtgaca aacctgcttc tacattgcaa
    12661 tgtacaggac atgggagctt agtacaaacc ccgtcaggaa gatggtacat gacatatctt
    12721 tgtaccagac cattaaacgg tgcggctatt ttgggaagag aaaccgctat tcaagaagtg
    12781 tattggacgt ccgacgattg gttaagactt aaagatagcc agacaactcc gatggaagaa
    12841 ataatgattg agacaaatga agaggtacaa caagaaaaag atcatcagtt tatggataca
    12901 tttgaaggag agctaaagaa gaactggaat gccagacgca ttttgccaaa tgctgattgg
    12961 tgtgatacaa aatgtagacc aggtttttta cgtctgattt ccggtgaatc tcttcaatct
    13021 accttcgaac atcatttgtt agctattcgg caaacggatt ttacttttga tgcggagaca
    13081 gcatttgaat acatgcctaa taattttaat cagatggctg gactggtact gtacttaaat
    13141 gaatccaatt atatttattg ttatgttact tggaatgagg acaagggaaa atgcctacgt
    13201 atgatagaat cagaaaacgg agtagcacaa atagaagatt ggacgattcc actcacagaa
    13261 aagaagactt ttttgaaagt cactgtaaag aaagagacag cacagtttca tttgaaagac
    13321 gtatcaactg aagaatggaa gaaagtaggt ccgcaaaaaa acatgtgtat actctcagga
    13381 gggtttactg gaaattttat cgggatagca gtgcatgatc taaataaaaa aagaggaagt
    13441 tatgctgatt ttgagttttt caagtatgat ggaaaagatc atctgtaatt taattcttta
    13501 ataaatgagg ttcactaact tattttcact agttagtgaa tttttttgtt tgtatatatt
    13561 gttatgtaa
//
LOCUS       DAZBWR010000080        13084 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000080 DAZBWR010000000
VERSION     DAZBWR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.082"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACHGT5_001999"
     CDS             complement(78..2198)
                     /locus_tag="ACHGT5_001999"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN7217972.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACHGT5_002000"
     CDS             2428..3606
                     /locus_tag="ACHGT5_002000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN7217973.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACHGT5_002001"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACHGT5_002001"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN7217974.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHGT5_002002"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHGT5_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN7217975.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACHGT5_002003"
     CDS             complement(4959..6212)
                     /locus_tag="ACHGT5_002003"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN7217976.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHGT5_002004"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHGT5_002004"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN7217977.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACHGT5_002005"
     CDS             7789..9135
                     /locus_tag="ACHGT5_002005"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN7217978.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACHGT5_002006"
     CDS             complement(9443..9829)
                     /locus_tag="ACHGT5_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN7217979.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACHGT5_002007"
     CDS             complement(10097..10246)
                     /locus_tag="ACHGT5_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217980.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACHGT5_002008"
     CDS             10708..10920
                     /locus_tag="ACHGT5_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217981.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACHGT5_002009"
     CDS             complement(11198..12997)
                     /locus_tag="ACHGT5_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN7217982.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DAZBWR010000081        12738 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000081 DAZBWR010000000
VERSION     DAZBWR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.083"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHGT5_002010"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHGT5_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN7217983.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHGT5_002011"
     CDS             complement(323..892)
                     /locus_tag="ACHGT5_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN7217984.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHGT5_002012"
     CDS             1078..1980
                     /locus_tag="ACHGT5_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN7217985.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHGT5_002013"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHGT5_002013"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN7217986.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHGT5_002014"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHGT5_002014"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN7217987.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHGT5_002015"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHGT5_002015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN7217988.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHGT5_002016"
     CDS             8548..8733
                     /locus_tag="ACHGT5_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN7217989.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHGT5_002017"
     CDS             complement(8795..9991)
                     /locus_tag="ACHGT5_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN7217990.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHGT5_002018"
     CDS             complement(10161..11597)
                     /locus_tag="ACHGT5_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN7217991.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHGT5_002019"
     CDS             11890..12699
                     /locus_tag="ACHGT5_002019"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7217992.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatt
//
LOCUS       DAZBWR010000082        12625 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000082 DAZBWR010000000
VERSION     DAZBWR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.085"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHGT5_002020"
     CDS             complement(287..649)
                     /locus_tag="ACHGT5_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7217993.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHGT5_002021"
     CDS             complement(723..1103)
                     /locus_tag="ACHGT5_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN7217994.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHGT5_002022"
     CDS             1185..2360
                     /locus_tag="ACHGT5_002022"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN7217995.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACHGT5_002023"
     CDS             complement(2437..3021)
                     /locus_tag="ACHGT5_002023"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN7217996.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACHGT5_002024"
     CDS             3222..4220
                     /locus_tag="ACHGT5_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN7217997.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACHGT5_002025"
     CDS             4332..4841
                     /locus_tag="ACHGT5_002025"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN7217998.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACHGT5_002026"
     CDS             complement(4871..5530)
                     /locus_tag="ACHGT5_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN7217999.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACHGT5_002027"
     CDS             complement(5532..6296)
                     /locus_tag="ACHGT5_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN7218000.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACHGT5_002028"
     CDS             complement(6304..6981)
                     /locus_tag="ACHGT5_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN7218001.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACHGT5_002029"
     CDS             complement(7018..8403)
                     /locus_tag="ACHGT5_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN7218002.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACHGT5_002030"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHGT5_002030"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHGT5_002031"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHGT5_002031"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN7218003.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ACHGT5_002032"
     CDS             9342..10199
                     /locus_tag="ACHGT5_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218004.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ACHGT5_002033"
     CDS             complement(10280..10615)
                     /locus_tag="ACHGT5_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218005.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ACHGT5_002034"
     CDS             complement(10682..12052)
                     /locus_tag="ACHGT5_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN7218006.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..>12625)
                     /locus_tag="ACHGT5_002035"
     CDS             complement(12130..>12625)
                     /locus_tag="ACHGT5_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7218007.1"
                     /translation="FKSMVYVEEYGMKSITARELAEFCGCSTYPIYTHFKSISGLKEK
                     ILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDLDMDSLFKRPFF
                     SYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNEVTDEYLENILK
                     EQSP"
BASE COUNT         3755 a         2556 c         2115 g         4199 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaac
//
LOCUS       DAZBWR010000083        12458 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000083 DAZBWR010000000
VERSION     DAZBWR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.086"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..457)
                     /locus_tag="ACHGT5_002036"
     CDS             complement(143..457)
                     /locus_tag="ACHGT5_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218008.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            743..982
                     /locus_tag="ACHGT5_002037"
     CDS             743..982
                     /locus_tag="ACHGT5_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218009.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1344..1604)
                     /locus_tag="ACHGT5_002038"
     CDS             complement(1344..1604)
                     /locus_tag="ACHGT5_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218010.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1789..2286)
                     /locus_tag="ACHGT5_002039"
     CDS             complement(1789..2286)
                     /locus_tag="ACHGT5_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN7218011.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACHGT5_002040"
     CDS             complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACHGT5_002040"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN7218012.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACHGT5_002041"
     CDS             complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACHGT5_002041"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN7218013.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5008..5670)
                     /locus_tag="ACHGT5_002042"
     CDS             complement(5008..5670)
                     /locus_tag="ACHGT5_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN7218014.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5680..6495)
                     /locus_tag="ACHGT5_002043"
     CDS             complement(5680..6495)
                     /locus_tag="ACHGT5_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7218015.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6757..7200)
                     /locus_tag="ACHGT5_002044"
     CDS             complement(6757..7200)
                     /locus_tag="ACHGT5_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFN7218016.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7334..7672)
                     /locus_tag="ACHGT5_002045"
     CDS             complement(7334..7672)
                     /locus_tag="ACHGT5_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN7218017.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7660..8037)
                     /locus_tag="ACHGT5_002046"
     CDS             complement(7660..8037)
                     /locus_tag="ACHGT5_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN7218018.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8261..9454
                     /locus_tag="ACHGT5_002047"
     CDS             8261..9454
                     /locus_tag="ACHGT5_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7218019.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9620..10042)
                     /locus_tag="ACHGT5_002048"
     CDS             complement(9620..10042)
                     /locus_tag="ACHGT5_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218020.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10413..11087
                     /locus_tag="ACHGT5_002049"
     CDS             10413..11087
                     /locus_tag="ACHGT5_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218021.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(11249..12421)
                     /locus_tag="ACHGT5_002050"
     CDS             complement(11249..12421)
                     /locus_tag="ACHGT5_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN7218022.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         3826 a         2449 c         2046 g         4137 t
ORIGIN      
        1 taaactctag agaaattaat tcatcctaac ataaaataat tgttcactca aaagtttttc
       61 tagaacaatt aaaacagcag gcttcacatt cgctatacat ttgtaagaag taataattcc
      121 tgtactaaaa ggcataattg ctttaattat tttttgactt ctcttcaaac acaagaagta
      181 gttgtgtaat aattgcagaa ataaggattc caataataga tataaaattt gacatggaaa
      241 cgtctataac attaaaagaa taaagtgttg aagaaagtaa actgattgaa ataacccctc
      301 cgtaaaaaat agtctgtgaa tatacccact ttttttcaga agacgtactt cgtttggttc
      361 taaaacctat ccctgcattt ctactcttat caaaaaacca aatatttaga agtaataata
      421 ttgctattat taagataaaa ttactgtaaa aatccaaaag ctatcctcct agcaactatt
      481 taaataccca aaaaaattac ctctagataa tatcatacct acgttttcac ttataaattt
      541 tttcataaaa aaagcgttta gaggcataat cgttaataca gatggtaacc aatgtatctg
      601 gtttgaactg ggatatcacc ctctccatat atgtattact aagactaata gatactataa
      661 aagacttgat agttttgcat tgattttgcc taatactgag cataggtgta tgctaaacga
      721 tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat caaatttatt
      781 gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata cgcaaatttc
      841 gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag aaacttccga
      901 tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac gtctccttta
      961 tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga tgatagtttc
     1021 gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa gcctgagcta
     1081 aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga tctttttaaa
     1141 actactagtt tgtctatccg aaaataggac agttctggca gtataggttt ttatcgagtt
     1201 tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga ataaatcaaa
     1261 aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt cttctcgctt
     1321 ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt gttgctatag
     1381 ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc cactactttt
     1441 ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac gcagatcaca
     1501 acacaatttt ctaatttact gattacttct ccaatcactt tttgtttcaa gccaataggt
     1561 tggcaaatat atttttttcc taaaatgata gtttcgttat tcatatttca tccatcctta
     1621 atgttttttc attatttaaa atgataataa cattaaaata tgattatgaa aatcaataac
     1681 acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag aaatgcccac tgtctttccg
     1741 gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt agttattaat
     1801 aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt tttctagaaa
     1861 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
     1921 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
     1981 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
     2041 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
     2101 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
     2161 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
     2221 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
     2281 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
     2341 taaacgctag gttttctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
     2401 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
     2461 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
     2521 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
     2581 tgtatcatcg ataaaatgca ggtgaaatcc cgctgcagca actccttgga ataattcagg
     2641 cgtaaagaac ccgactaatg ttccttggac attgctttgt tggaattctg gttgagactc
     2701 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
     2761 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
     2821 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     2881 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     2941 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     3001 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     3061 tccatccatc aagccagcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     3121 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     3181 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     3241 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     3301 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     3361 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     3421 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     3481 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     3541 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     3601 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     3661 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     3721 tccttttgat tgaacaatgt cccgctccgt taattctgct tgaagagatg ctaaatattc
     3781 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     3841 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     3901 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     3961 gattggatca tagccaatag caataactag atcacttcgt ttcaaaagca tatctcctgg
     4021 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     4081 tccagctgct tggaatgtct ctacaacagg caatgctgtc tttccaacta attgcctgat
     4141 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     4201 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     4261 atcgctcaat ggcttgatga cattcccttg gacaggtgca tccactacat cttgcgggat
     4321 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     4381 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     4441 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     4501 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     4561 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     4621 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     4681 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     4741 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcgtc
     4801 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     4861 aattgataca gtttctaaac ctgtttcatt caaaagtata gcacgtttta tttcatttgt
     4921 ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa actgtcattc
     4981 ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca atttacgatt
     5041 gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc attggggctt
     5101 tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca
     5161 ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg acgctggatt
     5221 tctcacctga aaataccagt agttgatgct aaaggaaact gataaacaag caagtaaaac
     5281 aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac aatcaaaaat
     5341 cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta gccaaaattg
     5401 gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt ttttcttttg ctaattcttc
     5461 tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa aaataaagga
     5521 aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca agatattgac
     5581 acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga attccatata
     5641 aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca ttcctgatga
     5701 agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa caggaagaac
     5761 agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc caataaacat
     5821 tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga atggccccat
     5881 ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt
     5941 aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga tccgcgcact
     6001 ctcacataaa gaatctggga tactactgta gtattgcttc aacaagaaca caccaaaagc
     6061 attgaacgct tgtaatagca ccaacgacca caacgtatat gttaaaccca cttttctcat
     6121 cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc cgatatatgc
     6181 taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag cagcaaaact
     6241 agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa
     6301 tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc aagttttagg
     6361 gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag cagaaaccat
     6421 ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca tacccaacca
     6481 ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat ttcgaacacc
     6541 tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac ggttattata
     6601 gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt tatgtaaatc
     6661 tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc atacgtctcg
     6721 cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag cttctttgaa
     6781 acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc agactagcat
     6841 gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg aaagatgact
     6901 tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg caaatacatt
     6961 gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc ctaaccagtc
     7021 agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc
     7081 ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga gcaatcccca
     7141 tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg gatctagcaa
     7201 aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac ttgttccgct
     7261 gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta atagtgccaa
     7321 tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga ataattgttt
     7381 ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt cttctgctcg
     7441 gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg cgtacaaaca
     7501 gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa
     7561 gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc caaaaatatt
     7621 ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac attccatttt
     7681 atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt ccaaacattc
     7741 ttgcatttca gcaatttttt tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg
     7801 ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca aagactcgat
     7861 cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt cttcttctgt
     7921 ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga ttcgttcata
     7981 atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga tattcatctg
     8041 atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat tctacaactt
     8101 taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta tctatatcgg
     8161 ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt tggatatact
     8221 tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa
     8281 gttattatta tcgacatcgg cgggaattgc tttagagaat atggatatta tgtttttagc
     8341 tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag cgggtttcat
     8401 ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca tcatagctga
     8461 taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga ttgcttcttt
     8521 attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta tcgcaggtgt
     8581 aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa
     8641 acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg cggctttggc
     8701 tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt atgtcgtagg
     8761 tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt
     8821 caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg aatcaaaaca
     8881 tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg ccggctattt
     8941 tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt cggtttctgg
     9001 gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga tgacttttgg
     9061 cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa tttgttcatc
     9121 cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag cagccgctgt
     9181 gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga ttagtagttt
     9241 gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg gacgagctgt
     9301 tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt taactgccgt
     9361 aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa ctcttactgg
     9421 tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa
     9481 gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa aacatgagac
     9541 actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca actgctaatt
     9601 tccttctatc tggcggctac tattctttga cgtccgtcat ttcaaagtac tttactaata
     9661 acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca taatcttcca
     9721 aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat gattttctgt
     9781 agaaatgaag ataaataatg tagattggga agttctcctg cttttccata gaaaagcgcc
     9841 gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat tcttctaaat
     9901 ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt agttcttcta
     9961 ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc ttttgagaaa
    10021 cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta tactacatac
    10081 aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca taaacctgtt
    10141 attatttctc tttttttcct aagtattcta ttgatatgaa tcatttaatc gtttattata
    10201 taaatgtcaa taatctaata ataaaataac gtacatattt tattattgca tacttaataa
    10261 cagaactata gcaaaaaatc ccgtgtatca aaacgggatt agattaagtt tcctcaatca
    10321 ctattcataa cacaattatt ataacaagta agtaaacaat tgaaaaggac atttattgaa
    10381 aacggttgtt ttttacttaa ggaggaaatt tagtgaaaaa attagttgca ttgtttttgg
    10441 tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc tactactcaa agcacaagtt
    10501 cagctcaggc ttccagcagc agtaccacga tctccgaaag tacggcagat tcatcgaaag
    10561 aacaagaaca aattgaaaag aaaaaagaag aagagaaaca aaaagcggaa aaagctgcac
    10621 tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca aatcctacaa
    10681 aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac aaacaagagt
    10741 tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca gaagaagaac
    10801 ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa gaagcaatcg
    10861 cacagcaaca agcagaacag gcgcgacagc aagcagcagc tgaacaggcc tctcagcagg
    10921 cacaagccgc agctcctgag caaaataatg aacaaactgt ctatgtcaca ccgaccggtt
    10981 caaaatacca tacacataaa tgtggaaatg gtacgtatac ccctgccact ttaagcgaag
    11041 ctcaagctcg tggtttaacc ccttgctcca aatgttatgg aaactaattc aaaagttata
    11101 ctataataat aaaattcttc tgaaacataa aaaaatagaa gctggcgttc cagatagtaa
    11161 accaaaaata gcagtttaca cgtccatttc gtcagcttct ctcttttttc atttagtctg
    11221 ttccctctgc ttttttggac atcttcattc attctttctc aatcaaatag tacacctctt
    11281 tttcttttcg cagtttatat cttccttttt tcaatttcat tgaaaactgt ttcgaatgtt
    11341 tatgttttac atggatttta agcaggagct cctcatgttg caagcgatag ttcctgcctt
    11401 ttaaatcaga aaatctcact agtccgtatt catcagaagc tttcacaaat ggaacttttt
    11461 ctctagtaat atagtgtttg ccgttgagcg tttttacaga aaagaccatt ccttcgattg
    11521 gtcgtccgtt tgtgttcttt acataaaaag ataaaggata gtttcgctta gcgatggcca
    11581 aagaatctaa ataagcgatg agtagccaaa ctaagccgat tgatccaagt agtataccga
    11641 taaaccaaat aaaaaatttc cattttttcc attgatcaat tccttggatc gctttttttg
    11701 cctcttttgc ttgataagga atacgatgcc ccgtcacaag cagtcgatgt gtattgacca
    11761 tataaggagt acaggtaagc aaagtcacga gatctctgcc tttttggata cccaaagaat
    11821 ctacttcatc aggtaagacc acttggattt tttctacttg atacgccaat gtctttccat
    11881 tgatctggat gaaaaactgg tctccttttt tcaacttagg caaatctgta aataattttg
    11941 cttctgggag tccacgatgc ccagaaagaa cggcatgggt gtttttcccg ccagtcggat
    12001 aagaactttc ttccaataat gacgttcctt tttgcaaaaa tatttccgtt gtctgatcga
    12061 atataggtaa gctgacattg atttttggga tttcgatgac tccaagcata tgttccataa
    12121 aaaaggcatt actaggtaaa tcttttaatg cttttgcatc aactgcttgg ttgaaactgc
    12181 ctatccctgg tgatacgttt tgctctgcca actgttggtt tttctttgtc attttttcct
    12241 gctgtttttt tatttcctct gaattcttat tcgatgcttg cttttgataa tggatgatca
    12301 tttgctgggc aagaaaatca ttcagtgaat cttctacaaa aggataagaa aagatcccga
    12361 tcccaacgac taaaatgatt gccatgaaga tgtccagtat tcttattctg cttttattca
    12421 tcatgtccac ctattttcta atatgtatat gaagaggt
//
LOCUS       DAZBWR010000084        12222 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000084 DAZBWR010000000
VERSION     DAZBWR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.087"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHGT5_002051"
                     /pseudo
     CDS             1..369
                     /locus_tag="ACHGT5_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(495..1574)
                     /locus_tag="ACHGT5_002052"
     CDS             complement(495..1574)
                     /locus_tag="ACHGT5_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN7218023.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1677..2117)
                     /locus_tag="ACHGT5_002053"
     CDS             complement(1677..2117)
                     /locus_tag="ACHGT5_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN7218024.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2174..2584)
                     /locus_tag="ACHGT5_002054"
     CDS             complement(2174..2584)
                     /locus_tag="ACHGT5_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN7218025.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2764..3198)
                     /locus_tag="ACHGT5_002055"
     CDS             complement(2764..3198)
                     /locus_tag="ACHGT5_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7218026.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3298..4146)
                     /locus_tag="ACHGT5_002056"
     CDS             complement(3298..4146)
                     /locus_tag="ACHGT5_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN7218027.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4535..6301
                     /locus_tag="ACHGT5_002057"
     CDS             4535..6301
                     /locus_tag="ACHGT5_002057"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN7218028.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6403..7662
                     /locus_tag="ACHGT5_002058"
     CDS             6403..7662
                     /locus_tag="ACHGT5_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN7218029.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7809..9110
                     /locus_tag="ACHGT5_002059"
     CDS             7809..9110
                     /locus_tag="ACHGT5_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7218030.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9113..9964
                     /locus_tag="ACHGT5_002060"
     CDS             9113..9964
                     /locus_tag="ACHGT5_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN7218031.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9977..10792
                     /locus_tag="ACHGT5_002061"
     CDS             9977..10792
                     /locus_tag="ACHGT5_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN7218032.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10943..12109
                     /locus_tag="ACHGT5_002062"
     CDS             10943..12109
                     /locus_tag="ACHGT5_002062"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7218033.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4126 a         2171 c         2284 g         3641 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
      481 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
      541 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
      601 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
      661 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
      721 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
      781 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
      841 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
      901 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
      961 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     1021 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     1081 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     1141 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     1201 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     1261 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     1321 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     1381 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     1441 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     1501 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     1561 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     1621 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     1681 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     1741 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     1801 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     1861 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     1921 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     1981 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     2041 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     2101 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     2161 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     2221 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     2281 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     2341 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     2401 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     2461 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     2521 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     2581 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     2641 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     2701 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     2761 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     2821 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     2881 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     2941 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3001 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     3061 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     3121 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     3181 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     3241 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     3301 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     3361 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     3421 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     3481 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     3541 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     3601 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     3661 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     3721 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     3781 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     3841 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     3901 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     3961 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     4021 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4081 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     4141 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     4201 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca
     4261 ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg
     4321 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa
     4381 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
     4441 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt
     4501 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
     4561 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
     4621 taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac
     4681 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
     4741 ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt
     4801 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
     4861 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
     4921 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
     4981 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
     5041 ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac
     5101 ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
     5161 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
     5221 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
     5281 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
     5341 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     5401 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     5461 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     5521 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     5581 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     5641 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     5701 tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     5761 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     5821 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     5881 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     5941 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     6001 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     6061 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg
     6121 tgaagataag ctctatttca cacggaaact agagggacag atcatctatg tttactttaa
     6181 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     6241 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     6301 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     6361 taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg
     6421 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     6481 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     6541 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     6601 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     6661 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     6721 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     6781 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     6841 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     6901 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     6961 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     7021 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     7081 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     7141 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     7201 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     7261 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     7321 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     7381 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     7441 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     7501 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     7561 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     7621 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     7681 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     7741 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     7801 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     7861 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     7921 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     7981 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     8041 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     8101 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     8161 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     8221 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     8281 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     8341 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     8401 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     8461 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     8521 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     8581 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     8641 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     8701 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     8761 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     8821 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     8881 atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg
     8941 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     9001 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     9061 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     9121 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     9181 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     9241 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     9301 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     9361 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     9421 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     9481 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     9541 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     9601 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     9661 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     9721 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     9781 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     9841 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     9901 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     9961 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
    10021 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
    10081 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
    10141 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
    10201 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
    10261 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
    10321 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
    10381 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
    10441 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
    10501 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
    10561 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
    10621 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
    10681 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
    10741 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
    10801 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
    10861 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
    10921 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
    10981 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
    11041 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
    11101 tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
    11161 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
    11221 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
    11281 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
    11341 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
    11401 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
    11461 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
    11521 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
    11581 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
    11641 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
    11701 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
    11761 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
    11821 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
    11881 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
    11941 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
    12001 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
    12061 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
    12121 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
    12181 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tt
//
LOCUS       DAZBWR010000085        12192 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000085 DAZBWR010000000
VERSION     DAZBWR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.088"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..557)
                     /gene="frr"
                     /locus_tag="ACHGT5_002063"
     CDS             complement(<1..557)
                     /gene="frr"
                     /locus_tag="ACHGT5_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN7218034.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(560..1282)
                     /gene="pyrH"
                     /locus_tag="ACHGT5_002064"
     CDS             complement(560..1282)
                     /gene="pyrH"
                     /locus_tag="ACHGT5_002064"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN7218035.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1418..2299)
                     /gene="tsf"
                     /locus_tag="ACHGT5_002065"
     CDS             complement(1418..2299)
                     /gene="tsf"
                     /locus_tag="ACHGT5_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN7218036.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2398..3180)
                     /gene="rpsB"
                     /locus_tag="ACHGT5_002066"
     CDS             complement(2398..3180)
                     /gene="rpsB"
                     /locus_tag="ACHGT5_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN7218037.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3539..4018
                     /locus_tag="ACHGT5_002067"
     CDS             3539..4018
                     /locus_tag="ACHGT5_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN7218038.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4235..5326
                     /gene="phnW"
                     /locus_tag="ACHGT5_002068"
     CDS             4235..5326
                     /gene="phnW"
                     /locus_tag="ACHGT5_002068"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFN7218039.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5319..5447
                     /locus_tag="ACHGT5_002069"
     CDS             5319..5447
                     /locus_tag="ACHGT5_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN7218040.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5451..5996
                     /locus_tag="ACHGT5_002070"
     CDS             5451..5996
                     /locus_tag="ACHGT5_002070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFN7218041.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6450..8141)
                     /gene="argS"
                     /locus_tag="ACHGT5_002071"
     CDS             complement(6450..8141)
                     /gene="argS"
                     /locus_tag="ACHGT5_002071"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN7218042.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8561..9508)
                     /gene="arcC"
                     /locus_tag="ACHGT5_002072"
     CDS             complement(8561..9508)
                     /gene="arcC"
                     /locus_tag="ACHGT5_002072"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN7218043.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9623..10642)
                     /gene="argF"
                     /locus_tag="ACHGT5_002073"
     CDS             complement(9623..10642)
                     /gene="argF"
                     /locus_tag="ACHGT5_002073"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN7218044.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10733..11962)
                     /gene="arcA"
                     /locus_tag="ACHGT5_002074"
     CDS             complement(10733..11962)
                     /gene="arcA"
                     /locus_tag="ACHGT5_002074"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN7218045.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3343 a         2524 c         2195 g         4130 t
ORIGIN      
        1 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
       61 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      121 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      181 atttttgcat tttctgcttc ttttttaaca tctttagcta attcttttct gcgttcttct
      241 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      301 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      361 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      421 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      481 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      541 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      601 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      661 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      721 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      781 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
      841 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
      901 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
      961 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1021 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1081 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1141 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1201 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1261 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1321 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1381 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1441 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1501 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1561 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1621 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1681 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1741 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1801 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     1861 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     1921 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     1981 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2041 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2101 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2161 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2221 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2281 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2341 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2401 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2461 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2521 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2581 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2641 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2701 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2761 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     2821 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     2881 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     2941 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3001 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3061 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3121 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3181 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3241 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3301 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3361 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3421 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3481 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3541 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3601 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3661 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3721 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3781 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     3841 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     3901 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     3961 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4021 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4081 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4141 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4201 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4261 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4321 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4381 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4441 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4501 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4561 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4621 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4681 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4741 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4801 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     4861 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     4921 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     4981 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5041 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5101 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5161 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5221 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5281 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5341 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5401 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5461 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5521 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5581 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5641 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5701 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5761 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     5821 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     5881 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     5941 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6001 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6061 gttaacagaa tacattttgt acgctgacat gaatccagaa tgacttttat acaaaataag
     6121 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6181 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6241 cgaaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tgcaaacaaa
     6301 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6361 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6421 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6481 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6541 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6601 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6661 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6721 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6781 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     6841 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     6901 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     6961 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7021 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca
     7081 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7141 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7201 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7261 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7321 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7381 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7441 tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag acgtttgaac
     7501 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggacg
     7561 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7621 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7681 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7741 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7801 gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac tactgtttgc
     7861 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     7921 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     7981 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8041 gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc atctttgaca
     8101 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8161 ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8221 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8281 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8341 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8401 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8461 gctagtcgtt tcataaaaaa acaatccaga tggcctaaag gagaagaaat accacctgga
     8521 tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct acgataatcg
     8581 ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg attgccttag
     8641 cgtttggttt tgcttcaaca aatgcaatag ctgcttctac tttcggaagc atgcttcctg
     8701 gagcgaattg cttttcatca atgtattgtt tcatttctga aacagtaaca gtttctaatt
     8761 tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc aatacgatca
     8821 gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta tcgattacgg
     8881 cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg ccaccaacag
     8941 agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga
     9001 tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct ttgaatgtca
     9061 cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat ggaccgactg
     9121 gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga gtgactaaag
     9181 aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggcg ttttgtaacc
     9241 agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt gccggtgttt
     9301 tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga ccatttccgt
     9361 gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt gctgtttcca
     9421 ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagatggca ttgcccccta
     9481 aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt ctaccgaatt
     9541 ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga ctcgtttttt
     9601 cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct aatgttgctg
     9661 ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga cgagcatatt
     9721 tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt tctttcattt
     9781 gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag aagatcaaac
     9841 gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt ttcaacaatt
     9901 tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca gaataaagga
     9961 cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt gcaccggatt
    10021 tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat tcttttggtg
    10081 caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag ttagccatat
    10141 tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca aagttttctt
    10201 ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc aagccattcc
    10261 aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg aatccacgga
    10321 attcgattcc gtcaaacata cgtcctagta ctttagctgt atcttctgtg gattcttttt
    10381 tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga tcgatcgctg
    10441 cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc gcaatatttt
    10501 ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag tgttctgaaa
    10561 aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat aagcttcttc
    10621 cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt atttaagatt
    10681 aaagaggaaa tcccgacggc agctgacagt aagactgccg tcaggaaaca aattacttca
    10741 agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacgacct cgtgacaatt
    10801 cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg ttcgatacat
    10861 aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt gatccatcat
    10921 tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc agtacaagat
    10981 catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga
    11041 tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta ctttgaattg
    11101 ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca
    11161 ttgcacggtt gttcggaatt ttgattgcca acactttttc aaatcctgaa ttttttgcga
    11221 ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa atcccaacag
    11281 ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct ggattttcac
    11341 gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc aaaatatatt
    11401 ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact gtcatcccat
    11461 ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa
    11521 atggatagtc atcgtctgct gacagatcat atagactgct agaacgtact tcgatatctt
    11581 ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt gatagaagga
    11641 actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc aacattttat
    11701 tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt
    11761 aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa gttgtcatgt tcttcttgag
    11821 caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct ggtgtcaaat
    11881 tttccacttc ttgtccaggc cgtttcagta aaactgtctt taatttccct atttcagaga
    11941 aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac atgtatagga
    12001 tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaatgaga cattcctcac
    12061 attaatattc gataattaaa taattaaacg tttttctatt tttaagcgta atttattcat
    12121 tttttattct gtatacactt tcaaaaaaat atttattcat tgaaaaaatt tttttgaata
    12181 aattaaaaaa at
//
LOCUS       DAZBWR010000086        12144 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000086 DAZBWR010000000
VERSION     DAZBWR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.089"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACHGT5_002075"
     CDS             <1..566
                     /locus_tag="ACHGT5_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN7218046.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACHGT5_002076"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACHGT5_002076"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN7218047.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACHGT5_002077"
     CDS             1588..2160
                     /locus_tag="ACHGT5_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7218048.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACHGT5_002078"
     CDS             complement(2412..2669)
                     /locus_tag="ACHGT5_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218049.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACHGT5_002079"
     CDS             complement(2795..3610)
                     /locus_tag="ACHGT5_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218050.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACHGT5_002080"
     CDS             4127..5452
                     /locus_tag="ACHGT5_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN7218051.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACHGT5_002081"
     CDS             5445..5795
                     /locus_tag="ACHGT5_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218052.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="ACHGT5_002082"
     CDS             5792..5962
                     /locus_tag="ACHGT5_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218053.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHGT5_002083"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHGT5_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN7218054.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACHGT5_002084"
     CDS             complement(7113..8066)
                     /locus_tag="ACHGT5_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7218055.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACHGT5_002085"
     CDS             8678..10171
                     /locus_tag="ACHGT5_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN7218056.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACHGT5_002086"
     CDS             10306..10602
                     /locus_tag="ACHGT5_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN7218057.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACHGT5_002087"
     CDS             10703..11554
                     /locus_tag="ACHGT5_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218058.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACHGT5_002088"
     CDS             11575..11787
                     /locus_tag="ACHGT5_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218059.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4172 a         1863 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DAZBWR010000087        12125 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000087 DAZBWR010000000
VERSION     DAZBWR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.090"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..251)
                     /locus_tag="ACHGT5_002089"
     CDS             complement(87..251)
                     /locus_tag="ACHGT5_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218060.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            546..1874
                     /locus_tag="ACHGT5_002090"
     CDS             546..1874
                     /locus_tag="ACHGT5_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN7218061.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2076..2909
                     /locus_tag="ACHGT5_002091"
     CDS             2076..2909
                     /locus_tag="ACHGT5_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN7218062.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2925..3662
                     /locus_tag="ACHGT5_002092"
     CDS             2925..3662
                     /locus_tag="ACHGT5_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN7218063.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3817..4785)
                     /locus_tag="ACHGT5_002093"
     CDS             complement(3817..4785)
                     /locus_tag="ACHGT5_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN7218064.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5017..5850
                     /locus_tag="ACHGT5_002094"
     CDS             5017..5850
                     /locus_tag="ACHGT5_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN7218065.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5884..6723
                     /locus_tag="ACHGT5_002095"
     CDS             5884..6723
                     /locus_tag="ACHGT5_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7218066.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6749..7309
                     /locus_tag="ACHGT5_002096"
     CDS             6749..7309
                     /locus_tag="ACHGT5_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7218067.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7508..9229
                     /locus_tag="ACHGT5_002097"
     CDS             7508..9229
                     /locus_tag="ACHGT5_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN7218068.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9394..10536
                     /locus_tag="ACHGT5_002098"
     CDS             9394..10536
                     /locus_tag="ACHGT5_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN7218069.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10552..11763
                     /gene="thiI"
                     /locus_tag="ACHGT5_002099"
     CDS             10552..11763
                     /gene="thiI"
                     /locus_tag="ACHGT5_002099"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN7218070.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4130 a         2035 c         2520 g         3440 t
ORIGIN      
        1 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
       61 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      121 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      181 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      241 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      301 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      361 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      421 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      481 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      541 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      601 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      661 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      721 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      781 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      841 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      901 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
      961 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1021 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1081 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1141 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1201 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1261 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1321 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1381 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1441 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1501 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1561 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1621 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1681 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1741 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1801 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1861 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1921 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     1981 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2041 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2101 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2161 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2221 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2281 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2341 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2401 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2461 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2521 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2581 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2641 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2701 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2761 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2821 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2881 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     2941 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3001 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3061 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3121 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3181 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3241 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3301 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3361 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3421 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3481 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3541 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3601 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3661 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     3721 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     3781 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     3841 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     3901 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     3961 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4021 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4081 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4141 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4201 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4261 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4321 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4381 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4441 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4501 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     4561 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     4621 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     4681 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     4741 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     4801 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     4861 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     4921 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     4981 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5041 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5101 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5161 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5221 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5281 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5341 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5401 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5461 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     5521 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     5581 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     5641 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     5701 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     5761 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     5821 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     5881 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     5941 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6001 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6061 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6121 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6181 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6241 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6301 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6361 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6421 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6481 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     6541 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     6601 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     6661 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     6721 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     6781 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     6841 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     6901 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     6961 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7021 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7081 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7141 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7201 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7261 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7321 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7381 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7441 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7501 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     7561 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     7621 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     7681 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     7741 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     7801 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     7861 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     7921 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     7981 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8041 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8101 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8161 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8221 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8281 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8341 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8401 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8461 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     8521 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     8581 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     8641 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     8701 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     8761 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     8821 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     8881 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     8941 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9001 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9061 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9121 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9181 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9241 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9301 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9361 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9421 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9481 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     9541 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     9601 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     9661 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     9721 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     9781 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     9841 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     9901 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
     9961 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10021 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10081 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10141 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10201 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10261 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10321 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10381 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10441 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10501 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    10561 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    10621 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    10681 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    10741 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    10801 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    10861 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    10921 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    10981 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11041 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11101 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11161 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11221 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11281 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11341 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11401 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11461 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    11521 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    11581 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    11641 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    11701 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    11761 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    11821 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    11881 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    11941 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12001 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12061 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12121 ggttg
//
LOCUS       DAZBWR010000088        11867 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000088 DAZBWR010000000
VERSION     DAZBWR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.091"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..738)
                     /locus_tag="ACHGT5_002100"
     CDS             complement(253..738)
                     /locus_tag="ACHGT5_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN7218071.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    828..1173
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1218..1859
                     /locus_tag="ACHGT5_002101"
     CDS             1218..1859
                     /locus_tag="ACHGT5_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN7218072.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1878..2513
                     /locus_tag="ACHGT5_002102"
     CDS             1878..2513
                     /locus_tag="ACHGT5_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN7218073.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2531..3346
                     /locus_tag="ACHGT5_002103"
     CDS             2531..3346
                     /locus_tag="ACHGT5_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7218074.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3665..5101
                     /locus_tag="ACHGT5_002104"
     CDS             3665..5101
                     /locus_tag="ACHGT5_002104"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN7218075.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5290..7737
                     /locus_tag="ACHGT5_002105"
     CDS             5290..7737
                     /locus_tag="ACHGT5_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN7218076.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7967..9034
                     /gene="rlmN"
                     /locus_tag="ACHGT5_002106"
     CDS             7967..9034
                     /gene="rlmN"
                     /locus_tag="ACHGT5_002106"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN7218077.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9024..9230
                     /locus_tag="ACHGT5_002107"
     CDS             9024..9230
                     /locus_tag="ACHGT5_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218078.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9227..10330)
                     /locus_tag="ACHGT5_002108"
     CDS             complement(9227..10330)
                     /locus_tag="ACHGT5_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN7218079.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10882..11430
                     /gene="aac(6')"
                     /locus_tag="ACHGT5_002109"
     CDS             10882..11430
                     /gene="aac(6')"
                     /locus_tag="ACHGT5_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN7218080.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(11678..11797)
                     /locus_tag="ACHGT5_002110"
     CDS             complement(11678..11797)
                     /locus_tag="ACHGT5_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN7218081.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3944 a         1993 c         2541 g         3389 t
ORIGIN      
        1 gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa aaagaagcat
       61 ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag
      121 aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag
      181 aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa
      241 actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt
      301 ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac
      361 ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg
      421 catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc
      481 ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc
      541 gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa
      601 tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc
      661 aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg
      721 ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag
      781 agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga
      841 aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag
      901 ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg
      961 agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga
     1021 gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact
     1081 ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa
     1141 cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt
     1201 taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag
     1261 cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg
     1321 ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg
     1381 tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc
     1441 cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact
     1501 atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat
     1561 atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc
     1621 cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag
     1681 attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga
     1741 tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga
     1801 ctggtattct aacaatcgta atgaaacaag ttgaaggaaa attaaattat taccgctaac
     1861 tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa
     1921 aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc
     1981 cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca
     2041 gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac
     2101 aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa
     2161 acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag
     2221 cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc
     2281 tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg
     2341 tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc
     2401 gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag
     2461 tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga
     2521 gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt
     2581 tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa
     2641 aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg
     2701 gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac
     2761 ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat
     2821 cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac
     2881 aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa
     2941 ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt
     3001 atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta
     3061 tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat
     3121 ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat
     3181 aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac
     3241 attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa
     3301 aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg
     3361 tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct
     3421 tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag
     3481 tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt
     3541 cttactactc gaaactaagc gactttttca caacctcctt tacataattt tattttgttg
     3601 tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag
     3661 atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc
     3721 tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga
     3781 tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga
     3841 ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta
     3901 ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc
     3961 tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat
     4021 gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga
     4081 atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg
     4141 cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta
     4201 tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca
     4261 tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg
     4321 aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa
     4381 tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat
     4441 gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac
     4501 gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa
     4561 ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat
     4621 gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa
     4681 agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt
     4741 aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa
     4801 tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat
     4861 caattactta caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt
     4921 cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa
     4981 gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata
     5041 taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta
     5101 gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa
     5161 tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc
     5221 tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag
     5281 ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg
     5341 aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt
     5401 ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc
     5461 tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga
     5521 ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc
     5581 ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc
     5641 catactgggg aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa
     5701 caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt
     5761 acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga
     5821 ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt
     5881 ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg
     5941 gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca
     6001 aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa
     6061 agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt
     6121 tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta
     6181 ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat
     6241 gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta
     6301 ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa
     6361 attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag
     6421 gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg
     6481 gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa
     6541 gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat
     6601 ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat
     6661 aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt
     6721 tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt
     6781 aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg
     6841 atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg
     6901 gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa
     6961 gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt
     7021 gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg
     7081 atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt
     7141 attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt
     7201 ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt
     7261 gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc
     7321 ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa
     7381 ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa
     7441 gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca
     7501 gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg
     7561 gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta
     7621 gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa
     7681 gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac
     7741 ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca
     7801 aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt
     7861 ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat
     7921 catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc
     7981 catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa
     8041 attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga
     8101 aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc
     8161 tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact
     8221 ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg
     8281 tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa
     8341 aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta
     8401 ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga
     8461 gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg
     8521 attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa
     8581 ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa
     8641 tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga
     8701 agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt
     8761 gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa
     8821 gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata
     8881 ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt
     8941 aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg
     9001 aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta
     9061 ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt
     9121 ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg
     9181 tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct
     9241 tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt
     9301 taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt
     9361 tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg
     9421 gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca
     9481 tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt
     9541 tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt
     9601 taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg
     9661 catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac
     9721 gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc
     9781 cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata
     9841 agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt
     9901 ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag
     9961 tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt
    10021 caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg
    10081 ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc
    10141 cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt
    10201 ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga
    10261 cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac
    10321 tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga
    10381 tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa
    10441 taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa
    10501 aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt
    10561 gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt
    10621 atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca
    10681 aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt
    10741 ttctgtataa ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt
    10801 ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag
    10861 caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg
    10921 aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg
    10981 gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa
    11041 gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg
    11101 catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat
    11161 tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat
    11221 ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg
    11281 gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa
    11341 atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa
    11401 acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc
    11461 ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga aattccaact
    11521 tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta
    11581 gtttcgagta gtaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg
    11641 cttaataccg agaaactggc ttttgaacac cgtttattca gataagggac tgtgacaaga
    11701 cttttgtcac agtttctttt tgtttattct tttttatctt tgtatggcaa taatagacaa
    11761 tataacgaaa attatttaat ggcttttgga agaccatgga aaaccttgta caaagctatt
    11821 ttttctgaaa aattccttga aacctcacga aaaaatcatt attttat
//
LOCUS       DAZBWR010000089        11670 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000089 DAZBWR010000000
VERSION     DAZBWR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.092"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACHGT5_002111"
     CDS             133..636
                     /locus_tag="ACHGT5_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN7218082.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACHGT5_002112"
     CDS             763..1800
                     /locus_tag="ACHGT5_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFN7218083.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACHGT5_002113"
     CDS             1815..3512
                     /locus_tag="ACHGT5_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFN7218084.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACHGT5_002114"
     CDS             3505..3966
                     /locus_tag="ACHGT5_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFN7218085.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACHGT5_002115"
     CDS             3979..4839
                     /locus_tag="ACHGT5_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218086.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACHGT5_002116"
     CDS             4877..5266
                     /locus_tag="ACHGT5_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN7218087.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACHGT5_002117"
     CDS             5253..7700
                     /locus_tag="ACHGT5_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7218088.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACHGT5_002118"
     CDS             7705..9813
                     /locus_tag="ACHGT5_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN7218089.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACHGT5_002119"
     CDS             9810..10814
                     /locus_tag="ACHGT5_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN7218090.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..>11670
                     /locus_tag="ACHGT5_002120"
     CDS             10833..>11670
                     /locus_tag="ACHGT5_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN7218091.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVS"
BASE COUNT         3894 a         1762 c         2493 g         3521 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa
//
LOCUS       DAZBWR010000090        11662 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000090 DAZBWR010000000
VERSION     DAZBWR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.093"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            326..532
                     /locus_tag="ACHGT5_002121"
     CDS             326..532
                     /locus_tag="ACHGT5_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN7218092.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(734..1735)
                     /locus_tag="ACHGT5_002122"
     CDS             complement(734..1735)
                     /locus_tag="ACHGT5_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN7218093.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1740..3653)
                     /locus_tag="ACHGT5_002123"
     CDS             complement(1740..3653)
                     /locus_tag="ACHGT5_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN7218094.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3821..4327)
                     /locus_tag="ACHGT5_002124"
     CDS             complement(3821..4327)
                     /locus_tag="ACHGT5_002124"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN7218095.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4487..4927
                     /locus_tag="ACHGT5_002125"
     CDS             4487..4927
                     /locus_tag="ACHGT5_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN7218096.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4953..6068)
                     /locus_tag="ACHGT5_002126"
     CDS             complement(4953..6068)
                     /locus_tag="ACHGT5_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218097.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6113..6469)
                     /locus_tag="ACHGT5_002127"
     CDS             complement(6113..6469)
                     /locus_tag="ACHGT5_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218098.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6767..7741)
                     /gene="sgrA"
                     /locus_tag="ACHGT5_002128"
     CDS             complement(6767..7741)
                     /gene="sgrA"
                     /locus_tag="ACHGT5_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN7218099.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7937..8776)
                     /locus_tag="ACHGT5_002129"
     CDS             complement(7937..8776)
                     /locus_tag="ACHGT5_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN7218100.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8961..9848
                     /locus_tag="ACHGT5_002130"
     CDS             8961..9848
                     /locus_tag="ACHGT5_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN7218101.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10442..11137)
                     /locus_tag="ACHGT5_002131"
     CDS             complement(10442..11137)
                     /locus_tag="ACHGT5_002131"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFN7218102.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11121..11519)
                     /locus_tag="ACHGT5_002132"
     CDS             complement(11121..11519)
                     /locus_tag="ACHGT5_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN7218103.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3478 a         2313 c         1747 g         4124 t
ORIGIN      
        1 taggttatca tttgtaattt tatttcacag ttgatatgat tgttatgtaa aataaaaaag
       61 tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat cttttctatt
      121 atattcccat attttctatt attggtctag acgtatgatg gtaaactttg tacctatctg
      181 attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg ctagatgatc
      241 attttattca catcctaaca aacttgtcac atttatagat tcttaatcta tttatcatac
      301 aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc tttttttcgc
      361 tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca ataacaacgg
      421 aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc ttcttgcctc
      481 cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct gacttataaa
      541 atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc gaataaaagg
      601 tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa ccatgaaaag
      661 cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac aacctctttt
      721 gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt
      781 gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc tccgcataat
      841 ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct tccatttgtt
      901 tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat
      961 gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta ttatcaaagt
     1021 aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact tgagtaagtg
     1081 cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg ttctccaaag
     1141 tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac aaatattctc
     1201 cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa tctgcttgat
     1261 cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact ggtgtaggat
     1321 gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc gtgtctgaac
     1381 atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat gcgcctccat
     1441 gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag atatccatat
     1501 tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt tgttcgattt
     1561 tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg tcaaattgtt
     1621 ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat acagtgacta
     1681 aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat ttcattcatt
     1741 taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa aaatccacct
     1801 gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct tttttttgca
     1861 tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc acttgattga
     1921 tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc ttgctttttt
     1981 ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt ctcattaaaa
     2041 atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt cttttgctct
     2101 gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc tttgagtaga
     2161 cttttattgt tttcatccac atactttacg tttatctgat catggaacac tactttcaaa
     2221 aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt tccatgccct
     2281 gctccttcta ctaataaaag atctttatac cctcctgata actcatataa tttttgcgcg
     2341 ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg caatgaatct
     2401 gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg atcaaaatag
     2461 tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat gatgtctaac
     2521 tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt
     2581 tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt gacaggaata
     2641 tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata aagtaaaatc
     2701 ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc atctgactca
     2761 tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga atcaacaaat
     2821 aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat cccgttattc
     2881 caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg atcgacataa
     2941 tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg atgaagttgt
     3001 tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc tgtacctcca
     3061 agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag ataagtagca
     3121 atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag ctcagtgact
     3181 aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat ttctgtcatc
     3241 tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt accgatatca
     3301 tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc gtcgtttgac
     3361 atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt cgtttcttga
     3421 tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta
     3481 ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc ccgagattcc
     3541 gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt acttacagca
     3601 aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt cactcctttt
     3661 tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa ataaatggtt
     3721 tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc agaatggata
     3781 aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc aaaaatgtca
     3841 gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag
     3901 gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct aatccatgag
     3961 caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat tcgatcgaat
     4021 gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa ttcgcatggg
     4081 tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc tcccatggtt
     4141 ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt ttcccaatcg
     4201 tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat tcgtgtatcg
     4261 gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg tcggctgtgt
     4321 ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt atctagctag
     4381 ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact tttcgactat
     4441 ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga tgattaaatt
     4501 gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga
     4561 tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg aatttatcca
     4621 cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt ctattagtta
     4681 taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata ttgagattcg
     4741 tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa aacttttgac
     4801 tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa agcaagagct
     4861 caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc tttttgcaat
     4921 gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa tttttccaat
     4981 atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg cgcccaatca
     5041 ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc atatgccttt
     5101 ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga tgcactatat
     5161 tttattgggc gttcagtcat agataatatc cttttctttg cccataattc ctgtgtttta
     5221 ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa aatatccctg
     5281 actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt attaagctct
     5341 ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat cactggaaat
     5401 atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt cttttttgtc
     5461 ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata atttttttct
     5521 gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga cgattcaatc
     5581 cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc caaataggtg
     5641 ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat gtgattcatc
     5701 gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt
     5761 atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga gtggccttgt
     5821 ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc tatactacgc
     5881 gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt tatacttgat
     5941 aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg
     6001 atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga taaatgatag
     6061 tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa acttatataa
     6121 ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa
     6181 tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa aactgagaag
     6241 aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat tctggatacc
     6301 tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg gtgtagttta
     6361 gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa caatagttca
     6421 aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa gtacatactc
     6481 ctttttatct attatttaga cttattccga cagctacatg aataacgtac aaatagattc
     6541 gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta ttcttgctct
     6601 tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta aacaaaaata
     6661 gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa atattcactc
     6721 aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat ttcactcgat
     6781 ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca cccatccatg
     6841 taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt ctattatctg
     6901 tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc tgttttagca
     6961 cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg ataggtacca
     7021 aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac
     7081 tctcactact catgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     7141 tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg
     7201 tgttttctgt gctactttca ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt
     7261 ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca actgtccaac
     7321 tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa
     7381 ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt attgtatact
     7441 caataccagc ggtccattgt acgtctttta aataaacttc cccaataact ttttctgaac
     7501 ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca ttcacgcttt
     7561 tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca atccgatcag
     7621 ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta ctgattccaa
     7681 tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct gttttcttca
     7741 tttctatcac tctccatata attaccttct atttgataaa ggaattatat aaaatgaaaa
     7801 tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga
     7861 atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc gatatcagcc
     7921 ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc tttgttttat
     7981 atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt gatatagatg
     8041 cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc agagtggaga
     8101 aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct tgtgtccaat
     8161 tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag ataagtgcgc
     8221 tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg ttaggattca
     8281 atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac ggaccaaatc
     8341 taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc cccactgcaa
     8401 taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa tctaaccgat
     8461 acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt ccaacaacga
     8521 gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa tcttctattc
     8581 tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct aaatgtccga
     8641 ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc gcaagtgcat
     8701 tattgacaag aattcctaaa acaaaaccaa ctaagactat taaaacagat ccaatcaacc
     8761 gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc cttttctttc
     8821 ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt tttacgaact
     8881 aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc ttctgttaga
     8941 ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta attatgtgcc
     9001 tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa acgattgcaa
     9061 cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa gttaaatcaa
     9121 ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat gtatacggaa
     9181 aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa aaactcggtc
     9241 caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat aagaaactct
     9301 ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta atagataaag
     9361 atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc attctatttt
     9421 cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca
     9481 agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga aaaatgaatt
     9541 ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt aaactaaaaa
     9601 acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag caaagctatt
     9661 atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa tacaaatctg
     9721 agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa
     9781 tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta aaaaatatat
     9841 tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt gatttttgga
     9901 aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg gccttttttt
     9961 atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat ttaaaaaagt
    10021 tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat gcttccctat
    10081 tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc gactgaaaag
    10141 ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt ctgttatact
    10201 agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg aaatttattc
    10261 aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat acatcgttta
    10321 aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat gaatattgaa
    10381 tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg gagaaattga
    10441 ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg aaagtttgca
    10501 cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct aatacttgat
    10561 ctagtagctc tttacctaac gttatcgcat gagctccaga tcgaatggct gtagtgactt
    10621 gttcacaatt tttgaaacta gctgctaata tttttgttgg tctttgcgta cgttcgatct
    10681 cagtccgcaa cgaatcaatc actttcatag catcaatgcc ttgattttgc atcctgttat
    10741 aataaggtgc aatataatct gctccattat taatagctaa atacccttgt aattctgtgt
    10801 aaatagctgt accggtaatc aaataacctt cttttttcaa ctttttgatc gcttgaaggc
    10861 ctgcttgatt caccggcacc ttgataaacg tatcttttcc cagttcttct atgataactc
    10921 gagcatcctc tatcatttct tcttccgttc ttcctattgt ctgaatatgg ataggaatct
    10981 gtccgattat ttttttgatt tctttcatat gcgagaagaa gtttgacgct ccttcttttt
    11041 tcagaattgt aggatttgtt gttacgcctg ttaaattcaa aaactgttga caagaataaa
    11101 tactttttgt atttgctgta tcaagcatga tttccatata gctttccccc attctgatcc
    11161 tcttgatcga ttttaatgat tgtccccttt cttaaatgcg gcatttcctt atcttctaca
    11221 tacattgttc ctggaagttc cggctcatga gatccatcaa attttatcgt gatatgtcct
    11281 aattcattca aattagtttg gacaacatct cctactgctg tgattacata catttgttta
    11341 tcaaaacaga gcttcatatc acaagttatt tcttttctta cctgattcaa ttcaatctta
    11401 taacaataat ctgttaatgt ttccggtgct tcatctccaa ataaaatcat tattttttct
    11461 ttcctgaata cttctgcttc tataccaaca tctgttacaa ccgtttcaaa gatcgtcatc
    11521 tctgttcctc ctcgtaaaat atcatttgtc taatagtttt tattcaaaat gtttctagat
    11581 tataaaattt attgaataac acattcctaa cggaatttgt ttcgatattg tgtgatttct
    11641 cagagtacaa tggatgtatg ga
//
LOCUS       DAZBWR010000091        11571 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000091 DAZBWR010000000
VERSION     DAZBWR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.094"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHGT5_002133"
     CDS             6..305
                     /locus_tag="ACHGT5_002133"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7218104.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHGT5_002134"
     CDS             378..1739
                     /locus_tag="ACHGT5_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN7218105.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHGT5_002135"
     CDS             1761..2021
                     /locus_tag="ACHGT5_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218106.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHGT5_002136"
     CDS             2035..2787
                     /locus_tag="ACHGT5_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN7218107.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHGT5_002137"
     CDS             complement(2881..3486)
                     /locus_tag="ACHGT5_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN7218108.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHGT5_002138"
     CDS             complement(3539..4513)
                     /locus_tag="ACHGT5_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN7218109.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHGT5_002139"
     CDS             complement(4891..5610)
                     /locus_tag="ACHGT5_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7218110.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGT5_002140"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGT5_002140"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN7218111.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHGT5_002141"
     CDS             7161..8558
                     /locus_tag="ACHGT5_002141"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN7218112.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHGT5_002142"
     CDS             8639..9079
                     /locus_tag="ACHGT5_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN7218113.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHGT5_002143"
     CDS             9227..9553
                     /locus_tag="ACHGT5_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN7218114.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHGT5_002144"
     CDS             9554..10303
                     /locus_tag="ACHGT5_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN7218115.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHGT5_002145"
     CDS             complement(10383..10838)
                     /locus_tag="ACHGT5_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN7218116.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHGT5_002146"
     CDS             complement(11265..11540)
                     /locus_tag="ACHGT5_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218117.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3650 a         2009 c         2351 g         3561 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat g
//
LOCUS       DAZBWR010000092        11432 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000092 DAZBWR010000000
VERSION     DAZBWR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.095"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..290)
                     /locus_tag="ACHGT5_002147"
     CDS             complement(66..290)
                     /locus_tag="ACHGT5_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFN7218118.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(488..2068)
                     /locus_tag="ACHGT5_002148"
     CDS             complement(488..2068)
                     /locus_tag="ACHGT5_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN7218119.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2469..3845)
                     /locus_tag="ACHGT5_002149"
     CDS             complement(2469..3845)
                     /locus_tag="ACHGT5_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN7218120.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3973..5061)
                     /locus_tag="ACHGT5_002150"
     CDS             complement(3973..5061)
                     /locus_tag="ACHGT5_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN7218121.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5233..5667
                     /locus_tag="ACHGT5_002151"
     CDS             5233..5667
                     /locus_tag="ACHGT5_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7218122.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5745..6278)
                     /locus_tag="ACHGT5_002152"
     CDS             complement(5745..6278)
                     /locus_tag="ACHGT5_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN7218123.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACHGT5_002153"
     CDS             complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACHGT5_002153"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN7218124.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACHGT5_002154"
     CDS             complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACHGT5_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN7218125.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8447..9829
                     /gene="rlmD"
                     /locus_tag="ACHGT5_002155"
     CDS             8447..9829
                     /gene="rlmD"
                     /locus_tag="ACHGT5_002155"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN7218126.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10003..10581)
                     /locus_tag="ACHGT5_002156"
     CDS             complement(10003..10581)
                     /locus_tag="ACHGT5_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN7218127.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10903..11373
                     /locus_tag="ACHGT5_002157"
     CDS             10903..11373
                     /locus_tag="ACHGT5_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN7218128.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3364 a         2293 c         1949 g         3826 t
ORIGIN      
        1 aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt
       61 tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt
      121 ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt
      181 gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc
      241 tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
      301 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
      361 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
      421 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
      481 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
      541 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
      601 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
      661 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
      721 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
      781 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
      841 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
      901 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
      961 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
     1021 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
     1081 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
     1141 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
     1201 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
     1261 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
     1321 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
     1381 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
     1441 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
     1501 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
     1561 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
     1621 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
     1681 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
     1741 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
     1801 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
     1861 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
     1921 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
     1981 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
     2041 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
     2101 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
     2161 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
     2221 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
     2281 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
     2341 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
     2401 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
     2461 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
     2521 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
     2581 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
     2641 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
     2701 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
     2761 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
     2821 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
     2881 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
     2941 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
     3001 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
     3061 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
     3121 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
     3181 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
     3241 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
     3301 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
     3361 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
     3421 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
     3481 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
     3541 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
     3601 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
     3661 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
     3721 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
     3781 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
     3841 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
     3901 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
     3961 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
     4021 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
     4081 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
     4141 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
     4201 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
     4261 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
     4321 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
     4381 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
     4441 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
     4501 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
     4561 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
     4621 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
     4681 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
     4741 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
     4801 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
     4861 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
     4921 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
     4981 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
     5041 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
     5101 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
     5161 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
     5221 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
     5281 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
     5341 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
     5401 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
     5461 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
     5521 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
     5581 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
     5641 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
     5701 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
     5761 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
     5821 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
     5881 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
     5941 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
     6001 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
     6061 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
     6121 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
     6181 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
     6241 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
     6301 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
     6361 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
     6421 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
     6481 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
     6541 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
     6601 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
     6661 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
     6721 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
     6781 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
     6841 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
     6901 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
     6961 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
     7021 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
     7081 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
     7141 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
     7201 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
     7261 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
     7321 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
     7381 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
     7441 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct
     7501 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
     7561 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
     7621 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
     7681 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
     7741 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
     7801 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
     7861 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
     7921 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
     7981 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
     8041 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
     8101 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
     8161 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
     8221 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
     8281 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
     8341 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
     8401 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
     8461 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
     8521 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
     8581 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
     8641 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
     8701 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
     8761 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
     8821 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
     8881 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
     8941 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
     9001 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
     9061 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
     9121 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
     9181 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
     9241 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
     9301 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
     9361 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
     9421 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
     9481 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     9541 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     9601 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     9661 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     9721 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     9781 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     9841 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     9901 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     9961 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    10021 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    10081 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    10141 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    10201 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    10261 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    10321 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    10381 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    10441 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    10501 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    10561 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    10621 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    10681 aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga
    10741 gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt
    10801 taataacaac aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg
    10861 agccttcatg gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat
    10921 attcgttttt gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt
    10981 agcggaaatc ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc
    11041 tggatatttg ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg
    11101 ttacttaatc acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag
    11161 ttcattttta attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca
    11221 gcaatcgata ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg
    11281 catcatcgcc tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact
    11341 ctattgaatt attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt
    11401 aacacataga aaaagtgcca cctaagttag at
//
LOCUS       DAZBWR010000093        11253 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000093 DAZBWR010000000
VERSION     DAZBWR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.096"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHGT5_002158"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHGT5_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN7218129.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHGT5_002159"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHGT5_002159"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN7218130.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHGT5_002160"
     CDS             complement(2169..2906)
                     /locus_tag="ACHGT5_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN7218131.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACHGT5_002161"
     CDS             complement(2997..3524)
                     /locus_tag="ACHGT5_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218132.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHGT5_002162"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHGT5_002162"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN7218133.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHGT5_002163"
     CDS             complement(4401..4886)
                     /locus_tag="ACHGT5_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN7218134.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACHGT5_002164"
     CDS             complement(5039..5236)
                     /locus_tag="ACHGT5_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218135.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHGT5_002165"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHGT5_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN7218136.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHGT5_002166"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHGT5_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN7218137.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACHGT5_002167"
     CDS             9211..9423
                     /locus_tag="ACHGT5_002167"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN7218138.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACHGT5_002168"
     CDS             9425..11104
                     /locus_tag="ACHGT5_002168"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN7218139.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3588 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaa
//
LOCUS       DAZBWR010000094        11227 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000094 DAZBWR010000000
VERSION     DAZBWR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.097"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..1448
                     /gene="lysA"
                     /locus_tag="ACHGT5_002169"
     CDS             189..1448
                     /gene="lysA"
                     /locus_tag="ACHGT5_002169"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN7218140.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1435..2235
                     /gene="dapF"
                     /locus_tag="ACHGT5_002170"
     CDS             1435..2235
                     /gene="dapF"
                     /locus_tag="ACHGT5_002170"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN7218141.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2238..3119
                     /gene="dapA"
                     /locus_tag="ACHGT5_002171"
     CDS             2238..3119
                     /gene="dapA"
                     /locus_tag="ACHGT5_002171"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN7218142.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3116..3832
                     /gene="dapB"
                     /locus_tag="ACHGT5_002172"
     CDS             3116..3832
                     /gene="dapB"
                     /locus_tag="ACHGT5_002172"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN7218143.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3838..4533
                     /gene="dapD"
                     /locus_tag="ACHGT5_002173"
     CDS             3838..4533
                     /gene="dapD"
                     /locus_tag="ACHGT5_002173"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN7218144.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4549..5550
                     /locus_tag="ACHGT5_002174"
     CDS             4549..5550
                     /locus_tag="ACHGT5_002174"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN7218145.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5547..6908
                     /locus_tag="ACHGT5_002175"
     CDS             5547..6908
                     /locus_tag="ACHGT5_002175"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN7218146.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6868..8019
                     /locus_tag="ACHGT5_002176"
     CDS             6868..8019
                     /locus_tag="ACHGT5_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN7218147.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7994..9163
                     /locus_tag="ACHGT5_002177"
     CDS             7994..9163
                     /locus_tag="ACHGT5_002177"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN7218148.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9338..10717
                     /locus_tag="ACHGT5_002178"
     CDS             9338..10717
                     /locus_tag="ACHGT5_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN7218149.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10998..11227
                     /locus_tag="ACHGT5_002179"
                     /pseudo
     CDS             10998..11227
                     /locus_tag="ACHGT5_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3739 a         1955 c         2503 g         3030 t
ORIGIN      
        1 ataaatagct tctaatagtt cgggtatttt gtttttataa ccatttttat tcttattttt
       61 aaaaaaatat ttcgaaatat actagacaat tcagaaaatt ttgtttaata ttattccaat
      121 aaaattcaaa tctttatcaa atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga
      181 atgaaaaaat gacagaattg ataattggcg gagtcacagc aagcacattg gcagaaaagt
      241 ataagacacc tctgtatgtg tatgatgaag aaaagatgag acaaacgatg ctgtcattca
      301 agcgaggctt tgtatcgaaa cgttttgata caaaagtgct ttacgcctct aaagcattcc
      361 aaacgattga gatgctgcat ttagttcgtg aatatggatt cgggctagat gttgtcagtg
      421 gtggggaaat ctataccgct ttgcaagctg attttcctag agatactatc tacttccatg
      481 gtaacaacaa gacgccagag gaactgatct atgcacttga aaatgatttg ttgcatattg
      541 ttgccgataa tttgatggaa gttgaattat tagcagaatt aagccaacgc tatttaaaaa
      601 aaatctgtat catgcttcgg ttgaatgtcg gagtagaagc acatactcat gaatatatcg
      661 tcactgcgca tattgattca aaattcggca tgagctatga aagtaaagag tgccaacaag
      721 cccttcagtt gataaaagaa agcagctatc tagagttaga aggatttcat gctcatatcg
      781 ggtcacaaat ttttgatgtt actgcttggt tagctgaaat cgacaaattg acgaattact
      841 taaccgattt tgaccaacct ctttccttga atcttggcgg agggtttggg attcgttata
      901 cacaaaatga tcagccgatc ccagtcgaag aggcactaaa aaaactgatt gaatatacgg
      961 aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa
     1021 gtattgttgg agaagctggt acaacactat acacgattgg tttcatcaaa aagacaccac
     1081 ataaaaaata ttactttgta gatggcggga tgacagataa tatccgtcca gctctgtatc
     1141 aggcagaata cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga
     1201 cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct tatttgccga
     1261 aagcagaatc gggtgatctt ttagctgttt attccacggg agcttatgga tactcgatga
     1321 gcagcaacta caatcgagcg acacgaccag cagttgtttt tgtcaataat ggccaatcaa
     1381 gactgattgt tcgcagacaa gcattttctg atctactaag gggggaagtt tcctatgatc
     1441 cttcataaat ataatggctg cggaaatgat tttatcttat tagattacgc agaagacaca
     1501 gattatagtc aattagccgc ttctttatgc acaaaagaaa aatatgatat agacggattg
     1561 atcgccgtaa aaatcgatcc gttggaaatg atctattaca acaaagatgg cagccgcgcg
     1621 ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg taaatgatca gaacatgatg
     1681 aatgaacagg aattcaacgt agagacatta gcaggcacaa gaaaaattac ggtgttggat
     1741 ccctcacctt tttattgcag cgtagatatg ggagtgccag atgattctcc ggaaaaaatc
     1801 aaagctaatc aaacttatcc aataaaaaac caggaaatag agatcaatgg tgaaaaagta
     1861 acgatcacta gtttattttt aggaacgatt cataccgttg tttttgttga aaatgcgcgt
     1921 gaggaagtct ggagaaaaag aggtgaactg atctgtcatc atcctttgtt caaagaaaaa
     1981 accaatgtca actttgtaga agtagtcaat gaacaagaat tgatcgtccg tacctatgaa
     2041 agaggagtag gttggacact tgcttgcggt acaggttgct gtgcctctta tgtggttgct
     2101 aaggaaactg ggaaaatttc agcagatcag gtagttgttc acttggaaca aggagacttg
     2161 atgatctctg gtgaggatgc gatacaaatg gctggcccag cagttcatga atggacaaga
     2221 atattggagg aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg
     2281 aagagggatc agtagattat gctggtttgg aaaaattgat ccaattccat ttagatgaac
     2341 aaacagatgg attattagtt ttaggtacaa caggagaatc ttctacgctc acacaatctg
     2401 aggaggagca gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg
     2461 caggagctgg aacgaataat acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt
     2521 tgggggcaga cgcacttttg gtcatcaccc cttactacaa caaaacaagt gatgcaggac
     2581 tagctgctca ttttacagca attgcagaag cctcaccgat tccgcttatt ttgtataatg
     2641 ttccttctag aacaggcatg tcgattccaa tccatgtatt agtaaattta gcagaacatc
     2701 ctaatatcat tggtttgaaa gaagcctcag gagatatggc ttatgtgatg gatgccgctc
     2761 ggttgattgg agaagaattc ttcctttaca gtggaaatga tgatttgatc ctaccagtaa
     2821 tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa tatccaacct aaaatagtcc
     2881 atgaattagt aaaagatacg caggacggaa gatggcagca agcaaaagaa aagcaattaa
     2941 atgcacttga actgatccat gccttgttca gtgagaccaa tcctattcct gtcaaagcag
     3001 ccatgtcatt attagactta ccttccggac ctctacgcct gcctttagtt tctctttcaa
     3061 aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa
     3121 aatcggactg attggcagtg gacagatggg aaatagagtc gaagaagttg cacaagaaag
     3181 aggagatacg gtccttcttt tacggacggc acctaaagaa gcaactacgc ttgcttttac
     3241 ggaactgcca gaactgctta ttgatttttc tcatccagat aatttagaga aaatcctttc
     3301 gtatagctta tcatatcaac ttccgttagt cattggcacg acaggttata cacaatcaca
     3361 gttccaagaa ataaaagaac atgccaaaca tgtacctgtg atgtatagtg ctaatttttc
     3421 gttcggcatc atggtgatga atcaactaat caagcaagca gctgcaatgt tgcaaggctg
     3481 gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc
     3541 aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat acaactggca
     3601 agggaaaaaa agagaagaaa accaaattgg tgtacacagt attcgtgcag gttctttacc
     3661 tggtgaacat gatgtattgt ttgctgctac tgatgaaata tttacgatca aacatgaatc
     3721 tttttctaat cgaatctttg ctgcaggcgc agtagaagca gcggactggt tgaaagatca
     3781 gtcaccaggt tattacaaat tagaagattt attaatggac aaaggggtgt aaaaaacgtg
     3841 gaagctcaag aaatcatccg ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc
     3901 tatctgaatg taaccgagcc aatcgaattt aaagaaagta aagtttttgg tgaaggaagc
     3961 agcttcgttg tttttggtga ctatgagacg attgctccgg ttttagaagc tgaatcagaa
     4021 aaaatcattg aaatcgaaat cgaaactgct gcaagataca gcgcagttcc tttgctagat
     4081 attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta tcagagatca agtgtcaatc
     4141 ggcaacaatg ccgtgatcat gatgggagcg attatcaata ttggtgcagt aatcggtgaa
     4201 aacacgatga ttgatatggg tgctgtctta ggtggacgag cgacagtggg caaaaactgc
     4261 catattggtg caggagctgt cttagcaggt gtgatcgaac cagcatcggc caaaccggtt
     4321 atcgtagaag acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc
     4381 ggaaaagatg cagtcgtggc agcaggagca gtggttttag aagatgtggc agcagaaact
     4441 gttgtcggtg gaatacctgc acgtgtatta aaaataagtg atgatcagac aaaagaaaat
     4501 acagcattga ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat gcgcgaaata
     4561 gatacctgta ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa agtgttagaa
     4621 gaatatgatt ttccagtagg acgattgaaa ttattagctt caaaacgttc agccggtaaa
     4681 gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat tgacagaaac ttcatttgaa
     4741 ggatatgatt tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc
     4801 gcacgaagtc agggactatt tgtcatagat aattcctcag catggcggga agattcggat
     4861 attccattaa tcgttccaga ggtcaatctg gaagactata cattgaatcg gctgattgca
     4921 aatccaaatt gttcaaccat tcaagcggta cttccgctta aagctttaca agaagccttt
     4981 ggtatccagc gagtcaatta ttcgacctat caagcagttt ctggttccgg gcaaaaaggc
     5041 attgaagatc ttcatcgtac gcaaaatgac gaggaaccag ttttgtatcc ttataatatt
     5101 agccaaacgg tgattccgga aatcgatcag cctttggaaa atgggtatac aaaagaagaa
     5161 cagaaaatga taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca
     5221 acctgcgtac gtgtgccaat cgaaaatgga catggagtct ctgtggcagt tcaactgcaa
     5281 aaagagttta cgacggaaga aattagagag tgtctcagcc agtttccagg gattcaatta
     5341 gaagatgatc tagcagcaca taaatatccg acgagcattc ttgctagagg aacagatatg
     5401 gtttatgttg ggaggatcag aaaagacact tctttagaga acggattgtt gttctatacg
     5461 actgccgata acatacgcaa aggagccgct gcgaatgccg tacagattgc tgcagcttta
     5521 atgcagaaaa tagcggaggt ggcaagatga gtaatacgct gaaagtagcg aaattcggcg
     5581 gaacatcgat gtctcatgaa gagcaattca aaaaagtccg tacaattgtt ctttcagatc
     5641 ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg tacgtcagat gatgacaaag
     5701 taacagactt actttatttg atttatgctt accttcagca tcatgttgat tggacacccc
     5761 tttggaaaag gatttgtgat cgtttcattc aagtaagaga tgttcttgcc ctgcagttac
     5821 ctattgaaga tgaactagca cgcatacaga aacaactgga agacgaaaag ataactgaag
     5881 attatttagt aagtagaggt gaatatctga ctgctcaact gatggctgag tatcttggtt
     5941 accagtttat tgacgcaaaa gacctgattt tctttgacta tgatgggaaa atcgatcagg
     6001 aaaaaacagc acaggaaatc acaaaagcat ttaaaagata tccgaaaatg gttgttccag
     6061 ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca
     6121 ttaccggcgc gatactggcg aagatgttgc aagcggaaag ttatgagaat tggacggatg
     6181 tttcaggaat catgatggct gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa
     6241 tctcttttag agaactgaga gagatggctt atatgggggc taacgttctt catgaagcag
     6301 ctgttttccc agttcaagag gctgatatcc ccatccaaat aaaaaatacc aatgatcctt
     6361 tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa
     6421 tagctggacg caaggatttt ctttccatca ctttattcaa acgtcatatg tccgatgaaa
     6481 tcgggtttat ctggaaagca atgagcattt ttgctaaaca tgagatatca atcgaacata
     6541 ttccttcagg gatcgacaat atcggagtga tcgtctcagc agaagcgatt gccgataagt
     6601 tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg
     6661 aagacttagc tttgatctct gtcgttgggg gtccgcataa agaattgatt ggattatctg
     6721 ggaaagtgtt atcgatattg aacaaattag aaattcgcac atcgatctta tcacaaggag
     6781 cacaggagtt gaatctgatc atcggtgtac cgaataatca atatgaaaca gtagtgaagg
     6841 gtatctatga agggatggtg aatacggatg ctgaaagaat atcgaatggc actccatcaa
     6901 attcctgaaa tcgggtttca tgaattcaag acaaaaaaat atttatatga acaagtaaaa
     6961 aacagcgagg ccgttatcca tgaaatcggc gaaacaggac tactccttta ttttgataat
     7021 cagcaagaag aaacgattgc ttttcgaaca gatatcgatg cactgccagt caaagaagaa
     7081 acgaatctct cttttgcttc taagcatgaa ggttatatgc atgcatgtgg tcatgacgga
     7141 catatggcga tgcttttagg gttgactgac tttataacta aacacattga ggatcttatt
     7201 tataatgtag tactgatcta tcagccatca gaagaaattt ccggtggcgc acaaagcgtt
     7261 attcagtcag gattgttgga taaatatgaa gtacaagcaa tatttggctt tcatctatgg
     7321 cctggactag ctaagggaga agttttctct cgaccaggac ctttgatggc acaaagcagt
     7381 gaaacagaca ttatcattaa aggccagtct gcacatattg cttctagtga aaaaggaatc
     7441 gatagtctgg aggcagctgt acgatttttg aagaatgttt atgattttga ccggcaatta
     7501 ccggcagagt ggaatcatct aatgaaattt ggccaaatcc aaggtggaac gatccgaaat
     7561 gttttagctg gagaagttat tgtatccgga agtatccgtt cttacagttc agatatgcaa
     7621 aattatttga agaggggatt agaccattta gcagaacagt ttcactctac agctactatt
     7681 tcttttcgct ataatgacgg ctatccagct gtaatcaacc atagaggctt atatgcagca
     7741 ctgttagatt caaacaaact acatgaatta gatgctccag ttcttcaagc cgaagatttt
     7801 ggcatatata cgcaacaata tccatgtgta tttttctttt taggtgtcgg tgatacccct
     7861 gctttacatg atccgacgtt cgattttgat atggatgttt tagaaaatgg tgtagaatgg
     7921 tacaaaacga ttctttttac agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag
     7981 gagcgagtaa ataatgatga atttatccaa tcgatttagt cctaggcttt ctagaataga
     8041 agtatcaaaa atacgacaat tcgatcagca aatatcgtcg ataccagatg tgatcaaact
     8101 caccctggga gaaccggatt ttccaactcc tgaacatgtc aagcaagcag gaatagcagc
     8161 gattgaagaa gacttttctc actataccgg aatgagagga ttagaggaac tgcgggaagc
     8221 ggcttgtata tttcagcaac agcgttatgg attaacttat gatccgcaaa cggaagtttt
     8281 gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt tggaagaagg
     8341 agacaaggtt cttattccag cgcctgctta ttcaggatat cagccattag tggaactagc
     8401 tggtgctgaa ttgatcccga tagatacttc tgacacaggg tttgtctgtc agccggaaca
     8461 atttgaacga gcatttgaac aatatggatc agctgtcaaa gcagtcattt taaactatcc
     8521 taacaatcct actgggacga cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt
     8581 aaaaaaatat ccggtatttg ttataagtga tgaagtatat gctgaattga cttatagtgg
     8641 aacgcatatg tccatagcta cttatcttcc agaacaaaca atcgtgataa gcgggttatc
     8701 caagtcacat gcgatgactg gttggcgagt gggctttatt tttgctcaaa aagcccttat
     8761 tgacgaacta attaaagtcc atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa
     8821 agctgcggct gaagcgctaa taaatggggt gaaagatagc gagaatatga aagagcaata
     8881 tcaaacacga agagactatc tggtccagca attggcgcct ttaggatttg aagtgaccca
     8941 gccaaacggt gcattttact tgttttgcaa actcccagct gccattcaaa ctgactcttg
     9001 gcagttttgt gtagaattag cggaacaggc aaaagttgcg tgtatcccag gaattgcatt
     9061 tggcccagaa ggagaaggtt atatccggat ttcttatgct agcagcatgg aaaaattaca
     9121 tgaggcctgc aaacggatca aagacttttt aacagaacag tgattcatag attttatcaa
     9181 aaaaacaatc gttaattttg cggtatattc tgcaaggaga cgattgtttt ttaaatttag
     9241 ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa tgttaagttt aaattgttta
     9301 gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat
     9361 tgtaggtgca tctcatggag ggcatcaatc gatcttggaa ctattgacac gatacggtga
     9421 aaatgtagat atcacgctat ttgaagcagg agactatatt tccttcatgt catgtggtat
     9481 ggagctgtat ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga
     9541 aaactttccg caatccaatg ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc
     9601 cgataaaaaa acagtaacgg ttactagcgc atcagataac gaaacaaaag aatatcctta
     9661 tgacaaattg attttgagtt ctggtgtcaa accaaacaat ctgccagtac caggagcaga
     9721 tctagaaaat gtctacttga tgcgtggata caattgggca accaaaatca aagaaaaatt
     9781 aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc
     9841 agcagaagca agccgaaaag ctgggaaaga agtcgtattg cttgatgtga tcgatagacc
     9901 tttaggcact tatttagatg ctgagatgac agatatttta gaacaacacc taaatgaaaa
     9961 cggtattgaa gttttgacaa atgcaaaaat cgaagctttt actggtaacg gaaaagttga
    10021 ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta gtcattcaag ctgccggtgt
    10081 gaaggcaaat acagaatggt taaaagggat cgtcgactta gatgaacgtg gctggatcca
    10141 aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag atgctacact
    10201 tgcttattct atcccagcac gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag
    10261 agaagcacgt tatgtagtaa aacatctttt tgaagaagta ccaaacaaac cgttcggcgg
    10321 agtcgtaggt tcctctgcat taagtgtctt tgactatcat tttgcagcaa gcggattgaa
    10381 tagctttaca gcagcaaaag caggagtaga tgtacgagtg tcttattatg aagatacgtt
    10441 acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc aattattctc
    10501 ggatccaatg acccatcaat tgctaggagg agcagtatta tctacctatg atgttacagg
    10561 tcaaggaaat gtattggcac tagcgatcca aaacaaactg actttagaag aactagcaga
    10621 agctgacttc ttcttccagc caggctttga ccgacaatgg agcatgttga atctagccgc
    10681 tcaacaagcg ttaggagaag aaccatttgt agaataagaa aacgaaatat ttgaaaagag
    10741 gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa cagcttctta
    10801 tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttatttt
    10861 caaaaaaggg actgtgacac gatttgtgta ccgaccccca aaagttagac caaaaaatct
    10921 aacttttgga ggtcggtatt ttttatggtt aaatatactt ttgaacttaa attaaagatc
    10981 gttcatgatt atttggatgg caaaggcgat tggaccgtaa aaatccagat caagagattg
    11041 aagcggaaat gcttaaaatt cgggagaagc acaaggatta tggctgttta cggatgacca
    11101 atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc ttgatcaaga
    11161 agctaggaat aaaagtaacg gcatacacgc gtaaatcacg tcgctacaat tcttatcgag
    11221 gaaaagt
//
LOCUS       DAZBWR010000095        10802 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000095 DAZBWR010000000
VERSION     DAZBWR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.098"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..865
                     /locus_tag="ACHGT5_002180"
     CDS             <1..865
                     /locus_tag="ACHGT5_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFN7218150.1"
                     /translation="REKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKI
                     NNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIF
                     SEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAE
                     QYRRSLELSKD"
     gene            937..2058
                     /locus_tag="ACHGT5_002181"
     CDS             937..2058
                     /locus_tag="ACHGT5_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7218151.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2140..3912)
                     /locus_tag="ACHGT5_002182"
     CDS             complement(2140..3912)
                     /locus_tag="ACHGT5_002182"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218152.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3915..5627)
                     /locus_tag="ACHGT5_002183"
     CDS             complement(3915..5627)
                     /locus_tag="ACHGT5_002183"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218153.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5742..6410
                     /locus_tag="ACHGT5_002184"
     CDS             5742..6410
                     /locus_tag="ACHGT5_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN7218154.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6477..7265)
                     /locus_tag="ACHGT5_002185"
     CDS             complement(6477..7265)
                     /locus_tag="ACHGT5_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN7218155.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7348..8820)
                     /locus_tag="ACHGT5_002186"
     CDS             complement(7348..8820)
                     /locus_tag="ACHGT5_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN7218156.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8950..10143)
                     /gene="metK"
                     /locus_tag="ACHGT5_002187"
     CDS             complement(8950..10143)
                     /gene="metK"
                     /locus_tag="ACHGT5_002187"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN7218157.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10141..10220)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10409..10480)
                     /locus_tag="ACHGT5_002188"
     tRNA            complement(10409..10480)
                     /locus_tag="ACHGT5_002188"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10445..10447),aa:Glu,seq:ttc)
     gene            complement(10492..10565)
                     /locus_tag="ACHGT5_002189"
     tRNA            complement(10492..10565)
                     /locus_tag="ACHGT5_002189"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10529..10531),aa:Ile,seq:gat)
     gene            complement(10578..10648)
                     /locus_tag="ACHGT5_002190"
     tRNA            complement(10578..10648)
                     /locus_tag="ACHGT5_002190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10614..10616),aa:Gly,seq:tcc)
     gene            complement(10653..10725)
                     /locus_tag="ACHGT5_002191"
     tRNA            complement(10653..10725)
                     /locus_tag="ACHGT5_002191"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10690..10692),aa:Phe,seq:gaa)
     gene            complement(10729..10801)
                     /locus_tag="ACHGT5_002192"
     tRNA            complement(10729..10801)
                     /locus_tag="ACHGT5_002192"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10766..10768),aa:Asp,seq:gtc)
BASE COUNT         3582 a         2200 c         1796 g         3224 t
ORIGIN      
        1 acgagagaaa aataatttaa agaaaaaaat gctcattcca tttgattcag aagtgaaagg
       61 attactcaat aaactaacta tttctaatat agactcgact agggaaaaaa tcataaaaat
      121 atttgataaa attaataatt tattcaagaa tgacaatgct gaattaagga agggtctttt
      181 agagtttaaa cttcaagaac tagaacttca ttactcttat ttagtgaaaa ttaccgaaga
      241 aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa gaggaaaaag ttcgtagaga
      301 aattgatcgt gaaaagaaac gtattgataa agaacaacgt caattcaatt ctgaaatttc
      361 caaattaatc gcatatatgc aaaaagctaa tgcagatgtg gaaaaagaat tatatgcaaa
      421 taaaattcaa gaactggaag aaaaattaaa agaattagaa gttatacgtg aaaatgtatt
      481 acaaagagaa ttaaatacta gagccggtta tgtgtatgta attagtaata ttgggtcttt
      541 tggtgaagac atatataaaa ttggtatgac tagaagatta gaacctatgg atagaataaa
      601 agaattgagt agtgcatcgg ttccatttga atttgacgtt catgcgatga ttttttcaga
      661 ggacgctccg tctttggaaa ctaaattgca taatcatttt agaaaacaag aagttaataa
      721 aattaatcaa agaaaagaat ttttcaaagt ttcactagac gaaatagaga aagttgtttt
      781 agaaaattat aatggaacag taacatttac aaaattagct aaagctgaac aatatagacg
      841 atctttagaa ttgtcaaaag attaaatata taaaaagcct tcgggctttt tcttttaaca
      901 attaatagga acgtatattc gaaaggaata gaaataatgt ggttagaaaa aataaatgac
      961 aaaaaattta agtacaccga acgttatacg gatcccttaa ctgaaaaaaa gagaaaagtg
     1021 tccgttactt tatctagcaa ttcacgccaa gcttggaatc aagctaactt acttttgaat
     1081 gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac cactaacttt tggagaatta
     1141 aaaactaaat gggatgaaaa atacaagcca actgtaaaag aaagttccta tcgaacaact
     1201 caggtttatc tatcattaat ttcaaaatat ataaaagatg atgtacttgt aaaaaacgtt
     1261 aatagtaatc ttatacaaga tatgctagat tactactatt ttgaaaaaaa tttatcatat
     1321 aattatgtaa tacatttaaa aacttttaca ggaatgattt ttaaattcgc caaccgtcga
     1381 tatggattaa aatataatcc cgtaaatggg gtttctttag caagaaaacc taagacaaac
     1441 gaagaaataa aaaaagaaaa agatggttat ttatcaaaag aagaagttca aatgattgct
     1501 agtaaacaaa aaagttcaca tcaacagtca cgttattctt taattactaa atttttgttt
     1561 ttaactggac tacgttatgg tgaattgata tctttgactg aatctgatta tgatggtaat
     1621 aaaattacgg tttctggtac ttatgattac gagttaaaaa ttaaaacaac aactaaaaat
     1681 acaggttctt atagaaccat tgaattatca agtaatgcta aagaaatcat agatcaatta
     1741 attgaagaaa acaaattaat taagaataat aaagataaat acattttcat ctctaaaaat
     1801 ggtaacccga tatctattca agcatataat caatctttaa gagcagtatc caaagaatta
     1861 aacctagata aaaaagtttc ttctcacatg ttaaggcatt cccacatttc tctacttaca
     1921 gaattaggaa tcccattaaa agccattatg gatcgtgtag gacatgaaga ttcaaaaaca
     1981 acattaaaga tttatactca tacaactaaa aatatgcaaa atcaactagt agaaaaacta
     2041 ggaaaaatag aaatttaaat tgcccctttt ctgcccctta taaaaacaaa caacataaaa
     2101 tatataaaaa gaaccccgtt ataacgaggt tcttttaatt taactttcaa atacaaattg
     2161 attatgataa agttccgcat aaaatccatt ttctgctaat aactcatgat gactgccttc
     2221 ttcaatgact tccccatcac gcaagacaac gattctatct gcattcaaga ttgttttcag
     2281 ccggtgagca atcacaaagc tggttcttcc tttgattgct tcatccattg ctttttggat
     2341 ccttgcttct gttaccgtgt ctacattact tgttgcttca tccaaaatca ataaagatgg
     2401 atttgtgatg atcgttcgag caatactgat caactgcttt tgacctgtac tgaataagtt
     2461 attttcttcg gatatctctg tatcataacc tttttctaac gtcatgatga agtcatgaat
     2521 attcgcttgt tttgctgcat tgatgacttc ttcatctgaa gcgtccggtt tgccgaatgt
     2581 gatattgtct ttgatcgttc cggagaacaa tacagattct tgcaaaacga ttcctacatg
     2641 tctacgtaat gcatcaagat cataatctcg gatatctatt ccatcgatga ctactgaacc
     2701 atcattgaca tcgtaaaaac ggtttagcag attcatgatc gtcgttttcc ccgatcctgt
     2761 tggaccaaca agagcaacca tttctccttt gtccacattg atcgagacat ctttcaagac
     2821 tagtttttct ggagaataac caaaatctac gtgctgcaac gcaagacctt tttcaacacc
     2881 atcaaatgct ttaccgtttt ctggtttgac ttcatctttt tcatcaaata tttcattcaa
     2941 tcgtcttgcc cctgtaatcg ctaattggat catgctgtat ccagatgaaa tctgcatcag
     3001 tggttggtaa tattgttgtg aatactggac gaatgtcaca actaagccta atgccaccga
     3061 acgttccaaa tcaccattta atgctaacca gccgccgaaa aagataacta tcgctgtatt
     3121 gaccagtgac attccttgca tcatcggaaa tagtagacca gaataagctt gtcctttgaa
     3181 ggttgcttcg cgaacacttt gattatgctc taagaatcct tcaatcgttt cttcttgtaa
     3241 gccatttgtg atgatgacac gttgtccgct gattttttca tccatataac catttagttt
     3301 ccccacttca tcttgctgaa gatccacata tttccgtgct ttgcggataa tcaagacagc
     3361 tatcaaaacg gctactggag tggaagcaat tgtagcccaa gctaactcta cattttggcg
     3421 gaacatcatg atcaagatcc cgacaagtaa aacagcattc gtgatgactt gaagcaaagc
     3481 ttggttcaaa ctattttgaa tattatctaa gtcactggta aaacgactta aaatttcacc
     3541 gtcttgatga gaatcaaaga aacggatcgt caatttttcc aatttgttga atagaccgat
     3601 acgcattcgg ttcgttgact tccctactac ttgtgtgaac aaaatactat agataaagtt
     3661 ggctgcacta gctagtacat aaaacagcaa gagtttccat attacagaaa caaatttgct
     3721 tttatcgtct acgccttgca tcaagcctcc tacgtaatta gctaattctt gaaaagcttc
     3781 cccgatgtat tgaggtgcct ttacttgtag ataggtagca atgatcactg tgataaagat
     3841 aaagaaaaag gagagtttgt accgcttcaa atagtgatag aaaaatttac ttgcttttat
     3901 tagatcagtc atacttactc ctccgttcct ttttgagttt caaagatttc ctgatagaca
     3961 cggtttccag ctaccaattc ctcatgggtt ccttcgccga ctaatctgcc ttcatccaat
     4021 actaagatgc ggtctgcttt gacaacagaa gcaatttttt gtgcgatgac aatcgtcgtt
     4081 gttcccttca aatctcgatc gagtgcttct cggaccagct tttcagagcg agcatccagt
     4141 gcactcgtac tgtcatctag gatcaagatc tttggattac cgataacgcc acgagtaatg
     4201 gataaacgtt gcttttgacc accagaaaag ttactgctac gttcttctat tggtgcttcg
     4261 taacgatcag ataacttttc gatgaattct tttgcttgag caatagaaga agcatgttcc
     4321 atctcttttt ctgttgcatt ctttttccct tggcgcaagt tttgagcaat cgttcctgag
     4381 aataaaattg ctttttgcaa aacaaaggaa accgtttgtc ttaaactctt ttcatttact
     4441 tgacgaagat ctacaccacc tacttcaact ttgccttctg tcggatcaaa tagacgaggg
     4501 atcaattgtg ctagtgttga cttcccggca ccagtcgccc cgacgatacc aaccatttct
     4561 ccaggtttga tagaaaagct gatatcttta agcgtatctg tatcctcacc aggataacga
     4621 aaagaaacat ggtcaaaagt gacagaaccc gtcaactctt gttctggaac gtctaaataa
     4681 gtaagatctg gttcagcatc taaaatttcc gcaatccgct tcagcgatac ggctgctctt
     4741 gaagtcatca tcatcatcat tccgccaata atgattgcca tcataatctg catcaagtag
     4801 ttcatgaatg aagcaattcc accaattaac gtaggatcat ctttaacaag atcgctcaca
     4861 aaaaagatcg ccccaaccac agccaaattg gcagccagca tgaaggacgg gatcatcaca
     4921 gaaaataatg taccgactat gatgttgtgt tttgttaagt cctcacttac tttgctgaat
     4981 gacttcaatt ggttttcttc ttgtacaaaa gatttcacta cacgaatccc tagtaagttt
     5041 tcttttgcaa tgctattcac acgatcaatc agtttttgaa tgatcatgaa gtgcttgccc
     5101 atccttgtaa atgaaagtgc cgtaatcaga aataccgtaa cgataagcgc tacgatgatc
     5161 caccataatt gcggcatcgt gatcatagct aaaatgaagg caccaataaa taagaatgga
     5221 attcgaaaaa gtgattgcaa agaaatcatc actaaatttt ggatttgcgt gatatcattt
     5281 gttagacgaa caacaagatt tccagcagag aactgttcga tatttccaaa tgaaaatgtc
     5341 tggatctttc gaaatgcttc ttctcggata tctgcactta cgccttgagc tacttttgca
     5401 gagaaaatcg tgttcgttac acctgcggct aatcctagca aagccagact gatcaggtaa
     5461 ataccaattg ttttcatttc atcattatct tctgtaataa tagcttccaa tacttgctgt
     5521 agtaacttag gttgccataa tgctgctgca accatgactg ctacggacag caaggaaata
     5581 aaaacagata ctttatattt tttcgcatgt cgtaagatta atttcattcg ttgattacac
     5641 ccctttgttt aaattgacag gactaattta acactgtaaa attagaccga ctaacaattt
     5701 accatgttta aaaagataat gcaatacacg aggttgataa aatggaaata aagctttctc
     5761 aagcaatcat cattcaggcc gcctttgata tcctggctga aacacaagct ctttcgcagc
     5821 tttccatgcg aaacatcgca aacagaattc atgttcaagc acctgcttta tattggtatt
     5881 tcaaaaacaa acaacagctc ttgcaaaaaa tggctgaaac aatggaagaa gaattagcgc
     5941 ttccagatcc aactttgcct tggaatgaac agttgctgca gtatatggaa aattactatg
     6001 acctttatac acgtttccct tgtggggcag aattagaaat caatactgtg cccgcttttc
     6061 cgagtcgatt agaacatttg gagcaaatga tccaattact tgttcgtgaa ggattttcag
     6121 tcacccaaag tcatcaggcc attgcctctc tccacaattt attgattgga cagttaatgg
     6181 atcagcagca tgaacagcag cttcgacaaa aaattatgga cggaaatgaa tttttgaaag
     6241 aaacagttct atttatgcgt aattatgtag ttgaaaatca attagaggga tttaaagaaa
     6301 gtatgcagtc ccatgcagaa ttgaatgaaa aagcactctt tctaacgagt gcacaaacct
     6361 atattgaagg cttatgccaa caaaataaaa gaagagaaaa cgagcagtaa aaaagctcct
     6421 atagagatca ctatgtttgt gatctctata ggagctatct ttttaagact ctttttttat
     6481 ttttcttcca ttagccacgc caatgcacgt tgaatccatt ggtcccagaa gatccagtta
     6541 tgatcgccag gtccttgttc aaaggtgacc gccaagcctt gttcctgcat agcagatacc
     6601 atttcctgat ttgcctgata aagaaaatca gattccccac aagccatata aaatcttgga
     6661 actggttttc cgctttttac tagttctttc aataagtatt gcggatcatt ttttgaacct
     6721 tcgatttgat ccaatggtcc aaagatccct tcccaaaaag atgctttacg aaccatcaaa
     6781 agatcatcca ttcgatttcc tacagaaaca gcgcctgaaa gagagattac tgctgcgaaa
     6841 ttttctgatt ttgcaaggcc tagttttaac gcgccatatc cgcccatcga taatccagcg
     6901 gcatatgttt tttctctttt tcttgttaat tgcgggaata attcatgaca aatctcaggt
     6961 agctcttctg caatgaatgt ccaatacttc atgtcataaa ctgtatctgt gtaccagcca
     7021 agatcagtag atggcatgat gacagctaat ccgtagtttg ctacataacg ttcaatcgac
     7081 gtacgtcttt cccatacact atgattgcca cccattccat gaagtaaata taaaacaggt
     7141 atatccgttg tctcttgctc tgaactggtg ccaatcacct tttttgttgt ttgcggtagt
     7201 atcacattta gattgacttc catttctaac acattcgaat aaatatttgc ttgaataaat
     7261 gccatatttt cgccttcctt agtaagaagt tatttttgaa taactttaaa tttgaatgat
     7321 tcggctacgt acatatctta accgaaacta ctgttttatt ttaccacttt tttcatgaac
     7381 gaaaagcgtt aagaccaaag caatgatcac gagtaacaag ccaactagat aaacggaatg
     7441 taacccatca taaagaattt ttcttaatgg taacaaaata tccgcatcaa tatatttagc
     7501 cgtttgtgga ttcaccagtt tattcatgat atctgcatca tttgttaaat ttgctgctgc
     7561 aagttctcgt ttagttaccc catttatcaa taaaccaaaa atagaaacca tgactgtttg
     7621 accaattgtt cgaactaacg tattaaaaga agttgctaca ccaagctggt cacgagatac
     7681 actattttgt gcactgacag tcgaggtagt catggtcaca cctaagccta taccgatgac
     7741 cgttgatata gcaaaaaacc ataaatagtt acttctgaaa ggcataagat acaaccaagc
     7801 gcccccaata gctgcgatta gtagtccaat tctcaagacc caatgagttt tcatttgagt
     7861 catccacttg cttgcaagga aagaaccgac catccagact agtgataatg gtgctaatac
     7921 cagcccacca attgctgcat tcagccctaa tacaccttgc atccacatag ggatataaac
     7981 atcgattccc atcaaaaaac cactagccaa tgctgcagcc aagttgacca caacaaatag
     8041 tggatttttg aataaatgca tcatgatgac aggatcttta gctctttttt cggcaaaaac
     8101 aaaaatacct aacattacga tactacctag cagcaaagca gaaacaagcg gactcattcc
     8161 ttcgtctcct aataattgga accctaacag caatgagaca agcatggcca tcaaactaaa
     8221 actaccaaaa atatccattg gtttagactc atgtatgatt tttggttcat ttaaatatag
     8281 ccaaatcaaa ataattaata gaattccgat cggcacattg ataaaaaaga tccagtgcca
     8341 gctgatcgtt tcaacgatga atcctcccgc aagcggtcca aaaacactgg caatccccca
     8401 ggctgaacta ttcaaaccta aaacttttgc tcgcttttca atagaataaa gatcgccgat
     8461 gatcgtcaaa gctactggca tcattgcccc agcacccatc ccttgaatcg cacgagcagc
     8521 aatcaaccca atcatgcttt gagataaacc gcaaaataac gaaccgataa taaacaataa
     8581 tgtcccaaaa ataaagattg gtttccgtcc cactttatcc gcaagttttc cgtaaatcgg
     8641 agtaagcata gcgtttgtca gcaaataaat cgaaaatacc cagttcatga tttccattcc
     8701 atgtaatgaa ccgacaatcg ttggcatagc ggttgttacg attgtacctt caatagccgt
     8761 catgaacgtt gcaatgaaaa tactcactgt gaccaaagta acatttgttt cttttttcat
     8821 cttattcctc cctttcatta tgacaggatc taaaaataga taaataatta ctatcttttt
     8881 caagaggtta actgccataa aaaaagctgg aacaaccacc acttggcgat cgttccagct
     8941 gaatataact tattttgcta aacttgcttt caacgcatct actttgtccg tatgttccca
     9001 aggcaaatct acatctgtac gaccaaaatg accgtaagca gctgtttgtt tgtaaatcgg
     9061 acgacgcaaa tcaagcatct cgatgattcc tgctggccga agatcgaagt tttctctcac
     9121 tgcagcaatc agttcttctt ccggaaccgt tccagtaccg aaagtattga ttgagatcga
     9181 aacaggttga gcaacaccaa tcgcataagc tagctgtact tctgctttgc gagcaagccc
     9241 tgctgcaaca atgtttttag caatataacg tgcagcatag ctggcagaac ggtcaacttt
     9301 tgtcgcatct ttaccagaaa aagcaccgcc accatgacga gcataaccgc catacgtatc
     9361 aacaatgatt tttcttcctg ttaatccggc atccccttga ggaccaccaa tgacaaatcg
     9421 gccagtcgga ttgatataat atttcgtttg atcatctaat aattcagctg gaataacttc
     9481 cttcactact ttttcgatga catcatgacg aattgtttca ttatctactg catcatcatg
     9541 ctgtgttgaa ataacgattg tatctacgcg ttccggttgt ccttgatcat catattcaac
     9601 cgttacttga gattttgcat ctggacgtaa atacgttaat tcatttgatt tacgcaaatc
     9661 tgccaagcgt cgtaccagac gatgactcaa agcgattggt aaaggcatca attctggtgt
     9721 ttcatctaca gcaaacccaa acattaaacc ttggtcacca gcaccaatct catctaatac
     9781 gtctttttta tcttcatccc ggatctcaag tgcttcatca actccttgag caatatcagg
     9841 agattgttca tcaatcgcaa ccaatacagc tgctgtatct ccatcaaatc cgaattttgc
     9901 acgtgtataa ccaatttctt ttattgtttc ccgtacgact ttttgaatat ctacatatgc
     9961 cgtcgtagat atttctccaa atactaaaac aagaccagtt gttacagacg tttcacacgc
    10021 tactcgtgct gttggatctt gttttaaaat tgcatccaag attgcatcac tgatttggtc
    10081 agcaatttta tctggatgtc cttcagaaac agattctgat gtaaataagt gtctttctac
    10141 cattataatg attcccccta gttaaattcg gttacaaggc atctccgtcc tgcggatcct
    10201 ttcgggaact tgcaacgaac caagtatagc agataaaaca gttttctgct cgtatttttt
    10261 catttttttg cttaaaaaat ataaaaatat tttacattag aactgtccaa aaaagcttag
    10321 cacaatcatc cataaagata actctgctaa gctttttaaa atcattcttt cagataaaaa
    10381 gaaaaagata ctcaaaaagt atcttccatg acccgtacgg gattcgaacc cgtgttaccg
    10441 ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aataaaataa atgggcctaa
    10501 atggactcga accatcgacc tcacgcttat caggcgtgcg ctctaaccag ctgagctata
    10561 ggcccataaa aactaaaagc gggtgacgag aatcgaactc gcgacaacag cttggaaggc
    10621 tgtggtttta ccactaaact acacccgcat aatggcgcgg gacagaatcg aactgccgac
    10681 acatggagct tcaatccatt gctctaccaa ctgagctacc gagccaaacg gttccgacgg
    10741 gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac
    10801 ca
//
LOCUS       DAZBWR010000096        10665 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000096 DAZBWR010000000
VERSION     DAZBWR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.099"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHGT5_002193"
     CDS             33..656
                     /locus_tag="ACHGT5_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218158.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHGT5_002194"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHGT5_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN7218159.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHGT5_002195"
     CDS             2152..3033
                     /locus_tag="ACHGT5_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7218160.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHGT5_002196"
     CDS             3036..3806
                     /locus_tag="ACHGT5_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218161.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHGT5_002197"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHGT5_002197"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN7218162.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHGT5_002198"
     CDS             5117..6280
                     /locus_tag="ACHGT5_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN7218163.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHGT5_002199"
     CDS             6397..6807
                     /locus_tag="ACHGT5_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7218164.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHGT5_002200"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHGT5_002200"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN7218165.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHGT5_002201"
     CDS             9380..9664
                     /locus_tag="ACHGT5_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218166.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHGT5_002202"
     CDS             9824..10060
                     /locus_tag="ACHGT5_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN7218167.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHGT5_002203"
     CDS             10057..10455
                     /locus_tag="ACHGT5_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN7218168.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3726 a         1773 c         2254 g         2912 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttag
//
LOCUS       DAZBWR010000097        10662 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000097 DAZBWR010000000
VERSION     DAZBWR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.100"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..545
                     /locus_tag="ACHGT5_002204"
     CDS             3..545
                     /locus_tag="ACHGT5_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218169.1"
                     /translation="MIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLL
                     SILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKH
                     RCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLE
                     YAFSFAREDEDNPKTYDILA"
     gene            complement(606..971)
                     /locus_tag="ACHGT5_002205"
     CDS             complement(606..971)
                     /locus_tag="ACHGT5_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN7218170.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1047..1292)
                     /locus_tag="ACHGT5_002206"
     CDS             complement(1047..1292)
                     /locus_tag="ACHGT5_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7218171.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1362..1616)
                     /locus_tag="ACHGT5_002207"
     CDS             complement(1362..1616)
                     /locus_tag="ACHGT5_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218172.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1780..2103)
                     /locus_tag="ACHGT5_002208"
     CDS             complement(1780..2103)
                     /locus_tag="ACHGT5_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218173.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2290..2363
                     /locus_tag="ACHGT5_002209"
     tRNA            2290..2363
                     /locus_tag="ACHGT5_002209"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2324..2326,aa:Ile,seq:cat)
     gene            complement(2748..3245)
                     /locus_tag="ACHGT5_002210"
     CDS             complement(2748..3245)
                     /locus_tag="ACHGT5_002210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN7218174.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3285..4094)
                     /locus_tag="ACHGT5_002211"
     CDS             complement(3285..4094)
                     /locus_tag="ACHGT5_002211"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7218175.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4428..5285
                     /locus_tag="ACHGT5_002212"
     CDS             4428..5285
                     /locus_tag="ACHGT5_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN7218176.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5278..6180
                     /locus_tag="ACHGT5_002213"
     CDS             5278..6180
                     /locus_tag="ACHGT5_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN7218177.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6213..6563
                     /locus_tag="ACHGT5_002214"
     CDS             6213..6563
                     /locus_tag="ACHGT5_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN7218178.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6612..7535
                     /locus_tag="ACHGT5_002215"
     CDS             6612..7535
                     /locus_tag="ACHGT5_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN7218179.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7613..8506
                     /locus_tag="ACHGT5_002216"
     CDS             7613..8506
                     /locus_tag="ACHGT5_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN7218180.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8544..9107
                     /gene="amaP"
                     /locus_tag="ACHGT5_002217"
     CDS             8544..9107
                     /gene="amaP"
                     /locus_tag="ACHGT5_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN7218181.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9121..9309
                     /locus_tag="ACHGT5_002218"
     CDS             9121..9309
                     /locus_tag="ACHGT5_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN7218182.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9322..10224
                     /locus_tag="ACHGT5_002219"
     CDS             9322..10224
                     /locus_tag="ACHGT5_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN7218183.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10256..10498
                     /locus_tag="ACHGT5_002220"
     CDS             10256..10498
                     /locus_tag="ACHGT5_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN7218184.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         3760 a         1838 c         2036 g         3028 t
ORIGIN      
        1 gcataatagt tataagcttt tttaaagaat atttagaaat tattaatttt cgtctgaaaa
       61 caaaaaaaga aagcgttatt attttagtct attttcaggg ggggaacctt atgataaaaa
      121 aactattttt actatcaata ttaacgctag caatggcagg ttgttctgac tctggcttag
      181 caactgaact gatcacaaaa tcaactgaag ccacgacaga ggagttaaaa tgtggaaacg
      241 atcacagtga ctacagaggc ttaaatcgac gatgcatggg aatattaggc agaagcaatt
      301 gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag caacagaaaa atcaaaagaa
      361 agatcaaaag aagcaatctg tgctgtgcat cgtatacaga cgtcgaagat gcggtacttt
      421 taaaccacgt caggagatgt tcatttatgt ctagggaagt tggcgatgaa ccaaaactag
      481 agtacgcttt tagttttgca cgtgaggatg aagataaccc taaaacttac gacattttag
      541 cttgatctaa gatggttaaa gctttttaat gaagtaagag gagatagaag gcttttctag
      601 ttatattagg taagataaat tcctttttgc tccaaatttg ctaaacattc tgctagataa
      661 acttcatcgt gttccggata aatcggcacg ttgcgctcta cagtaggtaa atcaaaaaat
      721 gcttcgcatg atttgccacg atacatagga tttttccact ctggtgctgg gctgtaacca
      781 cgtttttcca tttcatccat gacaagctga tgatattgga ataatctata aggagaatag
      841 tgaaaaacat aatccacagt agcatgtttc tttccccagc catttcctct taatgcacag
      901 cattcccgat gttgtcctaa aagctgttga cgaggaagct ttgaaatcaa tttttcatgc
      961 cataatcgca tagaaatgtc tcctttatat atagattgtt aaccttagac gttaaatgaa
     1021 aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc ttgctgtttc tgagcgacca
     1081 attaggtaat caacggaaat atcgtaaaag tcagcgatat agattaattc aatgatgctt
     1141 ggaatttctt ctccttcttc gtacttacga tatttgaata aaggaatact taacgcgcta
     1201 gaaacagcta cttgagataa agatcggcca gttcttaaga aattcagacg acaacgtaaa
     1261 gtatccagta aaatagcttc ttcgatactc atttaatcac ctttcattat agtagaatag
     1321 ttataatgac tatagaaagc tacacatgca atcaatagta ctcaatgttt tttatttaaa
     1381 cgtatcaaat aaagaatgac gatcaataaa gacaaaacca gtcctgataa ttcttctata
     1441 aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa gtagatagcc
     1501 ggaagagtca aaatagctaa ataaaaccgt tctttgttcc aattcataag ataaacaata
     1561 cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa
     1621 cggactcctc aacgattcat ataataaaag ataacatgtt attgcataca tcacaaatga
     1681 aatgctagga aaataaaaga agaatagatc aatgaaaaat gaaaaagcag aacagttttc
     1741 tgctgataag caggaactgt tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg
     1801 attgatatct aagtaattca atacatataa aaagaggacg atcgcattca ctaaaaagga
     1861 aagaatgatg atcccatagc gaatggaaac atatttaaca aatacttttg tttgaatacg
     1921 ggcatatctt tttcctttat agctgagcat catgacaaga agcaaaaggg taatcacacc
     1981 ttgaaaaatc attccaataa acagaattcc tgaggtttga taatttaatt ggagaaaata
     2041 agctgctgcc gcaactaaat cgaataaaat cgcaataaat ggcaaaataa tcactcctaa
     2101 cattgagaat aaataaaaga ataacatttt ttctaaaata agcaaataaa aattaaagta
     2161 acaaaatagt tatgacacaa gtaaaagaaa taagaataac aatattattt tacttatatt
     2221 ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa aagtaacaaa aataaatttt
     2281 atttatgatg tacctttagc tcagttggtt agagcagacg gctcataacc gtccggtcgt
     2341 aggttcgagt cctacaaggt acataagagg ttgtgaaaaa gctgccctgc atcaagtaat
     2401 aagaacagcc aagtaaaaac agcttcttat gtttttactt ggctgttctt attactgcag
     2461 atgcaagctt ttgaataccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt
     2521 attagcttaa caaacggaaa aatagttcct catattttgc cgtttgttaa gctaataccg
     2581 agggtcagct gattgaacac catttaagaa agaggtcgtg agcctgccgt ttcactctgt
     2641 gtcataaaaa agccaatcta ctttgataga aaaagcacgt aggactaaaa cagttctgcg
     2701 tgcttttttt attgtgtgtt aaatatcgta aatgaagata gatattttta tgattcgacg
     2761 atagagacgt tgaaatcttt caacagctct aaagcggatt ctccgtgttt tctactggag
     2821 actaacattg gatcaacaaa aaagtctgct tcaggaaaag cactctgcag gcctatcaga
     2881 ttagagagta cacacaagtt tgtttcaatc ccgactactt cgatctcttt ttgttcttct
     2941 ttgctttcaa ataaagtttc ctggatttca gctaaagttt caggtggaat cgtaaagtaa
     3001 tactttttaa tcactcgttc attttcttgt ggagaaagaa gagggatcaa ttgcaaatct
     3061 tctcgttctt cttctttgtc cttcagttca actggtatat cccgtgtgaa gagaatatat
     3121 tcattatttt ggcgtgcttt agctaatctt tgagcaatac gttctgccaa ttgatctgac
     3181 tctggaatat aaaattcgct gtttggatca agaatatgat tttgcatatc aataataact
     3241 aacattttct ttcctcctac attcatatcg tcattttatt ctttttaata gaagtttttt
     3301 aaaatataag caacaccagc ttcttcattc gtcagtgtta cttggtttgc aatttctttt
     3361 acttgtcgct gtgcattccc catcgcaata ctcaacccgc tgtaattcaa catagcaatg
     3421 tcgttttctt gatcgccaaa agccatgatg tcactcgatt cgattccaag gtaatccgcc
     3481 agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg tgatttctaa gaaatgcgga
     3541 tcagagaata ctgattggat accatctgct tgcagatctc tttgtagctt ctgtagtttt
     3601 tcttcactct tatctgttaa tgctaattta agatagctaa gagaaggatc ttccaacata
     3661 tttaaaattt gatcaaaaga atggttctgt aaattgaaat gttcataata agggtcatct
     3721 ttgactagat ttgattcatc gaatgtcagc tgatcatctt gggtttctcg gctagcaatg
     3781 atggatattt tcgcttcttg agcatagaag aaggcttttc tcagtgtatc gctatctatt
     3841 tttgaacgtc tgagaacttt cgccattttt ccatccttca tttcagcaat cagtgaacca
     3901 ttggaagaaa tcaaatagcc accgattcct aattcggaaa aaatatcttt agcagaaatg
     3961 ctgttccttc cggtagcgac ggttacgatt ccgccatttt catcaaattt ccgaagtgct
     4021 tggatatttt cttctggtat ctttccattt cgatcaagca aggtaccgtc taaatcaatc
     4081 gctgctagtt tcattttatc ttcctacttt ctttttacat atctattttc ctgttacctt
     4141 aagattaaat gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat gaattttttc
     4201 aaaaaacatt cttaagaaaa aggtggctat atatagtagt ttttggtcaa tacctttgaa
     4261 taaaaagcca tgaaaaacga atagttagcg cttaccgatg cgttttgtga taaaaaaatt
     4321 ttaaaatatt caatgaaaac aaagcatata ttttgcttta aaaccaaatg aggaatattc
     4381 tagatgtaga acaggttaga aaaagattag aaaaaggagt gattttgatg gagaagagat
     4441 acggcgtagt tcctgtggta actgcttcag acgaaaatta tgctccttac ttgagcgtga
     4501 tgatcgcaac tgcattagaa aattgcaata aaacaagacg cataaaattt tatgtgattg
     4561 acgatggtct atcagaatat agtaaagaag gattggaaga aacggtaaat aaatattcaa
     4621 gtaatgcaag tatccaattc ctgacagtgg aaaaagatat ctacgaagac ttcctagtca
     4681 gtgaccatat cacaactacc gcttacttaa gaatttcttt accaaactta ttagctaaag
     4741 aggattataa gaaagtcctt tacttagatt cagatgtgtt agtactagac gatatcgtaa
     4801 aactatatga tgagccgtta aatggaaaaa caatcggcgc tattatcgat ccaggccaag
     4861 tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt gtacttcaat tcaggagtga
     4921 tggtgattga cattgatcaa tggaataaaa aagaaattac agaaaaaact atccattacc
     4981 tcagtgaaaa tggcgatcga atcatctatc acgaccaaga tgctttgaat gctgtacttt
     5041 atgaagattg ggaacaactt catccaaaat ggaacatgca gacttcgctg atttttgaac
     5101 gtcatccagc accaaatgaa aaatatgaac gtcagtataa agaaggaaat gaaaaacctt
     5161 cgatcgttca ctttacagga catgataaac cttggaatac gttgaaagat catccgtata
     5221 ccaatttata cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc gagatcaatg
     5281 aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc acatttagct
     5341 gcattatttg tctcagtatt agataattgc aacccgtcta aatttgttcg gttctatgtg
     5401 attgacgatg atatcgattt tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca
     5461 cgaatgaact cagatgtgga attcttgaaa atcaataaag aattctttac aaatgtggta
     5521 atcagcgatc gtattccaga aacagcttat tatcgtattg cgattccgga attgttccgc
     5581 ggcacagaag ttgaacgtat cttatatatg gattgcgaca tgatcgcttt acaagatatc
     5641 tctaaattat ggcgtcttga cttcggtgat tcaatcgtag cagctgttga agacgctggg
     5701 ttccatcaac gactggaaaa aatgaaaatt tcagctaaat caatgcgtta tttcaactca
     5761 ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca aaaagtatta
     5821 gatttcatcg aacataatcc agaaaaatta cgattccatg accaagatgc attaaatgct
     5881 atcttacatg accgttggtt accacttcat ccgcgttgga atgcacaagg ctatatcatg
     5941 gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga aactcgcaac
     6001 aatccttaca ttatccattt ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg
     6061 ccaacgaaga aatattacga aaaatatgca gggatgacgg ctttccgttg tgtagctaaa
     6121 ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa
     6181 tagaagacaa caactgaaga gggggagtag gaatggaaat tacaatgtct aaaaatgcag
     6241 tagagacgtt aattgaaaag gttggggaaa atacaaaaat cgcactcgcg cttatcaatg
     6301 attccgatcc atttctaagg gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca
     6361 ttccttttgt ttcggaattt ggagaatatg cgactaaaat cgagcatcca ttattggaca
     6421 tctatacatc taaattagag caaaattatt tcggtaaacg attgaacatg gacttcaaca
     6481 aacagcttga cagcttttca ttagaaaatg aaatcgcagt actcgattat aatatcaaat
     6541 taaagaactg cttcttcagc taatagtttc gagaaagtcc aaaaaggagg tatacattga
     6601 gatactactt tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa aaatataatt
     6661 ggctcgtact tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc
     6721 ttcctgatga tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc
     6781 tttatgagac gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag
     6841 ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga
     6901 ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc
     6961 atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg
     7021 tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa
     7081 caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg
     7141 atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca
     7201 tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag
     7261 aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga
     7321 tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt
     7381 ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa
     7441 gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag
     7501 gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa
     7561 acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga
     7621 accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca
     7681 ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta
     7741 tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat
     7801 cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt
     7861 cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagttg caggacgtaa
     7921 gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa
     7981 agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc
     8041 ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat
     8101 catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat
     8161 cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc
     8221 aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg
     8281 aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg
     8341 acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca
     8401 gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac
     8461 agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt
     8521 cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa
     8581 cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc
     8641 cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg
     8701 tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt
     8761 tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg
     8821 ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt
     8881 caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt
     8941 tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg
     9001 aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct
     9061 ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg gggaccaaaa
     9121 atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact tggattgata
     9181 ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt gatcatggca
     9241 gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga tcactatttt
     9301 aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa taaaatgaac
     9361 aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc aaatggtgtg
     9421 aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt agtccctaac
     9481 gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc tcatcatcca
     9541 ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc taaacctgtg
     9601 gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag ttctgaccac
     9661 acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa gcatgattct
     9721 gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt gcaaaaaatc
     9781 atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg tttcttctct
     9841 aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga aacagaagtc
     9901 ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa agatatcgaa
     9961 aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat gacacattta
    10021 gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa
    10081 gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg tgaatttgct
    10141 tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa
    10201 aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac gaaacatggg
    10261 atttatttgg tcacttatcg tcggaggaat tatcggagca attgcaggag caatcacgaa
    10321 taaaggttct tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg gatcagcact
    10381 tggtcaagca ttattaggtt cttggggacc aagtattgca ggaatggcaa tcgtgccatc
    10441 attgattggt gccatcattt tagtactaat cgtatcattc tttgctcgaa aactgtaaaa
    10501 agacttttaa aaagaggtcg tgaaaaagct gttcttcatc aagtaataag aacagccaaa
    10561 caaaaatagc ttctcatatt tttcatttgg ttgggcatat tatcgaagat gcaagctttt
    10621 gaacactgtt tatacggaat aagaggctgt gacaagactt tt
//
LOCUS       DAZBWR010000098        10634 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000098 DAZBWR010000000
VERSION     DAZBWR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.101"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACHGT5_002221"
     CDS             complement(51..632)
                     /locus_tag="ACHGT5_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN7218185.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACHGT5_002222"
     CDS             complement(638..1897)
                     /locus_tag="ACHGT5_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN7218186.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACHGT5_002223"
     CDS             complement(1899..2945)
                     /locus_tag="ACHGT5_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218187.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACHGT5_002224"
     CDS             complement(3180..3455)
                     /locus_tag="ACHGT5_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN7218188.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHGT5_002225"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHGT5_002225"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN7218189.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHGT5_002226"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHGT5_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN7218190.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHGT5_002227"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHGT5_002227"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN7218191.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACHGT5_002228"
     CDS             complement(7283..7918)
                     /locus_tag="ACHGT5_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN7218192.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACHGT5_002229"
     CDS             complement(7984..9387)
                     /locus_tag="ACHGT5_002229"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN7218193.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACHGT5_002230"
     CDS             complement(9374..10405)
                     /locus_tag="ACHGT5_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218194.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..>10634
                     /locus_tag="ACHGT5_002231"
     CDS             10436..>10634
                     /locus_tag="ACHGT5_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN7218195.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRA"
BASE COUNT         2955 a         2268 c         1802 g         3609 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcga
//
LOCUS       DAZBWR010000099        10401 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000099 DAZBWR010000000
VERSION     DAZBWR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.102"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1958)
                     /locus_tag="ACHGT5_002232"
     CDS             complement(372..1958)
                     /locus_tag="ACHGT5_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN7218196.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            2209..2433
                     /locus_tag="ACHGT5_002233"
     CDS             2209..2433
                     /locus_tag="ACHGT5_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN7218197.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2575..4326
                     /locus_tag="ACHGT5_002234"
     CDS             2575..4326
                     /locus_tag="ACHGT5_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN7218198.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            4326..6110
                     /locus_tag="ACHGT5_002235"
     CDS             4326..6110
                     /locus_tag="ACHGT5_002235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218199.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6568..7419
                     /gene="licT"
                     /locus_tag="ACHGT5_002236"
     CDS             6568..7419
                     /gene="licT"
                     /locus_tag="ACHGT5_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN7218200.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            7516..9402
                     /locus_tag="ACHGT5_002237"
     CDS             7516..9402
                     /locus_tag="ACHGT5_002237"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN7218201.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9443..10345)
                     /locus_tag="ACHGT5_002238"
     CDS             complement(9443..10345)
                     /locus_tag="ACHGT5_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN7218202.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         3278 a         1866 c         2190 g         3067 t
ORIGIN      
        1 ttgattttgt cgctttcgtt tatgagctat ttttcttaca ccttttactt gactaatcgt
       61 aggaaaggga actgtgacaa aagtcttgtc gcagttcctt tttccgaata aacggtgctc
      121 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataaaaa gctgttttta
      181 cttggctgtt cttattactt gatgcaaggc agctttttca caacctctga tacacggtgt
      241 tccagaagta acttcttcgg aaataagccg aaatttcaca aaaatttgaa aagcaatttt
      301 cttaaattcc ttcttatttc ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa
      361 agaatcagtt tctactcgat ttcttctatc gtttcctgca aacgctgttc atattttttg
      421 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
      481 acataagcga tttgatcagc caatgctagt tgagctgagg tggcttgctc gccgaagaaa
      541 ctttcgattt gacctggtaa tcctcgtaaa ttcaagtaag tcagactaat cacagagaac
      601 cagccaagta gttttacaac aaatgaattt ttgaatcggc tccccatctc cgcttggcta
      661 ttggtcatca tcagtaaagg aatcatcgag aatggcaatg caaaagccag aaaaacttgg
      721 gattcattca tcaagttatt caaagcggta tgttcttcga tcgttccttt tgtacttgta
      781 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgactag tcttcttagc
      841 caaattggga tacgcatatg gatgaagcct tccatgatga cttgaccggt caacgtacca
      901 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt agacaagata
      961 ccagattttg ctacagcaat caagatacca ttgctcatag ctgatggatc agataaagct
     1021 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaatac agccacaccc
     1081 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
     1141 gtagaaaagc gtaccgcttg tgctacatct tcttcgtctt ggtgatcgat cttccgagtt
     1201 tgagaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
     1261 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg
     1321 ttagggataa atccggcaaa caaactgtgc caatcagggt tagacaagcc aacttgatag
     1381 acgaaaacga ataaaatgac gaaaatcaag cagacaacca aagcttcgat tttacgaaac
     1441 ccgacttttg tcagcaataa aaggacgaaa acatctaaca cagtgataaa tacggcaatt
     1501 accaatggaa tatcaaaaag caggtagagg gcgattgctg caccaatgac ttctgcgatg
     1561 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacccaa tgtcttactt
     1621 gtccgtgctc ggattgcttg ggccaaatcc atttgggtca cgattcctaa ttttgctgcc
     1681 atatattgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
     1741 tggaaatttt gtccaccagt gatcgaagtg gaccagttac ctggatccat ataaccaact
     1801 gctactaatg cacctggtcc agagtaagca agaagtgttt taaaaaaact ttttcccttg
     1861 ggtacatcca ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
     1921 cgatgcttat gaaattgttt gttggattga ttattcaaga aaaaaccacc tttcctattt
     1981 atctacgatt acattctaca cctcagaaag aaaaaattaa agaaaaagat ttggtatgcc
     2041 taattttttt tcgtggcgtg tcttgctggt taatgaaaga aattttgttc ttctctaaaa
     2101 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
     2161 taaaataaat cattgagagc aattcggaaa tagaaaggag agatttatat ggtatcaaca
     2221 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
     2281 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
     2341 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
     2401 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaaagact ttttgcgggc
     2461 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagac tattcagcaa
     2521 aaaagacaag cacagacaat cctatgctct tgataaagac aaaggaggag taaaatgtcg
     2581 atttttagaa aattaggttg gttttttcga caggaaaaga aacattattt aattgggatt
     2641 ttctccctat tcgcagtggc cttggtacag ttagctcctc caaaagtgat tgggatcatc
     2701 attgatgaga tcgccgataa aaatttatca ataaagatca tttcctggtg gatcttatta
     2761 ttgatccttg cagctctctt acaatatctt tttcgttata tctggcggac gaatatttgg
     2821 ggaagtgcag ctcggttgga gaaagaactt cggcagcagc tattcaaaca ttttacagaa
     2881 atggacaatc tcttttacca aaaatatcgg actggagatt tgatggctca tgcaacgaat
     2941 gatttgaatg caatccaaaa tgtagcagga gctggtatct tgacctttgc agattcagtg
     3001 atcacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
     3061 attgcgctga ttcctctgcc tttactagcc gttacttcac gagtgctggg gtcaaaactc
     3121 cacgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
     3181 agtattactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctcgcggat
     3241 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
     3301 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
     3361 ggtggaaagt atgtcttgga aggcacgatc tcaatcgggc agatggtctc atttattagc
     3421 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
     3481 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
     3541 gaacaactgg gtgcagtgca gactccggca aaaggcgagc tgaatgtagc tatttcctct
     3601 tttaactatc cgaaagaaga atcaccagca ttagaacaaa tcaaatttgc gatccaagaa
     3661 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacaat catcaaatta
     3721 ctgatgcgtg aatacgatga ttatcaagga aaaattcaat ttggcggatg ttcgatcaaa
     3781 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcaaga tcacttcttg
     3841 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
     3901 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
     3961 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
     4021 tcgattgctc gagcattgat caaaaatcct gaattgctga tcttagatga tgcactttct
     4081 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
     4141 aaaacaacca tcattgcagc tcaccgttta agcagcgtga tgcatgcaaa agaaatcatt
     4201 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcattaggc
     4261 ggctggtatg aacgaatgtg ggagaaacag aaattagaag cgaagataga aggaggcgag
     4321 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
     4381 agaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
     4441 ctatcttatt tgcgtttgcc ctgtctgtga tcaatgtcct attgccaagg atcatccaaa
     4501 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
     4561 ctggtatgta cttgtttgga gtcatagtca aaagtatcat ttggtttttc caatggtatt
     4621 tgtattccat ggcttcattg aagacctatc agtatgttcg ggtaagatta tttgaaaagc
     4681 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcgact gtttctcgag
     4741 tgacaaatga cacagaaacg ctatttgaat tttggtatgt ctttttgatg gtattgactg
     4801 ggatctttgc agtcgtttct tccttcattg ccatgtttca aatcaatgga gaaattgcct
     4861 tgtggacttt ggtgttcttg ccgattttag gaatcgtcat ctggtattac cagaaattca
     4921 gttctcgtat ttaccggaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
     4981 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
     5041 aatttgaaca agtgaacaac gagtatttag acacgcgcta tgcgatgatc aaaacaaatt
     5101 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggcgttga
     5161 ctttgtttgg gatcgatgcg ctgcattctc ccgtagaagt tggactgatc tatgcattta
     5221 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcacct
     5281 ttacagatgg aatggttgcc ggtagtcgta ttttgaaaat catggataca gaagaatatg
     5341 caccgcaaca aaatcctgga gcagatcaag cgattacttc aggtaagatc gagtttcgca
     5401 atgtaagctt ttcttatgat ggagagcaca atgtattgaa caatatctct tttgtcgcta
     5461 atccgggaga gaccgttgca ttagtggggc acaccggaag cggaaaaagc tcgatcatca
     5521 acgtattgat gcggttttac gaattttatg aaggccagat cttgattgat gatcatgaca
     5581 ttcgagaata tccaatggaa gagttacgta agaaaatggg attggtcttg caagatgcct
     5641 tcttgtttta tggagatatc gcaggaaata tccggatgat gaatccaact atcacagacg
     5701 aacagattcg ccatgcagct gagtttgttc aagcaaacaa gttcatcgat gaattaccaa
     5761 aaggatatca ctcaaaagtg atcgaacacg gtgccagcta ttctagcgga caacgacagc
     5821 tgatcacttt tgcgcgaaca atcgtgacag atcctaaaat actagtattg gatgaagcga
     5881 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattgaca aaaatgcgac
     5941 aaggacggac aacgattgcg attgcccatc ggctgtccac gattcgggat gcagaattga
     6001 ttttagtttt agacaaaggt catatagtag aacgtgggaa tcatgaaaca cttctagcgc
     6061 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg
     6121 tgacagggct ccgttcagct ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga
     6181 tttttgcata aatttggctt atcaccgata agctggtact ggaacaccgt atattagaag
     6241 ttgggacagt agcatagtag cattttgacc taagcgctag agtgaaaata aaacagagct
     6301 tcccagattc ttcatctgtg agcgttagtg tcgtaattac cagatttaaa aaaaatcacg
     6361 acagctagtc agaagtgccc aatagccgct aagaaagcgt tgacaaaagt gcttgctttc
     6421 gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga
     6481 tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc
     6541 cctatatatc atggaaagga gtaaaacatg ctgattgaga aagtactgaa taacaacgtc
     6601 gtaataacca gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc
     6661 aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca
     6721 aatgacggac tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta
     6781 gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat
     6841 tcggtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga
     6901 atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc
     6961 atcgggaaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat
     7021 gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccaacaagac
     7081 aatacctacc tgtttactac aacaatacag gatattctaa atatcgtccg ctattactat
     7141 aagattacat ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cctacgtttt
     7201 tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg
     7261 taccaggtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac
     7321 tttttagccg agatattcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc
     7381 catattgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt
     7441 aacaagaacg catgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa
     7501 ttggagggaa tagaaatggg aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc
     7561 ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta
     7621 aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtgt tgtgacggta
     7681 atgcgagcgg gaggacagta ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa
     7741 gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga
     7801 ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct tgctattcta
     7861 gctgctggtg ggatgattcg tggactgaca gcattacttg tcttcttagg cgcatttgac
     7921 cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg
     7981 cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg
     8041 ttgcttgggg cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca
     8101 gaagcaccac tgactaccat attttctggc actatttttg aagcacctat ttatcaaaca
     8161 gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt
     8221 attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc
     8281 gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcccttagg ttttttactc
     8341 gttggaccag cattcacatt cgcaactgat attttaatgg ctggatttga aacactttta
     8401 gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat tctagtgatg
     8461 tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg
     8521 caaaatatca tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttactgca
     8581 ctctacttta agcttagaaa cccaaaagac aaagcaatcg caataccagc cattgtttct
     8641 ggtattgtgg ggattacaga gccagctatt tatgggttta cattgccacg taaaaaaata
     8701 tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtggatt aatgaattta
     8761 acttcttgga atccaggtgg attaggcatc tttacaattc ccaactatat tagaccagat
     8821 ggtgatttaa cggatgtgat taatgtattg attggaatcg ccattgcgat ggtggtatcc
     8881 tttgcgctga cttttttctt ttggaaggag gaagcaggtg aaacagatga tagacagaaa
     8941 agatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actgaatgca
     9001 gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
     9061 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacat
     9121 gcaatcggaa tggtttctga agcaggtctg gaattgctga ttcatgtcgg attggatact
     9181 gttcagttgg aagggaaata ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa
     9241 ggcgatctgt tgctccgctt cgacaaaaag aaaatcgaag aggaaggata cagtttagaa
     9301 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa tcaaggggag
     9361 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaaagaat
     9421 ggactaggct taattattct ttttagtcca cttctttatt ttcaaaattg aaaggctctt
     9481 tcatgatggc tgagctgata gggcgtttat caaatttttg tcctacagaa attagatgga
     9541 ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac
     9601 aaatcgatgt attgatcgta aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa
     9661 gtcgcaaagc ggctgtactc attgaagata gcgttgaatc accgatactg gtcaaagcta
     9721 aaattggaac atgattttgt ttcaatattt ctgcggtacg aaggatcaca ttgttttcac
     9781 ccgtataaga aatcagaatc ccgcacatat cactcgtgca attgtacgct tcatagaacg
     9841 attcaccaaa agtggtgaca atcgtgacat ccattttgat gcgcttcatt ttcagcatga
     9901 aatcttgaga aatcaaagta ttcgagttac tggcgaaaat cacgattttt tttgcttgaa
     9961 gcattagttg tttggctttt tgcagttggt catgatgaat gagagaaagt gtatcttgaa
    10021 tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt cataataccg tctgtggtgg
    10081 aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta
    10141 aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct acacggatga
    10201 gtgtggacgg gtggacataa catcgctcag caatctcttg gatggtcatt tcgccaagtg
    10261 ctggagaatc gcttaagaga aactgaatca cttcattttc tgaaggagaa aaggagattt
    10321 ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttgttttttt acgtaaatta
    10381 taactttttt gaaaatgatt t
//
LOCUS       DAZBWR010000100        10393 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000100 DAZBWR010000000
VERSION     DAZBWR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.103"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            505..1740
                     /locus_tag="ACHGT5_002239"
     CDS             505..1740
                     /locus_tag="ACHGT5_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN7218203.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2739..2999)
                     /locus_tag="ACHGT5_002240"
     CDS             complement(2739..2999)
                     /locus_tag="ACHGT5_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN7218204.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(3026..4327)
                     /locus_tag="ACHGT5_002241"
     CDS             complement(3026..4327)
                     /locus_tag="ACHGT5_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7218205.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4619..5077)
                     /locus_tag="ACHGT5_002242"
     CDS             complement(4619..5077)
                     /locus_tag="ACHGT5_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN7218206.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(5090..6421)
                     /locus_tag="ACHGT5_002243"
     CDS             complement(5090..6421)
                     /locus_tag="ACHGT5_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN7218207.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6432..7874)
                     /locus_tag="ACHGT5_002244"
     CDS             complement(6432..7874)
                     /locus_tag="ACHGT5_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7218208.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7861..8625)
                     /locus_tag="ACHGT5_002245"
     CDS             complement(7861..8625)
                     /locus_tag="ACHGT5_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN7218209.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8729..9604)
                     /locus_tag="ACHGT5_002246"
     CDS             complement(8729..9604)
                     /locus_tag="ACHGT5_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN7218210.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9859..9943)
                     /locus_tag="ACHGT5_002247"
     tRNA            complement(9859..9943)
                     /locus_tag="ACHGT5_002247"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9907..9909),aa:Leu,seq:caa)
     gene            complement(9961..10032)
                     /locus_tag="ACHGT5_002248"
     tRNA            complement(9961..10032)
                     /locus_tag="ACHGT5_002248"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9998..10000),aa:Gln,seq:ttg)
     gene            complement(10042..10114)
                     /locus_tag="ACHGT5_002249"
     tRNA            complement(10042..10114)
                     /locus_tag="ACHGT5_002249"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10079..10081),aa:His,seq:gtg)
     gene            complement(10176..10260)
                     /locus_tag="ACHGT5_002250"
     tRNA            complement(10176..10260)
                     /locus_tag="ACHGT5_002250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10224..10226),aa:Leu,seq:caa)
     gene            complement(10281..10351)
                     /locus_tag="ACHGT5_002251"
     tRNA            complement(10281..10351)
                     /locus_tag="ACHGT5_002251"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10317..10319),aa:Cys,seq:gca)
BASE COUNT         3365 a         2163 c         1601 g         3264 t
ORIGIN      
        1 acaaccaaaa atgtttagaa cagaataaaa attttaattt taaaataata ttcaaattaa
       61 acaactggcg tatctgctcc ttgatttctt tttgtgttat ctgaataatt atcgtttaca
      121 gttataacac atagtgaaaa ttgtcatggc gagaaagtct aagaatattt ttaaacataa
      181 ctatttcttt cttacgtgtg tataatgacg acagcacacc tatcaattaa tataattatt
      241 ttattttttt atgttctcat gtttattttt aatgacacaa cttctcgtat ttatctggca
      301 caacatataa atcgatcaaa aaattgttgc gtgttttttt gaaaattgga gtttcaactt
      361 gattacaaaa tttaaattta gaagttgtga aaacagcgag agacagttta atattcaaaa
      421 tatacatagg ctactcgaac gctgtgtttt ttagtatact agtaagaaac gaagggatag
      481 actcattcca ataaggagga tttcatgaag acaattcttt ttgctcctgc agtattcaat
      541 ttagctgaga caacacgaat gattgaggtt gccaaagcta tgaaagaccg ttattactgt
      601 gtattctttg gcttttctaa aacttttgct cctttgatca cagaaagtgg ctttgagtat
      661 catttactag cgccgatcct tacgaaaaaa caagaagaac aaatcataaa atttgaccag
      721 ttgcgtacac ttaaaaaccc ttttaccaaa gaaatggttg cctctcgtat cactagtgaa
      781 cagagtctga ttgctaagtt tgagcctcga ctgattgtga caggttcgaa tgtgacaatt
      841 tttttatcgg cccgcaataa aaagattcct ttagtatatg ttaaacccta tgctttgagt
      901 gaaccttttt ttcaatcaga gcatgcgctg atgccccacg agttacgttt cttcggccct
      961 ttaagcaatc ccctttggca ctggacaaaa aagcgtatag taaatacccg atggatcccc
     1021 aatgccttca aacacgctgc acaaaaagaa atgcttcctt taccaaaaac aagtttacaa
     1081 atgatggatg ctgatctaaa tctcatcaca acgcctaaac tatttttgga agaagaacag
     1141 ctgccggaaa attatcgaat cgttggtcca atttttgcta agctcagtac ttccttgcct
     1201 gaagaagtgc taacttttat acagcgaaag caacaagaaa gaaaacgtat tatctatttc
     1261 gcaatgggta gttcaggcaa tccgcgtatg ttacgcaaaa ttttgttctt cttacaaaaa
     1321 caaccggatc tagcggttat tgcacctatt gactatctgc ttgatgacat tcaatctgac
     1381 tcagaagatc ttttaataac agaatatctg tctagtcatc ttttgcatga attcattgat
     1441 ttttctttta tccacggcgg ggaagggacc gtccaaactg cctgtgaatc cggcaaaccg
     1501 tttatcggtg tggggatgca atttgagcaa agctgtaata ttgactattg tgtaaaatac
     1561 ggaaacgcaa tcgccctcac aaaaccagtg actgaaagaa aactgcagat tgctctgcgc
     1621 gaagtttctt cacccaagat tcgtcagcag gcacgcacgc tgcaagccgt ttttccaata
     1681 aatggtccac aacaagcaat tcaagaaatc gaaaccttcc tgagcaacca cccaatatag
     1741 tttcaagtag aaaaagcttt tttaatagag actcatcagt aatttcgcta ttcgagaccc
     1801 agacagtgaa aatacaaaaa aagtaaatag tggattgaaa agttagagtt acttaaatta
     1861 cggtgtctaa aaaacacaaa atcatcctta aaataaaaga tctgacgcta tccaaatgag
     1921 cataggtagc gtctgccatt tattcttagt caaattcttt gacacctttc tctggttgga
     1981 tcatctcaac gtttcttcaa aagaaagacc ataataatta ggcaaataca ttttcttaaa
     2041 atgtgtcata tagatctaga agcatcgctt tgtagagatt tgtgccgcta gtcccctcaa
     2101 caattactaa atcaaatgta ggatttacca ctttatcttt gataatcaac ctccctcttc
     2161 tgttgagaca atgatctcgc aatccgatgc atactcctat ataaactgcc agcaatcccc
     2221 acatattgag tagatcatgt cagaatgttc tatgtcgtca gcaacgatac actaaaagtc
     2281 acgttcacca tgactgattg ctattcccag cacaattatt tctatacacg tttcatgatt
     2341 tgaataaaca ctcacatcat acatattacc attcatacac catactgcag cccctactat
     2401 atggttgtaa gctcccaaat ctatatttga cttgatcact ttggtagtag atctactaat
     2461 tctaaatcca ttgctgataa aatttccatt ttttcctcct tctttctgcc aaaacagtta
     2521 aaacaataat aatctttatg taaaaaaggg ctgtgacaaa agccttgtca cagtcccata
     2581 ttccgaataa acggtggtca aaagcttgca tctgcggtaa taagttcaac caaacgaaaa
     2641 ataagccaac atttcacaaa aatttaagaa gcaattttcg taaatttctt cttatttctt
     2701 gaagctaacc acttctgtta caaccacttt tagaacattt aaagcttcga tgtgacaaac
     2761 catctatgct gctcgatcat aaaattgata gcaaaaattc cacaaagtac atatgtcccc
     2821 catgaaaaca aaacattcat atgcggtaca atgatctttg gaagaatcac gatcaaaaat
     2881 gatgccagtg cccaaatcca tcgattgatt tgaatatgat atgtagctag catattcaat
     2941 ataaggtgac tagccgttaa taaaaagata aaaacaaaaa ataaaatcag ccaatacatt
     3001 gtttcccacc ttctaaaaac caaacttaga cagtctggac ttcgttttct ccacgacttt
     3061 gttcatctga cagcatcttt ttatcataag cttttacaaa tggataatag ataataaatg
     3121 ctatcaatgt gttgatcatg atcaagataa caacacgcca gtcatttcct gctgaaagat
     3181 aaccgccgat tggagctggt aatgtccatg ttggcaaaac agtaaatcta ttgaccattt
     3241 ctaatttgac tgcaaaataa ctgataatag tcaaaactaa aggaactaag ttgaacggga
     3301 tgaacatgta aggattcaac atcataggag ctccaaaaat tatgggctca ttgatattga
     3361 agataccagg aataatagaa aaacgaccaa cctctttcaa gaattttgat ttacagaaga
     3421 atataaacaa aacagatacc acgatcgttg ctcctgcacc accaattgtt accgtccatt
     3481 gataaaactg ttcaggagcg atatagggta attgggtggc agctgtacca tctgccaatg
     3541 cctttgcatt ctcatccaac atgatcagcc acattggacg aacaacagcc cctacaatac
     3601 tcatgccatg gataccaaat gaccacaaca aatggatcaa aaacattggt agtaaaacac
     3661 caagcaaatt attccctaaa atattggtta aaggctcgaa taacgataat aaggcagcgt
     3721 ttacatcaaa gcccgcgacg cttcgaatca gccaagtaat tgttaaaatg actgcacctg
     3781 gaatcaatgc ctcaaaacta cgggcaacag caggtggcac ttgttctggc attttgatag
     3841 taatatttcg atttttaaag aaacgatata cttccactgc aaaaatcata gataagatcc
     3901 ccgcaaacat cccagatgct cccaaagaac tcataggtaa gacccagcca agaggtgcct
     3961 cttcaggagt taaaacgttt acaggaactt gtaacataaa gaacgttcct aaagataata
     4021 taccacctgt cacactatct aatccatatg atttagctag atccgaaccc ataacaaaag
     4081 ttgcataaat agccatcaat cccatcgtca tcctaaaggg taaaacaatc gttgccgaat
     4141 aaggggcaat aaattcattc catccaggaa acggtaaatt gataaagacc ataaaaaatg
     4201 tcccaatcaa aatcaatgac aatgttgaaa caaccccatt acgaattgct aataaatgac
     4261 gctgtgtacc aatttttgcc attggtggga caagtttctc ttcgacccat ttcatcagct
     4321 tttccactaa aaccactcct tttcaataaa atatgaacca ttcattgcat gacaacgcaa
     4381 agaatgttca taactaatta tataattaag aaattttatt tcaatatatt ctcttaaata
     4441 atcaaattta tttttaagaa tcctgaaaat aagttttcta aatcatagga gcaacaaaaa
     4501 aattcgataa ttaacaaaaa tcaaaatgat taaatctagt gattgaaaca tatcatttca
     4561 aaaaaatttt gtaaattcac ccataaatat caccctattt aacttttttc atcgtacact
     4621 aaaattcaaa acaactatct ttaatcttta cttcttggtt ttgttttaat aaatttcctt
     4681 caaaaatgcc tggagaaact acaatgatca ccgtatcttt ttgtgcttgc tccaacttct
     4741 tcaaatctat aatagctaac agtgtccctt cttttacctc ctggttactt tcaacccgta
     4801 tctgaaacgg acttccttcg agttcaactg tatctaaacc catatggatc agtatttttc
     4861 tgccagattc actttcgatc gttatggcat gtcgggtagg aaatatatta tcgattattc
     4921 ctttaactgg cgcatggata tatccatcat gattcgttat agcgtatcca actcctatca
     4981 tttttgaaga aaagacctcg tcttttactg ctgttaatgg aataatttga ccactagcta
     5041 cagcttttag aatgtttctt gttttaaaga aatgcacttt ttatcacctc tactttaatg
     5101 ctggccaata ttttttgttt actttgatca tttcatctag tatttttttt gcgactctgg
     5161 catttggtac ggtttgattc aatgtaaatg cttgtaaagc tttttgataa gaaccttcaa
     5221 agtaagcatc tactagtaac ttctctactg caacttgtgc ctccatcaat cctttatgaa
     5281 aatcactgat tggttttctt agtgtaattg gctccacacc agttgcacca acatatgccg
     5341 gtatttcaac aactgcatct gaccgtagat tcggaatcgc cccttgatta ggaacgatta
     5401 gcataaagcg acgacgatca tcattcaaca aggaaatagc tatatcgacg atatattgtc
     5461 catgctcccc aaaattaaag ttcaatactt cttccacaat accgcggcgt attttatcag
     5521 ccatttcttt ggtatttttc tctcgtccat ccatgaccat attagcacgt gtatactctt
     5581 tgtcagtatg ctcaacgacc atatcaggat ataagtaata ctctaagtaa ttattcggga
     5641 tattacttgg aaaatttttc gtgatgactg acatcatttg aaaggttttt tgccaagttt
     5701 tatctcctat attaaaatcg gcgacttgca tttcttttgt tattagtttt tcaataatct
     5761 ctggcataat ttctcgttgc aattctttat cataaatcga tgtataccag ccaaagtggt
     5821 ttaaaccata atacgtaacg atccaattct tacgatcata accgtaattc actgcaatcg
     5881 tttcttcaat tgaaatagtc atatcacagg cattaatcat ttttacattc ggatatttac
     5941 gacgaacagc ttcagaaacg atcgactctg gatttgtata attcaaaatc catgctttgg
     6001 gggcatagtc ttgtatatga tcgacaattt ccaataaacc tttgattgag cgcatcccat
     6061 aagcaaatcc tccgagtcca cacgtttctt gtcctactaa tccatgttta agtggaatct
     6121 tttcgtcctt ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa acaaaatcgc
     6181 atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat gcgaatattt gaaaaacctt
     6241 ttcttttcag cataaattta acaatcaagt acatatccat atttctttct tcatcaatat
     6301 catataaacg tatttcagat aatggcaacc gtttttcatt gttcaaaatg gcttgaacga
     6361 ttcctggtgt atacgtactc ccgcctccag ctatagtgat aatctgtttt ttcatcatca
     6421 tcttttcctt tctattcttc taataattta ttgaattctg ccataacata agtcacttct
     6481 aagccgatga taatttgata atttttttca gttggtttaa tgatcccgct tatccctaat
     6541 tgcttgattc gctgttcctc gaccatggtg ggatccacta cctctacccg taggcgtgta
     6601 ttacaactat agacatccag gatattggct tttcctccaa acgcttgtag catttttgtt
     6661 gccatatatt cataattttt acttgctaac cctaactctg tttcttctaa ggttacttca
     6721 ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt attgatcaaa
     6781 taataaaaag taacgtaata caaggcaaaa aagaaaattc cgattggaag aatcaaaata
     6841 gcattggtag cgattcgaaa attgatgaga taatcaacaa tacatgctcc gaatgtaaaa
     6901 ccaattcgta tattgaataa gtaacagaca atccctgcta aaccggcaaa aagtgcatgg
     6961 atcacgtaca acataggaga aagaaacatg aaactaaatt caacaggctc agtgatattt
     7021 gcaataaatg aagttcctgc accgcttccc atgattcctt tgaccatctc tttattttct
     7081 ttataagctg ctttatagat agctagacat atagctggta taccaaacat cattactaca
     7141 aaaaaaactg ataaaaacag tccagcctga ggatctccgt tgataaagcg tgtcatctct
     7201 cctctgaaaa cttccttgct aggagatgta tagcttccta aatcgtaata aatatacgta
     7261 ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat aaatatcccc
     7321 acacctaatg ctcccatatc aacgagcagt tttgcaaaat tatcaatccc aatttggatg
     7381 agcggccaga taaagcccaa aaaaacagct gcgattgttt gtagaatcat caccatagtc
     7441 aatgggaatt tttcgcccgc aaaataagaa aaaactactg gtaatttttg ttctttaaag
     7501 cgattatacg tccatgccgc tactaaaccg gaaataattc caccaaaaat ccccatattc
     7561 acttctgggt ccataatttc taagcctttt ttcaaggtag caatcgctaa aaaaccagta
     7621 agtgcaggta ttcctttgtc cttatttttg acaaaaccga ttgcaactcc tatagcaaat
     7681 aagatatcca agtttccaaa aacggcattt ccagctgcac caattactgg gatgttcagc
     7741 atatcgtcag ctgataaacg catgatcaat cccgctaatg gcatcgctgc aactggaagt
     7801 agcatcgctc ttccaagctt ctgcacattt gatttaacat tcatttttat tcctcctcta
     7861 ttaaatatcc tcatatgtta ctaactcaat caaaggatca tttttcactt tttgagcaaa
     7921 tgctgaatga attaaaaatt cgacttggtg ggtacgagga tacacaaaac ttgaaccatt
     7981 taacaattcc tgatccaaat acgctgcgtg acacatgact tcgatcgttt tatcttctct
     8041 caatatcatg agtaaacttg ccaaatattc ttccacactc gtattgattt gtcgtttttt
     8101 ttcatcagtt tctcctacga catcgaaaca aggctcaaaa taatcgacat gacgaacttc
     8161 atcttttcgt tcaaagttgc tccgaaccgg caaatcatat ttttttgcta atgtaacaac
     8221 tacctcctga ttttcatcca aagtatgagt atgatgatga ctgtctaaat gagttggtat
     8281 gatccccgca tgataaattt tttgaatctg agcattccat tcctgataca gttgatcttt
     8341 gtcaatgtta aatggatgct gataataaga taaggatctg aactctccgc tttcgataag
     8401 agtatcgaca gttttgagga gtggtttgcc acacgtcaat gttaaatgga ctcctacacc
     8461 tagcctaggc atttcctttc ttagtttcac agcatgctcg aaacccggca tatttgccat
     8521 caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat aattcacacc
     8581 atggctatag ccaaaatcat ccgaattaaa aatcactttt cccatttcat tctccttctt
     8641 ttttctacta aaataggcgc aaccaaataa tcatagtcca tttactatgt gtcatttggt
     8701 tgcgcctata gatagttacg cccttatttt aatagattct tgctagatga attgccaaat
     8761 agatgatttc attttcttga atggcaagtt gaaattgctg ttcgatgata tctttaatct
     8821 ttagtgctgt acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa ccaatatttt
     8881 ctactggcgt ttcttcctga taaatcaagc gtttaaccag gaatttgata tgaaccatta
     8941 agcgatgata agtcagtgat tctttatcat aaacgtttga ataggatttt tctagatggg
     9001 acaaaatcgt ggtcgtcatt tctactattt ttaagctctt taaactatta ggctctccta
     9061 gctcagcatt aatgatatgc atagtgatga atcctacttc atttgaccct aactcggttt
     9121 gatatcgatg attgattcgc tcgaccactt ctactgctat atcatattca tttggataaa
     9181 ggaatctcat ttctggcaac ataattgctg tgacatctct atcttctctt tttcgttgaa
     9241 tagcaaaata aatatgatct gtcaatgtaa tatagatatt tgaatctaat ggagaggaga
     9301 tcctagctgt tatcagttca atcacttctt ccgcaaaatc cataaattcg attggaatct
     9361 gcgagactat ctcactgaat cgtttgatct tttctttaga atctaataca aataatttgt
     9421 cagcatcctc aacatcaaat ggcttactca ctttagcaga aaagccaatc cctttcccca
     9481 tcgcaatttt ttctattcca tcgtcatcga tcaatacagc attatgattc agctttttca
     9541 tcacatacac cgttaattca cctcctctat atgataaaac aaaacaactt gcagactcct
     9601 tcatacatat ttttgtctcc aaacaatttt taaagacaaa aaacgaccta agaaagaacc
     9661 ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca taatttacaa aaatagttta
     9721 acacataata aaagcgttat caatatcttt tttactcgtt ttatgataat acttgtatcg
     9781 aattgatttg gaggtaatta cataaaaaca aaaaaaatat agcaactctc tataagaaag
     9841 ttgctatatt gataattata ccggcggccg gggtcgaacc ggcacgtcct caacggacac
     9901 tggattttga gtccagcgcg tctgccaatt ccgccacgcc ggcaaattta aaaataataa
     9961 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
    10021 ggctataccc caagaataaa gggcgaatga tgggaatcga acccacgaat gccggagcca
    10081 caatccggtg cgttaaccac ttcgccacat tcgccatcat aaaagatgtt atttaatttc
    10141 tcaataaaaa actaagatga agacatctta gtcaataccg gcggccgggg tcgaaccggc
    10201 acgccctcaa cgggcactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
    10261 aagttgattt attagtacta aggcggtaac cggatttgaa ccggtgatga aggttttgca
    10321 gacctctgcc ttaccacttg gctataccgc cattatacta ttatcaatct ataaaataaa
    10381 gaataaatta tag
//
LOCUS       DAZBWR010000101        10318 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000101 DAZBWR010000000
VERSION     DAZBWR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.104"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACHGT5_002252"
     CDS             complement(71..904)
                     /locus_tag="ACHGT5_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN7218211.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACHGT5_002253"
     CDS             complement(1208..3373)
                     /locus_tag="ACHGT5_002253"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN7218212.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACHGT5_002254"
     CDS             3633..5927
                     /locus_tag="ACHGT5_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN7218213.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHGT5_002255"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHGT5_002255"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN7218214.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACHGT5_002256"
     CDS             6578..7585
                     /locus_tag="ACHGT5_002256"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN7218215.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACHGT5_002257"
     CDS             7607..8626
                     /locus_tag="ACHGT5_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7218216.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACHGT5_002258"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACHGT5_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN7218217.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3198 a         2242 c         1909 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DAZBWR010000102         9988 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000102 DAZBWR010000000
VERSION     DAZBWR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.106"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(452..676)
                     /locus_tag="ACHGT5_002259"
     CDS             complement(452..676)
                     /locus_tag="ACHGT5_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218218.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            867..3644
                     /locus_tag="ACHGT5_002260"
     CDS             867..3644
                     /locus_tag="ACHGT5_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN7218219.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3725..4144
                     /locus_tag="ACHGT5_002261"
     CDS             3725..4144
                     /locus_tag="ACHGT5_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7218220.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4156..4626
                     /locus_tag="ACHGT5_002262"
     CDS             4156..4626
                     /locus_tag="ACHGT5_002262"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7218221.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4643..5422
                     /locus_tag="ACHGT5_002263"
     CDS             4643..5422
                     /locus_tag="ACHGT5_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN7218222.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5409..6230
                     /locus_tag="ACHGT5_002264"
     CDS             5409..6230
                     /locus_tag="ACHGT5_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN7218223.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6247..7296
                     /locus_tag="ACHGT5_002265"
     CDS             6247..7296
                     /locus_tag="ACHGT5_002265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN7218224.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7309..8313
                     /locus_tag="ACHGT5_002266"
     CDS             7309..8313
                     /locus_tag="ACHGT5_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN7218225.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8946..9965
                     /locus_tag="ACHGT5_002267"
     CDS             8946..9965
                     /locus_tag="ACHGT5_002267"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN7218226.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3355 a         1647 c         2090 g         2896 t
ORIGIN      
        1 ttttgtaaaa aagtgggttg agagaaatct aattgatttt caaagaagga gtatgaaaat
       61 gagaaaaata tttattcgtt tattgtgggt actatcttag aggaataaat aatagtggag
      121 aaaactatgg tttggcgcag attctatcag gtttgatgga acgaagacaa cctcccgaca
      181 tcaagtcaaa agccacttga gattttgatc tgaaaataaa aaaagaaaac aaggtaggaa
      241 catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa aactaagagt ggtgtacgta
      301 atcaataaaa aggatggtga caacagtacc tctttactct ctgaaagcat cataactcgt
      361 ttttcctcat tatgccaata aattgttgtt ataccacata taaggatttg taatagctaa
      421 aaacagacag gaaagttgaa atctgtgaat cttattttgt atggaaggat ttatagaacg
      481 caaaaatcaa gataaagatg aataaaaatc ctgtttcggc tatatattgc gtatctacgc
      541 caataaaacg agtttgttct gtcatagcca tataaagcaa aggcaaaaat acaactacag
      601 cgagataaga tccgatgatt tctcgaaaag ttttccagga agtatgtttc ctgtttttat
      661 atttatccga tttcattgaa tagtcatagc ctccttattc tttcatatgt tgaaattcca
      721 gtataaaaac tggaaggaac tgtacgtttt ttagaataaa aattataagg gataagtagg
      781 ctgctgtcta taataagata tatttttcaa ttctttatta aagattttat tgacaaaatg
      841 aatccgtttt ctatgatgga ggaggggtga ataagttgtt gaagaaggaa attttatgtt
      901 acttagagaa ccagacagcc tttttcgatt tagaacatat gaacgaggtc tttacagcac
      961 aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt cagtcattat ttgaaccagt
     1021 taaatgatga gcaactacta gtcaaaatca attctcgacc agtgatttat ttccacaaag
     1081 aagcttttga aaaacaattt tttaagttac gcacgaattt ttatttttct atcgatgatc
     1141 tcaaagagga acagcctttt ttcgcgcaac caaaagactt attctctttt atgatcggat
     1201 acgacgccag tttgaaagaa agtatcgaac aaatcaaaac agctctttac tatccagatg
     1261 gcggattacc acttttgatt acaggcgaaa gtggcacagg taaaagttat ttagttaatt
     1321 tggtctatca gtattgtctg cttcatgatc tgctagaaga ttctgcacca ttcatcactt
     1381 ttaattgtgc acaatatgca gataatcctg aacttttgac tagcaatttg tttggacatg
     1441 ttaagggagc gtacactggt gcagaagaaa ataaaaaagg ggcttttgaa gcagcagatg
     1501 gtgggatctt gtttttagac gaggtacatc gcttaacacc agaaggtcag gaaaaactgt
     1561 tcacctatct tgatcaaggt gtgatttatc gtatgggaga acctaatttg tcacgccgga
     1621 tcaaaacacg tctctttttt gctacgactg aagagattac tagtcatttc ttaacaactt
     1681 tcattcgaag aatacctatc cgaatcgaac tacctgcact taatcagcgt agtcgtaatg
     1741 aacggctaga actgatttac tctttttttc tcgaagaaca aagaaaaatg gggagccctt
     1801 tgtcagtcag tggaccagct ttgtcacttt taacaagtca gaaattatcc ggaaacatag
     1861 gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc atatgctgaa cagcgtgaac
     1921 agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga actacttgcg cagcctatta
     1981 ctaaaaatac agcacagcaa ggaatctata ttacgcaaga ccagacctta gagcaattaa
     2041 tggagaagca acagcccgaa cagcagcgaa tcgttaaaag ttttgagcaa atcctaattg
     2101 atttcaagaa aaataactgt ctgctatatt cttgcgaagg aaaattaaag caagaagtga
     2161 ttcaattatt tgattttttg ctgtttgaga ccagtagaca agaaaaacac gagctgctgg
     2221 tttattggac acaatctatc cgagagacct ttcgacaaat ggaaactgcc tatcaaatca
     2281 aattcgatgg aaacagtgtc tatgccttga gttactatct ctatcagcga agcactatca
     2341 aatggttacc ggaagaacat gatattatcc aactaatcaa agaattagaa caacaagttt
     2401 ctcagtctta tccatctagt tatcactatg tccaacgaat tctggaatta agtaagatca
     2461 aattagatat tgaagacaca agtatggatc ggattttact gacgatctat ctaaaaaaag
     2521 cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat tgctgctcac ggttatgcaa
     2581 ctgctagcag tatggcaaat gtagtaaatc gtttattaca agaggatcta tttgaatcat
     2641 ttgatatgcc actagatgtc acaccacagc agattgcttc agaaatcctt gattattgcg
     2701 aaaacagcga tgtttcaaat ggattgatca tattagtaga catgggatct ctgaaagaaa
     2761 tccatcagtt ttttaaaaaa cagttatccg taccgctttt gattttgaat aatgtgacga
     2821 cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa tgtatcatta gaagagatag
     2881 cagaagagac tgttcgacag atccagccag aatggcgact gctctatcca gaggaaaaca
     2941 aacccaaagc actcattact acttgcttca ctggaatcgg tacggcggtc cacttaagtg
     3001 aactgctaga aaaaagctta cctacggctt gtcagttgaa aatcatccca tatgaatatc
     3061 agcaattaaa agataagaaa aacagtgacc cattattttc catatacgaa gtcgtaggaa
     3121 tgattggcac cacagatcct gatatcacca atatcgacta tctttcatta gaagacctca
     3181 tctcggggga aaagatgtct gtactagctg aatggttgga cactactatg aatgcttcag
     3241 agaaggaaat attcaatcag cgcatcattc gtaatttttc attggaaaaa gtactggatt
     3301 ccgtgactat tctagataca gagaaggtga tgggagaaat cgatcatttt atgagagaat
     3361 tagaggtgca gttgaatcga agcatcagca atcccaaaaa attagcgttg tatgtccatg
     3421 tgagctgtct cattgaacga ttgatccgac aaatgccaat cgaaacctat caaggattag
     3481 agaaatggaa gcagtgtcaa aaagaagggt tatctgctat taaatctgcg tttagtgtca
     3541 ttgaagagca ttatagtgtc atgatcccca attctgagat tgcctatatc tatgatattt
     3601 tgagtgaaga tacagagttt ctctcgatag aagaagaatt ttgaacataa gcaagttgtt
     3661 gccactaatt ttggcatgca acttgctttt ttagtatgtg taagtgaaaa gaggagatga
     3721 aaaagtggat agagaaatta tcttagcttc ccatggaaaa tttgcttcag ggatattaga
     3781 ttcattagag ctgatctatg ggaaaaatca ttctattact gtattcgatt gctatactga
     3841 tgagaatttt gatttagcgg aaacagtaag gcagcttttt atgaggtaca aagataaaga
     3901 aatcattgtt ctgacggatc tttttggcgg aagtgtcaac aacgaattct tacaatatat
     3961 caatcgggat catgtctatc tcgttgccgg attgaatttg ccactattga ttgagctagt
     4021 atctcggttg gacgctgaag tagagacaac tgttttgatc cagcaggtgc taactgattc
     4081 aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag aaaatagaag aagacgaatt
     4141 ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc atcggttatt acacggacaa
     4201 gtagcttttt catggacaca aacaataggc gcagactgta ttttgattgc taatgatgat
     4261 gtacctacga atgagatacg aaagacaacg atcaaattag ccaagccaca aggagtcaaa
     4321 ctagtgatca aatcaattga tgactcgatt gccgcgttga aaagcggggt gacagataaa
     4381 tataagttat tcatcgtagt ggaatcgatc gaggatgcat ataagctggc agaagcgtat
     4441 ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa aacctggtac tcgcagcatc
     4501 ggaaaagcgg taaatatttt accagaagag gaggaacgct tgaaacgatt aattgacaaa
     4561 caaatagaag tagaaatccg tcaagtacca gcagataaaa aagtggatgt aagagaagtt
     4621 ttgtaagaaa ggaagaacct caatgattgt acaagcagtc ttattaggaa ttgttgcatt
     4681 tgtcgcacaa tcagaatatg cactagggac atcgttgctt tctcgtccga ttgtgacggg
     4741 gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg atcattatgg gcgcaacttt
     4801 agagttagca tttatcggat cgttctcagt aggtgcttcg attccacctg atgttgtgac
     4861 cggtgggatt ttagggacgg cttttgctat tacagctgga gccggtacag aaaccgcgct
     4921 tttactaggt ttgccgattg ctacgttgac attgatattg aaaaatattt acttagggct
     4981 tttgattcca attatgaacc ataaagcaga caattatgca gaagaaggaa attataaagg
     5041 agtcgagcat atgcacttgc ttgccggtat cggtctttcc ttgatgcttg gtgttgtggt
     5101 aatggtttct tatatggtgg gaagcaatac gatcggaaat ctattaaata tgattcctga
     5161 cttcgttcaa aaggggttgt cagtagctac agggttgatt ccagctcttg gttttgcgat
     5221 gcttgctcgt ttgctgatca acaaacaagt tgcaccatat tttttcttag gttttgctat
     5281 agcttcttac ttagagattc ctgtgaccgg agtagctatt tttggtgcga ttttagcagt
     5341 tgtggtagtc aatattatga atgtccgtca actaccactt caaacaactg caggggaggt
     5401 agaagaagat gaagaattct gagatcaaaa acagcaaaat aacgaaaaaa gagctaaatc
     5461 acgtattttg gcgttctttt caaatggagt tttcttggaa ttatgaacgt cagatgaata
     5521 tggcttatgt ttatgcaatg attccgattt tgaaaaagtt atatcagtcg aaagaagaaa
     5581 tggcagcagc tttgaaacgg catctagagt tttttaacac gaccccacat atcgttacac
     5641 tgattttagg aatcacggct gcaatggagg aagaaaataa ggatgatcct gaatttgatg
     5701 tgactgcgat cgatagtatc aaaacatctt tgatgggacc tcttgcaggg ttaggagatt
     5761 ctttcttttg gggaactttg cgattgattg ccacaggtgt gggtacctct ttagccatgc
     5821 aaggaaatat tttaggccct atcttgttca ttttgatttt caatattccg cacattctgt
     5881 ttcgctatct agcaacaggt tggggatatc gcttaggaac gggttttttg aaaaagatcc
     5941 aggcaaacgg tatgatggaa agtttgacat taggcgcgtc tattattgga ctgatggtcg
     6001 ttggagcaat gactgccacg atgatcacca tcaatattcc tttaaaaatc ggttcaggag
     6061 aaaatgctgt gactgttcaa agtatttttg atgatatcgt tccaaatatc ttatgtcttg
     6121 gagcatttgg catagtgttt tatctattga agaaagaagt gaaaccatta acgatcttga
     6181 tcggactagc tttctttgga atttttggtt cgtggatagg gattttttaa aaaaggagca
     6241 gagaaaatgt ttacgatgca agattatatc tatgaagaaa aggaagtact aagtacgatc
     6301 ttgaaaaaga atgatttttc gacaagagaa catatgaaaa aaacagtgaa tttactgatc
     6361 ttagcaacgg gttcatctta taacgcttgt ttagcagcga agccagcatt ggaatcttat
     6421 ggtgatctta ctgtcgatat tcaagaacct ttttattttt tcaattatgg aaaactttct
     6481 ccttcaatcg atactgtgat tgctgtgtca caaagcggga aaagtgcttc tactgttgaa
     6541 gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg ctatcacaaa tgacgtgcaa
     6601 agtccacttg ctttagaagc tgcacaaatt attgatttag gtgccggggt agaatcagta
     6661 ggcttcgtta caaaaggcta ttctgcgact gttttacagt tactactctt agggatagga
     6721 attggaataa gtaaaaataa aatcagcaaa aaaatagaac aagactacat gcaacagttg
     6781 cgcaaaataa tcaaccattt accagcgatt attcaaaaaa cggaagaatt ttttgatgga
     6841 tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca ttggatatgg tccaaactgg
     6901 ggaacagcta aagaagcaga gacgaaattg actgaaacaa tccgcgttcc gtcacaagga
     6961 tttgagttag aagcttatat gcacggccca tacctggagg cagatgcttc gcatttgcta
     7021 ttctttattg aaggggaaag tgtaaataag gaacgttcgc aaaagttgca aagatatatg
     7081 agtcggtacg ttggagagac tctcaccatt accacaaaaa aagcaagaaa cgagaaaaca
     7141 cttggattag caatagaatg tgatgaatat ctgtctgtat tagcgcttgt cgttccgttt
     7201 caactattcg cttataaaac cgcagtagct aaaggtattg acttgaacaa aaagattttt
     7261 gaagactttg atacagtctt gaaaagtaaa ttataagagg tggaaatcat gctgaaattc
     7321 aatgaagaag aacaaatcaa agcaataaaa ggcgcactag ctttacgtcc gcaagtagaa
     7381 gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg tctattatct aggaatcggt
     7441 ggaacgtatg cttcctcgat gcaagctgtc acttatatga atgggaaaag caacttgcct
     7501 gtctttgtac agcatgctgc agaatattac actactggaa acaaaagatt aacaaaagat
     7561 tctatcgtta ttttgtcctc tgttaccggt acaactcaag aagtagtgaa agcagtagaa
     7621 caaatcaaga aagtaggagc gacactgatt ggatttatcg ataaagcgaa cagtaagttg
     7681 tctcagctat gcgattttgt ggtcacttat ccagctcctg gaacagaaca aatcaaattt
     7741 tttatggcag cagatcgatt gatgtatttg aatggagaat tcgaagctta ttcagaatac
     7801 tatgaacagc tcgaacgtta tttaccaaca gggttagtgg aagcagagaa aaaagcagat
     7861 gcatttggct tatcatttgc agaaaaacat cgacgtgata gtatgcatta ttttattgga
     7921 gcagggaatc aatggggagc tgtttattca tatgccatgt gttactggga agaacaaagc
     7981 tggcttccat cgaaatctat ccatgctgcc gagtttcttc acgggacgtt agagatcgta
     8041 gaagaaacaa ccccagtcac attattttta ggcgaagatg aacagagagt gttatctgaa
     8101 cgtgtggcta aacttttgcc aaaaatttgc agcaattata cattgataga cacaaaagac
     8161 tatccagtag aaggtatcag tgaaaaatat cgcggacgtg ttttgtcttt cttattgatg
     8221 catgtagtga cacaacgcat cgatgctcat gtggagcagt tgaactgcca tccattagag
     8281 atacgccgct actatcgtca gtttgattat taggatagac tggataaaaa gaatataaaa
     8341 aataaagaga gcagcgataa ctgctccctt tattttttta aaatattggc atcaattacg
     8401 ttataaaaca cattttttgc attatctaca gttcaactta taatctgttt aatgggacat
     8461 gtgggtgcca accatttttt gttatgcaat agtactattt agttgttgca tggatccgta
     8521 gtaaaagtcc aagtacaacg agttgcgcca gtttacatag cgttttgtat ccagaatgtt
     8581 gactaaaaca aaagttccta tgtcaaccgt caacagagac aatctgtccg ttgcaggacc
     8641 tgacagattt agaaatgttc aatctgtctc ttatgtattt taaatcaata ctcactagga
     8701 gaagtttcct aacctcctaa tagtaatgct ggtagtaaca ttgtcaaaac tgcttcctct
     8761 taggaacaag caaagcataa cgagtgtaaa aataaaatgt agactattgt aacatatcat
     8821 tgattatttc aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac aatggaaatt
     8881 agtcccaact ttaagaaatg tatactataa tggaaattat caattattta tttggaggaa
     8941 tatccatgaa acaaaaaatc gcagttcttg gtcctggatc ttggggcact gctttagcac
     9001 aaacattggc agaaaacggg catgatgtcc gtatttgggg aaatgtacct gaacagattg
     9061 atgaaatcaa taagtatcat acaaatcagc attttttacc tgatttgacg atcccagaat
     9121 cgattatcgg atataaagaa ttggcagaag cagtggataa cgcagatgct attttatttg
     9181 ttgttccaac aaaagcgatt cgttcagtcg cacaggaact tattggtaaa atgaatacta
     9241 agccaatcat tatacatgca agtaaaggac tagaacaaga tacgcataaa cgaatctcag
     9301 aagttttggc tgaagaaatt ccagaagaaa aaagacaggc aattgttgta ttatctggtc
     9361 ctagccatgc tgaggaagtg gcagtacatg atatcacaac aattacagct gcaagtattg
     9421 atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa tgattatttc aggatttata
     9481 caaataatga tgtgattggc gtagaaactg gtgcagcact gaaaaatatc attgcaattg
     9541 gtgccggcgc tatccatgga ttaggattcg gagatgatgc aaaagcagca atcatgactc
     9601 gaggattggc agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct ctgacattta
     9661 tcggtttgag tggtgtgggg gacttgatcg ttacttgtac aagtgttcat tctcgcaatt
     9721 ggcgtgccgg taacttgtta ggacagggac ataagctgga agaagttcta gaaaacatgg
     9781 gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt agagctagct caacagctag
     9841 gtgttgagat gccaattact caaacaatct ataatgtttt gtataatgga gaagatatca
     9901 aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa ttttctcttc
     9961 gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DAZBWR010000103         9875 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000103 DAZBWR010000000
VERSION     DAZBWR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.107"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..268)
                     /locus_tag="ACHGT5_002268"
     CDS             complement(23..268)
                     /locus_tag="ACHGT5_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN7218227.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(383..961)
                     /locus_tag="ACHGT5_002269"
     CDS             complement(383..961)
                     /locus_tag="ACHGT5_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN7218228.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1038..1568)
                     /locus_tag="ACHGT5_002270"
     CDS             complement(1038..1568)
                     /locus_tag="ACHGT5_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN7218229.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1572..2432)
                     /gene="rlmB"
                     /locus_tag="ACHGT5_002271"
     CDS             complement(1572..2432)
                     /gene="rlmB"
                     /locus_tag="ACHGT5_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN7218230.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2425..2820)
                     /locus_tag="ACHGT5_002272"
     CDS             complement(2425..2820)
                     /locus_tag="ACHGT5_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN7218231.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2817..4229)
                     /gene="cysS"
                     /locus_tag="ACHGT5_002273"
     CDS             complement(2817..4229)
                     /gene="cysS"
                     /locus_tag="ACHGT5_002273"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN7218232.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4226..4762)
                     /gene="epsC"
                     /locus_tag="ACHGT5_002274"
     CDS             complement(4226..4762)
                     /gene="epsC"
                     /locus_tag="ACHGT5_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN7218233.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4848..5087)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5084..6544)
                     /gene="gltX"
                     /locus_tag="ACHGT5_002275"
     CDS             complement(5084..6544)
                     /gene="gltX"
                     /locus_tag="ACHGT5_002275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN7218234.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6714..7871)
                     /locus_tag="ACHGT5_002276"
     CDS             complement(6714..7871)
                     /locus_tag="ACHGT5_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN7218235.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7905..9275)
                     /gene="radA"
                     /locus_tag="ACHGT5_002277"
     CDS             complement(7905..9275)
                     /gene="radA"
                     /locus_tag="ACHGT5_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN7218236.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9338..9865)
                     /locus_tag="ACHGT5_002278"
     CDS             complement(9338..9865)
                     /locus_tag="ACHGT5_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN7218237.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2509 a         2214 c         1785 g         3367 t
ORIGIN      
        1 gaatctcagg ctcgtttgct ttttaaactg cttcagttaa aaattcgatc tcaactgtcc
       61 gagtaagtac atctgaatag ctgtaagata ctcgttcaaa agaattttct tcaggatcca
      121 aatcaacaac gaatactgaa ggataggttt ctgttaaaac gccgcggcgt tctgtttgac
      181 gctttcttcc tgtctgagca accaacatga ttgggctgcc gatacgacct tctaaatctt
      241 tcttgattga agctaaagtt gttggcatac agttcacctc tactatcaac tataccacat
      301 atttttaaaa ttttcaagtt taccataaaa aaaaaagaaa tgcaataaaa ataaataaga
      361 agctcattaa gatttttcta aattattcct cattataaaa tttcaagtgt ttttttattt
      421 tgagttttac tctcccgtaa gcgctcatta cttgcttgtg atccattgct aaacattcag
      481 ccatgtctac aaaatcaacg ccttgcatat aataatgaaa aattttagct tctatttttg
      541 aaaactggat ttcgcaattt tcgattgctt cttttaataa aagatgctga atcgctaaag
      601 gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc tgtctctatt ttttggtcga
      661 ttgagacttc atctttttga tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat
      721 agatttgatt gcttataatg cttttataat aacttccaaa gctaacacct ttgctttttt
      781 cgtaagttct caatgcctga tataaagcga tccttgcttc ttgttcccaa tcctcagaaa
      841 cataatttct taaataatag gtgtttttca aacgtagtaa aagtggaaaa tatttttgat
      901 acagggcagc aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca
      961 taaaataatt cctccataaa ttcgtttatg gaggaatctt atcatcaatg attcatcgaa
     1021 ctgattagaa cgatatgtta tttatttttt ctcattaaat cgtccaattt ttgggacaat
     1081 ttttttaact gctcttcatt ccatggagaa ttacgtcgga aatcaataaa atgcatatct
     1141 ttattttctt gggctattgt tttttccgtt ttttttatcg tcttatataa ttcataagca
     1201 gaagttcgta atgctccttg ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc
     1261 actgtcactt gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat ataactgtca
     1321 gccgtttcgt cttctttcgt aaaaacaaca tccaactggt atttcttata attttgttgg
     1381 atgcccggaa ctagttgggc atcgaagaca acgatgactt ccattccttc atatttcgca
     1441 taatttgata aacgatgtaa aagagcttct cttgcatccg ccattttgtc ctgcttttta
     1501 agctggacaa gttccggcca cgaaccaatc atgttgtagc cgtctacaat caacagctgt
     1561 tttttcatgg tctactcccc ctgacgttgg gaaaaagcct gatacataag caaaccagca
     1621 gcaacactgg catttaagct ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc
     1681 atttcttttt tcaatcctgg actcattcct ttgccttcat taccaatgac taaagcaatt
     1741 gatcctttgg cattccattt ttggaagttt gtgccggtca tatctgtgcc aaatacccaa
     1801 aatcctgttt ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg
     1861 acatgttcga ctgccccagt ggaagtcttt gttacgatag gtgtgattcc aactgctcgg
     1921 tgttttggta tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca
     1981 aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga aggggtcttc ttttttctct
     2041 tttgttcgtt caatcagttg gtcaagggat aaatattcat aaggtgtaat cgcaagaacg
     2101 actccttgat gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg aacccatttg
     2161 actggcacag cttgttcacg tgcgatttct ttcagttctt ctactttttc tcctcgtgaa
     2221 tcttcttgaa gaaagagttt attccctcgc ccttgttgta gcgcttctac tgcggcatgg
     2281 tgcccaaaga cgaaattatc ttctaatgct tcttcatttt gatcagaaac tgactgattt
     2341 cgtccagttt gctttttatt tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc
     2401 gattgatgtt tgtttgttct ttctttaccc atttttttca ccaactttct gaatacacca
     2461 gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc
     2521 ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc tcgtatggct
     2581 tttagcattt cttcctctgc gataataaag ttcttcttct tctgtcaaaa gatgttcttc
     2641 cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa acataattcg ttgccgcacg
     2701 atgcagctta ttgggttttg tttctccttg atcaatcagg tggtcacgaa catagatttc
     2761 gtaaatcgca tctccaacat aagccaaggc taaaccgttc aattgtgtgt aatctctcat
     2821 gcttctcttc tccatcgggt tccttgcggt gtatcttcta ataagatccc tttttctttc
     2881 aacaaatcac gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt
     2941 tcttcgatca attgttcaat ctcttcatct aataactctt ctgctgtaaa taaaataccg
     3001 aagatcgaaa gccattgcga gaataaagct tctccttgtg ccaagacagc ggcagaaact
     3061 ttcggctggt ctagataagt gttcagccac ttagcaagct catagaccac tgtgatccca
     3121 tttgctgcat taaaatcatc gtccatttct tcgatgaatc gctgttctaa attttctaac
     3181 aaagcgattt tatccgcatc atctgccaaa ttctctgagg cagtttcttg gcggaatttc
     3241 aaattgtcaa acgtattctt cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt
     3301 tcactgtagc ggatcggacg acggtattgt gtggtcgcca tgaagaaacg caagacttgt
     3361 ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat tccctagaga tttactcatt
     3421 ttttcatcgt cttcgccaat cgttacatag ccattgtgca tccagtaatt agcgaatttt
     3481 ttccctgttt tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct
     3541 tgcccgccac catggatatc gatcgtttct cctaaatgtt ttgttgccat aacggaacat
     3601 tcaatatgcc aaccgggacg tcctttgccc catggtgaat cccaagaaat ctcgccaggt
     3661 tttgcttgtt tccataaagc gaaatctaat gggtcttctt tttgctgctg ttcttcccct
     3721 gtacgctgac ttgctccgac ttccagttca tcgatggatt ggtcacttaa tttcccataa
     3781 ttttcaaatt tacgggtacg gtaatacaca tcacctgaag attcataagc atagcctttt
     3841 tcaaccaaca cttgaataaa tgctaaaata tctggcatat gatccatgac tcttggatgc
     3901 agtgtagcag gctcgacatt caacgctttc gtatcttctt caaatgctcg gatgaaacga
     3961 tcagcaactt ctggtgctgt aatacctaat tcgtttgctg tacggatgat tttatcgtct
     4021 acatccgtga aattcgatac gaaatcgact ttatagcctc gatattcaaa atagcgtcga
     4081 attgtatcga aagcaatggt gctacgagca tttccgatat ggatataatt atacacagtc
     4141 ggtccgcaga catacatacc gacatgtccc ttttgtatag ggatgaattc ttcttttttt
     4201 ctggtcaatg tattgtagat ttttatcatt ctgatctcct cactttttct cctttgattc
     4261 gaacgacttt tgctggtact cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa
     4321 cggcacctgc accgattttg gcgttttttc cgatcgttac cggtcctaat atctgcgcat
     4381 tcgcagagag catggctcct tgtttaacag ttgggtgtct tttacccgtt tcttttcctg
     4441 tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc
     4501 caatgacgat tcccattcca tggtcgatga acacgccagt tccaatcgtt gctcctggat
     4561 ggatctcaac accagttagg aaacgccaga attgagcatt gatcttcgcc aataaaaaga
     4621 gccggtgctg atataaaaaa tgagagaaac gatgccaaaa caaggcatga aaaccagggt
     4681 atgtcaaaac gatctcagct gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa
     4741 tcgctcgggt aaaccaactc atgactttcc actcctttcg taagaatttt atgtaggaaa
     4801 tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt ttccttatca aaagacgctt
     4861 tccgcgtggt tccacttttc ttcataatca atgattatct ctgacagcta acgcctgtta
     4921 tacgttcttg gctactttgt ttcctataaa acagtttttc ctgttttctg tgtttcgcca
     4981 agaccactcc cagaggcatt tcggcaacca ttcattgata ccttgcacca cccggtatct
     5041 ctcttgtaat gatcagctgc ttactttttt ctgttcatcg tgtttagttc agtacttgat
     5101 ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat cgtttcaggc aattctggac
     5161 catgcatttg tcctgataca gctacacgga ttggcatgaa aagatttttt ccttttacgc
     5221 cagtttcttt ttgaacagct ttgatggcag ctttgacagt tggagcatct actacttcca
     5281 tttgttctag ctgtgctttg aatgcgtgga gaacagtagg aactgtttca ccagcaagta
     5341 cttcttttgc tgcttcattc aatacaggat gttcattgaa aaatagcgta gacagatcaa
     5401 cgatttctgc agcatagctc atttgaggtt gatacaggct aacgatcttc ttcacccatt
     5461 cgattgtctt ttcatctggg ttttctgcta cacgtccatc agcgattaga taaggcagac
     5521 acatgtcagt aaatgcatct agatccattg ctttgatgta atggttgttt acccattgca
     5581 gttttttcgc atcgaaagct gcgggtgatt tacttaggcg ttttggatcg aacattttga
     5641 tcaattcttg gtgttcgaag atttcgtctt cgcccacagg tgaccagccc aataatgcaa
     5701 taaagttgaa cattgcatct ggtagatagc ctaattcgcg atattgttcg ataaattgca
     5761 agattgattc gtcacgtttg cttagttttt tacctgtttc actgttgatg atcaatgtca
     5821 tatgtccgaa ggttggtggt gtccagccaa atgcttcata gatcatcagt tgttttggtg
     5881 tattggcaat atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga
     5941 ccgcaacagc aaaattataa gttggcatac catcgcgttt ttgtatgaca aagtcgccac
     6001 cgatattatc ggattcgaac gaaatagcgc ctttcaccat atctgtaaat gcgtattctg
     6061 tattgcgagg cacacggaaa cgtacaacag attctagacc ttgtgcttct ttttctgctt
     6121 gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc
     6181 gttgtgcttc ccgttctgct tctaattctt cttctgtgca atagcatttg tatgctcgat
     6241 tgctagcaag cagctgatca atcagcggtt gatagatttc tttacgttca gattgacggt
     6301 aagggccgta ttctccagga ttttcaggag attcatccca attcattccc aaccaagcaa
     6361 ggttttccaa ctggcttttt tcgccatctt cgatattacg tttttggtca gtatcctcaa
     6421 tacgaataat aaagtctcca tcattgtggc gggcaaacaa ataattgaat aatgcagttc
     6481 ttgcatttcc aatatgcagg tgcccagttg gacttggtgc atagcgtacg cgtactttcg
     6541 tcattttctt cctcttcttt caaactagac tttgactaac agcaaaaatt ccgtcagtta
     6601 cggcttctca aatatcattt atttaaagcc actcagcaca aattttacct tttctaaggt
     6661 aaatagtaaa gccaactggt tgaatttcct taccagttgg ctgaatttct tttttatttt
     6721 gattgtttag tctttttctc tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca
     6781 ggtttagcaa agatcatgcg tccagcagct gtttgaaggg cacttgttac aacgacttcg
     6841 atacgttcat tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg
     6901 taggctactc cttgttggcg ttctgtcccg gcttttacta ctagaacatt catcgtttca
     6961 ccagggatga cgactggttt tacggcattt gccaacgcat tgatattaag aacaggtaca
     7021 ttttggaatt cagagacttt attcaagtta tagtcattgg ttacaatgac accatctaaa
     7081 agcttagcca atttgatcaa tttgctgtct acttcactga tatcttcaaa gtctccatcg
     7141 tacatttcga cagatatacc ctcttctttt tgcaaggcat ttaggatatc taatccgcga
     7201 cgaccacgca cacgtttcaa actatcccct gagtccgcga tgtattgtag ttcgtaaaga
     7261 acaaaattag ggatcatcaa ggttccttct aagaatccag tctttgtaat atcataaatc
     7321 cgaccgtcaa tgatcacgct tgtatccaag attttatatt tatggaaatt ttctcctgat
     7381 tttcgttcta acagttgact ctcaacttga ctagctgttt cttgctcgat ttttttgctt
     7441 cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg ttcccattct caatcccaga
     7501 tagcctaaga tgatcatcag taaaatcggc aatacactat tgacgaatgg aatttccata
     7561 ttgtaaagag gggtcgagat cacaacacct aaagtcaatc cgatgatcgc accgatcgca
     7621 ccgaagagaa gataagtcaa gctgacctca cttagttttt gttctacttg tttgataata
     7681 gctgagatat gtttggccaa cagcaaagat aataaaaaga aaataagtgc gcctaacagg
     7741 ctgttggtaa atgtattatt cagccatccg tttgactgtt gaccgattgc ctgccaagct
     7801 gagggtaaaa atgaaatacc caagcttgcg ccgacaatga tcatcagcaa agtaatgaca
     7861 cgtttttgca tcgtttttcc tccgtttcga caatcattta ttagttagcg gaacacacgc
     7921 ttcaacgttt cagccaaagt tgctactcca actatctcga ttccttttgg cgcttcccag
     7981 ttacctaagt tgtttttagg taggtatact tttgtgaagc cgagtttttg gacttctttg
     8041 actcgttgtt caatatgact gacacgtctg atttctccag tcagtccaat ttcaccgatg
     8101 aaacattccg ttggctgtgt tcctttttct ttatagcttg aagcaatact gacagcgata
     8161 gctaaatcaa tcgctggttc atttaatttt accccacctg cagcttttaa gtaagcatct
     8221 tgattctgaa gaagcaaccc agctcttttt tccagcactg ccatgatcag agatacccga
     8281 ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga acatagtagg tgtgactaat
     8341 gcttggatct ctaccaaaat tggacgtgtc ccttccattg ccacaacgat agcagagcca
     8401 gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag gattagcgac ttcttccaat
     8461 ccatgctcat gcatttcaaa aatcccaatc tcattcgttg agccaaatcg gttttttact
     8521 gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa aatataacac cgtatccacc
     8581 atatgttcta acattcttgg gccggcgatc gagccttctt tggtaacatg accgacgata
     8641 aaaatggcta tcccatttgt tttagcgatt ttgagtagtt ctgcagtggt ttctcttact
     8701 tggctgacac ttcctgccac acttgtgatg tctggctgcg tcatcgtttg gatggaatca
     8761 atgataacat aatcagggga gatatgttcg atggcacgac tgatttcgtt catatctgtt
     8821 tcagcgtata gataaaattc tgtgttgata cttccgaggc gttccgcgcg taattttatc
     8881 tgctctgcgc tttcttcgcc ggatacatat aagacttttc cgcctatggc tgctaattgc
     8941 tgagaaactt gtaacagcaa agtggatttc ccgatacctg gatcgccacc aatcaagacc
     9001 atagaccctg gcaccacacc tccgcctaac acacggttca gttcttctaa ttttgttttg
     9061 atccgaggtt cttttttagg gacgacgtct gcgatttttg ttggttttgc tttttctcct
     9121 gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta
     9181 ttccattttc cgcaatttgg gcagcgtccc aagaacttag gcgacacata cccgcaattt
     9241 tggcaaacaa attgtacttt tgattttttt gccattgtac cgcctccttt tatcctaatc
     9301 atgagacatt aaatcaagct taagtatctt taacgtttta tttactggaa gaaccaaatc
     9361 cgccttgacg ttgagcagtc gtttgatctt gatctgcttt taaaaacggc aaaaagatcc
     9421 cttggccgat acgctcgcct tttttgatca ctacatcctt gaaaccaaag ttggtgaatt
     9481 ggaacatgat atggccttca tttgcctcat tattgtaata atcactgtca atcacaccta
     9541 ctccgtttgt caacactaaa aaccgcttca acggattgct tgaacggtta gcaagctgca
     9601 aaaattcatc ttctcccatg taagctttga ttccagtagg cacgagggtt ggtttcaatt
     9661 gtttttcatt ttctgataca tcttttttca atccatctgc gactgtttcc aacccctttt
     9721 tccagcctgc tttccagata cttggaatca cgacctcttc tgctgcttca aaatcatagc
     9781 cagcagcatg atgtgtagcg cgctgcggta gattgatatt ttgatcttta tatttagaaa
     9841 taacttcaaa tcctcttttt ctcaccggtt ttcct
//
LOCUS       DAZBWR010000104         9796 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000104 DAZBWR010000000
VERSION     DAZBWR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.108"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1031)
                     /locus_tag="ACHGT5_002279"
     CDS             complement(183..1031)
                     /locus_tag="ACHGT5_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN7218238.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1195..2097
                     /gene="gnd"
                     /locus_tag="ACHGT5_002280"
     CDS             1195..2097
                     /gene="gnd"
                     /locus_tag="ACHGT5_002280"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN7218239.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="ACHGT5_002281"
     CDS             2099..3100
                     /locus_tag="ACHGT5_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN7218240.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="ACHGT5_002282"
     CDS             3097..3381
                     /locus_tag="ACHGT5_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN7218241.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="ACHGT5_002283"
     CDS             3414..4211
                     /locus_tag="ACHGT5_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN7218242.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="ACHGT5_002284"
     CDS             4225..5058
                     /locus_tag="ACHGT5_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN7218243.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="ACHGT5_002285"
     CDS             5076..5933
                     /locus_tag="ACHGT5_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN7218244.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="ACHGT5_002286"
     CDS             5955..6464
                     /locus_tag="ACHGT5_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN7218245.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="ACHGT5_002287"
     CDS             6501..6902
                     /locus_tag="ACHGT5_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218246.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="ACHGT5_002288"
     CDS             7014..7367
                     /locus_tag="ACHGT5_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218247.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="ACHGT5_002289"
     CDS             7401..7844
                     /locus_tag="ACHGT5_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7218248.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="ACHGT5_002290"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="ACHGT5_002290"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN7218249.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="ACHGT5_002291"
     CDS             8939..9652
                     /locus_tag="ACHGT5_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7218250.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3255 a         1552 c         2165 g         2824 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt aatgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgtgt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gtcatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgtt
//
LOCUS       DAZBWR010000105         9703 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000105 DAZBWR010000000
VERSION     DAZBWR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.109"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..265
                     /locus_tag="ACHGT5_002292"
     CDS             <1..265
                     /locus_tag="ACHGT5_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN7218251.1"
                     /translation="DQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQDFIVAIQG
                     VNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            295..417
                     /locus_tag="ACHGT5_002293"
     CDS             295..417
                     /locus_tag="ACHGT5_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN7218252.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            435..1958
                     /locus_tag="ACHGT5_002294"
     CDS             435..1958
                     /locus_tag="ACHGT5_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN7218253.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            1982..2221
                     /locus_tag="ACHGT5_002295"
     CDS             1982..2221
                     /locus_tag="ACHGT5_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218254.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2414..2977)
                     /locus_tag="ACHGT5_002296"
     CDS             complement(2414..2977)
                     /locus_tag="ACHGT5_002296"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN7218255.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3070..3288)
                     /locus_tag="ACHGT5_002297"
     CDS             complement(3070..3288)
                     /locus_tag="ACHGT5_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218256.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            3477..4424
                     /locus_tag="ACHGT5_002298"
     CDS             3477..4424
                     /locus_tag="ACHGT5_002298"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN7218257.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            4391..4480
                     /locus_tag="ACHGT5_002299"
                     /pseudo
     CDS             4391..4480
                     /locus_tag="ACHGT5_002299"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4613..5020
                     /locus_tag="ACHGT5_002300"
     CDS             4613..5020
                     /locus_tag="ACHGT5_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN7218258.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(5261..6601)
                     /locus_tag="ACHGT5_002301"
     CDS             complement(5261..6601)
                     /locus_tag="ACHGT5_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN7218259.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6877..7032)
                     /locus_tag="ACHGT5_002302"
     CDS             complement(6877..7032)
                     /locus_tag="ACHGT5_002302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFN7218260.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(7039..7185)
                     /locus_tag="ACHGT5_002303"
     CDS             complement(7039..7185)
                     /locus_tag="ACHGT5_002303"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218261.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(7216..7419)
                     /locus_tag="ACHGT5_002304"
     CDS             complement(7216..7419)
                     /locus_tag="ACHGT5_002304"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN7218262.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(7329..7661)
                     /locus_tag="ACHGT5_002305"
     CDS             complement(7329..7661)
                     /locus_tag="ACHGT5_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218263.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(8175..8366)
                     /locus_tag="ACHGT5_002306"
     CDS             complement(8175..8366)
                     /locus_tag="ACHGT5_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218264.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(8464..8850)
                     /locus_tag="ACHGT5_002307"
     CDS             complement(8464..8850)
                     /locus_tag="ACHGT5_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN7218265.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            9544..>9703
                     /locus_tag="ACHGT5_002308"
     CDS             9544..>9703
                     /locus_tag="ACHGT5_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN7218266.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNI"
BASE COUNT         3159 a         1526 c         1851 g         3167 t
ORIGIN      
        1 agatcaagac ataaaaaaag attttttatt ccaattaatt gaccggctga ttttaaccaa
       61 ccaagggagc ctgtatcgtt ttaaaggatt gattgctttg caagatcaag attttatagt
      121 tgccatacaa ggggtaaacg aacaggttaa ttttcaatac accactcgac aaaaagacca
      181 aaccactatt attctaattg gaaaaatgtt ggatgaagaa aggatcaaac agagttttga
      241 taaattactg gcggaaagca aataatgata agtattgatt aggggtaatt tctaatggag
      301 aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatatcaa aactagaaaa
      361 agggcattaa aaattatcaa agaacataaa caaaataaaa tgaagaaatt agcataatta
      421 aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca gctctaggat
      481 tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa gatggaaaat
      541 tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc gtcggcgatg
      601 aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat gagccctcag
      661 ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac gaaaacatgg
      721 aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag
      781 gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat cacggagaag
      841 aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg gcaattaagg
      901 aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca aaagtatttg
      961 agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac tataccacta
     1021 gtcttaaaga agcgaaacaa aaaagttttg tgacacaaca tgcagccttt ggatatttag
     1081 ctttggatta tggattgata caagttccta ttgctggact aagtcctgaa gaagagccat
     1141 cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc aattatattt
     1201 actttgaaaa gaatgctaat gataaaatcg caaaaacttt ggccaatgaa gctgggataa
     1261 aattggaagt attgaatcct cttgaaagtc ttacaaaaga acaaatggat aatggtgaag
     1321 attatgtttc agtaatggaa gacaatctaa aagcgttaga gaaaacaact atggttgctg
     1381 gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct aatgggtact
     1441 ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa tggcaatctg
     1501 tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa gcaaaactca
     1561 ataaagacat gacagctgcc gagtacaaag attattatac tactggctat aagacagata
     1621 ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt gaacatcatc
     1681 aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg
     1741 ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat attcaattta
     1801 gcgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt ggtggagaaa
     1861 gtcaagaaaa actttataat gaaatgcata attggcctac tttctatcca gcttcattat
     1921 ctgagcatga gattgctcaa gaaatgatgg cccattaaaa tccaatcttt ggaggaaata
     1981 catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt atcggcgtta
     2041 tcaatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg ctgcaacttt
     2101 acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt gttgttttat
     2161 caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct tggccaatta
     2221 attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc tttttattat
     2281 ttcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata aggtgttctc
     2341 caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt tatcgttgag
     2401 ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag tctattcgta
     2461 ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag ggcatattat
     2521 tcaatacttc tgtaactgcg aaatgattgg agaattcttt tcctctatct ggagtgatcg
     2581 ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg ctcacataag
     2641 cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca
     2701 aagtcactaa acaagtacca ctagttttgc cagctacaat atcggctttc caatgaccaa
     2761 aatcacttct atcatctgcg gattttggat ggccatggat cgaatgagta aggcaaatct
     2821 tcccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac
     2881 ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg
     2941 cacggtagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc aaacgattcg
     3001 agattttctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt gaccaaactc
     3061 acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca taattttttt
     3121 ccgctttgga aggtgattag tttttttctt gtttcttctc agttcatgta aaatagtaaa
     3181 cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt cgtgaagcag
     3241 aaagattctt tctcgtttga gtatagtaag atgacaatac tggctcataa ttattttcct
     3301 tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact catttttatg
     3361 tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa aaaataccta
     3421 ttacaatctt aactggctat cttggttcag gaaaaacgat acttctaaat catattctgt
     3481 acggtgatca cggaaaggaa attgctgtca ttgtaaatga cataggggaa gtaaatgttg
     3541 atggggtatt agtcgacaag aaaggtttta aaagaactgg agaaaaactt gtttcgatgc
     3601 cgaatggatg catttgttgt acattacgcg aagatttgat tgttgaacta gatcaattag
     3661 tgcaattttc tgacttgaat caaattgtta ttgaagcttc aggaatcagc aaaccaattc
     3721 caattattca agcaattata ttagctaaaa accctttaga cattgatttg acccaacgat
     3781 tagagattgg tgccattatt atagtagtgg atgtaaaacg tttttgggat aatttcgaaa
     3841 cgggtgaaaa gattcaggaa agaaaagctg aaggtaatgt agttgaagaa gaacaggata
     3901 ttcgtggctt gttaattgat caattcgagt gttgtaacat attactttta aataatagtg
     3961 acttagtgtc aaccaagacg atttacaaaa ttaacggtct aattgctaag ttacagtcca
     4021 atgccaaaat aattaggaca attaaaggtg aagtagactt atcgaaaatt ctaaatacaa
     4081 atctttttaa tatagatgag acttcctatt caatgggctg gttcaaagag ttagagcttg
     4141 gctatcaaaa tcatattcct gaaacagaag aatatggtat ctcaagtttt atctatcgaa
     4201 atcggttact ccttgacgca caaaaatttg cccatcttac ggataaaaat tttcctggca
     4261 atattacaag agcaaaaggg tttctttggt ttgcccaaca tagtgatttt tcgacgatct
     4321 ttgaaacatc gggtagccat aaaagtattg aatcattaaa ttattgggta gctagtttac
     4381 ctaaaattga gtgtcaaatg attatacaaa aaaacccgaa ttgaaggaag cctggcatcc
     4441 gttgtttggt gaacgcatta ataaactagt ttttattggt atgaagatgg atcaacaatg
     4501 tattaaggaa caacttgagg gttgtttaac tgattctctt aatgcagata atttccgaaa
     4561 aatttcacca tttccatgga aaaattctaa tatgtttagc cagatatcgt taatgaaatt
     4621 tttaagaaaa gctggtgtca agataggcga tgtcataatt attggcattt tgattattcc
     4681 ctcttttatt cccttatttg tattctctat agaaaaggag aatgaaacaa ctgaagatgc
     4741 tatcaaatat gccatcgttc aaattgatgg caaggaaatg gatcgcttcg atttggataa
     4801 gatcaatcat aaactcgtga attatcatcc tgcgaaggga caatataata ttgttgagat
     4861 taaagatgga agaattcgag ttaaagaaga taatagtcct gatcaaatcg cggttagaac
     4921 tggatggatc tcaaaacccg gtcaaacgag tatttgttta ccccataagt tagttattag
     4981 tattgagaaa aaagaaagca aagattacta catttactaa tctggaaata gatgaggagg
     5041 tttttaataa gaaaaagata ttattattaa taatagcagg ggctttatta agtgtgttaa
     5101 gcgcttgtgg acaagaaaaa gaagcggtga caacaggcac aagtatcttt aacgggacct
     5161 gcaatggttt agttaacagt cggttagatt acttattttg gctcttcgtc aatgagtgtt
     5221 ggtgaagaaa ttcaatagaa taatcgagat actcacacgg ctagacagct atcttgtcta
     5281 gccgtgttgt tgttctggct ttagtcagtt gggtagtgtt cttagttttc ttgaaatatt
     5341 ttcctcgata taaaaagatt cgttctcggt agttttgaaa gtttctaaag ccataggcaa
     5401 ttcgttttag tactttgata tggttgttca aacattctat agggccgttg gaatagggaa
     5461 ctcgcaacgc tcgtttgatt tctgttcgat atttcttgaa ggtcgtgaac actggttgat
     5521 agtgctctgg caactccttg tagtcttctt ttaggagcgc gacgaattca ggaacatctt
     5581 gtcttctgat agcggaaaga aaaacctggt agacttcgta gcctcgcttt aaggagtcat
     5641 cataatataa tagccgatcg actatctccg cttctgtcag atgatctcga aatgacggac
     5701 gccagtagcg atgctgacca ttcaattccc acgcattctt ttgaagtagt ctccagtatt
     5761 tctttaagtg cttcgaccga cgatcccctt ttttaaaggt cttcattact tgaattcggt
     5821 ggttggtaaa ggcccgaccg atgtgttggc taatatgaaa gcgatcggcg acgattcggg
     5881 cattcggaaa acactctttc accaatgtgc gatagggtgt atagaaatct gaaaccacta
     5941 gacgtacttg ttcacggact tttctagggt agcgcaagaa atagtcgcgt aaaggattga
     6001 gttgccgatt ttccacaata tcaatcagtt gatgggtctt gccgtccatc atgacaaagc
     6061 tcatggctcc cgagacgttt ttcaccgatt tgaattcatc gaaacacacc acttctggta
     6121 acggctggtt caaagaaacg gttttaggcc gaaggctgcg aagtattcga atgacagtgg
     6181 tcggagaaat gtgcttcata cgtgcaatca aagacataga aataggctca cttaatagct
     6241 caagaatggc ttgtttgact ctgcgggcaa tggagcagcg acgatcgcta actgaatctt
     6301 ctgctaaaaa agtctttcca cagtgtttac atttgaagcg ctgtttgtta atccgcacaa
     6361 tggtcttgta gttactgaca tcattcaata agatggatac ttttttccag ccccatttaa
     6421 tgatcgtttg cttattttga tagtggcaat ggaagcattc ctctggttga taggtcaatg
     6481 tgccatcaaa aacaaaacat gtttcgtcat taatgcgctc tttgcgaaag gcatcttcat
     6541 gaaaggtaag atttaggtct agaatatcta gtaactcttt ggtaaactct gaataggaca
     6601 tcttgaaaca ctcctttgaa tggttttcgt cgactttatt ctaaaggatt tcaaggtgtc
     6661 tgtttgcgtt tattagaaaa aagaaaaagt attggcaaca gaaggcgtca ccaacactag
     6721 atattataga tcccttattt tcttcgcttc aaattgtcaa ggagtcatca taaattttgt
     6781 atggattctt ttgtttgggt agtagtcttc taaataatct tggattaaca ccttcatctc
     6841 tattttactt ttatatttgt tggagtcgat taattctcat ttgattgatc gatgaaacgc
     6901 ttcaattact gcattgtcaa caggagtccc agtttggctc attgaatgtg tgatgtggtg
     6961 gtcggtcaac agctcttgat aaataaaaga ccgatgctgg ctacctctat cggaatgaat
     7021 taccgtaggc aattctcctt aataaatttg taatggacgg acgacaatct cttttttcat
     7081 gtgtgaatcc accatatagt aacgtagaat acgagttgct aagtcaatat aggtggacaa
     7141 atagaggcga ccgtcattac atggaatgta agtaatatcc gtcacccaaa cagcatcaat
     7201 ttggttctgt tcaaattatc gattcaacat tttttttacc atttctttga ttgttttttc
     7261 tctaccatga gaagatttgc gtcgatgaaa tcctcttgtg cataaattca tttcacgcat
     7321 aagaacagct acaacacgtt tattgatttc gattccttca tcgtaaagtt tttgagtgat
     7381 ccctggagat ccgtatcgat ctttgtgttc gtagaacact cgtcttcctc ttttagtcgt
     7441 ttattttcct gttcaagaat gtagaaatct gctttatcag gtaatctttt tccttgtcca
     7501 gcaaaagcat ttttcccgta tcgacgatat tctgaaaccc atcgggaaat tgttggacga
     7561 gagatattgt attcttcagc aattttcttg gtagttactt ttttctcaag gatatcctgt
     7621 gaaatcttta gtttaaattt acgatcatat gtttttttca atagagttca ttccttccgg
     7681 taacgtaact gatagtcaag aaaagtgtat catagcccaa cttagtatca gggtttgtca
     7741 actaaactgt ggaagttgat agataagagt ttttaacaac caaaattctc ttgactattt
     7801 ttgaaatcag acaaattttg attggacaca tagttgaata aatctaccgt cgttacggaa
     7861 ggtaaatcgc atgcttttca attctagagg agaagtcctc gttgaagtac catggcccag
     7921 ttctggattc gacctcctgc ccacttcgta cgtaaataaa gtaattttag gagtgagact
     7981 gtgacaaaag ttatttttaa gctaaaaacg aattaaaaaa cgttaataat taaaatcatt
     8041 cgttttagat tctatgaatc attatagaac gctgtaaaat cgatacttta taattataaa
     8101 gaagatggct tcaaatttat tgttcgaaat ttgaaactat ctttttactt atgtcacaga
     8161 ctcccttagt tgagttattc tttaatttcg aatttcgaga tagctagata tattgcaagc
     8221 atacattctt gataacaatc ttgatcatat tttccagttc tccaggattg cttatggacc
     8281 aaagcgctaa atcttttgag aatggccaca actgcttctt catttccatt ctttgctaaa
     8341 atgaaatctt cttttaaagt attcataaat ttaccttctt cccatgaact tataatctat
     8401 ttattttttg gggaaggagg aggacgagca aagaagcata caaaattagt tattatgcag
     8461 atattaaacc agcatttttt gaattttttt gtaaagtcga tgttttaaat ttgtcgcacc
     8521 ctgtcttgta atacccagaa cctttccaat ttctttatca gttttattaa agataaattt
     8581 ttcgataaga aaaatttgtt caggttcttt caacaaagga aggatttttg ctaattttat
     8641 atcttcaaca aggagagtca catttaaaac attaatcggt tgtttaaatt ttatagtatc
     8701 tttagtaaac aaatatggtg ggagttgatc aagttgttca tgtttttgaa tttcttcttg
     8761 ttttttataa taagtattag cggtgttcaa aatgaccttt tcaaagaatc gaatcaactg
     8821 ggcatttacg cgttcgtcgt tatctgacat attgcacacc tcctccatta agatttaaag
     8881 ggaaaagagg caaaaaacac gaaaagaagc taatgcaatt atgctgcgct gcgaataact
     8941 tctataagct cacatgcatt gataggactg ctgatgttta ttttcaaaaa tgttttttta
     9001 gttgataatg gatagttatc tttatactta gaaaagaaaa ttcatatatc ttcttaaata
     9061 tcaaacatgt ccttatggct aagaaaatgt cttgagcttt ttacgtataa taaatctttt
     9121 ctaaaacctc cttttggata atattcttat tcgtgtatag attgtatttt ttatttcttc
     9181 ccccttcata attaacaact agaaaagagt tcgaatagac acgtatttga acgaaaattt
     9241 ttattggatt cccaaattgg agtatttaga aacgaaaaat tcctattatc tgtatagtgc
     9301 ttagataata acggatcata ataattattg tctttgaact agatatatgt aaatagtatt
     9361 catcatgatg aaaaatgatg agttctcggt gataaaatgt actttttacg gtggaatata
     9421 catgaaagcg ataagtggat attttataat gaagtaagtt agcttatttt gacagaggaa
     9481 tagtatttta gataacgtat aaaatactat ttaatgatgt agaaaggaaa gagagaaaca
     9541 aaagtgtttt cagataaaga agtcttatta aagtctcttc atgtttgttt aaagttacct
     9601 atcgtggttt ttaattccga attcagtatt ataaaagagt atcgctcaga tagaactact
     9661 tatttttttt atgattataa aaagttacta actgataata tta
//
LOCUS       DAZBWR010000106         9551 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000106 DAZBWR010000000
VERSION     DAZBWR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.110"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..417
                     /locus_tag="ACHGT5_002309"
                     /pseudo
     CDS             1..417
                     /locus_tag="ACHGT5_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(421..1140)
                     /locus_tag="ACHGT5_002310"
                     /pseudo
     CDS             complement(421..1140)
                     /locus_tag="ACHGT5_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1091..1300)
                     /locus_tag="ACHGT5_002311"
     CDS             complement(1091..1300)
                     /locus_tag="ACHGT5_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218267.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(1282..1350)
                     /locus_tag="ACHGT5_002312"
                     /pseudo
     CDS             complement(1282..1350)
                     /locus_tag="ACHGT5_002312"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            1577..2850
                     /locus_tag="ACHGT5_002313"
                     /pseudo
     CDS             1577..2850
                     /locus_tag="ACHGT5_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2957..3988)
                     /locus_tag="ACHGT5_002314"
     CDS             complement(2957..3988)
                     /locus_tag="ACHGT5_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218268.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3995..4825)
                     /locus_tag="ACHGT5_002315"
     CDS             complement(3995..4825)
                     /locus_tag="ACHGT5_002315"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7218269.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4822..5637)
                     /locus_tag="ACHGT5_002316"
     CDS             complement(4822..5637)
                     /locus_tag="ACHGT5_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7218270.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5634..6458)
                     /locus_tag="ACHGT5_002317"
     CDS             complement(5634..6458)
                     /locus_tag="ACHGT5_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFN7218271.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6448..7548)
                     /locus_tag="ACHGT5_002318"
     CDS             complement(6448..7548)
                     /locus_tag="ACHGT5_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN7218272.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7726..8511
                     /locus_tag="ACHGT5_002319"
     CDS             7726..8511
                     /locus_tag="ACHGT5_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFN7218273.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8544..8927
                     /locus_tag="ACHGT5_002320"
     CDS             8544..8927
                     /locus_tag="ACHGT5_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN7218274.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9003..9134
                     /locus_tag="ACHGT5_002321"
     CDS             9003..9134
                     /locus_tag="ACHGT5_002321"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN7218275.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9103..9339
                     /locus_tag="ACHGT5_002322"
     CDS             9103..9339
                     /locus_tag="ACHGT5_002322"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN7218276.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9351..9437
                     /locus_tag="ACHGT5_002323"
                     /pseudo
     CDS             9351..9437
                     /locus_tag="ACHGT5_002323"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9417..>9551)
                     /locus_tag="ACHGT5_002324"
     CDS             complement(9417..>9551)
                     /locus_tag="ACHGT5_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFN7218277.1"
                     /translation="IELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF
                     "
BASE COUNT         2796 a         2267 c         1850 g         2638 t
ORIGIN      
        1 gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag
       61 atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaaa gcccgtttaa
      121 aggtcaagta cctagaatgc cagctattgt cagctgctta aaacggtctt tccaaatgcg
      181 gagatcgtaa ctgatcgctt tcatattgtc caacatatca atcgcagctt caatcaattg
      241 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      421 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      481 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      541 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      601 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      661 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      721 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      781 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      841 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      901 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      961 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     1021 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     1081 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     1141 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     1201 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1261 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1321 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1381 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1441 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1501 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1561 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1621 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1681 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1741 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1801 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1861 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1921 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     1981 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     2041 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     2101 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     2161 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     2221 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2281 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2341 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2401 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2461 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2521 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2581 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2641 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2701 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2761 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2821 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2881 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2941 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     3001 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     3061 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     3121 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     3181 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3241 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3301 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3361 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3421 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3481 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3541 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3601 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3661 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3721 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3781 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3841 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3901 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3961 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     4021 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     4081 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     4141 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     4201 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4261 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4321 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4381 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4441 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     4501 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4561 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4621 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4681 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4741 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4801 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4861 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4921 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4981 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     5041 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     5101 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     5161 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     5221 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5281 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5341 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5401 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5461 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5521 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5581 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5641 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5701 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5761 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5821 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5881 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5941 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     6001 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     6061 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     6121 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     6181 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6241 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6301 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6361 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6421 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6481 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6541 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6601 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6661 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6721 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6781 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6841 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6901 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6961 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     7021 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     7081 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     7141 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     7201 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7261 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7321 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7381 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7441 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7501 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7561 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7621 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7681 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7741 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7801 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7861 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7921 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7981 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     8041 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     8101 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     8161 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     8221 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8281 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8341 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8401 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8461 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8521 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8581 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8641 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8701 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8761 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8821 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8881 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8941 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     9001 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     9061 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     9121 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     9181 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9241 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9301 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9361 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9421 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9481 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9541 aaaagctcta t
//
LOCUS       DAZBWR010000107         9529 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000107 DAZBWR010000000
VERSION     DAZBWR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.111"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..935
                     /locus_tag="ACHGT5_002325"
     CDS             156..935
                     /locus_tag="ACHGT5_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218278.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            981..1925
                     /locus_tag="ACHGT5_002326"
     CDS             981..1925
                     /locus_tag="ACHGT5_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN7218279.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1941..2720
                     /locus_tag="ACHGT5_002327"
     CDS             1941..2720
                     /locus_tag="ACHGT5_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN7218280.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2732..3430
                     /locus_tag="ACHGT5_002328"
     CDS             2732..3430
                     /locus_tag="ACHGT5_002328"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN7218281.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3458..4222
                     /locus_tag="ACHGT5_002329"
     CDS             3458..4222
                     /locus_tag="ACHGT5_002329"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN7218282.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            4236..6053
                     /locus_tag="ACHGT5_002330"
     CDS             4236..6053
                     /locus_tag="ACHGT5_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFN7218283.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            6065..7030
                     /locus_tag="ACHGT5_002331"
     CDS             6065..7030
                     /locus_tag="ACHGT5_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFN7218284.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVV
                     LVSSEKVIDICENKILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSSSVNTFIV
                     NNEKELLFFSEYIDNPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKG
                     QIIKDRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGAN
                     SPEYIIKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            7023..7718
                     /locus_tag="ACHGT5_002332"
     CDS             7023..7718
                     /locus_tag="ACHGT5_002332"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7218285.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            7715..8833
                     /locus_tag="ACHGT5_002333"
     CDS             7715..8833
                     /locus_tag="ACHGT5_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFN7218286.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            8823..9443
                     /locus_tag="ACHGT5_002334"
     CDS             8823..9443
                     /locus_tag="ACHGT5_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN7218287.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT         3439 a         1527 c         1913 g         2650 t
ORIGIN      
        1 aatagtttgt ggtatgatta tatagggaaa acttgtttac aaatatttct gtgaatatat
       61 taatttaatt atgtttttta aaataaaaag ctgtcatttt tatgtatgtg aattatatct
      121 taatttatct atcatcaata cttaggagtg aatctatgga aaagccattc gtcaagtcat
      181 tgactgctgt ggcggtaggt gcaggtgcta ttgcgatctg tttgtttggt taccatatca
      241 ataatcagcg gcaacatcag caacggatca attatgcaga atctgccatc accaatcaaa
      301 aagacacctt gacttcttta tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa
      361 attttctaaa ccctgaaata acagaagaaa caatcacaag tttgtcaaat aagctatcct
      421 ctgtcaaact ttcagcagat gattacgaca tcaaagaatc agaattgcct aaagaagctg
      481 ttgcaatcca agatgagaag aaagcaatcg tgacacagtt agatagtgca gaatcaaaac
      541 tgaagatcca gacagctgtc aacgatttat ttactaaaaa tgtatcaaac tggcagcaag
      601 ctgtagacga tgtcgtcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg
      661 aaaatatgag cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat
      721 tcgctgatac tcagatcgcg caagtcacgc aactagatca gttattcgat acgatgttga
      781 aggatggtca agtgactgcc accgctactt acgatcaata tttgacggcg ctcagtcaaa
      841 tagagcagat ccggaatgaa aaaataagcg cagcatacgc aacaaaagca caaactgttg
      901 ctcagcaaat gggctatagc aatataagtt attgacagtt aaaacgttga ttcaagcaag
      961 gttagcaaac ggaggaaact atggcaagac gaagaagaaa atcaagaggg aaaaaaatat
     1021 ttctgattat tttagcagtt cttatcgtat tagtattagc aattgtagga gtaggagcaa
     1081 aattatatct ggatctctca cattctattg agaaaactta cgaatcagtg gaacgaaccc
     1141 aaggagaacg aacgaaacca gttgatctgg acaaacaaga atctttctct gttctattga
     1201 tgggcattga tactggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg
     1261 ttgccacagt cagtcctgaa gacaaacaga caacgatcgt cagcattcct cgagatacgt
     1321 atgtcgacat cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac gcattcggcg
     1381 gagctgccat ggcgatggat accctacaaa aatatgtaga catcccaatc gatcactatg
     1441 tctctatcaa tatgaaaggg ctaaaggaac tcgttgatgc agttggtggt atcgaagtca
     1501 ataatgacct cactttctca caagatggct atgactttcc aatcggtaaa attacattga
     1561 acggggatca ggcattatct tattctcgta tgcgttatga agatccaaat ggtgactatg
     1621 gtagacaaga gcgtcaaaga aagatcgtag aaggtgtagc aaaaaaagta ttgagtttaa
     1681 acggtgtcag caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca
     1741 tgagctttga tgaaatgaaa aaaatcgctt tagattatcg ggaggcattt ggtacgatca
     1801 agcaagatca gctgcaagga gaaggcttca tgcaagataa tatctcctat caaaaagtca
     1861 gcgatcaaga acttgatcgt gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata
     1921 aataggaaag tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat
     1981 tacgcaaaca cttggtaaca atcatcatct caatgtttgc tggcttggcc atctcaggaa
     2041 tcgtaacatt tttcatcttg acaccaaaat acagttcaca agcacaattg atcgtgacac
     2101 tgccacaatc agacacagca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata
     2161 cgtataaaga tatgatcgtc agtgatttag tactaaatga agtcaaagat cgtttagaaa
     2221 cagaagacaa tgtgaaaatg actgctggac aaatcaaaga tgcgatctcc gtcaatcagt
     2281 cacaaaattc acaaatgttc tcgatccagg caataagcac gaatgcagtg actgctcaac
     2341 agatcgctaa cacaacttca caagttttcc aagagaacgc aaaagacgtg atgaatgtcg
     2401 ataaaatctc gattatttca ggtgcagttg ccaatacgac acctgtttcg ccaaataata
     2461 aattgaatct agtgattggc ttagtattag gaatgatggt aggcgtaggt ctggcattct
     2521 tattcgaatt acttgatcgg acagtgaaag atgaaaaata tgtgacggat gcattaggat
     2581 tcccaatctt aggaacagtt ccagaaatgt cagcgaaaga attgaacgct ggtatccgta
     2641 aaaaaggaac agctcctgca cgaccaaaag ctacaaacga taaaacagcc ccatcacgca
     2701 gaagccgctc aagagtctag gaggaagaaa aatggcacga acacagaaac aaaaagcagc
     2761 acaaactcat gcagtcagtt tgatcacatt aacaaaccca tcttcaccaa tcgccgaaca
     2821 atatcgtacg atccggacga atatccagtt tgcctccgca gctggtcagc aaatcaaaac
     2881 gattgtcgtg acttcatcag gtcctggaga aggaaaatcc accactgcag caaacattgc
     2941 agtcgttttt gctaaatccg gtcaacgagt tctactagtc gatgcagatt tacgtaaacc
     3001 cgtcgtctat aagacattcc agctgaacaa tgccagggga ttaagtacag cacttagttc
     3061 atcaggaagt gtagcagatg taatccaacg aacgccagtc gagaacttgt cgattttgcc
     3121 tagtggacct aagccaccca acccatcaga attgttaagt tcgccaagaa tggatcagat
     3181 cctagacgaa gcaagacagt tatttgacgt ggtgatattc gatatgccac cagtagtagc
     3241 cgtaacagat gcacagatca tgtcttcaaa aacggatggc actttattgg tcgtacgtga
     3301 aaatacatca cgtaaagaat cattgaataa agcaaaagaa ctgctagaga tggtacaagc
     3361 acgagtttta ggtgttgtat acaacggagc agaacatagc aaagatgctg gttattacta
     3421 cggaaattaa ggaataagaa gagaggagac aagagaaatg atcgatctac attgccatat
     3481 cctaccagga atcgatgacg gggcagaaaa cttagaagca agtattgcaa tggctgaaaa
     3541 agcgattcag caaggcatca cgcatatttt atgcacacca caccacaata atggaaaata
     3601 cagcaatgaa aaatcacaag tcatttctct agtcgcttcg ttacaagccg aattagagaa
     3661 aagacagttg cctttgacgt tattggaagg acaagaagta cgtattaccg gaacgctcat
     3721 cgaagacatc caccgagatg aaattttatt tacagattta gatgatacat atcttctgat
     3781 cgaatttccg acactggaag tgccgttata tgcagaacgg cttttttttg agttgttaga
     3841 actaggaaag acaccagtga tcgtccatcc agaacgcaac gcgcattttc gcgaagaccc
     3901 gaatcatttg cttccgttcc tagatatggg gtgtttggcc caacttactg cgccaagtta
     3961 tgttggggta tttggcaaag atattcagaa aacagctaaa acaatggtca aacataatct
     4021 cgtgcagatg actgcttctg atgcacacgg agtctctaag cgcacctttt acttaaaaga
     4081 gtgctatgag cagattgcca aggactttgg taaggaaaaa gtagtacaga tgaagcaagt
     4141 tgcaaaggat ctgatcaacg gggatgaaat cagttatcca gactatgaag aggtaaaaaa
     4201 gaagaagttt ggtttatttt agggggaatt aaaaagtgtt tatattaaca agaagaagaa
     4261 aaattgccac attggttttt gtggacagct tgttattaat tgctgcaaat attgcagcag
     4321 ctaaattcat gaagccgttt gtatctatac caatggatct tattttaatc tctatcggtt
     4381 tgtcgattgg tttttattta ttttatggta gtttattcaa agttttcact cggatcaatc
     4441 gctatacaaa tttaagagag atcattgcaa tttttagttc actatctgca agtgctgcct
     4501 caagtatcct catactgctg ttcattaatc ggcgatacag tttacggtta gttatctttg
     4561 cttatctgct ttcgctctta ttgatcatcg gtagccgatt gatctggcgt atctacatcg
     4621 aaacaaaaaa tatgagatac gtatcagcag attctgctaa aaatacattg attgttggtg
     4681 ctggcgaagg cgggcgtatt ttatacaaca gctttcttgg atccaaaacg gctcaagata
     4741 tccacgttgt cgggtttgta gatgatgatc caaataaaag aaatacgtat ctttcaggaa
     4801 aaaaagtatt aggtgcatta aaagatattc cggaattgat tgaaaaatat gacattcaaa
     4861 tggtaacgat cgctatccct tccttatcaa gaaaaaaatt acgacgaatc ttcgagttag
     4921 tcgaatccgc tcgtgtgaaa gtcaacacga tgccttctat agaagaactt gcttcaggga
     4981 aaattagcgt atcaaaatta aaaacaattg acgtagtgga tttactggga agagatgagg
     5041 taaagctgga tattgaatcc atcaaagatc agatcacaga caaagtcatt cttgtcaccg
     5101 gcgcaggagg atcgatcggt tcagaaatct gtcggcaaat cattcaattc aatcctgcaa
     5161 agctattgct tttaggacat ggggagaact cgatctactt gattgaccgt gagttacgca
     5221 cgcatcatca aaattgtcct acagaaatcg taccgatcat cgcagatatc caagatcgcg
     5281 agaaaatcaa tgagatcatg gaacaatatc atccagatat cgtctaccat gcagctgctc
     5341 ataaacatgt accactgatg gaatataatc caaaagaagc agtcaaaaac aatattttcg
     5401 gtacaaaaaa tgtagcagaa gcagctaaag cagcaaaagt gaaaaacttc gtcatggtat
     5461 cgacagataa agctaacaat cctccaaatg tcatgggttc aacaaaacga atcgctgaaa
     5521 tgattgtgac tggattgaat gaagaaggct gtacgaaatt ctcagctgtc agatttggga
     5581 atgtccttgg ttctcgcggt agtgtgattc cagtattcag agaacaaatt gctcaaggtg
     5641 gtccaatcac tgtaacagac tttcgtatga ctcgatactt tatgacgatt cctgaagcta
     5701 gtcgtttagt tattcaatct ggcgcattag caaaaggtgg cgaaattttt attctagata
     5761 tgagtgaacc agtgaagatt gtagacctag cgaaaaacat gatccgattg agcgggtata
     5821 gcgaagacga aatcgaaatc attgaaacag gtatccgtcc aggggaaaaa ctatatgaag
     5881 aactattgtt ggataaggaa agaaatgatg aagcagttta tgagaaaatc tttgtaggaa
     5941 acatcaaagg ttactcaata caaaaagtga tggattttgt caagagcctg ccgcaagacg
     6001 atgaacaatt ggctaaagac attgtcactt ttgcaaatgc ttcgaataga taggagatta
     6061 aaaattgaat attttaatat taagttgtgg acgtcgttgt gaactagtga atgcatttaa
     6121 aacagcatta aacaataatg aaatggaagg aaaaatcatt ggagcagatt gctctgaact
     6181 agcacccgca ctatatatgt gtgacgaaaa aatagtatta cctaaagttt cagaaagtaa
     6241 ttatattgag gaattaataa aagtaataaa gttttttgaa attaaattaa ttgttcctac
     6301 tattgatact gaattatcaa cattatcaag aaataaaaaa tatattgaaa atgaaacaga
     6361 ctcagttgta ttagtttctt cagaaaaagt catcgacatt tgtgagaaca aaattttaac
     6421 ttctgctttt tttgaacaaa atggttttaa aactcctgaa attttaaact cttctgaaat
     6481 tacaaaattt cctgtattta taaaacctct gaatggtagt tcatctgtta acacatttat
     6541 tgtaaataat gaaaaagaat tgttgttttt tagtgaatat atagataatc ctcttattca
     6601 agagtatatt ttaggtgaag aatatactat agatgtttgc ttaaactttg attcgacacc
     6661 agtaagtatt gttcctagaa aaagaatagc aactagaggt ggggaaatac tgaagggtca
     6721 gattattaaa gatagagaac taattgagac tacgaaaaag atgttggaaa aattaaatgc
     6781 aatcggacat attactattc aatgtataaa aaacgaaaag ggaatatttt atttagaaat
     6841 aaatgcaaga tacggtggag gagcccccat tagcattaaa tcaggagcta attcccctga
     6901 atatattata aagatgatag attcaaaaaa aaatttgaga tacagagaaa attatttaga
     6961 taaaatgata gcactgagat atgatcaagc gatttattta gatgaaaaag gaaatgtata
     7021 taatgattaa agcggtgatc tttgatttag atgacactct tgtgtctgaa tatgattatg
     7081 taaaaagtgg atacaaatgt gtagctagaa gattagcagt gcaaaaaatt ttcccggcta
     7141 cttttgacga aatatataga gtccttttag atctttttgc tgaagatagt aaaaatgtat
     7201 ttaatcgtct atatgactat tttgaaattc cttatagtaa aaaagaaatt aaagaactgg
     7261 taaatgtata tcgagaacac aaaccagaca taaatttttt agaaaatgca gatactttat
     7321 taacggaatt aagaagtaga ggatacaagc tggggattat tacagatggg tatgctataa
     7381 cccaacgaaa caaattggat gctttgaaag tagactattt ggtagattat atttgtatta
     7441 ctgacgaatt aggccgtgaa ttgtggaaac cacaccctat ctcatatctt ttattgttag
     7501 aaaagtttaa tttaaaggca caagagtgta tatatattgg cgataatgag ttgaaagatt
     7561 ttataactgc caaatcaata ggaatgcaaa caattaaaat agaaagagta aatggtattt
     7621 acagagaaag cagtttgaag ttcaaaagat tttaccaagc tgataaaaaa atacgttcgt
     7681 tatgggagat aaaaaatttt ttgggagtga tttcatgaaa agtaaaagaa ttctattagc
     7741 ttctccacat atgagtgaag aaggatatga aaaagagtat gtaaaagaag catttgatac
     7801 aaattgggtt gctcctttag gtgcgaatgt gaatggattt gagaaagaat tagcagaata
     7861 cgtgggagct agtcatgctg ccgcattatc ttctggaaca gcagctatac atttagcttt
     7921 gaaagcggct ggggtcggta aagatgacat agtgatttgt cagtccttga cgttctcagc
     7981 tactgctaat ccaattctct atcaaggagc tatacctgta tttgttgata gtgattacga
     8041 aacttggaat atgaaacctg aacatttgcg aacagcacta gaaaaatatc ctaacacaaa
     8101 ggctgtatta gtagtacatt tgtacggttt atcagctaat ttagacgaaa ttactaaaat
     8161 atgcaaagaa tacgatgtta ctctaattga agatgctgca gaatcattag gaacaacata
     8221 taaaggaaaa tatactggta catttggcga ctatggaatt tattctttta atggaaataa
     8281 gataatcaca acttcaggtg gcggaatgct tgtatccaat gacaaagaaa aggtggaaaa
     8341 agtgagattt tgggctacgc aatctcgtga gaaggcacgc cattatcaac acaatgaaat
     8401 tggctataat tatcgaatga gcaatatcgt tgctggtatc ggtcgaggac aattgaaagt
     8461 attaaatgaa agaattgaga aaaaacgata tattcatagt ttttataaaa aagaattgtc
     8521 taaattgtca aatatacaat tcatgccaga aaataattgg aatgaaccga attattggtt
     8581 aagtgtcata acaataaagg gaataaatgc gaatcatctt attgatgagt tagacaaaaa
     8641 taatatagaa gcccgcccag tatggaaacc tatgcattta caacctgttt ttaaaaatta
     8701 tgattatatt ggcgatgatg tagcagaaac tttatttgac ttaggagtat gtttaccatc
     8761 agatacaaag atgacagatg aagatttaaa atatgtagta tctacgatta aaagagtgat
     8821 caatgatgcg taaaaaaact ttctatcagc tttttgggaa aagaatttta gatatcctat
     8881 tgtcaggaat tgctctcatc gttttatcgc cgataatctt aattgtaggt tttttagtaa
     8941 gaattaaact tggttctcca attatcttca aacaagaacg accaggaaaa agtgaaaaaa
     9001 ttttttccat gtataaattt cgtacaatga ctgacgaaag agaccacaat ggagaatatt
     9061 tacctgatga aattcgacta actaaatttg ggaaaatgct tagagcaaca agtctggatg
     9121 agctacccga actttggaat attttgaaag gtgatatgtc gatcgtagga cctcgtccat
     9181 tattagtgga gtatttgcca ctatatagtg agaagcaacg gaaaagacac aacgtaagac
     9241 caggattaac tggactggct caggtcaatg gaagaaatgc tattagttgg gaagaaaagt
     9301 ttgatttaga tgtttattat gttgataaaa ttagtttctt taatgatctt attattataa
     9361 ttcagacatg taaaaaagta atcaagaaag agaatattaa tacaatcaat aacaatatcc
     9421 cagaaaaatt taaagggagt taaatacagc agcttaatgc aagttttgtg acttgcatta
     9481 tatttaggta taaatcaaag cgagtatatt agaattatat aacatatgt
//
LOCUS       DAZBWR010000108         9408 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000108 DAZBWR010000000
VERSION     DAZBWR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.112"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACHGT5_002335"
     CDS             168..362
                     /locus_tag="ACHGT5_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218288.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACHGT5_002336"
     CDS             631..930
                     /locus_tag="ACHGT5_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218289.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACHGT5_002337"
     CDS             complement(1027..1461)
                     /locus_tag="ACHGT5_002337"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7218290.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACHGT5_002338"
     CDS             complement(1693..2403)
                     /locus_tag="ACHGT5_002338"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN7218291.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACHGT5_002339"
     CDS             complement(2393..3253)
                     /locus_tag="ACHGT5_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN7218292.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACHGT5_002340"
     CDS             complement(3258..3896)
                     /locus_tag="ACHGT5_002340"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN7218293.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACHGT5_002341"
     CDS             complement(3911..4210)
                     /locus_tag="ACHGT5_002341"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7218294.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACHGT5_002342"
     CDS             complement(4253..5737)
                     /locus_tag="ACHGT5_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN7218295.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACHGT5_002343"
     CDS             complement(5757..6218)
                     /locus_tag="ACHGT5_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7218296.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHGT5_002344"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACHGT5_002344"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN7218297.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACHGT5_002345"
     CDS             7521..8291
                     /locus_tag="ACHGT5_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN7218298.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACHGT5_002346"
     CDS             complement(8564..9391)
                     /locus_tag="ACHGT5_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218299.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2919 a         1927 c         1481 g         3081 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttccctt
//
LOCUS       DAZBWR010000109         9109 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000109 DAZBWR010000000
VERSION     DAZBWR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.114"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..473
                     /locus_tag="ACHGT5_002347"
     CDS             <1..473
                     /locus_tag="ACHGT5_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFN7218300.1"
                     /translation="EEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQANTIDDLV
                     FLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSAAKAEEIA
                     IVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEITIQF"
     gene            537..4169
                     /gene="pglX"
                     /locus_tag="ACHGT5_002348"
     CDS             537..4169
                     /gene="pglX"
                     /locus_tag="ACHGT5_002348"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFN7218301.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            4285..6807
                     /gene="pglZ"
                     /locus_tag="ACHGT5_002349"
     CDS             4285..6807
                     /gene="pglZ"
                     /locus_tag="ACHGT5_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFN7218302.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            6925..8958
                     /gene="brxL"
                     /locus_tag="ACHGT5_002350"
     CDS             6925..8958
                     /gene="brxL"
                     /locus_tag="ACHGT5_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFN7218303.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         3289 a         1230 c         1794 g         2796 t
ORIGIN      
        1 cggaagaaaa acaagcaaca tttaaattat tagaaggaaa agattttaag gatcaatttg
       61 taccaatagt tgaccaagaa tttcaagaac tgaaagcaaa aattgaacaa gcgaatacta
      121 tagatgatct tgttttctta tcattaaacg tagcagaaca gtatttgatc tttaatcaac
      181 gtttcgataa tgaagaaaac cgcagacgtc aagaagaaca agcacgtaaa caaattgaag
      241 cagcaaactt gattcgtgaa actgaaaaag atgaaataga ctctgcagca aaagctgaag
      301 aaatagcgat agtaaaacct actattcaac agaaaacagt accaatgaga acacttaacg
      361 ttcatgttaa aactattaag tcagcagcag atattgatga gttaacagct gaaattaatc
      421 aaaaactgaa ggaacaactt caagaaaata cagaaatcac gattcaattc tagtgaaaag
      481 acaggcataa aaaggtgcct gtcttttcaa aggttacata tagaagggaa agaaaaatgg
      541 ataagaaagc gataaagaat ttcgcggtca atgcccgtag aaaattaatt gatgaagtaa
      601 tgactaaagc aaaacgttta ggaattgatg aatcaaaaat tgaagatgtg aagaaaattg
      661 atagcaattt acaagaaatc gaaaaatcag gaataagaat tcaaggaaaa gaaatagcgc
      721 aaagaaacaa attgattgca gaactaaaac aacgtgcttc tgtcacttct tattcagaag
      781 cttttcaaga gttaatcgaa gaggttgctt atacgtggtt taaccgattg attgccattc
      841 ggtttatgga ggtaaatgat tatttacctg acacgtatcg tgtattatct agtgaaataa
      901 gtgggaaaat tgagccagat attattacag atatttatga tgccgatatc tttgaagaat
      961 tatctagtaa tgaacagcaa caagtcagaa cttggaaaac agataattca gcagaagcaa
     1021 tggacaagct gtatcaattg atttttataa aaatctgtaa tcaattgaat gattacttac
     1081 ctgagctatt tgaagaagta gaagattata cagaactttt gtttacagct tcttatatta
     1141 aaaaagatgg tgtaatcgct gatcttttaa gtattccaga ggatgatttc tatgttcaag
     1201 ctggtggaca ggtagagatt attgggtggt tgtatcagta ctataatagt gagccacata
     1261 atcaagtggt gaatatttat aaagggattg tcaaaaaaca agatattccg gcagcaactc
     1321 aactatttac cacagattgg gtcgttcgtt atatggttga caatagttta ggaaagtatt
     1381 ggttagatca ttatcccaat agtgaattaa gaaataagtt gaaatactta attccagata
     1441 aatatgcttt tgaacaagca acatctgtaa gtaatttaaa accagaagaa ctaaaaatta
     1501 tcgataacgc aatggggtct gggcatattt tagtttacgc cttcgatgta ttgatggaaa
     1561 tttatcttac gttaggatat tctgagcgtg atgctgcacg tgcaatcgtt gaatacaatt
     1621 tatatggtct tgaaatcgat aaaagagcgt atcaattgtc ttattttgca attatgatga
     1681 aagggcgtca atacgatcgt ctttttttga aaaaaagtct atcgccgaat ttaagagagt
     1741 ttattgattc tcctaaagtg acagacgagt atcaagagcg tattagtgaa ttgaacaatg
     1801 ataagtatag tgaacgctgg aatgaataca aagagttaat ttttgatcaa tttgaaaatg
     1861 ggaaagaact tggatcaatc attaagttag tcgataaaaa taatcaggca ataactaaag
     1921 aatacttagg aaaagtaaga gagtatatat caaaattcaa cgaatttacg aatatggata
     1981 ttctttatgg gatgaaggaa atacaagaaa aatacttgca cattcttgac gtggctgaaa
     2041 tgctagttga taaatatgta gcggtagtca ctaatccgcc ttatcttaat aaaatggata
     2101 agaaattaaa aaaatatgta aatgataact attctgatgt caaaaaagat ctattttctg
     2161 tgtttattaa attgaatagc caaatgctga ttgataatgg atatgcttcc tttatgacgc
     2221 catttgtttg gatgttcatt tctagctatg aaaagttacg ctcattttta attaatgaaa
     2281 gaaacatttc tagtttgatt caaatggagt attcagcttt tgaagaagca accgttccaa
     2341 tttgtacgtt tacgataaaa aattcaaatg aacaagatgg aagctatata aaattatcag
     2401 attttaaagg tggaatggaa gttcaaaaaa ttaaaacgtt agaagcaatt catcagcatg
     2461 gtgaatgcga ttatttttat agtactaatc aagacaactt caaaaaaatc ccaggaatgc
     2521 cgattgctta ctgggcaagc gataacgtaa tcactgattt tgctcaagga aaattaacta
     2581 acgatgttgc agatgcaaaa caagggttgg caaccggaaa taacaataaa tttttgcggt
     2641 attggtttga agtagaattt aacaaaataa agtttgatgc taaaacatta gaggacgcta
     2701 aaaagtctag aaaaaaatgg tttcctacaa ctaaaggtgg tcgttttaga aaatggtatg
     2761 gaaacaatga atacgtggtt aactgggaga atgatggaga tgaaataaaa aattatgtta
     2821 atgaaaatgg gaagttaaaa tctagaccac aaaatcttaa ttactatttt aaaaagggaa
     2881 ttacttggtc gacactttca tctggacaag tatcttttag attgcagaat caggcaattt
     2941 ttgaaactaa aggatctgtt ctgttctcaa aggatataac tgactttgaa gaaatatttg
     3001 gctatgtaaa ctctaaatta gttaatcact ttttaacttt tatttctccc accttggatt
     3061 accatgaagg accaatggga aaactacctt ttcttaaagt tacatctgag agaaaattaa
     3121 aaaaaataag agaaaaagtg gatgaaaata tcgatatagc tagaaaagac tataatgctc
     3181 ttgaaacgtc atacgagttt agaagaaaca ttttgttaag ttatatgttt aaaaaagatc
     3241 tctcagaggc atttaactgt ctaagtaggc agttggttaa tcaatttaat actctaaaat
     3301 cgaatgaaga ggaactgaac gacatattca ttaatcatta ttctcttgaa aaagaattaa
     3361 caaaagaggt tcaagatgaa tatatctctg caaggttacc tgataaggcg agggaaatta
     3421 aggattttat ttcttattca ataggttgta tcttcggtcg ttactcccta gacgaagatg
     3481 gtctcatctt cgctggcggc gaatgggatc cttcccgcta tcaaaaattt aaacctgata
     3541 ctgataatgt tttactcatc accgatgatg agtatttcaa ggatagccaa attgacattg
     3601 tcaatcgttt cattgatttc gttgagatta cttttggtaa agaaaccctt gaagaaaatc
     3661 tccagtttat cgctgacact ttaggtggca aagggacaag tcgagaaatt attcgtcgtt
     3721 acttcgtaca agatttttac aaagaccatt taaaaacata ctccaatcga ccaatttatt
     3781 ggcaattgga ctcaggaaaa caaaatggct ttaaagcgtt aatgtacttg catcgctata
     3841 caccagatca attaggaaaa gtacgtactg aatacctaca cgaactacaa aaagcgtatg
     3901 acaatcgaat cgaattgcgt cagcaacaaa tttcaacaag tgataaccca aaagaaagaa
     3961 atgcaatgac aaaagaaatt gtaaagattc agaagcaatt aaaagaagtt cgtgagtatg
     4021 acgagaagtt gggtcactta gcattaaaac gaattgaatt agacttagat gatggtgtaa
     4081 ttgtcaatta tgataagttg cagcgtgatc ctaaaaccgg cgagaaattc caaatttttg
     4141 caaaaggggt caaagaacca aaaaaatagt tatctaagaa agttgttttc taacaacttt
     4201 cttttttatt cttaaattgc gtgcgaaatt atccaaatta tgtcaatata aggggtatag
     4261 aaagagaacg attaggtgag attgatgaca gatataaata cgcaacaaat tgaagaaaaa
     4321 ttaaatcaac gttttcctaa ttatggatgt aggaaaatca ttttttggtt tgattcaaat
     4381 caggattttc ttgaagaaat tgactcattg gtcattcaaa atgcaaaaat ctatcgatta
     4441 gaaaaggacg cacaatttaa aactaagcgt ttattggaat atgaagatcc cgaaactaac
     4501 tatttaatct atgcgccatt tcctcgttgg gatgataaag atgaagataa tcatttgttg
     4561 agtgtattga attattcaga ggaatttagt gcagaccgct tagcgattat catgaatgaa
     4621 ttggctattc cacttagatt tcatcagaca gttaaacagt atcgtaattt ctttaatgca
     4681 aaggatcgca tgagaagttt tggtgaattt agtacgcata tgacaattaa taaatcagaa
     4741 acgattgaat taatgataat ggctgttttg ttgaaatcga aaagtattcg attaaatgat
     4801 ttactgcgtg tagttctaaa aagctttaca gaaagtaggg agaatccttt aagggaactt
     4861 gataagtatc agttaacagc taaattttgg gaatttattc aaatgaaatt tggctttacg
     4921 gatagccagc caacattgga aaagctgacg atgtgtcttt atttgaacta tttttattat
     4981 caaattgatc aagagatgcc gaagaagttc aaaggatttg aaatactaca tttgaaagca
     5041 aacattatta ctttcatgga tcagtttatg aatgatacac gttacatgga aagctttgaa
     5101 cagctatcta aaaaagtata tcaggctatt gatggtgatg gattattaga cagtatagaa
     5161 attgatgatc taattgaagc agacgtattt tctgagattc atgcaaaaat attaatgttt
     5221 tatgcagaac gattaaaggc ttctgacact acaacgaaaa ttgcaggaca aacaattttt
     5281 gatagtttaa agcagcgcga aaaaatgcat ttcgcatcgg attatattta tgagtatcaa
     5341 ttattgaaac atgcttatta tcttgtggat aaaaacagat tgtgcttgcc gatgaacatg
     5401 gataatttgc taactcaata tgagaaggaa atgtattggg ttgatacgca ttatcggaaa
     5461 tttatttgga atagtgatca tatagaagaa aagcaaaaat atggccaatt gtatcgatta
     5521 gttgaagcaa attattcgaa gtttcttgat caatcaggac aaatttggaa tcaaactttt
     5581 gaatttaaga atcgtccatc cattcgaaaa ttttataatc aagctgtaat gaaacgctct
     5641 gttaaaactg tagttattat ttcagatgct tttcgatatg agttagggat gaatttagaa
     5701 cgtaagttag ttagagaaaa gaagtattca acaaacatgg ctagctttct ttctgtatta
     5761 ccttcagtta ctgaattcgg taaagccgcc ttattaccaa acaatcaact aacttacaat
     5821 gaaggaacag aagtactgat tgatgggaaa aaaacgaatg gtttatccaa tcgaaatgcg
     5881 attctccagc aacgagatgc aaatgctgta gctttaaatt atgatgatgt ggttaagatg
     5941 aataaggctg aggtacgaga agtatttaat ggcaaatcag tcatttatat ttatcacgat
     6001 caagtggata gaacaggtga tcactcgaat gaaaatcgag tttttgaggc ctgtgattta
     6061 gcgataagcg aaatttcccg acttattaat cgcttgcata atgatggcag tatctatcgt
     6121 tttatagtta cggctgatca tggatttatt tataaaagaa gccacgttga agaagtagat
     6181 aagattgaaa atccatctag aaatgaaaaa gatcgtgtag aacgtcgttt tattcttagt
     6241 gaagaagaat acgaaataat tggtacgaaa aattactctt tgggagattc tttaggaaat
     6301 gatgacacta gaaacatttg tgtcccgatt acttcaagta tttttaagaa agctggcggg
     6361 ggacaaagct acgttcatgg agggtcatct attcaagaaa tgctagtccc tgttctagaa
     6421 attacggtag ccaaaggtag caatgcaaag gaaccagtag caattgaatt aatgactagc
     6481 aaccgcagaa ttacaggatt atcaaccacg ttagaattct atcaaaaaaa tgcaattagt
     6541 gatgtttatg accccgcatc tttctggctt tattttgaag atgaatatgg tgaacgtatc
     6601 tcaaatgaag aaatctatgt tgcggattca aaaacagaag atgcaaatca gcggtttact
     6661 aaatttgttt ttgaatttgt aaatcgtcga tatacagttg aagatactta ttatttagtg
     6721 ataaaaaatc aaaaaaatga tttgaccgag cgaataaaat tcattataga taatcctttt
     6781 gcagaatcat ttgattttga tatataaaaa cgaggtatcc acaatttttt gtggatactt
     6841 tttcttttgt attatcaagc ctactggtta agtatgatat actttaattg ctattataat
     6901 gactgaacac atggaggaat tctagtggaa caacaggaaa ttttaaagtt atctgaattc
     6961 tttcccggaa agattgtcag aaaagattta acaaagaaaa ttaaagaagg tgcgaatgta
     7021 cctgtttatg tattggaata tttacttggt atgtatgctt cgtcattgga tgaaacattg
     7081 gttgaaaaag gtgtggaaaa tgttaaaaat attcttgctc gaaactatgt gcgtcctgat
     7141 gaagcagaaa aagtaaaagc aaaaattcgt gaattgggtg gttatacagt tattgataaa
     7201 gtaactgtac atttaaatga aaagaaagat tgttatgaag cagaattctc taacctggga
     7261 ttaaaaaatg ttcaaattag tgaagagtat ccgaaaaaat ttgatcgatt attaggtggg
     7321 gggatctggt gtattgtttc gatggaatat tggtatgatg agaatgatcg ttggaatagc
     7381 ccttttattg caaaaaaggt tgatccaatc caaatgcctg gtttagacat tgaggcttac
     7441 aagatggctc gcaaggagtt tactacaaat gaatggattg acttacttct acggtccatt
     7501 ggtatggaac ctacacaatt taataaaagg gtaaaatggc ttcatttagc aagacttatt
     7561 ccattaattg aaaataattt taatctctgt gaactaggac cacgtggtac aggtaaatcg
     7621 catgtatata aagaaatttc accaaactca atccttgttt caggtggaca aacaacagta
     7681 gcaaaccttt tctataatat ggctagtcgt acaatgggac ttgtaggtat gtgggactgt
     7741 gttgcttttg acgaagttgc tgggattaaa tttaaggata atgatggcgt tcaaatcatg
     7801 aaagattaca tggcttcagg atcttttgct agaggcaaag aagaaaagaa tgcttcagca
     7861 tcaatggctt ttgtaggaaa tattaatcaa agcgttgatg taatattgaa aacttcgaat
     7921 ttatttgagc catttccgga ggttatgggg acggattcag cctttttgga tcggatgcat
     7981 tgttatttac caggttggga aattcctaaa tatcgaccag atttttttac aaatgactat
     8041 ggcttcatta cagattattt ttctgaagtc atgcgtgagc ttcgaaaagt tagttttggc
     8101 gatagttttg ataaatattt caaacttggt tctcaattaa atcagcggga tacgattgca
     8161 gttcgtcgga ttgtatctgg gatgatgaaa ctgttgtatc ctgatggtag atttgaaaaa
     8221 agtgatgtcg aagaaatact aagatttgct cttgaaatgc gtcgtcgtgt aaaagaacaa
     8281 ttaaagaaaa ttggtggaat ggaattttat gatgtcaatt tttcatacat tgatttagaa
     8341 gaagagcgag aagagtatgt ttctgttcct gaacaaggtg gaggaacttt gattccagaa
     8401 gggttaggga agccagggca tttgtacacg gtatcttatg gcaaaactgg gcaattaggt
     8461 acctataaat tggaaacgca aatgacttcg ggaaatggaa agtttgaacg tactgggatc
     8521 aattcagatg ctggagcaag agaagcgatg gattcggcat accgatattt aagagctaat
     8581 tatggaagtg ttagtcagtc tattagtttg acgacaaaag attttgtcgt tcatgttcaa
     8641 gatatgaatg gcattggtat gacaaaaaca ttatcgttat caactataat agctttatgc
     8701 tcagtttcct taaataaacc tgtattacca agtttagtta ttttagggga tattacaatt
     8761 ggtggttcta tgattaaggt agataattta gctaactgtt tacaagtagc aaaagatgcc
     8821 ggagcgaaaa aagtacttgt acctatgata agcgctgctg atattggaac tgtaccccca
     8881 gaattaatgg gtacctttaa tttgattttt tatcaaacgc cggaagatgc tgtctttaaa
     8941 gcccttgggg tggaatagac aacttttcca tttgctggtt tgactacgta ccaatctata
     9001 ttacttacta gattagtcgt taatctattc ggatcgaatg ggcaaaatat ttttgtgtat
     9061 gtccgtcctt gatcatgatt ccaaaaagaa tcattggggg ctgtggcgt
//
LOCUS       DAZBWR010000110         9043 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000110 DAZBWR010000000
VERSION     DAZBWR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.115"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACHGT5_002351"
     CDS             97..540
                     /locus_tag="ACHGT5_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN7218304.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACHGT5_002352"
     CDS             complement(546..1730)
                     /locus_tag="ACHGT5_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN7218305.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACHGT5_002353"
     CDS             1998..2696
                     /locus_tag="ACHGT5_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN7218306.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACHGT5_002354"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACHGT5_002354"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFN7218307.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="ACHGT5_002355"
     CDS             complement(3506..4039)
                     /locus_tag="ACHGT5_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218308.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACHGT5_002356"
     CDS             complement(4336..6717)
                     /locus_tag="ACHGT5_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN7218309.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHGT5_002357"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHGT5_002357"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN7218310.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACHGT5_002358"
     CDS             7418..7957
                     /locus_tag="ACHGT5_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN7218311.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHGT5_002359"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHGT5_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN7218312.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACHGT5_002360"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHGT5_002360"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
BASE COUNT         3009 a         1589 c         1838 g         2607 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttt
//
LOCUS       DAZBWR010000111         9010 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000111 DAZBWR010000000
VERSION     DAZBWR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.116"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(149..562)
                     /locus_tag="ACHGT5_002361"
     CDS             complement(149..562)
                     /locus_tag="ACHGT5_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN7218313.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            complement(638..934)
                     /locus_tag="ACHGT5_002362"
     CDS             complement(638..934)
                     /locus_tag="ACHGT5_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218314.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            complement(934..1140)
                     /locus_tag="ACHGT5_002363"
     CDS             complement(934..1140)
                     /locus_tag="ACHGT5_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218315.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            complement(1137..1334)
                     /locus_tag="ACHGT5_002364"
     CDS             complement(1137..1334)
                     /locus_tag="ACHGT5_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN7218316.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            complement(1358..1663)
                     /locus_tag="ACHGT5_002365"
     CDS             complement(1358..1663)
                     /locus_tag="ACHGT5_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218317.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(1660..1833)
                     /locus_tag="ACHGT5_002366"
     CDS             complement(1660..1833)
                     /locus_tag="ACHGT5_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218318.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            complement(1830..2399)
                     /locus_tag="ACHGT5_002367"
     CDS             complement(1830..2399)
                     /locus_tag="ACHGT5_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFN7218319.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            complement(2411..2545)
                     /locus_tag="ACHGT5_002368"
     CDS             complement(2411..2545)
                     /locus_tag="ACHGT5_002368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218320.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            complement(2545..2880)
                     /locus_tag="ACHGT5_002369"
     CDS             complement(2545..2880)
                     /locus_tag="ACHGT5_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218321.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            complement(2877..3263)
                     /locus_tag="ACHGT5_002370"
     CDS             complement(2877..3263)
                     /locus_tag="ACHGT5_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN7218322.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            complement(3260..3577)
                     /locus_tag="ACHGT5_002371"
     CDS             complement(3260..3577)
                     /locus_tag="ACHGT5_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFN7218323.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            complement(3577..3882)
                     /locus_tag="ACHGT5_002372"
     CDS             complement(3577..3882)
                     /locus_tag="ACHGT5_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218324.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            complement(3879..4040)
                     /locus_tag="ACHGT5_002373"
     CDS             complement(3879..4040)
                     /locus_tag="ACHGT5_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN7218325.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(4055..4414)
                     /locus_tag="ACHGT5_002374"
     CDS             complement(4055..4414)
                     /locus_tag="ACHGT5_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN7218326.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(4411..5262)
                     /locus_tag="ACHGT5_002375"
     CDS             complement(4411..5262)
                     /locus_tag="ACHGT5_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7218327.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(5279..6112)
                     /locus_tag="ACHGT5_002376"
     CDS             complement(5279..6112)
                     /locus_tag="ACHGT5_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFN7218328.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(6119..6805)
                     /locus_tag="ACHGT5_002377"
     CDS             complement(6119..6805)
                     /locus_tag="ACHGT5_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN7218329.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(6811..7557)
                     /locus_tag="ACHGT5_002378"
     CDS             complement(6811..7557)
                     /locus_tag="ACHGT5_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN7218330.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(7550..7891)
                     /locus_tag="ACHGT5_002379"
     CDS             complement(7550..7891)
                     /locus_tag="ACHGT5_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218331.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(7949..8077)
                     /locus_tag="ACHGT5_002380"
     CDS             complement(7949..8077)
                     /locus_tag="ACHGT5_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218332.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(8070..8777)
                     /locus_tag="ACHGT5_002381"
     CDS             complement(8070..8777)
                     /locus_tag="ACHGT5_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFN7218333.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            8822..>9010
                     /locus_tag="ACHGT5_002382"
     CDS             8822..>9010
                     /locus_tag="ACHGT5_002382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218334.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLF"
BASE COUNT         2300 a         1996 c         1359 g         3355 t
ORIGIN      
        1 ctatcgaagc ttaattgtga aacaataata aaacgatgtt ccttttatta ttattttgtc
       61 tcagacctat cactaatctt tcgacactac cataatatca ctggtaaata gctaaaaacc
      121 gccatcattc cgccaaaaaa ccgccaaatt atttataagc aattattctt ccgtgtttat
      181 atgcttctgc aaactctatt agagcttccg acttcatccg ttgtatactt ctttctgaat
      241 aacccacttc acggctaatt ctgtagtttg agaagctatc tggcacacaa aagctgtagt
      301 agagtatctg acggctaatc agactaagag ccatcaaagc cgctaaaatc gcatctctct
      361 ctgcttctat atccatcatc tgaatgatcg cgtcctctgc cttattgccg tgcttcggcg
      421 ccttcggcat atccgtaata attggagact taatatctat caaagagcga cctgccatcc
      481 gctccaaacg ccgaaagttc ttcagcacat ctctcgcatt acatcttgtc tgtttgaaat
      541 ctacctctcg taacaattgc atcaagtcaa accgctcctt tatgtgatat aataaacttg
      601 tctgttttat tatgttagtc ggagcgatcc ggctttttta tttgctctcg attacttcca
      661 tgtcaactaa tctcgctaca gctaaattct ctttgctttt cgctaaccac ttgtcacatt
      721 ccatcgtgtt ttcaatacga atgattgctg agttcgacaa taccttttct acatatccac
      781 gaaatggata gataaatcct tctgcttcac agcgaaccat gtcaccgact ttgaattttg
      841 gtttctcacg tgttttaggg ttctttgtcg gcatatctag catcaaaccg ccgataccat
      901 gactactagc gtaaaatccg tcttttagtt tcattactct accccctcat gatcgacagt
      961 gaccagttca tatacttgcg ttaatccacc aagtcgtcgt gaaacttggt tagcttcttc
     1021 gagattatca aaccatcttg cgctttggag tatgtctaca atggataatg tgttagcccc
     1081 tatagaacgt tcatcgcttc tgtaaaattg atttccaaac tttactacat aaatcttcat
     1141 tctgtatcct cccatttaat atctagtatc gaaattccaa acttacgaat agcatcactt
     1201 gcatcagcaa cacactgact tgccacttta tatgtttctt ctgcagaaac accatactcc
     1261 cgttcgaact ttactttcgc tacattcagt tcttgatttc ttagttttgc tactctgcgg
     1321 tgtctgttgt tcatttttct tcacctctgt tttgttatca ccatttcttt gttgaaaatc
     1381 cacaacgtct atactgttta attcgtttct taggcctttc tagatcaatc agaataaatc
     1441 tgtcggatat atgataatct tctattacag ccgtatacat tggattcgga accgagtacc
     1501 atgatctttt tttatcaaac tcatccgcat catctcttgt aatttctatc atgttagcag
     1561 acattagata gccctcttct tctgcctcgg atgggtggga atttgtatcc cagcttcgag
     1621 gaaacctggc caaatctctt tttaaataaa cttgaacatt cattccgctt cctcctcatc
     1681 gatctctatc tcgacatctc tgccttctaa aatctgtttg gtatcaatgc tttttgtttt
     1741 caacgccaac ccaccgtctt tttgtgcagc aacaatggta atcgtcattg cctttgttcc
     1801 gtctggtata tggagcatta tctgtctact catccttccg ccacctcttc cactggcaca
     1861 gcaaatggcc agtatctttc atcaattgct ttgatctgtt tttcggtcag ttggtagtgg
     1921 tgtgcaaggt attgtggcag gtaagctgtt gatactagtt taacttcatt actgcaactg
     1981 atctccttga caaggtactc gccagcaatt attacactgt acaacggctc tttctcgacc
     2041 tcgtagccgt tgtacaagct caatagtgtt tcatatgatt gtctcgttgc ccaagcgagt
     2101 aattcttctc cttgttcttg cgtcactatg ccatctcgta caaaccaatc acagaaataa
     2161 tatgaatcac catctttaga tttgattaaa tacgctattt tttcagcttt cgtaaaaggg
     2221 tccgtagatt tctcaagcca accggccacg agttgtggaa taactagctt cttcggttcg
     2281 tctatttttt tcattaacgt aatacctgca tttatactct cgttatagca agcagctagt
     2341 gcatcatttc ctatcacttt aatactttct aactttttga ttgcttcctg tttattcatc
     2401 gctgttcctc ctaaaaaatt aactgccaaa gcaatataat cactttcaat gatacaaatc
     2461 ccaccagtgc aataattaca gatataaacg cacagtttat gaaacagcca actaaggcgc
     2521 ttgcttcttc taaccagtct ttcattactg ttcctccttc aaccataaat ttggaaaatc
     2581 atcggcatat atctctattg cgccaaataa cttgatcgct gattttgatt ttccatgtat
     2641 gtcaggactg attaaacgat cataataatc ctgactgatt gtttttccct tgtactgata
     2701 acgtgtagcc gcagtgtaat atactgctgt cttgctcaga taaagatatt ttgctaaatc
     2761 agccgatatc cgtaacaaaa tgattctatt cttactttta attccacaat ttagcaatgc
     2821 tgggacattc ccattttcat cttttaaaga atcgatgtag tcagtagcta agataatcac
     2881 tctacctcca ataactcgct attctcgtgt atatttccgg tgacttcata ttcgtatccc
     2941 atcttttggt ggctgaatat tctatattcc attccactaa ttaatgattt acaaactaat
     3001 ccggaatgaa actcggattc ttgtacaacc gttttttcat taactaaatg atcaaaacca
     3061 ttacttacat taaccagaac tacatcccct tcaaatattt ctacgccatt cttatctttc
     3121 agtcctgtgg attgcataag cacatattta tctggagcca tttcagcatg agtgatcaac
     3181 cttcctgctt gcccatattt catttcttgt ccaattgttt tacctttaaa tggtgtgtac
     3241 cacgatcgaa atctcggtat catttgctgc cctccatgta ttcgtctaat atctctttat
     3301 atttctctac aaatttgaaa cgatcttgat gaagtttctt gctccaattt gtttgccgat
     3361 ccagctcacg catttgatcg aacccttttt gaatttcgtt gtaataaaat tcaatgtttg
     3421 ctgctgcctt ccaatgcctg ctacttcgca ctcctgcccc tgtttcagcc atttccaact
     3481 taactaattc cgctcgttct tttgattttt tgtctttctg aatcttcatc atgattttct
     3541 tgaggatgat atcactgtat tgtgtaatga gattcattat ttctcctcca catacctaaa
     3601 ctgtcttcct ttagaatcaa tatccatatt cttcgctcta tcccagatga tgtttttgct
     3661 cagaccagta atttcagata actgttcagc ggtacctgtt actagaattc ggtcaccgtg
     3721 ccagattgca atttttctcg gcattctccg tctggttttt tcagtccaca ttgatttacc
     3781 gagcttttgg acttctgcaa ctatttcttt gtcttcttgc caattctcag aatgtgtcag
     3841 ttcgatgatt cgtttcattg ctgctttctt atccacgctc attcctccaa tctacgaatt
     3901 tcccttctta agttctctat gtgcaaatcg attgcctttc tcgccgtttc attgaccatc
     3961 actgcctttg ttcgttccag atcgtcaatc tcacgttgaa tgcttcgaat tcgcatttga
     4021 atcacttctt ctgttgtcat gatagaccac ctctttaaaa acgctcttcc ttgaacgtat
     4081 tccgatattt tttagctaat atcaacggca cttgatattg atgacaaaac agctttgcct
     4141 tgatcttaaa gtcttttgtc tgcattcctt taacatctac gactttgaca agtttgccgt
     4201 ttttataaaa tgtgaagtcg ggaatatact cgatcttgcg atacttcttt ccgtctagtt
     4261 caaatttcgg catcagctca aatctttcct gaagttttac tttccagcca ttcgcttcag
     4321 cttgccacaa ggctagatcg taatactctg cttctgcgat agaatcaaac ttgatacctc
     4381 gatgaacagt ttttttatta cggtatttat tcatgcgata ctacctttca ctggttttat
     4441 gcgcttgtct gctgtttgct ggaatttcag cgcataacct tctgaattct taaatatcct
     4501 agaaacaatt ctttcaccgt aggcttctct tagttcagga ccagataagt ttgttgtgat
     4561 gatcgttgcc ttgttctgtc ttgcttctaa gagcgtgttt aacgtgttgt ttgtaaactg
     4621 cctactattt gataccccac tacctaattc agctccaata tcgtcaaaaa ccaccaaatc
     4681 agttgtttta atatcggcta taagcgatcc ttcaatttct tttctcagtt cagcattgtt
     4741 ataagaaaac tttatttgct ctaataactc ttgatagctt ataaaaagta ttttcttgtc
     4801 ataatttgag cgctcaagta tttcccaagc tgtcgccatt gacaagtggc tttttccgct
     4861 tcctgatttc cctgatagaa tgaaatgtgc aggatggttc agtaagacat catttacaaa
     4921 gcttttagct ctttctaaag caattttcgt ttcttggtcc actacgtgat aattctccat
     4981 tttgcattta aacaaagttt tatctgttaa taccgaacca ttttgaaaaa aactcaacgc
     5041 tcgtgctttt aagctgtcgt tatatatccg ttcggtctgt atatcctctt tcacacgtaa
     5101 cgctttataa ccacaactca tgcatgttgg tttacaacgt tctgaaccat ccttattttt
     5161 agctcgccaa ctatacaaag gttcgctaca ttctggacat ttcccgcttt gcactaatac
     5221 tcttcttatt agtttctcca tagcatttgc taggctttcc atgtgatgca tctccttttt
     5281 aaattggcaa gtcgtcatat tcactaggat tgctgtactg tagtttttga ctttgctttt
     5341 tatgattatt cttgtctgct ttgatttcga atttgagctt ctcaaatttt tctctcaatt
     5401 tcttagcact tctaatattt ccaaaccaaa attcatttgt aggtagccaa ttgatcacat
     5461 actcaatcgc ttctatagac gctttatctc tttcttccat caacctgatt gtgtctgccc
     5521 atttttcgat atctactttg ttcatttctt ttggaaaatc ttcagttaaa ttactttgca
     5581 attttttagc aaggcgtaag tgttcgtcag aatacttacc tttcttttct tctttatcta
     5641 tatctatatc tttctctatc tctatctcta actctggtgt agttttgtct ggacatttgt
     5701 ccgacacttg tcctccagtt attaaattcc gttttgcctc ttctattttc tttctgtatt
     5761 ctctttttct atctgcttca gttgaggatt ttccaatgaa actttgtata tcagacatat
     5821 aaattgctcc gttatctaat acgtcaataa gctgcaaatc acggaaaatt tgtaccgctt
     5881 tttctacgac tcctacagaa tgtcttgtaa tagttgcgag cattgtagag ttaaatggaa
     5941 tcctgtcatt aaacatcaac ttaccttcgt gttttagact tcttaaataa agtttgagaa
     6001 gaatattaga ataaatatag ccatctggca tactttctaa gagaaccatc tcgtcactat
     6061 cgaaaaaatt ctcttttagt tttaaatagt agtagcgttt gttgtcagac aatatttttt
     6121 accctcctat tctaagtttc ttaattgttt cctggtttaa cttgatccct ttgatttgat
     6181 atttattttt gaaattgatc acacctatat tgtgtttctc cgtgtgatga atcctgcaga
     6241 gtgctgcaaa tgtgtactct gaatgatcaa cttctttgcg ctttcgtctt cctagcgctt
     6301 tgtcaaagtg atcgatgtca gctcctgttt tgccacagat gcagcagact ctttttgtga
     6361 tgcatttgta gaagtaatat tcttgattcg ctggtaaaat ctcatagcct tctttgaaag
     6421 gaatatgatg ttcaaagatg aaatctaaga tgatatttgc taagacatta gcatcactca
     6481 cagttgtatt cgattcgtct ttgaggctta ttttgcgccc tgtgacgcct tcaaaacgga
     6541 agtaaaagaa ttccttccag aagtccgttg gcatgcctgt atcgatgaaa atatcgccta
     6601 tcagcgcata gatgaagttt cgttgctgca cagtaaatcg acgtggatca ataaatcgaa
     6661 cttcaataat ccgatcgcca tcatatccgt catacatcgt ctttagtcga tcaatgttca
     6721 cttcttcatt gatcgttgca cctatgtctt ttcctttgaa ctttttcaga accgctgaat
     6781 atgaatcgat caatggttta aacactcata tcacttctct tttgtttctt ctctgtactg
     6841 atcttcaatc cagttaacgc ctcgttttag aatgcccaag tctctcttgg tccatttact
     6901 gtcatcagcg gttatagaag ccgcatcagt caatgcaaca attgcttcat caattgattt
     6961 ttcgtacttg ttagcaacca gttgtaaagc atccaagaat agctttttgc ttctttgagt
     7021 agctggttca agcatcgaga catcttctgg catatcttcg ccagcaaata tatatagccc
     7081 tagcccaaac attgctagat tttttacaag acagcgcatg atcgttttgt tgatatcaaa
     7141 catagttgct gcttcaactc gcttttcgat ttttccaaca atctcttttt tcttcgtttc
     7201 gttattccac tgataatcat tgacttcgta ggtatatggc tcatctttca ttgccttgtt
     7261 tgcaccatcc atgactggta accacatgtc acgctttact ccgttgactg tgatactggt
     7321 aaaaaccata tagcctgttt tcttatcaaa gacatacggc agatatgttt ctggatcatg
     7381 atagatttca taatctactg cctcacacac tttgctaact tctgcccaag cccatgccca
     7441 cgaaagataa gtgaggttat ttcgtttttc agtaacatct ttcacattta ttttgtataa
     7501 ggtattaaaa agcgtattat cacttctatt tttcaaaggt tgttcctttt cactcatcaa
     7561 attctgcctc catttcagca atgtatttct tacctgatcc gtaataagag atatcaatca
     7621 agttatctct gtcatactct tctagcgcat caatcaagcc atcttcgatg acatagatat
     7681 attcagtttt tttggacttc ctcgataaat ggataagata gacatgatcc caaatactca
     7741 caaaatttcc caaatcgtct tgatcacatg ctagttcttc atccgtcaaa agattacgtc
     7801 tgatttttcg attgcttgtt tccttgatat tcgatttgcc ccaactagga tcagtcaaat
     7861 attgatctag agtggaaagt tcttttttca tgtggtaaca tctccttaga tgtattttct
     7921 ttgtgactca ttgctttggt cggctgagtc acttttttat ttgttgccat gctttttgct
     7981 tatcaatatg ttgttggctt aggatgtttg gtttattgtg tctccaccag cgattagcaa
     8041 ttattacgcc tatttttagc gcttcagctc tattcatcct ctaacaactc catttgctta
     8101 ataattgtca tcgtcgctgt agaaggcatc caattgttaa taaaatcgat aacatcatcg
     8161 aactgttttg ccttgatacg atttctagct actgctccag tgattcgctt aattccgcca
     8221 ttcaagtctt taaacaattc tgcttttgct ttgctattta aatgctgctg attacaaata
     8281 actctaattt tgttgcgaac catattagaa attgttcggt aatctggttc agctagaagt
     8341 tgattgttct tcaaatcgtt taaatcagac tcgatagcgt ctactctttc attcgtttct
     8401 tcgtttgcag ctagagcaag taatgccatt tctcgtttag ttgctggaat tgatggcaat
     8461 tggtctttta attctttttc catcttgttg aaggcctcaa tatattgaag tttaaaactt
     8521 aaagctttct taccagtgaa gcccattgct aatagagaga agccgtctct attcatataa
     8581 ataattcggt atgattgttt attttgagga tgaacataag tatcttccca aaataggtct
     8641 gcccaatttt cggcaacccc ctcttttagt tcatcaatag ccgtcaaaac atgtctgtgc
     8701 tttttatcga agctttctgc aacttgtaag ctcgtagtca cagcttcttt atttttcaaa
     8761 attactaatt cttgcattat ttcttctctc ctttttgata taattctggt ggggtgaaaa
     8821 catgagcaga aaaattgata atataatatt gttaacaact attttttatt tagtaatcgg
     8881 agcaactctt tccttactgt catttttgtc caacagtagt ttgattgtgt taaatcaaaa
     8941 aagcatatta acagaatttg cccacataga ttacttctct ttttttaaaa ctggactaat
     9001 atgtttattt
//
LOCUS       DAZBWR010000112         8931 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000112 DAZBWR010000000
VERSION     DAZBWR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.117"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..886
                     /gene="purR"
                     /locus_tag="ACHGT5_002383"
     CDS             68..886
                     /gene="purR"
                     /locus_tag="ACHGT5_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN7218335.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1389..2762
                     /gene="glmU"
                     /locus_tag="ACHGT5_002384"
     CDS             1389..2762
                     /gene="glmU"
                     /locus_tag="ACHGT5_002384"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN7218336.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2856..3827
                     /locus_tag="ACHGT5_002385"
     CDS             2856..3827
                     /locus_tag="ACHGT5_002385"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN7218337.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3833..3935
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4001..4570
                     /gene="thiT"
                     /locus_tag="ACHGT5_002386"
     CDS             4001..4570
                     /gene="thiT"
                     /locus_tag="ACHGT5_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN7218338.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4651..5424)
                     /locus_tag="ACHGT5_002387"
     CDS             complement(4651..5424)
                     /locus_tag="ACHGT5_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218339.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5691..7371
                     /locus_tag="ACHGT5_002388"
                     /pseudo
     CDS             5691..7371
                     /locus_tag="ACHGT5_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            7341..7559
                     /locus_tag="ACHGT5_002389"
     CDS             7341..7559
                     /locus_tag="ACHGT5_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN7218340.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7579..8469
                     /locus_tag="ACHGT5_002390"
     CDS             7579..8469
                     /locus_tag="ACHGT5_002390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN7218341.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8487..8771
                     /locus_tag="ACHGT5_002391"
     CDS             8487..8771
                     /locus_tag="ACHGT5_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218342.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3012 a         1531 c         1881 g         2507 t
ORIGIN      
        1 ctcgaatttt tgaagaaaaa cttatataat gtaaaacgat aaaatttctt aaaaggagtt
       61 ggaataggtg aaagtacgtc gcagtgaacg tctagttgac atgacacagt atttattgaa
      121 tcacccgcat gaattgattt ccttaacaag ctttgccgaa cgttatgaat ctgctaaatc
      181 ttctatcagt gaagatttgg gaatcatcaa gaaaacattt aaagaacgag ggtatggaac
      241 cttggaaaca atcccgggtg ctgcaggtgg tgtattgttc atccctgaaa tttcatatga
      301 agaggcgaaa aaatatattg aatctctagc agaacgtcta tcagaacaag accgtctgct
      361 tccaggagga tatgtttatc tctctgattt gttaggagaa cctgaactgc tgcgtcaagt
      421 tggaagaatc attgcttcta aataccttgg tgaaaaaatc gatgctgtaa tgactgtagc
      481 cacaaaaggc gtacctattg cccaagctgt gtcctattat ctgaatgcgc cattcgttat
      541 tgtacgccgg gactcaaaaa taacagaagg atcaacagta agtgtcaact atgtgtctgg
      601 atcttctgaa agaattgaaa agatggaact gtctaagaga agtttgaaac gtggttcacg
      661 tgtccttgta gtggatgatt tcatgaaagg cggcggaaca gtcaacggaa tgaagagctt
      721 gattgaagaa ttcgaatcag aacttgttgg catcactgta tttgccgaat caaaattcaa
      781 cggtcatcgc gccatcgatg attatacgtc actgctttat gtcaaagatg tagatactca
      841 aacgaaaaat attacggttg tgccaggaaa ttatttttca gaataagagg ttgtgaaaaa
      901 gctgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttccac attttttcat
      961 aaatttggct taataccgag aaactggctt ttgaacaccg tttatctaga ggttgtgaaa
     1021 tcagcgtcct gtcctgagta ttagctcaat aaacggaaaa attgctcctc atattttgcc
     1081 gtttattgag ctaataccga aggtcagctg attgaaaaac gtttaatgaa gaggttgtaa
     1141 gcctaccgtt taactctaag tcacaagaaa cgatttcaaa aaacagcttt tcatgtttta
     1201 tgagtagtgt gacttgtgac cgaggagttg gcaggagagc accggttacc aaatagatag
     1261 gcccgttatg gtctatctat ttttttatcc agaaaacgct gggataaaag aaaatcactt
     1321 gttttgttga aaaaatgggt atactgggat aaactgaaat cagcaaacta tatttaggag
     1381 tgacgcagtt ggacgcacgt tatgcaatta ttttggcggc agggaaaggt acccgcatga
     1441 agtcaaaatt atataaagtt ttacatcccg tttctggtaa acctatggta gaacatatca
     1501 ttaatcgggt cagtgaaacg aaaccagatg aagtgattac gatcgttgga cacggagcag
     1561 aacaagttaa agcacaacta ggagaacgca gtaaatatgc gttacaagca gaacaattgg
     1621 gaacaggaca tgctgttttg caagctgctt ctttcttgga aggaaaaaaa ggaacaacgt
     1681 tagttatcag tggcgataca ccattattga ctactgaaac gctgaacaat ctttttgaat
     1741 atcaccaagg aaaaaatgca agtgcaacga tcttgacggc acaagcagaa aacccaacag
     1801 gatatggacg aatcatccgt gaccatattg ggatcgtaga aaaaatcgtg gaacaaaaag
     1861 atgcaacacc agaagaagct cttgtacaag aaatcaatac aggaacttac tgttttgaca
     1921 acgaagcttt gttcgatgcg ttaagcaaag ttggaacaaa caatgctcaa ggtgaatact
     1981 atctaacaga tatcatcgaa atcttaaaag aagaaggcca tacagttgct gcttatcaaa
     2041 cggatgattt tgaagaatca atgggagtca atgatcggat cgcgttagct aaagcaaatg
     2101 agatcatgag aaagcgaatt aaccagatgc atatggtcaa tggcgtaagc tttgtcgatt
     2161 cagcaacgac atacatcgat gcaggcgtag aaatcggtcc agacacattg atcgaagcag
     2221 gtgtacaaat ccaaggaaat acagtcattg gttctgattg tgtcatcggg tcacattcaa
     2281 aaatcgtaga cagtaggatt gaggatcacg tcgtcattga aaactcagtc attgaaagca
     2341 gccatgtcaa gaaacatgca gatgtgggac cttatgcaca tttacgacca aaagctgaaa
     2401 ttggcgaaaa cgtccatatc ggaaactttg tggaagtgaa aaatgctcaa atcggcaaag
     2461 gaactaaagt cggtcacttg acttatgttg gtgatgctac gctgggagaa gaaatcaatg
     2521 tcggatgtgg tgttgtcttc gtcaactatg atggaaagaa caaacatcat acgactgtcg
     2581 gcgaccacag ctttattggt tctagcacga atatcatcgg acctgtcgag gtagctaaaa
     2641 acagttcgat tgctgccggg tcaacaatta cagacaatat tccagaatat gcgctggcaa
     2701 ttgcgagagc aagacaagtc aataaagaag gatatgcaaa aaaactccct tatctaaact
     2761 aagacttttg caaaatcact tgatttattt aaaaaatatg actactattt attaggtaga
     2821 attctagagt tgtgaaaaag aaagtggagg tccccatgtc aaaacattac tttgacccaa
     2881 gattaaaaat ttttgcgtta aattccaatc gcccattagc agagaaaatt gcagcagcag
     2941 tcggcgtcga gttaggaaaa tcatctgtta ctcaatttag tgatggagaa attcaagtaa
     3001 acattgaaga aagtattcgt ggatcgcatg tctatgttat tcaatcaacg agcagcccag
     3061 tcaatgataa cttgatggag ttgctgatca tgattgacgc actgaaacga gcaagtgcaa
     3121 aaacaatcaa cgtagtcatg ccttattatg gctatgcgcg tcaagaccgt aaagcacgtg
     3181 cacgggaacc aatcacagcg aaattagtag caaacatgat tcaaaaagct ggtgcaacaa
     3241 gaatgctgac tttggacctt catgcagttc aaatccaagg tttctttgat attccagtcg
     3301 atcatttgat gggtgcgcca cttattgcca attatttctt agaaaaagga atcaaaggcg
     3361 atgatgttgt cgttgtttca ccagaccatg gtggtgtcac acgggcacgt aaattagcgg
     3421 aatttttgaa atctccgatt gcgatcatag acaaacgccg tccaaaagca aatgttgcag
     3481 aagtaatgaa tatcattgga caagtcaaag gcaaaacttg tgtactgatc gatgatatga
     3541 tcgatacagc aggtacgatc acattggctg ccaatgcttt gaaagaagct ggtgcaacta
     3601 gtgtgtatgc ttcatgtaca catccagtat tatccggccc tgctttacaa agaatagaag
     3661 attcagctat cgagcgtcta gttgttacag actcgatcta tcttccagaa gatcgtaaaa
     3721 tcgacaaaat cgatgaagta agtgttggcg gactaatggg agatgcaatc aaacgtatcc
     3781 atgaaaacaa accagttagt ccattgtttg aaacaaaaaa taaataataa ataaactcac
     3841 aaaggggagt ccaattgggc tgagattgaa tctatttcta aacccttcgt acctgtatcg
     3901 gttatgcgag cgtaggaatt gtgaataaac agctgtttga ctattttcga aagctgcttc
     3961 ctcctttgtg acttttttat tcaaaggagg aagttttttt atgggaaaaa atagagtttg
     4021 ggtagaagga acgattgttg cagcgttagc gatggtgttg tcgttgattc cgatccagat
     4081 cggcagcagt ttttctattt cattagggca gattccattg actctttttg cgttaagaag
     4141 agggacaaag gcagggatgc tggcaggttt catttggggc atcctacatt ttccgttggg
     4201 acaagtttat tatttaagtg tcgttcaagt gttgattgag tacccgattg cctatacttt
     4261 tgcaggcgca gcagggctca tggcttcaaa tgtccaaaaa ggctatgtag acgataatac
     4321 gaaacaaatt agaaaaagca tcgtgtttgg tgcattgatt ggcgcacttt ccagatattt
     4381 ttggcatttt attgcaggag tggttttctg gggagcgtat gcactatggg ggatgaatcc
     4441 ttggctcttt tctttcgtaa tgaatggtgc cagcgggttg agtacagcta ttgtaacgat
     4501 catcgtttta ttagggatcc aacaaacgac tccttcgatt tttgtgccag ataaacgaca
     4561 ggagctgtaa ataaaaaaga agcaagaaac ttgagacaga atcatgtcct agtttcttgt
     4621 ttccttttta ttttttgata agaaatagat ctacttcaat ggcagataag aaagatcggc
     4681 atcttctacc aattgccagt gtcgatcggt gacagagtag attaaagtag cgatgatttc
     4741 ctcttctcga taccgggtgc cttttaattt ccaaatataa tcttcaatca catagattgg
     4801 gatacgatat ttaccatcga tcaaaccaac ttgatcaaaa gaaaaaatga tcccttcctg
     4861 catcaaatta cgcatttctt tcaataattc cacactgatt ttttcaacag gtgtttcttt
     4921 tttgaaaaaa gattttttta cattgatctt atctgacaag atcgaactta atttactagc
     4981 aaaaatccta taaaattcat ctaaatagcg ataataaatc cgattcagct gatcattaag
     5041 atcgaagtat acaaaattat tctgtagttt ataaaaaaac ggcgaatgaa gatgtgtttt
     5101 catatgacca aaatataata attctgaaat ttctaaaggt gtcaattctt tcagcatcgt
     5161 taaatcagta aagtcgatcc acttcaattc atcgaatcca cgcctgttac gagaagagga
     5221 aaagaaacgc tgaatatttt ctgtaccacg gattactttc agccctgtat gggattcata
     5281 ttctccagca gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat gcacgatact
     5341 gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca gcattacttg tggtatcaat
     5401 atgtacataa actgactcag tcatcatgtt cctcctctta tttcctctta atctctttta
     5461 ttttacaaca tttgcattaa aaaagcttgt ttcttttatt ttaaaattcc attacaaaat
     5521 cacgttttca ttgaaaacac tctcattttc aattaatttt tgtgattttt gtattttttt
     5581 atttaaagga ggttttatga attgttttct tatggaatct acaaagtcga atacgagact
     5641 catgaccttt ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa
     5701 aaaaagttta cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac
     5761 cccttccatt ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa
     5821 agcaatgtgc ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa
     5881 gtagaggaga tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa
     5941 aattttttcg gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt
     6001 tatttcaatc tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag
     6061 gaaccacaca attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag
     6121 gagcgcacgg tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact
     6181 ttgagattat cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag
     6241 atatgaaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa
     6301 gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa
     6361 ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta
     6421 ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg
     6481 gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc
     6541 tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac
     6601 ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca
     6661 tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt
     6721 atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa
     6781 aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac
     6841 tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt
     6901 ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt
     6961 tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg
     7021 atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac
     7081 atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca
     7141 caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata
     7201 caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat
     7261 cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct atatttatat
     7321 tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg
     7381 ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg
     7441 gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag
     7501 gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat
     7561 gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca
     7621 aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa
     7681 atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag
     7741 cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa
     7801 aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga
     7861 attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc
     7921 gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg
     7981 gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa
     8041 aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta
     8101 ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa
     8161 aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta
     8221 gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta
     8281 caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg
     8341 atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt
     8401 aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat
     8461 attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa
     8521 gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga
     8581 aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt
     8641 acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga
     8701 cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata
     8761 tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa ttggatttct
     8821 tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa
     8881 tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt a
//
LOCUS       DAZBWR010000113         8836 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000113 DAZBWR010000000
VERSION     DAZBWR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.118"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..786
                     /locus_tag="ACHGT5_002392"
                     /pseudo
     CDS             278..786
                     /locus_tag="ACHGT5_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            879..2041
                     /locus_tag="ACHGT5_002393"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="ACHGT5_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN7218343.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..3301
                     /locus_tag="ACHGT5_002394"
     CDS             2321..3301
                     /locus_tag="ACHGT5_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7218344.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3423..4841
                     /locus_tag="ACHGT5_002395"
     CDS             3423..4841
                     /locus_tag="ACHGT5_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN7218345.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4912..6381
                     /locus_tag="ACHGT5_002396"
     CDS             4912..6381
                     /locus_tag="ACHGT5_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7218346.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6391..6858
                     /locus_tag="ACHGT5_002397"
     CDS             6391..6858
                     /locus_tag="ACHGT5_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN7218347.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /gene="scrK"
                     /locus_tag="ACHGT5_002398"
     CDS             6894..7766
                     /gene="scrK"
                     /locus_tag="ACHGT5_002398"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN7218348.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7847..>8836
                     /locus_tag="ACHGT5_002399"
     CDS             7847..>8836
                     /locus_tag="ACHGT5_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN7218349.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDS"
BASE COUNT         3039 a         1319 c         1781 g         2697 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg
     3241 cgaaaaggag acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata
     3301 agttaaatcg gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac
     3361 atatgaaacg ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga
     3421 aaatggagaa attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg
     3481 tttgggtcgg agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac
     3541 gtgatactag aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga
     3601 aagattttgt tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga
     3661 gagaccagtt tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt
     3721 atacagggta taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg
     3781 ctgagagtga tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac
     3841 aagaagggta tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag
     3901 aagaatattt gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac
     3961 gtttagtgga atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg
     4021 ctccgggatt gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt
     4081 atcttgtcta ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt
     4141 tggaaggtcc ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag
     4201 gtagaacagc ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc
     4261 aagaaaatga taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc
     4321 aaaaagagga tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa
     4381 atttacatga tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc
     4441 ttgtcaaaaa tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc
     4501 ttagtgactt ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc
     4561 gcttcaattt agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc
     4621 aatatatgga taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac
     4681 taaaagtgat tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg
     4741 ctcgtgtaca tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga
     4801 ctgcaataaa taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc
     4861 aggtctgaaa atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac
     4921 aaaaaaattg ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct
     4981 acacactgca ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa
     5041 atgcttgagg aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta
     5101 attttcggaa caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct
     5161 aaggaagtat ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt
     5221 caacgagctt ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg
     5281 gcagcaatgg ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat
     5341 ggctcattcg ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt
     5401 gcaaccacat ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc
     5461 ggaaacccta ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat
     5521 cggaatgatt tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc
     5581 attccagcag ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta
     5641 gcaaaagttg agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc
     5701 ccaactgtga caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg
     5761 aattttatcg gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc
     5821 tttggtgctt ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat
     5881 gctattcaag gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct
     5941 atttggtcag tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt
     6001 aagaaaaaat ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt
     6061 attactgaac cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc
     6121 tcgtttattg gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt
     6181 ggacaaggat tgactgtatt gccaggtctg actattgcta atccagttct tccttatata
     6241 tttggtaatc tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat
     6301 aaagtgttta tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat taaatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat ctttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat
     7861 gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca
     7921 agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt
     7981 attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa
     8041 tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc
     8101 cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat
     8161 aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct
     8221 tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc
     8281 aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg
     8341 ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga
     8401 tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca
     8461 aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt
     8521 agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc
     8581 tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt
     8641 tgaatttctt aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact
     8701 ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat
     8761 caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg
     8821 ggacaaacta gacagc
//
LOCUS       DAZBWR010000114         8810 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000114 DAZBWR010000000
VERSION     DAZBWR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.119"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..213)
                     /locus_tag="ACHGT5_002400"
     CDS             complement(16..213)
                     /locus_tag="ACHGT5_002400"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN7218350.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(359..1237)
                     /locus_tag="ACHGT5_002401"
     CDS             complement(359..1237)
                     /locus_tag="ACHGT5_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN7218351.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1276..2394)
                     /locus_tag="ACHGT5_002402"
     CDS             complement(1276..2394)
                     /locus_tag="ACHGT5_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN7218352.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2391..4490)
                     /locus_tag="ACHGT5_002403"
     CDS             complement(2391..4490)
                     /locus_tag="ACHGT5_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN7218353.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4541..5362)
                     /locus_tag="ACHGT5_002404"
     CDS             complement(4541..5362)
                     /locus_tag="ACHGT5_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN7218354.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5359..6168)
                     /locus_tag="ACHGT5_002405"
     CDS             complement(5359..6168)
                     /locus_tag="ACHGT5_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN7218355.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6189..6683)
                     /locus_tag="ACHGT5_002406"
     CDS             complement(6189..6683)
                     /locus_tag="ACHGT5_002406"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN7218356.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6695..7195)
                     /locus_tag="ACHGT5_002407"
     CDS             complement(6695..7195)
                     /locus_tag="ACHGT5_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7218357.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7260..>8810)
                     /locus_tag="ACHGT5_002408"
     CDS             complement(7260..>8810)
                     /locus_tag="ACHGT5_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN7218358.1"
                     /translation="IKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNENFSSIKNEV
                     QHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSY
                     KTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLLIVVTLSDYVDESIEL
                     KGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFVKRQVNTDS
                     LVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAAD
                     KYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTKDYRELREL
                     IDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSPEMKPAEFN
                     RLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRVKLNLYMHT
                     ALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYE
                     LLGPYIQK"
BASE COUNT         2684 a         1999 c         1463 g         2664 t
ORIGIN      
        1 ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta tgaagtcaac
       61 atttcgaaaa atgaacatac attggtaagc aactataact attaccacag acataaagta
      121 acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg tatacagcgc
      181 acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta atgaaaacaa
      241 cacgatttga cttagagcct ttttatttga attcattttc gctttatcca acttcatttg
      301 atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca ttcattaatt
      361 atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt attccggcat
      421 tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca acatatccat
      481 ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt ttcattacac
      541 caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat gcacattggg
      601 caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc tggcctttta
      661 cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa tcttggtgga
      721 agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata tgccccattt
      781 ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct ccgatacctg
      841 ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca acatattcac
      901 catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa tattgcttta
      961 gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg atatgaccga
     1021 aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg atgcctgcca
     1081 attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca actggagttg
     1141 ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact gcacagacaa
     1201 atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc cttcgaatat
     1261 gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt
     1321 tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt gccgctcaaa
     1381 taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt cggtaaaaca
     1441 gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa
     1501 ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat ctctatttga
     1561 ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac gtccgaactc
     1621 atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg ttgaggttac
     1681 ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc cccaccaata
     1741 acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac cctttaatcg
     1801 atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg
     1861 aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg tataaaaaaa
     1921 tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg tttggaaagg
     1981 catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca acgagttcac
     2041 ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt caacagcagg
     2101 gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa aactgccaaa
     2161 ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc cagtaatatc
     2221 cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt tgcggcttgg
     2281 tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat tttcgggagt
     2341 gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac tcatcttttt
     2401 ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt cgtatcactt
     2461 attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt acattcaatc
     2521 gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt atcaacagga
     2581 tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat cattaggctg
     2641 acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac gacttctcct
     2701 tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc tgccaaacaa
     2761 attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct cattaaaaca
     2821 gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc tttgatttcc
     2881 tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg atataaatga
     2941 tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc ttctccatca
     3001 tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat taacccttgc
     3061 tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc atcaccgatt
     3121 ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca aattgattcc
     3181 tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc tagggcttca
     3241 aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc ggtatgatac
     3301 tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg ttcccctgac
     3361 cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac ctgcggacac
     3421 cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag taacgcactg
     3481 gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc aggaaactta
     3541 acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc aaacatggtg
     3601 aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc aggatagctg
     3661 ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga aaagctttga
     3721 taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag ctgcccaaat
     3781 agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga ttcctgaaaa
     3841 gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct tgccacttcc
     3901 tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg ctgatccgaa
     3961 tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc gtgtgtttca
     4021 ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag tatgggagca
     4081 aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg caaacagtct
     4141 tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac aatcccgttt
     4201 acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac ccaaacacct
     4261 ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat cgtcccgcaa
     4321 acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc ctttacatca
     4381 ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc gtttaactca
     4441 gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat cttttccctc
     4501 cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt ccaagtaatg
     4561 acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg acttttttat
     4621 ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta aaaatactat
     4681 cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc aaaacggtat
     4741 tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag atactagcag
     4801 cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat ttagagccta
     4861 atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata ttgaacataa
     4921 ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc gctagtcccg
     4981 cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc aatggtccca
     5041 tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt tcagaatttt
     5101 ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa
     5161 aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg tagaaatgat
     5221 taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc ggattcaccg
     5281 aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc tcatctttag
     5341 aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa gcgttgcatt
     5401 tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta gatctctttg
     5461 ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag
     5521 atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac tccaaagcat
     5581 ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg ttaatccgtt
     5641 catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc caaataaaac
     5701 ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa agaagtgcag
     5761 attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag cagccatcgg
     5821 tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg ctagtggaaa
     5881 gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg ctgtagccag
     5941 cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga cacccataaa
     6001 gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc
     6061 tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca tttcatgtcc
     6121 agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa tttttctcct
     6181 tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat cgccggtgta
     6241 ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg aatttctgac
     6301 gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg tgtcattttc
     6361 ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc aacttttacc
     6421 gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg cggatctttt
     6481 aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga tggtgcagcc
     6541 atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc tactactaac
     6601 ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat taatcgttca
     6661 tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc tcttcattta
     6721 ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta tttaccagtt
     6781 cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc agaattggca
     6841 gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt ttggtaatct
     6901 tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc tgctcgttca
     6961 cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca taagcattaa
     7021 tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga aagcctgaag
     7081 ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct ttctttctgt
     7141 tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta atcacgttaa
     7201 ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt ttagacactt
     7261 tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata agcattgact
     7321 ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata ataaaattca
     7381 tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg agccaagaat
     7441 aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg gccatttaaa
     7501 gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact catcacgatc
     7561 gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa ttttactagc
     7621 tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat atgctccaaa
     7681 ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat tgaaaaggaa
     7741 cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt ttcatcattt
     7801 tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat ggattcatcc
     7861 gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc cgacatacag
     7921 gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt gattgaatac
     7981 ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat cttcaagcca
     8041 atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg
     8101 ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag cactagacta
     8161 tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc tgacacatta
     8221 ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg attcactact
     8281 gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt cagctcgatg
     8341 gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa gttctcgtcc
     8401 ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt cgtccgaggt
     8461 agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct tggggaaatg
     8521 acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt
     8581 cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct actcattgat
     8641 aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt tatcgaagaa
     8701 aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa aatcgaatca
     8761 taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
//
LOCUS       DAZBWR010000115         8675 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000115 DAZBWR010000000
VERSION     DAZBWR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.120"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHGT5_002409"
     CDS             <1..1101
                     /locus_tag="ACHGT5_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN7218359.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHGT5_002410"
     CDS             complement(1280..1615)
                     /locus_tag="ACHGT5_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN7218360.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHGT5_002411"
     CDS             complement(1599..1862)
                     /locus_tag="ACHGT5_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN7218361.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHGT5_002412"
     CDS             complement(2322..3125)
                     /locus_tag="ACHGT5_002412"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN7218362.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHGT5_002413"
     CDS             complement(3103..4227)
                     /locus_tag="ACHGT5_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218363.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHGT5_002414"
     CDS             complement(4243..5058)
                     /locus_tag="ACHGT5_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7218364.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHGT5_002415"
     CDS             complement(5048..5920)
                     /locus_tag="ACHGT5_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN7218365.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHGT5_002416"
     CDS             complement(6005..7294)
                     /locus_tag="ACHGT5_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN7218366.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHGT5_002417"
     CDS             complement(7438..7950)
                     /locus_tag="ACHGT5_002417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218367.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHGT5_002418"
     CDS             complement(8017..8160)
                     /locus_tag="ACHGT5_002418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN7218368.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAZBWR010000116         8493 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000116 DAZBWR010000000
VERSION     DAZBWR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.121"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHGT5_002419"
     CDS             complement(<1..149)
                     /locus_tag="ACHGT5_002419"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7218369.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHGT5_002420"
     CDS             complement(593..1714)
                     /locus_tag="ACHGT5_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFN7218370.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHGT5_002421"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHGT5_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFN7218371.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHGT5_002422"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHGT5_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFN7218372.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHGT5_002423"
     CDS             complement(4304..5215)
                     /locus_tag="ACHGT5_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN7218373.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHGT5_002424"
     CDS             complement(5232..6239)
                     /locus_tag="ACHGT5_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFN7218374.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGEGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHGT5_002425"
     CDS             complement(6236..8365)
                     /locus_tag="ACHGT5_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN7218375.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHGT5_002426"
     CDS             complement(8370..8480)
                     /locus_tag="ACHGT5_002426"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218376.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1728 c         1485 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccctcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAZBWR010000117         8329 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000117 DAZBWR010000000
VERSION     DAZBWR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.122"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHGT5_002427"
     CDS             476..1339
                     /locus_tag="ACHGT5_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN7218377.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHGT5_002428"
     CDS             complement(1468..3090)
                     /locus_tag="ACHGT5_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN7218378.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHGT5_002429"
     CDS             3339..3860
                     /locus_tag="ACHGT5_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN7218379.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGT5_002430"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGT5_002430"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN7218380.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHGT5_002431"
     CDS             5941..6759
                     /locus_tag="ACHGT5_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN7218381.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHGT5_002432"
     CDS             complement(6862..7059)
                     /locus_tag="ACHGT5_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218382.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHGT5_002433"
     CDS             7228..7806
                     /locus_tag="ACHGT5_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN7218383.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHGT5_002434"
     CDS             7901..8038
                     /locus_tag="ACHGT5_002434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218384.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2513 a         1489 c         1641 g         2686 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaat
//
LOCUS       DAZBWR010000118         8312 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000118 DAZBWR010000000
VERSION     DAZBWR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.123"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHGT5_002435"
     CDS             complement(105..629)
                     /locus_tag="ACHGT5_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN7218385.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHGT5_002436"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHGT5_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN7218386.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHGT5_002437"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHGT5_002437"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN7218387.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHGT5_002438"
     CDS             complement(2351..3031)
                     /locus_tag="ACHGT5_002438"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN7218388.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHGT5_002439"
     CDS             3157..4026
                     /locus_tag="ACHGT5_002439"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7218389.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHGT5_002440"
     CDS             4052..4567
                     /locus_tag="ACHGT5_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN7218390.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHGT5_002441"
     CDS             complement(4614..5177)
                     /locus_tag="ACHGT5_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7218391.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHGT5_002442"
     CDS             complement(5232..5411)
                     /locus_tag="ACHGT5_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218392.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHGT5_002443"
     CDS             complement(5488..5997)
                     /locus_tag="ACHGT5_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218393.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHGT5_002444"
     CDS             complement(6093..6473)
                     /locus_tag="ACHGT5_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7218394.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHGT5_002445"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHGT5_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN7218395.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..>8312)
                     /locus_tag="ACHGT5_002446"
     CDS             complement(7873..>8312)
                     /locus_tag="ACHGT5_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218396.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2724 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt ga
//
LOCUS       DAZBWR010000119         8261 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000119 DAZBWR010000000
VERSION     DAZBWR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.124"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..372
                     /locus_tag="ACHGT5_002447"
     CDS             <1..372
                     /locus_tag="ACHGT5_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN7218397.1"
                     /translation="NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDR
                     ELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNP
                     NMFVNYQNDPYQFTTQVILLF"
     gene            387..1139
                     /locus_tag="ACHGT5_002448"
     CDS             387..1139
                     /locus_tag="ACHGT5_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7218398.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1842..2057)
                     /locus_tag="ACHGT5_002449"
     CDS             complement(1842..2057)
                     /locus_tag="ACHGT5_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFN7218399.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2076..2312)
                     /locus_tag="ACHGT5_002450"
     CDS             complement(2076..2312)
                     /locus_tag="ACHGT5_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFN7218400.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2570..4723
                     /locus_tag="ACHGT5_002451"
     CDS             2570..4723
                     /locus_tag="ACHGT5_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFN7218401.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4692..6107
                     /locus_tag="ACHGT5_002452"
     CDS             4692..6107
                     /locus_tag="ACHGT5_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFN7218402.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6140..7174
                     /gene="sppA"
                     /locus_tag="ACHGT5_002453"
     CDS             6140..7174
                     /gene="sppA"
                     /locus_tag="ACHGT5_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN7218403.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7176..7697
                     /locus_tag="ACHGT5_002454"
     CDS             7176..7697
                     /locus_tag="ACHGT5_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN7218404.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7694..8080
                     /locus_tag="ACHGT5_002455"
     CDS             7694..8080
                     /locus_tag="ACHGT5_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN7218405.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2939 a         1189 c         1489 g         2644 t
ORIGIN      
        1 aataagattt gttgtaaatt cgaatgtcca agtactatta ttgaagttcc tgttccaata
       61 tttgactgta ttagggtgct tggaattatt ctcgataatg caattgaagc atctatagag
      121 agtcaagata gagaactgtc tctagctatt taccgagatg ataaacagat agaaatttcg
      181 atatcgaata gttgtagaga gatactactc tctatagata ctctaatgaa aaaaggaata
      241 acaacaaaaa agggacatca agggttaggt ttatctagta tagaggaaat caataaaaag
      301 aatcccaata tgtttgtgaa ctatcaaaac gatccgtatc aatttactac tcaagttatt
      361 ttgttgtttt gagggaagga ggatttatga gttatccaat aatattatgt gaagatcaaa
      421 ttatacaatt aaatcagtta gaacgaatta ttgataattt cattcttttt cacgataaag
      481 tatttaagat agtattgaaa acacaaagtc cgttagaagt taaaaaatac ctcaaacaat
      541 tccgaccaaa gcaaggaata tatttcttag atattgattt aaatcatgaa gttaacggta
      601 tagaattagc agaagtaatc agaaaatatg atgttcaagc aaaaatcatt tttacaacta
      661 ctcatgatga gatgttaccc gtaacaataa aaagaagagt tgaaacgtta ggatttgtaa
      721 caaaagatca aacactagat gagtatcgaa acgagattgt tgagttattg ttattagcgc
      781 aagaaaggat agatgcaaca aaacaggtta cgaatcaagc atttgttttt tccataggtt
      841 ctcaaacatt tacttttgat ataaatgaca tttattttgt agaagcttca aaattaccac
      901 atagattatc attgtgtact aaagatgggc agtacgaatt ttatggaaga attagtgagc
      961 ttgaaaagaa atatcctatg ttaacaagaa ttagtcgagc gtgtttagta aatatcttta
     1021 acgttaaaga aatagatttt aaaaaacgtt cactatattt tgattcagag ttggccagaa
     1081 actttacttt agggaaagca caaaaaatta aggagaagtt gaaagaaagc actgtttagt
     1141 ggttttttct ttagggagat tgcatttttc taataaaata gattccttca ctagtgcaaa
     1201 tttttctgaa tcctattaaa gttgtaccaa tttttatagc tgttattagt tacgtgcata
     1261 acagcaatat tagttactgg aggaatattt ctatggtctg gaaccaaaaa agggccaaat
     1321 gaatagttct tttcttgttt ttattaagta ccataaaggg gaattaaaat taaatgtatg
     1381 gtggaagatg cgaaagattt ggcaaattct actaattttt agaaagtgtt tttcattttt
     1441 tagttaaaaa atttgtcctg tcaagtgcaa tcctatgatt gtacttgata ggatttgaga
     1501 taattacttt cctgttttta taaatttaac ttgcaggaac aggctttaat gcacatccga
     1561 caagtacatt accaatacct cctgtaacgg ccgaaatagg tcctcctgca gcaccaacta
     1621 cagcgctgca agtccttttg taaaaatatc tctaccatta catttccaaa gaatttttat
     1681 ctgactttaa taaaagataa aaatttaata taattataaa aactagcaca agaagcttta
     1741 gtaaaaagtt ctgtccattc caaacttttg aaaagataaa gaacatacct atgttcgtaa
     1801 tcgacacaaa ccaatcttga ggtttgatat tttttaattg tttacgcacc ggtccacatg
     1861 ctgaatccag cacctaataa agctccacct acaccacaag cagctattgc ataaggtcct
     1921 ccggctgaac aaagttttac tcctgctgtt gccccagaaa aagtagtgcc taaaactccc
     1981 acggcactcc caccttgaat atttttcaac tcttctgaag ataatataaa tgttgttttt
     2041 gtgtttttaa tatgcatact acttattctc cttttttatt tttttcgagc atcccagcca
     2101 gctgcagcac ctccagcgac agctccatat aaccctccaa caacaccgca agctggtccg
     2161 caaattgctg ttcctaaacc tgcaccacct actgcccctg tggtaagtcc acctacaact
     2221 tgccccaatt ttccccaagc attattgcct ccaccaacag tttgtgcatt tccacaatac
     2281 ttagttcctt tacattttgc atgtttaaac acgcttcttt aaataatatt ttttacggtg
     2341 ctactgtaac atacgtattt gattaacagt aaaaatcttc ctgaaactta tcaagtttat
     2401 tcttgaatag taaaatttcc aaaataaatt tgattcagga atgaaaaagt tagtgttcaa
     2461 gaactgtttt caaagttttt gtggaattat gagacaatat tttcactgta ttaaagctat
     2521 attgagaatt tttctataaa aattttttag aaggagttgt gttagataag tgataaaatc
     2581 atttcctttt gtgcagcaac aagatgaaaa agattgtggt gttgcttgcc tttcgatgat
     2641 tttaaaatat tttaaaactg atatcccgat tcataaatta cgggacctat ctgggactga
     2701 tttagagggt acttcagcat ttggattgaa aaagacattt gaaaaattgt cttttgattg
     2761 tatggctatt caagcagata gtgagatctg gaaagaaaga gatcttatct taccattaat
     2821 tgcacatata ttggttgata acagctatat gcactacgtt gtaatttata aaacgaaggg
     2881 aaacgtacta tggattgcag atcccgctaa tggaaaaaat agaaaaacga ttgaagcgtt
     2941 tcaaaaagaa tggacaggta ttcttttatt acctatgcct agagaaactt atattcctat
     3001 taaagaaaaa gtagctggat taagttcttt tttgcctata atttggaaac aaaaaggatt
     3061 agtattccac atcgttttag catcgatttt cattacgttt tttggcatag gaagctcata
     3121 ttattttcaa ggtattcttg attttttcat ccctaatcaa gcacgttcaa ctttaaatat
     3181 aatttccatt ggcttgatcg ctgtctattt atttcgggta ttgtttgaat atagtcgtag
     3241 ttatcttctt attattttag gacagcgaat gagtatggca attatgcttc agtatttcaa
     3301 acatgtgtta actttaccaa tgaatttttt tgctacgaga aaatcagggg aaattatttc
     3361 tcgttttctt gatgccagta agattattga tgctttagca agtgctacgt tatcaatatt
     3421 tttagatatt ggtatggtgt tgcttgttgg ggttacgtta gctattcaga atagtacgtt
     3481 gtttttaatc actctatgct ctttgccttt ttatgtagtt gcaatattcg ctttcgtgaa
     3541 aagctatgaa aaatcgaaca gggaagaaat ggcagccggt gcagctgtaa attctagtat
     3601 catagaaagt cttaaaggaa tagaaacaat taaagcatat agcggtgaag agaaagtata
     3661 taaccgtata gaccatgaat ttgttaaatt aatgaaaaaa tcatttcgta cggttacgct
     3721 tgataatatt caacaaggaa ttaaacattt gattcaatta attagcagtg cattaatttt
     3781 atggctaggt tcttattttg ttatggataa tagaattagt ctaggtcaac ttatcacata
     3841 caatgcttta ctagtatttt tgacagatcc tttacaaaac attattaatt tacaagtgaa
     3901 aatgcaaacg gctcaagttg caaataatcg tttaaatgaa attttcgcga ttgattcaga
     3961 aaaatcgaac cagtcttttg aatatatgct ttctgaacaa ttatttcaac aagatatcac
     4021 tgtaaaaaac gtttcattct cgtatagcat gaaggcccca acgttgaacg aaatttcatg
     4081 tacaattcct tcgcacagta aaacggctat agttggagtt agtggttctg gaaaatcaac
     4141 tcttgcaaag ttatttgttc gtttttatga agcatcagaa ggatcgatcc attatggaaa
     4201 cattaatcac ttagacattc cttttcagat attacgcgat cgagtgacat atttgcctca
     4261 agagtcattt ttctttagtg gaacaatcat tgagaatctt atattcggtt tagatagccc
     4321 accaagtttc gaacaaataa tagaagtttg tgagatagtc caactaaatg aatttattaa
     4381 tcaacagcca ttacgttacg agacattgtt ggaagaaggt ggaaataatc tttcaggagg
     4441 gcaacgacaa cgattagcct tagcacgggc tttattaaaa aatccggaca tattaatttt
     4501 agatgaagca actagtggat tagatacctt gttagaacat gcaattatga attatctcct
     4561 caatttaaaa gaaaaaacga ttatttttat tgcgcatcat ttaccaatag caaaagcatg
     4621 tgatcaaatt ttagttctcc atgaagggca tttggtagaa aaaggaacgc acgatgaact
     4681 tcgttttgcc gatggtatct atcaaaaatt atgggaaatc taggagaggg aaacaattta
     4741 tgcagaataa taattggttc gaacattcta gtatctatac taaacaacat catagcttct
     4801 accgttgggt gttataccct gtaattcttt ttttatgtat tattggattg tttttaacct
     4861 ttgctaaaaa agaggttgtt attcggacaa cggctcaatt gaaccccgaa aaaatagaga
     4921 agttacaagt gccattagaa gctaaaataa tggaaaactt cctaaaagaa aatcaatttg
     4981 ttcataagga cgatgtgttg gttagattgg attgttcttt aatagagaat gaaaaggctc
     5041 agatagaaca agaaaaccaa cgtattactc aacaaataaa gatggctcag ctatttattg
     5101 aaagtataag taaaggaaaa aatttgtttt caacggatga cagttttggc tacagtaatc
     5161 aattaaagag catgttgtca gaaaaagaat cactccgcta cgctttgaag caaagtgaat
     5221 taaatgatca aaagcaatta gaagtatacg aaaagacaaa aagacaacta gaaaaacaaa
     5281 ttgagagttc agatagtaaa ttacaagaat ggcaacaagt acaggtagct tggagtaata
     5341 atcaatcatt aaaagatttt tcaaaagaaa tgatggcaaa ctatgagaat tggcaagaac
     5401 aactaaataa tgtttctgat gatcaaaaaa atcaagtgaa actgacaatt tcagcaagca
     5461 taaatgaaca aattgagcaa ctaaaaaaag aagtagaaca gtatcagtca gaaaaagcta
     5521 aattagttaa accaactact tctgagaatg acagaattag tcaaacggaa aaaggaaagc
     5581 aagagctaga acaagcaata acaactgtaa aacaaacagt tacagagcta aatgaaaaac
     5641 aagagaagaa ccagtcagtt atcaaaagtt tggacgatca actttcaaaa ggaatactaa
     5701 aagctcctgt atctggaacg gttcatttaa atgaagaaac aaagggacaa gcagaactag
     5761 ccaaaggaac agtgcttgca gaaatttatc cgatccacga aaaatcaaaa atgaaattta
     5821 cagcactttt acctgcgaat gaaagtacct atattaaaga agggatgaag gttcatttta
     5881 aattggatca aaaaggtggg gctccgatta cgatcgacgg aactttagat gaaatttcag
     5941 aaaattctac tgccactgag aaaggtgttt tttatgcagt gaaaggaacg ttaaaaacaa
     6001 ctcaatcgtt tccgtttcgt tatggattaa ctggagagtt gtctttaatt gtaggagaaa
     6061 aaacatattg gcaacaaatt gtcgatgtgt ttctaaataa aaaataatta tttttactaa
     6121 gaatatagga ggagtttata tgaataaaag aagatggatt gctgtgggag tagcgtgcgg
     6181 attattgtta ctatccatta tcgtcgcgct aattccagga aaagataaag aggaagcatc
     6241 aaatacaacc ctaacaggaa taaataagat tttttacgga tctaacgaaa tcacagaaga
     6301 aacgttagaa gaaggcgcat caaataaaaa gattgtcaaa ctatccgtca atggagttat
     6361 tgcagataca ggtgaaagca atctttttag tagggaacaa tataaccatc agaatttttt
     6421 aacacagcta aagaaaattc aagaagacaa agcggttaag ggagtgttat tagaagtaaa
     6481 ttcacctggg ggaggaatat atgaaagtgc tgagattgct aaagaaatgg caaaaattaa
     6541 aaagctagat attcctatct atactgcatt taaaaataca gcggcaagtg gagggtacta
     6601 catttctgct ggttcagata aaatatttgc tacggaagaa acaacaactg gatcaattgg
     6661 tgtcattatt tctggtttaa attattctgg cttattagaa aaacttggcg taactgatgc
     6721 gacgtataaa agtggtgcat taaaagatat gatgtcacct caacacaaac cttcagagga
     6781 agagaataaa gttatacagg aatttgtgat gagtgcttat gatcgttttg tcaatgttgt
     6841 agcaaaagga agaaatatgg atactaatgc agtgaaagaa cttgcggatg gaagaattta
     6901 tgatggtaac caagcagtag aaaatgggtt ggtggatcaa attggttact ctgaagatgc
     6961 attagattcc ctaaaaaaag agaagaaatt aactgatgcg acggttatag aatataaaaa
     7021 tgacacaact ggctttgctt cttcttggtt aggtaataag attgctgaat ggcaagggct
     7081 aaaggcaact acatcagatc gtgtaatttc agtttttgaa aaacttggat tttctgatac
     7141 tccaaaacca atgtattact atggagggct ataaaatgaa taagaagagt ggcattgaaa
     7201 ctttgcctaa ttatgtattt gcgggatttt ggttacgatt ttttgctttt cttattgatt
     7261 taatttgtat tagcatcatc acccatgcta ctataggtct cttttacaaa atcatgggac
     7321 tggaatctga caaatccatt attacttttt atggtatagc gtcattagct atttatttaa
     7381 cctattttgt acttttgact aagctgaatt caggacaaac aattgggaaa atgatttttg
     7441 ggataagagt agtgagcttt gaggaaaaag agctaagttg gcaaactatc tttattcgtg
     7501 agtgtatatg taggtttata ttaaaaaatt ttgtatttca aattgggtat ttggtggctg
     7561 ctttcacgcc caagaaacaa catattgggg attatttttc taacacgtca gtggttgtta
     7621 ttcgttcatt agaagctatt aactatggtc aaaaaatttc taattctaaa acacttaatc
     7681 tggagagaat taaatgaata agagaaaaac agagtttatt ttgattttaa ttagtggaat
     7741 aacagggatt ttatgtagtc ttttagcatt gatgggtggt atgtttctat cttctattga
     7801 ttcaactact ttaaatacta cagaactaac cagtaaggat atagatatgt tagtcaatct
     7861 aagcgggaat gttgttttat ttggaggaat taccctagtt ttttcgataa tgattttcat
     7921 aataggtttt ttagtaagaa aaaatcaaaa aacagtggtc tatggtatta gtttactcat
     7981 tttatctatt attccatttt ttcttttaac attattgtgg gtaattcctg gagttttagg
     8041 tattattgcg ggattgatgc ttctaataag aaaatcttaa ttaggcaatg tgtagacaac
     8101 gtgaaacgat taatcaatta aaagctaaat acgttttgag aaatgataac ttgtagtcga
     8161 tggaagacat gtagataaaa aaactacgtg tcttttattt attctaattt tttcgaaaaa
     8221 aagacactct ctgattgttg tattaatgga aaaggggaaa g
//
LOCUS       DAZBWR010000120         8249 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000120 DAZBWR010000000
VERSION     DAZBWR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.125"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHGT5_002456"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHGT5_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN7218406.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHGT5_002457"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHGT5_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN7218407.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHGT5_002458"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHGT5_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN7218408.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="ACHGT5_002459"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="ACHGT5_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN7218409.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHGT5_002460"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHGT5_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN7218410.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHGT5_002461"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHGT5_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN7218411.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..>8249)
                     /locus_tag="ACHGT5_002462"
     CDS             complement(8134..>8249)
                     /locus_tag="ACHGT5_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFN7218412.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2793 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atatttttt
//
LOCUS       DAZBWR010000121         7754 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000121 DAZBWR010000000
VERSION     DAZBWR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.126"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHGT5_002463"
     CDS             complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHGT5_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN7218413.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHGT5_002464"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHGT5_002464"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN7218414.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHGT5_002465"
     CDS             complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHGT5_002465"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN7218415.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5270..5475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5738..7039
                     /locus_tag="ACHGT5_002466"
     CDS             5738..7039
                     /locus_tag="ACHGT5_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN7218416.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7108..7707)
                     /locus_tag="ACHGT5_002467"
     CDS             complement(7108..7707)
                     /locus_tag="ACHGT5_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7218417.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2299 a         1589 c         1360 g         2506 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
     5701 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
     5761 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
     5821 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa
     5881 agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta
     5941 cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac
     6001 acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc
     6061 tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca
     6121 ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc
     6181 gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa
     6241 agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt
     6301 ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag
     6361 ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga
     6421 agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga
     6481 tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg
     6541 ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga
     6601 agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg
     6661 gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg
     6721 gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc
     6781 agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag
     6841 tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg
     6901 tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt
     6961 agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga
     7021 agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt
     7081 tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag
     7141 gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat
     7201 tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac
     7261 ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt
     7321 atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa
     7381 atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc
     7441 aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa
     7501 atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt
     7561 aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc
     7621 ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc
     7681 ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata
     7741 atatatatag ttca
//
LOCUS       DAZBWR010000122         7690 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000122 DAZBWR010000000
VERSION     DAZBWR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.127"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..822)
                     /locus_tag="ACHGT5_002468"
                     /pseudo
     CDS             complement(124..822)
                     /locus_tag="ACHGT5_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            901..1077
                     /locus_tag="ACHGT5_002469"
                     /pseudo
     CDS             901..1077
                     /locus_tag="ACHGT5_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1253..1822)
                     /locus_tag="ACHGT5_002470"
     CDS             complement(1253..1822)
                     /locus_tag="ACHGT5_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN7218418.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1896..2219)
                     /locus_tag="ACHGT5_002471"
     CDS             complement(1896..2219)
                     /locus_tag="ACHGT5_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218419.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2373..3185)
                     /locus_tag="ACHGT5_002472"
     CDS             complement(2373..3185)
                     /locus_tag="ACHGT5_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218420.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3512..4387
                     /locus_tag="ACHGT5_002473"
     CDS             3512..4387
                     /locus_tag="ACHGT5_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7218421.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4401..4724
                     /locus_tag="ACHGT5_002474"
     CDS             4401..4724
                     /locus_tag="ACHGT5_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218422.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4721..5473
                     /locus_tag="ACHGT5_002475"
     CDS             4721..5473
                     /locus_tag="ACHGT5_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN7218423.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5463..7634
                     /locus_tag="ACHGT5_002476"
                     /pseudo
     CDS             5463..7634
                     /locus_tag="ACHGT5_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2289 a         1524 c         1387 g         2490 t
ORIGIN      
        1 cccctcaaag tgaactgaac cccaaaagtt gaactattta atggactgtt tccgatattc
       61 tactggagat agtccattaa atagttcaac ttttggggtt cagttcaaag agggggggat
      121 tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt gtgctccacc
      181 cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt tcttattagg
      241 gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat tataagcttg
      301 atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt ttccagtatt
      361 gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc cagctacatg
      421 caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg ttccatctgt
      481 cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat cctccgaagt
      541 agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt ctttacttac
      601 acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat tttgtgttac
      661 ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta atggaatacc
      721 atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct tttcaaaagt
      781 ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc acgaggttgt
      841 cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt atgttttgct
      901 ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg ttttttaggt
      961 cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt gaaaaaaaga
     1021 tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc aaattgagtg
     1081 cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc gaacatgttt
     1141 ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt cgttcaattc
     1201 tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt tcttatagct
     1261 catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga ctgttttgct
     1321 ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac tggtagttga
     1381 ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat ccggtctttt
     1441 tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta atcgtcacac
     1501 cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca gcattggata
     1561 cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct ttcctctcca
     1621 ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat tgttcttggg
     1681 tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt cccgcgtctc
     1741 gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa gatgtgggaa
     1801 cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt tattatagca
     1861 cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc taaccagtaa
     1921 ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata cgttttttga
     1981 tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc ataccgaggc
     2041 ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag cgttttgttc
     2101 agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa gacgattttc
     2161 acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag taaaagcatc
     2221 ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa ttgatatagt
     2281 ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac ttcttctatc
     2341 agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac ttttgtcagc
     2401 tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc aagcttttca
     2461 gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca ccgcttttca
     2521 gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt tattggcaaa
     2581 atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt tcgactgtct
     2641 cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat gaacatctca
     2701 gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa aaagcaaaac
     2761 agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata agaaaaagaa
     2821 aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc gtacttctgc
     2881 tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga ttggatcaga
     2941 aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg aacggttttc
     3001 ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag atgccgatcc
     3061 actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca gatcaaatct
     3121 ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag tttttgccat
     3181 ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat gggttcactc
     3241 ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc ctcattattt
     3301 tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt aaaaaaacat
     3361 aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat aaatagcgat
     3421 tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac aaaaaatcga
     3481 tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta tccaattgat
     3541 gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg gtgtaggtaa
     3601 aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca gagtccaatg
     3661 tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt cacaatcaaa
     3721 agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag atgagatcaa
     3781 tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga atcatgcaac
     3841 gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta cgcaaaatcc
     3901 aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt ttttattccg
     3961 actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg ataaattcga
     4021 aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag aaataaaaac
     4081 aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc agttagtgtc
     4141 agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa gcctttcctt
     4201 tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca cgcccaaaga
     4261 tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg atcgttcatt
     4321 gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc ctattcctgt
     4381 gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat tttgtgttat
     4441 ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct ttaggctggt
     4501 tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta agcagctttc
     4561 taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa aaaagtatgc
     4621 aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct cgtttacaaa
     4681 tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta caaataaacc
     4741 gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa cattaactgt
     4801 catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa gagagatctc
     4861 ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat tacagagagt
     4921 tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc gggaacatcg
     4981 cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg ctcatactca
     5041 agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc ttttttgctt
     5101 ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt ctttatggca
     5161 tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct ttcatggcaa
     5221 agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag cttttttcgc
     5281 acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga attctcctga
     5341 agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttgtcttg tttatttcgt
     5401 agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag gaggtgaatg
     5461 gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg atactaagtg
     5521 taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt cttatcctaa
     5581 ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta cgtttgctgc
     5641 tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag acattttcca
     5701 tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat ctatcccaag
     5761 gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg ctgatctatt
     5821 tatttattac attgaccgtg gattacgact attggtacgc accctttctt tctttgatgg
     5881 cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa atgattgcgg
     5941 tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc cggttgttgt
     6001 cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc ttaactttta
     6061 gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa acgagtgaac
     6121 cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag tatcaattaa
     6181 ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga cctttatatg
     6241 ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg aagatcgaaa
     6301 ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc atccaaccaa
     6361 tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac aaagaaaaca
     6421 ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat actaccttga
     6481 ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc cagcgattca
     6541 ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg ttagttttta
     6601 cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat cagcagacaa
     6661 atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc gaattgacca
     6721 aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta aaaacaagcg
     6781 gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac gattatgtgg
     6841 attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact tctatggtcg
     6901 tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca aatgggctca
     6961 gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt catgctcatt
     7021 cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca accccttcat
     7081 ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc cagcctgcta
     7141 tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag agatctgagg
     7201 tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta ttcagccttg
     7261 gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct ctgcgctgga
     7321 caagttatct tatctatctt catttttttg cttctactaa tacgattgca tatcaaaaac
     7381 ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg caggaatata
     7441 gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta actactcaat
     7501 atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa aaaacctatt
     7561 tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca aaagggagcc
     7621 gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg agaaccagct
     7681 gattgagtat
//
LOCUS       DAZBWR010000123         7676 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000123 DAZBWR010000000
VERSION     DAZBWR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.128"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACHGT5_002477"
     CDS             133..633
                     /locus_tag="ACHGT5_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN7218424.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACHGT5_002478"
     CDS             730..2577
                     /locus_tag="ACHGT5_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFN7218425.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACHGT5_002479"
     CDS             2580..3476
                     /locus_tag="ACHGT5_002479"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7218426.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACHGT5_002480"
     CDS             3526..3915
                     /locus_tag="ACHGT5_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN7218427.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..6232
                     /locus_tag="ACHGT5_002481"
                     /pseudo
     CDS             3902..6232
                     /locus_tag="ACHGT5_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6340..7527
                     /locus_tag="ACHGT5_002482"
     CDS             6340..7527
                     /locus_tag="ACHGT5_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN7218428.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         2564 a         1177 c         1650 g         2285 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg ggattacgtc
     6241 catataattg tgtaaaagtg aagcatcaaa aaagccattg atctcagtta ggagtacaat
     6301 gttttttgca gaaaacatac tcgaatagga ctgatatcaa tgactcaagt acattttact
     6361 tttgaaagcg aagaaattca agcaattata aacgaaagcg gtgcgaatga taccgcaaaa
     6421 acacttatga ctatcatgtt taatcagctg atggaagaac aacgaaatca atatattcag
     6481 gcaaatgcct atgaacgttc agaagaacgg caatcacaaa gaaatggtta ctacgatcgt
     6541 tcgtttacaa ctcgaattgg tacgttagaa cttcatgttc cacgaaccag agacggtaag
     6601 tttagtccta ctatctttga aagatatcag cgaagcgaaa aagctttgat cgctgccatg
     6661 attgaaatgg tcatttctgg tgtctccact cgcaaagtaa ctaaaacagt tgaactgcta
     6721 acggatggtg cgactgtttc taaatcattc gtttcaaatc tgatgaaaca gttggatcca
     6781 tttgtttttg agtggagaaa ccggagccta gaaggctcgg aatatccatt tttcatgtgt
     6841 gatgccctgt atatgaaagt aagggaaaat catcgtattg tctcaaaagg tgtatatatt
     6901 ggtatcggca ttgattctga tggacgacgt acgattcttg gttttgatgt tcaagacggc
     6961 gaatcggaag ataattggga taccgttttt caatcgttcg ttcaacgagg tttgttcggt
     7021 gtaaaactgg tcatttctga tgcacacaaa gggttagtta aggctgttcg caagaacttt
     7081 ttaggtgcga gctggcaaag atgccaagcc cactttttga gaaatatttt tgataagctg
     7141 ccaaagaaag tttcttctga tgtaaaagac gagttgaaga gtatctttaa agcctctgaa
     7201 cttgaattga cacgtgaaag aaaagagcac ttcttggaaa aatatggttg tgattcaaaa
     7261 ttaagtgctg cttgcgacat tttagaaaac ggctttgaag atgctattca aattttgtct
     7321 tttcctgaaa atatccgccg aaggattcgt acaaccaatg tgttggaacg gttgaacgaa
     7381 gagattcgcc ggagagaacg agtgattcgg atttttccaa atatcaattc catcactcgg
     7441 ataattggaa cacttttgat ggaaaaagac actgagtggc tggcttctcc acgaaaatat
     7501 ttagaattta attcgaataa catttagcac catgaacctg tgcgagtctc ttctttctga
     7561 ggagagacat caagagtcgc tgcgctactg ctcctgacct ctctcctcag aaagaaaggc
     7621 atggcagtta ggttcacaac gcaaaacatt gtgcttttga tgaggttgaa ctttta
//
LOCUS       DAZBWR010000124         7224 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000124 DAZBWR010000000
VERSION     DAZBWR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.129"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHGT5_002483"
     CDS             99..746
                     /locus_tag="ACHGT5_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN7218429.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHGT5_002484"
     CDS             1139..3784
                     /locus_tag="ACHGT5_002484"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN7218430.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHGT5_002485"
     CDS             4094..5407
                     /locus_tag="ACHGT5_002485"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN7218431.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHGT5_002486"
     CDS             5397..6050
                     /locus_tag="ACHGT5_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN7218432.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHGT5_002487"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHGT5_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFN7218433.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHGT5_002488"
     CDS             6947..7147
                     /locus_tag="ACHGT5_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN7218434.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2388 a         1225 c         1586 g         2025 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccac
//
LOCUS       DAZBWR010000125         7083 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000125 DAZBWR010000000
VERSION     DAZBWR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.130"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..354
                     /locus_tag="ACHGT5_002489"
     CDS             16..354
                     /locus_tag="ACHGT5_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFN7218435.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            341..520
                     /locus_tag="ACHGT5_002490"
     CDS             341..520
                     /locus_tag="ACHGT5_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218436.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(563..1033)
                     /locus_tag="ACHGT5_002491"
     CDS             complement(563..1033)
                     /locus_tag="ACHGT5_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218437.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1120..1818
                     /locus_tag="ACHGT5_002492"
     CDS             1120..1818
                     /locus_tag="ACHGT5_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN7218438.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1996..2337
                     /locus_tag="ACHGT5_002493"
     CDS             1996..2337
                     /locus_tag="ACHGT5_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218439.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2330..3001
                     /locus_tag="ACHGT5_002494"
     CDS             2330..3001
                     /locus_tag="ACHGT5_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN7218440.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3007..3699
                     /locus_tag="ACHGT5_002495"
     CDS             3007..3699
                     /locus_tag="ACHGT5_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN7218441.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            3696..4445
                     /locus_tag="ACHGT5_002496"
     CDS             3696..4445
                     /locus_tag="ACHGT5_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218442.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            4457..4726
                     /locus_tag="ACHGT5_002497"
     CDS             4457..4726
                     /locus_tag="ACHGT5_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218443.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            4732..4896
                     /locus_tag="ACHGT5_002498"
     CDS             4732..4896
                     /locus_tag="ACHGT5_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218444.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            4889..5188
                     /locus_tag="ACHGT5_002499"
     CDS             4889..5188
                     /locus_tag="ACHGT5_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218445.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            5188..5490
                     /locus_tag="ACHGT5_002500"
     CDS             5188..5490
                     /locus_tag="ACHGT5_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN7218446.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            5530..5895
                     /locus_tag="ACHGT5_002501"
     CDS             5530..5895
                     /locus_tag="ACHGT5_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218447.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            6385..6525
                     /locus_tag="ACHGT5_002502"
     CDS             6385..6525
                     /locus_tag="ACHGT5_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218448.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            6522..6704
                     /locus_tag="ACHGT5_002503"
     CDS             6522..6704
                     /locus_tag="ACHGT5_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218449.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            6717..6911
                     /locus_tag="ACHGT5_002504"
     CDS             6717..6911
                     /locus_tag="ACHGT5_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218450.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         2596 a         1082 c         1495 g         1910 t
ORIGIN      
        1 agggaggaac agctaatgca atatctagaa gcaaaaatcc caattccaga aggctatgta
       61 attatctccc aagtggatta tgaggagtta aaaaaagctg atgatactgg tagatggatg
      121 acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac ttctagagtt
      181 ttaaagaacc caagatatag aaatattatc gatatcgaga aaaacaaaaa tggatttgtc
      241 tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac acttgaattt
      301 ttagaaacaa atttttcgga aatcttaagg aggcaagcgg atgggaaaat ttaatagagc
      361 gctagtgttc agcgcaccgc taatcatcta cgctttagga ctttggggaa gcaggcaagc
      421 gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg aagctgaagc
      481 tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag aaaagagaaa
      541 ctagaatgta tattactatt actcagttta attctttcac taatttctct attgggaagt
      601 ttgtattttt gatatcaaca attttgtgga tttgggttct atttagagta aatatcattt
      661 ttgtttggtg attaaggagt ttattaaaat taattttccc tacttcaaat gcaaggtaac
      721 aagatatagc acttttagga ggaatattta gcggtacagc ttcagagtat attctattat
      781 catcgactga tcctccttta gcaattaatt tcttaaatgg agtagctgac catctgcggt
      841 ttaagttagc ggatgaaagt tctaaattaa ctaacatagc tggttctgtt gaatagttag
      901 aaatgattac ttttgtataa atcatatctg cagcgaagta agaagcatta agttcaactt
      961 ctagttgagg gcgcattcct tttattttta tccctgttaa aaacgtactc aatataaaac
     1021 ttattattga catccattga aaaattgtta agtgaaaaaa attcaaaata aacaccacca
     1081 gtttttaact aaattatacc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     1141 aaaaataaag aagctgtgac tacgagctta caagtggcag aaagcttcga gaaaaaacat
     1201 caacatgttt taagagatat tgatgcacta aaaaaagatg tgtccaattt tggacagatg
     1261 tttgtagaag gtaatgaacc agattcatat ggcagaaatc gacgagtttt cttcattagt
     1321 agagatggat ttttcttgct ggctatgggg tttacaggaa agaaagctat ctacttcaaa
     1381 caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa tactgttcct
     1441 caaacaattg aagatatgat gatctatcaa ttagaagaaa tgaaagatgt taaaaaagat
     1501 gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga aattaagcaa
     1561 aaaggaaata tgaaagttat gggagttcta gggggaaaag aaagccgagc ttatgaagaa
     1621 atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt ttcaatccca
     1681 agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat tgaaatgtgg
     1741 ttgccagaaa ctgcgatccg tatggaaatt gatcaactga acagacaaca gagacttttt
     1801 ggtgatgaca atgaatagag ctgaagcgct aagaataggg acggcaattg ctaattgctg
     1861 gtggaaatac tataaaccaa tcatcctaag ccaacaacat attgacaagc aaaaagcatg
     1921 gcaacaaata aaaaagtgac tccgccggca agcaaagagt cacaaagaaa acacatcata
     1981 aggagatttt agcatatgga aaaagaactt tccactctag atcaatattt gattgatcct
     2041 gattggggca agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg aaatcttttg
     2101 acgaatgaag aactagcttg ggatcaagat gatttaggca accatgtaac tatttgggat
     2161 catgtttatc ttatccatct atcgaagcat tcgaataaac ctgaatatat ttacgtcatc
     2221 gaagatggct tgattgatgc gctagaagag tacgacagag ataacttgat tgatatctct
     2281 tattacggac caggtaagaa atacattgct gaaatggagg cagaatttga tgagtgaaat
     2341 caaagggaca acgaactttg aaaaactttt tagtcgtaag ttaaataaaa ttctcaagaa
     2401 aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg gagattatga aaaagaatga
     2461 tccacaggca acggtaacta ttaatgagta caaacactac agggttgttt ctggaactca
     2521 tcaagacttt cttgttgagg aatataaacc ttttcttatg gatgaaactg ggacttatgt
     2581 atctgtctca gtaacggtta aaggacacac ggaaaccgaa ttatttcctg ttttagatta
     2641 tcgaaaccaa ccagttgtta aaccaaatgc aatgcaaatc aataactcat tgaagcgatg
     2701 ctttgtgaaa gcattggctc tacacggact gggattatat gtatttcaag gggaagatat
     2761 tccaacacca cctagaatcg atacaaagaa attaaacatg ctagagacga ttctagaagc
     2821 tttcaatgag cagatgggta aagatatgac caaaacctta atcgaatatg ttaatgagca
     2881 gacagataaa ttagggctct tagctgataa cgttgaaact attgaacagt taagctatga
     2941 gcaatgtgcc ttgatggagc gagcaatagc agctaagaga aaagaattag ataagaagtg
     3001 atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa gttcaaagga
     3061 aaagacataa gcgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat
     3121 gacggctacg atggtgatcg agtcattgaa attcgtttta ttgatcctag acgtttcacc
     3181 gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg
     3241 ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa
     3301 ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc
     3361 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct
     3421 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
     3481 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
     3541 gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac
     3601 aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag
     3661 aaaacaatca aaaagttaaa tattggaggg gaaaaatgac agaacatcga agttattatg
     3721 cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat
     3781 acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact
     3841 ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta
     3901 agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata
     3961 ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa
     4021 aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa gaatatataa
     4081 gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
     4141 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg
     4201 attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata
     4261 aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg
     4321 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc
     4381 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
     4441 tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta
     4501 taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg
     4561 aattgaagga attaagaatg atcgttcgag ttggagtaaa ctcagatact atttcgcact
     4621 ctatatcgat ggaacgcaat taagaaattt agcatatacc aaattactat tcattgattg
     4681 tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaaacaa gatggaccta
     4741 aagacattta cagcacagat cgaattaatg catcaagaag ctttaagaca aagtgtgtcg
     4801 tacgaagaca agtggctcaa cacatttcat ggcggacgtg agagcgcact tgatcaagta
     4861 ctcaaattac tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat
     4921 cgaattgact tattcagaag attggcagga agataaaaaa atagttgcag aagtccaaaa
     4981 gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat
     5041 ctggcatggt gatcgaattc tagtaacggg taccgctaaa cagttatctg aaattactgg
     5101 tctgagcaaa aatattattt gggatagagc taggagctta tggattgatt caaaaggaag
     5161 acagtttagg tatgtggagg agaaataatg gacgaactaa tcgcgaaagt agagcagtgg
     5221 gcaaaagata agggattgga tcaagcggat ttcagcaagc aaatgttaaa aacggtcgaa
     5281 gaaatcgggg aagttgccgc ttctctagct agaaaagatg aacatggttt aagagacgga
     5341 attggcgatg tagtagtaac cttgatcatc ttggcaatgc aaaatgatat ggatttatac
     5401 gagtgcttgg attttgctta tgacgaaatc aaaggtcgga ctggaaagat ggtcaatggt
     5461 gttttcgtta agtccagtga cctgaaataa ttccattaat ggaagaaata actgaatagg
     5521 aggatagaca tgattgatgc aaaacaagtg caaaaacaga aagatggaat gttgatgttt
     5581 gaagcatatg ttctaccatt tcttaatcaa tttgaagttt tggaatgctc tgccagcgga
     5641 gaagaattgg agtatgttgt aattcgtgaa accaaagaaa acgttcagaa gctaaacgaa
     5701 tttctttgca ctattaactg ttgggatatg atagctccag gatttttatg tccagctatg
     5761 ggagaatttt tggaatattg tcgtcttgaa gatgcaggca ctttggattt agcttatctg
     5821 gtatacaact acctaaacat caacacagat catttgtggt ttggcactgc cgaaagaaaa
     5881 tgggtagtcc gctaaccacc aatatcaatg aaaaaaggcg gtttcctgaa agtggaattg
     5941 ccgccattgc tgtaaggttc tttacgcgac tcaaggtcac agaaatataa atgcaaatct
     6001 cttttgaagt gatccaaagc tctcggaaaa tggaagtttt ttgtttcaaa gaataaatga
     6061 gatttgcgtc aagaacctga gagttatttc caaaaaagct tccaaagaac cgaatgagct
     6121 aaggacatca tgtgtaaaca cataatatag ctctcctttc acggctaatc ataacatgaa
     6181 aaatattcga tgtcagtagg aaaaaaggtg aattatgaaa agaatattaa attatcccgg
     6241 cagtaacaga ttctgcaatt aaactccgaa gctgatgtat cagtatcaac tgtagaatat
     6301 tatgagcgtg gagaaagtca agcagtcaca aatgtaaaaa tcgataacgt caacggtctt
     6361 atctgtttag atgaaggcga tagtatgaat gaaattgctg cagaccatga attatcagaa
     6421 actgctttaa ttgatcaaat cgaattagct agacaatact ttgttccata tgcagatacc
     6481 tggtccaagc ataagaatga catcattttt ccagaggaac aatgagagca actgatccag
     6541 taattatcct tgaggaagcc aaatttattt ggactcacga agagatagag caagcacgct
     6601 tgctcttttc tcaaggagtt aagccgagca aagtagctga aataatgggt caaaagattc
     6661 ttgatgtcgg attgcttttg ctccatctag cagaaaaaat ttgatttgag gtgaaaatga
     6721 tgattcaact tgcaggcatc caaacaggga aaatttattt ttctggagaa agcaaaagcg
     6781 aagccaacca atggctattg aaaacttaca cgaacaataa gaagctaagg aaaaaatatc
     6841 ctgatttgtt gctaaaggat gatcagatta tgccggaacc gatgattttg acccgtaagg
     6901 agggaagttg aaactaaaaa accagccgtg tgggctggag ttgctatttc tttttaataa
     6961 tgagtttttc taaatttaat tcattctcac gagtttgcgt ccaataatca gaataatatt
     7021 ttaattgttt aagagtagga atgtcgtctt cggtaaaatt agaaaataat tctggagaat
     7081 tat
//
LOCUS       DAZBWR010000126         6888 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000126 DAZBWR010000000
VERSION     DAZBWR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.131"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..765)
                     /locus_tag="ACHGT5_002505"
     CDS             complement(13..765)
                     /locus_tag="ACHGT5_002505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7218451.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="ACHGT5_002506"
     CDS             complement(1129..3795)
                     /locus_tag="ACHGT5_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN7218452.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="ACHGT5_002507"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="ACHGT5_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN7218453.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACHGT5_002508"
     CDS             complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACHGT5_002508"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN7218454.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         1965 a         1464 c         1192 g         2267 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaacaa acggtgttct cctgccaact cctcggtcac
     5701 aagtcacaat attcaacaaa catgagaagc tgttttttga acttgttcct tgtgactcgg
     5761 agctggacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
     5821 taataagtcc aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac
     5881 ttgatgcagg gcaacttttt cacaacttct ttcttttact ctaattcacc agctgcccaa
     5941 tgattgatgg ggccatattt gtgccctaca tttatttctt cacgaattgc acggtttaca
     6001 tagtcttttg ccaatcggat cgcttgttca actgtttttc ctttcgctaa ctcagctgca
     6061 atacatgcag atagcgaatc gccagttccg ttgatccggt cggtaggata aaatggtgca
     6121 tttaaccaaa atgattttcc tgattcgaac aatacgtagt cattgactgt ttcttgctgc
     6181 gcatttttag catgatgtcc tttgatcata acgttttttg cacccatatc ttgcagttct
     6241 tttgctgctt gcacaaaatc ttcttcagct gctaatgtcc gtccagtcag cttttctgct
     6301 tcataaaaat taggtgtcag tacttcggcc aaagggacaa gcttttcttt caagctttgc
     6361 aaagcagatt cttctagtaa aagatttcca tgtttcgtta cgattacggg atccacgact
     6421 aatggtccaa aatcatattt ttggtaattt tttacaactg tctcaatcaa ctttgaatct
     6481 gctagcatac ctgttttagc tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct
     6541 ttgtcgataa aatctgttgg caatgtcaca cttgctccga ttccgtaaga attacctgca
     6601 acacatgcgg tcatgacaga aacaccgtaa acatttcttg taaagaatgt atgcatatct
     6661 gcttgcatcc ctgcgcttcc atcgctatct gatcctgcga tcgtcattgc ttgtacatac
     6721 tgatcttcca tcaaatcgat tcctccattt ttataaaaag atttttcata atactgttac
     6781 tgtttctatc atatgttttt caaagtatcc ctcagtaaat ataccactac cttttcttgg
     6841 taatcaaatt tatttgaaaa aacatttgca ataaatcaga aaatagtg
//
LOCUS       DAZBWR010000127         6719 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000127 DAZBWR010000000
VERSION     DAZBWR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.132"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHGT5_002509"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHGT5_002509"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFN7218455.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHGT5_002510"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHGT5_002510"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFN7218456.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHGT5_002511"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHGT5_002511"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFN7218457.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACHGT5_002512"
     CDS             complement(4028..5044)
                     /locus_tag="ACHGT5_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7218458.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACHGT5_002513"
     CDS             complement(5146..5298)
                     /locus_tag="ACHGT5_002513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218459.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="ACHGT5_002514"
     CDS             complement(5315..6634)
                     /locus_tag="ACHGT5_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFN7218460.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1771 a         1636 c         1158 g         2154 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatact
//
LOCUS       DAZBWR010000128         6634 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000128 DAZBWR010000000
VERSION     DAZBWR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.133"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1219)
                     /locus_tag="ACHGT5_002515"
     CDS             complement(89..1219)
                     /locus_tag="ACHGT5_002515"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFN7218461.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1279..1506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1868..3070)
                     /locus_tag="ACHGT5_002516"
     CDS             complement(1868..3070)
                     /locus_tag="ACHGT5_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7218462.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3130..3333)
                     /locus_tag="ACHGT5_002517"
     CDS             complement(3130..3333)
                     /locus_tag="ACHGT5_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN7218463.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3475..4539)
                     /locus_tag="ACHGT5_002518"
     CDS             complement(3475..4539)
                     /locus_tag="ACHGT5_002518"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN7218464.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4600..4707)
                     /locus_tag="ACHGT5_002519"
     CDS             complement(4600..4707)
                     /locus_tag="ACHGT5_002519"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218465.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4870..5682
                     /locus_tag="ACHGT5_002520"
     CDS             4870..5682
                     /locus_tag="ACHGT5_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218466.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6056..6247)
                     /locus_tag="ACHGT5_002521"
     CDS             complement(6056..6247)
                     /locus_tag="ACHGT5_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218467.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         2013 a         1335 c         1035 g         2251 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag ggaataagaa
     1561 caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat aaagcttttc
     1621 taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa atgatcatac
     1681 ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa aactaagtcc
     1741 tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac atcttgaaca
     1801 ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg gtagaaccgt
     1861 ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc tcttgaacat
     1921 tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg taagttccta
     1981 aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt tgaatattct
     2041 gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc ggtttgacac
     2101 ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc gtaaatgttc
     2161 cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca taccactgtc
     2221 tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat ttagttttgg
     2281 gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca atcgttatcg
     2341 tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca atccgaatgc
     2401 cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc ttatcatcct
     2461 tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact ttcttattct
     2521 cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc tctttaacag
     2581 cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat tttgataacg
     2641 tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt tcaaataacc
     2701 aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa aactgcatac
     2761 gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt ctctcgtacc
     2821 attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta gaacacagtt
     2881 gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa gcaattttgg
     2941 catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca ttttcatcta
     3001 tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa atatttttaa
     3061 tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat agtaccgcgc
     3121 ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc gggatggcac
     3181 cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa gatatgcctt
     3241 gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta tcaaaattgc
     3301 acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt ctttactcct
     3361 tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt tatactcgtc
     3421 ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc ttttttagta
     3481 gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa cctcctcctg
     3541 ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg gattcatttc
     3601 tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga cctgtgcaat
     3661 aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct caaaactttg
     3721 aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg ctgcccattt
     3781 ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa ttgctccatt
     3841 aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct ttgccaagct
     3901 ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg cctcttctaa
     3961 aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa aacgacactg
     4021 actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta tgatatccgt
     4081 accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta ttaccttatg
     4141 cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat caatccgtgt
     4201 aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg tttttcgtgt
     4261 ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca cagttccttt
     4321 aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt attggccaat
     4381 atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt caataatctc
     4441 ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat caatcaaaac
     4501 acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca atttttcttc
     4561 acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa tccccacttt
     4621 ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa tggcttcacc
     4681 gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt acttgtcaaa
     4741 gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta ttttaagtca
     4801 atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt atcctgagga
     4861 ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca gattcgccaa
     4921 gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc gcgaagtaca
     4981 gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca ccaaatcgca
     5041 gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa tcaatacatt
     5101 ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg tctcatagta
     5161 gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat gattgaattt
     5221 tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca atgtatcgat
     5281 gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca gctctatttg
     5341 caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc caactttagt
     5401 gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt tgggaaacat
     5461 ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca attgaccagc
     5521 gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca gtacacagag
     5581 gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat ggaagtcgag
     5641 aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa aattgataag
     5701 cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca cagtttcctg
     5761 ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg ctacgccttc
     5821 ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg acctggagtt
     5881 tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc ataatcagcg
     5941 cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct ggttagttaa
     6001 tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc aacctttaaa
     6061 aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca ttctcaaatt
     6121 ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat ttccattttt
     6181 gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg aaaaattttt
     6241 gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct caaattattt
     6301 ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt cctaataaag
     6361 aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt tcttattttt
     6421 cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc aatcatttca
     6481 aggtaatata actacctgag tttattatcg attaaaatgc taactacatt tgcaagagaa
     6541 gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat aggaataaaa
     6601 tatttaaaat gtatagaaac tttctttttt tttc
//
LOCUS       DAZBWR010000129         6427 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000129 DAZBWR010000000
VERSION     DAZBWR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.134"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..626)
                     /locus_tag="ACHGT5_002522"
     CDS             complement(<1..626)
                     /locus_tag="ACHGT5_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN7218468.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            937..1929
                     /locus_tag="ACHGT5_002523"
     CDS             937..1929
                     /locus_tag="ACHGT5_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7218469.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1989..3410
                     /locus_tag="ACHGT5_002524"
     CDS             1989..3410
                     /locus_tag="ACHGT5_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN7218470.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            3403..4902
                     /locus_tag="ACHGT5_002525"
     CDS             3403..4902
                     /locus_tag="ACHGT5_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN7218471.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4941..6335
                     /gene="uxaC"
                     /locus_tag="ACHGT5_002526"
     CDS             4941..6335
                     /gene="uxaC"
                     /locus_tag="ACHGT5_002526"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN7218472.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         2084 a         1201 c         1311 g         1831 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      721 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccctaa ttaaatgagc
      781 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
      841 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
      901 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatatcgct
      961 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
     1021 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
     1081 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
     1141 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
     1201 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
     1261 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
     1321 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
     1381 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
     1441 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
     1501 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
     1561 ctagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
     1621 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
     1681 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
     1741 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
     1801 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
     1861 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
     1921 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
     1981 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
     2041 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
     2101 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
     2161 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
     2221 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
     2281 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
     2341 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
     2401 cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
     2461 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
     2521 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
     2581 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
     2641 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
     2701 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
     2761 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
     2821 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
     2881 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
     2941 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
     3001 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
     3061 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
     3121 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
     3181 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
     3241 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
     3301 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
     3361 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
     3421 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
     3481 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
     3541 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
     3601 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
     3661 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
     3721 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
     3781 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
     3841 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
     3901 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
     3961 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
     4021 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
     4081 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
     4141 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
     4201 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
     4261 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
     4321 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
     4381 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
     4441 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
     4501 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
     4561 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
     4621 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
     4681 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
     4741 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
     4801 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
     4861 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
     4921 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
     4981 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
     5041 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
     5101 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
     5161 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
     5221 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
     5281 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
     5341 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
     5401 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
     5461 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
     5521 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
     5581 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
     5641 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
     5701 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcttacag
     5761 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
     5821 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
     5881 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
     5941 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
     6001 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
     6061 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
     6121 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
     6181 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
     6241 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
     6301 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
     6361 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
     6421 ccaaatt
//
LOCUS       DAZBWR010000130         6349 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000130 DAZBWR010000000
VERSION     DAZBWR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.135"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1641
                     /locus_tag="ACHGT5_002527"
     CDS             190..1641
                     /locus_tag="ACHGT5_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN7218473.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1990..2661
                     /locus_tag="ACHGT5_002528"
     CDS             1990..2661
                     /locus_tag="ACHGT5_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN7218474.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            3209..4648
                     /locus_tag="ACHGT5_002529"
                     /pseudo
     CDS             3209..4648
                     /locus_tag="ACHGT5_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4951..5160
                     /locus_tag="ACHGT5_002530"
                     /pseudo
     CDS             4951..5160
                     /locus_tag="ACHGT5_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5419..5634
                     /locus_tag="ACHGT5_002531"
     CDS             5419..5634
                     /locus_tag="ACHGT5_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218475.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5822..6109)
                     /locus_tag="ACHGT5_002532"
                     /pseudo
     CDS             complement(5822..6109)
                     /locus_tag="ACHGT5_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2123 a          990 c         1257 g         1979 t
ORIGIN      
        1 tatttatttc atcctagttt tagccaaccc tataatttta ttttaaaggc agtaacagga
       61 ttaaacattg aaacataatc gttactaaat gaaatcaaga aatatactgt attactcaat
      121 tcattgaaaa tatttatcat aatttttaat agaaaacact attaaagaga aaaaagagag
      181 gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc
      241 tttatagcaa cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa
      301 gaaagaaccg agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt
      361 ttgacaactt tatcaagtga aagtagtacg acttcaaatg ctgagtcttc aacaacttca
      421 tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt
      481 agtacgactt caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca
      541 agtgccgaat cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca
      601 agaccaaacc atatagagtc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa
      661 aatttttcga caccacagca aatagaatca acagaatctt tccaatcaca accaatacta
      721 tctggtaatg aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa
      781 attggtgagt ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt
      841 gcacaagcga tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac
      901 aatttatttg ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa
      961 gatttgggta atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat
     1021 gaggaaagtt ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat
     1081 ttttatgcag gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt
     1141 acaggacggt atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc
     1201 tatgatttaa ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac
     1261 caagtgccta ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt
     1321 caccgaggac ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt
     1381 aaggttgtta aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa
     1441 gatggatcaa ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat
     1501 gttttgcaag gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac
     1561 cttcatttgg aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa
     1621 accttattat tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt
     1681 ggattcttag acctttgatc aggatattga gactggaatt gatatctcga ttacgatgag
     1741 tttgacaaat aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct
     1801 catattttga gcaggtttta ctagatgaaa acaatttatc tatcttgagg atttcgtgta
     1861 cgtatcgttc agtcttgtat tataaattag tcataaaatc agactaagaa aaattgaaaa
     1921 tatttttttc tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg
     1981 gtaggtgcag tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat
     2041 ggatataaaa aatatcgtta cctatgtgac tgtgggaatg aaacgattgt atatcgatca
     2101 aacttactat caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa
     2161 tataaaaatt tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg
     2221 cgaagtgaag gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta
     2281 tcggcaaagc gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag
     2341 catcacattg aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac
     2401 tgttcatgga aaacaaaatg ggtttgtcaa tgtgagtgtg gcagtatgtg tgagataagt
     2461 tatagtaact tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca
     2521 aggacatgtg ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat
     2581 acaagtggtg ttaagggagt ttatcgttct aaagagaaat ggattgcgta tataacttta
     2641 aaaaaaagag gtactattta ggatcgtatg attctattaa gagtgcagct caagttagaa
     2701 aagatgctga aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat
     2761 tattgtggat aataattaac agtgatatat aaacatctat aatatattat tcatactatt
     2821 gacatttagt ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg
     2881 gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat
     2941 tttcttacgt ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt
     3001 tagaaattgc tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa
     3061 taaagcatct caaacttaca tggttacatg agaataacaa atcgaaatta ctcaagcttc
     3121 ctatggctag agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct
     3181 acacgatcat tagaaaggaa atcctattat gcatagttat caagaagatg caaaagtcca
     3241 aaaaaatcgc tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga
     3301 tggcagtatt gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa
     3361 tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt
     3421 tggtaaaatt ggcgatagct gtgggaaaat caaagtattt cggattggta cgctgatctt
     3481 tgtcttgggc tcgctgttgt gtgggttcaa tcattcgcta ggtatttaat tgttcgcacg
     3541 ggtggtacaa gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga
     3601 agtctttccc atacatgaac gtggtcgggc attgggagca atcggtgcgc ttgtttcatt
     3661 aggctcgatt gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta
     3721 cattttttgg atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt
     3781 accaaaagat atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc
     3841 ggctacgatt atcagccttt ttggaacaat ctttttgggg caagaaagtg gctttttcca
     3901 tccagtcccc cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt
     3961 tgaaaagaaa gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac
     4021 catgagcttg attacagcct tgttgatttt ttcttccaac ttctttgtta atgtagtgat
     4081 tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc tatgccggac tgttgatgat
     4141 ggtctttccc ttgatgatgg tcgtcggggc gcctttaagc ggatacctga ccgaccgggt
     4201 tggcccggag atttttgtat tagtaggctt gggtttattg tcggtaaccc aagtcatgta
     4261 tatgtttttg tcagtggact ctcctttgtg gctgtacatt gtcgctactg ccatcatggg
     4321 tgccggcaat gccttgttcc agtccccaaa taatacgatg gttatgagtt ctgtcactat
     4381 cgaaaatttg ggactggcag gctccatgaa ttcctttgcc cgaaatttgg ggatggtcat
     4441 cgggatttcg ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccaacg
     4501 agtaactaac tatatcgcag atcggccgga tgtcttttta tatggaatga agattacctt
     4561 tttaggcagc tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa
     4621 acaaggaggt attcagaaaa atgtctgaaa aaccattgtt gaagaatttt tggaggcctg
     4681 cgtatgggtg agtgggattt ccatggatga tggaagatga agtgaacttc tgaaaaccga
     4741 aaaactcaag tattatcttc aaaatactag tactcaatta gatgctcgat ttgattacaa
     4801 gaagactgcg aaagccactg gcaaccgcaa gctaggtatc caccgatgga ctacgtttta
     4861 gaggcaactg gttatgaata cggagctaac gctctcatct gcataagaat gcaacaccca
     4921 gattatgtaa agattttcga tacttctgtc aaaaaaatca tcaagataga taaatgattt
     4981 ccgtctagtc aaattggttc agaatgtgga cacacagatg gtaagaagtc attcacaatt
     5041 cgagaatgga cttgtcctat ttgccgtaca catcatgatc gaaacgtcaa cgttagtatc
     5101 aatattttga tcaagggcta tgtatacaag cattgcctta gaaaacatca agaaccttag
     5161 gaacaacggg gatagattgg ttaataagag atatcgctgt tagtaaagac ataggcttgc
     5221 aagtatactc ttttatcaag aattccgtgg ctttagtcat gataggttta atactatgaa
     5281 aattttcttt agcatttttg atttaggttc cttttctaat tataataaaa tgaccactac
     5341 agtaacctac tgaaatatat aaacagccac tgtagtacca ttttcaaagt agtctatgag
     5401 aggagaatct tcattcttat gaacgctgga attgcggata tgaatttaat aaaaaaaacc
     5461 cttaatgatt ttacttccaa ttcaatatca aaaggtactg gtatcaattt aagcacaata
     5521 aaaaagctga aaagtggtga aagatcagtg gaaaagctga atcttttaga tgctataaaa
     5581 ataacggaat ttgctatgaa aaatggaaaa gctgagattg aaatatggag ataattatcg
     5641 tctagtgaac tacttgccac ttagctgaat ttaacgatcc ttagtgcttg aagtgggaga
     5701 ttcttgggaa tagagcatac ttgcaagctt atgtctttat taacagcgat atctcttatt
     5761 aaccaagcta tccccgtagt tctaaggttt ttacttttat ctaagctaat gatcgtattc
     5821 aaaggaaatt tttgaccctt agaaagtctc attttatcag acatgagctt aggcattgct
     5881 gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt agtctttatc
     5941 acgatttcac tcaactaagg taatgtgatg tgattctgat attctttcaa agatagtttt
     6001 ttgcataagc taacattaca tcatcaatga gatgtttaca atgtttcact actaaaataa
     6061 ggtggtaaca aagaagaaat aatgaatgat tatttgtatc taattccatg gtatatcaac
     6121 catttcttat atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt
     6181 gtcggttttc gagttaccga acggcattca tcacccacct atagaggatg ggcgacttct
     6241 gcctgaaata agttaaacta cttataactt ctcataaatt tagattgagt cattattatt
     6301 taacatcaat gattttccag tttccatttt tttgtaatat taatttgta
//
LOCUS       DAZBWR010000131         6180 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000131 DAZBWR010000000
VERSION     DAZBWR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.136"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHGT5_002533"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHGT5_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN7218476.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACHGT5_002534"
     CDS             499..861
                     /locus_tag="ACHGT5_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN7218477.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACHGT5_002535"
     CDS             913..2595
                     /locus_tag="ACHGT5_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN7218478.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACHGT5_002536"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACHGT5_002536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN7218479.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACHGT5_002537"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACHGT5_002537"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN7218480.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACHGT5_002538"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACHGT5_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN7218481.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2055 a         1078 c         1418 g         1629 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
//
LOCUS       DAZBWR010000132         6174 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000132 DAZBWR010000000
VERSION     DAZBWR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.137"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHGT5_002539"
     CDS             725..811
                     /locus_tag="ACHGT5_002539"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN7218482.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACHGT5_002540"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACHGT5_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1629..1907)
                     /locus_tag="ACHGT5_002541"
     CDS             complement(1629..1907)
                     /locus_tag="ACHGT5_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218483.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1867..2781)
                     /locus_tag="ACHGT5_002542"
     CDS             complement(1867..2781)
                     /locus_tag="ACHGT5_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN7218484.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACHGT5_002543"
     CDS             complement(2763..3143)
                     /locus_tag="ACHGT5_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN7218485.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACHGT5_002544"
     CDS             complement(3593..3880)
                     /locus_tag="ACHGT5_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN7218486.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACHGT5_002545"
     CDS             complement(3900..4124)
                     /locus_tag="ACHGT5_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN7218487.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACHGT5_002546"
     CDS             complement(4579..4749)
                     /locus_tag="ACHGT5_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218488.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACHGT5_002547"
     CDS             complement(4764..5297)
                     /locus_tag="ACHGT5_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN7218489.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACHGT5_002548"
     CDS             complement(5318..>6174)
                     /locus_tag="ACHGT5_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN7218490.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAZBWR010000133         6154 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000133 DAZBWR010000000
VERSION     DAZBWR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.138"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACHGT5_002549"
     CDS             complement(137..1099)
                     /locus_tag="ACHGT5_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN7218491.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACHGT5_002550"
     CDS             complement(1101..2540)
                     /locus_tag="ACHGT5_002550"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN7218492.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACHGT5_002551"
     CDS             2725..4671
                     /locus_tag="ACHGT5_002551"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN7218493.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4935..5807
                     /gene="scrK"
                     /locus_tag="ACHGT5_002552"
     CDS             4935..5807
                     /gene="scrK"
                     /locus_tag="ACHGT5_002552"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN7218494.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1958 a         1051 c         1093 g         2052 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4801 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4861 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4921 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     4981 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     5041 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     5101 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     5161 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     5221 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     5281 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     5341 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     5401 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     5461 actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac
     5521 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     5581 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     5641 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     5701 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     5761 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     5821 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     5881 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     5941 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     6001 tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat aggttctgtt
     6061 gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata gcaggaggaa
     6121 tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAZBWR010000134         6146 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000134 DAZBWR010000000
VERSION     DAZBWR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.139"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..780)
                     /locus_tag="ACHGT5_002553"
     CDS             complement(406..780)
                     /locus_tag="ACHGT5_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN7218495.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="ACHGT5_002554"
     CDS             complement(803..2203)
                     /locus_tag="ACHGT5_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFN7218496.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="ACHGT5_002555"
     CDS             complement(2207..2617)
                     /locus_tag="ACHGT5_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN7218497.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="ACHGT5_002556"
     CDS             complement(2635..3126)
                     /locus_tag="ACHGT5_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN7218498.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="ACHGT5_002557"
     CDS             complement(3136..3927)
                     /locus_tag="ACHGT5_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN7218499.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="ACHGT5_002558"
     CDS             complement(3920..4693)
                     /locus_tag="ACHGT5_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN7218500.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>6146)
                     /locus_tag="ACHGT5_002559"
     CDS             complement(4849..>6146)
                     /locus_tag="ACHGT5_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN7218501.1"
                     /translation="LCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQK
                     LLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETN
                     KLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESN
                     VIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNVIV
                     SCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTTEF
                     YIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSK
                     KVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLF
                     DTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1979 a         1225 c          790 g         2152 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag
     5281 attttctaaa tttaaacaat cgattccttg aatgtaaaat tcagtggttg aaagaacaca
     5341 aattgtattc ttaaaaatca tagtatcttt ttcctttgct attttcttta atgcgttaaa
     5401 atcaaaaatc aaaatctcca cttgattatc gagataccgt tttaaaatat ccttaacttt
     5461 ttcagaaatc ccctcaccag agagacaaga tactattaca tttttctttt gtgataatcc
     5521 tttataatac tgaactttaa cttgaaacga atcaaaatcc atctggtaaa aatgactaat
     5581 tggctttttt tggcaaactt gaatcccaca ttcaatcgct aaagctgtag aaacattgtt
     5641 taaaattacc aaatcaccta ttacattgga ttcaattttt tcatacattt gttccaatga
     5701 tcccatatca actagcagaa tgagtccatt cgtcgtatct atacttttac aaaaattgtt
     5761 gattaggtta attaagtcac tagtctcacc ttcaattggc atatcgaaag cctcataaac
     5821 atatgtattc agcaacttat ttgtttccct tgcgatcgca gaagctgtta ttttaccgtg
     5881 cataactatc agacaatggg tttctggtat tttcacatag aacttcttta caagaaaaag
     5941 tatccatttc aacaaaagtt cttgtttact ttcttcgact tctaaactac tccaaatctc
     6001 atttgccaat aatttttgtt tatcattttt catacgaaat tttctgaact tagacagcaa
     6061 aaaatttctt gatgtttctt caattacatc aataaacatt ataaatagac tgatatccgc
     6121 caagaattca tctaataaac atagac
//
LOCUS       DAZBWR010000135         6122 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000135 DAZBWR010000000
VERSION     DAZBWR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.140"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..600)
                     /gene="yajC"
                     /locus_tag="ACHGT5_002560"
     CDS             complement(220..600)
                     /gene="yajC"
                     /locus_tag="ACHGT5_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN7218502.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(697..1842)
                     /gene="tgt"
                     /locus_tag="ACHGT5_002561"
     CDS             complement(697..1842)
                     /gene="tgt"
                     /locus_tag="ACHGT5_002561"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN7218503.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2060..2758
                     /locus_tag="ACHGT5_002562"
     CDS             2060..2758
                     /locus_tag="ACHGT5_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN7218504.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2926..5565)
                     /locus_tag="ACHGT5_002563"
     CDS             complement(2926..5565)
                     /locus_tag="ACHGT5_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN7218505.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1727 a         1327 c         1116 g         1952 t
ORIGIN      
        1 tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac aggtgtttgt
       61 atagaaaaat atataaagtt atttattatt atttatatga cacacccgat tattcaatat
      121 tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca
      181 tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc
      241 tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc
      301 tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt
      361 gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt
      421 gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc
      481 tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga
      541 cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat
      601 ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa
      661 cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt
      721 attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat
      781 tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa
      841 acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt
      901 tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc
      961 atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt
     1021 aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc
     1081 agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc
     1141 taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc
     1201 agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc
     1261 tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg
     1321 ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa
     1381 tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat
     1441 tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc
     1501 ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt
     1561 taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc
     1621 ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc
     1681 tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat
     1741 tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt
     1801 ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta
     1861 tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc
     1921 ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa
     1981 aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa
     2041 agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag
     2101 tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat
     2161 tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg
     2221 atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg
     2281 aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga
     2341 tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa
     2401 aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt
     2461 atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta
     2521 ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat
     2581 cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc
     2641 atttatcggc tggcatatct tagcattagc aactgctggt attggttatc tatggctcag
     2701 cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa
     2761 gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa
     2821 aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt
     2881 tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc
     2941 tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa
     3001 aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt
     3061 aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa
     3121 agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac
     3181 atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact
     3241 tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac caaacaaggt
     3301 tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat
     3361 cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc
     3421 taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt
     3481 aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt
     3541 acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact
     3601 gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg
     3661 cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg
     3721 tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt
     3781 gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg
     3841 cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg
     3901 atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt
     3961 gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg
     4021 acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc
     4081 agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag
     4141 tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa
     4201 gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg
     4261 gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata
     4321 tttttcttct aattctgcct ttgtatgata attctcttca acagccctta cgatggctac
     4381 ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt
     4441 acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt
     4501 cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt
     4561 tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg
     4621 tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac
     4681 aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat
     4741 ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa
     4801 agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg
     4861 catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc
     4921 agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag
     4981 ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc
     5041 actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc
     5101 atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc
     5161 tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc
     5221 ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt
     5281 gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa
     5341 tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa
     5401 gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc
     5461 ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc
     5521 tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta
     5581 aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca
     5641 aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc
     5701 aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac
     5761 agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac
     5821 ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca
     5881 cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga
     5941 aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt
     6001 gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt
     6061 tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt
     6121 tt
//
LOCUS       DAZBWR010000136         6075 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000136 DAZBWR010000000
VERSION     DAZBWR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.141"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1102
                     /gene="yidC"
                     /locus_tag="ACHGT5_002564"
     CDS             152..1102
                     /gene="yidC"
                     /locus_tag="ACHGT5_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN7218506.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1281..2255)
                     /gene="lacD"
                     /locus_tag="ACHGT5_002565"
     CDS             complement(1281..2255)
                     /gene="lacD"
                     /locus_tag="ACHGT5_002565"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN7218507.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(2252..2710)
                     /locus_tag="ACHGT5_002566"
     CDS             complement(2252..2710)
                     /locus_tag="ACHGT5_002566"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN7218508.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(2707..4170)
                     /locus_tag="ACHGT5_002567"
     CDS             complement(2707..4170)
                     /locus_tag="ACHGT5_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN7218509.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(4152..5102)
                     /gene="pfkB"
                     /locus_tag="ACHGT5_002568"
     CDS             complement(4152..5102)
                     /gene="pfkB"
                     /locus_tag="ACHGT5_002568"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN7218510.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            5245..5994
                     /locus_tag="ACHGT5_002569"
     CDS             5245..5994
                     /locus_tag="ACHGT5_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN7218511.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         1842 a         1316 c         1045 g         1872 t
ORIGIN      
        1 atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa gaacgaatat
       61 ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta tagttaagtt
      121 tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact ggttattggg
      181 atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg attcaaacgg
      241 aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg gccaagcgat
      301 tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg caatgactgt
      361 gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa caatgatcca
      421 atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacagcaaa agctaaaagc
      481 tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag tatatcgcga
      541 aaatggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc aaatgccgat
      601 cttctctgcc ttgtatttta cggcacgcta cacagaaggt atccgggaat catcttttta
      661 cggcatcgat ctaggctcac caagtatggt gcttgtggcc atcgcagggg tcgcttatct
      721 gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga cgatgcgtac
      781 gatgctgatc gtctctcctg ctatgatcgt cttcatgtca atcagtgcgc cagccggtgt
      841 tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca tcacaaacgt
      901 tatgatgaaa ccacgtttga aagcacaagt ggcagaagag ttgaaacaaa acccgccaaa
      961 acaagtcgtc actccaagaa aagacgtgac agaatcagca aagccagcta acaacaaaca
     1021 agcaacaaaa caaatcaaac aaccaaaaaa cagtacaaac aacaaaggcc gcaacgcagg
     1081 aaagcagcag aagcggaagt agatagtaaa taagagtgta ataaaaattc attaacagtt
     1141 ttagcaatta aaaatatcga ccaagatttt tgaaaaagaa atctggtcga tatttttatt
     1201 taagtgaatg tttaatttga atcatcgatt gatcgctatc taattgcagc agcatctttt
     1261 tatgaagaaa caactttttc ttatctatca acgtagacag gtgttgctgt ttcttctaat
     1321 agtgtactta attctttaat attttgtttt ccttcttcta acatccaact actcgctttt
     1381 tcttcgcctt cggtaacaaa aggtgccact cccttcgccc atgttgctcg cccacataag
     1441 acaccattga atgtcgaacc tgattgtttt gcaaaacgta atgtatcttg aaatagcgta
     1501 gcattgactc ccgcacttaa aaaaataaac ggttgttcag tcactttcga ttgttcatag
     1561 aaatacgcgg atgcctctgc ttgactataa caagtctctt gcccatatcc ttccacaaat
     1621 ctcatattca ctggcacttc tactttcaat acatccactt gataacgttc ttttgaaaat
     1681 tctcgcatgg cttcaataac tttatgtggt tttacttttg cataggcaag agaagacgga
     1741 tcgccaatag ttgcatcata agaaagaatc tctaaaaaga aaggtatgcc ttctgcttcg
     1801 cattctgaac caatccgttc tataactatt tgtttttgca cattaatctc tgctggttca
     1861 tcaacgtcat agtacaataa aaatttacaa gcatccgcac cagcttcttt taaccttttc
     1921 accgaccaaa tattcaataa atcaggtaat cttcccggta ttttcgtatc atagccggtt
     1981 ttttcataag ctagcagtaa gccacattct ttctctcgaa gcgctgctgc aggcaggccg
     2041 tattctggat caagtaaaat agctgaggca tagggagtca ggtattgaga aaccatctgt
     2101 ttaaattgga tgatgtcttc tgatgtagct tctttatatt ggttgatcat tttcttcaaa
     2161 gcaccacgct ggtcgattgc taatgcgcta ataattccag cttcatccgt caatcgcttc
     2221 aaacgttcct gtttttctat actcatcttc ttcatagatc ttcacctatt tctttagtta
     2281 atattgtggc tatttctgtt tttgattttg cttttctcaa ctgttctgta acctcttttc
     2341 gcatcaatga tcgtgcaacg agagacaata atttcaaatg agtcgttcct gcttctgaat
     2401 ctggaataaa taacgcaaaa ataaaatcaa ctgactgtct atctaaactg ttccattcaa
     2461 ttcctttgtt cagtgtggcg ataatcatac taggttcgtt gattgtttga gacttcgcat
     2521 gagggatagc aaaacctgac attattcctg tagtcccttc tttttctctt tctagaagtt
     2581 tttgatatac ttctttttcg tcaagagcga tccctaaaca gaatgtttgc tttgctaaaa
     2641 acttaaatgc ttcttcttga ctatccaatg gctgttgtaa aaaaatgttc tcttcatgaa
     2701 tgattgtcat gataagtctc ctgctcttcc tttttcgata actgtacgat ttgctgcttt
     2761 attatccatc ttcttgtctt tatcagttac tcttttcttt tggattaatc ctagtatcac
     2821 tccaccaacg atcgaaccaa taagaatcgc tgtaacccat tggatttttc ccgtcactac
     2881 aggtaacact aaaaaaccac catgtggtgc tggaacttgg acgccaaaca aataagttaa
     2941 gacagcagca attgatgagc ctaacatcat cgtgggtaac actcggatcg ggtctttcgc
     3001 agcaaacgga atagctcctt ccgtaatatg agttgatcct aaaataaaat taaccaaacc
     3061 tgcattacga tcattggtat caaaatattt accaaacaat aaagtagcaa aaccagtaat
     3121 caacggtggt gcaatacaag ctgctgacac accagccata aaagtcgtgt ttccttgtgc
     3181 caaaagagct gttcctgtta catatgccgc cttattaacc ggtcctccca tatcaaacgc
     3241 acacatacag ccaacaatca cgccaagaac aagtggattc gaatgttcaa atccagctaa
     3301 aaagctcatc attccttcat ttatagcctt cattggacct gccagcaaag acatcacgat
     3361 gccaataaat gctacaccaa taactggata taagaagatt gccttcaagc catctagttg
     3421 tttaggtaaa attgtcagta gcttttgcaa aaataagata aaataacctg ctaagaaccc
     3481 agctaaaata cctcctaaaa atccagtacc gccattcatg gctacgattc caccaacaaa
     3541 tccaacgact aaccctggac gtttcgcgat cccttctgca atataagctg ctaaaactgg
     3601 aaccatcatt cccattgctg catcgccaac tgcttttacc attgccgctg tagcattgta
     3661 ttgtgcatga tctggatcaa aagaatagat tccccaaacg gcaaatgaaa tagcaatcaa
     3721 tactccgcca gctaccacga aaggcaacat atgagagaca ccgttcatta aatttttata
     3781 gatgctacga ccaaaattag caccactcat cgcttctggt tctaaagtat cagcagcttc
     3841 attatctgct aagacagtac cttttcctgc caatgcttcg tcaatcagcc gttgtgcatc
     3901 cttgattcct gtactgacag aaacatcaat gactcgtttt cccgcaaatc tttctgaatg
     3961 gacatcttta tctgcggcaa taatgacagc atctgcctgt ttgatttctt cagccgttaa
     4021 ttcattttcg atcccaattt gtccatgggt ctccactttg atcttgatgc cttttttcct
     4081 agctgcttgt tctaatgcct cttgcgccat gtaagtatgc gcaattcctg tcggacaacc
     4141 ggttgctgca attagttgat attttcccat gtttcaaacc tctcttcctt gattttgatt
     4201 tgtttactta attcctccac atccaaaaaa tcagtcagtc ctttacgaaa agcggttgag
     4261 ctgcctgctg ctatgctttt acgtaacgtt tcatcaagcg gtcgtcgatt catatatcct
     4321 gctaaaaaag cgcctaacat ggtatcacct gcacatgctg tattgacaac ttttcctttt
     4381 ggcgagtttc ctcgaaacac cctgtctttc gtaaaaagaa tcgctccttt ctcaccgagg
     4441 gataacaaaa cattttctgc acctaactct actagtcgtt gtccataaac aaagtaatct
     4501 tcatctgtaa ccatttttcg tccgaaccaa gaagaaagtt cttcctcgtt cggtttcagc
     4561 aaaaacggtc gatagggtaa acaatccatg gcttcttgat cactactatc aataatcaaa
     4621 tgaatcccct tttcctgaca aatccgactg atttctatta aaacctttgg agacagccct
     4681 cttggtaaac tcccagaaac acaaagataa tcttctgctt gtaatgctct tatttgattt
     4741 aaaaaattat ggacagctgt ctgcggtatt ttaggtcctt gattcactaa tttgtattct
     4801 tctcccgttt gattgacttg tgtaaataca ttgatacgcg tcataccagg cacttcaata
     4861 aactctgttt ggatttgttt ctgctgtaaa tagtcttcta tatactttcc tgtaaaccca
     4921 gcagaaaaac ctaatgccgt acttgggaaa tctagcattt ttaatactag tgacacattc
     4981 accccttttc cgtttgcctg aatgtcatcc tcttttgtgc ggtttaccat aaaaggatgc
     5041 atctcctctg tttcaataaa tagatcaatt gccaaattca tcgtgcatgt gtagatagac
     5101 ataaccaacg acctccttga ctctattatg aaatacgcga aagagaaaat gttttcttaa
     5161 tcgtcccaac acaaagaaaa cctttacatt tataaactcg tcgtgctatt ttttaactaa
     5221 cttttggtga aagaaggaaa gaatatggat cacaaattaa gtgaaacaga acagtacctc
     5281 tggaacttta tcgaacacca catcctagag atccctaact actccatcgt gaaactaagt
     5341 gaacaagcaa atgtctcaac agccaccatt gtgcggacaa tgaaaaagaa aggctatgaa
     5401 ggatttactt cgtttaagca ccatctaaaa gaaggagaaa acaagaacat caatttttct
     5461 ttcttagata aagtagacaa aggtatccgt cgagccattc taaaaaatga acaagaagtg
     5521 gtacgtacga tcaatatgtt ggaaatcgga aatattgagg atgcaataca aagaatcaaa
     5581 tttgctgacc gggtttttgt ctttgcacga ggattttcag aaatgattgg tcaagagatg
     5641 ctcgtaaaac ttcaattaac agggaaaaat tgtcaaatgc atactgatcc agaaatcatc
     5701 aaaagcatca gtcggaaact aactaagaaa gacagcgtta tttttgtttc tttgaacggc
     5761 gagacaaaag aactcgtcac cgccgcaaag aattgctatg atgcagagat tggttcgatt
     5821 ctagttacag ccaatcattc ctctacactg aaagactatt gcgagatcaa tctcgtaggt
     5881 ttcaaatcag aaggttctta tttccctgat tatgaagtac ggtcacgttt gcctctccag
     5941 attatagcga gaatcctgct tgacgcctat gtattgcgta tgggggagaa ttgaaaagca
     6001 gtttattatg gttggttaca gtttaaaaca tcacattttt tattctttat attgtatgga
     6061 ctcacggaaa ctttt
//
LOCUS       DAZBWR010000137         6068 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000137 DAZBWR010000000
VERSION     DAZBWR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.142"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACHGT5_002570"
     CDS             832..1566
                     /locus_tag="ACHGT5_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN7218512.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACHGT5_002571"
     CDS             1582..2751
                     /locus_tag="ACHGT5_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN7218513.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACHGT5_002572"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACHGT5_002572"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN7218514.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACHGT5_002573"
     CDS             3841..4767
                     /locus_tag="ACHGT5_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN7218515.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACHGT5_002574"
     CDS             4786..6036
                     /locus_tag="ACHGT5_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN7218516.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZBWR010000138         5911 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000138 DAZBWR010000000
VERSION     DAZBWR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.143"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(123..608)
                     /locus_tag="ACHGT5_002575"
     CDS             complement(123..608)
                     /locus_tag="ACHGT5_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218517.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(747..962)
                     /locus_tag="ACHGT5_002576"
     CDS             complement(747..962)
                     /locus_tag="ACHGT5_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN7218518.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1186..1986
                     /locus_tag="ACHGT5_002577"
     CDS             1186..1986
                     /locus_tag="ACHGT5_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN7218519.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2005..3873
                     /locus_tag="ACHGT5_002578"
     CDS             2005..3873
                     /locus_tag="ACHGT5_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN7218520.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3866..4357
                     /locus_tag="ACHGT5_002579"
     CDS             3866..4357
                     /locus_tag="ACHGT5_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN7218521.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4345..4899
                     /locus_tag="ACHGT5_002580"
     CDS             4345..4899
                     /locus_tag="ACHGT5_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN7218522.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4917..>5911
                     /locus_tag="ACHGT5_002581"
     CDS             4917..>5911
                     /locus_tag="ACHGT5_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN7218523.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2065 a          945 c         1192 g         1709 t
ORIGIN      
        1 aaatagctgt aacgaattat taggttaatg caaatgctta tttatactcc gtataacttt
       61 ctcagaaaaa atgataccaa taatgacttg ctagtactgt aaatccgtat aatatcatct
      121 aatcaaaatg gtgttccatt tgcatctgtc atcgtaatcg atccttcggg tgacactcga
      181 taaaaaccaa cgcttccaga tcctccttgg gaacggattt gtttgctgga agctgtgaac
      241 aaataatctc gatcaatttt ttgataaaag ctaataacaa tatcgtcact ttgtaattcg
      301 gaacggtggg caataagaaa tagaagtgct ttgtcagagt tttctaatgg aagagaaggt
      361 acagtgcttt cttgcatttc agatgttgaa gaaatgctaa acgactgaat acttgattgg
      421 ctatgttgcg aatctacatt accagtagaa gtattagtaa tggaagtagt agaagtactt
      481 ttagttgaac caacctcagt tgttgaaatg gctgctgaag attctttaac agaattcttt
      541 gttttatatc cacaggcaga aaaaagtatt acagttccca acaagcctgc gtaaatccct
      601 ttatgcatga tagctcctcc taaaatagat tatctttatt cagtatatca cttaaacaaa
      661 gaaaagcaga tggtttaaaa tgaaaaaacc ttcccaataa agggaaggtt catgaagata
      721 ataacttttt taataattat agctttctaa tatctttcat caacttttct agtaatcatt
      781 gggaaaaatt tcaagcagta gatcgcacaa atacctacga cgatataaat gatcttcgca
      841 aaaatagttg ttgaaccacc agcaatcata gctactaaat caagttcgaa caagcctacc
      901 aacagccagt tcaaccctcc cacaatcaat aaagttaatg caatagcatc taaagttttc
      961 attagaattc tcctttctta aatgataaaa caattatata tcaaaaagag aaacattgct
     1021 tatataacgc tcaggaattt tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt
     1081 ccattagtac acatttttta gctgaagctg taaatttcat gtcttaattt tttttagtga
     1141 gcttttatac tggatttgta agaaaaaaga aggagaggat cacatatgtc ggattggttg
     1201 gatattgcag gaaaagtagt aattgtaact ggaggttcct caggaattgg aagaagtatc
     1261 gtcgaaaatt tgttgaagca gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt
     1321 atacagcatg aaaacctact atctttaaaa gtcgatgtgt cttctaaaac ggacatagaa
     1381 gaagggatat ataaagtaat gaaacatttc aagacgatcg atggtctagt caataatgca
     1441 ggaataaata ttccttctct tctaatagat agaaatcatc caaaaagcaa gtatgaatta
     1501 tcagaacaag tttttgataa gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc
     1561 caagcagttg gaagaatctt ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct
     1621 gaatcaggat tggaaggatc agaaggacag agctgttatg ctgctacaaa agcagcgatg
     1681 aacagtttta ctcgttcttg ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt
     1741 gttgcaccag ggatactgga agaaaccggt ttgagaacac aagaatatga agaagctctt
     1801 tcttatacga gaggtataag cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg
     1861 atccctctag gaaggagtgg taagctgcaa gaagtggcgg atctagtctg ctattatctt
     1921 tcagaaagat caagttatat cactggagtc acgactaata tttcaggagg taaaacaaga
     1981 ggataaagga taggggggac gcacgtgacg ctagtaaaca gatggtacca attattgaaa
     2041 ttactagtca ctcacaagaa agtttcaatc caggaagtgc aaaaagaaat caaaaccagt
     2101 aatcaaacca taaaaaaaga tattggggag ttgaatgaag aaattgccgg tattgcaaaa
     2161 atcatccaaa aaaacaaaca ttttgaactt gaaatttatg attttgattt ttttgatgag
     2221 atcatgcaag gaaaattgaa aacagccagt gatttcaatt cggcatccaa aagaatcgct
     2281 tatttactaa ataggctgat cgaaacagag gatttcttac gtatggatga tttgtctgaa
     2341 gaagtcggtg tcagcagagg aacagtggtc aaggatctaa agacattaaa agagatgatc
     2401 aatgattttg atgtaaagat tacaggcaca ccaaataaag gactaagaat aataggagat
     2461 gagctagaat tacgactact tttattttat tttgtttctc cctacttttc agagacgaaa
     2521 acagaacagt ttctagaaga aaaacaagca atcaaacatt ttcagaagaa gacgaatgct
     2581 agtaaacaag aaatcaccct tttaacgaaa gttatcggta tttctttgaa tagaatctct
     2641 agtaactatc tgttgaagaa gcccatcaat cgttacagtg ggtgttttga atatatccaa
     2701 gaattcaatg aactgatcaa tcagttggaa gaaatctatg aactaacttt gagccaatat
     2761 gaaaaagatt ttatcagttt tcctttgaac atcatcaaca caagtataac gatgataagg
     2821 aaagctaact ctgatttgcg cttatatttc gaccccatga tgatgcgtat tcgacgatta
     2881 gatctcattg atctggatga gtccttctta tatcaagaga tgaaacagca cctctcttta
     2941 ttgataaatc gggtaatttt tcgttatcga ttgactgatt tattttatgg tgagatagaa
     3001 aaaaagtatc ctttttctta ccaaatcgct agcgaatgca tacaagcatt agaagctatc
     3061 ttgcaacata agatctctaa agtagagatc agctacctaa cgttgtattt ggagttagca
     3121 tttcgatatc aaaaagagca tttttcctct aaaaaagtag ccattgttca tacgacagga
     3181 aaaggaacag cattaatgat ccaaagacaa atcaaaagga tattggggag ggatatacaa
     3241 ataacacatg tttcagaaga acaatatgaa aaagtagatc taaacaaata tttcgctgtt
     3301 tttacaacga ttccgttaaa aaatgtaaat gaagagacac cggtgatcca tttgactaat
     3361 ctttttgatg actcttggtt aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc
     3421 gggtttcatt ctactgtttt tttctatcaa atatggaaag aaaaaaagac gtatcaagaa
     3481 tatttaaaca aattagtcgc tcctttgatc agaggctatt atgtggatga agaatttgaa
     3541 aaaaggatct tgaaaagaga atatcagcaa agcacatttg ttaacaaaga atttgctttt
     3601 cctcacacaa ccaaccagct atctcaacac attgttttat cagtagggct aataactgat
     3661 caaggtttag cagaaacagg aggaaatgga gttatttttt tatttgccat acctgaagaa
     3721 atgacggctg aagttgagaa tgaattattt gagctatacg atatcctatt tgaactcata
     3781 aaaatgggaa tagatgagtt tattgaatta gcaccgacac aagaatcatt tttaaaacta
     3841 atcaaaagta aaggagagag acttcatgaa tgatatgtat ctattcatag ttttcgcagc
     3901 ttcagcttat gtgctgcaag cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac
     3961 atatagcgaa ctgcgaagaa aaggaaaagt agcgattggt aaacgacctg ggaaaatccg
     4021 ttcaggcacg attgttatgc tagctataga cgatcatgat caagttttag aagtcagaaa
     4081 aatgcaaggg gttacatctt tggcaaaatt tcattcatta cctgtctatg cgggtgaaga
     4141 tatccattac atcgatcgtt accatccctc agttcgagcg gaagatcgtt tgacacaagc
     4201 agctttagaa aatgccagag aagtatactt acgaatagag gcaggaaatg aaaaaagtga
     4261 acaaccttca ttgcctttta ttgctatgct caataatttt cggatatgga aaagtacgat
     4321 caaagaaaaa aggagtgaca aacgatggat atcataacaa aatttgctga gggatttatg
     4381 aggctgttcc aaacaggtgc agaaacattc ataagctgga tgacaggaat cgtaccagtt
     4441 gtcttgatgc tgatggttgc aatgaatacg ctgattgctt ttttaggtga agaccgagta
     4501 acgaaagtag ccaaattatc agcgaaaaat ccgcttactc gttaccttgt gttaccgttc
     4561 ttatctgctt ttatgttggg aaatcctatg tcatttacaa tggctcgttt ccttccagaa
     4621 tactacaaac caagctatta tgctgctcaa gctcaatttt gtcatacgag caatggtgta
     4681 ttcccccata ttaatcctgg agaattgttt gtatggatgg ggattgctca agggatcgaa
     4741 accttagggc taaattcgat ggagctggct atccgatact tgcttgtggg actgctgatg
     4801 aactttattg gtggttggat aactgatttt actactggat ttgtatgcag acagcaagga
     4861 atcgttctta gcaaaaaagt agagctttca gttgattaga gaaaggagag ttttaaatgt
     4921 atggtgatat taagatcgta agaggaaacg gcgggtatgg aggtcctttg attattaggc
     4981 cgacaaaaga taaacacaag ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg
     5041 tagataaaat agaagaactg acaggtatgg agtctatcaa tgggttcaaa acatcaatac
     5101 ctgatagtga aatcgcgctt gcaatcgttg actgtggagg gactcttaga tgtggaattt
     5161 atcctaaaaa agggatacca accatcaata tcgtttccac aggaaaaagt ggcccattag
     5221 cacaatatat cactgaagat atttatgttt cagcagtagg gttagaacaa atacagccac
     5281 taatggaaac tgatgataac aaagaaacag taaatactgc atctccagat aaaaagtaca
     5341 aatatgatac ttctaaaaag ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa
     5401 aaattggaat gggcgcggga aaagtagttg ctacattcaa tcaatcagca agagaagcag
     5461 tagatacgat gatccggaca gtgatccctt ttatggcttt cgtttccctt ttgattggtg
     5521 tgatacaagg ttctgggatc ggtaatgtat ttgctaacat tatggcacct ttagcaggaa
     5581 atgcagttgg attaacaatc attggcttta tttgttcact gccattttta tctcctttgc
     5641 ttggtccagg ggcagttatt agccaagtga ttggaacatt gatcggtgta gagattggga
     5701 aaggaaatat tcctccccaa ttagcattac cggcactatt tgctatcaat actcaaaatg
     5761 catgtgattt tattccagtc ggtctaggtt tggaagaagc aaaagctgag acagtagaag
     5821 taggtgtacc atcagttctt tattcaagat ttcttaacgg catcccacga gttatcattg
     5881 cttgggtagc aagttttgga ttatatagct g
//
LOCUS       DAZBWR010000139         5808 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000139 DAZBWR010000000
VERSION     DAZBWR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.144"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHGT5_002582"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHGT5_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN7218524.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACHGT5_002583"
     CDS             complement(2240..4426)
                     /locus_tag="ACHGT5_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN7218525.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHGT5_002584"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHGT5_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN7218526.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACHGT5_002585"
     CDS             complement(4648..5088)
                     /locus_tag="ACHGT5_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN7218527.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACHGT5_002586"
     CDS             complement(5162..5701)
                     /locus_tag="ACHGT5_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN7218528.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1503 a         1305 c         1015 g         1985 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DAZBWR010000140         5512 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000140 DAZBWR010000000
VERSION     DAZBWR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.145"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..1653
                     /locus_tag="ACHGT5_002587"
     CDS             16..1653
                     /locus_tag="ACHGT5_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218529.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1798..2538)
                     /gene="truA"
                     /locus_tag="ACHGT5_002588"
     CDS             complement(1798..2538)
                     /gene="truA"
                     /locus_tag="ACHGT5_002588"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN7218530.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2729..3364)
                     /locus_tag="ACHGT5_002589"
     CDS             complement(2729..3364)
                     /locus_tag="ACHGT5_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN7218531.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3511..4731)
                     /gene="sstT"
                     /locus_tag="ACHGT5_002590"
     CDS             complement(3511..4731)
                     /gene="sstT"
                     /locus_tag="ACHGT5_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN7218532.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4950..5312
                     /locus_tag="ACHGT5_002591"
     CDS             4950..5312
                     /locus_tag="ACHGT5_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218533.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
BASE COUNT         1801 a         1058 c          817 g         1836 t
ORIGIN      
        1 aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa tagatatcat ttttatgtta
       61 tttctaagtt ttgcttacgt catccctcta ttcatgtcta tgggaatcta tcatagtgtc
      121 aatcaggata cttactttca tttatctcga atcataggac tagataatgt atggagttct
      181 ccagtaaact tcaataactt cgatcatcat ggaacaatga tgaatatttt ttatccatgg
      241 cttactcttt atccagcttt tttattttat aaaatgatgg gaaatcttgt tttaagttat
      301 aatatctact atttttttat cactttttta acaatggtcg tttcttattt ttctatgaaa
      361 caaataaaaa acaatcgtta tatttcgctg ttattttcga ttatttatac attttccgcc
      421 tatcgtgcca ttgacatatt ccatcgtgct tcattgggag aagctgtcgc tttaacattt
      481 ttgcctttga tactaatggg gtgttacgaa atttatatta gagattacca aaaatggtac
      541 tggttgtcta ttggcatgac gctagtagtg tatactcatt tactttctgt cgctatggtt
      601 tctgtattta ttggaggaac gttgtttctt tctttttatt tttgggacca aaaaatcgct
      661 agactcttaa gtcttttaaa agctaccgtc ctcacctttt ttttaagtgc gggattctta
      721 attcctttta ttcaacaatc tagagcacaa gaattgaaag tcccactggg aaaagaatta
      781 agtggtatgg ctcctagtga tatgctgaca cacattttaa ataataacta taacaactat
      841 actattggat tattcttatt tctcggattg attggtgctt ttatttttat aaaaaaatta
      901 actgctgatg atttatttat tttctttctt ggtgtatttg ttcttttttg ttcaaccaat
      961 ttatttcctt ggcaactttt taaccataca ccagttaaga gtctacaatt tgtctggcgg
     1021 ttaaatggtt tttcttcttt atttatagcc tatacgatga gtatagtgat ctattatatt
     1081 ttttctacta aaaataattc atggaaaata atggtcttta cgtttcttgc actaatattg
     1141 catctctcag gagtaagcaa ttctatccat gctaacaaag attcattaac acttatagca
     1201 cctgaagacg ctgttgctat agctgaaaat tacaatcata ctgattatgc aaacaaagag
     1261 tcaatctatc atcctgatat gattaataat gatcaatatt tactaggtaa ccaggaaata
     1321 aatccaacaa ctcattttat gagtaacaaa cttacaattg aattagataa ttcgaataat
     1381 aaaaatactg tcttgacgac acctatttat cgttataaag ggcaagtggc ttctattaat
     1441 ggcaaactag tacaaacaaa attatctaag tttggcacaa ctgaattaac tattcctcca
     1501 ggtataaata aagtcgttat tacttatcag tacacaaaac tagcaattgc ttcaagatat
     1561 ttatctatcg ttactttgat tttattttta ctttatcgat ttactttttc taaacaaaaa
     1621 caaccgagaa gagaagtaca acattctcac tagattttta tttaagaaag actttttagc
     1681 tgtacacata aacaggctaa aaagtctttt tttatatttc tttaatttgc ttcatacatt
     1741 atttaacatc gatatttata ctgtgaaaca tgcgtttcat ccattatcaa agtattttta
     1801 tcgataataa acttcatcca aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg
     1861 ccgcactttg ctttcaaata tttcatcgat tgtttctatc ggcatggcac ctgtgccgat
     1921 ttctagtaat gtccccatga taatccgcac cattttatat aagaatccgt ctccgacaaa
     1981 agtaaaatga agcatttctc cctttcgttc gatagataat tcatcaatcg tacgcactgt
     2041 ggattttttt gattttttca atgcagaaaa accaatgaaa tcatgtgtac ccactaattt
     2101 ttcgcaagct gcattcagct tgtccatatc caactcttga gggaaataaa agctgtggtt
     2161 tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt ttttgcccac
     2221 cgcattatat cttgaatgga agcgctcagg catttcttcg attcttttga tgacaatatc
     2281 ccgcggaagg taacgattca taaagtcgat catttctgcc agtccaattt ctgtgtttgt
     2341 tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat
     2401 aatttctatc tcttcatttg tcatctgatg caatactttt tcgatttttc cttgaatggt
     2461 tttatcgtca tctccaagtc tttgccatcc tgaatatctt tttccgtcat attcgattgt
     2521 caacttgatg tttctcatat tttagtggct cctctgtctt ccatttggtc ttctctctac
     2581 ctttttaaga ttgtagcaca aaatatagct cctttcttct ctaaaacaga agctatacaa
     2641 gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca gccaccggat gaagtatccg
     2701 ctgactgttc aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaaccagg
     2761 caatgggcac ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta
     2821 atggccaaga atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac
     2881 tgatcacaga cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga
     2941 tcaagaaaag aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat
     3001 tatggaccca tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg
     3061 taaagataca atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca
     3121 cacgagatgt acttacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc
     3181 ctgaaaccac agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata
     3241 cactgatcac gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag
     3301 tcaaaaaatc actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat
     3361 ccatgatacg cctccaataa caattaccta tgcttttaat tgttattata agcgacttta
     3421 tcttgattta acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga
     3481 atcattttaa aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc
     3541 tttcaatttg attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa
     3601 caacagatca cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat
     3661 aaatccaata ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc
     3721 taatggaatc aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc
     3781 agacaaaata cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac
     3841 agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc
     3901 taacgggatc gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat
     3961 gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt
     4021 caaacaaaag aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg
     4081 gtacacaact aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata
     4141 ctctgccaat ccagctattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc
     4201 tatcggagca aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc
     4261 caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca
     4321 gaataagacg ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt
     4381 cgtcaacata gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc
     4441 ttgtaaagta ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag
     4501 caatgttccc actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt
     4561 atgcttggca attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa
     4621 gcaactgaca aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc
     4681 aaacaatatt ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa
     4741 cttctctaac aaatgaccac cctttcacaa ttttaacaat cattgtatca ttttatgcaa
     4801 agaaactaaa ggtataattt tctaaaaatt ctaacaatac aaacaaccat ttcttgctaa
     4861 ttttgtactt tttctgtcta cacgttgttt tttgagatat tctaagttag gatgttatta
     4921 atgggcaact tatcaggaaa cgttttctta tgtttttgtc caattttttt gtgaggtgtt
     4981 tattcttgaa aaaaataaag aaggagagtt atggcttcct gacaatcttt gtcgcttgta
     5041 taatgatggt tttacagcgt atattgattt cagcagtcac tattcctaca tatgcaacaa
     5101 accctggtcg attctacatc tatacatttt tacaaacttc cgttgttatt ggtgcaaatt
     5161 tgatcccact ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa gttttacatt
     5221 atttaagtcg ttttgccttt atattcctaa tagccacact agtttgcaat atcttttttt
     5281 tacgtacaag ctggcagctt gaatattcgt gattattggc ttatcctaac acctatcagt
     5341 cagaattact ttacttatgc tgtttcttgt actttgcttt tttgtagtat tccttataca
     5401 gttggatttt taaataaatt atcgaaaaca acaattaaat atctactatt atttttaaca
     5461 attggattag ttggctgctc tactctattt aataaagacc cgtttgcatt ac
//
LOCUS       DAZBWR010000141         5481 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000141 DAZBWR010000000
VERSION     DAZBWR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.146"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(181..720)
                     /locus_tag="ACHGT5_002592"
     CDS             complement(181..720)
                     /locus_tag="ACHGT5_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218534.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            complement(860..1540)
                     /locus_tag="ACHGT5_002593"
                     /pseudo
     CDS             complement(860..1540)
                     /locus_tag="ACHGT5_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(1862..2083)
                     /locus_tag="ACHGT5_002594"
     CDS             complement(1862..2083)
                     /locus_tag="ACHGT5_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218535.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2181..2426)
                     /locus_tag="ACHGT5_002595"
     CDS             complement(2181..2426)
                     /locus_tag="ACHGT5_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218536.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2666..3280
                     /locus_tag="ACHGT5_002596"
                     /pseudo
     CDS             2666..3280
                     /locus_tag="ACHGT5_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3650..4651)
                     /locus_tag="ACHGT5_002597"
     CDS             complement(3650..4651)
                     /locus_tag="ACHGT5_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN7218537.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(4810..5076)
                     /locus_tag="ACHGT5_002598"
     CDS             complement(4810..5076)
                     /locus_tag="ACHGT5_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7218538.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(5066..5422)
                     /locus_tag="ACHGT5_002599"
     CDS             complement(5066..5422)
                     /locus_tag="ACHGT5_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN7218539.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1696 a         1012 c          752 g         2021 t
ORIGIN      
        1 aaacgtagaa aattacgtct acgttttcct atttagttct atattttcat taaattttat
       61 atttgctctt ttcacttttc ggaataaatt cactgtgact cttgatacct tcttttatat
      121 attgctaatg tattttctcc attgctatga aaatagaatc aacatattta taatatctat
      181 ctaatctatt ttcatttcac cgcagcatct tattaaataa cgtgtaaata agaatataaa
      241 agttattgaa ctaattaata aagatagaat catcgtagtt ggatacgtag atgaaacctc
      301 aactatagtt cttttccccg tacctttaat agacactaaa ccattgtcat tttctatagt
      361 gctctttttc ccatttactt tcacaactgt tccccaataa ttgaatacag gtaatgtgat
      421 attttgctca tcggcatctt ctactgtata gactattttg gaattatatg ctttatgaga
      481 tgcattctcc caccgatcac caacttttac ttcatgtttc tttaaatgat ctttatattt
      541 caatgcatct aatggtgcat agtcatatag gcctccagtt cttgaggatg tataacgtgt
      601 aacatctttt ttggtcataa ctaaacgacc tttatacgac tgaattttat gcgaataatt
      661 tgcttctgtt gctgcagtca cagtaaaaat aataagaact attcctacaa ggctccacat
      721 actttttttg ttgcttgttt tccactttaa aaacactatg gttaagatta atgaaccaaa
      781 aagcacttga aaacccaagg ttctcggttc tgttgcgaag tttgaaaatc aaacgcgccc
      841 tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt tcagtagaca
      901 ccgaaaagcc gaagagcgtt ccatttcttc ggttcttttt atatattcct cgaatggtct
      961 ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta tttcgtcgtt
     1021 taataggtcg atggtcttgt tctattaaat tgttaagata cttcacagtt cggtgctctg
     1081 tcttagtata taaacccaca ctctgtaact ttctaaaggc ggagccaaga gaaggtgctt
     1141 tatcggtcac aattgctttc ggctcaccaa actgtttatg gagtcgtttt aagaaagcat
     1201 aggctgcttg cgtatcccgt ttctttcgta accagatatc taaggttaag ccgtccgcat
     1261 caattgcacg ataaagataa tgccaacgtc ccttaatttt gatataggtt tcgtccattt
     1321 tccatgaata gaaggattgt ctatttttct tcttccaaag ataatagagg actttgctgt
     1381 actcttatac ccaacgataa atcgtagtat gacaaacatt tattccacga tcatataaca
     1441 attcctgaac ttcacgatag cttagattgt aacgcaggta gtaaccaaca gcgacaataa
     1501 tgacgtcttt tttgaattgt ttgcctttaa aatgattcat tactctgtcc tctctgtctt
     1561 ttttctcaat tttacactaa aatagatttt ttggaaaact ttgcaacaga accgttttta
     1621 ctatatactt cttcccagaa cttgtccgtc tccttcaaat tatctctacc tctcgagttc
     1681 attgggtggt ttgcccattt attaaacatg gaatcacctt tctcatactc atatatttat
     1741 gtcctatttc aattatattt atatatgctt aaataaattt gtatcctgaa atttattgta
     1801 atatgaagct gtattttaca tatagtaacc aaagagttaa taaaataaaa agagcgaacc
     1861 attatagatc agctcttttt tcattgctgt ttttaatctt atctagaatc acttcaaatg
     1921 tttcaaaggt atttacatct aactgacata accaaagcaa gtacttcttt tctttgtaag
     1981 tccaatcaat attacggtaa gaggacatta gattatggta ccttgctctt tgttcaactg
     2041 aagccaattc ccagcgtttt tcaaatattt tttcttcttc cattattgat ccctcgcaag
     2101 acgctacctc aaacttaaat aatgggcagc gaaagtctaa ttccttagat ctaagtatag
     2161 caaacaaata tgaaaaaaac ttagaaggta gtaaaagttc ttccgtcttt tgctttgctt
     2221 ctattcttaa tgtgtttcgt tatcggtgcc ctacaaagat tttcattaat tacacactca
     2281 tttattgttg tgtattttct gacatttctc tcttgatcaa ttattttaat tccatgggca
     2341 tcctgatagt tcataggaga ggagcggcta tcagaaatag ttgcacctat ctcttgaact
     2401 tcttttacaa cttctggaaa atccatccat cctgcttcag ctgtcaaaag taataatcta
     2461 taatatttat ctttcctttt caatctaaga aatcgtcacc ccattctttt tcttctatta
     2521 taaactattt aacttttaga attcaaaaac cgtttgtcta tttagagttt accctaaata
     2581 gacaaaaata taacattgaa aaatttagag ttctaacgta tgattagggt gtattataaa
     2641 cgttatagaa aagtagggaa taaatttgaa aattgcttat attagagttt catccatcga
     2701 tcaaaatgaa caacgtcaaa ttgaagaaat gaaaaagttt ggcgctgaaa aaatttttat
     2761 cgaaaaacaa tctggagcga cgattagtca tcggccgatt ttccaggaag ctttagattt
     2821 tgtcagagag caagatattt ttattgtaga agccattgat cgacttggac gcaattatga
     2881 cgagattatc gattcagtga attatctaaa gaagaagaat gtgcgactta ttatcaccag
     2941 tttaccgatc atggctgaag caattggaaa tcctctccta gataaattca tcaaagactt
     3001 aatcatttaa atctcagcca tgattgctga acaagaacgt actgaatcga aacgacgcca
     3061 ggcacaagga attaaaatcg ctaaagcaaa cggcgtctat aaaggtcgtc ctaagttata
     3121 cagtgcagac accaaagatc ctcaacgtcg ccttgtctat aggagtatcg ttcaggatct
     3181 tgagaatggg gttgctatct caaagattgc aacagactac aatgtgacta ggcaaacaat
     3241 ttaccgaata aaaaaagaaa tcgaccaact aattgtatga tttctttttt gaatcacgta
     3301 attattaatt agctttgtca tcaatacaga caatcatttt agattcatct agctaagttg
     3361 attacattta tgttaaaaag aatttagtta acataaatat aattgatgca taaatcgtcc
     3421 ttatttaaag gcatttaaca ttgacaactt agtataatgt atcttatgta aactaataag
     3481 atttgaattt tattttatac acactcgata atagatcaat tttatggtcc cccgcattta
     3541 cgattgcaca agagaaacat aaagtcgacc tattagcatt tcttttcaat tcaaacatta
     3601 aaaaagttta tacctctgct actaaaaaat gaaattatct atttaaaatt taaaacggta
     3661 aatctgattc atccaaattg gaaaatggat cagcctgtat tggagctttg tatttctcca
     3721 taataacaga tatctttttt tcatttttta aaaaatacct tttaaatttc ttatagtcct
     3781 ctgaaatcaa aatatagcgt gatggatact catcaaaaga agaatcgtat tcttccttta
     3841 aaattcccga tttacttaat ttttctagta catcaaatac tctatcactc aagtaactcc
     3901 ttaaattgtt atcactttcc cattgaacta cttttctata agttgaatta tcagttgaaa
     3961 agatagaatt ttcatccata aacgcatata ttagcagcaa tctttcatca ttgtcaaatt
     4021 ctattggatc aaaaatatct aagttgtcat caaaatcttt atcagttccg gtaacttttt
     4081 tgttaattac tgaaggtgtc ttgtaagaaa tagaatctaa atcaaagcgt tttgattcta
     4141 aattattttg tgctttctca attaaataat tcactttttt tgtgagaaac cttctgatat
     4201 tgtctttgct atatgttcca taaaaaccct tatcttctaa agagtccctg tacttcttaa
     4261 cttctatata ctcttcagcc aaaacttgtg gaactggttc atctaagaaa aaaaccccta
     4321 cctcgccttt gctattttta taaaagtaat caatttctgc tatagttccc gatctataat
     4381 tatcaactgg agaacccaac ttatggccaa aaatcgctaa taatatatca ctattttcta
     4441 caatttgttt attaataaca ctttgcccag agctattatt tatataagta ggaatcgcat
     4501 tcgtttccca tcgaattggc atcaaaatta cttttgtatt atctgtattt tctatattcc
     4561 atctatatat ggtttcttct acttcattcc tctttgctgc tatatcacct ggtgatgcta
     4621 taaatacttt tattagattt atggttttca tttattgtac ctttctcttt atgtctcttt
     4681 aaagtaattt ttgtataaaa tttattgact tttacactat aggacaactt ttatctattt
     4741 agtacaaaaa ggcccactaa actaattgat tagttaggag cctattacag gtatattttc
     4801 aactcgcttt taagcaaatt ctactaccag ctttttgcct aatgccctag cgatatcatc
     4861 aagtttatcg aaagatacgt tatcccctct ctcaattcga gcaatagtag attgagttgt
     4921 atgtgctttt tgtgcaagct ccgcttgagt taggcctgct tcctcccgtg catgataaag
     4981 agcaaccgca gtttcgagtt ttcttccttc acggtcatat gcttctgcaa aatctttgtc
     5041 tttttgcttt tcttcaatca attttttaat catcatttgt gaaccactcc tctctaagtt
     5101 cttttgcatg tttaagttca cttattggtg ttttctccgt ttttttagta aatccatggg
     5161 taatgacata ccttccattg accacatgga aatagatagc tctttggatg tttgatccta
     5221 ccttacttct taattcgtac aaattagtgt ctaatttttt cacccatttc aaccgttgag
     5281 ctactagcat cccattcttt tctgtttctt ctattgtacg taaaagcttc gcactatctt
     5341 tttttggtaa tgtttccaac cattctaaaa attcatcatg accattaggt cttttaaatg
     5401 tttcaaaaga tattttttcc atacccaatt atatcatata ttgattttat attatagtat
     5461 ataacataaa aattcgaatt a
//
LOCUS       DAZBWR010000142         5329 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000142 DAZBWR010000000
VERSION     DAZBWR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.147"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..2336
                     /locus_tag="ACHGT5_002600"
     CDS             9..2336
                     /locus_tag="ACHGT5_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFN7218540.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2333..3247
                     /locus_tag="ACHGT5_002601"
                     /pseudo
     CDS             2333..3247
                     /locus_tag="ACHGT5_002601"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3320..3835
                     /locus_tag="ACHGT5_002602"
     CDS             3320..3835
                     /locus_tag="ACHGT5_002602"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFN7218541.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3963..5261
                     /locus_tag="ACHGT5_002603"
     CDS             3963..5261
                     /locus_tag="ACHGT5_002603"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN7218542.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
BASE COUNT         1890 a         1027 c         1056 g         1356 t
ORIGIN      
        1 gaggagggat gaaaatgatt aagaaaactc aaaataaaat ccaacgtata tgtcaaaaac
       61 attttgtttc tagactcaaa gctgtatcta aaagaaaata cttacatccc attatgattt
      121 cgatcatctt gatttttact ttcttaggga gcttatcaag tatttatgta aacgtttata
      181 cagaaacgtc aaattttgct aaccaaacga cagaggagac aaatagtcca ccctattctg
      241 aaatacagtc catagaaagc accaatcagt ctagcaacaa cgagacaacc tcaaccacat
      301 cctcgactca actgattgat ccgacagaaa aagaaatcac agcagcaaaa gcagcagggg
      361 agaaagctta ttcagaaaca ggtagaagcc agacatttag tactgttgcg gccactgcga
      421 atttcaacgg acataccata ccagatggat atacagtgat atatgccaaa gatggagtca
      481 ttgttgcggt taacggatcg atgagtgcgg ataaaactgc gttacaaggc gtaaccaaag
      541 caatcgcttt tattgataac cgtccagata tccataatct gtttattggc tccacagtaa
      601 atggtactac tatcacattc ccaaaaagta tctatatggg ggtggctacc gctggaaata
      661 cgatcgattc agttacaccc aatgcggata aaagttattc ttttacaccc tcaacatggg
      721 gagggcttcc tgccggagga ggtatgagta ctttttcaat cggtatacag ttagcaaatg
      781 atattccatt cacggggtac tcgatcaaaa atgggcagat cgttgatggt aagggaaatg
      841 tttcaaattt tccaacctat caagcagcaa cggttcaggt caatgcgaat cggacaggaa
      901 ctttttctta tacagtcaat ggtggagcag caaaaatcgg aacgtacggg actacattat
      961 ctggaatcca tgtgggtgac aaagtggtta tcactccaac agcagtaacg ggctattctt
     1021 ttgcacaaac aaacggttct ttcacagccc tagaaagtaa cgtcgatacg gtgacctata
     1081 caggcaaaca aacaccaatc actattaagt atcaagacta ttttggacag atgattgctc
     1141 cttctaaaat aatgaatctt acttacggaa gtgctgcaca agacctgacg actaatacgc
     1201 ctactatcaa cggatatacc tttacagctg tttcagcgac agaaacaaag aaccaatcag
     1261 caatgagtgt tttagctagt ttagatacaa atggaaatgt aatcgtaaag gacgcaaatg
     1321 gaaaacagat ttctgaagtc atcctatatt ataagacaaa tgcaacagta tcaatcaatg
     1381 caaatggcag taagtactat gatggtcttt ctgttgtacc gatcgttaaa tatgccttta
     1441 atgataaaac agaatcaaca tcccttttaa acaatgatct cagtattgat caaatcacat
     1501 ggaatccaac tgatttcaaa gcgatgaatg aaaagggagt tgaagtaact gcgcctactg
     1561 aaattggaac ctatacgtct tggcaattga caaaaagcgg actagctaaa ctagctgcca
     1621 gaacaaacta tttatttact atcgtgcaga catctgatgt ctatacgatt aagcaaatca
     1681 gcggtgcggt agagctcagt ggcagtaaga catatgatgg aaaagcaggt gtcccgtcaa
     1741 tccatgtcaa gctagcagag ggtgtcacta gttcgcttac tccagtacaa gtggcactta
     1801 gcagtatgga ttacacagca gatgcacaat cagcaaaaaa tatggttaat gctggcagct
     1861 atacaattaa actaacgaat agcggaatag acaaagtcaa agcagcaaat cccaagttgt
     1921 catttactga tatagccaac acatcaggaa cttataccat ctataaagct aacgcagtga
     1981 ttactgtcga tgacgctgcc tttaattatg atgcccaaag tcactcgatt tcggttggaa
     2041 atctccacgt taccggtgtt gtcttaggcg aatcattgga ttatacccta acagttaaca
     2101 gccggacaga tattggatca caaatagtaa cgattagttt aggcgaagga agtgtaaaca
     2161 ataattatac gattatcgca acagataccg ctaagttgac gatcaatccg actctcttgc
     2221 ttccttcgac aggtgggata ggtctactgc cctttgtctt tttaggactg atctttattt
     2281 tttctggctt cttttatttt atccatcgta agaaaaaagc aggtgaacag cgatgaaaaa
     2341 aacaaaaaat gtacgtgtgg atatatttgc tgttctcttt tttatcctac tggtcttttt
     2401 tataggttct ccgactgtgt cagctagaga atcacctatt acatttactt tgcataatcg
     2461 aatgttcgaa agcgataaga tgccaagtat cagtcaaaac gacggaataa atatcccgga
     2521 taactttcaa ggagtaaaag ggttgaacgg cgttactttc gctatatatg atgtctcgga
     2581 tgaattttac aagctgcggt cagaaggaag tagtgtggaa gacgcacaaa gaaaactagc
     2641 acaaaaaagt gatagtgaaa aaatactggc agaaaatgtg acaaaaacag tagacgagga
     2701 agagggcatt gcttctttta gtgcatcgga caaagatgag caaggtcgtg acgctgttta
     2761 ccgatttgcg gaaataaaga cttcagatta agtaaaagaa aaatcagcac catttgtcgt
     2821 tgtgcttcct gtgacagatt catctaatca taaattgacg accatccact tgtatccaaa
     2881 gagcgaacaa aaaatacctg ctgcactcac attaacaaaa acagtagaga ataagcagac
     2941 agattttgca gatggggata aagttcctta tctcatcacc gtaacaattc ctgagaacat
     3001 caacgagata ggaacctata cgatcaaaga tacagctgat cctcagttat ggctagaacc
     3061 agaaacgatc gattttttga ttggtggaga caagattcat acatttcaca cacaaaaaac
     3121 aaaacatggt tttgtcttat cagattctgg aaaagatctt agttcgtttg ctgggaaaaa
     3181 attaacgatc cgttaccaaa tgacattgaa agaaaacagt gaaaaaacga catttgataa
     3241 cgatgtacgt ttgacgactg acaatcaaac agtagaaaca catcttgcca tccaaacagg
     3301 aggcaaacaa tttgtcaaag tggatcggca aacgaaacaa aaactagcag atgctcaatt
     3361 tctagtcaaa aaaaatgacc aatacttagc gcaagaaaac gggataaatc attgggtagc
     3421 caaagatgat tcaagagcaa cgatttttac ttccagtaaa gatggaacat tttctgtcca
     3481 tggattgtct tttggaagtt acgaactagt ggagattaaa ccgcctaaag gatatatatt
     3541 gagtcaatca accattctct ttgaagttga aaaaggcagt tatgatccgc ttcatcctat
     3601 accattggaa atcatcaacg aaccaaaagc aaatccgacg aataatggaa aaacgccagt
     3661 tcagatgcag acaaatggtc catcaaatga aaaaaatgga ggaagctttc caaaaacaag
     3721 cgaagagatg cttggaggtt tctcaatttt agggttgttg ctagtccttt caacagggac
     3781 agcttggttt tataaaaaac aacaaaaagg aaacaatagg agggaaataa aatgaaaaac
     3841 atcaaaacaa aaatatggac gtttttagga actgctatca tgctgcttcc tttcgttctc
     3901 ggcctaggaa ctgcagaggt ttctaggccg tatcacctac accagaaaat gttacggtta
     3961 atttgcataa attgaaattc acttcagcac cagaaaatca aataaacaac ggaacggaat
     4021 tgacatttcc gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat gacatcactg
     4081 ctacgtacta tccaagtaaa gacacagcag ttccagcaga cgctacaccc ttcgcaagtg
     4141 taacgacttc tggtgaagga ttagctaacc tgactcttcc aggtaaatct gatgggaaag
     4201 atgctgtata cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg ccaaatatcg
     4261 tgttaagctt acccttttat aacccgaatt catcagaccc tgacaaacct ttagacacga
     4321 ttcacctata tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga
     4381 acttttcagg ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc
     4441 aattcgtgac cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag
     4501 cgttgacatt cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca
     4561 attatacttt agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag
     4621 aaactacttt tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg
     4681 cagatgaagc caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg
     4741 ataacacgat tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag
     4801 atgtgaacac gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc
     4861 caactgttaa attaatgaaa aaaatagcaa atcttgcctc aggagaatac ccatatgctt
     4921 ggtcagatga agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag
     4981 ggaaattttc aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac
     5041 cagaaggcta cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg
     5101 atccaacaac ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa
     5161 ctggtggtat ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag
     5221 tgatctactt caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc
     5281 atacgataaa taaacgatag tgattcaaat ggggagaaag gttgtgaca
//
LOCUS       DAZBWR010000143         5313 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000143 DAZBWR010000000
VERSION     DAZBWR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.148"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHGT5_002604"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHGT5_002604"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN7218543.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHGT5_002605"
     CDS             complement(1297..2133)
                     /locus_tag="ACHGT5_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218544.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHGT5_002606"
     CDS             complement(2317..3534)
                     /locus_tag="ACHGT5_002606"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN7218545.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHGT5_002607"
     CDS             complement(3683..3844)
                     /locus_tag="ACHGT5_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218546.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHGT5_002608"
     tRNA            3917..4000
                     /locus_tag="ACHGT5_002608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5313)
                     /locus_tag="ACHGT5_002609"
                     /pseudo
     CDS             complement(4240..5313)
                     /locus_tag="ACHGT5_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1083 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca tac
//
LOCUS       DAZBWR010000144         5207 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000144 DAZBWR010000000
VERSION     DAZBWR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.149"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACHGT5_002610"
     CDS             <1..1368
                     /locus_tag="ACHGT5_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218547.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..2989
                     /locus_tag="ACHGT5_002611"
     CDS             1355..2989
                     /locus_tag="ACHGT5_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN7218548.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="ACHGT5_002612"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="ACHGT5_002612"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN7218549.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="ACHGT5_002613"
     CDS             4077..5066
                     /locus_tag="ACHGT5_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN7218550.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DAZBWR010000145         5201 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000145 DAZBWR010000000
VERSION     DAZBWR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.150"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACHGT5_002614"
     CDS             25..696
                     /locus_tag="ACHGT5_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN7218551.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACHGT5_002615"
     CDS             755..1195
                     /locus_tag="ACHGT5_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218552.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACHGT5_002616"
     CDS             1207..1875
                     /locus_tag="ACHGT5_002616"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN7218553.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACHGT5_002617"
     CDS             1939..2562
                     /locus_tag="ACHGT5_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN7218554.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACHGT5_002618"
     CDS             2618..3004
                     /locus_tag="ACHGT5_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN7218555.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACHGT5_002619"
     CDS             3217..4608
                     /locus_tag="ACHGT5_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN7218556.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1729 a          861 c         1095 g         1516 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga t
//
LOCUS       DAZBWR010000146         5100 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000146 DAZBWR010000000
VERSION     DAZBWR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.151"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACHGT5_002620"
     CDS             <1..730
                     /locus_tag="ACHGT5_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218557.1"
                     /translation="LQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLNDLSL
                     NELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKI
                     VEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYR
                     TQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPI
                     SLKGANIHLYHIAQELHLLKKHIS"
     gene            951..2714
                     /locus_tag="ACHGT5_002621"
     CDS             951..2714
                     /locus_tag="ACHGT5_002621"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN7218558.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2798..3256
                     /locus_tag="ACHGT5_002622"
     CDS             2798..3256
                     /locus_tag="ACHGT5_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN7218559.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3365..4048
                     /locus_tag="ACHGT5_002623"
     CDS             3365..4048
                     /locus_tag="ACHGT5_002623"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN7218560.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4195..4809
                     /locus_tag="ACHGT5_002624"
     CDS             4195..4809
                     /locus_tag="ACHGT5_002624"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN7218561.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4825..5031
                     /locus_tag="ACHGT5_002625"
     CDS             4825..5031
                     /locus_tag="ACHGT5_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218562.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1820 a          810 c         1035 g         1435 t
ORIGIN      
        1 cttacaagaa gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa
       61 tgagaacatg gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa
      121 tgacctatca ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc
      181 agaaaatttt attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg
      241 acaagtattt ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg
      301 aaaaattgta gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa
      361 tgaaaatttc attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg
      421 gagacaggaa atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg
      481 aacccaattt catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg
      541 ttcattagaa gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga
      601 tgctgctagc tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt
      661 aaaaggtgca aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca
      721 tatttcttga tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga
      781 aggtttttat ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga
      841 aattttttta aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt
      901 aacaaatcta ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag
      961 tattacatgt attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg
     1021 aaatgaaaca gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag
     1081 gtgggcacgt tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat
     1141 ttgattcacc aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc
     1201 ttttaactgg tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt
     1261 ccaaccaaca tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca
     1321 gcttagcaac aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag
     1381 catttatcgg tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg
     1441 gtgttttaca atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga
     1501 atcatggcag tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca
     1561 ataacctatt taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg
     1621 aaacggtgat ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg
     1681 tccatacgga aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt
     1741 ggcatgtacc atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat
     1801 ttaatatgat catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg
     1861 ccgcagtcaa tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc
     1921 cagatcatta ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt
     1981 tggcttctca gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat
     2041 atgatcaatt atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg
     2101 gcggaacgat ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat
     2161 caagtatccc gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc
     2221 tagattgggc gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg
     2281 tagaacatgg tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata
     2341 atggaggaaa agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag
     2401 taaaagcttt tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag
     2461 aaaatgacgt agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag
     2521 aagatggtag tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt
     2581 caatgaacgt attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc
     2641 agctttatga acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct
     2701 tgaatattca ataattcatt atgctcacca tccagataat tgtttatact agtatctgga
     2761 tggttttttc attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata
     2821 tgtcaaaaag ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc
     2881 atttggggat acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa
     2941 gactggtaca tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt
     3001 tggtcaatta gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat
     3061 aaatgtgacc atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga
     3121 aggggatcgg acattagctt attggcggaa agtccatgct gagtttttta aacatgctta
     3181 taaagaagcg ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga
     3241 agtaattttc ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga
     3301 atagaaaagg gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga
     3361 tattgtggat acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga
     3421 aaacgagccg ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg
     3481 cggagcagtt ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt
     3541 tgatttaccg gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac
     3601 tgcgacagaa aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga
     3661 tgcaacttct cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca
     3721 cgaacacggt cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca
     3781 agaagatggt tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa
     3841 gacgcagaca cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt
     3901 aattatggaa ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc
     3961 tttttcagtc gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt
     4021 cgaagccgca agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa
     4081 agaaaaattg atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc
     4141 attttgtctt caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt
     4201 gaacggagtc cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc
     4261 atccctagac cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca
     4321 cccttcagtt acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag
     4381 agaaaacaag atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt
     4441 atccatatcc ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct
     4501 aatgaggaaa gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca
     4561 gtaccagcat tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag
     4621 atacggaata aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat
     4681 ttaaatgaaa cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct
     4741 gtaagccgtt tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt
     4801 cccgaataaa aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca
     4861 aaagaaatga aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa
     4921 gttccgccat tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata
     4981 gaaatgccag atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt
     5041 ccgaaaagtg aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct
//
LOCUS       DAZBWR010000147         5096 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000147 DAZBWR010000000
VERSION     DAZBWR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.152"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1385
                     /locus_tag="ACHGT5_002626"
     CDS             165..1385
                     /locus_tag="ACHGT5_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN7218563.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1382..4102
                     /locus_tag="ACHGT5_002627"
     CDS             1382..4102
                     /locus_tag="ACHGT5_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7218564.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4086..5030
                     /locus_tag="ACHGT5_002628"
     CDS             4086..5030
                     /locus_tag="ACHGT5_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN7218565.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1889 a          834 c         1017 g         1356 t
ORIGIN      
        1 gattgtgaca aaaatcatgt cacaatcttt ttttcgaata aacttatttt ttgaagtcgg
       61 ccatttatcg taatttcttt gcataatacg aacaaatttt cggttattga tgaaaaaaaa
      121 cattgaaaat ggtatgataa aagaaaaaaa ggaggaaatt tttaatggtg aaatttatcc
      181 atgcggcaga tttacatatg gatcggtcat ttgaaggact gacaactctt gataaaactg
      241 tacaggaaaa actattgaag acgaatctga ccgttttatc aaatatcatt gaacaggcaa
      301 taataaataa cgttgatttt gttttacttg caggagataa ttttcatcaa aatcgtcctt
      361 cattaaaaat acagaaacat ttttcagaac agatgaaacg gttagaaaag aaccatatac
      421 ctgtatttat aatattcgga aatcatgatt tctatcaaaa agagcggtat tggttctcct
      481 ttcctaaaaa tgtccattta tttacatccg aaacggtaga gacaaaaaaa ataacgataa
      541 aaagtggaga aactgtctct ctcagtggat ttagttatcg tcaaccgtgg attcaaaaag
      601 acaaagtgat ggaatttcct tctagagaac tcacagatta ccatattggg ttgtaccacg
      661 gggaacctgg cgtctctcaa aaaggaaact atgctccttt ccaaccctca aagatgcaag
      721 aaaagggata cgattattgg gctttagggc atatccatgt gccaacggtc ttaaatgaaa
      781 agcaaacaat tgtttatcca ggtgcgcctc aaggtcatac taaaaaggaa caagcttcta
      841 catctattct actagttgaa ttaagtaaag gaagttgcca aatacatccg attaaagtag
      901 cagaggtcta ttggaagaca aaggaaatat cattgaggaa agcaagaacg acaaaagagg
      961 tcattgttca cattcgtcag cagctttcaa aagtagagga tggatttagt ttaattgaga
     1021 taaaattaaa agattatgac catctcaata cagatgtttt ggaacggata caatcaggag
     1081 agctattaga ctatttgtta gaggaattct cagataggat aaatgatttc tttatttggc
     1141 gtatttctct catagagaat actttttata cgaagacccc acttgctgct tctgctaaat
     1201 tgatggaaca gttatttcaa tactatcaga cacctcaaga tttccaacaa attttaaatg
     1261 aagtatatag tcatcaagaa gcggctagaa tattgagcga actgccggaa tatcaagaag
     1321 aaacacttga aaaagccaag cagctgctta atcaagattt tttatttgag gaggatcagg
     1381 aatgaagatt ttaaggatcg agattgctgc ttttggaaaa tggcgtcaaa aaagttttga
     1441 cttttattca ggcaatcagc tgatctatgg gggaaacgaa gcaggaaaat caacgattta
     1501 tcaatttatt caagcaatat tatttggttt cccttcaaaa ggccgtaaaa aaaaggatta
     1561 tacaccaaaa gacggctcag cctatggcgg caaaatatgg cttaaacatc ctgtatatgg
     1621 tgaatttgct gtcgagcgct acaagcagca aaatcgcgga aaatcgaagg tgtggctggg
     1681 ggatcaagtg ggaagtgatg agttactaga aaaactgatc gcacctttga cgaaagagtt
     1741 attccagcag gtatttactt tccagcaaga gcagttgatg gaactagatc atttgagaga
     1801 aaaagagcta catgatgcgc tgatttcttt aggcatcacg ggcagttctc aagtattcca
     1861 aaagcggcaa gaatattatc agaaagcaca acaattattc aagaaaaaag gccaaaaatt
     1921 actaatcaat caaaaattaa atgaatggaa agagctgcaa gaaaaaattc atcaaaaaca
     1981 agaacaggaa gcttattttg gcaagttgat ccaacaagaa caggaatacg agtcgcaaca
     2041 gcagcagtta ttggcacaat taaaagaggc gaacaaacaa ttttcattag ccagacagca
     2101 agaacttaat ttttctttat atgaagaatg gcaatctctc aaaaaaaaca gcactacagg
     2161 tcttcctgat gagcaacttc ttttagatct ggagtctttc tccaaacggt atcagcaaac
     2221 aaatgagcgt ttggaagaat tagaagcaac actggaaaag catagcggga tggatcagca
     2281 gtcagccaga tattattttt atttagagca ggagcaggaa ctaaaagatc tccagcaact
     2341 tcaatttaca gcggaacaat taaacgaaga atggcaaaga ttacaaattg agactagaga
     2401 aacaaattca gaattgaaga ttttggaaca aaggtggcat tggcagaaaa atccgttacc
     2461 ggaagaattt ttatatgaag aagaatggaa gcatcttctg gctgaagaaa gaaggattgc
     2521 tgatctagaa agccagattt ctgtgcgtct ccaaatagct aaagaacagc aagagatgat
     2581 tgaagcagaa gtgaatgact tggaagagac ctatccagca ttattacaaa atcagtcaga
     2641 gatagcacca gtctcttcat ctaaaccgta tatgctgatg gctggcggag caataatcat
     2701 tgcaggtata tttgtaccaa tgcccttgaa agtgatcctt ttactaattg gtgcaggcat
     2761 gattggctat ttcttctaca aaaggcaacc aaaaattaaa actaaaaatg atttctctga
     2821 gataaaagga atgtggcaag aaaaactagg acaacttgat ctagccttag ctcaattagc
     2881 tgaagcagaa gaaaaacaaa agagaactgg agaagaaaaa cagattcttc agcaagaagt
     2941 atatcgtttt gcggatcatc ggcacttagg aaagatggat acgctcaaac tcatgaaaga
     3001 atatggacag gaagtcatag attatcagaa attatatggc gtatttgagc ggaaacataa
     3061 aagagaacag gaaatacaag aaaaactacg acaaatcgac caaaaatttg agtttgttaa
     3121 agaatggtta ccgatacaag ataagacaat ttcagaaaaa atggtattgc tttcccgttt
     3181 tatacaagag atggaacaga tgaaatttgc cagaagctat cagcaaaata ctttattgaa
     3241 gcaagaaatc aatcaattga aaaagaaaca aaaagtatgg atacaagaat acagcgaaca
     3301 attgactcaa gcaggcttat cttatccttc ggaagtacct gtctttttac aaaaagccaa
     3361 acaagccgaa cagcagatga aaagatacaa tgaattatcg gaaatgctta ctccactatt
     3421 tcctgaagag ctgacaaaag agaaattatc tgtaagactg cgccaactga aagaaaagca
     3481 atcccaactc cagcaagaag aaaaagaagc aagtgaaaat cggcaacgac tccaacttca
     3541 agtagaagag ttgcaaagag acggcacatt ggacgaactt tatcaacaag ctagccggca
     3601 aaaagcaaaa ttacaggaat tactggagga atggggagca cttgaaatcg ccgggacatt
     3661 cttaggtgat ctttcaacag agatgagtga aagacaatta ccccagttgt taagagcagc
     3721 aggtgattat ttaggtattt taacaaaagg aaattatcag aagatattac tgaaaaatga
     3781 tacactgact ttaacagacg gatatacaga atttcccatc tatgagttat ctacaggtac
     3841 gaaagatcag ctgattatgg cgatgagatt tgcttacctc tcattagaag ctaaccgttc
     3901 gatatgcccg atcatcattg atgatggctg gcttcattat gatcatcagc gaaagtatca
     3961 attagctgaa cttttagcta aatttggaga aaagtatcaa ataatttgtt tttcttctga
     4021 taaagaaatg gtaagctatt accatgagat gaaccaaatc gtaaaacaat tagaaggagc
     4081 aaataatgaa aaaaattcgt gatttagtaa tcgatgagca gtttgaacaa tttgtcctga
     4141 tcaaaagtgc agatgtaaga atcgctaaaa acggaaaaaa atttattgct tttactttcc
     4201 aagatacttc aggtactatc gatggtaaat actgggatgc ttcggaggag gaaatcaata
     4261 aatttacagc cggcacagtc gtttttctaa acggtaaaag agaggtttat caaggaaacc
     4321 ctcaagtaaa gattcttcat atgcgcttaa caagggtaga tgagccagga gagcctagtt
     4381 tatacatgga aagagcccct ttgaagaaag aagaaatgga agaagaaatc aatcaattgt
     4441 tgtttgagat caccaatgcg cattggaatc gtattgttcg ctatctactt aataaatatc
     4501 aaaaacaatt ttttgagttt ccggcagcaa aaagaaacca tcatgcattt gccagtggat
     4561 tagcttatca tactttgaca atgcttcact tagggaaatc aatcgccgat gaatatcctg
     4621 agctgaatcg agcattgttg tatgctggta tcattttgca tgatttagga aaggtgattg
     4681 aactttccgg tcctatgtcg acagaatata cgcttgcagg aaatttgatc ggtcatctgg
     4741 tattgattga cgaagaaata actaaagcct gtcttgcgtt gaaaatcagc gaagctgacg
     4801 aagatgttct cgtattacgt catatggttt tagcacacca tggcttatta gaatatggct
     4861 cacctgtacg tccaagaata atggaagcag aaatattaca tcaaatcgac aatatcgatg
     4921 cttcgatgca gatgatgttg acttcgattc gtcaaacaga accaggaaaa tacaccgatc
     4981 ggattttcgg tatggacaac cgaagtttct atgtaccaaa agatttatag caaaaattta
     5041 tttttttcat tcaaaacaaa aaaagcgaga cataagtctt cgcttttttt gttttc
//
LOCUS       DAZBWR010000148         5039 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000148 DAZBWR010000000
VERSION     DAZBWR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.153"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..1175)
                     /gene="xerS"
                     /locus_tag="ACHGT5_002629"
     CDS             complement(90..1175)
                     /gene="xerS"
                     /locus_tag="ACHGT5_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN7218566.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1524..1719
                     /locus_tag="ACHGT5_002630"
                     /pseudo
     CDS             1524..1719
                     /locus_tag="ACHGT5_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3251..3769)
                     /locus_tag="ACHGT5_002631"
     CDS             complement(3251..3769)
                     /locus_tag="ACHGT5_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN7218567.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4136..4384
                     /locus_tag="ACHGT5_002632"
     CDS             4136..4384
                     /locus_tag="ACHGT5_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218568.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            4887..>5039
                     /locus_tag="ACHGT5_002633"
     CDS             4887..>5039
                     /locus_tag="ACHGT5_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218569.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         1672 a          823 c          775 g         1769 t
ORIGIN      
        1 ataaaaatga taatgaagct tgcctttaaa gttgggcttt tatctaattt tagagacagg
       61 cttcattatt atactatttt ttactacatt tacaaagcgt tcaaagcatc aattgtagct
      121 gcattgtcta tatgagtata taaagttgtt gtatttgtac ttgtatgtcc caattgttgg
      181 gcagttaata ccaatgaatt tgtctgttga tagagtctag tagccaaagt atgacgtaat
      241 ttatgtggac tgacttggac tctgaatcct tttgaatatt ttgctaccat ccgttcgatt
      301 gcaataccgg atatacgctt ggcttctcct tgataaattg ctagaaataa agctttttcg
      361 ttttctgtta ctttgtatcg ttctttccga atagctagat aattagctaa atattccatt
      421 gcaaatgctg ctatatttac tgcgtctctc ttatttcctt ttctagttac taccacatta
      481 ttcttttcta aagataggtc ctctaaatta atatttacaa cctcggagag tcttaatcca
      541 gatcctaaaa tcaatgcatt gatcgccagg tctctttcta catctcgttg ataatagatc
      601 aaagctcgtt tgctcaaagt ttttttatat tcattggcaa tataatctag ataattttgt
      661 gtatcttcgt ctagaaataa ttttttctcg attgcgtttg cacgggcagc taacgtagtt
      721 ttatctgtga tagttgctac ttgatccata ggattatttt ctaagtatgg ttttcctaaa
      781 ggattattgg aacttttcga aagatattta aatagaacct ttaatgctgt gatatttcta
      841 ttgatagtag aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc
      901 tcttttttta cattttcctt tgtgcgacgt ttcaaatgag atttgtacaa gtttagatca
      961 taggcagtta actgtcttat cgaggtgatt gaagtatcgg gaacgataga ctcactcaga
     1021 agccattcat aaaagagctt ataatttttt agatattcta aagtagttcg aggtgattga
     1081 tctttatcct cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt
     1141 gtatctattt tttgatatat ttctgtattt ttcatgttat tcatcctttc gataagtagg
     1201 ggagaggagt atctctataa ttggactata ccaattatag agtgttttgt caattggtat
     1261 actctgaaat ttattaattt atttcaaaaa agcagataaa taggtataga tttaataatt
     1321 taaaataaat ggtatacatg aataggaaag gaaacagaag gaggcaatct taatttttta
     1381 aattgtctaa attatataga aagaacaatc tttctatata attagacata agagagacta
     1441 cggttgattt gatattaaca agcgtatatg aatgtatata caaataaaaa aggggaggga
     1501 gaagttaaag aaaattcaga gaagtggggc actcagtagt attaacaatt taaaaggaat
     1561 ttcttaaaga aaatgggttg catcctggag atacaatttt tttagataaa gaaaaaattc
     1621 aagcaacaat tcaaaaaaat cttaactgct gagattgatg tagcaatatc tcaatcatca
     1681 acaaagtacg atcctgcctt tcaaaagctt attgataaat gtttattcat tataaaaata
     1741 aaagaaaaat ttatgtcagc ttttttcatc aaagtggtaa tgataattcg tctattaatt
     1801 gtatttttta ggaaatatgg tataattgtt ttaacgaaaa ggtaattcat gatacaccaa
     1861 aaaccactag ctggcgggct agtggttttc ttttacgttc tattcagatt aacgggtagg
     1921 cttactgtct tttttggcaa taattttgca atcaagccat ttacagatgt aatgcgcgga
     1981 tacttctgct aagattgcaa aaaacatatc gaaaaggtaa ttcataatac aacctcctcc
     2041 ccttgccata ataggccggg gtagcagaca gcataaatat tataccataa ctgaaaattt
     2101 cattttagta catttcaata tattgatgaa ccagcacaat aaaacatgcc atgactaaca
     2161 gaaaaaaact tattaaacca gatataactt gtaaacatcc aactatatag aaaacgtaaa
     2221 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
     2281 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
     2341 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
     2401 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
     2461 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
     2521 ttatgaaatg aaagaccttt tacatcttgc agaaatcgtt actgggatta aactaagcaa
     2581 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
     2641 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
     2701 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
     2761 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
     2821 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
     2881 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
     2941 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
     3001 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
     3061 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
     3121 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
     3181 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
     3241 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
     3301 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
     3361 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
     3421 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
     3481 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
     3541 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
     3601 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
     3661 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
     3721 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
     3781 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
     3841 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
     3901 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
     3961 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
     4021 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
     4081 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
     4141 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
     4201 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
     4261 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
     4321 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
     4381 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
     4441 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
     4501 ccgcgaattt actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat
     4561 taagggatca agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta
     4621 aacaattata gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac
     4681 tttcttattc tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt
     4741 acgagtacta ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc
     4801 cccacttagc ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag
     4861 aagaaaacat accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag
     4921 aattcaagca acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc
     4981 gagaatatga actaacggct tcagcatttg acaaatgggt aaagcaatct aaaacgagt
//
LOCUS       DAZBWR010000149         5016 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000149 DAZBWR010000000
VERSION     DAZBWR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.154"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..1878
                     /locus_tag="ACHGT5_002634"
     CDS             163..1878
                     /locus_tag="ACHGT5_002634"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218570.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1881..3644
                     /locus_tag="ACHGT5_002635"
     CDS             1881..3644
                     /locus_tag="ACHGT5_002635"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218571.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3684..4220)
                     /locus_tag="ACHGT5_002636"
     CDS             complement(3684..4220)
                     /locus_tag="ACHGT5_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN7218572.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     regulatory      complement(4258..4356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4543..4965)
                     /gene="mgsA"
                     /locus_tag="ACHGT5_002637"
     CDS             complement(4543..4965)
                     /gene="mgsA"
                     /locus_tag="ACHGT5_002637"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN7218573.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1498 a         1013 c          972 g         1533 t
ORIGIN      
        1 ttttttttaa aaaaaatagt ctattcaatt tctaaagctt cttcataata aaactttcat
       61 ttatgaaaag gcttgttctc ttttcattct aaatttccta agctacaatg tggtagtact
      121 tgttttttca attgtggaca aataaataat aggaggaatc atatgattca gttattacgt
      181 tatgcaaaag actatcgtaa gcaaatcatt ttgggcccgt tcttcaaatt tcttgaagct
      241 gtcttcgaac ttgtcctgcc tttaatgatg gcttccttga tcgataatgg actaaaaatg
      301 aatgaccgag gaaaaatcat tgaaatgggc ttatggatgg tcgctatgtc tgtgattggt
      361 ctgatttgtg ctatcatttg ccaatactat gcttcagtag cttcacaagg atttggtaca
      421 gagttacgta atcagctaat caaaaaaatc aatactttct ctcacaacga gttgaatcat
      481 tttggaacag ataccttgat cacccgaatg acaaacgaca tcaatcagct tcagctagct
      541 ttagcaatgg tcattcgttt attgatcaga gcaccgtttt taagtatcgg gtcagtggtc
      601 atggcttttg tgatcgactg ggaagtaggt ctgtttttct tagcattatt gcctattttc
      661 tcaatcattt tattttttat catcaaaaaa accgttcctc tctaccaaaa agtgcaagaa
      721 aaactagatc agctcaatga acacgttagt cagaatctta gcggtgttcg tgtcatccgc
      781 gcatttgcgc agacaaaaaa agaaacagag aagttcgata aaagcacaaa tgatctagcg
      841 aacaattatt tacgtgtttc caatatctct gccctattat cacctgctac aaccttgatc
      901 atgaatgtcg ggatcatctg tttgctgaca gttggcggga tcaaagtaaa tattggtagc
      961 ttgcaacaag gacaagttct tgctttgatc aattatatga atcaaatgct tttggcactg
     1021 atcgttgttt caaacctcgt cgtgatcttc acacgtgccg aagcttcagg aaatcgggtg
     1081 aaagaggtgc tagacacaga aaattcgatt cttgaagctg aggtcagtac tactcccgat
     1141 ttttcttctg aagcaattat cgcttttgac catgttgact tccgttatac tcctgattct
     1201 ggtctttctc tccaaaatat tacatttcag ctgaagcgaa atacagtcct tggaatcatt
     1261 ggtccaaccg gaagcgggaa aacgactttg acacaattga ttccgcgttt ctatgatgtc
     1321 agttccggta cagttataat ggacggaatg gatgttcgtt cttggcagct ggatcatctc
     1381 cgtcagcaaa ttgctcttgt tccccaaact tctgtactat ttaccgggac tattcgtgaa
     1441 aaccttcaat ggggtaagaa agacgccaca gaaaaagaat gttgggaagc tctgaaaatc
     1501 gctcaggcag aagaatttgt ccgtcagctt ccaaatggtt tagatacaaa agtcatggaa
     1561 aatgggaaaa atttctctgg cggacaaaaa cagcgactga cgattgcaag agctttgatt
     1621 gccaagccag cattactgat tttagatgac tctttaagtg cgttggatta tcaaacagac
     1681 ttggatttgc gtaaggcgct acagacttat cttggcagta cagtgattat tatctcccaa
     1741 cgtgtccgct ctattcaaga agcacatcat atcttagtga tggatcaagg taagattgct
     1801 gctcagggta cccatgaaga attacttgct caatcagcag aatatcagga aatcgtcgca
     1861 tcacaggagg aagaatagtt atgaaagtaa aaaaattatc cttgcagacg ctccgccgat
     1921 tcattcctta ccttactgcc tatgcaaaag acatttggct agctgtgata ctaggaatca
     1981 tcaatgggat tgcaacagtt gtgatgacgt tacaaattgg tcaaagtatc gatcaaatga
     2041 ttggagaagg acaggtccgc ttcaatcgac tgttccattt acttattcta ttcggtggta
     2101 tcgttttaat caacgtagtc agtcaatggc tcatccaagt gttaagcaat cggttagctt
     2161 acttatccgt tgcaaaatta agaaaagatg catttgctca tctgaatcga ttgccacttc
     2221 gttattatga tcaaaactcc cacggaaata tcgtcagtcg atttacaaat gatatggaca
     2281 atatttccat tgcaatttct gcggccttca atcaattatt ttctggagtg gcaatcatca
     2341 ttctttcgtt tatttttatg atgcgtctaa gtccacttct gacattggtc gttctttgtt
     2401 ctacaccaat tattttcctt gtgagttgga tcgttgctcg tgcttcacaa acaaatttcg
     2461 atagacagca aaggattatt ggggatatct cgggctttgt gaccgaacga atcggtaacc
     2521 agaaaatcgt gaaagctttt cagcaagaag aagctagtca acagcaattt gaactgctaa
     2581 atgctgatct atatgaaaaa ggacaacggg cacaattttc ttcttccttg acaaacccgt
     2641 cttctcgttt tgtagatcac cttgcttatt tatcgattgg tttagttggc ggcctattgg
     2701 cattgaagag tggttcgtca atcactgttg gtgtgatttc cagctttacg atctactcaa
     2761 gtcagttttc taaaccattt atcgaacttt ctggaatcac gacacaactg caaaccgctt
     2821 tttctggtct agaacgagca tttgatttga tcgatcagcc agaagaaaag cctgatttgc
     2881 cagatgccta tgccttggaa cccaaaaata tcaaaggaaa agtctctttt gatcatgttt
     2941 ctttttctta tgaccccaag cagcgtttga tcgaagattt caattttact gcttatccag
     3001 gtcaaaccat tgctatagta ggaaaaacag gggccggaaa atccacactt gttaatttgc
     3061 tgatgcgttt ttatgacaca acaaaaggaa caattacagt cgatgaccat gacatccgct
     3121 ctatccaacg tgatacatta cgtaaaagtt ttggaatggt gttacaagat acttggctat
     3181 ttgacagtac attgcgagaa aatcttcgtt ttgggaatcc ggaagcaagt gacgcagaaa
     3241 tcaatgatgc cctaaaagcc tcttatatgt atgaatttgt agaacgtttg ccggaaaaat
     3301 tagatactaa gattggcggt caagggttaa agatatcaga tggacaacgc caactgttga
     3361 cgattgctcg aacgatgatc agtaatcccc ctatgttgat cttagatgaa gctaccagct
     3421 cagtcgatac attgacagag aaaaaaatcc aacaagcctt cttagcaatg atggaaggaa
     3481 aaaccagttt tgtgattgcc catcgcctgt ccacaatcaa aaatgcggac cagatattag
     3541 tgatggatca gggagctatt gtcgaaatgg gcacccatga tgagctgctt caaaaagatg
     3601 gttattaccg acggctctat caagcacaat tcacagcaca ataaaaacaa gcagtcttct
     3661 gggatgggaa gactgcttgt tttttattta gcgtggatcg ctaaacttct taaaattttt
     3721 ttgtaaggaa tactacgtcc tacgtaataa atggcaattg attgaatcgg taaccatatt
     3781 actgttttta acaggcgggg cgcccaaacg acccagctga acaatggcac atggtacatc
     3841 aacgcaagcc ataatggtgt caaaaataag ttgatgatca gcgtgacact aagcgtagcc
     3901 agaatggcat ttttccaagt gatctctttt cgatagaaaa agaatccgta gattgctcct
     3961 tcaataaatg cattcaatgt aaatccaata aagaatgcag atttcgagaa gagggccatt
     4021 cctactaaat cagctaaaac tgcgcctatc cctgaccaaa aaggaccaaa aagaatgccc
     4081 atgatcactt gtggaataaa tgtgacactg attttaataa atggggtctc gattgctacc
     4141 agtcgggaca aagtgaccat caatgcaatc aataagccca taatagcaat catacgtgcg
     4201 tctaaccggt gtttcttcat ttttagcatc tcctttaaga gctgctacat cgtatcatgc
     4261 agcgaatgcg aaaataaaac cgcgaacatt acgtccttcg tccaagaggc aacatcccat
     4321 cctcttagca cttaacgctt tattttctac tctgtattat ttttgggatc ttttcccaca
     4381 gaaactatag catattttca catttactgc aaatacgaaa aactaatatt attgtttata
     4441 ttatccaata cgttcgtatt atatcgcata ttatcagttt gtttagcttt tatagctaca
     4501 tataggcgaa cattaaacgt tatttttcca tacgcttatc catcaaaatg aaagtggatg
     4561 cttgtctatt tcatggacga ttcttcgaaa atctaggaag ccggcttcca gtcctcttag
     4621 tagcacctca gctgttccga tattcgtagc taacggaatc tcatagacat cagataaacg
     4681 gataagtgct gttacatctg gttcatgggg ctgtgcagcc agcgggtctc gaagaaaaat
     4741 caccaaatct aactgatcct ccgaaatcat ggctccaatt tgctgatccc cgcctaacgg
     4801 tccggattta aaccgatgga tcggcaagcc ggttgcttcc gtgatcctct gcccagtcgt
     4861 cccggtagca aaaagttcat gtttttccaa tattgtttta tacgctgttg ctaatttcac
     4921 catcataggt ttttttcggt catgagcgat caaagcgatt ttcataagag acactccttt
     4981 tgatattgtt aacatactta tttactccag tgtatc
//
LOCUS       DAZBWR010000150         5002 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000150 DAZBWR010000000
VERSION     DAZBWR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.155"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..844
                     /locus_tag="ACHGT5_002638"
     CDS             158..844
                     /locus_tag="ACHGT5_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN7218574.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            846..1790
                     /locus_tag="ACHGT5_002639"
     CDS             846..1790
                     /locus_tag="ACHGT5_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN7218575.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1989..2633
                     /gene="pcp"
                     /locus_tag="ACHGT5_002640"
     CDS             1989..2633
                     /gene="pcp"
                     /locus_tag="ACHGT5_002640"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN7218576.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2712..3989
                     /locus_tag="ACHGT5_002641"
     CDS             2712..3989
                     /locus_tag="ACHGT5_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218577.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4247..>5002
                     /locus_tag="ACHGT5_002642"
     CDS             4247..>5002
                     /locus_tag="ACHGT5_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218578.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1631 a          896 c         1060 g         1415 t
ORIGIN      
        1 aaaaagcttt accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata
       61 tttaaaaata gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat
      121 aattaaaaaa atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt
      181 aggcgtcttg attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat
      241 ggttgcttta gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact
      301 tttaggtaca agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc
      361 aacaggaacg cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt
      421 caagaaagta tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc
      481 ggcttttaac gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat
      541 ggcattgggg accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact
      601 aaaaggtatg gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat
      661 cggaggagca ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac
      721 attgggacag cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag
      781 tgtctacttc tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca
      841 ataagatgga cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc
      901 tttatatcgt gatgggactc gtattgattt acactggagt aagaaattta acagataaaa
      961 ccaatcctca tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag
     1021 gaggaagatg gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag
     1081 caattgctaa gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa
     1141 aaatgtcaga caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg
     1201 cgatcttgtt tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt
     1261 ttgcagccat gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag
     1321 atgatgcagc agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag
     1381 cttctctagg tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag
     1441 gtacgatcgt tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg
     1501 tgatatttac ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga
     1561 tcggtgcacc attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat
     1621 tgacttgcgg tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc
     1681 ctgtagcgat gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg
     1741 cgttatttat gctagttttc caaattgctt atatgattct atttaaataa tagaagagag
     1801 gggttgtgac agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt
     1861 tataagaata tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac
     1921 gctgttcaag gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg
     1981 aacaacagat gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc
     2041 cagcctatga agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag
     2101 tggaagtacc gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc
     2161 accaaccaga tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg
     2221 aacgtgtcgc aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat
     2281 tcgatacggc tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag
     2341 caatgacaaa aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa
     2401 catttgtctg caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg
     2461 caattcgagg tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag
     2521 tgggtacgcc ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag
     2581 cagcagtaga aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca
     2641 agaaacactg gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga
     2701 aagaaacagc cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca
     2761 gcaaaagcta tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct
     2821 atgcaaaatt aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca
     2881 gctctcgttt tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca
     2941 agatcaatcc gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta
     3001 gagaattaaa agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag
     3061 gcatcaacca ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc
     3121 agggaatgac aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata
     3181 tcgattttcc tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga
     3241 tgaaacagcc atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag
     3301 tagaaatgcc gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa
     3361 gtgaacacat caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact
     3421 ttgcagaaga attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac
     3481 gtaaagattg gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg
     3541 aaacatttat ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc
     3601 agatggaata ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt
     3661 ggaagcaata ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag
     3721 tcagaaatgg ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata
     3781 agaaagaccg tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg
     3841 gaaatgtgca agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc
     3901 agaaacaagc gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg
     3961 caagaaagta tctgaaggaa ttactataaa aaacatacta agattagagg ggaacatcat
     4021 tgacgaatga tgttctcctc tattttctat ttttagatta tagtgaagag tgaaagaaag
     4081 ttcgaacaaa gccttgagga ctacgagtcc ctatcaaaaa ccgaacttct ttactgtcat
     4141 tttagaatca ggtttacgta tgttggggcc atgagcccgt gtataggaaa ttggaatcga
     4201 aatgaagcag taaagttctt tcaaaactta aaaaggagga aaacaaatgc cttacattct
     4261 tgcattagat gtttcaatgg gacacagcta cagtgtccta tacaaagacg acacctgctt
     4321 atttgaagac gagattgaac acactcaaag aggatttcat gcattactcg aagagatcca
     4381 gcacttgcct gagcgtccat tgattgtttt tgaagcaacg gggatttatt ccagaccagt
     4441 cgaaaagttc tttcaagatc atcacttttc ttactgtttg ttaaacccgt tagaggcgaa
     4501 aaaacagatg gaagaaagca ccttacgcag ttggaaaaca gataaatcgg atgcccaccg
     4561 gctcgcacag actcatctta aaaatcaacg tcagccaaaa gaagtacaaa gcaattttta
     4621 cttagagatg cgcgatttag cccgttttta tcaagaaatt gagaagaaaa tcactcgtct
     4681 gcgcatggat ttacacaact gccttcaatt gacttttcca gagcttgaac agttcttttc
     4741 aaacaggctg accccttacg cattgacatt gatccgtctt tttccccatc cagattttgt
     4801 attggcatct actcggacaa aaataaaaaa taaactgatc aacgaaacac ggaaaaaaat
     4861 ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc cactatgcac agtgtgccta
     4921 tccagctgtt gaaaaagaca gtatccactg tcaaaaaacg atctattacg ccgaacttct
     4981 ccaagattta ctcgaacaaa aa
//
LOCUS       DAZBWR010000151         4965 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000151 DAZBWR010000000
VERSION     DAZBWR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.156"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACHGT5_002643"
     CDS             complement(<1..153)
                     /locus_tag="ACHGT5_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN7218579.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFR"
     gene            311..574
                     /locus_tag="ACHGT5_002644"
     CDS             311..574
                     /locus_tag="ACHGT5_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218580.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            598..786
                     /locus_tag="ACHGT5_002645"
     CDS             598..786
                     /locus_tag="ACHGT5_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218581.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(869..1054)
                     /locus_tag="ACHGT5_002646"
     CDS             complement(869..1054)
                     /locus_tag="ACHGT5_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218582.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1269..1640
                     /locus_tag="ACHGT5_002647"
     CDS             1269..1640
                     /locus_tag="ACHGT5_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN7218583.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1790..1862)
                     /locus_tag="ACHGT5_002648"
     tRNA            complement(1790..1862)
                     /locus_tag="ACHGT5_002648"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1827..1829),aa:Gln,seq:ctg)
     gene            complement(1908..2336)
                     /locus_tag="ACHGT5_002649"
     CDS             complement(1908..2336)
                     /locus_tag="ACHGT5_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218584.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2396..3154)
                     /locus_tag="ACHGT5_002650"
     CDS             complement(2396..3154)
                     /locus_tag="ACHGT5_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN7218585.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3449..3646
                     /locus_tag="ACHGT5_002651"
     CDS             3449..3646
                     /locus_tag="ACHGT5_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218586.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3752..4234
                     /locus_tag="ACHGT5_002652"
     CDS             3752..4234
                     /locus_tag="ACHGT5_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218587.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4292..4888)
                     /locus_tag="ACHGT5_002653"
     CDS             complement(4292..4888)
                     /locus_tag="ACHGT5_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN7218588.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1604 a          777 c          959 g         1625 t
ORIGIN      
        1 cctaaaagaa aaaatttgat aagtaaaata tacagcaggt aaagaggcca gtaaaactga
       61 aaacttcttt tttgtccatt ttatgtaata gaaaagaatg cttatcataa taaaaaaagg
      121 aatataaaaa acaaattgac cgatcgcgat catactattg cctccgataa ataaagtcca
      181 tgatacaaaa taacactttg tgcgaaatgg cgcaagcagt tttgtggatt tatccggtaa
      241 aatctgtatc gattttgaaa ttagaggaga aacttgctat aatcaaagtg aaagaaggag
      301 tgggattttc atggatcatg tagtgttaga aatcgatttg catttgatca ataatattcg
      361 ggtgatttac tatgttgtaa gtaacagtgt cgaacaacgt gtactgacga ataaaataaa
      421 tggtatttta gcaaagaaag acgttcaccg attcaataat ggtgaaggaa gttattatag
      481 tatcccagta gaaaaaatta tttatacgac agtcaaagtc agagaagatc tagataccaa
      541 aaaagcatat gaacctattt ttacaactta ttgattgaca gcagatggag gaaaaaaatg
      601 aatccatatg aacaacttat tcaagatttg atcgatggaa aaatcgaaaa gattgatgta
      661 aaaagagaag aattaatggc ttttcgagaa gcgtggctga agttggagaa tcgtaaatat
      721 gtggtgggaa aagcctcact gaacggcaat gtgacttatc attacgatcc gacacgttta
      781 ttttagtatg aagtattcag aaaagaacca aaggaggacg atgcagcagc attgtcctcc
      841 tttgatttta caaaagtgaa agttttactt atttgaatag atttttaaat ttgtttttga
      901 cactgtcgat atttacatct tttaaaagat tttgggcttt ctctttataa ggtcctaatt
      961 gttctttatg ttcttcgata aatcgttttg cgccatcgaa attgccgttc ttaatcattt
     1021 ccttgacttt ttgaaataat tcttcttgat tcattttgaa cacatccttt gctatcaaca
     1081 tgatttttta catggacaaa acgagaaaaa ctgatttatc cacacgtcaa taatatcaaa
     1141 taacgatcat ttttactaaa aaaacactcg attctcgctt ctaaagtagg agaaaaatct
     1201 ccaacttggg acggatggaa tttctttaac tgacccatat actgaatata taacaaggag
     1261 gtagaaagat gaaagtgatt aaatatttga ttggactggc ggcgattagt ggtattttgc
     1321 tatttggact gaaatattat actgaaggaa gttatggaga aatccctggt atacttgatc
     1381 agctaaatcc attagttgaa aaaggagagg tctatgtaaa gacacaaaaa ccagaggaag
     1441 taaatgaata tggtacagca aggtatgtgc agaaagctgc tgatgccaat gggaaagaac
     1501 gaacggttga atttaatggt ctttctgtgt taaaagaaaa tcattatttg aaaattagca
     1561 ataaaggagc acacatagaa acgtatgagg aagttcctag agaaaaagtt ccggagaaag
     1621 cattagcaat tattgggtaa atcagttttt gtattaagaa tacgcccgat tttattattc
     1681 ataatttaat tatgtaaact aaatacaagt agtaaaaata atacaataat ccccctgcaa
     1741 aaagattgtt cttttacagg gggttcattt tttcagcaag cacatcaaat tgggatggca
     1801 gggttcgaac ctacaattgc ttgattcaga gtcaagtgcc ttaccagttt ggctacatcc
     1861 catttattca acaaaataat catagcactt tcctaaaaaa acgcaagtta tttttcttga
     1921 tcggatagtt gttgctttac tcgtaaaagg cggcgtacta gtttcttttc taatccagca
     1981 ggaagggcaa tcagttctgc tactgacaag acggtgtcca ataactgata ttctttgaca
     2041 ggttcattca taatatgata gatgtttgct aaacgttcaa tatattcaaa gcgagtatac
     2101 tctgttcgag caattagaaa tttcaataaa tcggtggctt tctcgtactc ttgatctgcg
     2161 taataaacaa gagattgacg atacaatttg atttccagtt cattgagggt agcaggtctg
     2221 cgacgctcgt tccactcatt aatagagact ttaccttgaa catttccctt gcagtcacgg
     2281 acaaaaataa tcggaatttc atttggatcc atttttttat gtgtgaattt ttccattatc
     2341 tacgctccct tcaaaggttt ggtatcttga gtataaggga tttttgtttg aattactagc
     2401 ttttctccaa agttagcatc tgtccaccca ttgcagtgaa aatcgccgaa aaatcgttga
     2461 tcggtactgc tcgatttttt acaccatagg gatcgttgac ataaatattt tctttgtcat
     2521 atccagtgac tacacaagca tgaacaagag gagtgactct tacttctcca ttatttgtgg
     2581 tccaagtcat aaaatcagtt tcatctggga cttcaaaatt gatagtcaca gaagtccaga
     2641 caggatttcc tttagatagt gcttttttga tatcatcgag ttgagcatcg gtttttggaa
     2701 aagtgtgata atccgtttgg acgatttgct gagcaaggga ggcaactggg ggaacataga
     2761 cgaccatagc ccagtctccg ccactgatat caccaacaaa tccttcatta ggatcgccat
     2821 gagtcccatc tgcattgaaa acagggacgt aatccaattg ctcagctaac tggtttttag
     2881 ttgtttcata accataaaaa gataaaagca tagaaagcga agtgacttca cagccatttt
     2941 ctaaagaagg agaatcaaac tggctctcta atggaacatc tagtaaaact tctgctttct
     3001 cttttgtctt tttcgttttt tgtgttgaac ttttttgtgc agtgttcatt gtatgttctg
     3061 gagaatggat atgtaggtaa taacttgtac cgcttaataa aagagcagct gtgcccccta
     3121 gaaacaatag ttttttgaac gtttttttct tcatttgaaa tctccctttt cagctgttta
     3181 tctttcctat tataaatgta tttttattgg aaatggaagt attatatttt aatatgtagg
     3241 aaataattag tttttaagga atatgtttta attttttgaa aaaaattctt ctaaatacaa
     3301 cagctgtttt aattgtgaaa acgaaaacag tacgctataa tgcctgtaag gttgattttt
     3361 aactgtaaat aaaaatcaat gagttaaatc tccaaagaaa tagcagtggt aatgtggaga
     3421 aaagttccag cagtttagga ggattttgat gacggaagaa atgattaatt taggagaaca
     3481 atatgcatgc aaaccaattg gttttacaaa aacggtcatt ggtgaagttg tatcaaaaat
     3541 gacaaactgc gccgtggtca aagttgccca atgtgctgca gaggaccaag aattattaga
     3601 tgaaaaagca agcatggttg ttgcaaaata tgatacattt gaataaagaa aacaattgga
     3661 cgtgcggcgt gctgcaagtc caatttcttt gagaaggtaa atcagtattc ttttcctatt
     3721 ttctggagaa gaaaaagcga ggcgagttgt catggtaaca ttctttacgg ttctattgtt
     3781 tttattcgtt gttgtgatgt ttttatttct ttggctggtt cgaaaagaaa ttatttatcg
     3841 gactgtgcga aatcgatggg tctacttggt gatacccttc ttagtgtcac tagtcatttg
     3901 gtatacactc atcagtcatc ctacagcaga tgaattggca aaagggatct tgtcagcaat
     3961 gatttttatt agttttttgt tggacagtag aggaatcaca gaagaaggac ttgttttgaa
     4021 tagctttgac aaaaaaggtg ttcctttttc tgagatcaat aaaattgtat tatatcagcc
     4081 aaaaggttct aaaatcgtaa aaatgaattt tttccgaaac ggatggcgag gaccgatgct
     4141 gaaattttct gcatcattag aagaattagt tccatttttg tcccaacgct taaacgatga
     4201 ggcagagatt gatattatga ttgatccaga ataaaaacag aggttgcgac aagatgtttg
     4261 tcacaacctc tgttttttat ttcaaataaa attatccaaa tacgcggaag cgatcggcat
     4321 cgttcatgta ttctttttcc tcagccggtg tttctggtga accgaatact aattgcgaac
     4381 gtagtttcca gtttgaaggg atattccatt cttttgcaac tgcttcgtca ataactgggt
     4441 tgtaatgttg caagtttgcg cccaatcctt tatcaactaa tgctacccaa gtattagctg
     4501 ttgcgattcc gtttgattgt tctgaccaat ctgggaagtt gtctgcatat aattcaaatt
     4561 gttcttgtag accttttacc acgtcagtat ctttgaagaa taatactgta ccataaccat
     4621 ttttgaaacc agctaatttg ttttgtgtat ttgggaaagc ttctgctgga gtcaatgggc
     4681 gaagtgcttc ttcagtgatt tcccaaagtt tttcatgtgc atctccgaaa aggataactg
     4741 cgcgtgttga ttgagcattg aaagcagttg ggctttcttt aattgcttct ttgatcaaag
     4801 ttgttaattc ttcattagaa agtgaaacat tacgtcctag atgatagatt gaacggcggt
     4861 ttttcaaagt gtcagtaaaa gttgtcataa taaaatttct cctttgattt gcgttttgca
     4921 tttttttaat tgattacgaa atgaagtata gcaccttaca aaaag
//
LOCUS       DAZBWR010000152         4956 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000152 DAZBWR010000000
VERSION     DAZBWR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.157"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACHGT5_002654"
     CDS             complement(55..828)
                     /locus_tag="ACHGT5_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7218589.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACHGT5_002655"
     CDS             972..1322
                     /locus_tag="ACHGT5_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN7218590.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACHGT5_002656"
     CDS             1303..2019
                     /locus_tag="ACHGT5_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN7218591.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACHGT5_002657"
     CDS             2019..3467
                     /locus_tag="ACHGT5_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7218592.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACHGT5_002658"
     CDS             complement(3537..4046)
                     /locus_tag="ACHGT5_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218593.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="ACHGT5_002659"
     CDS             complement(4036..4761)
                     /locus_tag="ACHGT5_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218594.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1703 a          843 c          998 g         1412 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DAZBWR010000153         4934 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000153 DAZBWR010000000
VERSION     DAZBWR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.158"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..936)
                     /locus_tag="ACHGT5_002660"
     CDS             complement(25..936)
                     /locus_tag="ACHGT5_002660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN7218595.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1313..1951)
                     /locus_tag="ACHGT5_002661"
     CDS             complement(1313..1951)
                     /locus_tag="ACHGT5_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218596.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1955..3937)
                     /locus_tag="ACHGT5_002662"
                     /pseudo
     CDS             complement(1955..3937)
                     /locus_tag="ACHGT5_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4117..4782
                     /locus_tag="ACHGT5_002663"
     CDS             4117..4782
                     /locus_tag="ACHGT5_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218597.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT         1570 a          912 c          765 g         1687 t
ORIGIN      
        1 gttcatattt tttatttata ccgcttattt actacttgct gtactagaat ctgaagtgaa
       61 tgtactcaaa acattttcaa agtctttgtc tttgattttt acattagcgt ctttcaatac
      121 ttcgccaatt actttattct ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc
      181 tttcaattct tttttgtatt tatccatgtc gttgcctttg ttttggtttt tcaccatttt
      241 aaccacataa tattccgtag tataagttga agcattggta gaagtgatca catcagaaac
      301 ttgaccatct ttcaacttaa aggctgcttc tttgacttct gccggaaccg ttgtagtagt
      361 ggaatcaaat ttcacttttc cgccatcttc ttttgtcgta gtatcagttg acttgtcttt
      421 ggctaattta gagaaatcgt ctcccttatc agcagctttc ttcacatctt ttgcatcatc
      481 ttcacttgct acttggataa tttgagcttc cacttctgga tggaaagatt cccaagccgt
      541 ttttaaatct tcatcggtca atttgatatg tttctttaga ccttcttgaa aggctagact
      601 ttgtttgata ctgtctttga atgtttgttc tgtgtaacct gcagatttca attgtgaatc
      661 aaatgaatca cccaattgtt tctttgtttg atcatattgg gaatcgattt ctttggttga
      721 tacgtcatct ccatatttat cttcaaatac ttgataaacg atcaactgtt gcacagcgga
      781 atctgctcct tgcatttttt tcaagctatc cattacttct tgttcgctga ttgtaccgcc
      841 tttataacta acggctgttt tttcaccggc attcgtacag cctgacaata agccagtgcc
      901 tagtacgatg gcaagtgcta aaaatctttt tttcatttat caattctcct acttttcgtt
      961 ttttgcgaat tgctaaaaac aaaggtgttc aagatgaaaa tcatcaagaa cacctttact
     1021 aatctgctcg atacgtcctg aatagatata aggctacaca atccagaaag ttcgctcagc
     1081 agaataaatg tcaagtagca catcacacct taatatacta cttcttttgt taccgttttt
     1141 caagtctatc ttgcgttccg tatttaacgt aactaattct tagaacaaat gttatcgatt
     1201 ttgtttttct tcttccgctg ttgggatatt caaatcagac acttttatgt tcgattttcc
     1261 agacattttt ttgttgtttt tattagaaaa agtttgtttt ttcatttgtt ttctaaagca
     1321 tgatcttctt gtttcctatt ttggtcagat atggatcatg cgtgaccacg ataactaacg
     1381 taccttggga agcctgttgc gataagattt caacgacttg ctccttattt cgtttatcta
     1441 atgatccagt tggttcatcg gctaaaatga ttttgctatt cttcaataag actctcgcac
     1501 aggcgattct ttgcttctca ccaccagata atgtgtagat aggcgtactt agcttcttgt
     1561 ttatatttac ttttttcaat gcctcatact ttgctgttcg cttatctgaa acatttaaat
     1621 tcgaaaactt caatccaata tctaaattaa aatcgattgt ttcatattca atcaacccaa
     1681 aatcttgaaa aagaaaagag agggcatttc tttttatcaa gcgcttctct cttccagtta
     1741 aatgttttgt ttcttgatta aataaataaa attgccctga actgaattga tcattcaatc
     1801 ctaatatatt caataaagta cttttccctg ctccgctctc tccaactaca gtcacaaaat
     1861 ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga atgatttttt
     1921 tcgcaccttt tatcactatc aacggctcca attgttaacc tccattcatc agcaataact
     1981 gacttttctt ttctgtagac aaaataaaaa caactgaaag aataaaatca atgaagctaa
     2041 agaaaaaaat attaaaaaga tatttaggct ctctttctat aagaaaaagg aagattacac
     2101 ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc tttatacgtt tcaaagagcg
     2161 aataaccact tgaacgaaag atagcgattt tccgacaatt gtagacaaaa tatatgcata
     2221 acatgacata gctcaacaag aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt
     2281 aatgaaatga tctgcttaac actttgacag ctatctcttc agtctgagta tacggaaatg
     2341 ataactgcaa gttatcacgt aactgacttt cttcaatcag ctcaaataac ctttctttcg
     2401 acaaagagcc tatcttgatc tttaacggtg gatagatttc tccgctgaga atatttcttt
     2461 cccaatacgt actgtttttc aatgtcagta tttcaacaac cggataatgt tcgatccagg
     2521 ggatagaagg aataaatgta taaatgggtt gattgttttt tataaataga aatttcggtt
     2581 ctttttcact gatttcttga taatattctg ttatttgcgc caggtcattt tttaaatctg
     2641 tatgtctata tcgttcaggg atcaatataa cagggtgcgt ttctttttcg gttatttgga
     2701 tcaaccttcc agagacatca atcaaaggaa atttcttaag ataattcaga tttattttta
     2761 tccctctgcc aaactgttca ttttcttcat tcaagtaatc tgatacattg actaatatac
     2821 ttccgttttt attcaaagtt tcatataatt cctgatcggc tgctgatgca aaacgattgg
     2881 aataaataaa atccacgtgg tttttcccga tggttactgg ataaaataca tgatagtcct
     2941 caccagcgat ttcttttggt gtttcatggg aaacagctaa ctgtgaagaa gaaaaaacga
     3001 tagttgctaa ttcgacattg gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa
     3061 aaagaatgta ttggatatag ttgaacgctt ctcttaacga agcgattttt tcaactaata
     3121 aaagagaaat caaagagata ccctatattc cgctgaacaa gaaaacgtac atctggaaca
     3181 aataaagaaa ttgctcctta taatacataa aaataaaacc accaatgatt cctgcaatta
     3241 tgaatgtaat aatgattttc tttttcccca ataaacacca caatacttga tgtaatgaaa
     3301 agccattatt caacaataaa ctgatttctt tattcatcaa taagaaaaag gatgataaca
     3361 aaaggaataa aagaatcaat ccaatagtta aatacactcg ataattatat acgtctgtac
     3421 ttgaaataac cgaatacgaa tagctatttt caaagtcttc aaaaatatta gtggcataat
     3481 cagtgtgaaa tgcttgattg aatcgttcca caaatagtaa aatgaaccgt tcataggctt
     3541 ctttttctct cgtcatcaag aaaaattcac cttcaaaatc ttttattttg atcgattctt
     3601 caatggacgt tacgcgaaag gaggtattag aatagggtac cttctcgtga gaagatttga
     3661 catccccttg ataatacaat gtcatttcaa gctttggctg tatcttgaag ttccaagatg
     3721 tatctacttc attggattca tttactgttt ttttcatata agaaagctca gtttctgatg
     3781 agacattttc aaataccatg attagctgct catattcttc ttgcccaata aaatgttgtg
     3841 gcaatcgaat cggagtataa gtatctgttt gatggaaaaa ttcatttgtc cgatagagta
     3901 cctgtttatt tataataacc aaggcaaaac cactcatgat accggtcact atgattgtga
     3961 gtaaaactgt gaactttttt ttgattatca ccactctccc ttacttaaat gattctttga
     4021 taaaatcata ccgtttttca tcaaaaaaac gtatctatca aatgtatagg tcatgtagaa
     4081 taaatctata tactgtagaa tcgaaaggag aagaagatga acatcggaga aaaaatacga
     4141 acttaccgca gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat cggtgtttcc
     4201 gataaaacaa tctctagttg ggaaaattct cgtacaatgc cagaccttga aatgctgtca
     4261 cttttacatc agactttagg tctgccgatt gattttcctt ctattgaatc agctaccgaa
     4321 tcaatagaac caacattgcc ggatgaacct cttgttatca agaaatctta ttcatcaaaa
     4381 caacccttct cactcaaaaa gacactatct ttctttctgc tcgtcttttg tttggtctat
     4441 ctttttctta tcaattatca atataaaaat gaaacggttg atcgattcaa tccttttttg
     4501 aaaccacaaa tcggctatac cacactccct gtaggatacg tggaaaattc catcgaacat
     4561 tgggtatgta atgatatctt tgttcatggt acatggctgt cttttcacgc tggagaggta
     4621 gaaaaaggta agaatcatgc tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc
     4681 ctgctcactt gggaagccat tccctcagat taccaagaaa tcctcatcca agactatgac
     4741 gatgccttaa aaagaagccc tcaggagcca gataggcatt aagtaggaga tgaacgtaga
     4801 gatagaaaaa atcaatgcac atgatcttat caaaaaaaaa caggggtaca aattaattga
     4861 aaaagctttg agatgaaagt tcctaaataa aaaagttcag aatgataggt aaaaaaatga
     4921 ctaactattc tagt
//
LOCUS       DAZBWR010000154         4932 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000154 DAZBWR010000000
VERSION     DAZBWR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.159"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHGT5_002664"
     CDS             85..972
                     /locus_tag="ACHGT5_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN7218598.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHGT5_002665"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHGT5_002665"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN7218599.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHGT5_002666"
     CDS             2274..2720
                     /locus_tag="ACHGT5_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN7218600.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHGT5_002667"
     CDS             2993..4345
                     /locus_tag="ACHGT5_002667"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN7218601.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHGT5_002668"
     CDS             4516..4803
                     /locus_tag="ACHGT5_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN7218602.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1394 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tt
//
LOCUS       DAZBWR010000155         4890 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000155 DAZBWR010000000
VERSION     DAZBWR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.160"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..1726)
                     /locus_tag="ACHGT5_002669"
     CDS             complement(116..1726)
                     /locus_tag="ACHGT5_002669"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN7218603.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1948..2193)
                     /locus_tag="ACHGT5_002670"
     CDS             complement(1948..2193)
                     /locus_tag="ACHGT5_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218604.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2919..4259)
                     /gene="glnA"
                     /locus_tag="ACHGT5_002671"
     CDS             complement(2919..4259)
                     /gene="glnA"
                     /locus_tag="ACHGT5_002671"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN7218605.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4287..4679)
                     /locus_tag="ACHGT5_002672"
     CDS             complement(4287..4679)
                     /locus_tag="ACHGT5_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN7218606.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1439 a         1052 c          798 g         1601 t
ORIGIN      
        1 tttgctttct atcttttttc ctacaaataa taacaaatca gaaaaagaga aaagtgacaa
       61 gaaaattgct tatccccttc tcttgctact tttctctttc tatcaagttc aaaaattatt
      121 ttgtttcgtg atctgctaat gatgctccga caaatgcttt gattaatttt tgcgggcgat
      181 ttggacgaga aatcaattct gggtggaatt ggcatcccac aaagaatttt ttctctggca
      241 gctctacgat ttctactaaa cggttatctg gtgagacacc tgaaaagact agcccttgtt
      301 cttcaaacaa ttgacgatat ttgttattga actcatagcg gtgacggtga cgttcttgca
      361 caacatcggc tccatcatat gcttcagccg tcttcgttcc ttttttgatt ttacatgggt
      421 ataatccaag gcgtaaagtt ccacccaaat tctcgatgtt ttcctgatca gccattaaat
      481 caataatatt gtttgctgta tctggattcg tttcagctga tgacgcatct tctaatccaa
      541 cgacgttacg tgcaaattcg acacaagcca tctgcatacc aagacaaata cctaaaaacg
      601 gaacgtcatt ttcgcgagca taacgaatcg cttcgatttt cccttctact ccgcgatcac
      661 caaatccacc aggaaccaag attccatctg cgtctttcaa acgttcttct acattttctc
      721 tcgttaattc ttgtgctttg atccaatcga tctcgatgtc tgaattatag gcgaaaccag
      781 aatgtttcaa tgcttcaact actgagatat atgcatctgg taactcgaca tatttaccga
      841 caagcgcaat ccgtactttt ttcttcaagt tcaagacacg ttcttctaat tctttccatt
      901 ctgtcatgtc tgcttctggt gtatctagtt ttaaatggtc gcaaacaatt tgatccattc
      961 cttgtgcttg taaaactaat gggatggagt aaagtgtatc tacatctggc gactcgataa
     1021 ctgcttctgg ttcgacatca caaaattgtg ccaatttgtt tttcacgctt tgagaaacag
     1081 gttgctctgt acgtaccacc aaaatattcg gttggatacc taaaccacgt agctctttta
     1141 cactgtgctg cgttggttta gttttcattt ctccagcagc tttcaagtaa ggaatcaatg
     1201 ttgtatggat atacataaca ttatctgtac cgacatctgc tttcatttga cgaagtgctt
     1261 caaggaatgg caaagattct atatccccta ctgttccccc tacttctgta atgatcacat
     1321 ctgaatcagt cattttagct gctcgcatga ttttttcttt gatctcattt gtaatatgag
     1381 gaataacttg aacagttgct cccaagtatt cgcccttacg ttctttacgc aaaacttcag
     1441 aatagatttt tcctgtcgtc acgttggagt atttgttcaa attgatgtca ataaaacgtt
     1501 cgtagtggcc cagatccaaa tctgtttcag caccatcatc tgttacgaat acttctccgt
     1561 gctgatatgg gctcatcgtc cccggatcca cgttgatgta tggatcaaat ttttggatag
     1621 ttacttttaa gccgcggttt ttcaataagc gtcctaaaga agctgcaaca atcccttttc
     1681 cgattgatga aacgacaccg ccggtcacaa aaatatattt tgtcataagt tcaaaaactc
     1741 ctttttaatt tatgtgcagg aaaagtctca gacaataaac aaagctccct attcttcaat
     1801 aagaataggg agccgaaatt tctgtaaact tctgaccctc taaaagggtg cccaaggtgt
     1861 ataatacaag ctgactaagg gaaagtcaag tactcaaatg aatactatcc gcttacaatt
     1921 ttttcttttt cgatatcctt tgagaaatta tttcttaagt cctttatgag tgcaaaaaaa
     1981 atttaaccca cataataagc ttgatataaa catagctaac gttaaaccta agcaaatcag
     2041 aaaatcgtta ctgaaaaatt ttaagtgaaa taatagataa aaaaataaaa caaacattat
     2101 gatagataat atcccaccta acaaagaact actttttcct acttgcagca acagtttata
     2161 tagcttatcg tagaaacaat ctttttcttt cacccttatt tacctccttt acgtaaaaaa
     2221 cgtagtccac agttctcgga tttttggcat atcttttcta ttaaataata tcaaaaacaa
     2281 aacaattcca ctaatttttt caaagatgac ctttttttca acaaaagtgc cttgtaggga
     2341 ttcattgaag gtaacgaatc acataagtaa aaaataagga gatgcgacaa aagtcgtgcc
     2401 tccttatttc taaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
     2461 catcaaacat gagaagctgt ttttgaaatt atttcttgtg attcggagtt aaatggtagg
     2521 ctcacaacct ctggataaac ggtattcaaa agccagtttc tcggtattaa gccaaattta
     2581 tgcaaaaatg tagaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacgtct
     2641 ttttcaaaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagttcagc
     2701 caattcaaaa acaagaaaag ctgtttttgc ttggctattc ttattacttg ttgcagggca
     2761 gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca
     2821 aaaaacggca aaatatgagg aactattttt ccgttagttt ctctaatact caggtcaaaa
     2881 cgctgatttc acagcctcta attcacggta ttcctgtttt aataaagttc tagatattgt
     2941 tctctttccc actcggaaac agtttgtctg aatgccgccc attcgagacg ttttgcttca
     3001 acaaaatttg agaagatatg ttgtcctaat gcgtcaatca ttacattatc cttacgcaat
     3061 tctttgatag cattatggat agttgaaggt aagtcctgga tatgtgcagc ttctctttct
     3121 tcctcatcca ttacataaat gttacggtca acagctgctg gcggtgtgat ctgattacgg
     3181 ataccgtcta gtcctgcttg caacaatact gccattgcca agtatgggtt tgcagatgga
     3241 tcaactgacc gcaattccaa acgtgtggat aaaccgcgag attctggtac acgtactaat
     3301 ggcgaacgat tacggccact ccatgcgaca taaacaggcg cttcataacc tggaacgaga
     3361 cgtttataag aattgactgt tggattgcaa acggctgtgt aggcacgagc atgtttcagc
     3421 aaaccaccta agaaatgata agctgtttca cttagttcca attcaccgtc tttatcatag
     3481 aaaacatttc cttcatcatt gaacaaagac atattgcaat gcataccaga accattgatc
     3541 ccaaataacg gtttcggcat gaaagtcgcg tgaagtccat gcttgcgtgc aatcgttttg
     3601 acaacgagct tgaaggtttg gatattatca caagcatcta ccacatctgc atatttaaag
     3661 tcaatttcat gctgtcctgg cgctacttca tgatgtgaag cttccacttc aaagcctaga
     3721 ctttctaatt ccaacacgat gtcacggcgg caattttcac ctaaatctgt tggtgcaaaa
     3781 tcaaaataac cacctttatc attcaaggtc gttgtgatcc caccgtcttc gtctaattta
     3841 aataagaaga attctggttc aggtcctaaa ttgaaagagg taaaaccaag gtcgcgcata
     3901 tctttcaatg ctcgtttcaa attcccgcga ggatcgccgg caaatggtgt cccatccgga
     3961 ttatagatat cacaaattaa acgagcaact tttccatgag cactttccca cgggaagatc
     4021 atccaagtgg agagatctgg atacaaatac atatcgcttt cttcaattcg aacaaatcct
     4081 tcaatcgaag aaccgtcaaa catcatttta ttttctaata ctttgtcgat ctggctcact
     4141 ggaacttcta catttttgat cgtacctaag atgtctgtga acatcaatcg taaaaatcgt
     4201 acgttttctt cctcaatgat tcgtttgatt tcttcacctg ttatctgttt ctttaccatg
     4261 gtatccctct tcctttttta atttattcag tcaaagaatt gtctttgcaa tccttgcgac
     4321 tgataatgac ctgttttgct taacccacct tgcgccaaaa tctcatcacg aaaaatttgt
     4381 cgaacgtcat gatctgtcaa cggcttcttg ttttttgctt cttgtttttt cgcctgtgcc
     4441 atttcgtata cacgtttgat cccagccata ttcaatccct ctgaaagata gtctttaatc
     4501 tcaagcaaca tatcaatatc attcagcgaa tacatacgtc ggtttccttc gcttcgttcc
     4561 ggatgaacta aatcttgttc ttcataatag cgaatttgcc gagcagacaa atcagtcagc
     4621 ttcataactg taccaattgg aaatacggac atagatcggc gcaattcctt ttctcccatc
     4681 agacgtcccc tttcgtttta taagttggct aaagtgtacc aattaaaaaa aatattgtca
     4741 actgcatatg ttaggttttc taacatgaga tctctatttt ttataaaata acaaaaaaat
     4801 aagaacacaa aaaaggtatc tagttgattt tcgtaccaga taccttttat tttttattat
     4861 actgtactat cctttattag tgtttttatt
//
LOCUS       DAZBWR010000156         4751 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000156 DAZBWR010000000
VERSION     DAZBWR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.161"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..699)
                     /locus_tag="ACHGT5_002673"
     CDS             complement(<1..699)
                     /locus_tag="ACHGT5_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218607.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVC"
     gene            complement(907..1833)
                     /gene="glsA"
                     /locus_tag="ACHGT5_002674"
     CDS             complement(907..1833)
                     /gene="glsA"
                     /locus_tag="ACHGT5_002674"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN7218608.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1856..3283)
                     /locus_tag="ACHGT5_002675"
     CDS             complement(1856..3283)
                     /locus_tag="ACHGT5_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN7218609.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3314..4528)
                     /locus_tag="ACHGT5_002676"
     CDS             complement(3314..4528)
                     /locus_tag="ACHGT5_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN7218610.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1485 a         1000 c          822 g         1444 t
ORIGIN      
        1 acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc
       61 aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa aggacttttc
      121 atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca actcgttaca
      181 agcttcttct acatacggat ttttattttc atagccttcc caattgacat aacgtactaa
      241 agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat ttttcaaata
      301 atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt tgctcaaaga
      361 aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa
      421 aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc
      481 atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc
      541 tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga
      601 aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac
      661 ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt
      721 tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag
      781 aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca
      841 atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa
      901 gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat
      961 accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg
     1021 atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg
     1081 gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat
     1141 tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc
     1201 cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa
     1261 ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta
     1321 agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa
     1381 tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc
     1441 atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg
     1501 attcattgga tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc
     1561 ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa
     1621 taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc
     1681 ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac
     1741 ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt
     1801 tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc attttttatt
     1861 tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga
     1921 taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag
     1981 caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa
     2041 cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt
     2101 taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa
     2161 cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga
     2221 cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt
     2281 gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg
     2341 cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa
     2401 tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta
     2461 acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg
     2521 tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg
     2581 gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca
     2641 tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa
     2701 tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc
     2761 ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa
     2821 tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca
     2881 ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg
     2941 ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt
     3001 ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga
     3061 caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga
     3121 taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga
     3181 atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga
     3241 acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct
     3301 atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt
     3361 gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg
     3421 tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt
     3481 accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg
     3541 tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt
     3601 tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca
     3661 tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg
     3721 atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc
     3781 aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt
     3841 catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg
     3901 taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc
     3961 tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg
     4021 ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt
     4081 ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta
     4141 aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca
     4201 gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc
     4261 ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc
     4321 gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga
     4381 gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa
     4441 aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat
     4501 ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat
     4561 aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa
     4621 agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa
     4681 agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttttatt
     4741 tttaaagata t
//
LOCUS       DAZBWR010000157         4585 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000157 DAZBWR010000000
VERSION     DAZBWR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.162"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..254
                     /locus_tag="ACHGT5_002677"
     CDS             48..254
                     /locus_tag="ACHGT5_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218611.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            566..1285
                     /locus_tag="ACHGT5_002678"
     CDS             566..1285
                     /locus_tag="ACHGT5_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN7218612.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(1366..2049)
                     /locus_tag="ACHGT5_002679"
     CDS             complement(1366..2049)
                     /locus_tag="ACHGT5_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN7218613.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            2268..4454
                     /locus_tag="ACHGT5_002680"
     CDS             2268..4454
                     /locus_tag="ACHGT5_002680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN7218614.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         1717 a          752 c          900 g         1216 t
ORIGIN      
        1 tatgttaaaa aattactgac cttataaaag gtgatggagg gattcttatg gaaaatgaac
       61 aattgtttga agtcggcgaa acatatgcat ttaaatctga tttatttgaa tcaccaataa
      121 aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag
      181 tagaagacaa tgataccgct cataatctgc actggcagtt agttgtacgc aaatcaagta
      241 caatagaaga ataaaaacag ataaaatgtt gagagcataa agaggttgtg agtctcccgt
      301 ttagctctga gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg
      361 ccttgtgacc gaggtgttgg caggagaata ctgtttattt gaaaaaaggg ctgcgacaag
      421 acttttgtca cagcctttat tttcatgaat aggagttact tttggctact tttagagaag
      481 tagcttttaa ggagcagata aagtcaaggc ttttatttct ataaatgaag tagtaaaatg
      541 aagaacagat tttattacag gaaggatgcc gataattgtg atcattgttg caggaacgat
      601 cggtgcaggg aaaagcacac taactgaaat gcttgcccaa gatttagaaa caaaaccttt
      661 ttatgagaac gtggaagata atgaagtatt gccgctattt tattctaatc cagaaaagta
      721 tactttttta ttacagattt tctttttgaa caaacgcttt ttggcaatca aagatgcttt
      781 cagccatgat gacaacgtac tagaccgctc catctacgaa gatagtatgc tgtttcattt
      841 gaatgcagat ttagggcgtg tttctgaagt agaagtaaag cagtatgaag gtcttttaga
      901 aacgatgctg aaggagttag aagaaatttc gccacaaaag aaaccagatc tactggttta
      961 tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa aaaagaggcc gggaatatga
     1021 acagctagaa caagatcctg aactctattc ctactataaa gaactcaatc gacgatacga
     1081 agagtggtat gaacagtttg atatatgtcc caaaatagtg atcgatgggg acaaatatga
     1141 tttcgtagcc gatccggcat gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa
     1201 gaaaatgaca gaagaggcag atgcccagtc gatccgctac actacaaata acccgaaagc
     1261 aaagggaatc tttacaacta aataatataa aaactaagca aattgctcac tcgcagatat
     1321 ttctctgtga gcagcatctt gcttagtttt tttgattcat tgagatcaaa tagtgaaccg
     1381 attcgtatga taaaattgcc ataaatgaaa tgtctgagtg agggccatga tcaaaaataa
     1441 aatggaaacg atcaaaatcg aatgggcgcc aaacagatga tgacagaaaa caaggacaat
     1501 agctcctgcg ataaaggcaa gaacgacact tagatagtgg aaaaatcgat caaaatctgc
     1561 tttttcttta gaaatacaat aatcatataa gttcgttgct acccctttta aatttccagt
     1621 tgtaaataaa ttagtgtagg cacgcccgtt gattttatca aaagctaccc actggatcga
     1681 tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact
     1741 aacaaagaaa aaaatcactg cttcataata tagaacaaac aagcgccaga aatgtttttc
     1801 tttttttcta aaataatcaa ttagatactt tgttagaaga atccctaaga aaaagaaaag
     1861 ggtagacaat acttttttac caacacttag ccattctccg tcaaatgctt ggatgatcga
     1921 tagaatgata ttgccagttt gagcagaggc aaaagcacca tattggatat aagtataact
     1981 gtccatggct cctccgataa atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga
     2041 agtctgcatt tttttcatca ccttttatga atatttttgt cttccttatc ttagcataaa
     2101 cgattgaaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc ccattttcat
     2161 ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa ataaccaact
     2221 tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg aatgaaaaag
     2281 aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa aagcttcaac
     2341 aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat ggcgaagcac
     2401 ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc tctttcttta
     2461 aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca gctatcacat
     2521 atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa acgcaaacaa
     2581 tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa cgcatactaa
     2641 tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat aatttcagcc
     2701 agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg atatacaatg
     2761 aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac ttttcaaagg
     2821 atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat aatctttata
     2881 tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg cagaatatga
     2941 acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc attgataaga
     3001 atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc caagaacaaa
     3061 aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa agcgctttgg
     3121 agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc attgatgaat
     3181 ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag gaaaacagga
     3241 tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga ggatcagagc
     3301 cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc ttattaacta
     3361 attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc tataataaaa
     3421 atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt atggaagcct
     3481 atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt cagacattta
     3541 ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct tcacaaacgc
     3601 agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa gcagatagtc
     3661 catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa attgttcatt
     3721 ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg ctccggcagc
     3781 tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct cctaaatatc
     3841 aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa tcgaaatctg
     3901 cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa gaaatctatt
     3961 cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac cagagtgatc
     4021 aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc caagccttat
     4081 cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct tacagacaag
     4141 gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt agttaccgaa
     4201 atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg acagaaaaga
     4261 aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa gaagaacgaa
     4321 gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt gtacctcaaa
     4381 tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg agtcgtgtga
     4441 aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa gaaagaagaa
     4501 ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt tcgaagaagc
     4561 tatttctgga acacagttta ttcgg
//
LOCUS       DAZBWR010000158         4572 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000158 DAZBWR010000000
VERSION     DAZBWR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.163"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..301)
                     /locus_tag="ACHGT5_002681"
     CDS             complement(<1..301)
                     /locus_tag="ACHGT5_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN7218615.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            394..743
                     /locus_tag="ACHGT5_002682"
                     /pseudo
     CDS             394..743
                     /locus_tag="ACHGT5_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1015..1305)
                     /locus_tag="ACHGT5_002683"
     CDS             complement(1015..1305)
                     /locus_tag="ACHGT5_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218616.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1707..1943
                     /locus_tag="ACHGT5_002684"
     CDS             1707..1943
                     /locus_tag="ACHGT5_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218617.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1950..3902
                     /locus_tag="ACHGT5_002685"
     CDS             1950..3902
                     /locus_tag="ACHGT5_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN7218618.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="ACHGT5_002686"
     CDS             complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="ACHGT5_002686"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN7218619.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1562 a          804 c          755 g         1451 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagattt aagtagttca gttttggagt acaaaaagtg
     1501 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
     1561 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
     1621 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
     1681 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
     1741 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
     1801 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
     1861 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
     1921 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
     1981 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
     2041 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
     2101 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
     2161 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
     2221 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     2281 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     2341 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     2401 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     2461 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     2521 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     2581 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     2641 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     2701 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     2761 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     2821 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     2881 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     2941 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     3001 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     3061 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     3121 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     3181 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     3241 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     3301 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     3361 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     3421 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     3481 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     3541 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     3601 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     3661 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     3721 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     3781 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     3841 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     3901 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     3961 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     4021 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     4081 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     4141 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     4201 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     4261 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     4321 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     4381 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     4441 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     4501 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     4561 aagcaatttt tt
//
LOCUS       DAZBWR010000159         4486 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000159 DAZBWR010000000
VERSION     DAZBWR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.164"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHGT5_002687"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHGT5_002687"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN7218620.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACHGT5_002688"
     CDS             complement(1036..1956)
                     /locus_tag="ACHGT5_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN7218621.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACHGT5_002689"
     CDS             2069..2821
                     /locus_tag="ACHGT5_002689"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN7218622.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="ACHGT5_002690"
     CDS             3685..4218
                     /locus_tag="ACHGT5_002690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN7218623.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1490 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAZBWR010000160         4416 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000160 DAZBWR010000000
VERSION     DAZBWR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.165"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACHGT5_002691"
     CDS             59..913
                     /locus_tag="ACHGT5_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN7218624.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACHGT5_002692"
     CDS             1010..3259
                     /locus_tag="ACHGT5_002692"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN7218625.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="ACHGT5_002693"
     CDS             3262..>4416
                     /locus_tag="ACHGT5_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7218626.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAZBWR010000161         4350 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000161 DAZBWR010000000
VERSION     DAZBWR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.166"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..586
                     /locus_tag="ACHGT5_002694"
     CDS             149..586
                     /locus_tag="ACHGT5_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218627.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            681..1565
                     /locus_tag="ACHGT5_002695"
     CDS             681..1565
                     /locus_tag="ACHGT5_002695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN7218628.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1787..>4350
                     /locus_tag="ACHGT5_002696"
     CDS             1787..>4350
                     /locus_tag="ACHGT5_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN7218629.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT         1422 a          728 c          967 g         1233 t
ORIGIN      
        1 ctaaatcaac agtagtttaa ttattacttg atttagttct ttttatttta aaaatttttt
       61 ttacttagaa tccagccacc ctagatattt ttctgtataa tcttaaataa agaaaacgat
      121 attaaaaaat gcacgtagaa gtagataaat ggagtggttt tgcaaatttc ttttatttct
      181 agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag gaagtacaat
      241 ggatcatttg tatataaaat atattcatca attttccgtt ttggtattag gcacactaat
      301 tatagcgggg attgttgcat ttattattca aggtcgtgac atattaacag tacatcaaga
      361 gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata acaaaaagtg
      421 gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac ctaagatttt
      481 tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca ttgttttaat
      541 tttcctcagt ttcatagtat gggtatttta tcttgtcaaa aaataaatga atgtttcatg
      601 aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt aaagaaatca
      661 atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc agttcaggcg
      721 gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg gtgtttgatg
      781 aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg
      841 ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa
      901 tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca ttgggtacgg
      961 tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg atcaatcatt
     1021 tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac tattttatga
     1081 atgtcgtcgc aattggctct atccctgaag caatcaatga tgtagatgcg gagaagaaga
     1141 cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct tccacacaat
     1201 cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc agtatcttgc
     1261 tgataggact aactaactca gtaggcggtt ttgaaacatt acttccaaat gcaaaagtgg
     1321 atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat acgcttaaaa
     1381 cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc tatcttactt
     1441 gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat gttgatggag
     1501 atgaagggga taaattacct gtgacaatca aagtgttacc ctcacattta caggtttatt
     1561 gttaaaatgg aggttgtgac cgaagagttg tcaggaggac atcggttgtt cggaataagg
     1621 gactgtgaca agacttttgt cacagccttt ttttgtacaa aaaagaaatt ttcacctaac
     1681 agaaaaattt gccataaaaa tacatatttt aaatatgaaa gtacaattac aaataaatta
     1741 aaacgttaaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatatgt
     1801 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     1861 tgccgatgta gagaaaacaa gcgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     1921 tactttttca gaagaatcaa ttacttctga aactacagaa tcccaaagtg aggcgcctac
     1981 ttctaataca aggcaagaag aaaataagac aagtgagacc tcagaagtag ctgaacaaac
     2041 gtctgatagc agtgaagagg tacaggttag agttgtttca gcggatgggt acagcgaagc
     2101 tgccacaaca gaagaactga cgcagcttct cgcagacgaa tcagtagcta agattcgttt
     2161 aactcagcct ttgactttag acagggaatt ggagatcaag cgagatatcg ttatcgattt
     2221 tggcggactt gctcataatt tcggcacaca tcacatctat atcaatgaga cacctcagtc
     2281 gatcgagttt cagaatttta aaggaacggc agctcatcca ggtgggttga taccaaatat
     2341 cgatggaaat gccattatca ttgcttatat gtccgatttt tggggaaaca gatatcattt
     2401 tacgggagaa ataaaattta ccgggacact tgacttatat gatggttcta aattaggcct
     2461 gatttacgcc cctcgtgcaa ctgtcacatt agatggtgtt tccggtgtat tggatgtcca
     2521 accagtgaaa gaagggatga atgccgctcc gggtaaagct tatttttgta gaagctatca
     2581 gctaaatgta atcaatggta gtcagcttta tggaccatat ctaggaaaat tctatggttt
     2641 tctagattca ggtgatgaga cgggcagtgg aaaaacagca ccaggactcc atatcttgag
     2701 tggttcaaag gtctctcttg actatgatcg ggcagatggc gtcagcgatg aaggagaggc
     2761 tgtcgatacg ctacccggga atgtcacatt taaagtcagt ggctcaggaa gtgaattttc
     2821 ggtaaataca aagatcaata ttaccaacga caacaatcgc ggaattatcc aaatgagagg
     2881 ctcaggatca agagtcgctg tgtcgaatga tgggaaaatc acaatcaata cagcgactac
     2941 tggcggattt cgcctgcaag gagattctag tcaaatccat gtatcaagtg gtggacaaat
     3001 ctcggtcaaa atggctggag atggcgacca acctggaaac aatgggatgc gttttgtagg
     3061 gaaaggactt tctttgatcg tagaaggagc cggatcagtc atcgatattg ataagcaaag
     3121 tggtcgttct agcgcagttc gttttgagaa cggtacgcaa aaactaacag taactaatgg
     3181 aggctcattg aaggtaagaa atgcagggga tggcaaatct tatgtgaaca gactcaatca
     3241 aggaaaccaa gcgatccaat tttatgacgc ttctggtttt ctagaaagtc cagggtcagc
     3301 tgattttacg gttactggag aaaaatcaaa tatcgatata cgcgcagatt ccggagctac
     3361 cgtagataca actggagata ttgatttgaa ctttatggct ggcgaaaaaa cttatttagt
     3421 catggtcggg aaaactgggg aagataagac tggcatcatc agtggggatg tgttgaatgt
     3481 agacttagaa tctcctacct atttcgattt ccaaaacaaa cggatcggaa gcgagactaa
     3541 tggcataggt ggctgggtat ttcaaggaaa tggcgattct tctttgaaga tcgctcattc
     3601 agaaattgct ttatggcgaa aggcaggagt aacgttcgat aactttgaaa aagatccgga
     3661 ttatttttct gatatgacag acttatcgat caatggaacc gacttaggta attttgtatc
     3721 aagccctagt gcaggtttaa caaatgaatt tgctcagaca ggtgccggaa tgaaaaatta
     3781 taatcgaatc agtgccaata accagcttcc gatgattgaa tccttaagag taccaacgaa
     3841 tgcagataaa aaaatctatg ggcatgtcgt tgttcccgaa ggtgtcgagg caaagccaag
     3901 agatgcttgg acaggcgaag tagaagtgac tttacgtatt acttatgcag atacgacgaa
     3961 atcacctgta gaaattactg ctctcacaca aggacaaaca gatgaaaatg aagggacagg
     4021 atataatccg tggggagaag gcgaacaagg cggtttgttt gaggttcaag tacccaacga
     4081 agaaagacta acaacggggg atcaagtgca aatagttgct gctaaaaaag taagaggcgg
     4141 ccaagaagtg acagaactgg actctgccaa aacgacagtc gatgtgattc ctcctgagcc
     4201 tgctaaatta agtaccacat tgatcaatgc agcttctcgc tcaatcacag gtacaggcga
     4261 agcaggagct ttggtttcat tgaaaaaaaa tcaagctgtt ttgtccgaaa caactattga
     4321 tgatacaggt aaatttgttc tttctatccc
//
LOCUS       DAZBWR010000162         4332 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000162 DAZBWR010000000
VERSION     DAZBWR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.167"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHGT5_002697"
     CDS             127..579
                     /locus_tag="ACHGT5_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218630.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHGT5_002698"
     CDS             593..1282
                     /locus_tag="ACHGT5_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218631.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHGT5_002699"
     CDS             1407..3758
                     /locus_tag="ACHGT5_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN7218632.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHGT5_002700"
     CDS             3838..4233
                     /locus_tag="ACHGT5_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218633.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1525 a          548 c          867 g         1392 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt tt
//
LOCUS       DAZBWR010000163         4189 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000163 DAZBWR010000000
VERSION     DAZBWR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.168"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..877)
                     /locus_tag="ACHGT5_002701"
     CDS             complement(140..877)
                     /locus_tag="ACHGT5_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN7218634.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(1030..2370)
                     /locus_tag="ACHGT5_002702"
     CDS             complement(1030..2370)
                     /locus_tag="ACHGT5_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN7218635.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(2607..4130)
                     /locus_tag="ACHGT5_002703"
     CDS             complement(2607..4130)
                     /locus_tag="ACHGT5_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN7218636.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         1227 a          926 c          679 g         1357 t
ORIGIN      
        1 cactaagttc tctaaacttg aaaataagcc gggatctgtt tctcttgttc cgtgaaatcc
       61 cgacttattt tgaaagattc atttgctatc tatagttaat tgactagttt tttcacggac
      121 cctataggac ccatttatat tatttcaact ttttcagcca gttttccaat gtattgacga
      181 tcacagtttg ctggtctgcg ttagaaattt ctgcctctcc atctcctttt tgctcaccat
      241 agctgccaaa acctccatga tttccgccat tgattgatac atactctgtt ctttccggta
      301 aatactgctt agcttcttga taagcttcct gattcaatac tttatctttt gtcgccgtga
      361 tcgataaaac aggaacagct gattctgtca atgttccttt tttgtcggga taacttgcca
      421 tgaagaatac cccttttaaa tgtttgtctg aatgattctt attgacataa cgacttgcca
      481 ttacgccacc taatgaatgg ccaccgatca cataatcgga atttggaaaa tcttctttga
      541 ccttatccgc tttgtttccg cccatcactg ctagatcaag tggcattttg acaatagcga
      601 ctgaaaagcc gttttttgct aaatcttgcg cccaaatact ataactttct ggctctacga
      661 gagcaccagg ataaaaaata agtaatggag actttccatg attctttttc tctccttcaa
      721 aaaataagta atcttctttg ttttttcctt cttttaccaa cacttgcgct tctgacgatg
      781 gttcgtacgt attttgctgt atatagtaat aaccggctcc accgagtaat cctaaaatga
      841 tgactaagcc aattagtatt cgtctaaata ttttcacttt ctcccacctt ttctatctga
      901 gtgacccctt tttcttaatc aagtatgatt ataccttaaa aaaataagag agaaatgcaa
      961 actttccagc atcaaataaa aaaagacgga atatccattt gaatatccgc cctttttgta
     1021 tgtttatttt tatttagcac ataaacgata cacagcttct gcataaattt ccattgcttt
     1081 atacatacta cttaattccc atcgttcatt cgcctggtgc ataaagtcag gagtatcagg
     1141 gaacatcgca ccgaatgcca cacactggtt catcgtacga gcaaaagtag cgccgcctga
     1201 tacaaacggt tcagtcatgt ctccagtaat atcgcgatat gtccccaata gatttttcac
     1261 taactcactg tctagtggca cgtataatga atcaaggaag tcaaattctt cataagtcaa
     1321 cccatatttt tctgctgttt tagttaattt agctactaaa tcatctttct tgaatgtaac
     1381 agggattctc atatctacac cgatttttgt ttgttctggt gtgatttcca aacttgcaaa
     1441 gttcatcgtt agctcgccgg attgttcatc ttctgttttg cccacgacag cttcaccagt
     1501 tgcattttct tgaactaatt ttcctaagaa attgattggg ccaaaatcaa atacttctga
     1561 aagagcaatt gccagacgag aaatagcatt cactccttgt gctgcatctt tcgcgtggat
     1621 acttttacca agaacgacga tgccttcacc ttgttcttcg aaatcgaatt cgtgtttttt
     1681 caatgcttct tttacttcag aaagcttttc tccagagtaa ggagctgcat caggaacaac
     1741 attcaacgct cctccagctt ttacagaaaa ctcgttcgtt cctggtcctg tcaagtaagc
     1801 ttgcaataag cctttttctg cataaattaa aggaaattca gcatctggcg caaatccttg
     1861 cgtaattcct tcttcttttt cgttatattt ttctaaacaa cgccataaat tttcttcatc
     1921 tgtaccaaag ataaaacgaa tacgtgtctt gaattccacg ccagcatcaa tcaaagcttt
     1981 gacggcatac attgccgcaa tagaaggacc tttgtcatct tgggatccgc gaccaactaa
     2041 ccagccgtct tttatagttg gatcgaatgg ttttgtttcc caattatttt cgtcccctgc
     2101 tggaacaacg tccatatgac acaagatacc gaataattca tctccagagc caatctctga
     2161 ataaccgtaa tagccttctg gatctttaaa tgttctaaat cctagctttt cactgatttc
     2221 caatacttta tccaaagctt caatcacttt tttaccgaag ggatgtcctt gtcctgaatc
     2281 tgcttcatct agtacggaag gaattcggat caattcgctc aatgattcta atgcttgttc
     2341 atgatgttgt tctgtcacaa attttttcat gatattatcc tcctaatatg ttaaaaaggg
     2401 tcatgtcaaa ttcctccgac acaacctctt cttctcaaat aatgacgttt taagcctgct
     2461 ccatcagcaa gcagaatgtt cttaacaaat gatgaaagaa aaaacactca ctgaatggag
     2521 ctattgactt atccacgtgc ttatcgatag ccgtttatcg gtttcttcat tcttatgata
     2581 gaccttgtac ctgtatttgt ctaggtttag aacattgctg ctacacctag gatcacacag
     2641 atggctacaa ataatatcgc aattaattta ccggtaaatt tccaccacac tgaaacatcg
     2701 aaacgagcaa ttgctacagc acccattacg atagcagatg ttggtgtaat taagtttact
     2761 aaaccagatg cagattgata agcagtgatt actaaatcct taccgacgcc ggcaaattca
     2821 cccataggtc ccatgattcc cattgttgca gcagctaagc cggaagttga aggaatcaag
     2881 aatgacatag ggatatagaa aataaatgta ataatgatga atacacttga agataatgaa
     2941 cttaatcctt gttcgcccca atgtaagatg gtgtctgtga ttaatccatt gttcataacc
     3001 acttggatac cacgagcaac agctactacg attgctacac cgatcaaatc agaagcacca
     3061 gctaaaaatt cagagacaaa ttcagactct ttcatgccat aagtgattgc tactacgatg
     3121 gacattacta agaataacat agtaatttct gggaagtacc agtcacctaa tggaagcatg
     3181 cttttaccta gaaccgtacc tagtaccgga aggtttgtta accagtttgt caatgattca
     3241 aaaatcgtga aattcgcatt caaggttgtc caaggaatca aactgatgat catgatacca
     3301 aaagtaataa agaacaatac attaacatgt ttttgttttt tcgtgattac ttcttgacga
     3361 ccgattgctt caatatcgaa atgcttcata tcttctttac gttggttgta tactaatgat
     3421 tttgttggat ctttttcgat ctttgatgca taacgataca catatacaat agaaatagct
     3481 aacaaaataa cgaacatcaa gaatcgaaga ccaatacctt cacccatcga aatcccaaca
     3541 gcttgtgaag ctacgcctgt tgcaaaagga tttaccgttg aagctagaca gcctacttga
     3601 gaccctacta atacgattgc tacagctact actgtatcaa agcctacagc catcatgact
     3661 gggatcagta aagcatagaa tggaatcgtt tcttctgcca ttccgtaagt agagccgcct
     3721 aatgcaaata acagcatcaa tactggaata agcatttttt cttttccttt gaatttcttc
     3781 acaactgaag caatcccggc atctagtgcc cctgttttgt tgataatacc taaaaatcca
     3841 ccaacaacca aaatgaagaa agagattggg attgcagcag gtgtttcttt agttagctcc
     3901 cccgctggta cacctagcat cccattgatt ggcgccatga aaatatccca cagcccttga
     3961 ggattttgtt gtactcgttc atatgatcca gcgacgatgt tgcctgcatc gtctacacta
     4021 tagtgcccag ctggtataaa ccaagtaagt acagcgatta ctgcaataat aataaataag
     4081 accgtatacg ctgaaggcat ttgaaatttt ttcttttttg tttgttccat cttcttcttc
     4141 ctttcttttc cgttagagac aacaattttg gctctaatgt tattttatc
//
LOCUS       DAZBWR010000164         4076 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000164 DAZBWR010000000
VERSION     DAZBWR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.169"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2771)
                     /gene="brxC"
                     /locus_tag="ACHGT5_002704"
     CDS             complement(<1..2771)
                     /gene="brxC"
                     /locus_tag="ACHGT5_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFN7218637.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLR"
     gene            complement(2796..3371)
                     /locus_tag="ACHGT5_002705"
     CDS             complement(2796..3371)
                     /locus_tag="ACHGT5_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFN7218638.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3371..3970)
                     /locus_tag="ACHGT5_002706"
     CDS             complement(3371..3970)
                     /locus_tag="ACHGT5_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFN7218639.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1217 a          782 c          550 g         1527 t
ORIGIN      
        1 cgtaaattgt ctaattgacc ttttaaatat ttcttgaaat tttccattgt atgttcattg
       61 ttattttcat caacaatatt acgactttga aacagttctc taccgatttc gtcaaccaat
      121 ttttttgctt ttggcgaaat caccgcgctt ggttcaattt ttatcttttc actttctcgt
      181 cgtttttgaa taatcattac aatactattt ggatcatcca tccgtgaaat ttgtacattg
      241 ttataaagta aatcaatttt accagacatg aaaagtttag cgatgcacca ttccacatca
      301 agttctgtga atccatacgg ttgttttgtt tcaaaacgat cacgaattgt tttcatcgta
      361 atcgttaggt gatttctctt ttgatcattg ataaaattga aaatttcctg aactgcttgc
      421 tcatttgatg gatcttcaat aatttcattt ttgctttctt tcactaaata taagatatct
      481 tctttttctt taggcgcatc aatttggttt aatttatggt atacttcttc agccattttc
      541 tgaagtgctg attggaattt tgctttaaag tcggagcctg tcagttgaat ttctgcaccg
      601 ttatagaaaa attttgcaga tttcaacagt tcatttaatt gatcgttgat acttgcactt
      661 agttcctgta attcaccaga tttttccgaa cgaatcgcgt caaagttctt tactacactc
      721 gaataagtcg ttgttaaata aagattcatt ctagcataca ttgctatatc atctttaaat
      781 tctttatctg atggcaattc tacgaataat aagttacggg aactcttaag cgcaagttct
      841 gcctcttgac ctgctaaatc agagtatggt gaaataaaat taactgtcat ttcagcattt
      901 ccagcttgct tgtatggacg ttgatctaac acttgtgcat aagtaaacgt ataccggttt
      961 ttaaatttat ttccacctga catgcctaat ttttcatagg caaaactatt tcggtctaga
     1021 acgccgtcaa aaattcgttt agatagttct acgatcaaat cactatctgt aatattttga
     1081 tttttaatct cacgagtaat ttcctgttct tcatcagttt ggaaaatata actatctaag
     1141 ttcttccgca caaagttttc cttaattaaa aggtccaatg cttcttctac tcgtttttta
     1201 gtttccaggc ggtcttcgtt gatatttctt atcatcaagc tcgtaatatt ttcaacattt
     1261 ggtttgatgg cttgatcaac atatttaatc atgaataaaa ttcgtaagac atttacgtta
     1321 aagttatcta tatcagttgg tctttgaata tgagaattat ttgtagcttg tgtaatcact
     1381 cggctaattg ctgtatcaat ccaacgttct aacgagtaat aaaactgatc taaagaaacc
     1441 aaactggttg tatcattttc cttatttcgt tcagcggctt ctttaaacat tgctagtaat
     1501 gaacgttcac cagacgataa gtttgatcct tgatagctgt gattacgaat ctcggttaga
     1561 atttgcccca ataaattgaa ttgataggga ataaatggat aaacttctgc gtaattttca
     1621 gcatcttgga agagctttaa ttcaggacta tcaacaaaga gtaatttatt tttcaattct
     1681 gtttgaacag tttcaaattg taaggctaaa gaatcattgg caaactcttt tttcttcaaa
     1741 atacgttctt taattacttc atcggcattt gctgaagata aactcacagt tgtggcaaaa
     1801 cgatccttga tcttagaaaa atcattatca gaattgttat caaagtttgt tacgtgatca
     1861 atcgcttcct gacttgtcac taatacccaa gccttacctt cacaaattcg acccaaatct
     1921 tccacaacgg tttgtaactg caaagtcaac tcacgattac cgccgatata ttgtccgacc
     1981 tcatcgacta gaaaggcaat atgatggtct gcttctttgg aatcaagata gcttttaact
     2041 agatttgcaa agtcatcaat gcttattaaa taatttcctg atgctttttc taaaaaatta
     2101 tcaatagcgg cttcactcat aaaatcgatt ttaagtgccg cttctttgat atcatcttgg
     2161 atccaatcaa actcatggcg ctcttcttgc cattttgccc cattaatctc ttcaaaagca
     2221 gctttgaatg catcaaactt ttcttcttta attaaccgac gttcaaaatc tgctagatgt
     2281 ggaatcgtgc ttaatccttg catttcgtta aataccttta aaaaaacatt cacgattccg
     2341 tttttgtctt ttttaccacc tgcatcactt tttgaatcaa tattaaacag aataacatct
     2401 gtatttattt gtgagcaccg ttccatcgtt gccaaagtca gtggatcttt gattttccca
     2461 tcttctttga agtaatcaat ggctttcttg tcatttattt ctcgatttgc gagcaagtaa
     2521 gaaataattt ttaagaaatg agatttacct gaaccaaaga acccctgaat ccaaacaccc
     2581 attttatctg ttggtgtatt gatactccgt tggtaggcat caaaaattgt tttaaagtgt
     2641 ttttgtagtt ctttcgtaac cacgtattct tctaattctt gttgaacatt ttcatccttt
     2701 gcttgaccga ccttaataac tccttgaata tttcggtcaa ttggttttgc aaaaatatct
     2761 ttaattagca tttaatctcc taccttccat cccaactaaa ttctaaatgc tcgataataa
     2821 ttatcatctt tattggtatt aagaattgtc aaacttaatt catcatattc ccctggatac
     2881 atcatcacaa ctggtacgcg atcaaatacc tgatgcattt tatttaaaat agagtgcgct
     2941 ctaattaatg gatataattc gccaattcct gtcaggaaca caactgattt ttctggtgta
     3001 tgggcatcca catattcaaa aaatacattt tcttcctcag gtaaatccat tttcaataaa
     3061 cgaccaatgc tttccactaa tttttgtaaa ccacgtcgtt cttccatctt aatcgctttt
     3121 tcagtgaaac cgcgtttttc tagatactct aacataatca catagagatt ataaactttc
     3181 acttcataat tatcaccaac agatgttcca gatatatcat taatataatc tcttacaatt
     3241 aactcgtctt tcggatcgta gtcaaagata taatagccta cttcattacc taaaccacga
     3301 ttcaataaaa aatcttcttt ttgaattact tcattcaact tttccaaatg ctcttgaatc
     3361 gataatgaca ttataaaatt cctcccagtg ctagtacaat atctttacga ttcttgtcaa
     3421 ctaaccattg aatcaatcgt ttatctaata atatcggagt cattcgtcca tattcttttt
     3481 cccctctgac taatcctgtt tcaattagat tcgtttgata agcggcaata agccgtttaa
     3541 tcgtataatc tttccatttt gaaatatctt ctgaacttct ccgcttctct gtaaaaaaag
     3601 aacggtaatc aactggtgtt aattgcaaat ttcctttacg tagattttca gcatatacct
     3661 cagtcataaa ttcattaaaa agatcatcaa tcataaggat ggaaatcaaa ttcactaact
     3721 tttgattatc tatatttaac tgattgaaaa gttgcaatcc agcttcatcc agttgactaa
     3781 ctctcctttt cacgttctga tagatttctt tttgacggtt actgctcact gcaaaaaaaa
     3841 tattttcatg ttcatttttc tctttaattt ctatatctgt atatccttct agtcgtaatt
     3901 gaatatataa cttgaactct gaaaaccaaa agcgtttaga aactagacca gcagaatatt
     3961 ttttattcaa tagattgcct cctaatcatc tataattcaa taagtaaaca agtgtttttt
     4021 gctcatcgtt agcattcatt ttttagtatc tactatacaa tacatattta ttatag
//
LOCUS       DAZBWR010000165         4058 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000165 DAZBWR010000000
VERSION     DAZBWR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.170"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(168..1673)
                     /locus_tag="ACHGT5_002707"
     CDS             complement(168..1673)
                     /locus_tag="ACHGT5_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7218640.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1670..2356)
                     /locus_tag="ACHGT5_002708"
     CDS             complement(1670..2356)
                     /locus_tag="ACHGT5_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7218641.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2549..3970)
                     /gene="gndA"
                     /locus_tag="ACHGT5_002709"
     CDS             complement(2549..3970)
                     /gene="gndA"
                     /locus_tag="ACHGT5_002709"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN7218642.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          857 c          693 g         1392 t
ORIGIN      
        1 tattgttttt tcgttcatct aaaagatact tacatttaat taacggatgt tattctattt
       61 aattgttcgc tagttttttt ataatgacat ccctgaattt ctcacttgct tgtattttgt
      121 accttattct ttgttggata tataaaaaaa caagtaaagc acatgcttta cttgtcttct
      181 gcctttgcga taggaataaa gattctaaaa attgtccctt gccctactac actttctgct
      241 aagatacgtc ctttatagtt ttcaaccagt tgtttagcga tagacaaccc cagaccattt
      301 ccacccttcg ttctagctct tgctttatcc actcgataaa aacgattgaa gatcttatta
      361 aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg cgatctcaaa ttcattcaga
      421 gtggaagaaa tagagatatg gacttctttt cgtgtagttg aatactttac tgcgttatcc
      481 aatatgataa tgattaattg ttcaaagtga ttacgataaa tttgcagggt cacttctttt
      541 gtcaaatcat catctaaagt gatcacatag tctggatata atatcttgaa attattataa
      601 acttgatagg tgacttcttt tcctttagtt gtttcattcg cataatgaac atctacttgt
      661 tctgctcttg ataagtctag catctcttga actaagcttt tcatacggct gatttcttgc
      721 atgctggcgc ttaatgattc ttctaatatc tcaggatcat ctttccccca acgatttagc
      781 agactaagat gcccttcaat aattgcaaca ggtgtcctta actcatgaga tacgtcttcc
      841 acgaattgct cttgttgttc aatatacaaa cgcatacggt caagcatctc attgaatatt
      901 tcagctaaat ctgccaattc atcatttgtg tcgatttcag gcatgtggat atctgattgc
      961 ggatctttac gtatagtatc cattgtatct ctgagtacct tgagtggttt taagaaataa
     1021 gaagataaaa agaaacctaa aatactactt aagatcaaag aaacaacttc taaaacaata
     1081 agagtcaata atagcttatt tcgaatatca tagaaagaag ataattcata gaagctttgc
     1141 gcatagccga tcttttctct tgtaccttta gaatatatag gctggataga taaaaagcca
     1201 gtttttcctt ctagtgtaac gatcgttgga actttacgtg tagtctgaat caggtttcga
     1261 ctgttttcat gggttttaaa aatcaagttc tcgtccaaat cataaacata aagatccatt
     1321 gatggctgcc ctagctcagc tataaaggaa tcgatcttca ttaagtttcc ttcgaccagt
     1381 gaagtatgat ctacgacctc atctccgtca tttgtcgctt tcgtcagatt acgatacgta
     1441 tttactaatg tcagttcttc atccgagtta gccaaacgag aagctacttc tgaaatcgta
     1501 cgctctacat tgtttctttc ttttgccaca atcagattga tagatgattt ataggtaatg
     1561 acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac tcgcaaaggc ccacttgatt
     1621 gtcaaggagg gccctttcag ttctcttttt tccgattttt tcggctttat cacgagcgca
     1681 tcacataacc tgttccgcgt acagtttgga tatagctttc ctccccggga acgtcaattt
     1741 tgttgcgaag ataacgaata tatacgtcca ctacgtttgt ttcaacttct gtttcgtatc
     1801 cccacacttt atttagtaaa acgtcacgtg ctaataccac gttcacgttt tccattaaag
     1861 taagcagcaa ctcgtattct ctttttgtta gttcaattac ttcatttccg cgacgtacga
     1921 cacggttttc tttttcgata gtcaaatcgc gataagtaat agtagtttgt ttagccacat
     1981 ttttatcgcc ttcaatatcg attcgacgca acaatgcacg aagacgtgct aataattctt
     2041 cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg gtcaagtcca gatacacgat
     2101 cgatcacaga atctcttgca gtcatcataa tgattggtgt atttttgact tggcgtacac
     2161 gtctgcagac ttctaaaccg ttcaattctg ggagcatcag atccaacaat atggcatccc
     2221 actcattatt taatgccgca tctaaaccag ttcggccgtt gtagtggact tctgcgttat
     2281 acccttcatg cttcaactct agctcgacaa atcgtgccaa gttcttttca tcttcaatga
     2341 ttagaatatt gctcatttat tattttatcc tttctattct actggctttt taaaaactct
     2401 atcattatac cgataatatg cttaaaaatc tatagtcatt ttatcatgga tagcaatttt
     2461 aaaaaagagc tgaagtctac tcataacaat cgacttcagc tctttcgtcc tctttttatt
     2521 ttctcatttc ctaacagccg aaagactatt attcttcatg gtaccatgag taatggaaga
     2581 ttccttcttt gtcgacacgc tcataggtat gtgcgccaaa gtaatcgcgt tgggcttgga
     2641 ttatatttgc aggcagacgt tctgctctgt atgaatcgaa gtaagcgatg gctgatgaga
     2701 aagtcggtac aggtacgcca gcttggacag ccaaggcaac gacgtctctg actgattgct
     2761 gatatttttt cgtgatttca acgaagtaat catctagcaa taaattttcg atctcaggat
     2821 ttttgtcata ggcatccgta attttttgca agaatcgagc tcggatgata catccagcgc
     2881 gccagatttt tgcgatttca ccaaatggca agtcccaatc aaattctttt gaagcgacac
     2941 gtaattgagc aaatccttgg gcataactca tgattttact gaaatacaac gcttctcgga
     3001 ttttttcgac caattctttt ttgtctcccg tgaactcaaa atcgttcgta cgagacaaaa
     3061 tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat atagcgggca aatacagatt
     3121 cagtgatcaa tggaagtggc acacctaagt caagcgcgct ttggcttgtc catttacctg
     3181 ttcctttatt cccagctgca tcaaggatca catctactac tggtttacct gttccttcat
     3241 cgtctttacg tgtcagaatg tcagcagtga tttctatcaa atagctgtct aattcgcctt
     3301 gattccattc tttaaagata tcagccattt cttctactga aaggtttaaa atattcttca
     3361 ttaagtcata agattcagca atcagttgca tatctccata ctcaataccg ttgtgtacca
     3421 ttttcacata atgtccggct ccatttgggc caatatatgt gacacatggt gctccgtctt
     3481 ctgcttttgc agaaatttgc tctaagattg gtgcaactag cgcataggct tctttttgtc
     3541 caccaggcat gattgaaggt ccttttaaag ctccttcttc accaccggaa acgcctgtac
     3601 cgataaagtt gatgccagag ttggctaact cttcattacg acgcatcgta tctttaaaga
     3661 atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag gtgaggaagt aactcttgga
     3721 ttgttgcatc tgttgccggt cctgctttga ccattagcaa gatccgacgt ggtttctcga
     3781 tagattctac aaattcttcg attgtatatg ttgctttgaa atttttatct gggtgttctt
     3841 ctactacagc ggttgtttta gcccctgtac gattgaataa agccactgaa tagccgcggc
     3901 tttctatgtt caaggctaga tttttaccca taacggccat accgacaacg ccgatttgtt
     3961 gttttgacat atgaactcct cctacatacg ggatttctcc ctaatctctt acattatagc
     4021 ttaacacggc tacattgcaa actaaagaac aacatatt
//
LOCUS       DAZBWR010000166         4040 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000166 DAZBWR010000000
VERSION     DAZBWR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.171"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..705
                     /locus_tag="ACHGT5_002710"
     CDS             <1..705
                     /locus_tag="ACHGT5_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN7218643.1"
                     /translation="NLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGII
                     PSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLK
                     VRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRE
                     SFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVS
                     PKQYQLGLVDNFNNIS"
     gene            802..1155
                     /locus_tag="ACHGT5_002711"
     CDS             802..1155
                     /locus_tag="ACHGT5_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218644.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            1772..>4040
                     /gene="esp"
                     /locus_tag="ACHGT5_002712"
     CDS             1772..>4040
                     /gene="esp"
                     /locus_tag="ACHGT5_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN7218645.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1479 a          551 c          765 g         1245 t
ORIGIN      
        1 aatctgttat cacaaaatta cgatccagaa atatatctgt ttctttatga aaataaaata
       61 ctggaatatg ttaaaaatgg agatattcga aatttagaaa acatggtttt taatctgagt
      121 aatggaataa ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa ttattctatc
      181 attgtttttg aaaagttagc acaaacgtct ataacattag gaatggatat aatagaagcg
      241 tatcaaagta gagatgcttt gatacaagaa aacgaattag ctgtatcgtt acctgaggtc
      301 ttaaaagtga gggattctgg gattgtttat tatactaagg aaatcggaaa aactaagata
      361 gagcatcttt ctcccttaat ttcttctgtc gttcaattca tcggcttaaa tatctataaa
      421 agaattactg taaaggaaat agctaattat ttttctgtaa gtgaaactaa attaagagaa
      481 agctttaaga atgaaatgca tattacgatt tataattata tctctaagcg aaaaatttct
      541 acggctaaaa taatgttgaa atcaaaccat actatcagtg aagtttcttt aggtttagga
      601 ttttcagatt cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt atctcctaag
      661 caatatcaat taggtttagt ggataatttc aacaacatta gctgagaggt tttattttat
      721 tatgagacta tatcatttac gtagtttgga ggagttgagg aaattggatt ttaacaaaaa
      781 attttttggg aaatttctga tttgagttgt ttcgtagaat ctgagcgtct aagtaatggt
      841 aataatagtg tgttaaatga atatgacaaa gtagtaatta ataaagtaaa aagtttatgt
      901 gaagaagcta gagaatctat cagtcatatt aatggtagca attttaagca aacattattt
      961 aatctgatta agctagatgc taaaatatca agttatttat tctttttaat acatgatgaa
     1021 tttatggatt atcaaaaatt ggatcagtta attgaaagag aaagctggga agattattac
     1081 gtgggtctaa ctttttcaga aaagttaaac aactacaagt taatagatta taagtatttg
     1141 agtgaaagca attaattatt catgtagagt actagacact ttaaatatgg acaattcaat
     1201 tataaagaaa aagaatcaat aatttaatga ggctgtgaca aatgtcattt tttgtttcgc
     1261 taaaaacgag tgaaaaaatg ttgataactc caatcattcg tttaagattc taggaatcat
     1321 tcaggaatgc tgtgaaaccg atactttata attataaaga aggtgatttc aaattccgaa
     1381 gaaggaattt gaagtcacct ttttacttat gtcacagtct cattcagtaa tgcgggataa
     1441 ggctccttat tccgctattt gaatactctg aagtttaatc taagaaagag taatattttg
     1501 aaaacatgct tcatcgcata gaaatgaact taatttcaga aagaatacat acatatcttt
     1561 ttatatgttt tcagctgctg aatggttaaa aagcacaaat atccgaaaaa aacatgaaga
     1621 aatgtacttt ttttgttgta atgtgcatga aattctttaa gtaaactagt taacataagt
     1681 ttagtaagat tcctattctg tgaataatac gtatcagatt tatcttttat tcttactaag
     1741 aaaataacta ttaagggagt tgatttgaat aatggttagc aagaataata agagagtatt
     1801 tctagaaaaa acaaagaaac gagtattgaa gtacagtatc aaaaaattaa gtgttggggt
     1861 tgcatctgtt ttggttggtg ttggtcttgt cttgggaaca actgaactag ttcaagcaca
     1921 agatgaaatt tctccaagca ccccacttga aacagcgata agctccgtac aagtaggtga
     1981 caaagtagct tcggggaata cttttcagga gaatcctggt tatacgaaaa attataactt
     2041 ttcggattta caatttagtc ctcaagaact aactggagac acactaaaag gcaatacaat
     2101 tggatttgaa gtttacggaa aacataatat tgcagcatca actaaaaact gggaaatccg
     2161 tcttcaatta gatgaacgat tagctaaata tgtagaaaaa attcaggtcg atccaaagaa
     2221 aggaattgga tcttctagac ggacctttgt aagaattaat gattcgctgg gtagacctac
     2281 aaatatttgg aaggtcaatt atattcgtgc aagtgatgga ttatttgctg gggctgaaac
     2341 aactgataca caaactgctc ctaatggggt aattacgttt gaaaagagct tagatgaaat
     2401 ttttaaagag ataggcatag acaatctcaa gaccgatcgt ttaatgtatc gaatctactt
     2461 ggtaagtcat caagacgatg ataaaattgt accaggtatt gatagtacgg gctacttttt
     2521 aacagattca gatgatttct ataatagctt agatgtatct gaaaataatc ctgaccagtt
     2581 taaacatgga tcagtaagtg caaaatatga ggaacctaat actcaaacaa aagatggttc
     2641 aggatccaca ggtgctaatg gagctataat cttagatcat aagttaacga aaaattataa
     2701 tttctcatat tcagcttccg caaagggcac gccttggtat gctaattaca aaattgatga
     2761 acgattagtt ccatatgtag cgggtataca gatgcacatg gttcaggctg acaaagtaac
     2821 atatgatgtt tcttttgaat cagggaaaaa agtggctgat ttagcaattg aacggcgcaa
     2881 agatcatgaa aattatggtg taggttcaat tactgataat gatcttacca aacttattga
     2941 ttttgctaat gcaagtccac gtccagtcgt tattcgatat gttttacaat tgactaagcc
     3001 attagatgaa atcttagaag atatgaaagc aacagcacaa gttgaagaaa ataaaccatt
     3061 tggtgaagat ttcatctttg attcttggtt gtcggatacg aataaaaaat taatccagaa
     3121 cacttatgga acaggttatt attatttgca agatattgat ggtgatggaa accctgacga
     3181 taaagaagag agcggagaca cgaatccata tatcgggaaa cctgaattag aagaagtata
     3241 tgatgttgac acaacagtta aggggaaagt attcatccac gagttagcgg gaacaggtca
     3301 caaagcccaa cttgttgata aagaaggtac tgtattagca gaaaaaacta tcgctccaaa
     3361 tgaaaaagat ggggctccaa tttcagatac tgtagaattt gaatttacgg gtgtagattc
     3421 aagtaaatta atcgcgaaag atgaattaaa aatccaaatc gtttctccag gttttgataa
     3481 accagaagaa ggttcaaccg ttattaagga atcaccaaaa gcggttgata aacaaaccgt
     3541 ggtagttgga tttaaaccag atgctaaaga atcaattcgg aataataaaa acttacctga
     3601 agatgcagag tattcatgga aaacagagcc tgatacttct aacgttactg atagtacgaa
     3661 aggtattgta actgttaaga tcggaaatcg aactttcgac gtggatgtag agtttgctgt
     3721 aaaagcttct caagctatgg aaaatgatgc aacatacgta cctataacaa caactccaga
     3781 aacgacagtt caaagtggaa aacctacatt tgataaacca gatgttcctt tagctaaaga
     3841 tgccttttca attttagatg tctataataa ggacttcggt aatgcaagtg ttgatgcaaa
     3901 tactggtatt gttacattca ctccagctaa aggtgtagga gaatcggagc cgattactgg
     3961 aacaattcct attaaaattg tttaccaaga tggttctgta ggcacgaccg atttagcagt
     4021 aactgtaagt aaagatattt
//
LOCUS       DAZBWR010000167         3931 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000167 DAZBWR010000000
VERSION     DAZBWR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.172"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHGT5_002713"
     CDS             <1..156
                     /locus_tag="ACHGT5_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN7218646.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHGT5_002714"
     CDS             175..1452
                     /locus_tag="ACHGT5_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7218647.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACHGT5_002715"
     CDS             1462..2118
                     /locus_tag="ACHGT5_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN7218648.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="ACHGT5_002716"
     CDS             2118..3494
                     /locus_tag="ACHGT5_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7218649.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3931
                     /locus_tag="ACHGT5_002717"
     CDS             3624..>3931
                     /locus_tag="ACHGT5_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218650.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     "
BASE COUNT         1585 a          418 c          688 g         1240 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta a
//
LOCUS       DAZBWR010000168         3720 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000168 DAZBWR010000000
VERSION     DAZBWR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.173"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..355)
                     /locus_tag="ACHGT5_002718"
     CDS             complement(<1..355)
                     /locus_tag="ACHGT5_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218651.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="ACHGT5_002719"
     CDS             complement(615..1163)
                     /locus_tag="ACHGT5_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218652.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="ACHGT5_002720"
     CDS             complement(1164..2021)
                     /locus_tag="ACHGT5_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN7218653.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="ACHGT5_002721"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="ACHGT5_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="ACHGT5_002722"
     CDS             complement(2583..2912)
                     /locus_tag="ACHGT5_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN7218654.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="ACHGT5_002723"
     CDS             complement(2989..3261)
                     /locus_tag="ACHGT5_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN7218655.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="ACHGT5_002724"
     CDS             complement(3261..3524)
                     /locus_tag="ACHGT5_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN7218656.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1401 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
//
LOCUS       DAZBWR010000169         3719 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000169 DAZBWR010000000
VERSION     DAZBWR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.174"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHGT5_002725"
     CDS             85..1434
                     /locus_tag="ACHGT5_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN7218657.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHGT5_002726"
     CDS             complement(1562..2152)
                     /locus_tag="ACHGT5_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN7218658.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHGT5_002727"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHGT5_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN7218659.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1050 a          691 c          733 g         1245 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctca
//
LOCUS       DAZBWR010000170         3703 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000170 DAZBWR010000000
VERSION     DAZBWR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.175"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..311
                     /locus_tag="ACHGT5_002728"
     CDS             <1..311
                     /locus_tag="ACHGT5_002728"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN7218660.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            338..637
                     /locus_tag="ACHGT5_002729"
     CDS             338..637
                     /locus_tag="ACHGT5_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFN7218661.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            811..1356
                     /locus_tag="ACHGT5_002730"
     CDS             811..1356
                     /locus_tag="ACHGT5_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFN7218662.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            1512..1808
                     /locus_tag="ACHGT5_002731"
     CDS             1512..1808
                     /locus_tag="ACHGT5_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218663.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            1801..2691
                     /locus_tag="ACHGT5_002732"
     CDS             1801..2691
                     /locus_tag="ACHGT5_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFN7218664.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            2773..>3703
                     /locus_tag="ACHGT5_002733"
     CDS             2773..>3703
                     /locus_tag="ACHGT5_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN7218665.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNG"
BASE COUNT         1368 a          546 c          735 g         1054 t
ORIGIN      
        1 aaatgtttca aaataaagga caacgttatg tcacaaaggg agtaatggac agtttactgg
       61 tggaattaca aactttatgt tggaatttaa tcgatcaaaa cgttcaaaag aagcttcctt
      121 tagactatct tcaaatcttt gaattttcga tagaaaaagg aaatcaaaag ttggttcatc
      181 gtcaagaaga accggaagca agaaaagagt atctgatttc acctaagctt cgtttggaat
      241 cagtgtctca aaaaatatgg gtcattgatt cgggtgagta tcaaacgatg ttattaccag
      301 aagaatactg atatctaaaa gaaaggagcg aaatgacttg ttagaaacgg ctatcgaagt
      361 actggaaaag tgtgcacagc tagtcactac tcctgaagag tggggctatg aatccgttac
      421 gatggaaaaa gaggaaattg aaatgggtac gcttcctaaa aatgttcatc taccacgttt
      481 agtgatgaca catctttata tttattgcgc accagaagat ggaaaagact atgtagttta
      541 tttcattaca gatattacca gccagcgaga atttgtttgt gggttactgg tagaaggacg
      601 attggtttgg agtcagattg gaggaacgaa tgaataagat cgattatttg agaatgattg
      661 cgaaatgttt agaagatatc cttaccgcga tttatcgaat tctgaagaaa aaaggagaaa
      721 gaatatgaaa tattttcaac tagtattgac acttataacg gcaatggtaa ttgaattgct
      781 tgaacaaaaa ttagcatagg agaaaaagag atgattgaaa aagaattagt gaccacacaa
      841 acagaagttt ggcgagatcc tcatcatacc catcgctatg ttttcaaacg acaatgggca
      901 atcaaaggaa aagaagagaa agaaaaatta gcagtagtca ttaccattcg tccaacagat
      961 acagaaccat ttacgaatga tttgagtatg ttattaatcg aaaagaatat ccgacaattg
     1021 ggatttactg gttttattgc cgtgaatctt ttttcgtata caaaagcgaa aagtcctcgg
     1081 gtattttctc gtggcaatga tgaacaatct ttggaaatat taacgaccat ttttacagaa
     1141 aagaaaatca acactattat ttttgcttgt ggttcgatta cggcaacgag tcaagtggct
     1201 tatgaacaag caaagaccat ttacgatcaa ttgagtgcga aacaaaagaa gatgaccaaa
     1261 gtattgataa atgcagaagg caagttatct cacccattaa gtattcatgt tcgtaatgaa
     1321 tggcaattgt cggatcattc tttgttattt caataaacga aaataagtat tcaatgtata
     1381 tgtttctttt tctgttattt tacttgttct tttgcagact aaacaacttg taaaacctct
     1441 tgttatatgc attatcaatg aaggaatcaa tatgatattt ttaaaatgtc aggggaaatg
     1501 gaggtgagca agtgaaagta gatggggaat gtttgacgag ttcgtataaa agtatctatg
     1561 aattttttga ggaaaaagga ctagacacgg atttacttta tcagttttat atcgcttttc
     1621 gtgggcaaca gttgtcgttt ccaatgaaaa tgtatgaccg agaattagtc gctcaaaaaa
     1681 ttgaaacaat gatacaagaa aaccattcca ttgattctag gaaattaacg gatcggtatg
     1741 gtttttcaac gagatggatt caaagtatta tcagacaaca acaaaaggag caacatgtac
     1801 atggctaatg aagaaacagt aggtttgaga atcatagaag aagcattcaa attaccaatg
     1861 gtcaaagttg atcgtcgagc atttttgatt caaacatttc aagggaatgt ttctgatatt
     1921 gaaggactca tagaaaaggg tccacaagca tttttatcca aagaaatgct agatcgtgaa
     1981 gcacaaaaat gtattcaatc gattaccttg aaatcaagtg cggtatcgtt agcaactgga
     2041 ctgccaggag gtatcgctat ggcagcaact attccagcgg atctccttca gttctttggt
     2101 tattcattac gattagcaca ggaaatttcg tatatttatg gatttaagga tttaatggga
     2161 gagactggag agttcagtga agaagcaaag aatacgctga tattgtattt gggaattatg
     2221 cttggagtaa cttctgcagg ttctgccgta cgagcgatgt ctggaaagct gtctacacaa
     2281 gcattgaaaa agattcctca aaaagcattg acacaaacgc tgtattatcc agtaatcaaa
     2341 cgagtactag gtatttttgg tacaaagtta actaaaaata cctttgcgaa aggtatttct
     2401 aaagcagtac cagttgtggg aggcgttgtt tcaggaggca tcaattactt gtctatgaaa
     2461 ccaatggcaa cgaagcttca aacagagtta gggaaaaatg tagcgtatac agaaaaaaat
     2521 ctccaacaag atttagatat tttagaaggt gaatttgaag aagtatcttc tgatatcgtg
     2581 tcttctgata cagttctttc tcaattagaa agactttctc acttgttgga aacgaatatg
     2641 attacagaag aagagtttca acaattgaag caagagctgt taaagaaata attatttaga
     2701 aaaagcaacc acttatacgg gtggttcttt ttcttgaatg aattcaaatt tattattagg
     2761 agaagaaaaa ggatgaaagc aaaacgatta ttcctattag gcacgattct tttacttgga
     2821 ggatgttcta ctgtctctca aaatgaacaa aagaaacaag tggattcaac aacttatgaa
     2881 actgaaacaa caagaaagga atcttctaca aaagaaatag aaacaactac tcaagaaact
     2941 aaaacaacag agtcttctag taaggcaacg agttcagata cgagtgattt tcaaataaaa
     3001 caagaattat gggatacaaa taaagcatct caattagcaa cttttgtccc acaatgggga
     3061 aagacattag gtcaagaata taaaagctac aatcctcaga acaatgtaag tctgtatggt
     3121 acgcctttac caagtgcagt tattgatgga aattggaaaa tggcaatcaa tgaagcacca
     3181 gttactgtac aatggtcaga agatggaact ggaaatgcag gatttaattt agtagcagtt
     3241 tattctgatg tagaaactgg agaatattta gggcagcatg tttacttttt cggttttcaa
     3301 aatggacaac ctaaagttta tttaacgcaa caaaatcaag gtaatgaaaa taattatctg
     3361 tactttaacg aaactcaaaa tcaacagcta aaacaaggat ttacagatat cattaatgga
     3421 caaactcccc aaacaccagt agtagaagaa tctactcaac aagcaacaac tacaacgaca
     3481 caatctacaa gtagttcagg aatttcaaat tgggaagctg ctaaagaaac tgtactaaat
     3541 aataaagcgt tatggattta tgagtctcca gataaaaatg aagcaaatac taatattatt
     3601 gaatccaccc aatatagaaa agatttaatg caagatgaaa atggagaatt ttatccagtt
     3661 gatatttcta ctgatgatat tggaaatacg gtaatgaatg gag
//
LOCUS       DAZBWR010000171         3697 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000171 DAZBWR010000000
VERSION     DAZBWR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.176"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..1189
                     /locus_tag="ACHGT5_002734"
     CDS             23..1189
                     /locus_tag="ACHGT5_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN7218666.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            1235..2731
                     /locus_tag="ACHGT5_002735"
     CDS             1235..2731
                     /locus_tag="ACHGT5_002735"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN7218667.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            2812..3495
                     /locus_tag="ACHGT5_002736"
     CDS             2812..3495
                     /locus_tag="ACHGT5_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN7218668.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            3504..>3697
                     /locus_tag="ACHGT5_002737"
     CDS             3504..>3697
                     /locus_tag="ACHGT5_002737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218669.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         1264 a          641 c          792 g         1000 t
ORIGIN      
        1 tttataaata aggagattgt gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc
       61 ggcaagcggg ctagaagcac tggttggtaa agaattaaga gatcttggaa tcgaatgcca
      121 agttgagaac ggacgtgcac gttttgaagg aacaatggaa acgatcgcta cagctaattt
      181 atggttacgt acagcagatc gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt
      241 tgatgaattg tttgaaaacg taaaagcatt gccttgggaa gattttcttc cactagatgc
      301 agcttttcct gtagctggaa aatcgatcaa gtcgaaacta tatagcacac ctgattgcca
      361 agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc tattatcacc gtccagcttc
      421 tgtaccactg acggaaactg gcgcacattt tcagttggaa gtagctttgc tgaaagacca
      481 tgtgatggta actctggata cgacaggacc aagtttattc aaacgtggtt atcgcttaga
      541 aaaaggtgga gcaccattga aagaaaacat ggcagcagca cttattatgt tgacaaattg
      601 gcgaaaagac cgtccatttt atgatcctgt atgtggttca ggtacgcttt gtattgaagc
      661 agccttaata ggccataata tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg
      721 ggattggttc agtcaagaaa tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga
      781 ttatgatatt gaattagata ttactggttc agatatcaat ggacggatga ttgaaattgc
      841 caaagccaat gcagaagaaa tcggattagg ctcttcgatt acctttaagc aacaagcagt
      901 caaagacttc aaaacggata aagaatacgg ggtcatcgtg gctaatccgc cttacggaga
      961 acgtctagga gaagaagaaa ctgttcggaa attatataaa gaaatgggcg atgtattccg
     1021 tccattaaaa acatggagta aatatatctt gactagcgac ttagcgtttg aggaatttta
     1081 cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt gcattgcgta cggatctatt
     1141 ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa gacgaataat cttactaatt
     1201 gattcattca attggatgtt tggaggaata gggaatgaaa gaaaaagagt ttttacaaga
     1261 agtaaaagaa atcaatcttc tacaacaagc tttaggaatc ctcgattggg atacacaaac
     1321 aggtatgcct gaaaaagcta gtgcataccg aagtgaagta gacagctatc tttacagcat
     1381 ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc attcgctatt tttctgaaca
     1441 tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc aaagtaaaag aagagtatga
     1501 tttggaacat cgcgtaccgg aagatcttat gagaaaatat acagctgccg tttcttcagc
     1561 acacggacaa tggcaaaaag ctagagaaac acaacaattt tctgattttc aacacgcatt
     1621 gaatcaaaat attgaattaa caaaacagtt gataccttac tggagaaaag atgaaaagac
     1681 gaattatgat gttttgctga atcaatacga acccgggatg actgtcgaaa tccttgataa
     1741 ggtatttgat cagcttaaag aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg
     1801 gactgaacct gaaacagact ttttaaatcg gagaatgacg aaaaaacaac agaagagatt
     1861 tgtagtgaaa gtaatcgaac aacttggtta tgattttagt cgtggtcgtt tagatgacac
     1921 tgtccaccca tttatgactg gaattaatcc gaatgatgca cgaatcacga cacgatggaa
     1981 cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac gaggctggtc atggtatgta
     2041 tgaacaagac atcgatgata aatatgcgta tacaccggtt tatgaaggta catcaatggg
     2101 tatccatgaa tcacaatcgt tgtttaacga gatcattatt ggaagtagtc gcagcttctg
     2161 gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga acattcgatg atattccttt
     2221 tgacaaattt tatcgttcat tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga
     2281 cagtttgact taccctctcc atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa
     2341 tgaaggggta gatgttgctg aactacctga aatatggaat caaaaatatg aagagtatct
     2401 gggaatacga ccaacaaatg atgtggaagg catcctacaa gacgttcatt ggtcaggtgc
     2461 aagctttggt tattttccct cttatgcatt agggtacatg tatgcggcac aattacttca
     2521 ttctttgcag caagaaatag atgtggaaaa tgttctagcg tcagacgatt attctccaat
     2581 caaaaactgg atgtcagagc atatccatca atatggtgca tctagaaaac caaatcaatt
     2641 aatcatggat gcaacacatg aaccgcttaa tccgcaatac ctgatcgact atatgaaaga
     2701 cctgtatttt aatgtatatc aggtaaaata aaatacaatt tacaaagata gctgaaattc
     2761 atctaatcta tagatgtttt cagctattta cttaagaaag aagtggaacg aatggataaa
     2821 ttacaagtga tcgctgatta tagtatggac caattagcgc atgatcaaac aggacatggg
     2881 agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag
     2941 gcagaccgct ttgtgacgct tgcagcagct tacctacatg atacgattga tgataaagtc
     3001 gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat tccttcgctc tttgccaata
     3061 gtagaggaac agatctcgat gattttttcc attatcgaaa atatgtcctt ttctaaaaat
     3121 ctctctgaag cggccgaact atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc
     3181 gaagcattag gagcaatcgg cattttgcgt actgcttatt tcggcggtgg acatgggcac
     3241 ccgatttttg atcctgaact gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa
     3301 ggcacgacag tgatcaatca tttttatgaa aaactgttct tgctgtctga taaaatgaat
     3361 actgattacg gatatgaaga agccaaacga agagaaacct tcatgcgtga attcttagaa
     3421 gaattttttc tggaatggtc tattgacgat gaaaatttta ctccagacaa ttggatcaaa
     3481 aataacaaaa aatgatggag gttgtgacag aagtggtcag ctccaagata tttcggatca
     3541 ttttccgtaa aagttacttc tggaacactg tttaccaaga ggtcgtgagc ctgccgttca
     3601 gctccgagtc acaaggaaca atttcaaaaa atagcttctc gtgtttgatg aatattgtaa
     3661 cttgtgattc ggaattggca ggagaacacc gtttatt
//
LOCUS       DAZBWR010000172         3680 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000172 DAZBWR010000000
VERSION     DAZBWR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.177"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            429..1469
                     /locus_tag="ACHGT5_002738"
     CDS             429..1469
                     /locus_tag="ACHGT5_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN7218670.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2293..2625
                     /gene="traE"
                     /locus_tag="ACHGT5_002739"
     CDS             2293..2625
                     /gene="traE"
                     /locus_tag="ACHGT5_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN7218671.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3244..3585
                     /locus_tag="ACHGT5_002740"
     CDS             3244..3585
                     /locus_tag="ACHGT5_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN7218672.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1356 a          482 c          679 g         1163 t
ORIGIN      
        1 attgtatttt ttagtagtat ataatctatt gttttctagc ctaaagcctt gtcttataag
       61 aataatataa tatatactac tgaaataagg tagtatatat tactagtaat ggtagtatgt
      121 tttatatgca taaggtagtt cttaatttat tttttgtttt gacactggta gtacttaatt
      181 atctttaatt ttaaaactaa attttattca tacgtttttc tggtagtatt taattataaa
      241 taatatttat ttaaagtagt tcttaatgac tttaaaggta gtacataatt cattttattt
      301 ctgattttag gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat
      361 ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag
      421 gatctacaat gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt
      481 atcaatttcc aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta
      541 agattgccta tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg
      601 atgaagaagg taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact
      661 gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac
      721 aaaagcagat gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag
      781 cagaattaaa agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata
      841 gggaaaacgt tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag
      901 acgctgaatc ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata
      961 aatcagaggg tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac
     1021 acaatataga cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga
     1081 caattatgac aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa
     1141 aaacattttc ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa
     1201 agaaaaaagc tgaaatagaa agtgatattt cgatagtttt tgaagaactc gatagttatt
     1261 atgtcaatgc agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa
     1321 ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg
     1381 aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt gaattatgca agctccccaa
     1441 gcactattac gaaagactgg ttagagtgaa actttactga caaagtgatt gttgcttcat
     1501 ggagtaacat taaaattaaa aattttttat tattctaatt atattacgaa tattacaaaa
     1561 taatagctat tttaatagaa tttataagtt aaaatattat gataaaaatc acaaacgtag
     1621 ccttttacgt cataagtgtt aaaagaaata attaatttca aatatagaaa agggaacgta
     1681 tgtttgcata ctcctttttt ttgagttata ataatacgcg aagagagata atttcattat
     1741 ttaaacaagc taaaaatctt gatattactt cttttcaggc aatacgaata ttattcgctt
     1801 acattttttg tataaattta attgtctgct atgcttgtga tgaattgatt ttcgttttgc
     1861 acttttggat gataaatttg gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact
     1921 cggaaggctg gtaaccttcg tcagcgttca tacacggcat taagatttgg cccttaatgc
     1981 gttaaccttt cagtgttacg gtattacccg caatggattt agtataacag atattcaaaa
     2041 gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta
     2101 tgcctaagca taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga
     2161 aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat
     2221 caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagaaattgg
     2281 gtggtttatt ttatgtcata tacaggaatt ttgagtctca aagatatttg tcactacggt
     2341 aaacggtgta ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc
     2401 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat
     2461 tacattggga aacaaaagca aataatttta aaagatccta tccccttaaa agagctatat
     2521 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
     2581 gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg gctgaattga tcattctcat
     2641 aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact
     2701 tagagtcact agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg
     2761 acggggtagg ttttaacgat tggtaacgta aaaaattggt caatcgagta aatgcgcttg
     2821 tttggatagt ggtgaaatga atttgtgtaa ataataagcg cagagtaaac aagagggaat
     2881 ttagaagtta gacaagttag aatagcgtct agattaatca tgagcagcta ctaagggatc
     2941 tagactttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca
     3001 caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgattattgg
     3061 atcaatcgag catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt
     3121 cgaaattttt tatgtatttt taagtaaacg aaaaacgata aaatatgaac aagtcctgaa
     3181 taaagtgaaa aaattgatat aaaacataga gaaaatgaaa tttacgtaat ggaggaatga
     3241 caaatgaaaa attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc
     3301 caacgttggg attacaaagg atttgtgacg tttagaaggg gcggaaattg taaaggatta
     3361 gatgttttag cattagacga ggatgattta tacgatcaaa cattgactga taatccaatt
     3421 ggattcggct tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg
     3481 aatgataatg gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt
     3541 gtcagctttg aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa
     3601 attttacaga taaaggctta gaaggagtca cgacgattaa taaaaaggta gtattacctt
     3661 aggcattgag taaggaattt
//
LOCUS       DAZBWR010000173         3584 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000173 DAZBWR010000000
VERSION     DAZBWR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.178"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHGT5_002741"
     CDS             186..2855
                     /locus_tag="ACHGT5_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN7218673.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..>3584)
                     /locus_tag="ACHGT5_002742"
     CDS             complement(2946..>3584)
                     /locus_tag="ACHGT5_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN7218674.1"
                     /translation="EYSDFFLFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSR
                     IGLTKAMHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMV
                     LIGMPGIEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDY
                     EAITTIIKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
BASE COUNT         1051 a          659 c          670 g         1204 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attc
//
LOCUS       DAZBWR010000174         3474 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000174 DAZBWR010000000
VERSION     DAZBWR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.179"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..907
                     /locus_tag="ACHGT5_002743"
     CDS             26..907
                     /locus_tag="ACHGT5_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN7218675.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            920..2530
                     /locus_tag="ACHGT5_002744"
     CDS             920..2530
                     /locus_tag="ACHGT5_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN7218676.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2607..3266)
                     /locus_tag="ACHGT5_002745"
     CDS             complement(2607..3266)
                     /locus_tag="ACHGT5_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN7218677.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT          950 a          677 c          696 g         1151 t
ORIGIN      
        1 ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac atttgaaaaa
       61 gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt caggtgaagt
      121 cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga cacttttagg
      181 gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat gggaagatag
      241 tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt ggggaaattt
      301 gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa
      361 aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa aaaactattc
      421 aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt cggatctcta
      481 tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt tccaagaaga
      541 agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata ttctcagtga
      601 agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg atgaagaagg
      661 aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa cagaagcaga
      721 gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc
      781 tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt ccaaattagg
      841 tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga gacattatga
      901 aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc ttttttctca
      961 aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca ctgctttttc
     1021 tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag ctggattata
     1081 cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag cttcgaatgc
     1141 tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt
     1201 tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag aagaacttgg
     1261 tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt tagctgtcat
     1321 cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta cgatgatttt
     1381 tttccaagcg gattcgatca ctgcagaagg tgcttggctt tttgggctat ctgtaggtag
     1441 tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc caagttcagc
     1501 cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac gagcaataac
     1561 agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt atttgaccta
     1621 tccatttaca actaacaact ggattccttt gctttatttg tttttgttca gtctaatctt
     1681 gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct atatccccga
     1741 acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt tattattcta
     1801 tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg gggcggcata
     1861 cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa tgaaacagat
     1921 gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca tggtagtctt
     1981 gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta cagaagaaac
     2041 ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac tttattggac
     2101 caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt ctggtgcact
     2161 agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat tgatgtttct
     2221 taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta gtggctttct
     2281 attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt actcctttgc
     2341 actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa cagctgtccc
     2401 tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc ttacgttatc
     2461 atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa gagacttact
     2521 agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg ggtcaaaaat
     2581 ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt atcaatgatc
     2641 cagacttctc gattctttct ttttacataa ccattctcct ctaataattt aaattttcta
     2701 gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt cattggcagg
     2761 aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc gactcgttct
     2821 tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa tttctcaacg
     2881 ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc cgagaaatct
     2941 ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa agactcattt
     3001 ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac tcgtaaaagc
     3061 tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc cacgatcact
     3121 aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata gtgatgtttg
     3181 acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa aggaacaaat
     3241 gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct atttctaata
     3301 aagaaacata acttccctta ttccaactga taattttctt ttatcatagc ataaacgtca
     3361 agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt tattttcctt
     3421 cttttgagac cttaagtaat tgattcctat caatttttta aatatttact tttt
//
LOCUS       DAZBWR010000175         3471 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000175 DAZBWR010000000
VERSION     DAZBWR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.180"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1316
                     /locus_tag="ACHGT5_002746"
     CDS             <1..1316
                     /locus_tag="ACHGT5_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFN7218678.1"
                     /translation="VIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPTDNWSGL
                     LTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFASILTVTG
                     FSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFGMRFPLL
                     IGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLRLFSYSI
                     VENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTTDFIANS
                     GIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSFPILVGI
                     FIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHNVSTLHG
                     GLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1282..1632
                     /locus_tag="ACHGT5_002747"
     CDS             1282..1632
                     /locus_tag="ACHGT5_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218679.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1724..2143)
                     /locus_tag="ACHGT5_002748"
     CDS             complement(1724..2143)
                     /locus_tag="ACHGT5_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN7218680.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2144..2488)
                     /locus_tag="ACHGT5_002749"
     CDS             complement(2144..2488)
                     /locus_tag="ACHGT5_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN7218681.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2838..3434
                     /locus_tag="ACHGT5_002750"
     CDS             2838..3434
                     /locus_tag="ACHGT5_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN7218682.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          900 a          638 c          630 g         1303 t
ORIGIN      
        1 acgttattta tattcatcct caaaaacgaa tcgtttcaca gaaaagaaag tatttttatc
       61 ttggttttac aggtgttttt tttcttttta ttggcttgct ttctaatact cctactgata
      121 attggagtgg attgctaact attcttacct cgccaagcaa tcttttgaca gattattttg
      181 ctttaggtgt tttcggaagc gcattcataa atgtagggat tttgacctta ctttctgtgc
      241 ttttagctta tcggcacaag gtgattttaa acggtccctt atttgcttcg attctaacag
      301 ttaccggttt ctcttttttt ggcaaaaacc tttataattc tatctcaatc attttaggtg
      361 tgtatctata cgctgtattt gtcaataaac ctttttctca gtacatcatg atcggactgt
      421 ttggtagcgt attatcaccg gtcgttagtt atatcacttt tggtatgcgt tttcctttac
      481 tgataggtat tttattgggg aatttagctg gcatagcaat tggtatactt cttccgccct
      541 tagctgctca aactttagtt ttccatcgtg gattcacact ttataatatt ggattcacct
      601 ctgggttgat tgcgatgact tttactgctg ttttgaggtt attcagctat tcaatcgtcg
      661 aaaatacatt ggttttcaat gaatatcact ttcctctcat ttggattatt tttggctttt
      721 tttcgctgac agtcggaatt ggtttctatt ataattcctt tcggctttcg gggattcgag
      781 aaattttcga ttcatctgga aaattgacaa ctgatttcat tgctaattct ggtattggtg
      841 ctactttgat caacatgggc ttagttggtc tgatgctgtc ttcttatgtt ttgctggttg
      901 gcggtcagtt aaatggacct gtcatcggtg ctattttatc tgcggtgggt ttcagtgctt
      961 ttggttgtca tttaaaaaac agttttccga tactagtcgg aatatttatt gcttctttgt
     1021 ttggaacttt tcatgagatc accagtactg gcatgcttgt tgcagctgtg tttgggacag
     1081 gtcttgcgcc cataagcggt ttttacggca gtttttacgg tgtaatagct ggtgtgctac
     1141 atattgctct tgtccataac gtcagcactt tgcacggagg actaaatcta tataacagtg
     1201 gtttttcaac tggtttcgtt gcaggtatcc ttgtaccgat actagataac tttaccgctg
     1261 tcagaaagga aaaaaagaca cttggaaaaa gaattatcaa aaagaatcat cgatgagctt
     1321 cttacttatt tatattccca tgaaataact gaaatacgta ttggagtaga atttgctgag
     1381 gaagggcttt tcatagagat acagggtacg gcagaaactc agccagaaga tttagatgta
     1441 cttgctgatt tgctgaacaa tcctcgggac cctagtatgg aaatctacta tcaagaacta
     1501 ttaggtaatc cccgacatga tcgggaagat tatcatctgt taggtagttt gattgatgac
     1561 gtggaaatca tgtatgccca gccgattatc aatattaaga tctttcgacg tgcttctcat
     1621 tcctattatt aaccctattt tctatttata aaagactgaa tcaaaaaagc agctcctgaa
     1681 agttcgatta aaatctactt tcaggagctg ttcaagcttg acattattga ttttccaatc
     1741 cttcatacac ttcatctgtt acaggttcta aaaactctga attaccagca gtgatcgcca
     1801 aatgttcaaa ccaactgtct tttgtcgcac catgccaatg tttaatccct ttgtgcgtta
     1861 cgacgacatc tccagccatt aatcgtcttg ctggctttcc ttcttcctga taccagcctt
     1921 ctcctccggt cacaagtaag atttgatagc cgtctgtatg gacatgccag tgattgcgac
     1981 aacctggttc aaaagttaca ttacctacac caacagaaac ttcgggatca ttcactaaca
     2041 tattcagata gctttgcccg gtaaatactt gagcgtaagc ttcgtttttt tcacctgctg
     2101 gaaaaatcac gccattgtgt atttcttcgt gttttgtcat catttacgcc tctttcttat
     2161 cttcgctatt ttcttctaaa tagatttctt ttgctaaact aaaagctgac caagcttttg
     2221 gccattctac ataaaaagct aaatgggtaa tgatttctgc tatttcttca ctagtaatgc
     2281 cattttgctt gccaatcttc agatgagctg gcaattgttc aaaatttcct cctgaaatta
     2341 atgcagatat ggtgatgata ctacgctgat gagcagataa ttcttctctg gaccatactt
     2401 gtccaaataa aacatcatca tttaattctg caaattttgg tgcaaaatct cctaattgat
     2461 ctcgtcctgc ggtttgtttt tctgccattt ctcttattct cccttcacga ctgttaggtg
     2521 attcaactat ggttctaagg caactttatt ttctattgtt tcttcgcatt tcattttaaa
     2581 tgtagtcttt cagcctgtcc aatgcttagt ttattcagtc gatatgcttt ttaagcatag
     2641 ttgctaacgc tttttttatt ttaaaaaaaa caagttatta aataagtctt tagttggatg
     2701 tttttttgag atcaatcttt tatacttatg atgaacttac aaaattgttt gttttctgtc
     2761 atatcaaaaa atggcgcagt tagttgcttt tctagtaaag tatagttaac aaagaacctc
     2821 taaggagttg atgaataatg aatattgctg cctctcgaaa actgataaat ttcatatcca
     2881 ttatcggatt aggcctatca atcgcgctga cgatttattt tattaattta ggtgttttca
     2941 aagatttgaa tgcgttaaga ggattagtag gtgattccat cattttagga ccgattattt
     3001 ttgtcttcat ccaaatcctg caagtagtta ttccaatcat tcctggaggg atcagtactg
     3061 cagctggtgt cctgatattt ggcccttatg ctggatttat ctataactat attggaatct
     3121 gcattggttc gattatcatt tttctgctag ggcgtcgtta tggaaagcca ttcatacttt
     3181 ccatgattag cgataagaca tataacaaat atattggctg gctggataat caaaaccgtt
     3241 ttgagaagct gtttgctcta gctatttttc ttccagttgc tcctgacgat gcgttatgtt
     3301 tgatggctgg attgacaaac atgtcagtga aacgatatac actaatcatt ttgattgcta
     3361 aaccactgtc gattttctta tatagtatgg ccttgattta tggaggtcaa tatctaagtg
     3421 gtcttcttgg ataatattta ttttgtaaaa ttagtcattg attgacaatg t
//
LOCUS       DAZBWR010000176         3444 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000176 DAZBWR010000000
VERSION     DAZBWR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.181"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2199)
                     /locus_tag="ACHGT5_002751"
     CDS             complement(<1..2199)
                     /locus_tag="ACHGT5_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFN7218683.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESLSESFNEARSSEPSITQESQIER
                     QEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVRVPRASIGLIEF
                     IRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITFEVAFFQSGGTE
                     PNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGNLGNLLIDGQDV
                     TNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREPISGGVRIAEVT
                     NPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYALEVTDVDLFNE
                     GRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNVINLNENELTYT
                     LHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTS
                     FTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLA
                     GAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFIN
                     EYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGETLQTG"
     gene            complement(2871..3139)
                     /locus_tag="ACHGT5_002752"
                     /pseudo
     CDS             complement(2871..3139)
                     /locus_tag="ACHGT5_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          968 a          764 c          544 g         1168 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tacattaaaa gactcacttg tgctttcttc tggtacactg atttctattg agaccgtatt
     1861 tgattgaata tcaccgcttc ttgcggtcaa ttcgtattta cctgaatgct gggaagaata
     1921 agtaatcaga cgttcgtaat cattgagttg cttcacttcc ccttcaatca aatcctcact
     1981 cttatcaatg accaatgcat ttagcaggtc tggtgttcct ctgactcgca actggaactg
     2041 ctcacccaaa gcagcctcct cttgatcaac ttcgatcgtc agcgaacgct ctggctgttc
     2101 tgctaaagac tcagctagaa ccgttatgtt cccaaattga ttgatcacaa taagcataac
     2161 tagaaacata cttaacagtt tatgaaattt gttcctcact attactcccc cttataattt
     2221 tgactaaaca acaaggtacc cgataatttt ctaaaaagat tccccaactc aaaatggctg
     2281 cgttacctcc ctcgtatttt cggttcaact caagcgttgc aatgtaagct gtaaacaaag
     2341 ctaatgattg ccgattgtct tctgttgatt gattcgatag ctttgtatga cttttagttg
     2401 tccgtgtggc ttaaccataa ttatgatttc acgattcctc ctgccagtgc cttgcacaag
     2461 ctggagcgta accctaatag ttattttgtt aatgtactta tatactttta tacttttcca
     2521 ttagaaacaa tcactaaatc gttgatacca aggtcagact ctagaatttc atcagtgagt
     2581 ccctgtattg gttaaccttc atcaatccca aaaaaatata tcaatgctta gcatcaaaat
     2641 gaatatatag tttagaatac ttgggattca tcgataactg atcgcttatt tggccatatt
     2701 atttggcact tggttcaact aaacgcattt aggatgttcc ttcattctcg tcatggatgt
     2761 tctcaaaccg tcattactaa taaactgtcc accgaatcga tggttcattt cttgcatttt
     2821 ccacgtccaa ttactctttt tgctctttat ttgggcgtag tttcactttc ttaacttgta
     2881 tcgtcttcca tcaactcctt aatcatcttc ttagctttat tggctcggtt tccttgtaaa
     2941 aggtatctac aaatagtgac aatctgtatt aaatcttcat cattttctgt aatgtcaata
     3001 atttctatat tctgtaccat atattgcaaa gggtttcact aagttcataa ccgaagcgaa
     3061 ttaaacaact tttgtataga ataactacct tttctatttt tccattggta attctatcta
     3121 ttaattggtt taatcctttt gttgtagttg attccactac caagatgatt ctctcgaatt
     3181 ttaaagcttg aagatgtatt gctatttttg ctaactggcg tatttgcaac tatttttccc
     3241 ttctattctt ccttcattga ttcccctcta aaattactat ccataaaaaa ttgcataaat
     3301 gacaatagta tttatattag tcatactgct ttttaacaca gcaaattcca gaaaaacatt
     3361 cgttacatat ttgtgaattt ttccaaaaaa taacattttt ataaaacgac gaaaaataaa
     3421 tcactaccag tcaacttcta taat
//
LOCUS       DAZBWR010000177         3392 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000177 DAZBWR010000000
VERSION     DAZBWR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.182"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACHGT5_002753"
     CDS             <1..790
                     /locus_tag="ACHGT5_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN7218684.1"
                     /translation="YPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRF
                     CQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLN
                     EAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVND
                     LVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGG
                     SLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1020..1250
                     /locus_tag="ACHGT5_002754"
     CDS             1020..1250
                     /locus_tag="ACHGT5_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN7218685.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1473..1571)
                     /locus_tag="ACHGT5_002755"
                     /pseudo
     CDS             complement(1473..1571)
                     /locus_tag="ACHGT5_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1836..2291)
                     /gene="tnpA"
                     /locus_tag="ACHGT5_002756"
     CDS             complement(1836..2291)
                     /gene="tnpA"
                     /locus_tag="ACHGT5_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN7218686.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2534..3392
                     /locus_tag="ACHGT5_002757"
                     /pseudo
     CDS             2534..3392
                     /locus_tag="ACHGT5_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT         1121 a          585 c          590 g         1096 t
ORIGIN      
        1 ttatccattt ttaacgaagt ctgaaaaaaa agtcgctgat tttattttaa attctgggaa
       61 aactattatt tatagtacga tgagtgatat taagaaaaac acaaaagttg gcgatgctac
      121 tattattcgc ttttgtcaaa aaattggttt ttctggtttt tctgatttaa aaattgaaat
      181 tgcaaaggaa gactttagtc aaaaaaaaga acatccgact agtgtaaagt actatgatga
      241 aattgctaat agtttaacag aagctctcta ttccactatt aggcttttag acgaagaaaa
      301 attgaatgaa gccattaaaa tgatcactca atcaaaaaac atatatatat ttggagtggg
      361 atctagtgga aatactagtc ttgatttaga aaatatgttt cttcgcgtag gagttcaagc
      421 aaaagctgta ctagaccctc attttcaagc ccaagtcgct tctttattaa cagttaatga
      481 tttagtaatc attttttctt tatctgggaa aacgaaagat acttatgatt cattgaaaat
      541 agctaaaaaa aatggcgcaa aaattattgc tataactaac tatattcatt cacctatagg
      601 aaagtctgca gacttagttt tacaaacagc cattgaagaa tttcttaatg gtggatcttt
      661 agctggaaaa atatcacaac tttatatttg tgatctatta gttcatggtt atgaacaaaa
      721 caataatatt gattcagttg aattaagaga aaaagttttg cgctctatta ttgataaaag
      781 tattgagtga actaccccca cttagctaaa cctaacggtt tttaacgatt gaagtaggca
      841 attcttggga tgagagcata cttgatagct tattttttac taatagcggt ggctcttatt
      901 gaccaaatat ccctgtagtt cctacggttc ttgattttat ctaaagatat ttttatcgtg
      961 gaagctattg atcaacttga aagaaaaacg tgtagctcat tatcactagt ttatcgagta
     1021 tggatgaagc aatggatcat cctctcttaa ataaatttat taaggatctg atccttcaaa
     1081 ttttttccat gatcgatgag caggaaccta ctgaatctaa acgacatcgg gcacaaggaa
     1141 ttattaagat tgctaagcga atgttttcta taaagggtgt tctaagctat acagtgttaa
     1201 ttcaaaagat tcccaatcat cttgtttata agaacaacgt tgaagattaa aaaagtgttt
     1261 caatgctaaa attgcaaaat acttcaatgt tatgagacaa actgtctata agaatcaatt
     1321 tccacccatt tcatcacttg ttaaaagttt atgatccttt tgatttttat attaatttat
     1381 ctgtatttaa tacagatttt atttattagt tcatctgcct attgtatttt attttgttgc
     1441 cttaccatct gaagtaaata tactcttaat tgtcaaatta agttagacag atcatcaata
     1501 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat ccagtgattt tctaggaaga
     1561 ttatttctgc gccagcattt tctaatgttt gaatttgtgt tttgtaatct tggttgcttg
     1621 tgctaattct tgaatagccg taaattgcca aaatttcatc accctttctg gacataagtt
     1681 tctgacacca gttaaaatca agtctatcag taacccatac acccgtgagg acgggtgtat
     1741 ttttaatccc cctagaaggg gtcgacactc gctgcccctt aaaaggggat ttttaaaaga
     1801 ccgccaactg ctcatcttca ctcacttact tctctttatt ttttaaatgg ttctacgtac
     1861 tctcttttac ttataatata tcgaatgcga tcatcagctt cttgttcacg gatgtatttt
     1921 tcatttgtct tttgattcaa tccgactgta ctcacatagt atctcttcga ccaaaaggat
     1981 ttgtttccgt atttatattt gagatttgcg tgctgctcgt ggattagtac cgcactacga
     2041 cctttcagat accccataaa actggatata catatttttg gtggtactcg aacaagcatg
     2101 tgaatatgat ctagcattgc atgtgcttct atgatttcca catctttcat ttcgcacaac
     2161 ttcctaagta tttttccaag atcttttcgt aattgcccat agattacttt tcgttgatat
     2221 tttggtgtaa aaataatatg atacttacaa ttccaacgag tatatgctaa acttttatcg
     2281 tcatttgaca tgaaaaaact cctttcgatc tgatgagcgg ttggtagcca ctcactagta
     2341 tatcgaaagg agtttttatc gctaaagata cttcttccac gcgtagaaac gcgcggtttt
     2401 cttgtttcgc acgctttacg cacaaaactg actgaagtca ttaagaaaat aaaagggtct
     2461 aaaactttta agttttgtac cgacccccaa aagttagacc aaaaaatcta acttttggag
     2521 gtcggtattt tttatggcta agtatacttt tgaacttaaa ttaaagatcg ttcatgatta
     2581 cttggatggt aaaggcggaa gtgattatct ggcaaagaaa tattccatta aggctccatc
     2641 acaagtgaag cgctggatca atgcctatca agaatttggt gaagagggtc tagtaagaaa
     2701 gcgacaaaag aaaaaatatt ctgttcaatt taagctggat gcgatagagt tatatctaac
     2761 aagtgagctg tcctatcgtg aagttgccaa cacacttaac atgaacaacc ctaatttgat
     2821 cgctagttgg atgcgtcaat tccgtgaggg tgggatcgat ggactctcaa aaacgaaagg
     2881 atgtccacct atcttgtcta agaaaaatga acccaaaaat aagaaaaaat ctacaactaa
     2941 agcaacgtct aaagaacgtg aacgcatcga ggagttggag aaacgagtcc gttcccttca
     3001 aattgaaaat gcctttttaa aagaattgag gaagctgcga aaacaggaag cccaacaacg
     3061 acgaatgaag caatcgcacg aatcatcgca agcctccgag gaccgtttaa attagttgag
     3121 cttctagaaa ctttaaagtt ccctaaggca atctttatgt actggcaaaa acgattggac
     3181 cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta aagttcggga gaagcacaag
     3241 gattatggct gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa
     3301 aagaaagttc agcgcctgat caagaagcta ggaataaaag taacgacata cacacgtaaa
     3361 tcacggcgct acaattctta tcgaggacaa gt
//
LOCUS       DAZBWR010000178         3390 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000178 DAZBWR010000000
VERSION     DAZBWR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.183"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..870
                     /locus_tag="ACHGT5_002758"
     CDS             70..870
                     /locus_tag="ACHGT5_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFN7218687.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            886..1764
                     /locus_tag="ACHGT5_002759"
     CDS             886..1764
                     /locus_tag="ACHGT5_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFN7218688.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1777..2757
                     /locus_tag="ACHGT5_002760"
     CDS             1777..2757
                     /locus_tag="ACHGT5_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN7218689.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3028..3120
                     /locus_tag="ACHGT5_002761"
     CDS             3028..3120
                     /locus_tag="ACHGT5_002761"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218690.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1169 a          557 c          712 g          952 t
ORIGIN      
        1 gacagataat caaaaagaat ttatagttgt taccgtagta gctactaaaa ataaagtgag
       61 gattaatcaa tggaaacgat tattcaaatc atgaaaaaat atgatttttc agagatggag
      121 acaagagtgt ataccacact attagaaaaa ggtaatctta caggatatga agtaagtaaa
      181 atctcaggtg tgccacgctc gaaagtatat aatattttgg aaaaactatt aaaaaagaat
      241 ttgattgtcg ttaataaatc agaacctaaa ctttatcatg ccatctcggc aaatgagttt
      301 ttagagaaat tagaaaaaag tgttaaaaat gatctttctt ttcttaccaa gaatctaggg
      361 atgatcaaag aaaaagatga agaagaaatg ctgtggaaag ttgatggtat cgagtatgtt
      421 ctggataaag tagagcattt ggtgaaaaac gcaaaagaaa gtttgctcat tcaagtttgg
      481 catgagaatt tcacggattc actactaaag gcattgcagc aagctgaaaa aagagttgat
      541 aagtttgtac tgattttatt cagttctacc catgaatatg atcttccctt agaaaagtat
      601 tatattcatg gttttgaaac agacaaactt gctgactttg gagcaagatg gatcaacatc
      661 gtcgccgatg agcaagaagt cgtgtttggt accattaacg aagaactgca atcaaccgat
      721 gtcacttgga caaaaaacca cgccatggtc aatctagcaa aagaatatgt caaacatgat
      781 gcatatacac taaaagtgat tgcagaatct cctgaagaac ttaaagcaaa atatggtgag
      841 ggctttgagg gaatcagaaa gatttactag taggaggaat tttttatgat tcggaatatt
      901 ttgctgacac taattgtcgc aatcaatcta tattttatta tttactttct aagagacctt
      961 ttcaaaaaca aacaaaattt caaagaagaa ccaggggata tgcgtctgtt gcccttcacg
     1021 tcatttatca tcttctttct ttcaacattt ggcgtttccg actttgctat cggaaccgtc
     1081 ctttatccaa agttgaaatg ggtcagcatg aaaaaactac ccggaacgtt gaatacccaa
     1141 tgtgtagtac cggtagccgt catggcattg tcttacatca ctgccatcaa cgtagggatc
     1201 aagacactag ctgtttgtat tatctgccaa attatcggtg catatttagg accaagattt
     1261 gtcgtgaagt taccagagaa aacgatcaaa ctgtttgtcg gcataggatt ggtcattgct
     1321 gcattgttga tttttgcagg acaaatgaac tggattccat caaacggaac agccagtgaa
     1381 ttgtatggcg gaaaattaat cttggcagga tttttactct ttgtctatgg tgcattgaac
     1441 aatatcggca ttggttctta tgcgttaacg atggtaactg tctacttgct aggcttgaat
     1501 ccagttgcag cctttcctat tatgatgggg gcttgtacct tctcagtacc gatcggcagt
     1561 gttcaattca ttaaatttaa tgaatacagc agaaaaatca cactttttac ttcaaccttc
     1621 ggtgtattgg gggtattggt cgctgtattc ctagtcaaat ctttagatac ctatatcctg
     1681 aaatggatcg tgattttggt cttactatac agtgcatata cgatgttggg cagtcaatta
     1741 aaaaaagtta cagcagctaa ttaaggagcg attcaaatgt tatttcttaa aaaagaagat
     1801 attatcaaaa gtttttcaat gagagaagcc atcgatgcgg acaaaaaagc actgtcattg
     1861 tattcagcag gaaaggcaag tgtgcctctt cgcaccaata tcgatgtgcc aaaaagcaac
     1921 ggacaaagct tatatatgcc tgcttatgta gaaggtggcg aaggcgcgct aggtgtcaaa
     1981 atcgtttccg tgtatcctga aaacatcaag aaaaacttac caagtgtacc agcaacgatg
     2041 atcgtattgg atccagaaac gggtatggta tccgcttgtt tggatggtac ttatttgaca
     2101 caattaagaa caggcgctgt tcaaggagca gcaacagaac ttcttgcaag agaagatgct
     2161 aaaattggcg cgctgattgg aaccggcgga caagcacagt cacaattaga agcaatgttg
     2221 accgttcgta aattagaaga agttcggatt tttgatattg attttgagcg tgccagccaa
     2281 tttgctgaaa aaatgatgca acaattttct gtaacgatgc gcccaacaaa aaccagtcaa
     2341 gaatgcgttg aaggagcaga catcattaca agtgtgacca cttcaaaacg tgcgacattc
     2401 tctgctgagt gggtcaaaaa aggtgcgcac atcaatggag tcggtgctta cacaccggaa
     2461 atgtgtgaaa ttccacgaga aataatcaaa gccgcagatg ttgttatttt tgataccatg
     2521 gacggtgttc taaaagaagc tggggacttc atttcaccgc tgcaagatgg gtatattcaa
     2581 agagacagct atcatgggga actaggccaa ctgatcaatg aagaattgat cggtcggaca
     2641 agtggagaac aaatcacgat cttcaaaaca gttggttcgg ctgtattaga tgtggtagtt
     2701 gcgacagaaa tcgttaagaa agctaaagaa aataatttgg gaaaattgat tttctaatta
     2761 aaaaggctga aattaaaacc tttattattg cgtttaaaaa aagaaaacaa agaagtttgt
     2821 aatcaaatgg aaactgaccc cataatttaa ataaaataaa aaacatctct tgttgaattc
     2881 tagtaatgaa gcgaccctaa aacttagacc aaaaaatcta acttttgggt ggagttgtca
     2941 agaaaaaccg ggcacttagt taaaaaataa gttggacggg ttatactaaa attgacacca
     3001 aattaagaga aagcgaagat ctggattatg ccaagacgtt catttgatga aatatttaaa
     3061 attaccgctg taaaactggt aacggaagaa gggttttctg ttaaagaagt tagtctatag
     3121 ctagaagctc gctctaatag cgtgtattga tgagttcaag aagttgaaaa atatggataa
     3181 cgtacttttc tcggtagagg aagtcatgaa atccattcgc agaaatgaaa ttacacgctt
     3241 agaaaaagag aataaacggt taaaagaata ggtagaactc ctaaaaatta ccgaagtctt
     3301 cctgaaagaa aaggctcttc gtcaaattgt gttggcgaag acaaatttga ataaaactta
     3361 gtccgatatg cggcgcgttc tgtgcgccgc
//
LOCUS       DAZBWR010000179         3384 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000179 DAZBWR010000000
VERSION     DAZBWR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.184"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..556
                     /locus_tag="ACHGT5_002762"
     CDS             <1..556
                     /locus_tag="ACHGT5_002762"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN7218691.1"
                     /translation="NKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIYG
                     EGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALA
                     IGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKG
                     IKLRKELTSKNISNIWYEYLNQFF"
     gene            575..1999
                     /gene="wzy"
                     /locus_tag="ACHGT5_002763"
     CDS             575..1999
                     /gene="wzy"
                     /locus_tag="ACHGT5_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFN7218692.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            2045..>3384
                     /locus_tag="ACHGT5_002764"
     CDS             2045..>3384
                     /locus_tag="ACHGT5_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN7218693.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGAT"
BASE COUNT         1125 a          434 c          616 g         1209 t
ORIGIN      
        1 taataaaact acaagtagta aaaaatttat tactgcatgt agattggata aacaaaaaaa
       61 tttgccctta atgattgatg caataggaaa acttattcat gaaggatatg agtgtgaact
      121 ggatatttat ggagaagggg gattaaaaac cgagcttgaa agttatatca accataatgg
      181 attaacagaa tttgttcatc taaaagaatt tacaaataaa atacatgaaa agatgcaaaa
      241 tgcggcagga tttgtaattt cttctgatta tgaaggtata tctaattcta tgttagaagc
      301 actggctatt ggagtgccag ttatcgctac agattgtcca gtaggtggcg caagaatgta
      361 tttaggtgat agaaatgaaa taggtcatct tgttgaatgt aataatctag ataagttaac
      421 agatgcaatg cgtagggtac ttttggatag agaaagtgtg gtaagaaaag cgcaaaaagg
      481 aattaaacta agaaaagaac tgacatctaa aaatatttca aatatatggt acgaatattt
      541 aaaccagttc ttttaaccaa aaggaggata ttgtgtgaaa gaaaaaataa atatattgat
      601 gttttcgtta acgttagtcg cgtttacaat attttgcgct ttttcattaa atcagaacta
      661 ttggctaaat tggcttggaa tattgattta cttatatgca atctgtacat ggcattggag
      721 aaaaggagat tcaatatttt cattatacac gatttttttc actctttttt tattatttag
      781 ttatgggcaa tgcattatgt gggcattcgg tattggaaca gatcgtggaa tcggtacaac
      841 agtagtagct tatggtacgg ggattattcc tagtgaatca gatatgataa tggtaaaatg
      901 gtattcttgt atttctatgt ttgtatttca cataggagca ctcttattta cgagaaaaac
      961 cacactaaga aaagttagtt ggtatgaatc tggagatgct gatgccgata gaaagttttt
     1021 atttaaaata gggtgtatca tttctataat agtagtaccg atagtttttg tttctaaatt
     1081 actagaggta cgtatagcat tagtccatgg ttataatgca ttatattatg gtgattatgc
     1141 tacacaaagt ggatatttac aaattattct atatcttttt tttccagcct taatatgttt
     1201 attaattggt agcaactttt caaaagtaat aacgagattt gtttttatta tttttgcttt
     1261 atattcttta ttaggaatta tgtcaggaga cagaggaagt tggttgtata gctctattat
     1321 actcatatgg gcatacactc aatataaagg aacctttaat tataaaaaat taataaagtg
     1381 gcttatttta gctgttatag gaatatatat tctaaacgta ataacgaaag cacgtgatgg
     1441 tggaggacta agtaaactta ctttaaaaga ttttacatct gtatttgatt cagatgattc
     1501 tcctttagtt gattcttttt ttgaaatggg ggggactatg agtattataa cttttttcct
     1561 aataagtggt aatggaatat atccctttgc taatacttat ttgacctcac tcttaggctc
     1621 tatatcaaca agaatgctat caatgtttgg aataaagttt gttttattag ctgattggtt
     1681 ttctcaggat tatttaggaa tcacttgggg gactggattc tcaatgattg cagaagccta
     1741 tgtcaatggt ggatacattg ggggtatagt ctatattttt attattggta tagtcatcgg
     1801 taaaatcctt tctaatagta tagacaacca agatattgaa aatcatccta tgaagatgtt
     1861 tttggctata actacagcaa atattataat gggttttcct agagctgctt catatttaat
     1921 tgtgaaaaat tttgtgtatg gtgttttaat tatattgttg ataaactttt tcctacagca
     1981 atttaataaa cgtctttaat atttaagctt aataaaaaga taaaacaagt caggagttgc
     2041 aaaaatggaa agaactgctc ggctcaaaaa aaacactatt tgggctctgg tgtttcaaat
     2101 aacttctatt gtctgtggta tgatcttgct acgtttgata ttaacaaagt acggatccga
     2161 ggttaatgga atgattagct ctattaacca atttctacaa attatttctt ttttacaatt
     2221 aggagtaggc gctgtaatgc aatcaactct atacaaacca ctagcagaaa aggataattt
     2281 aatgataagt caaatcttag cttctggagg gaagttttat cggcaaattg catatattct
     2341 actagtatac gtgggtttgt tacttgcagt ctattatgtt ctcgttccta atgaatttgg
     2401 gagatcatat acaatggcaa tgattgcaac attaagtatt aattcatttg ctcaatatta
     2461 ttttggtatt gttaatactt tgttattaag tgctgaccag aaaatttata ttacgtatat
     2521 tgcacagaca atagcattta tttctaatac agttgcatgt gctttattaa ttagtctgaa
     2581 cacagagatc cttatggtaa aacttattac tgctataatt tatcttgctt gtccaatata
     2641 ttatagatgg tatgtggatc gaaactataa tataaagagg aatgtgtcat atgataagga
     2701 accgatccct caaaaatgga atggggttgc acaacacgta gcttctgttg tactagatag
     2761 tactgatgtt gtagtgttat ctatattttc taacctgata tatgtatcta tatataatgt
     2821 gtacaattta gtaatatacg gagtaaaaca gctattcatg gctatgacaa atggcgttca
     2881 ggccttacta ggggaacttt gggtcagaaa agaaatagaa gttctttaca aaacatttgc
     2941 atggttcgaa tggattgtac atactttgac agtttttatt tttggctgta ccggtatact
     3001 aatcgttcca tttattcaag tatatacaag aggagtaaca gatgctaatt ataatcaatt
     3061 atcattcgca gctttacttg taactgctaa cgcacttcac tgtctgcgta caccttatca
     3121 tgtaatgata aaagcagctg gtaaatataa agaaactcaa aatagtttta taattgcttc
     3181 tttgttaaat ttaggtttat cattaatttt ggttaaacca ctagggctag ttggagtagc
     3241 attaggatct ctaatttcaa tcacatatca tacactttgg agtgcaaatt ataattcaaa
     3301 atatttgctt aaatggccga tgaaaaaatt ctataaacag ttatttgtaa acatcttaac
     3361 tgtagttctt tcttttgggg cgac
//
LOCUS       DAZBWR010000180         3356 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000180 DAZBWR010000000
VERSION     DAZBWR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.185"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACHGT5_002765"
     CDS             <1..189
                     /locus_tag="ACHGT5_002765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218694.1"
                     /translation="DLEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIE
                     DWAIFMAKEGKEMFQNKG"
     gene            202..477
                     /locus_tag="ACHGT5_002766"
                     /pseudo
     CDS             202..477
                     /locus_tag="ACHGT5_002766"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            504..803
                     /locus_tag="ACHGT5_002767"
     CDS             504..803
                     /locus_tag="ACHGT5_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFN7218695.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            977..1528
                     /locus_tag="ACHGT5_002768"
     CDS             977..1528
                     /locus_tag="ACHGT5_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFN7218696.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1674..1967
                     /locus_tag="ACHGT5_002769"
     CDS             1674..1967
                     /locus_tag="ACHGT5_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFN7218697.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1960..2256
                     /locus_tag="ACHGT5_002770"
     CDS             1960..2256
                     /locus_tag="ACHGT5_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218698.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2331..3329
                     /locus_tag="ACHGT5_002771"
     CDS             2331..3329
                     /locus_tag="ACHGT5_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN7218699.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1264 a          459 c          664 g          969 t
ORIGIN      
        1 gatttagaaa aacttactaa acaaagagat atgaaaaact ttaataattg tgtagttttg
       61 ataattataa ctgaattaac tttatttaca aatatatatt atatccatga agcccaatct
      121 tctttaatag aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat
      181 aaaggataac gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct
      241 ttatgttgga atctaatcga tcaaaacgtt caaaagcaat ttcctttaga ctatcttcaa
      301 atttttgaat tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaaccg
      361 gaagaaagaa aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa
      421 atatgggcca ttgattcggg tgagtatcaa acgatgttat taccagaaga atactagtat
      481 ctaaaagaaa ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg
      541 cacagctggt cactgcttct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg
      601 aaattgaaat gggtatgctt cctgaagatg ttcatctacc acgtttagtg atgacacatc
      661 tttatattta ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata
      721 ttaccagcca gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc
      781 agattggagg aacggatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa
      841 gatatcctta ccgcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt
      901 ttcaactagt attgacactt ataacggcaa tggtaattga agtgcttgaa caaaaaatag
      961 cataggagaa aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg
     1021 agatcctcat catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga
     1081 agcggaagat aaattagcag tagtcattac tattcgtcca acagatacag aaccatttac
     1141 gaatgatttg agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt
     1201 tattgctgtg aatctttttt cgtacacaaa agcgaaaagt cctcgggtat ttcttcgagg
     1261 aaatgatgaa caatctttgg aaatattaac gactgttttt acagaaaaga aaatcaatac
     1321 cattattttt gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcaaa
     1381 gactatttac gatcaattga gtgcgaaaca aaagaagatg accaaagtat tgataaatgc
     1441 agaaggtaag ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga
     1501 tagtcaatta ttatttcaag aaaaataaca ttttattttc tcatgttact tataagtagt
     1561 tttacatgct ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat
     1621 gtacccagta atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga
     1681 ttgatgaaac ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg
     1741 aactggattc agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt
     1801 ttccaatgaa aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac
     1861 aggaaaaaac tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga
     1921 ttcaaaaagt aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta
     1981 ggtggtttaa tttacggtgg atattggtta tatcaatata tgcttgcgca tccgaatata
     2041 gataaaggaa aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact
     2101 aagaaaaata atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga
     2161 agggaaattt ctactagttt taatccatct gagagtcctc aaggaagaga ccgctgccat
     2221 acgggctatc gacattattg ggaaagaatc gattaaaaga ttaccattta gatcaattaa
     2281 ttgtctgata gaataagtac gtgagggaga aattgaaagg gggaaatata atgaaaaaat
     2341 ggctgttatt actaatagga atgggaattt tattcactgg ttgtacatct gagaatacga
     2401 ggactaaagc gactatagaa actgatacaa caattgagac taaaaaaagt aaaacgcaat
     2461 caactgaaca aactacaaca gaaatgaagc aatctgtgga aacaagtgca agttcaatca
     2521 ctgaatcttc ttctgaagag gttaagaata ctttgtggga tacgaataaa gcttccaaat
     2581 tagaaacttt tgtcacacaa tggggaaaga cattaggtca agaatataaa agctacaatc
     2641 ttcagaataa tgtaagtctg tatggcactc ctttaccaca agccgtgatt aatggcgatt
     2701 ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg gtcagaagat ggaactggtc
     2761 atgcagattt caatttagta gcagtttatt ctgatgtaga aactggagaa tatttagggc
     2821 agcatgttta ctttttcggt tttcaaaatg gacaacctaa agtttattta acgcaacaaa
     2881 atcaaggtaa tgaaaataat tatctgtact ttaacgaaac tcaaaatcaa cagttaaaac
     2941 aaggtttcag tgatattgtg aatggacaaa ctcctcaaac accagtagta gaagaatcta
     3001 ctcaacaagc aacaactaca acgacacaat ctacaagtag ttcaggaatt tcaagttggg
     3061 aagctgctaa agaatctgtg ctaaataata aagctttatg gttgtatgat tctcctgata
     3121 aaaatgtatc aaatactaat ttaattagca caaatgaata tatgaaagat ctaatgcaag
     3181 atgaaaatgg aagctactat tcaattaata ttgtaacgga tgatgatgga aacacgacca
     3241 tggctggtgg aaaacaattt cgtgtttata cagatggaag aatttctcaa cgtattggaa
     3301 tgactaacaa ttttcaaatg attaaataat tgaaaatatt atttatcaaa gaaaaa
//
LOCUS       DAZBWR010000181         3301 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000181 DAZBWR010000000
VERSION     DAZBWR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.186"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(475..750)
                     /locus_tag="ACHGT5_002772"
     CDS             complement(475..750)
                     /locus_tag="ACHGT5_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN7218700.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            857..1621
                     /locus_tag="ACHGT5_002773"
     CDS             857..1621
                     /locus_tag="ACHGT5_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN7218701.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1744..2247
                     /locus_tag="ACHGT5_002774"
     CDS             1744..2247
                     /locus_tag="ACHGT5_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN7218702.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2526..3173
                     /locus_tag="ACHGT5_002775"
     CDS             2526..3173
                     /locus_tag="ACHGT5_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN7218703.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     regulatory      3215..3281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1100 a          587 c          675 g          939 t
ORIGIN      
        1 aaaaactttt ttgttctgtc tggaaaatat gggttaagca aattcattgc aagttataga
       61 tcagcttttt tactacctgt ctttaatact tgaggcaaag cgcctcaaaa acaaccttat
      121 cgacggtgtt caaaagcttg catctacggc attaagtcca gcctgttgaa aaatatggag
      181 agctatttat cacaaccagt ccttaatgct tgatgcagac cagctttttc acaaccttat
      241 tgacggtgtt ttatcagctg cctctcggta ttaagtcatt acatttcaaa atatgagaag
      301 ctattttgaa aatgtttctc cttaatactc gaggcaaagc gcttcaaaaa caaccttatc
      361 gacggtgttc aaaagtcagt ttctcggtat taagtcaaat ttatgcaaaa atgtggaaag
      421 ccattttcac aaaattttcc ttaatactca aaactaagcg accttttcac aaccttatcc
      481 cactacatca aatttctttc gttcctcaat catcggatct tcttggacat ccacatactg
      541 cactcgtcca cttgggctcg gagacttctt gacttcttcg ataaattttt ggatgatgtc
      601 gtcatttccg attgcttcga ttgtgactga gccgtcgtct tcgtttttta ccgttccggt
      661 cacgcctaac tgatcagcaa ccatcttggt catatagcga aatcccacgc cctggacacg
      721 tccttggaca ttcattttaa ctttgcgcat gttcgtcacc tttctttcct gttttataaa
      781 tcaatgatac cgattttttt aggatgaaac aagctcaagc acctattcca tgatataatc
      841 agttgaggtg aggaatgtga aagagatcca atcagcaaaa aatccaagaa tcaaagaatt
      901 aaaaaaacta cataaaaaga aatacagaga agaaaaggca gaatatcttt tggaagggtt
      961 ccatctgatc gaagaagctg cgtcgtctaa tgcagaaata aaagaacttt ttgtgacaca
     1021 gagaggtatg aaagaatggg gagactggct ggctgtaaac aaagagattt ctacttttct
     1081 agtttcagat gaggtcatga ccgctatgtc agatctacct acaccgcaag ggatcttagc
     1141 agtgatgacg ttaacatctg aagatacgcc agatttttca ggaggctggc ttttactaga
     1201 taatgtacag gatcctggaa atgtgggaac gatgatccgt acggcagatg cgtttggtat
     1261 ggctggtgtt ttattaggta aaggaacggc agatatttat agtacaaagg tgctgcgaag
     1321 catgcaggga agcaattatc atttgcctgt gttacaaaaa gatttggcag atgtaatccc
     1381 tgtaatgaaa ggcaaagggt ttacggtgta cggtacagaa ttaaatgaag aagcacatgc
     1441 attagacaaa gtagaaaaaa cagaggattt cgctataatc atgggaaacg aaggtcaggg
     1501 agtaagtcag gaaatcttgt ctttgactga tcagaacgta tatatcccaa tgaaaggaaa
     1561 tgcggaatca ttgaatgtcg gagtcgcagc agggatcgtt ctttatcatt tttctttatg
     1621 attaaggaac tattaagctt tagctatttt atctagagcg actagaataa tccgaacact
     1681 ggtggtatac ttagatcaat ttcagaagaa aatgaaattc aaaaaggtgg cagatcgata
     1741 attatgacag aaaaatggcg tgaagacgaa gaatatatgt cttatgtaca agatctttta
     1801 gaaacagaag cagtgaaaaa attagcgaat tacacacagc atgttcattc cactcgtttg
     1861 gagcattcca tcagtgtttc ttactatagt tatctgctcg caaaaaaatg gggtggcaat
     1921 gctaaagcaa cagcgcgtgc tggacttttg catgatctgt tctactatga ttggcgtacg
     1981 acgaaatttg acgaggggac acatgcgtat atccatccaa gaattgctgt aaagaatgca
     2041 gaaaaaatca ccgatttgtc ggatcttgag cgagatatca ttttgaagca catgtggggt
     2101 gcaacgatcg caccgcctaa gtataaagaa ggatatattg tgacattcgt ggacaaatac
     2161 tgcgcagtca aagaagcggc tcagcccatg tctgcaagta tgcgtaagag atggcaacgt
     2221 tactttggga aagaatcctc gatttaatag gtaaaaccaa taataataga ctatattccg
     2281 agaaaatctg gctgattttc tcggtttttt ctgtttgcag ttgactatct gttcaagata
     2341 cggtttactt ttatttatcg aatgaatcat gtacatagaa gaatgaacat taaagggggt
     2401 gatgataaga tgcaaaacgt atcttattta aaagagtccg ggatcttcag gtaacacata
     2461 ctttgcagaa gatcactttt ttctacaaag tatgcacttt ggttagaaga aaggacttta
     2521 aaatcatgga acaaacaata aaaccatacc aatatttttt tataaaaaga gaagtagatc
     2581 agttattaaa tgcgtactca tctgtcaatg atccaaaaac ggtacaaaca gttcaggcac
     2641 tagcagctga aaagattcga gacatattag aacacgaact gccagaaatc gatacatttt
     2701 taacgacagt tttagacgta aaactgacaa aagcgcaagc agaaagagca ttggaggagc
     2761 tgaagcaaat cgtccagccg tttatgcagc ctagcaaacc acaaatcgag aaattattcc
     2821 gaaaagtgaa aaaactcaaa caaccagctt ggaatgaaat cgacttgcaa gaacatacgt
     2881 acatcggctg gaatgatcca ggtacgcaga aaaaattcat ggtctattac gaagatgaaa
     2941 aattaaaagg gattagcggt actttatcac caacgataaa taagggaatc tgtgcaatct
     3001 gtcagaaaac atcgaatgtg tcgctttttc tttctacaac gaaagcaggt tctgatggca
     3061 cgtatacgaa aaaggggaat tatatttgtc atgatagtga ccaatgtaat caacagttaa
     3121 cgaaattggc tcctttctat gaatttgctg aacgtgtaaa gaaagaacga taattttatt
     3181 gttttttaca gaaaaatatg ctatctttta atggaagtga ataggtgatg gtgttcgcct
     3241 ttaaatgcta acgaaagata gctaatgacg cctactaaag tctggggata gcccctttct
     3301 t
//
LOCUS       DAZBWR010000182         3247 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000182 DAZBWR010000000
VERSION     DAZBWR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.187"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..538
                     /locus_tag="ACHGT5_002776"
     CDS             <1..538
                     /locus_tag="ACHGT5_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN7218704.1"
                     /translation="LQVFHPVVTELENIEKQILALINSMEQQSQPIICILPNSDAGSE
                     IIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGT
                     RQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLN
                     QILNDGEDIKWIQKQIAY"
     gene            511..1188
                     /locus_tag="ACHGT5_002777"
     CDS             511..1188
                     /locus_tag="ACHGT5_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN7218705.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1139..2320
                     /locus_tag="ACHGT5_002778"
     CDS             1139..2320
                     /locus_tag="ACHGT5_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN7218706.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2333..>3247
                     /locus_tag="ACHGT5_002779"
     CDS             2333..>3247
                     /locus_tag="ACHGT5_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFN7218707.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEP"
BASE COUNT         1208 a          417 c          620 g         1002 t
ORIGIN      
        1 tttgcaagta tttcatccag ttgtaaccga actagaaaat atagaaaaac aaattttagc
       61 tttgatcaat tcaatggaac aacaatctca accaattatt tgcattttac ctaatagtga
      121 tgctggtagc gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg atattattta
      181 tattacgaat ttagagccgg aggaatacct gactatatta aaagagtgca aatttatgat
      241 cggaaattct tcttctggga taattgaagc tgcatcattc aaaataccag taataaattt
      301 aggaaccaga caaaatggac gagaacaatc taaaaatatt ataaatgcaa atctaaatga
      361 aaatgaaatt gaaactgctt tgaataaaat acaagatcct cagtttataa aaagcttgaa
      421 aaatgttaag aacatctacg gtgacggcaa cagttctaga agaataacaa aaacattgaa
      481 tcaaatattg aatgatgggg aagatattaa gtggattcaa aaacaaatag cttattaaaa
      541 tatactagtg atccatacat tattattggg gctggcggac acgcaaaagt cattattgaa
      601 accttaagat tagaaaataa gttaatatat ggattttttg atgataagca aaataaaatg
      661 caagatctga aatatcttgg gaaaataaac gaattgaata gctgggcgga tagcaaaaaa
      721 aaaatctata aatttattgt agctattggt gactgtgtag taagaaaaaa gattataaaa
      781 aaattgaata tgccaagtga gcaatatgga atagctgttc atccaagtgc tatagtggca
      841 aaagaaattt caattggtta tggaactgtt atttttgcga atgctgtaat taacccagat
      901 gctgtaattg gagagcatgc tataataaac acaggtagca ttgttgagca tgattgtaga
      961 attaataact atgttcattt atctccaggt gtatgcttag cgggtggagt acatgtaggt
     1021 gtaggaactc aaataggaat tggttctcaa tgtatccaga tgaagaaaat tggatcttgg
     1081 tgtatgatag gcgcagggag cactattgtt aaggatattc cctcacattc cttagcttat
     1141 ggcaatccag ctaaaattaa gaaagaagga attaattttg aattctagaa ttttattatc
     1201 aagtcctcat atgggaggaa cagaagaaaa atatgttgct aaggctttta aagataattg
     1261 gatagcgcca ttaggtgaaa atgtggataa atttgaagaa agtattacaa aatatactgg
     1321 ttctaagggc ggagcttctg cgttaagttc aggtacagct gccatacatc ttgccttaag
     1381 actgcttaat gttggaaaaa acgatattgt ctttgctcct tcatttactt ttatagctac
     1441 agtaaatcct attttatatc aaggggctgt accagtgttc attgactcag aacctaatac
     1501 atggaatatg agccctgttg cactagagag cgcattagaa gaagctgaaa gaaacaacca
     1561 attacctaag gcaatcatcg tagtgaattt atatggtcaa atggctgatt atgaacgaat
     1621 tttagctatt gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg
     1681 ttctacttat aagggaattt cgtcaggctt atttgggaca attgggatct attcatttaa
     1741 cggtaataag atcatcacaa cctcaggtgg aggagctcta gtgtctagtg ataaaaaata
     1801 tgttgagcat tcaagatttt tagcaacaca agcaagagat caagcaccgt attatcaaca
     1861 tagcgaaata ggttataatt accgtctaag caatatttct gccggtatcg gacgtggaca
     1921 aatggaagta ttgaatgata gagtagctaa acgaagagaa attttcaatc gatataagga
     1981 agctttttct ggaattgata gaattgaaat gatgccagaa gtggatggca tggtgccaaa
     2041 tcgttggttg tctactttaa cgatcaatga ttataattct attgatcaag tagttaacgt
     2101 aataaataaa ctaaatgaaa aaaacattga aacaagagct ttgtggaaac cactacattt
     2161 gcaaccagta tttcaagggg taaaaatgta ttaccatgat aataatctta gtgtgtcaga
     2221 aaagttattt aatagcggct tatgcttacc ttctggaagt aatatgacag atagtgaaca
     2281 agaatttgtt attgatgagt ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat
     2341 aggaattatt ggtttaggtt ccatggggaa aagaagactt agactcattc aagaacactt
     2401 taatgatatt ttactatgtg gtgttgatac taatgaggcg agacgaaagg aagttgaaaa
     2461 ggaatttgga ataccaactt ataatagtct aacagaggca aaaaatttgt ttgaaataaa
     2521 ttcagttttt gtttgtacat cccctataac ccatgaattt attataaaag aggctttaaa
     2581 tttagattgt aatatcttta cggaaatcaa tttattaaat gaatattatg atgaaataat
     2641 cgaagtagct aaaaagaaag atagattact ttatctatct tctacatttt tgaagagaaa
     2701 agaaattcaa tatattaagt ctaaagtgac gcataataaa aaaatttctt atcaatacca
     2761 tgtaggacag tatttacccg actggcatcc ttgggaagat tatcgcaatt tttttgtttc
     2821 aaacaaaaaa actaatgggt gtagagaaat attcgctatt gaattacctt ggattataga
     2881 tacatttggg aaagttaaat cgtttactgt aatgacccag aaattatcca atcttgatat
     2941 tgattattcg gacagtttca ctcttattat tgagcatgaa actggtatag ttggaacttt
     3001 aagtgtagat gttgtttcaa gagtagctaa aagaaattta actataattg gtgaaaagat
     3061 tttaatagaa tgggaaggta acccggattc tctattttca tggaatgaag aaacttctag
     3121 tatgacagat attcaattgt acgatgaggt tatgcatgat aataattatt cacatactat
     3181 tattgaagat gcatatttag aggagattaa agaatttttc acgcttttgg aaaagaaaat
     3241 ggaaccc
//
LOCUS       DAZBWR010000183         3206 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000183 DAZBWR010000000
VERSION     DAZBWR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.188"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(423..1037)
                     /locus_tag="ACHGT5_002780"
     CDS             complement(423..1037)
                     /locus_tag="ACHGT5_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN7218708.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1643..2020)
                     /locus_tag="ACHGT5_002781"
     CDS             complement(1643..2020)
                     /locus_tag="ACHGT5_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218709.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2320..3201)
                     /gene="galU"
                     /locus_tag="ACHGT5_002782"
     CDS             complement(2320..3201)
                     /gene="galU"
                     /locus_tag="ACHGT5_002782"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN7218710.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          976 a          660 c          497 g         1073 t
ORIGIN      
        1 ctattagaaa aaggtatcct gttggacaat tttgttatat caggtaaact tcattgcaat
       61 aatgataaca aatgtactat aattactttg taaagagttt tggggaagac tctttcatca
      121 acgtgtatta agagacaaga aagaatccat atggattctt tcttgtctct ttttccacta
      181 ctttttattt ttctgacact acatattctt ataactcatt tctcataact gtgaaacaac
      241 ttataaaaat aacaaacttt aaacaaaaca agcttaaaat caaagatgat ccatcttaaa
      301 ataactaaaa caaaaaaagt agtcgttgtt aatcctcatt aataatcgtt ttcttttttt
      361 caaacgctcc atttttatgt ggaacattat ttttttaagg aactttgtca acatgtggat
      421 aactattttc cacttgtgtc ctggactagc tgttttgctg taaaatacaa aacttgctgc
      481 agcaaaaaga tcgttgcaag agaggctgtt ccgcagatcg ctggatctag atttggcaaa
      541 gtcaagactg catcatgtaa attggcgtgg ttgtccaaaa ccaaatcagc aaattcaaat
      601 aatcgcaaac cactgctatg ttgcgatttg atacttcgtg acaaatcaaa tccagtaaca
      661 atgattaatg gatgaccatt ttctttgatc cactgagcta gttcgatgat tgccgggttt
      721 cttccttcat tcgaaagaag aagaaaaatg tcttctgatt tgacagaaag ctgatcaata
      781 attgcctgtc ctgcaccttc tagcgtttca tagacacctc cggctggatt ttcaatcaat
      841 atacttggta caagatccgg ggtttgatcc caaaattcaa atgctacacc acttagtgtg
      901 cggctagcat atatctgcaa tctaccacct tgattaattt gtttcgcgat ccaagtgcca
      961 gcttgaagca tcagcggctc ttctatcctt aatttctctt ccaattggtg ataatagtca
     1021 aaataacata aactcattta tctctcccgc ctagctatac ttgacatgaa tagaaacagc
     1081 tgcctaatct aaggaagtac ataaaaaaac agtagttaga cagtaacaca tgtggtcttt
     1141 gctgtttcaa acacaaaaaa ggcattgaac gaatacgccc aataccttga catagcaaat
     1201 aaaacaacgt gcagaccatg gaaaatatct acataggggt agaattttca ccctagatta
     1261 ggtacaataa attaggtcga tgcactgcat tgactatggc aaaatgatcg gtcagggtgt
     1321 atgtgtgtga ctggtcattc cctgattctt agcgccaact aagggtcagg gagccttttt
     1381 ttattcagct gtttctatct gaatttattg taagtaatct cttatccaag tgcaagcaag
     1441 aaaatacaaa aaaacattgg tatgatttat atatatataa ataacataaa aaaaggaatg
     1501 gcttatcccg atacaacaag cattccttta aaaaagattc ttttatataa gtgaaaaaag
     1561 cttttttcca ccaattaaat aaatctctca tctcaatagt ttatccatta ttttatcttt
     1621 ttttgcgtat acctactaaa tattaccgtc ttcttctcgt tcttctactt tgttcgagag
     1681 aatctgtact agaaaaagta gttgtatgtc taccttttct gcctatccga gactttcttt
     1741 tcatatatgg gatataaatg attagataca gtacagcaaa aaataaagca aatacgcaag
     1801 cttcccaact acctaacgcc aattcaacca tcaaggtagc aaccccaacg gatgctgcat
     1861 accagataaa aatcagaaac gtttcttttt ttctgaccat gcgaaacatg ataaatagac
     1921 taataagcaa tagtggcaac acaaatatcc aattgcttcc ttccatcagc cacttgagta
     1981 tatcaagcag cgactgtgca acagcgacta attttcccat agtgaacaca cacctttttt
     2041 tataattaaa aaattcgcct ttttattcta ctatgaatct tctggcttgg ctttcatttc
     2101 tcttatgtaa tcattttctt ttatacaact taaaaaaaaa ttaaaccatt agcataatat
     2161 tttctgaatc tattcacgaa agtctttttc tatctttcgt tttccacgac tcaactgatc
     2221 tagagtcaaa aaactaaaac gacagttgcg caaatgacgc acaagctgcc gctttaataa
     2281 caaatagcct ttcattctca gcaggccact tatttccagt taactttctg ttcttagctt
     2341 tttactcaac tggataatat atgcacttaa tgaagtccca atttccgaat gttgaagtcc
     2401 atattcaata tttgtcttca tatatcctaa cttatcgcct acgtcgtatc tttttccttt
     2461 aaactctctc gcatagaccg cctctgtctg gttaagagtc tgaagtgcat ccgttagctg
     2521 tatctcgttt cctgctcctg gttcttgagt ttctaatatc tcaaatattt taggagtcag
     2581 tagataacgt ccgataatgg ctaaattact tggtgctttt cccatttcag gtttttctac
     2641 aaaccctttt actctgcaac gacccttttc gctatctgtt ttaggatcaa taattccgta
     2701 tttagatgtt tcattttctg gtacacgcat gacggcaaca gcggaagctt gtgtttcttc
     2761 ataatccatc atcaattgtt gagtcaatgg gatatcatct ttcatcaaat catcccccag
     2821 catgacgaca aacggttcat ttcctataaa tgcttttgct tgaagcacgg catcccccag
     2881 tcctcttgga tgagattgac ggatgaagtg aagattgact tgagtcgttt cttcaactaa
     2941 agcgagtaat tctgtttttt ccttttcccg caaattgttt tccaattcga tatttgcatc
     3001 aaagtgatct tcaatgggtc ttttttcttt tcccgttacg atcaagatgt cttcgattcc
     3061 cgaatccaat gcttcttcca caataaactg gatcgttggt ttatccacga ttggaagcat
     3121 ttcttttgcc atggcttttg ttgccggcaa aaaacgtgtt cctagcccag cggcaggaat
     3181 gactgctttt ttgaccttca cttgct
//
LOCUS       DAZBWR010000184         3199 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000184 DAZBWR010000000
VERSION     DAZBWR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.189"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHGT5_002783"
     CDS             301..585
                     /locus_tag="ACHGT5_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218711.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHGT5_002784"
     CDS             588..1004
                     /locus_tag="ACHGT5_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218712.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHGT5_002785"
     CDS             complement(1141..1860)
                     /locus_tag="ACHGT5_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN7218713.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACHGT5_002786"
     CDS             2522..2608
                     /locus_tag="ACHGT5_002786"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN7218714.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACHGT5_002787"
     CDS             complement(2910..>3199)
                     /locus_tag="ACHGT5_002787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218715.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAZBWR010000185         3120 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000185 DAZBWR010000000
VERSION     DAZBWR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.190"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..747
                     /locus_tag="ACHGT5_002788"
     CDS             58..747
                     /locus_tag="ACHGT5_002788"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN7218716.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            761..2263
                     /locus_tag="ACHGT5_002789"
     CDS             761..2263
                     /locus_tag="ACHGT5_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN7218717.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2276..2752
                     /locus_tag="ACHGT5_002790"
     CDS             2276..2752
                     /locus_tag="ACHGT5_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN7218718.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2849..2959)
                     /locus_tag="ACHGT5_002791"
                     /pseudo
     CDS             complement(2849..2959)
                     /locus_tag="ACHGT5_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1029 a          415 c          612 g         1064 t
ORIGIN      
        1 tatattgttt ttggagttat tatccaaaaa taataaaaaa ttggagcgtt taggataatg
       61 gataaaaaga ggtttttaga tcttatttca aaaggtttga ttgtttcatg tcaagcatta
      121 cctggtgaac cattttacaa agaggaagaa agtttaatgc cttattttgc tttggctgct
      181 caaagggctg gagcagttgg tatacgtgca aattctgttc gagatattat tgaaattaga
      241 aagtgtgtat cattaccaat gattggaatt attaaaagag attatccacc agaatctcca
      301 ttcattactg ccactatgag agaagtagat gaattagtag aaaccggtgt tgaggtaatc
      361 gctcttgatt gcactcttag aaatcgacat gatggactat ctataaattc ttttattact
      421 aaaataaaag aaaaatatcc tcatcagtta tttatggcag atatttctac atttgaagaa
      481 ggcaaaaatg catataaagc aggaatagat tttgtaggaa ctactttaag tggatatact
      541 gaggaaagta aaaaacaaga tgggccagat atagatttga ttaaaaaatt ggtagaagaa
      601 gggatttgtg taattgctga gggaaggatt catactccag aacaagcaaa agagataaaa
      661 aaaataggag ttgcaggtat tgttgtgggt ggtgctatta ctcgaccaca ggaaattgca
      721 tcaagatttg ttaaagcatt aagttagggg gagaaataaa atgtttaaaa aattttcaca
      781 attaggacgc gctttcatgt taccaattgc tatattacca gtagccggat tactattggg
      841 actgggagga gcgttaacaa atgagagtgc tataaatgct tatccatttt tagatcaacc
      901 gtggttgcat acaattttaa gtattatgag ttatgctgga aatgcagttt ttgcaaacct
      961 agcactaatt tttgcaattg gtattgctgt aggacttgca aatggagata aaggaacagc
     1021 tggtttagct ggtggagtag cttatctagt atatactgca actattagtg ggtttcttga
     1081 acttttttca gcaaaagatg caacactaga tacaggtgta gtgggatcaa ttgttgttgg
     1141 tggtactgtt gcatttttac acaatcgtta tagaaaaatt gaattaccac aattcttagg
     1201 tttttttgga ggatcaagat ttattccgat tgtttctagt ttagctgcaa tcattattgg
     1261 ttctttcttt tatttaattt ggccaccaat tcaaaatggt ttagtagttg ctggtgaaca
     1321 aattgcccaa atgggtagtt taggaacttt tttatatggt tttttattgc gtttaacagg
     1381 tgctgttgga ctacaccaca ccatttaccc tttgttctgg tatacatctt taggtggtac
     1441 cgaaactgta gctggatcaa caattgctgg agcccaaaat attttctttg cccaactagc
     1501 tgatccaaac cacactggtc tgtttactta tggtactaga ttttttgcag gaagatttgc
     1561 aacaatgatg tttggattac cagcagcatg ttatgcaatg tatcgtgcaa ttcctaagaa
     1621 aaatcgtaaa aagaatggtg ggctttattt tagcggggcc cttacatcat ttttaactgg
     1681 aattactgaa ccagttgaat atatgttttt atttgttgct ccttggcttt atgtgattca
     1741 tgcattctta gatggattat ctttttattt tgctgatatc ttaaatattc gtgtagggaa
     1801 ttccttttca ggtggtttga tcgacttttt attattcggt gtacttcaag ggaatgacaa
     1861 aactaattgg ttaaaattaa ttccttttgg tattgcatgg gcatttatat atttctttgt
     1921 ttttacattc tttatcaaaa aatttaaggt agctattcct ggaatggaag aagaatcaga
     1981 agttcattct gaaaaagtaa taaacaaaaa tacatcatta catgaagagt ctcttatcat
     2041 tattgaagct cttggtggaa aagaaaatat tgagtctgtt gaagcttgtg caactcgttt
     2101 gcgtgttgct gttacgaatg gggagattat tcaaaaacaa acaataaaag acactggtgc
     2161 aactgctgtc ttcgaagtga agggtggcat ccaagctgtt tttggaggga aagctgactt
     2221 attaagtcaa gaaatcaatc aaatattagg aaaagataat taggaggttt ttaatatgtt
     2281 tgaaattttc aaaaagaaaa aacaagaaac tttttatgca ccttgttccg gaaaaatcat
     2341 ttccatcaca gaagtaaatg atcctgtctt ttcacaaaaa ttactaggtg atggttttgc
     2401 cgtcatacct gatggttcaa aaatttatgc tccattggct ggtcgagtaa caaatatatt
     2461 tcctactaaa catgctatta atataaaaag caactcaggc attgattatt tgattcatat
     2521 tggaattgat acagttgaat tacaaggaga gcctttttcg atttgtgttc aagaaaatga
     2581 tgaggttact cctgaaacct cgttggttga tgtggatttt gacaaaataa taaaatctgg
     2641 taaagatact tctgttatag ttgtttttcc ggagaaaaat cagttcaatg atttaataat
     2701 agataatggt cgaattgctc atgggaattc atgtggaaaa atcacttttt aaaaaagtgt
     2761 atataaaaag ctataaaata agctaaatag gatatttgtc aaatgaagtt ggtgaacaga
     2821 aagaactgtt ttctttctgt tcattttttt attttattag taatgattcc ccatgccaaa
     2881 tcatgatccg taatttaaag ttataaaagc ttcgaaatcc attggcattt cgttggaata
     2941 ccttgatatg attattcaaa tataaaatat agagagaaga gagagaggga agatatttat
     3001 aaagggcaaa gttatgttta ctcaacttag gaaatagaaa tagatcaatt tatattctct
     3061 tgtcaatcga atatgaacct tagcactata taccaaatat agtaaaatgc tgaaagatgt
//
LOCUS       DAZBWR010000186         2959 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000186 DAZBWR010000000
VERSION     DAZBWR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.191"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            718..1560
                     /locus_tag="ACHGT5_002792"
                     /pseudo
     CDS             718..1560
                     /locus_tag="ACHGT5_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            1682..>2959
                     /locus_tag="ACHGT5_002793"
     CDS             1682..>2959
                     /locus_tag="ACHGT5_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN7218719.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGK"
BASE COUNT          918 a          542 c          579 g          920 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agacatcatc cataaaatgg actttgtcta cagtctggat aacgtgattt tttgctgtaa
      181 tgacaaggtt ttagctgttt cagataccat gtatccaaaa atagtttatt gagaaaatag
      241 gagagtattt gatactaact agtaggaaaa aatgagataa aaagtaaatg gaatcattta
      301 aggtaatctg aaataaaaaa tatacagccc tgtggtactt tacctacaag gctgtatata
      361 ttttttactc tcagcgtttt tgactccgtg tctgactccg ttttattgat tttgaatgtt
      421 attcaatgac catttaacag gtcaaatttg atgtttttga ttccgtatga cgccgtttga
      481 tatctagtga tatagtgatt tgagtctaca tcgaataagg gaataaacca ataaaaaatg
      541 atttagaata ttaaaataac aaaaaagagg actacctgta ttagtcctct tttttgacca
      601 gattttgacc agtctatttt acatcctagt atatttatat gagctaattt tatgattaaa
      661 tggtaagttt ttccttgtgt gttttagttt aaactagtgt gtaattcaaa aatctatatg
      721 gaaggaacag tattaagaat agaacagggt tctctacatg atggtgctgg tttacggacc
      781 gttgtttatt taaagggctg cccattacgc tgtgcctggt gttcgattcc cgaatcacaa
      841 agcaagcaga ttgaaaaagg atttggccag accatgaccg ctgaagaggt aatggatgag
      901 attgagaagg atgctgtttt ttactttcac tcggatggcg gagtgaccat tagcggaggc
      961 gaggcactgg ttcaggcgga ttttgctaaa gaaattttgc aaaaatcgaa atacattgga
     1021 atcaataccg tactggaaac cagcttttgt ggtgcttata atgaaatcca aaaggtagct
     1081 ccctatgtgg atacattgtt tgtggatgta aaaatgttta ccagcaagct ccacaaacaa
     1141 tggaccggct tagataacca acaaattttg aagaatattc gtcgcttttt aattgagtat
     1201 cctaactgcg aagtgcgaat tcgagttcct gtggttccag gtatcaatat gaacctgacc
     1261 gagctgctga cgattgcttg ttttgtggca gatcttgatc ggttcgtacc gttagaatta
     1321 ctaccgtacc actgctatgg tatgcacggt taccaagcat tgggactgga gtatccgttg
     1381 gcagacacgc cagcgccttc acctaaagaa atgtttgcat tggcagatca actggctcgc
     1441 acggttcctc atttgctggt cacagcttta accaaaacat ttatttattt attgaatcca
     1501 agctttatct tatggataaa tgaaagacac tcactagctg cagccagctc aagaatagag
     1561 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
     1621 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
     1681 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
     1741 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
     1801 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
     1861 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
     1921 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
     1981 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
     2041 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
     2101 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
     2161 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
     2221 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
     2281 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
     2341 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
     2401 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
     2461 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
     2521 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     2581 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     2641 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     2701 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     2761 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     2821 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     2881 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat
     2941 tatgattcgt catggcaaa
//
LOCUS       DAZBWR010000187         2959 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000187 DAZBWR010000000
VERSION     DAZBWR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.192"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..1611
                     /locus_tag="ACHGT5_002794"
     CDS             133..1611
                     /locus_tag="ACHGT5_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN7218720.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1679..2770)
                     /locus_tag="ACHGT5_002795"
     CDS             complement(1679..2770)
                     /locus_tag="ACHGT5_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN7218721.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         1047 a          523 c          461 g          928 t
ORIGIN      
        1 attgaaaata cttaaaatca aaaaatatct ttttaatcaa taaaaaaatt gacagtttta
       61 ttaataataa gctattctgt tatagaagaa aaaattgttt gttattatta gcaacaaaaa
      121 aaggagtgca tcatgaaaaa gttatctcaa aaacgtttag ctcaaatcat tattgattct
      181 agaaaatctc tgaaaatgac acaacaacaa cttgcagtag ctactggtat caaccgtgcc
      241 ttaattagtc gattagaatc tctcgatttt atcccgtcaa tacaacaatt agaatcatta
      301 gcagaagttc taaattttga accaacagat ctatttataa atgaatcaaa taaatcaaac
      361 agaacaaaac atcagtcgaa gaatattgct gtagccggta ctggttacgt aggtctttcc
      421 attgctactc ttcttgccca gcacaatcag gtaacagcag tagacattct tcctgaaaaa
      481 gtaaaacaaa ttaacaataa gaaatctcca atacaagacg attacattga aaaatatcta
      541 gctgaaaaaa atcttaattt aattgcaacg actgatggtg cagccgccta tagagaagcc
      601 gattttgttg tcattgctgc tccaacaaat tatgatagta aacaaaatta ttttgatact
      661 tctgctgtag agtcagtaat caaattggtg ttagaagtca atccaaaggc aataatggtc
      721 atcaagtcga cggtacccgt aggctatacc gaaagtattc gtaaaaaata caatactaaa
      781 aatattctat tcagcccaga atttctcaga gaatctaagg cattatatga caatctatat
      841 ccttcgcgaa ttattgtcgg cacggaccaa actgacaaaa ctttacgaga agcctctcac
      901 acttttgcag atttactcca agaaggagca atcaaaaaag atatcgaaac tcttttcatg
      961 ggattcacag aagctgaagc agttaaatta ttcgctaata cttaccttgc acttcgagta
     1021 tcttacttca atgagctgga tacttatgca gaaatgaaag gactaaatac acaagaaatc
     1081 attcacgggg tttgcttaga tccaagaatt ggcacacatt ataataatcc atcttttgga
     1141 tatggaggct attgtttacc gaaagataca aaacaacttc tcactaacta taacgatgtt
     1201 cctcagaata tgataagtgc aattgtcgat agtaatcgta caagaaaaga ctttatagca
     1261 gatcgagtgt tagaacttgc tggcacgtat gaggctaaca gttcgtggaa tgcagaaaaa
     1321 gaaaaagagg tagtcatagg tgtataccgt cttacaatga agagtaattc agacaacttc
     1381 cgtcagtcat ctattcaagg agttatgaag cgaattaaag caaagggggc aactattgta
     1441 atttatgaac caacacttga aaataacact acattttttg gttcaaaagt aataaataat
     1501 ttagatgatt ttaaaaatat cagtcacagt attattgcta atcgttacga tagtaatctt
     1561 gatgatgtaa aagaaaaagt atataccaga gatttatttc aacgagatta aaaaataaca
     1621 cctcagattt gtagtgtctg aggtgtttct ataaactttt ataagtaaaa aaatacaatt
     1681 atacttcata aaaagactta taccactgcg caaatttacg aagcccgtca cgcaaacttg
     1741 tacttggttt aaattcaaaa tctcgttcta acgcactagt atctgcataa gtaactggca
     1801 catctcctgg ttgcattgga actagttcgg tatgagattc aaagtcgtaa tcttctggta
     1861 gcacccctgc tgcaattagc tcttgttgta gaatatctac aaaatctagt aggttttccg
     1921 gcgtattgtt accaatatta taaatatgat atggtgggat tggtagccca tcttccccat
     1981 caattttctc tggagcgcac tgcataaccc tctttactcc ttcaacaata tcatctatat
     2041 aggtaaagtc acgcttgcaa ttcccataat tgaatatttg gatattatca ccagcacgaa
     2101 gtttatcagt aaaaccaaaa taagccatat ctggacgccc agcaggacca tatactgtaa
     2161 agaaacgtaa ccctgtagac ggaatattgt aaagtttaga atatgcatgt gccattgatt
     2221 cattactttt cttagttgct gcatacaacg atacttgatt gtctacttta tcctctgtag
     2281 aataaggaat tttcttatta gtaccataaa cggaagacga tgaggcatac actaaatgtt
     2341 ccacaccaga ttctccatta tcatatgaat gacgacaagc ttcaagaata ttatagaatc
     2401 ctattatgtt agattcgata tacatatctg gattagtaat tgaataacgg actcctgcct
     2461 gagctgctaa attcaccacg atacttggtt tataattatc aaagatgaaa tcaattgttg
     2521 ctttatcagc aatagaacct ttgataaaat tccaagtaga tcctggatgg ttctttgcct
     2581 ccgtttctat ctctgataat ctatagttct taatagagac atcatagtaa tcattcatat
     2641 tgtcaatgcc aatgattttt attgatgact gagtacgtaa taactcaaga accaaatttg
     2701 atccaataaa accggctgcg ccagttatta atattgttct atttgataag gacgtgttta
     2761 attccagcat tttttagcac tcactttctt tttttcttat gaatacatat ttgttatata
     2821 ttaaaaaatc ttatttagac ttatctattg tcacttttat cattctaatc tttgcacgta
     2881 gtagaataat tatacagata ggaaattgct aagcaaccca cttctttcta ttatctatta
     2941 acttattcta aaacgaata
//
LOCUS       DAZBWR010000188         2796 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000188 DAZBWR010000000
VERSION     DAZBWR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.193"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHGT5_002796"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHGT5_002796"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN7218722.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1086 a          435 c          568 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaaa aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DAZBWR010000189         2791 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000189 DAZBWR010000000
VERSION     DAZBWR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.194"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1961
                     /locus_tag="ACHGT5_002797"
     CDS             <1..1961
                     /locus_tag="ACHGT5_002797"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HFN7218723.1"
                     /translation="IGYLEDDLNKIKINSDDVMYFFMTPLSDFPSSVIDNVEGIQIYS
                     NTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQARNYYQGDDLELIP
                     KPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGKLLGKIINFSKDN
                     EDYPLNVFWENTDGSRVLFGEIIGQNNSMYGVAYRIEKDNFYKKFIDEGSKNYVGYDD
                     FINQTKDIIFVPYLLLQKIIETKKDINEGIENISDSKISPVSSIITEQTQIDISELED
                     KHAIQQNDHFSEHNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGDSLVVLAGLSGTG
                     KSQLVTSYAKALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYRPGDSGLIDALLE
                     ASRHKENIYMVCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNEDLENKLYNSSTY
                     PSTIEVGQNILFVGTINTDESTHQFSDKVLDRSNIISLELVPFHEISEVNDYIQSDQK
                     KRNQLKYDDFKNFKNSNPTNGLTQKEKEMFWKLHLAINSKDKNIGIGWRILKQIDEYL
                     KNLPVQNDLSRKEAIDIQLVQRVLSKIRGSDEQLSELVGKWNENDLKQVGEFEQIINE
                     YPESSDFKYTKKILLQKARELNLHGFTL"
     gene            1945..>2791
                     /locus_tag="ACHGT5_002798"
     CDS             1945..>2791
                     /locus_tag="ACHGT5_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218724.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNIIIYNRDQGKTVPLI
                     PGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKMVGGLARTFIPKKTSRRE
                     ITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRIDKRYNWKPIGSKNLVDQ
                     KTISQLQIHPEKRGLLYTPSRIIKYDIIENQWIKFILSYFKNFSLLADRYLEKILQGI
                     IEEKKR"
BASE COUNT         1105 a          372 c          434 g          880 t
ORIGIN      
        1 ttattggtta tttggaggat gatttaaata aaattaaaat aaactcggat gatgtaatgt
       61 acttttttat gactcctctt tcagatttcc ccagtagtgt gattgataat gtagagggta
      121 tacaaatata tagtaatacg cttactaaat ttgggtttat tgatgatata aacgaatctc
      181 atagtactcg tgcccaatca cttaaagaat ttttagatcc ttatattatc gtttgtacac
      241 caaaaattaa aagaaatcat gaacaagcaa gaaattacta tcaaggtgat gatttagaat
      301 taatccccaa accaaagtct gaagttttta aaaatttaat aaaagagaat gaatcattag
      361 tacctatacc ggtcttttct ataaataaca gcgattctgt aatcaaagac gagacaagtt
      421 ttgaaaacca attactaaac ggcaaactac tcggaaaaat tataaatttc tcaaaagaca
      481 atgaagatta ccctttaaat gttttttggg agaacactga tggatcaaga gttttatttg
      541 gtgaaataat cggacaaaac aattcaatgt acggtgtagc atataggata gaaaaagata
      601 atttttacaa aaagtttatt gatgaaggta gtaaaaacta tgtaggttac gatgatttta
      661 taaatcaaac aaaagatata atttttgtgc cctatctatt acttcaaaaa attatagaaa
      721 ctaaaaaaga tattaacgaa ggcatagaaa atatctctga ttccaaaata tctccagtaa
      781 gttcaattat tactgaacaa acgcaaatag atatatctga gttagaagat aaacacgcta
      841 ttcaacaaaa tgatcacttc tctgaacata atgaatttat agaaagattt aatcagctca
      901 caattcgtag taaattacat tactcaaaac gagacttatg gaattttcat actgctatgt
      961 tgggtgatag tttggtggta ttagctggac taagtggtac cggtaaatca caattagtaa
     1021 catcctatgc aaaagcactt caattatcag atacacaaat aaagtttatt tctgtgagac
     1081 ccttttggga ggatgattct gatttattgg gctatgctga tactgtaaat tctgtctatc
     1141 gtccaggaga tagcggtcta attgatgcac tattagaagc atcaagacat aaagaaaata
     1201 tttatatggt ttgttttgat gagatgaatt tagctcgggt tgaacattat ttctcccaat
     1261 ttctttcagt tttagaaatg gagccaaata ctaggaaaat caaactgtat aacgaagatc
     1321 tagaaaataa attatataac tcttcaacct atccttctac aattgaagta ggacaaaaca
     1381 tattatttgt aggaacaata aatacagatg aatctactca tcaattctca gataaagttt
     1441 tggatagatc aaacattatt tcacttgagt tagtaccatt ccatgaaatt agtgaagtaa
     1501 atgactatat tcaatcagat caaaagaaac gtaatcaact taaatatgat gatttcaaaa
     1561 attttaaaaa ctctaaccct actaatggtt tgacccagaa agaaaaagaa atgttttgga
     1621 aactccattt agcaattaat tctaaagaca aaaatatcgg tatcggttgg agaatattaa
     1681 aacaaattga tgaatattta aaaaatcttc cagtgcaaaa tgacttatca agaaaagaag
     1741 ctattgatat tcagcttgta cagagagtat taagtaaaat cagaggttct gatgaacaat
     1801 tatcagaatt agttggtaaa tggaatgaga atgatttaaa acaagtgggt gaatttgaac
     1861 aaataattaa tgaatatcct gagtcttcag attttaaata tactaaaaaa attcttttgc
     1921 aaaaagctag ggagttgaat ttacatggat ttacccttta gtctgaaatt aaactttggt
     1981 tataaaagtg aagaacaaga atatgaactt ttattttttc aagatgaggc atcgttatta
     2041 gagtatacac cggttaaggt aaaagaattt tctaatctaa gctttacatt taacagtata
     2101 gacaaacacg caaaactata tataccagaa ttagatgatt atttgattga aaatcaagta
     2161 aaagaggaaa atggagatat ttgctatatt ccaagtgatg ataatataat catttataat
     2221 agggatcaag gtaaaacagt acctcttatt ccaggttatt acactcttta cgttaaaata
     2281 ggtgaaacta tcttttattc tcgatttgaa ataatactta aagatttagg tacaccagaa
     2341 tgggaaaaaa tgaaaatcga aattgaaaaa atggttggcg gtcttgctag aacttttatt
     2401 cctaaaaaaa cttctcgtcg tgaaattact acttcaaatt atctaatgaa cgatcaactt
     2461 tttcaacgaa tattagagtt tgagaacaat atacctcaag caatagtttc tcttgaaagt
     2521 ttaaaaaaag aagcaaaata tagaattgac aaacgatata actggaaacc aattggaagc
     2581 aaaaatttag tggatcaaaa aactatttca cagttgcaaa tccatccaga aaaacgtggg
     2641 ctactatata cacctagccg cataataaaa tatgatatta ttgaaaatca atggatcaaa
     2701 tttatcttat cttattttaa aaatttttca cttctagctg atagatattt agagaaaatc
     2761 ctacaaggta ttattgaaga gaaaaaaaga g
//
LOCUS       DAZBWR010000190         2790 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000190 DAZBWR010000000
VERSION     DAZBWR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.195"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1491
                     /locus_tag="ACHGT5_002799"
     CDS             73..1491
                     /locus_tag="ACHGT5_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7218725.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            1503..>2790
                     /locus_tag="ACHGT5_002800"
     CDS             1503..>2790
                     /locus_tag="ACHGT5_002800"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN7218726.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAA"
BASE COUNT          898 a          438 c          592 g          862 t
ORIGIN      
        1 gtgaatataa aaacaagaaa aaaagagtta taattttgaa aacgtttact agaagaggta
       61 ggaggaattc aaatgctttc acaaaatgct gttacagcaa atacaataaa aaaagaaaaa
      121 aaggaatttg ggatttccga taagataggc tacatgttcg gtgatctagg taactgtttt
      181 attttaggtt tagtcaatag tttcttgatg atttattata cgaatgttct tggcgtttca
      241 ggggcaatcg taggtacgtt attcttgatt tcgagaattt tggatgcgtt tgctgatgtg
      301 atggttggaa gattatgtga tgtctcagag ttaacagaaa ctggccgatt tattccttgg
      361 atcagaaaaa tgaagtatcc attttgctta gtgactgtca ttttgttttt gccattcgtc
      421 caacatcttc caatgacggt gaagattgct tatatttttg tttcatacat tgtttatggg
      481 atttttctct caacaatcaa tatcccgtat ggagcaatgg cagcagcaat tagctcaaat
      541 cctaatgacc gcacctcgtt gtccacgttc cgaagcattg gttctgcgat tggcggatca
      601 actacaggat tcttcattcc gattttaatg tatacccata acaagagtgg acaacaagtc
      661 gtttcaggga atcatttctt ttggatttca atcggatgtg ctgcaatagc atttgtcgca
      721 tatatgttaa cttgtaaatt gactactgaa agagtaagag ttgtcaaaaa cgaaaaagta
      781 tctgcttctc agttggtaaa aggtttgtta gctaatcgag cactgattat tttagtcatt
      841 gttgacattt tcattgtgat caatcagatt ttagctggga cgaatatgac ttatttattc
      901 aatgattact tccataataa acaagcaatg tctgttgctc ttttatttac ttacggaaca
      961 gtagtagttt tagcaccatt tgctacaaag ctgacaaaat tatttggtaa aaaggaagca
     1021 agcgtatttg cgttacttgc ttcttcatgt atgtacttgc tgatgtattt ccttcatata
     1081 acaaatgcat gggtatatct gattctttta tttatcgcta ctttaggatc tggtttattc
     1141 aatttgatgg tctgggcatt cattactgac gtgattgatt atcatcaata taagacaggc
     1201 ttaagagaag atggaacagt atatggagta aattcttttg cgagaaaagt aggacaagca
     1261 tgtgcaggag cggtaggcgg atttatgctg gcactcattg gttaccaatc gtctacaaca
     1321 ggtggagcca tccaatcagt tttagtacaa gaaaggattt atacgatggc aaacttgtta
     1381 ccagcattct gcttatttgt atccgcaatc attttgctag taggttaccc actgaatcgt
     1441 tcaaaaactg tatttattgg tgaagaattg aaaaaaatca atgaaagata gggagaaacg
     1501 tattgcggac aaaaaaaggg attatcaatg ctttgaactt taaaacaaac gacactcaac
     1561 aagatgatac aataatactg caaaaagcaa tcgatcaagg tgcgaaagaa cacttacctg
     1621 tatttattcc aaaaggcatt tatttagtcg gagccctttt tttaaaagat aagagtcatc
     1681 ttatctttga agaaggtgca gtcctcaagg ggagaacaga tatcgaagca tttccagaaa
     1741 tcgatacacg agtagccggc gtagaaatga aatggccagc agcgattttg aatgttctct
     1801 cggcgaaaga tattcttata gaaggaaaag gaataatcga cggtcaagga gaccattggt
     1861 gggagctgta ttggggaaaa gatcaaaaaa gcgggacaag agctgaatac gatcaaaagg
     1921 gacttcgttg gattgcggat tatgctatta aacgtccacg cgcttgttta ctgtatcatg
     1981 cagaacatgt tgttgttcgt gatttgattt ttcagaaatc aggtttttgg aacttgcaaa
     2041 tcacttattc taacgatgta ttagtagaaa aagtaatcat ccgcaacaat gatggaccaa
     2101 gtacagatgg gattgatatt gattcttcaa caaatgtgcg tgtctatgaa tgtgatttgg
     2161 cctgtggtga tgattgtatt gctattaagt ctggcagaga tggcaatggt gcaagagtca
     2221 atcgaaaatc atcaagaatc gaggttgcta gatgtaaaat ccgttcagga tacggtgtaa
     2281 ctattggtag cgaagttagt gctggagtta gtgacgtata tattcacgat atcgattttt
     2341 ttcagtcaga ctgtggtatt cgaatgaaat cttcaaaaga aagaggggga gtgatcgaaa
     2401 atatcagggt agaaaatctg aatatgattg atgtacaatt tcctttttct tggataatgg
     2461 actggcataa tgaatacaat agaaaaaata gcaatgacct tgctcaaatg ccagaaagtt
     2521 ggcaagcagt ggctgaagaa atacctgaat caaagcaaat gacgaaagtc aaagacatac
     2581 acatcaaaaa cgtacaggca tgcctttctc caggttactc acttccttcc cgagcttttg
     2641 atttagtagc atttcctgaa aagcctatag aagatgtctg cttttctcat tgccaaataa
     2701 ctgcaaaaga attcgggcga atcgaagcag tacaaaactt atgtttcaaa agctgtgatt
     2761 tatctataga aactggcaac acagctgcaa
//
LOCUS       DAZBWR010000191         2778 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000191 DAZBWR010000000
VERSION     DAZBWR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.196"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHGT5_002801"
     CDS             <1..963
                     /locus_tag="ACHGT5_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN7218727.1"
                     /translation="PINTFAYTINNEFNLGPNEGSSQVANNQYILLHETANETATGRN
                     EAQYMKRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            1267..1674
                     /locus_tag="ACHGT5_002802"
     CDS             1267..1674
                     /locus_tag="ACHGT5_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218728.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1688..2089
                     /locus_tag="ACHGT5_002803"
     CDS             1688..2089
                     /locus_tag="ACHGT5_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218729.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2091..2462
                     /locus_tag="ACHGT5_002804"
     CDS             2091..2462
                     /locus_tag="ACHGT5_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218730.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(2498..>2778)
                     /locus_tag="ACHGT5_002805"
     CDS             complement(2498..>2778)
                     /locus_tag="ACHGT5_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN7218731.1"
                     /translation="LWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYF
                     KIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         1010 a          425 c          504 g          839 t
ORIGIN      
        1 cccattaata cgtttgctta tacgatcaac aatgaattta atttgggccc aaacgaaggt
       61 agctctcaag tagcaaataa tcagtacatt ttactgcatg agacagctaa cgaaacagca
      121 acagggcgca atgaagcgca gtatatgaaa cgttcatgga ctagtgctta cactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc taacagtccc gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tagctagaga ttcagcaatg
      361 aaatatggta ttccacttac actagacaca ccgtataatc aaccagggat caaatcgcat
      421 ttatgggtaa cacaaaatat ttggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcctat gatttggctc atggatttac cgatgaaaat
      541 ccgacaactt cagatgataa accagtcatt gatccaactc gagcaggtgc tgcaaatcct
      601 accctgacag atggaacaaa ttacgcccac attgatcagt tcggagaaat cgaaaacgca
      661 aacttacatg tggctggttg gcacatcgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaaaagctg atggaatata tagaccagat
      781 gtaaatcaag cttataatac tttaggaaat gttggctatc atgtatcttt taacatgcgc
      841 aacttcccta ataagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agaagggaac
      901 actaaaggcg gagcacaaga ttttcatgat aaacgctggt atttaaatat tccgcaacga
      961 taaaaatagc ccctcatttt tgaggggaag tacatatagt tgttgatata gttgctaata
     1021 ttgttagcta atttgttagt aaaactgtat acgaatttag atagtaaaaa aacagtttgt
     1081 tccttatata gaggcacttt tactactgat taaatgtcag atatgcttac aaatatactt
     1141 ataaatatgc ttacaaaatt agctaatatt tccgttgaca aagctttgtt actattgtta
     1201 aactaaattg actagcaaaa gtgaaacata aatttattaa ataaatgtaa aaattaggag
     1261 aaatcaatgt cttatagagt acaattaata atttcagaag atgtagaaag tcagcaattt
     1321 ggcactaatg ttattaataa agtaattaat ccagctcttt ctattaatgc gcctttaatt
     1381 cctactgctt tatcattttc tgtaacagct gtggtgtctg agatagagga tacagaaaag
     1441 ataaaaatag tagaaattga agttttaaac aagaatgaaa aacaaatttt ttctacgggt
     1501 gaagtatcgg taagcttgcc tcctcaagtt aacgatataa actttaatat aaacgccaga
     1561 aatgtcttgg ttgaagaagc aggagagcat tatgctgttg ttaaatttaa tggaactgag
     1621 attggaaggc aaatctttga tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac
     1681 gatacggatg cttgatatag taccatcaaa tacacatcgg agtggtagct taataaattt
     1741 tgctgcgatt tttgcttgcg catcggtaat tctagcatct ccagttgttg tggatgcaag
     1801 ttctactccg aaaactaaaa atgataatca acttgtaatt actacaaaaa atgagattaa
     1861 cacaatttca agtaatgatg gcaaaatttt tgatgtgata aatacggttt taaaaagaaa
     1921 agctgaaatc aatataaaat atgatgaaga tttaaacttg tatttctttg ttatcaaaac
     1981 aacatctgaa ttgttttcct cagattacga tgttttggat actctcgata atgtattatc
     2041 tgactataaa tatatgggga aatctgtagt agcaactttg ggggaataaa atgtttgact
     2101 ttaatgaata tttatctatt tccaaacaaa ttgaatcaga tgataaatat aattctaaag
     2161 aatcttatag aagaactgct gtttcaagag catactacag tgcttttaaa aaatctgatg
     2221 agtatttgaa agaaaattat gatattattt ataatggaag tagcggaaaa ggaagccacc
     2281 aaaccgtttg gaatttgttt tcaacagtta aggaattgaa taccttagga atacataata
     2341 gtgggtatag aatgttagag aaaagaaaat gtgctgacta tattcctaat gaaaccatta
     2401 cgaaaacaga tatggcgctg atgaatcgag aagcagaaaa aataataaac aaactcacct
     2461 aatgaatttc aagcgagttt gtttgttatt tttttattta atctttgtat ctttctggat
     2521 caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacgt
     2581 cagtcacgat atcaaacaaa aagtatacat ccttcttcat tctagttttc gcagcaggaa
     2641 ttttgaaata gttcttatta gaatagtaga gattgattaa taagctatct tcgattgcta
     2701 aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat
     2761 cgaatttaaa ccataatt
//
LOCUS       DAZBWR010000192         2689 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000192 DAZBWR010000000
VERSION     DAZBWR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.198"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..296)
                     /locus_tag="ACHGT5_002806"
     CDS             complement(<1..296)
                     /locus_tag="ACHGT5_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN7218732.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENEL"
     gene            complement(314..634)
                     /locus_tag="ACHGT5_002807"
     CDS             complement(314..634)
                     /locus_tag="ACHGT5_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7218733.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            928..1068
                     /locus_tag="ACHGT5_002808"
     CDS             928..1068
                     /locus_tag="ACHGT5_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218734.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1366..1623
                     /locus_tag="ACHGT5_002809"
     CDS             1366..1623
                     /locus_tag="ACHGT5_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFN7218735.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1594..1902)
                     /locus_tag="ACHGT5_002810"
     CDS             complement(1594..1902)
                     /locus_tag="ACHGT5_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218736.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1980..>2689
                     /locus_tag="ACHGT5_002811"
     CDS             1980..>2689
                     /locus_tag="ACHGT5_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN7218737.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEG"
BASE COUNT          957 a          467 c          462 g          803 t
ORIGIN      
        1 agttcgttct caatatagat atttaataaa attagaatag ctattttatc tgcttctgtt
       61 tcaaattttg agtgaaaagt tgtagaagta tcgtacaaaa ctgaaaattc aaaatgagag
      121 gcgctgaaat gtgcaagttc atgagataaa tgaaaggctt ctgcagtttc gccatataga
      181 ttttcattca aaaaaatgat tctaggcttc ggataataga agccaggctc tttcatttcc
      241 atgtaaacta ctttcaaatt gtattcgctc aacatttctt tcaatttcaa atacatacaa
      301 cccatcactc caactattca ttttcctcta aagctttagc gattgcaatc gctttacgca
      361 ttgtctcctt agatatttct tttccatcaa aagaaaaaac agtatcgttt tctgataaat
      421 ccacatgttt aggggtttct ttttcttcgc gccccagaag atagtctaca gagacatcga
      481 aatagtcagc aatttcttgc aaacgttctg tagaggggtt tgagttctta agtctatata
      541 agacgttttt accatagcct aggtcttctt caactttatt taatgatttt ccctgttttt
      601 tcgctaactc ttttattctc tcaaacgtta tcatatcaat attcctttcg ttcaagaaaa
      661 aatatttaac tttatttgtt aaaattcgtt gactatttta acaaaaggtg ttaatatcat
      721 tcttgtaaac aagtttaaca actaaaaaga caacaaaaaa cactattgat taataaatgc
      781 caaccgccaa gaaagctttt aaaatcaatg tttatatgtc ttatttaact atgttctgat
      841 tttaacactg tatgttaatt tagtcaacgc agttttttaa aaagttgtta aatttgttta
      901 cgaatataaa agaaaggaga gaagaatatg gaaaaaacag tctcggcaaa aatcgaagat
      961 ttgaaattag acatactgaa acaagcaaaa gtggcgatgg aacacgcggt agataaagaa
     1021 gactctgcca tggttgcagc catagcagag attttagctc acgtttagtc atcgttttta
     1081 cggtcttctg gaagctgacc atagataaca gcataatgaa tatctaaata agtttgaaca
     1141 atttcttttg gcgaaattga ttcgccttta gtagatactt gtgattcgtg atttagccat
     1201 gcaacaacta aatccgcagc gatttcaaac ggcgttacac tttgtgtcag ttgccataaa
     1261 gaatttcaca atatgtatgg tcgtggtaat aataccaaag agcaatttga ggaatttatg
     1321 gaaattaaaa aatacaaaaa atcatttatt aaaggaggct aaacaatgaa caaggaaatg
     1381 agccaaacaa tcaaggtaca aaaaatgata gatgatctaa cacatggaat tgatagccaa
     1441 gccgataaaa tcatcaagga attgcaagga caaaaagtta aggatgcaaa aatgcttctc
     1501 aagactatca actttgaaat gaatccaaca aaaagaaagc tcgccgatgt gttggaagaa
     1561 aagttggctt ccgctatcaa cgagcaagaa ttactatttg aaaccgatac ttttaacagc
     1621 tgatgtttta tagatgacag tttcatccga atctatatca tcgccgagag aaaagaatgt
     1681 tgcttttgac aaaaagccga taagacctac aagcggtctt gatgttgcaa gagtagatac
     1741 cccaatatca ccttgcaagg tattcacgta aatttgagaa gaatagaaca ttccatcttt
     1801 ttcttcgata tttgacgcat catcgtatcc aacgaccaaa gtatcggcaa atattttgat
     1861 tttttctcca tcaagcatag taattacaaa ttctggtttc ataaattttc acctccttaa
     1921 caattatttc agcctgtcac actgataagg aaattatacc aaagaaagga atgagaaata
     1981 tgaatacacc acaaattttt aatttcgagc aaaacgaagt tcgaactatt ttagtaaatg
     2041 atgaaccata ttttgtagga aaagatgtag cagacgttct aggatattcg aatccgcaaa
     2101 aagctattcg agatcatgtg gacttggagg ataagacgca gaacgattcg ttcaccgtca
     2161 atggaacagc agttgttttg attaacgagt caggccttta cagtttaatc ttaaaatcaa
     2221 aacttccctc tgccaaaaaa tttaaacgtt gggtaacgag tgaagtcctt ccaacaatta
     2281 gaaaaacagg tagctattca aacgtacctc aaagttttgc acaagcattg cgtttagcag
     2341 cggatttaga agaaaagaac caattactcg aacaacaaat tgccgagtac gaaccaaaga
     2401 ttagctactt agatacgatt ctttcatcga cagatacggt agctacttct caaattgcag
     2461 ctgattacgg aatgtcggca attgctctaa acaaattgct aaacgagtta ggtgttcagc
     2521 ataaagttag cggacaatgg atactttacc gaaaacatat gaaccaagga tacacaaaat
     2581 cgcacacaag tgagataccg aaagccgatg gcggcactaa agttgtaatg aataccaaat
     2641 ggacacagaa agggcgagtg tttatttaca acttattaat cgcagaggg
//
LOCUS       DAZBWR010000193         2680 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000193 DAZBWR010000000
VERSION     DAZBWR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.199"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..588)
                     /locus_tag="ACHGT5_002812"
     CDS             complement(223..588)
                     /locus_tag="ACHGT5_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218738.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            796..1278
                     /locus_tag="ACHGT5_002813"
     CDS             796..1278
                     /locus_tag="ACHGT5_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218739.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1475..2365
                     /locus_tag="ACHGT5_002814"
     CDS             1475..2365
                     /locus_tag="ACHGT5_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFN7218740.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          914 a          436 c          527 g          803 t
ORIGIN      
        1 tcaacacttt attctttgaa tattatagat gtttccacct caaattcaat atctattatt
       61 ggaatcatta ttgctgcaat tattacacaa ctgtttttag tgtttggaga gaagaaaaga
      121 agtaaaaaat agaaaaacta gtgatagttt tggtgatata agaaaaacat atatgtataa
      181 aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt
      241 tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc
      301 tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt
      361 aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga
      421 tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt
      481 gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg
      541 aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca
      601 cctaacaagt gttattattt tgttagtttt attgtaaatt gaatcctgca ataatcaatt
      661 actttttcat tatttctaga ttaaatgaat aaactctcat gccattatct ctaaaaaaaa
      721 agtttttccc cctaattttt ttagatagaa aattgtatag tatgatgaga gaaaaagtag
      781 tttgggggaa cgttaatgag aagaaaagca gtaatcgatc ggctttattt atttttttgg
      841 ttttttccta tcgttttgct accttttaca tgggtaaatg ctgttgtaca aggtgcggag
      901 atattaggcc tgatccaagg aagcggattt gtggtttcta ggaattttcc agctttgatt
      961 gttttctatt tagtgataag tgtaggtctc ttattaccaa aatctctgat caatcactac
     1021 aattggcagc tgctttactt ttcttgctta ttagtattta catctacgtt ttctttgctg
     1081 cctcgcttga ttatcggccc taatggtttg agcgagagtg gattgaagga tttagcttat
     1141 tcctccatgt atttcttgac gatcaaacca tggttctatt tagtaattgc ctcatttatt
     1201 cttgcaacat ttttttactt ctggacagaa atcaggataa aaagtgaacg atctgtatct
     1261 ataaaaaata actattgatg gtggatcatc agtaaaaaaa catatgagct gcgtaaaatg
     1321 catgtttctc atatgctttt tttattatta tgtaaagaat gcgaaaaacg tttacatgat
     1381 ttttacataa cttaactata tttttacagc aaccatacga gggatttaca gtcgactgat
     1441 aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt ttactctagg
     1501 tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag atggctctgc
     1561 cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg taggttcgac
     1621 agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa atccaaatta
     1681 tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg aagctggagc
     1741 agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg atgcttcaaa
     1801 attagtggat cataaagtag cagtggtcgg gatggcacca atcgtcaata aagatacaga
     1861 tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa tcactaactg
     1921 gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa acggcagcgg
     1981 tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc agtcccaaga
     2041 acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag caatcagcta
     2101 tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg gtgtagaacc
     2161 aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac atatgtacac
     2221 gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga cagaagaaat
     2281 ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg aagtagaacg
     2341 tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag tcgttactat
     2401 taaggaaaaa tcaactatgt ttccccacat atacattttt cctttaattg tgaaaaatag
     2461 ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
     2521 attcgaaaaa aagagctacg acatagaact gtcgtaactt tttttggctc ttcgtcaaat
     2581 cgtgttggtg aagacaaatt tgaataaaac ttagaccgat atgcggcgcg ttctgtgcgc
     2641 cgcatatttt tttagttttc aaagatcaga ccttgttgta
//
LOCUS       DAZBWR010000194         2667 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000194 DAZBWR010000000
VERSION     DAZBWR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.200"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..2598
                     /locus_tag="ACHGT5_002815"
     CDS             157..2598
                     /locus_tag="ACHGT5_002815"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN7218741.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          903 a          465 c          620 g          679 t
ORIGIN      
        1 acttacttta ctaaggtatc ataaaaagat taaaagaaat ataaaaaaga gaatatttaa
       61 ggattgttaa aatttaatct tagaaaaaaa taactggaac aagagctatt tgtttttaca
      121 gcaaagaatg gtatattttt gtaggaggtg gcttacttga aagaagtcgt tatgatagat
      181 gctgcaagaa cgccgatagg aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa
      241 ttaggcacac ttgtgactaa aggattatta gataaaacaa aactgaaaaa agataaaatc
      301 gatcaagtaa tatttggaaa tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag
      361 attgctttga acagcggtct ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc
      421 ggatctggta tgaaagcagt gatcttagca cgccagctga tccaattagg tgaagcagaa
      481 cttgtgatag ccggcggaac agagagtatg tctcaagcac ctatgctgaa acagtatcag
      541 tcagaaacaa atgaatatgg tgaaccaatt tccagtatgg tcaacgacgg attgactgac
      601 gcattttcaa atgcacatat gggattaacc gcagagaatg ttgcaacaca attttctgtg
      661 agcagagaag agcaggatcg ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc
      721 gaagccggtg tattttctga ggagatcatc ccagtcaaga tttctgatga agacgtgtta
      781 tctgaggatg aagcagttcg tggaaatagt acattggaaa aactgggcac gttacgtaca
      841 gtattctcag aagaaggaac tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc
      901 tctgtggtga tccttgcatc caaagaatac gcagaaaata ataatctgcc ttatttagca
      961 accatcaaag aagtagcgga ggtcggtatt gacccaagta tcatgggaat cgctccaatc
     1021 aaagccattc aaaaattgac agatagatca ggaatgaatt tatcaacaat cgatcttttt
     1081 gagatcaatg aagcatttgc agcttcttct atcgtagtta gccaagaact gcagttagat
     1141 gaagaaaagg tcaatatata tggaggagca atcgcattag gccatccaat tggtgcaagc
     1201 ggtgcacgta tcttgactac attagcttac ggattgcttc gtgaacagaa gagatacggt
     1261 attgcttcat tatgtattgg tggaggttta ggcttggcag tcttgttaga agcgaatatg
     1321 gagcaaacac ataaggatgt gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg
     1381 cgcagtcaat taatagaaaa aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa
     1441 caaacacttt ctgaagagct ctccgatcac atgatcgaaa accaagtgag tgaagtggaa
     1501 atcccaatgg ggatcgcgca gaattttcaa atcaatggca agaaaaaatg gattccgatg
     1561 gcaacagagg aaccatctgt cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat
     1621 atctgcgcag aaacacctca gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg
     1681 gagtatcaag cagtgatcaa tgcagttaat catcgaaaag aagagctgat tctttgtgca
     1741 aatgaaagtt atccttcaat cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg
     1801 gaattcatgg ggagtttcca tgcatactta tctattgatt ttttagtgga tgtaaaagat
     1861 gcaatgggcg ctaacatgat caactcgatt cttgagagtg tggcaaacaa gttacgggag
     1921 tggttccctg aagaagagat tctattcagt attttaagta attttgctac agaatcattg
     1981 gcttctgcct gttgtgaaat cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa
     2041 caaattgcca aaaaaatcca acaggcaggg gaatatgcga agcttgaccc ttaccgtgca
     2101 gcaacgcaca acaaagggat catgaatggt atagaagctg tagtggcagc tacaggaaat
     2161 gatacacgtg cggttagcgc ttcgatccac gcctacgcgg caagaaatgg attataccaa
     2221 ggattaacag actggcagat caaaggggat aaactagttg gaaaactaac ggtgcctttg
     2281 gctgttgcca cagtaggcgg agcttccaat atcctgccaa aagcaaaagc atctttagct
     2341 atgttagata ttgattcagc caaggaacta gcacaagtaa ttgctgcagt agggttagct
     2401 caaaacctgg ctgctttgcg tgcattagtt acagaaggaa tccaaaaagg ccacatggga
     2461 cttcaagctc gttcattagc gatctcaatt ggggctattg gtgaggaaat cgagcaagtt
     2521 gcgaaaaaat tacgtgaagc agaaaagatg aaccagcaga cagctataca aatattggaa
     2581 aaaatccggg aaaaataaaa aagcaatatc tttagaagaa taaagcaaga tttacagtta
     2641 ttttatttac tgcagatctt gcttttt
//
LOCUS       DAZBWR010000195         2542 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000195 DAZBWR010000000
VERSION     DAZBWR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.202"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1217)
                     /locus_tag="ACHGT5_002816"
     CDS             complement(<1..1217)
                     /locus_tag="ACHGT5_002816"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN7218742.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFS"
     gene            complement(1351..>2542)
                     /locus_tag="ACHGT5_002817"
     CDS             complement(1351..>2542)
                     /locus_tag="ACHGT5_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN7218743.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          710 a          497 c          335 g         1000 t
ORIGIN      
        1 gaaaacattg ataatagtcc agataaatct tcttgactac aaagttgtac acctactatt
       61 ctctttgtat ttttttcaaa gctaattctt aattttacac gactattttt tctcaaaaaa
      121 acaggtctaa tgaaatcttc tatttctata taatctatag actcacttaa taatcgtgct
      181 gtttttaaat tcaaacctgc tgcgtaaaaa ttgtaattaa atatagaaaa gccatttgta
      241 aattgggaac cagaaggtgt aaaatcttga ttgtcaatat gttgagttgc aatataagct
      301 gttcttagag cattagaaat agaaaaatca acactacttc tattaatgga cgtaaaaaaa
      361 gttgtagcac aatccccaat agcatatata tcttttatat ttgtttgctg gtattcatta
      421 gttagatatg ctccactaac atctctcaag agttcctttt tgccaattgg actattagat
      481 agaaatccta tagcttgtac gaccatatcc atagagtatt gggtaccact tgtagtgact
      541 attttttcaa tcttttttct tcctaaatat tttttaggac tttctgatag acaaagatgt
      601 attccatttt ttgatagtat ttcttcaact ttatctgaaa aactacggtc gtaataagtc
      661 gacaacactc tatctgcaac atcgaaaaga tatacttctt tccctctttt tcttatggct
      721 tcagcaattt caactccaat atatcctgct ccaataattc ctactttttt aatagtatta
      781 tcatctaaag ctttaaaaat ctctttagca tttggaaaat tctttatcga aaaaatatta
      841 gctaaattat tccccggtac atctaacata atttgctttg aacctgtagc tagtataagt
      901 ttgtcatatt cttgggaaaa tcttgtgcca tcttttttta cacaaataat ctttttattt
      961 tgaatatcaa ttttgtcaac tgctgtctcc aaaaacaact tttttatctt agaatttagt
     1021 tctcttctat tttgataaaa aagatcttct aaattgctaa cttctctttt tatgaacaac
     1081 gatgtcccac attctaagta actaatttct gattttttat ctatcagaat aacttcataa
     1141 tttgaatcac ataagtttag aaatcgagct gcagctagcc ccgaatggtt tgatccaata
     1201 attactattt ttttcactta atcacattct tttcccttct atgaaaagta tcttcatttt
     1261 taactaacaa cctatctccc aaaataaaaa acaaaaagag gaagaaaaag attcttcctc
     1321 ttttcagaga agatttaaga aattgatttt ctattcattc tctttttttc gatttttata
     1381 ccctaataac cctaaagttc ctaacaggat taaaccacca ctaatagtaa agatattact
     1441 atcaatctct cccgtcttag gataagcttt tgaggactgc ttttgaggac tgcttttgcc
     1501 tctatttttg ttttgcttgc taccttgatt gtcagtctta ttatctttac taacggcttt
     1561 ttcactgtct ttttcgtttg aagaaacaac tacattagta ggttcactag tattaccaga
     1621 ttcatcagtt gcagttacta tcacttttgt tcctgcctct aatttaggaa ctttaactgt
     1681 aaaattacca tttttatcag ctgtcccatg gtattgactc ccgtttggta atgtaactgt
     1741 cacctctgca tttggttcag ttttaccagt aacaatttgg tcaccctctg taataggatc
     1801 aacttgaggt ttattcggtg cttttttatc ttctacagaa atagtaacct ctacttcttc
     1861 tttagatcca tctggatatg ttactactac aatccctttt ttgtttccag ctgtactaac
     1921 atctactttc tctttccaag tataagtagt tccttttggt agatcttctt tgttcttaat
     1981 tccctcagat gcgtctggct ctttatttaa gtctgtagtc actttttgtc cttcaggatt
     2041 gtatttatcg gcatcggaac gattgtctgt cacttctact ggaactttca ctgtatcttt
     2101 cgtaccatct ggatacgtta cttcgataac cccttcgtaa ttacctggtg tgttggtatc
     2161 aatcgtacca tcaggagtga catctgttac tgttgttcct tctggtaatg ttggcaagtt
     2221 agtaacatta tctgttagat ctactgtgcc tccaacttct actttttcgc cttctactgt
     2281 tggctcatat ttatcggcgt cagaacgatt gtctgtcact gtaacatcta cttcaacttc
     2341 atcagatgat ccatctggat atgtcactac aacagtccct tttttatttc cagctgcgct
     2401 aacatctact ttttctttcc aagtatactt agtatctttt ggtaaatctt ctttgttctt
     2461 aattccctca gatgcgtctg gttccttatt caattctgta gtcacttttt gaccttcagg
     2521 attatatttg tcagcgtcag at
//
LOCUS       DAZBWR010000196         2541 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000196 DAZBWR010000000
VERSION     DAZBWR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.203"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..983
                     /locus_tag="ACHGT5_002818"
     CDS             123..983
                     /locus_tag="ACHGT5_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFN7218744.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            995..1822
                     /gene="panB"
                     /locus_tag="ACHGT5_002819"
     CDS             995..1822
                     /gene="panB"
                     /locus_tag="ACHGT5_002819"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFN7218745.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1837..>2541
                     /gene="panC"
                     /locus_tag="ACHGT5_002820"
     CDS             1837..>2541
                     /gene="panC"
                     /locus_tag="ACHGT5_002820"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFN7218746.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYN"
BASE COUNT          978 a          275 c          509 g          779 t
ORIGIN      
        1 aatatgggat attatgataa attaactatg gatgatattt gaaaaaaaca tactgtatat
       61 ttgatttaat ttttattttt aagctcatat cgtaagaatg ggacttgtag tctcacttct
      121 ttatggaggt gataattatg aacgttggtt ttattggagc cggcaaggta ggttgttcat
      181 tcgggaagta tttccaggaa cataaaatac aagtaacagg attttatagt aaaagcgaag
      241 actcgtcatt agcagcttcg aattttacat ctagtaaaca atatttaaac ttaagagaat
      301 tagtagatga aaatgatact attttcatta cgactcctga tggtcaaatt caggaggtat
      361 ggcaagaaat taaaaattat caaatcaaaa acaaattaat ctgccattgc agtggtgcaa
      421 tatcttcaga tatcttttca aatatacagg actatggtgc ttatggctat tctgtccatc
      481 ctatgttttc aatatcagat aaatataatt catacaaaaa attaaaaaaa tcttttataa
      541 caattgaagg cgatgaaaaa tatgcaaagt ttttatgtga tttcttcaaa aagttaggta
      601 atagtactgt aattgtatct aaggagaata aatctcttta tcatgccgca tcagtagtat
      661 ctagtaacct tgtgctagga cttattaata caagcgttac atatctaatt caatgtggat
      721 ttactgaaaa aatggcaatt gaagctttat atccattaat tgaatttaat attagaaata
      781 taaaagaaaa aggaattata gaaagtttga cagggccagt tgaacgttgt gatatttcaa
      841 caattaaagg tcactgtgaa gttttatcag atgaagacag agctttatat atgctattat
      901 caaaaaatgt attagagata gctatgttaa aaaatattaa tagagattat agtgaattag
      961 aaaagtattt aggagagagt taggagagaa atagatgaaa aatacagcag ttacatttaa
     1021 agaatctaaa ttaagaaatg aaaaattaac aatgcttaca gcttatgact attcaacagc
     1081 aaaaataatt gatgaagctg ggataaatgg aatattagta ggagattctc ttggtatggt
     1141 atgtctagga catgaagata cactttcagt aacaatggaa gacatgattc atcatactag
     1201 agcagttaca agaggagcta agaacacttt agttgttgca gatatgccat ttatgtctta
     1261 tcaaacttca gtatatgatt cagtagttaa tgcaggaaga ttaataaaag aaggacgagc
     1321 tcaggttgtt aaattagaag gagggataga agtatgcgat aaaatagagg ctatagtaaa
     1381 ggcttctata cctgttatgg cgcatatagg tttaacacct caatctgtta atgcatttgg
     1441 aggatttaag gtacaaggaa aggacaagga agctgctaaa gaattaataa gagctgctaa
     1501 agctgtagaa aaagcaggag cattcgctgt agtgttagaa tgtgttccaa ctaagcttgc
     1561 agagttaatt tctaaagaga tatcaatacc tacaattggt attggagcag gtgcagggtg
     1621 tgatggacaa attcttgtat atcaagatat gttggggatg tatagtgatt ttacccctaa
     1681 atttgtgaag aaatatgcta atttatcaga agagatgaat aaagcattta caaaatatat
     1741 agaggaagta aaagatggag tttttccagg tccagaacat gggtttaaaa taagtgatga
     1801 tgttttagaa aaattatatt aaaagggtca agaaatatgg ttatatttaa aacagtgaat
     1861 gatgttagaa gtcaagtaaa agagtggaaa aaacaaggct taaaggtagg attagttcct
     1921 actatgggat atttacatga aggtcatgaa agtttaatta gaaaagcatc aaaagaaaat
     1981 gataaagttg ttgtaagtat ctttgtaaat ccaactcagt ttgggaaaaa tgaagattta
     2041 ggtagttatc caagagactt agaaagagat atagaagtat gcacaagagg tagagcaaca
     2101 gcaatattta atcctgaagt tgaagagatg tattgtgaca atgcaagtac ttttgttaat
     2161 ataactggat taacagaagg tttatgtgga gctagtaggc caatacattt tagaggggtt
     2221 tgcactgttg taagtaaatt atttaacata ataccagcag atagagctta ttttggagaa
     2281 aaggatgcac aacagttagc tgtaattaaa agaatggtta gggaccttaa tattgatata
     2341 gacgttgtag ggtgtccaat tataagggaa gaggatggac tagctaaaag ttctagaaac
     2401 acatacttaa gtttagagga aagatcttca gctactattt taaataaatc tttaacttta
     2461 gctaaggaag ctttaaataa tggagagaga gacagcttaa aaataattga gataataagt
     2521 aaaaatataa atacttataa t
//
LOCUS       DAZBWR010000197         2438 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000197 DAZBWR010000000
VERSION     DAZBWR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.204"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..2213
                     /locus_tag="ACHGT5_002821"
     CDS             60..2213
                     /locus_tag="ACHGT5_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN7218747.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          835 a          430 c          545 g          628 t
ORIGIN      
        1 ttattaagaa gtttttgggt acaatgaagc atgaatgtaa ataggcggaa ggataggaaa
       61 tgatgaaaga ttttatgaaa aaaatattaa atacttcttg gttagaaaaa ataagaaaag
      121 gtgcacaaga acctaagaac aaaacgtttc gccggtccca tgttcctttt cgcttaaatt
      181 ttcttttttt cattatattt acgttatttg ttgcgttgat tgctagattg ggttatttgc
      241 agatcgtgaa cggcgaagag atggaggcgc gagtgaagtc tacttctacg attacggtcc
      301 aagaaagtac tcctcgcgga atgatctacg actccacagg aaaagcttta gttacgaata
      361 aagcgaatca ggccatcaca tatacgagag gaacacaagt tacggctaaa gatttgcttg
      421 aaatcgcaac gaagctgaac tcgctgatcg atgttccggt agatgaaaat ctgacagata
      481 gagataaaaa agatttttgg ctggctgatc ccaaacattt gaaagaagcc acaaaaagac
      541 tttcggcaaa agaaaagcaa ttagggaaca gcgaacaata tagtgcgatg gtcaataaag
      601 tcacagctga tgagattaat ttaaacgagg agcaattaaa aattgctacc atctttaaac
      661 ggatgaatag tgcatatgaa ttaaacactg tctatatcaa aaactctggt gttactgatc
      721 aagagttagc tgttgtggca gaacgcgctt ctgaacttcc aggagtttcg actggcacag
      781 actggacacg agaatataat gcagcaagtt cactgaaaag tattcttggc agtgtgacga
      841 ctgaaaagca aggacttcca gcagatgaag cagagaaata cctagcaaaa ggttattctc
      901 ggaatgaccg tgtaggacaa agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa
      961 ccaaaaccca atatgaagtt tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa
     1021 tcttctctgg ggaaaaaggt tctaatttaa tgttatctat gaatgcagaa ttccaatcaa
     1081 aagtagaaga aattttgaaa aggaattatc aaacattgat caacaacggc aaagcacagt
     1141 attcgcctgg tgcttatgca gtggctatga atcctcagac aggagaagtg ctagcaatga
     1201 ctggtttcag tcatgaacaa ggttccaaag aaatcaccga gaatgcttta ggcacgatta
     1261 ctagcgcctt tgatccaggg tctgtagtca aggctggtac tttgacagca ggctgggcaa
     1321 gtaacgcgat cagcggaaat caagttttga ttgatgagcc gattcgtctg caaggtgctt
     1381 cagaaaaaag ctctgtcttt aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat
     1441 cggaattgtc ttctaatacg tatatgatca aagtggcgtt aaagatgtta ggactagact
     1501 atacacctgg aatgggacta cctagtttag atgaagaagc taaagcttat cagcaattga
     1561 gagactcttt caaagagttt ggtcttggta caacgactgg gatcgatttg ccgaatgaat
     1621 ctcctgggat ctcacgttct gttgattata tgaagaagtt caatgcggat aatggcaaag
     1681 aatggtatgc tccaggtaac tttaccgatt tggcatttgg acagtttgac acatatacac
     1741 caatccaatt agctcaatat gcttcaacag tcgctaatgg cgggaaacga gtacagccgc
     1801 acctagtcaa agcgatctat ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag
     1861 aaattggcac aacagtagaa aatacggtca atatttctgc agaaaatatg gcgatcctcc
     1921 gtgaaggatt ccaccaagtt gtccatggta ccgatcctta cacaacagcc aaaccattag
     1981 ccagtgcgaa aatggacctt tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc
     2041 atccagatat tgcgacagtc aatagtaata tcgtggcata cggaccaaca gacaatccgg
     2101 aaattgccat cagtgttgtc ttgccgaact tattagacga acaagaccac ataaacttga
     2161 cgattgctaa agaaataatg gacgcgtatt atgatatgtt catggcaaaa taaaaaagag
     2221 gtcgtgaaaa agccgcttag tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca
     2281 catttttgct taaatttgac ttcataccga gaaactggct tttgaacacc gtttactcgg
     2341 caaaaaaggt tgtgacaaaa attgtggctc tttgtcaata aggactgatg agttgtgcaa
     2401 aatcaaatct gagtcagaat gaaccacatt ctggctca
//
LOCUS       DAZBWR010000198         2436 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000198 DAZBWR010000000
VERSION     DAZBWR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.205"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2436
                     /locus_tag="ACHGT5_002822"
     CDS             <1..>2436
                     /locus_tag="ACHGT5_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN7218748.1"
                     /translation="IFYLFLMGYGLKEWNWKEKVRYKLPQVFISLIVLFGLIILMTQI
                     SLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLSDKLGIKGRGNFLAVFLIVTQV
                     ICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICYLLAVSLLVIFLFYIQKIRFIH
                     RFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAFFYCFTFVQIFVLEPKEGWKET
                     IQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFWYALTATLIINLLLTISTVIKM
                     EMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLILLLLLITSIIVQRRLQKQYSLK
                     IHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQ
                     FLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLNNQTIVLNLSESFSD
                     PARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWEGLTGLSISNLSASL
                     VTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKFGFDRFYYLDSPDKL
                     TYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHMPYDDYYSELNYTAE
                     GTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVFYGDHLPAIYSGNDS
                     KKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEHANIKVTPYYALLTE
                     VSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQKEILEDYRLIQYDLV
                     AGNQYSATWATQK"
BASE COUNT          803 a          433 c          415 g          785 t
ORIGIN      
        1 gattttttat ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt
       61 ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt
      121 gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa
      181 accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct
      241 atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac
      301 tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga
      361 atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta tttgctattt
      421 attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca
      481 tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa
      541 aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg
      601 ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca
      661 ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat
      721 tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc
      781 taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga
      841 agagcctgtc tttccttcag atttaaaaat actgaccgga ttgtcagaac tattatcgat
      901 ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag
      961 tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg
     1021 atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa
     1081 aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca
     1141 gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa
     1201 aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta
     1261 caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat
     1321 cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc
     1381 tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt
     1441 atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag
     1501 tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa
     1561 aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc
     1621 tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt
     1681 agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga
     1741 tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacagaaa cgtcacaatt
     1801 catccaatta tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa
     1861 ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat
     1921 gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat
     1981 cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa
     2041 tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa
     2101 gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg
     2161 tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga
     2221 agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt
     2281 taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac
     2341 tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg
     2401 taaccagtac agtgcaacat gggcgacaca aaaaat
//
LOCUS       DAZBWR010000199         2416 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000199 DAZBWR010000000
VERSION     DAZBWR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.206"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1147
                     /locus_tag="ACHGT5_002823"
     CDS             2..1147
                     /locus_tag="ACHGT5_002823"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN7218749.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="ACHGT5_002824"
     CDS             1318..1776
                     /locus_tag="ACHGT5_002824"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN7218750.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="ACHGT5_002825"
     CDS             1935..2405
                     /locus_tag="ACHGT5_002825"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN7218751.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          807 a          454 c          455 g          700 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaa
//
LOCUS       DAZBWR010000200         2400 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000200 DAZBWR010000000
VERSION     DAZBWR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.207"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHGT5_002826"
     CDS             207..2069
                     /locus_tag="ACHGT5_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN7218752.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHGT5_002827"
     CDS             2069..>2400
                     /locus_tag="ACHGT5_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN7218753.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZBWR010000201         2366 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000201 DAZBWR010000000
VERSION     DAZBWR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.208"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1453
                     /locus_tag="ACHGT5_002828"
     CDS             152..1453
                     /locus_tag="ACHGT5_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN7218754.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1557..1757)
                     /locus_tag="ACHGT5_002829"
     CDS             complement(1557..1757)
                     /locus_tag="ACHGT5_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN7218755.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2153..2302
                     /locus_tag="ACHGT5_002830"
     CDS             2153..2302
                     /locus_tag="ACHGT5_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218756.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          768 a          453 c          408 g          737 t
ORIGIN      
        1 ttcgtacaaa aaaaccgtct tttcttggct ctataatttc taggactgat agaaaatcat
       61 tctatcggtc ccttttgagc atgaaaaaac aaaacataca gaagctccgt tagaattgaa
      121 tcaccacaac acaatcaaag gagcgttctg tatgcctgat cttcatcata tcaaactact
      181 acttggaatc aaagataaaa acattttcat cacaaatgtg gaatcgaaga gcattaagaa
      241 tatcaagagt cttgttgttt ctgcgacgtt aagtaaagaa attcgtcgct gtcctttgtg
      301 taaacagatg aatcatgaag gcatgatcgt taagaacgga aagaaaaaat cgctcattca
      361 acttaataaa tgtgcgaacc aactgactta tcttgcttta gcgaaacaac gctaccactg
      421 tcgtggctgc cacacctatt ttacggcgaa tacctacatt gtcgatagaa actgctttat
      481 cgctaaacaa gtgcgctata aaattttaga agagctaact gaaaaacaag caatgacgac
      541 aattgctaag cattgcggcg tttcttggtc gaccgtttct cggacactcg cttctctgat
      601 ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt ttattaatgg atgaatttcg
      661 ttcactgaaa aatcaagtcg gtccctactc cttttcttgt atggatggcg atactggaaa
      721 attactggat attcttcctt cacggaagaa aaaggctctc gtttcttatt ttatgcaatt
      781 tgaacgacgc gcacgattga aggtaaaggt catcgtgacg gacatgaatg tttcttatgc
      841 ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt gtggatcgtt ttcacattgt
      901 caaacatctc attcgtaaat ttgaggatat tcgcgtacga atcatgaaaa gctttgatcg
      961 aaacgatcca acacaagcca aatattatcg acaattaaag gcgttgtctc ggcttctgat
     1021 caaacgacag gacaagcttg tttatgacaa atggaccaaa tggcgcaatt ttggttgggc
     1081 gtatctcacc gaaagtgaag tggtggatcg tctgctttcg atctctgacg aattacgaat
     1141 cgcctatttt tactatcaag aaattcttca agcgtttcac gacaaagagg cggacacttt
     1201 ctttaaactt gtaagaacga tgcccaattc cgttccaaaa gaactgcatt ctataaaaaa
     1261 agcttttata aactatgaat ccggcattcg tttagccctc gaattgcctt attcaaatgc
     1321 caaaatagaa aatctgcata cccacatcaa agcattgaaa cgagtcgctt atggctttcg
     1381 aagctttcgg aaaatgaaga ccagaatttt cttactaaat aatctgatta cctatgaatc
     1441 aaaaaacatc taaagtagaa aaggaattct accttagatg ttcttatctc actctatcag
     1501 tcctatttga caaagagccc ttttctttcg aaaagacggt taaaagtcaa tttttattaa
     1561 gctttaacaa tgtttgttgc ttgagggcca cgttgaccat cttcaacatc gtaagacact
     1621 gcttggcctt cttctaaagt tttgaagccg tcaccttgga tagctgaaaa atgtgcaaat
     1681 acatcatttc catcttcacc agtgataaag ccaaaacctt tgtctgcgtt gaaccatttt
     1741 actgtaccgt tattcataaa taaattcctc ctgatacgta tcaatgtacg ttttttcgtt
     1801 gcaataaata tttgataggc aaaaggagtt ttatctatgt agatatatcg ctcaaattac
     1861 gtttcaaaat agattactca tttaatatat catgcttttt ctaataacac aaggataatg
     1921 caatgaaata taaaattcat tgtcttctat tgtcttcgaa tcgaagaaaa agttgaataa
     1981 ccgcgattga ttcacttttt gttttcttgt ttatttgctt ttatctgcaa cttttgactt
     2041 taaaatttct taactatgaa aacctttcaa ttggtggtat acttataaat ggaatttcgt
     2101 tatcaacgcg ttcgttgcct ttcagcaagt atttaaagaa aggagaatgg ctatgacatt
     2161 ctataacaaa gatatacaat atcgagtaac cttggataca gatttgaatc tatttgtcgt
     2221 ttttgacaaa gaaaatacca atcatgtagc gacaggtgtt acgatcgaac aagcagtgca
     2281 agaattaaaa aaatctgcat aaggagtgga aaccctcata tggacgattc tcgttcttct
     2341 ctttaaacgc atctactggt agataa
//
LOCUS       DAZBWR010000202         2340 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000202 DAZBWR010000000
VERSION     DAZBWR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.209"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACHGT5_002831"
     CDS             complement(365..1657)
                     /locus_tag="ACHGT5_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN7218757.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACHGT5_002832"
     CDS             complement(1931..2191)
                     /locus_tag="ACHGT5_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218758.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DAZBWR010000203         2306 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000203 DAZBWR010000000
VERSION     DAZBWR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.210"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..1753
                     /locus_tag="ACHGT5_002833"
     CDS             140..1753
                     /locus_tag="ACHGT5_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN7218759.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          724 a          408 c          447 g          727 t
ORIGIN      
        1 aaaaaatttt taggttttat ccacaaatta tttctctata ttttctgtct attcaaaata
       61 aacaataatt ttccggcaca attatttgct gttttttacc ataacatagt acgatcaacg
      121 tagaaaagga gatgttttta tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc
      181 atttgccact cgtttccgtc gattgaatga agcgcacgaa atcattatct atgaacgcgg
      241 agaaaatatt tcttatgcaa gttgtgcttt gccttattat ttaggaggtg tgatcactga
      301 ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca acatcgatgt
      361 ctttactaaa cacgaagtaa cagcaatcga tccttctact aagcgattaa cagttaaaga
      421 cctatccaca aatgaagaaa taaaaacaga ttacgataag ttgatcatct cttctggtgc
      481 tagaccagat tatccggata ttcccggagt ttttgaagca gaaaacggct ttgtcctccg
      541 tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt
      601 cgtcattctt ggtgcaggtg ttatgggtct ggaattagct gagaatttca agcatcgcgg
      661 cttagacgtg actttaatcg atcaattgcc acaagtcgct ttcccttatg atccagaaat
      721 tgctaattta gtttatgaca aattgctgaa agaaggatta gccgttcatt tagaaacaag
      781 agttactgag atccgtgata aaggtcgaga aatagtatta tcagatggct ccgtcctttc
      841 tgctgatatg ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac
      901 cggcatacaa ttatcagata caggtcagat cattgttgat gaccagttac aaaccaatct
      961 gccggacatc tatgcgattg gcgatattat cgaaacgact agtgtagtga ctggtcagcc
     1021 gatccaaagt atgctttcca gtgcggccaa tcgtcaagga catatgctgg cagatatttt
     1081 aaatggtacg cctatgcgct atcgcggata tattggtgca ggtgtcgcaa aaatctttga
     1141 tcatacagca agttatgcgg gaatgacaga acatgaatta aaagcatcag gcataacaaa
     1201 ttataaaact gtttttatca ctccctttga ccatgcctat ttctatccag aagctacaag
     1261 attaaatcta aagctgattt ttgatgcaga tagcggtcgt attttaggtg gacaagcatt
     1321 tggagaaaaa ggtgtcgata aacggatggg agaactttct gtagcaatca ccggaaactt
     1381 gacagtcttt gatttgcctg atttggagtt gccttactct ccaccgtatt ctactacccg
     1441 tgacccgttg aatatagctg gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac
     1501 gatcaaagca agtgatatac ccgaaaacga tttgaaagaa gcgttctttt tagatatacg
     1561 tgaacctaat aaagcacctt ctggcagtat ctcggcaaca aaaaatattc caatgaatga
     1621 gttacgagat cgaatcaatg aaatcccaaa agataaaaaa gtttacatta ctttcagaag
     1681 aggattgaat acttatactt ctgcccgaat cttggcaggt ttgggtatca aagcgatttt
     1741 gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt atttttactt
     1801 gaggagtaaa aatgaaaaag tatatagggt tgttggttgg tatgtatata gaatagaaga
     1861 taaatgtgaa gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt
     1921 ttaaacattg cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga
     1981 ctctgagaca aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga
     2041 ggcatcatat taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt
     2101 cttatataat aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg
     2161 tgtgatacta ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc
     2221 atcctcgttg tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta
     2281 atttttttgt tcctatttat ttaaca
//
LOCUS       DAZBWR010000204         2303 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000204 DAZBWR010000000
VERSION     DAZBWR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.211"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHGT5_002834"
     CDS             191..445
                     /locus_tag="ACHGT5_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN7218760.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHGT5_002835"
     CDS             446..763
                     /locus_tag="ACHGT5_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN7218761.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACHGT5_002836"
     CDS             1322..2257
                     /locus_tag="ACHGT5_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218762.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          840 a          311 c          433 g          719 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct
     2281 aaacatgttg gtaaatcaac aaa
//
LOCUS       DAZBWR010000205         2293 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000205 DAZBWR010000000
VERSION     DAZBWR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.212"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACHGT5_002837"
     CDS             complement(51..1598)
                     /locus_tag="ACHGT5_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN7218763.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHGT5_002838"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHGT5_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN7218764.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACHGT5_002839"
     CDS             complement(2043..2237)
                     /locus_tag="ACHGT5_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218765.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DAZBWR010000206         2222 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000206 DAZBWR010000000
VERSION     DAZBWR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.213"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACHGT5_002840"
     CDS             75..503
                     /locus_tag="ACHGT5_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN7218766.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACHGT5_002841"
     CDS             490..732
                     /locus_tag="ACHGT5_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218767.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACHGT5_002842"
     CDS             complement(1032..1247)
                     /locus_tag="ACHGT5_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218768.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACHGT5_002843"
     CDS             1354..1668
                     /locus_tag="ACHGT5_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218769.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..>2222
                     /locus_tag="ACHGT5_002844"
     CDS             1772..>2222
                     /locus_tag="ACHGT5_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFN7218770.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQR"
BASE COUNT          730 a          330 c          449 g          713 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aa
//
LOCUS       DAZBWR010000207         2150 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000207 DAZBWR010000000
VERSION     DAZBWR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.214"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..422
                     /locus_tag="ACHGT5_002845"
     CDS             <1..422
                     /locus_tag="ACHGT5_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFN7218771.1"
                     /translation="PENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKD
                     YAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDV
                     HDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(568..1044)
                     /locus_tag="ACHGT5_002846"
     CDS             complement(568..1044)
                     /locus_tag="ACHGT5_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN7218772.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(1004..1360)
                     /locus_tag="ACHGT5_002847"
     CDS             complement(1004..1360)
                     /locus_tag="ACHGT5_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218773.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1338..1523)
                     /locus_tag="ACHGT5_002848"
     CDS             complement(1338..1523)
                     /locus_tag="ACHGT5_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218774.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1524..1796)
                     /locus_tag="ACHGT5_002849"
     CDS             complement(1524..1796)
                     /locus_tag="ACHGT5_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFN7218775.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1793..1993)
                     /locus_tag="ACHGT5_002850"
     CDS             complement(1793..1993)
                     /locus_tag="ACHGT5_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218776.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT          653 a          401 c          303 g          793 t
ORIGIN      
        1 taccagaaaa tattgaaaaa gtttctccta catttgttga tatttatact caagccacac
       61 aagctaaatc tgagcaattg gaacaaattg ctggagttgg ttatagaaaa gctctagaat
      121 ttctaattaa agattatgca atatctaaaa attcagagga tgaagataaa ataaaaaaaa
      181 tgttattagg taaaacaatt gaaaattatt tgaatgattt tcctaaactc caaagattag
      241 ccaaagctgc tacttggatt ggtaatgatg aaacgcacta tgttcgtaga catactaata
      301 aagatgttca tgatatggat gcatttatta aatcagcagc gcaatttatc gcggctgatt
      361 atgatgctga tttagctgat caatttataa atggaaataa ctttatgcca actactaatt
      421 aaaagaataa aagagccaca gataccaatt aataaaacgg ttgggatatc ttggctcttt
      481 catatttttt tgacactaat agaatatcat gctgctaaca atgtttacat agctacaatg
      541 accgaacatt tagtgaacag tgtaatttta gaataaagag agttgttctt tgtacttctc
      601 gctattgtaa aaatcaattt cgttttcttt tttcaatcgg tcagcttgtc tgtgttcata
      661 ctcatctaaa aagttcaatg tctttctaat ttctgcatgt cgctgtctga catacgatga
      721 gctataccca acagattcag caatatcttc taagctcatt tgatcaacat atttcatctt
      781 aacaatttga ttatcgatac cagaaaagct atctatcagc aataacattt cttctttttg
      841 ctcgatcaac aattccaatt cattttctat cttttggata ttctcttcta aagaagaagt
      901 tctcgaattc ttttcgatgc ggacgtttgc taaatcacca cagacccatc gattcaattc
      961 aagcttactt ttattaagat tccactttaa gtaaagaatc tgctcatcta gttcttggta
     1021 atcttttaac caatgaaatc tcacaaacgc caccccttat ggtagaatag tattgcgctg
     1081 ctatctcgaa aggggtagct tttttatttt ttcttagccg ttccattctg gattgccttt
     1141 tgttccaaac gtcgtttttt cttcttaatc tttgattttg tttttcccat acaattctcc
     1201 tttccgaaaa cacattttgt tcgattttta atactaaata aaagttggcc agaatttttc
     1261 gttcgtcttt actcatcttt ttttgaataa gattactagc ataaaaatag ttagtaccac
     1321 gaaaatgaac aggtaaatca attacgttca ccttcttcat gccagttatc agccactaga
     1381 atctttgcgt tagtgccgcc accgttttct tttttgtatt tttcaaatgc tttataagca
     1441 gctgtttttg atttatagat ttttccggaa ccgactggga acatccatgt gccctctgtg
     1501 atagctaaga tgtatacttt catttaattc acccctttaa tccccaacac tacatatccg
     1561 gacttctgca tgtaatcagt aatataagtg atctctgctt tgtatgagtc accagtgtaa
     1621 ctaaatgttg gtctcgttag tccaggtaat tcatgtttaa tgtatttttt atattcacgt
     1681 agtattaact gatcaccaac tttgaagtca cgatcattct tgcggatctc gaattgtttg
     1741 cgtccgctcg tcactgcttc aaaatattct ggtagtattt ttagttcgtg gatcattctg
     1801 tttcctcctt caaaattcgc tcatcatcaa aaagtgaatc tccacaaaat ccttctccca
     1861 aatatttatc gttcatgtat aagtcaacca aatatttgtg atctgcttct cgatattcat
     1921 taatattaac gatctttatt ttgtaaacat taccttcgtc gctcactatc ttgaattgtt
     1981 ctctgacttt caccaccgtt cctcctcttt ttgaaaaaat tttagtgttt ttgtaagacc
     2041 ccctaaatca gcttttttat ttgatataat acttgtatcg attaatcaaa tcgaaattaa
     2101 atttaaaaag gtgatttatc atgaatatca acaatttagt aaaactcgtt
//
LOCUS       DAZBWR010000208         2112 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000208 DAZBWR010000000
VERSION     DAZBWR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.215"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHGT5_002851"
     CDS             complement(<1..914)
                     /locus_tag="ACHGT5_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN7218777.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHGT5_002852"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHGT5_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN7218778.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          712 a          374 c          410 g          616 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt ca
//
LOCUS       DAZBWR010000209         2102 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000209 DAZBWR010000000
VERSION     DAZBWR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.216"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..846
                     /locus_tag="ACHGT5_002853"
     CDS             <1..846
                     /locus_tag="ACHGT5_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN7218779.1"
                     /translation="IKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGV
                     LKSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLE
                     YPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYE
                     KEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEA
                     VIESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETI
                     KSLFS"
     gene            853..1248
                     /locus_tag="ACHGT5_002854"
     CDS             853..1248
                     /locus_tag="ACHGT5_002854"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN7218780.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1245..>2102
                     /locus_tag="ACHGT5_002855"
     CDS             1245..>2102
                     /locus_tag="ACHGT5_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN7218781.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDII"
BASE COUNT          827 a          233 c          408 g          634 t
ORIGIN      
        1 ataaagccag atatggtcat tgcttatact atcaaaccaa atatatacgg tggattggca
       61 tgtcggttaa gaaaggtttc ttatgcatta aatataaccg gattaggaac ggcatttcaa
      121 aaaaaggggg ttttgaaaag tcttattgta tttttgtata gattagcagc taaaaaatct
      181 aaggtcatat ttttcgaaaa ttttgaaaat atgaaaacat ttcagacttt acaaattgct
      241 aaacaaagta aatacaagct attaaacggt gcaggagtag atgtagaaca ctttagttat
      301 ttagaatatc ctaaagatga gcaagttcat tttttattta ttggaagaat tatgaaagag
      361 aaaggtgtag atgaactttt tgaagcaatg aaaaacttat atagtgaaga gaaaaattgt
      421 gtacttgatg tccttgggta ttttgaagaa gattacggca taaaaattga aaaatacgaa
      481 aaagagggat ggttaaaata tcatggatat caatctgata ttcgtccctt tgtagaaaga
      541 gcacattgct ttgttttgcc tagttggcac gaaggaatgg caaatactaa ccttgaatca
      601 gcagcatcag ggagaccaat tattacaagt gatatcccag ggtgtaaaga agcagttatt
      661 gaatcgaagt ctggttattt atgtgaaaga aaaaatagtt gtagtctcta taattcgatg
      721 aataaatttc taaaattatc atatgagcag cgcattaaca tgggaaaaat ggggagaaat
      781 tatatgatta aaaaatttaa taagaagaaa gttgtcgatg aaacaattaa atcattattt
      841 agttaaaaag atatgttttg caaagtatgt gatagaaaag agggattctg tggcttcaga
      901 atagatatca aagaattggc aaaagtttct gatatttttt tatttactac attacaagaa
      961 ggaatgccaa gatctatgat ggaagctatg gcaagtggat tgccatgtat agcaagtaaa
     1021 atccgaggta atgtagacct tttagtggaa ggaaaaggtg gatatcttgt tccagctaaa
     1081 gatgcagaat tagtagcaga gtgtttgaga gaactaatca ataataaatc tcttagaaag
     1141 tatatgggaa atgagaacct ttccagaatt aaagaattcg aggttagtat cgtaaaaaaa
     1201 agaatcaaag aaatatatga agaagttata ggtgagagtg taaaatgaaa aatccaatgt
     1261 tttcaataat agtacctatc tattgcgttg aaaaatattt gaaagattgc ttagattcaa
     1321 taagaaatca gacatttaaa aattttgaag caatactagt tgatgacggt agtcctgacg
     1381 aatgcccaga aatatgtgac tattattccc aaatagatgg acgtttaaaa gtaattcata
     1441 agaaaaacgg tggaataata gatgcacgga aaacaggagt cactagtgct atgggagagt
     1501 atatagtttt tctagatggt gatgattggc ttgcaaatac ttatttagaa aaatatgcgg
     1561 aagtaattac taagtacaat ccagatataa tatgtagtgg gtattttgca tcacgttcca
     1621 acaaaattag aaaaagagaa ttgtcttgct cgtttggata ttatgacaaa gataaaatta
     1681 taaatttgat ttatcctaaa ttaattgagg atgaacatgg agagtatttt agaccacaat
     1741 tatgggcaaa agcttttaga actactctgt acaaggaaca acaacaagta tctgggcaag
     1801 ttagtgttgg tgaagatcat gcatgtacaa aaccatgtat ttaccatgca aattcgatat
     1861 atatgatgga aaaatgttta tattattaca gaataaacga aaattctata acaaaaaaac
     1921 cacaaccata ttattgggaa ggaccaaagg ctattggaga gcattttgaa aagcagattg
     1981 atatgaataa gtatgatttt caagatcaag tatatagaaa tgttacacac aatttattta
     2041 atgttgctgt ttcacaattt aataaacagg acaaatattt aaatataaaa aaagatatta
     2101 ta
//
LOCUS       DAZBWR010000210         2064 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000210 DAZBWR010000000
VERSION     DAZBWR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.217"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACHGT5_002856"
     CDS             complement(45..1364)
                     /locus_tag="ACHGT5_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7218782.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACHGT5_002857"
     CDS             complement(1361..2014)
                     /locus_tag="ACHGT5_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN7218783.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          688 a          312 c          161 g          903 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atgg
//
LOCUS       DAZBWR010000211         2061 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000211 DAZBWR010000000
VERSION     DAZBWR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.218"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2061
                     /gene="acm"
                     /locus_tag="ACHGT5_002858"
     CDS             <1..>2061
                     /gene="acm"
                     /locus_tag="ACHGT5_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN7218784.1"
                     /translation="SLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEK
                     KQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKN
                     LDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTG
                     DMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYR
                     GEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFEN
                     HSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTF
                     ELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELK
                     FSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDA
                     ETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNE
                     NKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPK
                     YRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTER
                     EKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVI
                     IGLL"
BASE COUNT          807 a          341 c          405 g          508 t
ORIGIN      
        1 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
       61 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
      121 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
      181 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
      241 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
      301 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
      361 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
      421 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
      481 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
      541 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
      601 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
      661 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
      721 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
      781 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
      841 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
      901 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
      961 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
     1021 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
     1081 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
     1141 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
     1201 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
     1261 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
     1321 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
     1381 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
     1441 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
     1501 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
     1561 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
     1621 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
     1681 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
     1741 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
     1801 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
     1861 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
     1921 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
     1981 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
     2041 ctgtaatcat tggtttattg a
//
LOCUS       DAZBWR010000212         2029 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000212 DAZBWR010000000
VERSION     DAZBWR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.220"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..557
                     /gene="rpsN"
                     /locus_tag="ACHGT5_002859"
     CDS             288..557
                     /gene="rpsN"
                     /locus_tag="ACHGT5_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN7218785.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            572..751
                     /gene="rpmF"
                     /locus_tag="ACHGT5_002860"
     CDS             572..751
                     /gene="rpmF"
                     /locus_tag="ACHGT5_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN7218786.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            783..1160
                     /locus_tag="ACHGT5_002861"
     CDS             783..1160
                     /locus_tag="ACHGT5_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218787.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1181..1330
                     /gene="rpmG"
                     /locus_tag="ACHGT5_002862"
     CDS             1181..1330
                     /gene="rpmG"
                     /locus_tag="ACHGT5_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN7218788.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1354..>2029
                     /locus_tag="ACHGT5_002863"
     CDS             1354..>2029
                     /locus_tag="ACHGT5_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN7218789.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYH"
BASE COUNT          743 a          301 c          399 g          586 t
ORIGIN      
        1 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
       61 aaagtgaatt cttatttttc agagcttaaa ctgcatattc ttttaaatga tttgattatc
      121 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      181 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      241 ttacgatttg agatgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      301 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtacgca gataaacgtc
      361 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      421 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      481 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      541 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      601 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      661 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      721 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
      781 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
      841 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
      901 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
      961 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1021 acgaggagtt atttcaatgt attgatggtt attgggagat acctgttgat atgaaagaaa
     1081 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1141 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1201 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1261 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa ttttcaaaga
     1321 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1381 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1441 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1501 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1561 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1621 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1681 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1741 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     1801 gtcatagcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     1861 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     1921 agtgaaacga ttcaaaaaaa ggatttttat aatttggaaa aattcaatca tcccttagtt
     1981 tttgaaagtg attcatcaga aaatcactat aatggcaaac actatcatc
//
LOCUS       DAZBWR010000213         1996 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000213 DAZBWR010000000
VERSION     DAZBWR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.221"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHGT5_002864"
     CDS             complement(<1..426)
                     /locus_tag="ACHGT5_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN7218790.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHGT5_002865"
     CDS             complement(535..1566)
                     /locus_tag="ACHGT5_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7218791.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          616 a          328 c          346 g          706 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaat
//
LOCUS       DAZBWR010000214         1979 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000214 DAZBWR010000000
VERSION     DAZBWR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.222"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACHGT5_002866"
     CDS             <1..367
                     /locus_tag="ACHGT5_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN7218792.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACHGT5_002867"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACHGT5_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1979
                     /locus_tag="ACHGT5_002868"
     CDS             1330..>1979
                     /locus_tag="ACHGT5_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN7218793.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DAZBWR010000215         1966 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000215 DAZBWR010000000
VERSION     DAZBWR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.223"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..132
                     /locus_tag="ACHGT5_002869"
     CDS             <1..132
                     /locus_tag="ACHGT5_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN7218794.1"
                     /translation="NLEHQDQVTLFLDFPFLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            129..>1966
                     /locus_tag="ACHGT5_002870"
     CDS             129..>1966
                     /locus_tag="ACHGT5_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN7218795.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          731 a          242 c          390 g          603 t
ORIGIN      
        1 aatttagaac atcaggatca ggtcacctta tttttagatt ttccattttt atatcctgga
       61 aaaaatgaaa ttaaatggac gaaaaatatt cattcatttg aaataatgcc taggtggtgg
      121 agaaaagtat gaaaccaaga atatatagtc ctactgaaac agatttctca acgaatggct
      181 taggaatttt aaaggattgt acaagatgtg gaatatatga agtagctaat ggaaaatatg
      241 aattagagtt ggattatcct ttaggaacta gatttgatga atattttgaa aatgactatc
      301 aaataaaagc aaagccaaac gatcaagaag agtatcatat cttttttatt gatgataaag
      361 atatagatac ttttttaaat acagtaacta tttatgctca gagtcgtaca aatcgacttg
      421 gaagacgggt agtcactctt gtggaagtgg actctaaaac tggtcaagaa gcgatatcaa
      481 ttattgaaac taaaatggat aaaaaatctg acatacgact ttattctgat attacggctg
      541 tttctagcac aatctttgaa gcgagaaatg ttttaaattg tattgctggt gaacaaggat
      601 cattgcttca atattggggc ggagaaatta aacgtgaacc atttaagctt tctttgttaa
      661 agcgaagagg tcgcaataat gttggaacga ttcggtatgg aaaagatatg tctggtttga
      721 aggtcaaatt agattggaca ggtataaaaa caagaattat tccatatgct gatcctcaaa
      781 gtgaagtagg tacgactaat cgaatttatg gttcgccagt agatagtgcc tatattaata
      841 actatcctga tgtctataca gaacatgttc agtttacaga agaacaagga gtaaaagaca
      901 ttaagagctt aaataaaata gctaaaaatt actttaaaac gatcaatcct ggctgtgata
      961 agcctaaagt ttctattacg gttgaatttg ataagttgac tgatagtgaa gaagcgaaag
     1021 aatttgcgaa gattagaaat tatggtctgt ttgatacatt taaaatatat cataaaaaat
     1081 ataatattta tcttgaatca aaggttagtg gactacaata cgattcttta tctgaaaaaa
     1141 ctttgaaatt agaagcagga gacattcaag ttgcttttta tcagcaacaa gctgtaacta
     1201 ttcaggataa attaaaagat tatgcaacga ataattatat gagtgatttt aatgattatg
     1261 tttcttcaat gattacaggt caaggcagtg caggaggata tgtagtttta tggccaaaag
     1321 aaaaaccttc caacatcttt attatggata atccagattt agaaaaggca agagaagtac
     1381 taagaatgaa taaaaatggg attgcttttt ctaagaaagg atggcggggg ccttttaatt
     1441 cagcttggac attagatagt atatttaatg ccaattttat acaaactgga ttaatcaaag
     1501 cagatatctt tcaaaattct tttaacaaga ctggagatgt attgaaatta gttaatggac
     1561 tactccaaat ttggaacaac aaaaagaaaa taatggagtt aactaaaaaa ggaatggaat
     1621 tttggaattc taagggttca attggaacga ttggaacaac tgattctgtt ggtaatcctt
     1681 ttcctggggc ttctactcca acccctattg aagataattc tttagttatt cgtacaaatg
     1741 gagacggcaa atatattttg atttctccta aagtaggaaa aggattagtt ttattaggaa
     1801 atggtaaagc aatttatttt ggagacttag atgtacaagg aaaacttaca gttaaaggaa
     1861 aagaaataat tcctggacaa ggtggtggtc catcgggagg tggagaatct actggtgggt
     1921 atcctagtga attaaaaaca gatgcagaaa agagagcttg gagaat
//
LOCUS       DAZBWR010000216         1963 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000216 DAZBWR010000000
VERSION     DAZBWR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.224"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHGT5_002871"
     CDS             64..972
                     /locus_tag="ACHGT5_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN7218796.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACHGT5_002872"
     CDS             972..1592
                     /locus_tag="ACHGT5_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218797.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..>1963
                     /locus_tag="ACHGT5_002873"
     CDS             1596..>1963
                     /locus_tag="ACHGT5_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218798.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          697 a          302 c          443 g          521 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gac
//
LOCUS       DAZBWR010000217         1953 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000217 DAZBWR010000000
VERSION     DAZBWR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.225"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHGT5_002874"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHGT5_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN7218799.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHGT5_002875"
     CDS             853..1041
                     /locus_tag="ACHGT5_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN7218800.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHGT5_002876"
     CDS             1054..1611
                     /locus_tag="ACHGT5_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN7218801.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHGT5_002877"
     CDS             1643..1885
                     /locus_tag="ACHGT5_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN7218802.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          304 c          404 g          529 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cgg
//
LOCUS       DAZBWR010000218         1854 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000218 DAZBWR010000000
VERSION     DAZBWR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.226"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGT5_002878"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGT5_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFN7218803.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHGT5_002879"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHGT5_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN7218804.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DAZBWR010000219         1842 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000219 DAZBWR010000000
VERSION     DAZBWR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.227"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..509
                     /locus_tag="ACHGT5_002880"
     CDS             36..509
                     /locus_tag="ACHGT5_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFN7218805.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            518..745
                     /locus_tag="ACHGT5_002881"
     CDS             518..745
                     /locus_tag="ACHGT5_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218806.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1381..1752
                     /locus_tag="ACHGT5_002882"
     CDS             1381..1752
                     /locus_tag="ACHGT5_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218807.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          579 a          294 c          297 g          672 t
ORIGIN      
        1 atattttagc tatcaaagat aaaagaggga taaaaatgaa atttaaaaca ttgctattaa
       61 aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt ttttatactc
      121 agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta tttgtttctg
      181 tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg aaactattga
      241 aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta aaaaaaatcg
      301 aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa tttatgaaag
      361 tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc gtattcattc
      421 cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat gcgattttac
      481 ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg ttaatttaga
      541 tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag tgggcattac
      601 gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt tttccactct
      661 cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct ataaaaatga
      721 ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata aaaaataatc
      781 tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca gattttttat
      841 ttcgattgaa taccgtttta tctataaata agatatgtac tgactcatac aaagggggcc
      901 ttttcttctc catgtactat cacttttcct gatacaactg gtttcctgtt acgttgtagt
      961 tggatctgct tttagtataa tactttattc attccaccag actgatagca gtttgagcag
     1021 tctaaataat catgtagtat tacaggccat tctgtttcat accttgaagt gcttaaaact
     1081 tcagctaatg gcccattgtg ttaactctta ctccattctt ttttagtaag ctcccatata
     1141 tactgtaaag ccttctatat attatgtcat gttgcttttt aacatttact atgtagtttt
     1201 gataaaatga tttgaataac agtgatggac aattcttttt tatttttaac attattttga
     1261 taataatgat tatttattta aaaatacatt agataacatt tgttacgaca cgaaaaagct
     1321 tcacaaacaa atatcgttat tttaaaatga gggcgaactc acacaattag gaggaatagt
     1381 atgccattta ttgtttactt ttttctttca ttactcgtga tctatattcc attaccaaca
     1441 tttttcctgc tcgtcaatcg tctagcagag caacatgata cgattgctgc tcttacgaaa
     1501 ctttgtttac ttctatttgt ttgtctagat tactttacga gctattattg gctaaaacag
     1561 acaagagtaa aaaagagata ttatttcttc cttgtctttt tcaatctctt tgagtttctg
     1621 atccattgct ttttacttca tcagtatcat acgatccatt tgtggatcat ctgggttttc
     1681 aaagtggtcg tattattttg tatgttcacc tttcctatct caagaaaatt tagggaggca
     1741 ttgttttatt aaagaagctt ctctgtatca ataaagacac atttaaggaa aaacttttaa
     1801 aaatctaatt gggaatttgc ccgcataaat aagaggctgt ga
//
LOCUS       DAZBWR010000220         1785 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000220 DAZBWR010000000
VERSION     DAZBWR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.228"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..649
                     /locus_tag="ACHGT5_002883"
     CDS             83..649
                     /locus_tag="ACHGT5_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7218808.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            794..881
                     /locus_tag="ACHGT5_002884"
     tRNA            794..881
                     /locus_tag="ACHGT5_002884"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:826..828,aa:Ser,seq:cga)
     gene            1215..>1785
                     /locus_tag="ACHGT5_002885"
     CDS             1215..>1785
                     /locus_tag="ACHGT5_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFN7218809.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          615 a          274 c          350 g          546 t
ORIGIN      
        1 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
       61 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
      121 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
      181 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
      241 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
      301 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
      361 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
      421 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
      481 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
      541 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
      601 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
      661 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
      721 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
      781 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
      841 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
      901 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
      961 ttttttcgtt tgattgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     1021 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     1081 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     1141 ggcctttttt tgataaaaaa aatactagat gtaagataaa agtgtaaaaa cgatttgttt
     1201 taggagggga aacgatgtgt accgcaatca cttatgcaac gaaagaccat tattttggaa
     1261 ggaattttga ttatgagatg tcttataatg aagtggtcac tattactcca agaaattatc
     1321 gtttcgattt tagaaaggta aaaaatttag ataaacatta tgcaatgata ggaatagcag
     1381 ctggggtatc caactatcca ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg
     1441 caggactgaa ttttcctggc aatgctgatt ataaagaatt acaagaagga aaagataacg
     1501 ttgcaccatt tgagtttatt ccatggatac tagggcaatg ctcaaccata gacgaagcta
     1561 aagaattatt ggctactatc aatctagtca atatcgattt tagtgaaaaa ctacccttgt
     1621 cacctttaca ctggttatta gctgataaag aaaaatcgat agtaattgaa agtatgaaag
     1681 atggacttca tttatatgat aatcctgtcg gtgtgttgac aaataatccg ccatttgatt
     1741 atcaattatt taatctgaac aattatcgct cgctatcaaa tggaa
//
LOCUS       DAZBWR010000221         1720 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000221 DAZBWR010000000
VERSION     DAZBWR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.229"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1551
                     /locus_tag="ACHGT5_002886"
     CDS             <1..1551
                     /locus_tag="ACHGT5_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN7218810.1"
                     /translation="MESVKKESYLDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGR
                     PTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAIT
                     YSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQF
                     GKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTF
                     RKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHT
                     PLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGS
                     LMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAK
                     LNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKE
                     LHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDEL
                     IPLENELR"
BASE COUNT          620 a          275 c          349 g          476 t
ORIGIN      
        1 atggagtctg tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat
       61 atccaaaaag tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt
      121 cgaaatggcc gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa
      181 aagtattatc gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt
      241 cccaatcagc ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct
      301 attacctatt cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa
      361 aaaattattg aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag
      421 cgttataacg gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt
      481 gggaaacaaa tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct
      541 acgattcaaa ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt
      601 tcacgtacga accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag
      661 ccagcgacaa tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa
      721 gaatcgggtt tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat
      781 ggtaagttaa aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc
      841 cgtatcgagt acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact
      901 tatgatgatt acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt
      961 ttggaagaac aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca
     1021 gaaaaagagg gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg
     1081 tatgatattg attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca
     1141 gatgcacgag aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa
     1201 gaagtttacc gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat
     1261 atctttgtca ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt
     1321 tccaatgagt ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca
     1381 ggtgaagatc caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat
     1441 aaagccaaac atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg
     1501 cgtaaaatgt tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga
     1561 gttaagaaaa ttgtacgatt caaagatagt agtaaagtgg aagtgtagaa aatagggata
     1621 gatgtctttt tacttgttca cattttataa cggagtattt aagaaagcta ttgaataatg
     1681 attcttccat ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAZBWR010000222         1684 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000222 DAZBWR010000000
VERSION     DAZBWR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.230"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHGT5_002887"
     CDS             complement(<1..182)
                     /locus_tag="ACHGT5_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN7218811.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHGT5_002888"
     CDS             517..951
                     /locus_tag="ACHGT5_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN7218812.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHGT5_002889"
     CDS             974..1294
                     /locus_tag="ACHGT5_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218813.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHGT5_002890"
     CDS             1435..1626
                     /locus_tag="ACHGT5_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218814.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          637 a          253 c          356 g          438 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaa
//
LOCUS       DAZBWR010000223         1672 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000223 DAZBWR010000000
VERSION     DAZBWR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.231"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..652
                     /locus_tag="ACHGT5_002891"
     CDS             <1..652
                     /locus_tag="ACHGT5_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7218815.1"
                     /translation="AINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGCRV
                     SEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEKLQ
                     QLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKPFH
                     THLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES"
     gene            complement(833..1018)
                     /locus_tag="ACHGT5_002892"
     CDS             complement(833..1018)
                     /locus_tag="ACHGT5_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218816.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            1055..1348
                     /locus_tag="ACHGT5_002893"
     CDS             1055..1348
                     /locus_tag="ACHGT5_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN7218817.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1436..1582)
                     /locus_tag="ACHGT5_002894"
                     /pseudo
     CDS             complement(1436..1582)
                     /locus_tag="ACHGT5_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          594 a          253 c          314 g          511 t
ORIGIN      
        1 agccataaat aatattgagg acaagtttct tactgtcgat gaattgagaa cttttttgaa
       61 ttatggcatt gttcatgagg aattaccgat gtctgtactt ttccatgttc tattttacac
      121 tggctgccga gtcagcgagg cgcttgctct acagcctgga gatatagatt tcaaaagaaa
      181 tgaaattctg ttctataagc aaacggcagt taaaggtaag agtaaggatt ttcgaattga
      241 aactactaag acagttagtt ctgctagacg agttctagta actcctctag ttatggaaaa
      301 gctacaacag ttgattaagg ctttgggtga gatgaggcaa cattacaggt ttattataga
      361 agaaacatat ttgtttgtgt atcttgattc tagtaaacgt ggtgttcctt atcgtcgaga
      421 gtatgtaaat gatcacgtca aacggtgcgt gaatcgctca ggaattaata agccatttca
      481 tactcattta gctcgtcata caatggcaag cttagtagcg gaacactgta gctgggaagt
      541 cttgaaagat cgcttaggcc atactgacca aacaacttcg aaaatttatc gccacttaac
      601 gtcaaatgaa aaggtaaaac ctctgattgc ttttagttct ttagagagtt aaaaaaacgg
      661 tgaaagcact ggtattacag gattcttgaa aaacgatttt ttaacaaatt agtcaatatg
      721 tagtcagagg taataaaaat ccttttatat caatacttta acgacttttc acaagtatca
      781 aagaaatccg tcgttaatca ttgttatcag actgagaaag aacccttggt attcaagggt
      841 tcttttttct tagcatgtaa tttgaacacc ctgatccagt tttttcttta attattggta
      901 ggggagaata atggattaga ataaatctac ctataagtag tagtaaaaaa aagagcacta
      961 tgataatatc tttcttaaga gtaaccgctt taataaaaaa tccagaatat tctaacataa
     1021 atggagtatt cttagaatca aaaggagaaa gaagatggtt tatacttata cggagcaaga
     1081 aaagatgaaa gttttagaca cattcatgaa tgatggaaaa gtaaaacaag taccaagttc
     1141 aacaaaaaag aaatacatcc tattgaaaga agtcttgaaa cgctttgacc atggagtgac
     1201 ttatacagaa acagaggtta attcgatact gctgaacgta ttttctagtg gcgattatgt
     1261 cgaacaaaga cgttatctaa tcacatttgg ctttttcaaa agaagttcag acggtcgggc
     1321 atatcagatg atgggaattg aaaattagaa gttttaattt atactcattg aaaaaagttc
     1381 aagtatgcga tattggttct acaccgcata cttgaacttt ttttgtcatc tgtatctata
     1441 atacaaagcc aagaacgtta ttcaaaatat ggatagctat gataaaagct aatgagcgat
     1501 tcgcagttaa ataagcaatc gtcagtaaaa tcgttccagg agcgtacatg aaaaaatcaa
     1561 ttggatgcgt tacatgaata aagataattc tcttttattt gtacgaataa ataccaaaac
     1621 gtacctccta aaattagatt ttggacagat gccctttaaa aagataacaa ga
//
LOCUS       DAZBWR010000224         1666 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000224 DAZBWR010000000
VERSION     DAZBWR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.232"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACHGT5_002895"
     CDS             complement(137..427)
                     /locus_tag="ACHGT5_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218818.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACHGT5_002896"
     CDS             complement(545..805)
                     /locus_tag="ACHGT5_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218819.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACHGT5_002897"
     CDS             complement(942..1355)
                     /locus_tag="ACHGT5_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN7218820.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          633 a          267 c          245 g          521 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtaga
//
LOCUS       DAZBWR010000225         1664 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000225 DAZBWR010000000
VERSION     DAZBWR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.233"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHGT5_002898"
     CDS             131..505
                     /locus_tag="ACHGT5_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN7218821.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHGT5_002899"
     CDS             complement(619..1248)
                     /locus_tag="ACHGT5_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN7218822.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHGT5_002900"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHGT5_002900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZBWR010000226         1647 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000226 DAZBWR010000000
VERSION     DAZBWR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.234"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHGT5_002901"
     tRNA            complement(20..91)
                     /locus_tag="ACHGT5_002901"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHGT5_002902"
     tRNA            complement(113..200)
                     /locus_tag="ACHGT5_002902"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHGT5_002903"
     tRNA            complement(225..298)
                     /locus_tag="ACHGT5_002903"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHGT5_002904"
     tRNA            complement(312..382)
                     /locus_tag="ACHGT5_002904"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHGT5_002905"
     tRNA            complement(384..459)
                     /locus_tag="ACHGT5_002905"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHGT5_002906"
     tRNA            complement(478..551)
                     /locus_tag="ACHGT5_002906"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHGT5_002907"
     tRNA            complement(564..653)
                     /locus_tag="ACHGT5_002907"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHGT5_002908"
     tRNA            complement(677..750)
                     /locus_tag="ACHGT5_002908"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHGT5_002909"
     tRNA            complement(764..837)
                     /locus_tag="ACHGT5_002909"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHGT5_002910"
     tRNA            complement(865..937)
                     /locus_tag="ACHGT5_002910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHGT5_002911"
     tRNA            complement(957..1030)
                     /locus_tag="ACHGT5_002911"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACHGT5_002912"
     tRNA            complement(1040..1113)
                     /locus_tag="ACHGT5_002912"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACHGT5_002913"
     tRNA            complement(1124..1209)
                     /locus_tag="ACHGT5_002913"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACHGT5_002914"
     tRNA            complement(1226..1297)
                     /locus_tag="ACHGT5_002914"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACHGT5_002915"
     tRNA            complement(1309..1381)
                     /locus_tag="ACHGT5_002915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACHGT5_002916"
     tRNA            complement(1399..1480)
                     /locus_tag="ACHGT5_002916"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACHGT5_002917"
     tRNA            complement(1488..1560)
                     /locus_tag="ACHGT5_002917"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACHGT5_002918"
     tRNA            complement(1564..1636)
                     /locus_tag="ACHGT5_002918"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZBWR010000227         1639 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000227 DAZBWR010000000
VERSION     DAZBWR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.235"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            415..>1639
                     /gene="ltrA"
                     /locus_tag="ACHGT5_002919"
     CDS             415..>1639
                     /gene="ltrA"
                     /locus_tag="ACHGT5_002919"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN7218823.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          585 a          299 c          388 g          367 t
ORIGIN      
        1 acttggtggt gaaagtccac tacaggcttg gcagtaggaa ctgttagcga aagacaaggc
       61 ggcaatcgtg aggttgtggc tgaaggaagt cggacgcaaa ctcctgaact gacgaacaga
      121 aactagatac aaggctgaat taggtcggat aagtcttcat cacaagacaa agtccaatac
      181 tgcccgaaac ctatacagta aatctagcag ttacatggga ggaaagtttt cactcttacc
      241 gggggagatc tcatgagggt gttgtgggcg ttgaatgaaa acaaaccagc acaacgatca
      301 tggcgacatg atggtgaatc atgagaagtc agccgaggtc atagtagtaa cgacactgac
      361 cgttacgaag gactgaacaa taagaatctt gaataattta ggaggtgtga gaagatgcga
      421 cgaccgcaga aaacaagaaa acttggctat catcaaagag ctagtgtgga acacgaaggg
      481 tatgatgaag tgcgcagcac ttcttgcggt gaacagagga aacaagatgg tgtagaaaat
      541 ctgtttggga gaattctaga tcggaataat ctcaatcaag cgtatcttca agtagtcaga
      601 aataaagggg cggctggtgc agatgggatg accgtcgatc aactgcttcc ttacttgaaa
      661 gagcaccgcg aggaattact atccaattta cgccaaggga actataaacc tcagcctgtt
      721 ttgcgagtag aaataccgaa accaaacggc ggtgtaagaa aactaggaat tcccacggtt
      781 attgatcgta tgcttcaaca ggcaatcaat caagtgttac aaccgctatt tgaaccagaa
      841 ttctcagata atagttatgg gtttcgccca aatcgcagcg cacatgacgc agtgaagaaa
      901 gcaaaatctt attatgaaca aggttaccga tatgtggtgg acattgatat gaaagcctac
      961 tttgacaccg taaatcacga taagctgatg tatttcattg agcggaaagt aaaggacaaa
     1021 caagtcctaa aactgatccg tcaatactta atgagtggtg taatggaaaa tggactcatc
     1081 acaacaactg aagaaggaac gccccaagga ggcaatctct ctcccctgtt aagtaacatc
     1141 tacctgaacg aatttgatca agtgctagaa aatctaggac ataaatttgt tcgatacgcc
     1201 gatgactgca atatctatgt tagaagcaaa cgagcaggac agcgtgtgat gaagaaatcc
     1261 attcaatttc tcgaagaaca actaaagttg acggtaaatc gagaaaagag tgcggtagga
     1321 agtccattga aacgaaagtt tcttggattc tgcattctgc caacaaagaa aggtattaaa
     1381 attcgccctc acgcaaaagc aaaacagcgt gtgaaaagca agctcaaaca actcactaaa
     1441 agaaaccgtg gaagaagtat ccaacttatt ttaaaagaaa tcaagcaact aatgactggt
     1501 tggatcaact actatggaat tggtgagatg aaagaattca tgagggagtt aaacggttgg
     1561 ttgaagcgaa gaatcagaca atatatttgg aaacaatgga agaatccaag aaccaaaaga
     1621 aaaaatctga ttcgtttag
//
LOCUS       DAZBWR010000228         1604 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000228 DAZBWR010000000
VERSION     DAZBWR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.236"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHGT5_002920"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHGT5_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHGT5_002921"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHGT5_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          566 a          242 c          213 g          583 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaa
//
LOCUS       DAZBWR010000229         1596 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000229 DAZBWR010000000
VERSION     DAZBWR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.237"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..1425
                     /locus_tag="ACHGT5_002922"
     CDS             256..1425
                     /locus_tag="ACHGT5_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN7218824.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          583 a          225 c          259 g          529 t
ORIGIN      
        1 aaggtaataa gttattcttt tattactatg tttgggtttt atagcaaaga aagtttaata
       61 agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat ctttagtgat
      121 ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta aatagatatt
      181 tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac cgaaatttta
      241 taggaggact ttctcatgcc caattttact acagaaatta tggaaacact aatcaataaa
      301 ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc attattgcag
      361 gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt caattcaggt
      421 aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga attaaatttg
      481 gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc ttataaaaga
      541 accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat tactatgtct
      601 gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca aaccatttca
      661 aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc cttagaatcc
      721 agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca aactgtttca
      781 aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga ggttcttggg
      841 tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca agacctaaaa
      901 gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg cattaacgat
      961 agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca tatatctaga
     1021 aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga tttcaaatta
     1081 gtttatcaag caagttcaaa agaagaagca aataatcaaa taaggtttat gatagataaa
     1141 tggaaaaagc agtatccacg agtggttaaa ttgctcatga atcctgctat attaaccttt
     1201 tataatttcc ctccgtcaat cagaagaact atttattcaa ctaatttgat tgaaggtttt
     1261 aacaaacaat taaagaaata taccaaaaga aaagagcaat ttcctaatga agaatctctg
     1321 gagagatttc tagtatctca attcaacaac tataatcaaa agtttctatg ccgagttcat
     1381 aaaggtttta aagaaataca tgatacatta gaatcaatgt tttaacttga tcaacagaat
     1441 gggcttttcc atttacacaa aattattgac gctcccgaca aaattatgat cttgcgtaaa
     1501 gttataaaat attaataaaa atattttatt aatgaaaagc ttggtagcac ggttattcat
     1561 atgctaattt atttcacttt atagtaaggg gagttc
//
LOCUS       DAZBWR010000230         1589 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000230 DAZBWR010000000
VERSION     DAZBWR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.238"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1542
                     /locus_tag="ACHGT5_002923"
     CDS             76..1542
                     /locus_tag="ACHGT5_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN7218825.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          441 a          291 c          333 g          524 t
ORIGIN      
        1 ataagttttt tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga
       61 attggggtat agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga
      121 ttgtcaactt tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca
      181 ttacttatct attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca
      241 acagcattag ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc
      301 ggatgggtat ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc
      361 attatgcttg gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg
      421 atggcattga ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga
      481 catctttatt caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt
      541 gtcaacttag gggcattatt agcaccacta atcgttggta caatcggtca agaatataac
      601 tatcatttag ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc
      661 atccaaggac gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact
      721 gaagaacgta aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta
      781 ttcggcgggg cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt
      841 gtattgggga ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta
      901 actgctgaag aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt
      961 ttctggtcat tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca
     1021 caatctacgc tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt
     1081 ttcatcttta ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa
     1141 ccatctacag tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta
     1201 ttgatgttgc cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg
     1261 ggaagtttct tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg
     1321 acaacaaaat tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct
     1381 gatgcttctt cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa
     1441 tctgcttact tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact
     1501 gtgaaagaac caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca
     1561 ggaattgctt atttgcagca gttcctgtt
//
LOCUS       DAZBWR010000231         1514 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000231 DAZBWR010000000
VERSION     DAZBWR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.239"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..>1514
                     /locus_tag="ACHGT5_002924"
     CDS             153..>1514
                     /locus_tag="ACHGT5_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218826.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLE"
BASE COUNT          548 a          207 c          275 g          484 t
ORIGIN      
        1 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
       61 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      121 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      181 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      241 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      301 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      361 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      421 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      481 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      541 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      601 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      661 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      721 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      781 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      841 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
      901 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
      961 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1021 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1081 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1141 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1201 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1261 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1321 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1381 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1441 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1501 attgtttact tgaa
//
LOCUS       DAZBWR010000232         1509 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000232 DAZBWR010000000
VERSION     DAZBWR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.240"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1046
                     /locus_tag="ACHGT5_002925"
     CDS             <1..1046
                     /locus_tag="ACHGT5_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN7218827.1"
                     /translation="SVKISVIVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTDN
                     TLAILKKYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFESL
                     YNCAIAENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNRL
                     YSHNAWDKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQ
                     QNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKN
                     VGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMY
                     KTAYNMLWRIYNR"
     gene            1060..>1509
                     /locus_tag="ACHGT5_002926"
     CDS             1060..>1509
                     /locus_tag="ACHGT5_002926"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN7218828.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKY"
BASE COUNT          594 a          149 c          283 g          483 t
ORIGIN      
        1 tgtcagttaa gattagtgta attgtacctt gttataatgt tgaaaaatat attgatgagt
       61 gtataaatag tttgataaat caaacattga aagatattga gattattttg ttaaacgatg
      121 gttcaacaga taatacgttg gcgatattaa agaaatatga aaagcttgat tctagaataa
      181 aagtttacga acataaaaat atggggttag ggccaactag aaatcgaggg atcaaaatag
      241 ctaatggaga atttttggct tttgtggatt ctgatgattt tgtttccaat actatgtttg
      301 aaagtttata taattgtgca attgcagaga atgcggacat tgcaattgga gaagtaggca
      361 ttttttctga tagagaaata gaaagagtaa aaataagaaa aagtctaacg aacttaaaaa
      421 caattgaatt agcaacatat aacaagaaag aattttttaa gcaattctat ttcaatcgtt
      481 tatattcaca taatgcttgg gataagatat atagacgtag tttagtatta gagtttaatt
      541 tatcatttgg tgataacaaa ataatttttg cagaagataa ttggtttcaa ttacaagtat
      601 ttatgacaaa tacaagaatc tgtttcgtag ataaattgtg ttattattat cgccaacaaa
      661 ataattctat aatgcataag ccaaaaagta atttggtttt tagacatgga aatatgattg
      721 ctgattatga aaagttgata aggcaaaatg gaaacaacat aatagataaa aaagcttgta
      781 tgttggttgc atttgatgta ctaattatgg agatttttaa tcaaaaaaac gttggtggaa
      841 catgtgaaga ttataagaat gcattagttt ctttgaaaca tcataaaata ttgagagaaa
      901 atatttcaaa tatagttaga atgaaagcat atgaacttga acctaatatg agaaagaagt
      961 tagcccttgt gaccattagt ataatgttta attttaaaat gtataaaaca gcgtataata
     1021 tgctctggag aatatataat cgataaagag gtaggaaata tgaggaaatt gacctttgtg
     1081 ataggaatta tgggaaaagg aggggcagaa cgagtaattg cgaatttggc aaataatttt
     1141 attcaagata actgggaaat tgatattata actatatatg ggaatcggca agactatgaa
     1201 ctgaatagaa aaatacaggt acatccaata atatgtaaaa gttcgtttcg tgcatttaga
     1261 cctttggaac gctttaagga acttaggaaa aaaataaggg aaatacagcc tgatctggtt
     1321 atttcgttcc ttgctgatgt aaatatccat gtcattattt cattgttagg agtaaaacaa
     1381 aaattaattg tttctgaacg aaatgatcca caacggaatc cagaagaacc atggattagg
     1441 aaaattagag attttcttta taaaaatgta gatggaattg tttttcaaac accagacgca
     1501 aaaaaatat
//
LOCUS       DAZBWR010000233         1501 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000233 DAZBWR010000000
VERSION     DAZBWR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.241"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACHGT5_002927"
     CDS             complement(<1..125)
                     /locus_tag="ACHGT5_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFN7218829.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ACHGT5_002928"
     CDS             complement(143..463)
                     /locus_tag="ACHGT5_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7218830.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..>1501
                     /locus_tag="ACHGT5_002929"
     CDS             762..>1501
                     /locus_tag="ACHGT5_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN7218831.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          557 a          258 c          234 g          452 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
     1021 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
     1081 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
     1141 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
     1201 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
     1261 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
     1321 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
     1381 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
     1441 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
     1501 g
//
LOCUS       DAZBWR010000234         1497 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000234 DAZBWR010000000
VERSION     DAZBWR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.242"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1231
                     /locus_tag="ACHGT5_002930"
     CDS             62..1231
                     /locus_tag="ACHGT5_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN7218832.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          530 a          249 c          246 g          472 t
ORIGIN      
        1 aaaaaagtcc attctgtaaa attaaagtta ccacacagaa attttatagg aggactttct
       61 catgactaaa tttactatag aaattatgca aacactactc aataaagggg atttagatga
      121 attatttcag gatcatttag agcgagcgat taattcactt ctacaagcag agctaactgc
      181 ttttttagat tacgaaaaat atgatcgaaa tggattcaat tcaggaaatt cccgtaacgg
      241 gaattattca cgtacattta aaacagaata tggcgagttg aatttaacta ttcctagaga
      301 tcggaatggc gaattttctc aacaaacact accagcatat aagcgaacca atgattcatt
      361 agaaacgacc attatccaat tatttcaaaa aggcattact atggctgaaa tctccaaatt
      421 aattgaaaaa atgtatggtc accactatac gccacaaaca atctccaaca tgagtaaatt
      481 ggtggctgaa gatgttttag cttttaaaga aagaacgtta gaagccaatt attccgttat
      541 atttatggat gcgacgcata tccctgtgaa gcgacagact gtttcgaagg aagcagtgta
      601 tattacgatt ggtattcgtt tggatggaac caaagaagtt cttgggttta ctattgcccc
      661 aactgagtct gcttatattt ggaaagaagt tcttcaagat cttagaaaac gtgggttaga
      721 agaagtttta ttagtagtga cagacggatt aagctgtatt gaagaaagta tccatagtgt
      781 gtatccgaat gcccaatttc aacaatgttg tgtgcatgta tctagaaata tcgctcataa
      841 agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc aaattggttt accaagcgaa
      901 ttcaaaagaa gaggcattgg atcacatcga ctttatgact aggaaatgga aaaagcagta
      961 tccaagagtc gtcaatttac tcttgaatcc tgccctatta accttttata atttccctca
     1021 cgccatcaga cgaacaattt attcgacgaa cctgattgaa ggctttaata agcagctaaa
     1081 acgatatact cgaagaaaag aacaattccc taatgaagaa tctctagaga gattcttcat
     1141 ttctcaattt aatcaatata accaaaaatt tttaggtaga attcacaagg gatttaaaga
     1201 aattcaggat acattagagt cgatgattta actgtaatta acagaatgga ttttccattt
     1261 acacataatt cttgacgcta ccataatcta aataaccatc atattcatct actacttata
     1321 taattgagtt ttattaaact ttttaagttt ctattctatc aaatatttaa acaatctctg
     1381 catccgaaaa gactacctcg tatagtttta ctaacaatat ttttattagc gtgttcaaat
     1441 cctgatttct tgtatcaatt attttctgtt cactttcaat aagaaaaaag tctttac
//
LOCUS       DAZBWR010000235         1480 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000235 DAZBWR010000000
VERSION     DAZBWR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.243"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHGT5_002931"
     CDS             64..972
                     /locus_tag="ACHGT5_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN7218833.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHGT5_002932"
     CDS             972..>1480
                     /locus_tag="ACHGT5_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218834.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAZBWR010000236         1435 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000236 DAZBWR010000000
VERSION     DAZBWR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.244"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(211..284)
                     /locus_tag="ACHGT5_002933"
     tRNA            complement(211..284)
                     /locus_tag="ACHGT5_002933"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Ile,seq:cat)
     gene            complement(473..>1435)
                     /locus_tag="ACHGT5_002934"
     CDS             complement(473..>1435)
                     /locus_tag="ACHGT5_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN7218835.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          425 a          287 c          256 g          467 t
ORIGIN      
        1 tcacaatata acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt tcaaatatac
       61 agaaatataa ctatgtgaaa catcctttca ttaatccata aaaggataca taaaaaagcc
      121 actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaattct
      181 aacacagaac gattcaaatg gctacgttaa tgtaccctgt aggactcgaa cctacgaccg
      241 gacggttatg agccgtctgc tctgaccaac tgagctaagg gtactggttg ttgccacata
      301 aagccataaa caatcaacca gtagaatgtg tggcaacaaa cctgttatcg catagcttgg
      361 agtgtgacta ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaatga
      421 tttttatagt tgtcaatata gttatgtaat gctcctcaac gaggagctat ttttatcgtt
      481 taggaatatt taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc
      541 cctctggatc attcgttgcc cgcatcatga catagacttt cttattaggg aagttacgca
      601 tgttaaaaga tacatgatag ccaacatttc cagaagtatt ataagcttga tttacatctg
      661 ctctataaat tccatcagct cttactcgag ctaattcttt cccagtattg taatccataa
      721 tgaagatata ctcgtattta tagttagcaa tgtgccatcc agctacatgc aaatttgcat
      781 tttcgatttc tccaaactga tcaatgtggg cgtaattcgt tccatctgtc agtgtaggat
      841 tagctgcacc agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt
      901 catcggtaaa tccatgagct aaatcatatg ctaatttttc tttacttacg cccatttcag
      961 aaagataacc gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc cataaatgcg
     1021 atttgattcc cggttggtta taaggagtgt ccaacgttaa tggaatacca tatttcattg
     1081 ctgaatctct agccaattca acgtatgcct tgtagttttt ctcaaacgtt gctttatcat
     1141 gtgtgtgttg taactcaatc tgcacaggac tgttggcatt agcatacgaa ccagcaccgt
     1201 actgtacata accaggttga ccgacttgat aaacaattcc gccgtctccc acaatataag
     1261 cagtataagc gctagtccat gaacgttgca tatactgcgc ttcattgcgt cctgttgctg
     1321 tttcattagc cgtttcatgc agtaaaatat actgattatt tgctacttgt gagctacctt
     1381 catttgcgcc caaattaaat tcattgttga tagtataggc aaatccgtta attgg
//
LOCUS       DAZBWR010000237         1383 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000237 DAZBWR010000000
VERSION     DAZBWR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.245"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            376..606
                     /locus_tag="ACHGT5_002935"
     CDS             376..606
                     /locus_tag="ACHGT5_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218836.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            612..911
                     /locus_tag="ACHGT5_002936"
     CDS             612..911
                     /locus_tag="ACHGT5_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN7218837.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          487 a          213 c          221 g          462 t
ORIGIN      
        1 cagcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
       61 aatttccgtg aatttcttct tatttcttgg agttaaacgc ttctgttcca gcctcttttt
      121 atctgtcttt cactaatgat cagttgtaga taagcaagtt gctttttctt acatcatacc
      181 gcccatcaag acaaacgttt ttagcttgta cgtactggac aaaaatccca ttatatcaag
      241 aaaaaacaac gttgataata cttaaaatta ttaggctaga acggactagt aagagttttt
      301 tgcccctttt ttgcccctca aatatcgctt tacagccaga acataagttc gtataatcgt
      361 tttgaggtga tttttatgat ggaagacttt attagaaaaa atatcagtga tgaatatgca
      421 gacttttatg agcaaagcag caaaaaagat aaattccaga tggatgtttc aattttagct
      481 atattagctt tttccgaaaa taaacaacct gtaactgcaa aaaaagaaac agtattctct
      541 gaaggcaaaa taaaaactcg atatatatta gaggtagaga ctaagtttaa aaatagatcg
      601 gagtaatggt tatgcttttt aatgagacgc aattatggtt taaatttgat ccttcgaata
      661 gatttgtcaa agatttttat aaggtgtggg attcagaagt tttcttttta gcaatcgaag
      721 atagcttatt aattaatctc tactattcta ataagaacta ctttaaaatc cctgctgcga
      781 aaactagaat gaagaaagac gtatattttt tgtttgatat cgtgactgat gtgctagacg
      841 ctcgaagcga tcatcggcgt tatgactata taaagtatac tttcgttgat ccagaaagat
      901 acaaagatta aagtaggcta cctaaaaagg tagcccggaa cggattttat caccatactt
      961 atgaaaggag atatttttta agttagtatt aagattgtgt aatatgatga tatctatatt
     1021 ttatagtatc agtgctataa aatcaaaaat aagtcactaa ttaactacca ctccaattat
     1081 aagccttttt tctcactttt tttcaaaaat atggtatgct ttttaatggc ttcaaatata
     1141 aaagagttta aagcgtaaca cacttatggg gaagtggttt ggggtgcgct ttaaactctt
     1201 ctttattatt atctcacaat ttaacccaaa tgtctttcta tttaaaaatc aaagtaaaac
     1261 ttttcaaata tacagaagta taactatgtg aaacatcctt tcattaatcc ataaaaggat
     1321 acataaaaaa gccactcatt tgagtggcaa tgaagaaaag ctttagcttg tataatactc
     1381 ttc
//
LOCUS       DAZBWR010000238         1348 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000238 DAZBWR010000000
VERSION     DAZBWR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.246"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..>1348
                     /locus_tag="ACHGT5_002937"
     CDS             join(33..474,474..>1348)
                     /locus_tag="ACHGT5_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN7218838.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVE"
BASE COUNT          566 a          173 c          235 g          374 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaa
//
LOCUS       DAZBWR010000239         1334 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000239 DAZBWR010000000
VERSION     DAZBWR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.247"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..771
                     /locus_tag="ACHGT5_002938"
     CDS             217..771
                     /locus_tag="ACHGT5_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7218839.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            827..>1334
                     /locus_tag="ACHGT5_002939"
     CDS             827..>1334
                     /locus_tag="ACHGT5_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218840.1"
                     /translation="MKITEFSEVFKTFSNIYNRIPEIEQNVVVDNDYYIENDFNKPRK
                     YDNLYNFIHNRTTINNNSNTRVLINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAI
                     NNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIF
                     ASIHDYIGL"
BASE COUNT          545 a          136 c          234 g          419 t
ORIGIN      
        1 taaaatttaa ataagagctt agaatcttca tataagtaag gttaattttt ttgtacaaaa
       61 aagtcggaat attctgagtt actgaaatga tataaaatca taaactaaac cccttgattt
      121 tcttcacttt taacagagta tatatataag ttcatgatta tggtaaaata tatatataat
      181 catgaactca gaatattttt aagaagggaa acaatagtga gctataatgt acaaccgtta
      241 aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag taatccagaa
      301 agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc agatattata
      361 ggattaaaag ttggagacat acgaggtaaa agtaagccta ttattgttga acataaaaca
      421 ggtaagcgaa aaccgatttt tatagataat ttgagagaag agattttatt atatactgaa
      481 ggtaaagaag aaaatgattg gttatttcca agtcggcaac aaggtcgaca tataacaagg
      541 gatcgtgtct atcagatata tgcagatata gcagaaaaat taggaagaga tgatatagga
      601 acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag agacattgct
      661 accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta cattggtata
      721 actgaagatg aaataggggc atcgttacgt ggttttaaat tgggaggcta attgagatat
      781 taggatatat tttttgtgta taatgaaaac ttagaaggtg agattaatga agataactga
      841 attttcggaa gtatttaaaa cgtttagtaa catttacaat agaataccag agatagaaca
      901 aaatgttgta gttgataatg attattatat agagaatgat tttaataaac caagaaaata
      961 tgacaaccta tacaatttta tacacaatag aacaacaata aataacaaca gtaacactcg
     1021 agttttgatt aattcagcat attttcatat taaatattta aagaaatatg agtatgaaga
     1081 agttgtatta gattatgggc taaatagaca gtcattttta atttatgcaa taaataacgg
     1141 aaatttgcca agcaataaaa agaacataat tattgaaaca tatgcctatc tggagggggg
     1201 atctaaaaaa ctaattaagt catgtaatat agagtatcaa ttatcagtag gagaaattaa
     1261 atgtgttttc gaagaagatt tagaacttga cttaaaaaaa atttttgcca gcattcatga
     1321 ttatataggt ttga
//
LOCUS       DAZBWR010000240         1310 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000240 DAZBWR010000000
VERSION     DAZBWR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.248"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..741
                     /locus_tag="ACHGT5_002940"
     CDS             199..741
                     /locus_tag="ACHGT5_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218841.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            897..>1310
                     /locus_tag="ACHGT5_002941"
     CDS             897..>1310
                     /locus_tag="ACHGT5_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN7218842.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          531 a          148 c          255 g          376 t
ORIGIN      
        1 ttgatgaagc agatcccaaa gggctgcttc atcaattttt tcgtaagtgt ccgtacactt
       61 actgtgcttg ctcatatatt aatcgatcat atactaatcg tgtaagaaca actttaagca
      121 taatacttat attgttatac catagtttgg aaaaataaaa aattatattt catcaacgtt
      181 tcataaaagg tgtgtagaat gttattgaaa gtagctataa aatgctattg tgtaaaaaat
      241 aaaggaggaa taatttatga aaaaatagta ccaagaggag catgcctatt acttgcatta
      301 tgtttgagct taagcgcagt tgggtgtcaa aaaagtaatg aaaacaataa aataaaagaa
      361 ggacaatcca taagttctga agaggaaggc atgtcaatta atgcaaatgg caaaaatgaa
      421 acttttaagc catcagatta tacattggag gcaaagaaag aatatgttta tgaatattta
      481 gggcttaaat ttaaactctc agacaaattt agaaattata tagctgataa aaagatagcg
      541 atgttagatg atcaaagtcc tatagataaa gaactaaagt atgctatttt gacatttgag
      601 aaaatgacag aagaacaaaa gaatgcagtt atagaaaaga tgggcgatgg atataaaaat
      661 tggcaaaatg agcttgaaag aattgggact attggtatat ttgaaaaaaa tacatcagaa
      721 gaaaaaaatc taaaattata aaatgtgata ctcatactaa aataggagtt tcaagtgatg
      781 gaaaatacga ttgctattta agcacaaata gcggagctga aagtaatctt ctggatgaat
      841 taaaaagaac tgagattcaa attatagata aaaaagaacg tcctaaaaat ggttttgtgc
      901 tatcagaaaa aactgattta gaaaatacag aagcatttaa caaagaatcg gttaaagatt
      961 tacgtaaact accaacaaag gacataaatg gcaaggattt tacaagtaaa gattttgaga
     1021 agtatgatct tactatggta aatgtatttg caacttggtg tacagcttgt gtaaaggaaa
     1081 ttcctgattt ggttgaggtt cagaatgaaa tgaaaagcaa gggtgtaaat atcgttggag
     1141 ttgttacaga tcctgttgat gataacggtg aaaacaagga agcaattgaa aagtcaaagt
     1201 taatacatga aaagacaaag gcttcatatc ctttcttaat gccagatgga atggcaacac
     1261 tttttttgac aaaaattata aggtgttgtg aatgctgcgg tgttatggct
//
LOCUS       DAZBWR010000241         1276 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000241 DAZBWR010000000
VERSION     DAZBWR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.249"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..532
                     /locus_tag="ACHGT5_002942"
     CDS             35..532
                     /locus_tag="ACHGT5_002942"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN7218843.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="ACHGT5_002943"
     CDS             complement(569..1252)
                     /locus_tag="ACHGT5_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFN7218844.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DAZBWR010000242         1263 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000242 DAZBWR010000000
VERSION     DAZBWR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.250"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..224
                     /locus_tag="ACHGT5_002944"
     CDS             <1..224
                     /locus_tag="ACHGT5_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN7218845.1"
                     /translation="VFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEE
                     DFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            229..1176
                     /locus_tag="ACHGT5_002945"
     CDS             229..1176
                     /locus_tag="ACHGT5_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN7218846.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          414 a          179 c          263 g          407 t
ORIGIN      
        1 aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta
       61 attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata
      121 ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt
      181 cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat
      241 cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa
      301 catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg
      361 aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg
      421 aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca
      481 gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa
      541 ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt
      601 tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct
      661 tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat
      721 gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct
      781 ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa
      841 gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat
      901 gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc
      961 aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt
     1021 ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct
     1081 ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt
     1141 ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg
     1201 gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata
     1261 gaa
//
LOCUS       DAZBWR010000243         1252 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000243 DAZBWR010000000
VERSION     DAZBWR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.251"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..722
                     /locus_tag="ACHGT5_002946"
     CDS             <1..722
                     /locus_tag="ACHGT5_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFN7218847.1"
                     /translation="FLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLP
                     VLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVG
                     YSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEI
                     FLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQY
                     IGKKVKRPISADSVLLEEDVE"
     gene            719..>1252
                     /locus_tag="ACHGT5_002947"
     CDS             719..>1252
                     /locus_tag="ACHGT5_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN7218848.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDT"
BASE COUNT          413 a          167 c          271 g          401 t
ORIGIN      
        1 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
       61 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
      121 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
      181 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
      241 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
      301 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
      361 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
      421 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
      481 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
      541 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
      601 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
      661 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
      721 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
      781 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
      841 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
      901 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
      961 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     1021 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     1081 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     1141 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     1201 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ct
//
LOCUS       DAZBWR010000244         1242 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000244 DAZBWR010000000
VERSION     DAZBWR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.252"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1002
                     /locus_tag="ACHGT5_002948"
     CDS             <1..1002
                     /locus_tag="ACHGT5_002948"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN7218849.1"
                     /translation="KGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQ
                     QELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMG
                     TQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSC
                     VRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFG
                     ETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIER
                     SYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1014..>1242
                     /locus_tag="ACHGT5_002949"
     CDS             1014..>1242
                     /locus_tag="ACHGT5_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFN7218850.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLE"
BASE COUNT          494 a          161 c          234 g          353 t
ORIGIN      
        1 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
       61 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
      121 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
      181 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
      241 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
      301 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
      361 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
      421 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
      481 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
      541 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
      601 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
      661 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata
      721 tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca
      781 ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat
      841 gataaaatgt atatcattcc accacactat catgctactg acgaactgta caaagattat
      901 gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa
      961 tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat
     1021 cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt
     1081 tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta
     1141 aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta
     1201 aagaggacag ttctcaattt gaaatgctaa aaaaattaga at
//
LOCUS       DAZBWR010000245         1195 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000245 DAZBWR010000000
VERSION     DAZBWR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.253"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHGT5_002950"
     CDS             <1..1076
                     /locus_tag="ACHGT5_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN7218851.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZBWR010000246         1149 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000246 DAZBWR010000000
VERSION     DAZBWR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.255"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..797
                     /locus_tag="ACHGT5_002951"
     CDS             <1..797
                     /locus_tag="ACHGT5_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN7218852.1"
                     /translation="MNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIRE
                     VLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEK
                     EVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTT
                     DVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGI
                     VLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            799..>1149
                     /locus_tag="ACHGT5_002952"
     CDS             799..>1149
                     /locus_tag="ACHGT5_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218853.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKL"
BASE COUNT          382 a          140 c          191 g          436 t
ORIGIN      
        1 tgatgaattt tatcgtacaa atattttata ttacactttc ttcgtctaga ggtgcccagg
       61 tagtaatttg ttctttttta gtattatttc ttttgttgtt tgccaattca aaatttagaa
      121 agagcattag agaagtacta cctgtaaaaa tcaaatggat ggtaagcata ttattagttc
      181 tattggtata tttttctttt ggtactaatt atgctaagga ttttttagca acaattcctg
      241 tatcgattga tagaaaagta gaagttatag agtcaaatga atcagataaa actccacttc
      301 cagaaaaaga agtaatatta cagcattcta gtgataactc cgaaatttca gctggtcgtt
      361 ttacgatttg gaaagctgcg cttaaagtta taagaccaac tttgttattt ggttatggag
      421 agaccgattt ctatgggaat agcgatgcaa aatttttgaa aaaagtagat cttgatacta
      481 cagatgtgaa tgaactaaaa agagcacatg gaaacatgca taatggattt cttcaagtac
      541 ttgtatcaag tggtataatt gcttttatat gtatctatgc tttctatgtg ttaaatatct
      601 ttagtttact caaaacattt tttagaagta aagtgaatta tgcgtttctt ggtattgttt
      661 taatttttat actatctatt tttatagatg aaatggtgga ggctcatgta cttttcaata
      721 agcgcgatgt catttcaatt gttttctggt attatttggg aagcatttct tacttgtatt
      781 taaaaaagga gaattaatat gaagacagct ttcattgctt ggactccttt acatttgatt
      841 aatatattaa atgtttatca aaatttttac cgagaatcaa aaggagattt atttatttat
      901 tctgaatttg aaaacgctag aagatatagt gagcgtataa aagaattagg gctatttaat
      961 gatgttgttt ttgtgaatca ccggaaaatc ggttcttcct ttaaacgtaa aattaacttg
     1021 ttaactaacc ataatcaatt tttctcggat gatttatccg tttatgatga aatttttata
     1081 cagggggaaa attatttcac aaaaatactt ttctccaatg tttacaaaaa aaatccagat
     1141 gtaaaacta
//
LOCUS       DAZBWR010000247         1140 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000247 DAZBWR010000000
VERSION     DAZBWR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.256"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..>1140)
                     /locus_tag="ACHGT5_002953"
     CDS             complement(1..>1140)
                     /locus_tag="ACHGT5_002953"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN7218854.1"
                     /translation="DRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNRANE
                     DIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARSFEV
                     KEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAMMITQN
                     PRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKELSGRG
                     LEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNLYTGSL
                     YLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEMLDSR
                     TRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          313 a          246 c          219 g          362 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
//
LOCUS       DAZBWR010000248         1113 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000248 DAZBWR010000000
VERSION     DAZBWR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.257"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..945
                     /locus_tag="ACHGT5_002954"
     CDS             <1..945
                     /locus_tag="ACHGT5_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN7218855.1"
                     /translation="INDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNVAANMKNNY
                     NGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRA
                     YIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKE
                     KLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHV
                     AGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNF
                     PSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          381 a          193 c          224 g          315 t
ORIGIN      
        1 attaatgacg agtataattt agcgccgaat caaggagact ccagattagc aattcctaac
       61 aaaattattt tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg
      121 aaaaataatt ataacggaag caattcttat actacagatg ttattggtga cggtgggatt
      181 gtttaccgtg tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat
      241 gcgcctgtac agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat
      301 cgagcttata ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt
      361 gatcaaggaa cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat
      421 gtttggggga accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa
      481 aagcttgctt atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      541 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      601 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      661 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      721 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      781 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      841 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      901 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      961 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
     1021 atcgcatatc ttcactatca cccataaata gtcacactcc aagctatgcg ataacaggtt
     1081 tgttgccaca cattctactg gttgattgtt tat
//
LOCUS       DAZBWR010000249         1113 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000249 DAZBWR010000000
VERSION     DAZBWR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.258"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..248
                     /locus_tag="ACHGT5_002955"
     CDS             <1..248
                     /locus_tag="ACHGT5_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218856.1"
                     /translation="VLNLKMFIIEKWIVIIAKIFSSISIIIPMTLTFSKEQFNVATTF
                     VSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(307..477)
                     /locus_tag="ACHGT5_002956"
     CDS             complement(307..477)
                     /locus_tag="ACHGT5_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218857.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(477..776)
                     /locus_tag="ACHGT5_002957"
     CDS             complement(477..776)
                     /locus_tag="ACHGT5_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218858.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(881..1084)
                     /locus_tag="ACHGT5_002958"
     CDS             complement(881..1084)
                     /locus_tag="ACHGT5_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218859.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
BASE COUNT          323 a          219 c          164 g          407 t
ORIGIN      
        1 tggtgcttaa tttaaaaatg tttataattg aaaaatggat agttattatc gcaaagattt
       61 tctcatcgat atcaattatc attccaatga cattgacgtt ttctaaagag caatttaacg
      121 tagctactac attcgtatct tttatagctc tattttcgtt tgctgttcct aaaacttttc
      181 aaagcaaatc tagcaacgca aaggttaatg tagaaaacac tgatagaaaa aatgatgaaa
      241 gttattaaaa aaaataattc tttcatttat tgtcaaacct ttcttataga cattagactt
      301 ttttagtcag tccctcccga ctggcttttt tgttttgtac tcagcttcat ccagccctat
      361 aaaaatccaa accatgtaca caatcgtacc tatcaacgct tgtctgcttc cccagaggcc
      421 taaagcgtag atgattagcg gtgcgctgaa tactagtgct ctgttgaatt tgcccattaa
      481 cttgcctcct tcaaaacatc tgaaggcgat aaacttttta cacgatattt gtttgcgtct
      541 ttccacttta aaaaccaaat aaaagtatgg taatgaataa atgtggttga gtgacctgga
      601 cgaagtattc cttctgcaaa ttcaggaatt gtttccattt ccttgcagta ttcaactaaa
      661 gtagtttttg acatgccgtg gaacttctcc aagattaaac tttgtcggta ccagtcgtct
      721 ggttttactg ccttctcagc agcttctatc agctctgaaa gtgttggttt tttcacttat
      781 tatccctcct aaaaattatc gttccatact cgatgtttaa catttcttga agctctgatt
      841 ttgtaagaat gattttatct tccaacgact ttacctccct ctacaattcg tacatagtga
      901 taatcgaatc tataattctg tttgcttctg cagaagtctt tttaccgttt aaaattaaag
      961 ataagtagct tttgctaatt ccaaatcttt cagcaagcat ggtgtaagtt aagaactttg
     1021 aactttcgac atattctttg attttttctc tatctcgttg agtgatttct gcaatgtcag
     1081 tcatactaaa actcctttct aaccaatttc ctc
//
LOCUS       DAZBWR010000250         1088 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000250 DAZBWR010000000
VERSION     DAZBWR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.259"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1088
                     /locus_tag="ACHGT5_002959"
     rRNA            <1..>1088
                     /locus_tag="ACHGT5_002959"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          287 a          250 c          332 g          219 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagc
//
LOCUS       DAZBWR010000251         1077 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000251 DAZBWR010000000
VERSION     DAZBWR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.260"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..614)
                     /locus_tag="ACHGT5_002960"
     CDS             complement(<1..614)
                     /locus_tag="ACHGT5_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN7218860.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELV"
     gene            complement(690..836)
                     /locus_tag="ACHGT5_002961"
     CDS             complement(690..836)
                     /locus_tag="ACHGT5_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFN7218861.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(940..>1077)
                     /locus_tag="ACHGT5_002962"
     CDS             complement(940..>1077)
                     /locus_tag="ACHGT5_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN7218862.1"
                     /translation="YFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
BASE COUNT          416 a          173 c          111 g          377 t
ORIGIN      
        1 accaattctt ttcttttcaa ttgctgttca tattgttctc gttgcctaat agttattcta
       61 ataaataaaa atagaacaac aaacatttga atgattaaaa atattaagac accaaacaca
      121 aaatgatcaa atacttgata ttgatgagca acataagaaa caaataaacc aattgtaagt
      181 aaataaatca tgaatatagc agtaagttta gaactggact gttcaacaat cctatttata
      241 gctaattttc tatataacca aataaacatc agcaaaaaca aagaatcaag cattatctgg
      301 aatactacat aaccataccc ttttatccct tcaaatgaaa acatatacgt cataaccatg
      361 gaagtaataa tagatctgaa atcatcaata agcatacata atagtaattt atttaaaagc
      421 actatattag ttttcttctt ttcctttaat ataaaataac ttccgattat aggtatagct
      481 gcggaaacat cagtaaataa ttcaacaaaa aaagtgagtg gtatcaataa acatacaaaa
      541 agatattttc ttttgttaag atcagtatta gtcatataaa ataagagaaa aaagaaaaag
      601 tttcccacga acattcttaa tagataagct tttgtatcga tagtaatcaa gagatcccct
      661 cctgatttta ggatatcata aaaaaaattt tagttacact ttttaaataa tgaaaaaaca
      721 cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc tcctttaatt
      781 tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc ttccattacg
      841 attcccccta caaactataa atacttttat gtcactgatt caatatacct atttttctaa
      901 tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag atttatctcc
      961 ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac tcaaatttct
     1021 tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag taaaata
//
LOCUS       DAZBWR010000252         1059 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000252 DAZBWR010000000
VERSION     DAZBWR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.261"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACHGT5_002963"
     CDS             complement(37..993)
                     /locus_tag="ACHGT5_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218863.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAZBWR010000253         1045 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000253 DAZBWR010000000
VERSION     DAZBWR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.262"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHGT5_002964"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHGT5_002964"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN7218864.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAZBWR010000254         1045 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000254 DAZBWR010000000
VERSION     DAZBWR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.263"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1005
                     /locus_tag="ACHGT5_002965"
                     /pseudo
     CDS             370..1005
                     /locus_tag="ACHGT5_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          358 a          188 c          188 g          311 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta ccttatatta tttgtcaaat
     1021 taagcgagac aaaactttcc attta
//
LOCUS       DAZBWR010000255         1031 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000255 DAZBWR010000000
VERSION     DAZBWR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.264"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..845)
                     /locus_tag="ACHGT5_002966"
     CDS             complement(<1..845)
                     /locus_tag="ACHGT5_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218865.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLENINETVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLD"
BASE COUNT          327 a          186 c          105 g          413 t
ORIGIN      
        1 aaatcaagtc ctctacattt taaaggttca cttaaactat taatatagtt aataatgttg
       61 tctatccttt tctctttatc ttcaccttca atctggggag catatagtcc aatttcctcc
      121 caataatatg aatctctttc aattcccatg atggtagctt tggctaattt caaatcaaat
      181 aacgatttta gaattcttag tgtctcaata aagtcttctc tcttcaactc tttaatccat
      241 tgtgaatttt ctggattatc taaaatttca agttcatctt cattataagt atatttcaaa
      301 tttgacttgc ttaactcata gataaatgaa gtttcttgac tcataattct tttaagtact
      361 tgactataag tttcgtttga ctttgcccat ttcgctaatt ctaccactgt attcactaat
      421 aaatcgaaat aatgatgttc tcgttcaata gtctcagcat tttgaaaatg aaaatcacct
      481 ttataaactc cattcttttc cactgtttca ttaatatttt ctaaaaacca gaatgagttt
      541 gcaaatttag tcgtactttt tcgattaact aagttaataa ctcttccatg taataataag
      601 acaaaaatac ataaattttt agcttcctta ttattagata ctaattgatc aatcataggg
      661 agccattttt tgattttcac taattgattg ctcataaatc tttcactgga cttttgttca
      721 atcttaaatt gcaaaacttc taattcataa tttccatctt ctgatttatt tttgggaaca
      781 actatcaaac ttttataaag tttatccaca tcaaaattta tcttttttgc gtaatttgta
      841 gccattaata catctcctta ttcataataa atttcatatg acgctcctct caaaatttac
      901 cggtagacta gaaaaaagaa taaaagatac gtcaattcac actttattct taaaaaaaac
      961 tggtctctct accggtaata tcgattgagt ccgcctttat cgactttatc ctttttttat
     1021 attaaatgtt t
//
LOCUS       DAZBWR010000256         1018 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000256 DAZBWR010000000
VERSION     DAZBWR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.265"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..487
                     /locus_tag="ACHGT5_002967"
     CDS             77..487
                     /locus_tag="ACHGT5_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN7218866.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            501..857
                     /locus_tag="ACHGT5_002968"
     CDS             501..857
                     /locus_tag="ACHGT5_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN7218867.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          350 a          153 c          199 g          316 t
ORIGIN      
        1 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
       61 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
      121 agcaagagca tggttgaaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
      181 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
      241 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
      301 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
      361 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
      421 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
      481 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
      541 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
      601 aagaacgcga tttaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
      661 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
      721 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
      781 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
      841 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
      901 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
      961 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatag
//
LOCUS       DAZBWR010000257         1009 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000257 DAZBWR010000000
VERSION     DAZBWR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.266"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..930
                     /locus_tag="ACHGT5_002969"
     CDS             85..930
                     /locus_tag="ACHGT5_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN7218868.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          389 a          122 c          175 g          323 t
ORIGIN      
        1 ttaaattagg tataattagt atatgttttg tgagaaattt agacttacaa taagcagtga
       61 atttataatc aacgtatagg agacatggat gaaatgaaac taattggtat agatttagac
      121 ggtactcttt tgaattctga ccagagcatt agtcaaaaaa atgcaagaac aatgaaacat
      181 ttaccggaag actgttttcc attcatttgt tctggaagag aagtagaaga cattaataat
      241 attctcaaaa aatctggttt aaggatacca gcagtagggt taaatggttc tgttggattt
      301 gatggagaaa aaaagatatt tgagtcttcc ttcgataaaa atgatgtaaa aaaaattgat
      361 tctgtcctat cgaaatttcc aaccaaaata tatacaaatc tcggaagtta tgaatctcag
      421 aattataaaa ataaaatgaa aaaaattttt cacgaggtag ggagtgaatt ttctatcaaa
      481 gagctaaact atgagcttga gtatgaaaaa tctatttgtt caatccctta taataatatt
      541 gaggaaatta tagaaaagga tgctatcagc atttacaaat tttttatttt tattcctaat
      601 cgtcaattga aagctaatat atttaaccat ttgaaaaaga tgctaaatgt gtctgttact
      661 gaatcggcac ctgtaaatat tgaaatcgta cctcaaaatg tatctaaagg tgtggtgttt
      721 gatcgtttgg aatcaatcta caatttaaag aaacctttga gaatcgcgat cggagatagt
      781 ttaaatgact tatctatgtt tgaaagtgca gatattagtt ttgcaatggg aaatagtcat
      841 caagaaatca tgaaaaaagc tgattatgtc actgtttcaa atgataaaga cggagtatct
      901 caagcatttg aaagaattct taccatataa actaattaag gtagtataga acatagaatt
      961 ctaaatacgg aaaaatggag cataaattcc agtacaaata gtacttctt
//
LOCUS       DAZBWR010000258         1001 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000258 DAZBWR010000000
VERSION     DAZBWR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.267"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHGT5_002970"
     CDS             <1..459
                     /locus_tag="ACHGT5_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218869.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACHGT5_002971"
     CDS             473..904
                     /locus_tag="ACHGT5_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218870.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..>1001
                     /locus_tag="ACHGT5_002972"
     CDS             906..>1001
                     /locus_tag="ACHGT5_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN7218871.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT          373 a          146 c          212 g          270 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga a
//
LOCUS       DAZBWR010000259          996 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000259 DAZBWR010000000
VERSION     DAZBWR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.268"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>996
                     /locus_tag="ACHGT5_002973"
     CDS             <1..>996
                     /locus_tag="ACHGT5_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218872.1"
                     /translation="VKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINRALSLESIF
                     SSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHY
                     VMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSK
                     KESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVT
                     LLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLL
                     ICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFM"
BASE COUNT          316 a          137 c          137 g          406 t
ORIGIN      
        1 tgtaaaaata tttatagttt atgcatttgt tagtttatta tatttatttc cagcatttaa
       61 aaacatggaa atatttggct tttatgatac ggcatttcac attaatcgtg cgttaagttt
      121 agaatctatt tttagctccc ctattaattt tgaaacgttt agatcatatg gcatgcaagt
      181 aaataatttt tacccatggt taactctata tcctttattt ttattgatca agtttactaa
      241 tttagcaata ggctataacc tttttcttta tattgttacg ctcattactc tatttatatg
      301 tcattatgta atgtatgaaa ttaccaaaaa acatgttact agctcctttt tcgctattat
      361 atatacaaca tcttctttta ggagtgtaga aatatttctt cgaggggcaa tgggggaatt
      421 actcgcaatg tcaatacttc ctcttatttt actcgggttt ataaagcttt acgactcaaa
      481 aaaagaatct tgggttatgt tggcgattag catgacttta ttaatttaca ctcatgttct
      541 ttctgtagct atgactatgg taatgattat aattgcttta attattcaat tttatcggaa
      601 aaagaaaata gaaatcttct taattattaa attaataaaa gctgctatag ttactttact
      661 tctttcacta gcgtttatag gtcctatgat tgagcagaca ttgtatcaag atctgaatag
      721 accttatgtg gatatattac aaaaaagagc aatttatctg ggtaaagagt ttttgattgg
      781 aagtatagat tcagaattac tatcatttgg tattggctta actttgctta tttgtttaat
      841 tatactaaca tttaacttta aaaaattagg aatcttatct aaaatcactt atataatggg
      901 tataatatct attctacttt gtacaaaaat atttccatgg tttgccttac aaaatactcc
      961 tttaaatatt attcaagctc cttggcgttt tatgat
//
LOCUS       DAZBWR010000260          989 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000260 DAZBWR010000000
VERSION     DAZBWR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.269"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..891
                     /locus_tag="ACHGT5_002974"
     CDS             <1..891
                     /locus_tag="ACHGT5_002974"
                     /inference="COORDINATES: protein motif:HMM:NF016309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFN7218873.1"
                     /translation="SYNRKIENLKNNQQKIKHFYAYILEFLDTPVMKNISSRRPINVP
                     KSLVMNPKYNTIYKMYLKSRNNRNTLNFSNRYQYYWKSTDVLYEIWCFIKIIRSLSLQ
                     GYTPISGWIFENDILSQELPFLHEDTTVVLSKNDIRINLVYNNSMEKEYFSNTLDIPV
                     KTSSKRNKPDIRLDIILQDKYYIGSLIFEVKYKKLDNILDNDKGRQRQQLMAYKQNTM
                     SSILAFPEILTRSLQAVSAVFALYPSNEGRKKTAPKYYEKEGIFFHLLNPSSDEKELS
                     VKIQTSIEERISLFNQQSRN"
BASE COUNT          398 a          142 c          124 g          325 t
ORIGIN      
        1 agctataata gaaagattga aaatttgaaa aacaatcagc aaaagataaa acatttttat
       61 gcttatatcc ttgaattttt agacacacca gtaatgaaaa acatttcatc aagaagacct
      121 ataaatgttc caaaatcact tgttatgaac cctaaatata acactatata taaaatgtat
      181 ttaaaaagta gaaacaatcg taatacttta aatttttcaa atagatatca gtattattgg
      241 aaaagtacag atgtactcta tgaaatttgg tgctttataa aaattattag atctctatcc
      301 ctgcaaggct acactccaat ttctggatgg atttttgaga atgatattct ttcacaagaa
      361 ttgccgtttt tacatgaaga cactaccgta gtattgagta aaaatgatat acgtattaat
      421 cttgtatata ataattccat ggaaaaagaa tatttctcca atacactaga tatcccagta
      481 aaaacctctt caaaacgaaa taaacctgat attagattag acattatcct tcaagataaa
      541 tactatatcg gaagcttaat atttgaagta aaatataaaa aactagacaa tatacttgat
      601 aatgataaag gtaggcaacg tcaacaatta atggcttata aacaaaatac tatgtcaagt
      661 attctagcat ttcctgaaat tcttacacgt tctttgcaag ctgtctctgc tgtctttgct
      721 ttatatcctt ccaatgaagg aagaaaaaaa actgcaccta aatattatga aaaagaaggt
      781 attttttttc acttattaaa tccttcatca gatgaaaaag agctttcagt aaaaattcaa
      841 acaagtattg aagaaagaat ttcattattt aatcaacaaa gtagaaatta aaaagatgaa
      901 gagcgtctct ttatacaaga gagcgctctt catcttcaat tttttgtaac aaatttgaat
      961 acataataaa gttattatta tttttttga
//
LOCUS       DAZBWR010000261          982 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000261 DAZBWR010000000
VERSION     DAZBWR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.270"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>982
                     /locus_tag="ACHGT5_002975"
     CDS             82..>982
                     /locus_tag="ACHGT5_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN7218874.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEY"
BASE COUNT          335 a          183 c          192 g          272 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata ca
//
LOCUS       DAZBWR010000262          962 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000262 DAZBWR010000000
VERSION     DAZBWR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.271"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..589
                     /locus_tag="ACHGT5_002976"
     CDS             50..589
                     /locus_tag="ACHGT5_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218875.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            586..924
                     /locus_tag="ACHGT5_002977"
     CDS             586..924
                     /locus_tag="ACHGT5_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFN7218876.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT          348 a          128 c          181 g          305 t
ORIGIN      
        1 atttagaaag cgttttcctc aaaatttaat gaattaggaa gtgattacta tgaaaaaatt
       61 atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt ctggaaatat
      121 agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt ttagtcaatt
      181 aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag atacttttta
      241 cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg tattgcggtt
      301 aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata ttataacaag
      361 tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg caaaatcata
      421 tactcaaaca gtaaatctag ctaattctta tggttatggt ggaggaatgt attttacatt
      481 tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata atacatttgg
      541 tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat aatttcttac
      601 tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt acatgaggat
      661 acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac aaatttatat
      721 tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa cttgttgcag
      781 acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt aaaatatggc
      841 gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag tattcaggag
      901 gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga atttgacatt
      961 ta
//
LOCUS       DAZBWR010000263          943 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000263 DAZBWR010000000
VERSION     DAZBWR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.272"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHGT5_002978"
     rRNA            <1..>943
                     /locus_tag="ACHGT5_002978"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZBWR010000264          920 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000264 DAZBWR010000000
VERSION     DAZBWR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.273"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..>920)
                     /locus_tag="ACHGT5_002979"
     CDS             complement(56..>920)
                     /locus_tag="ACHGT5_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN7218877.1"
                     /translation="GKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPEL
                     EEWQARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTE
                     SKNRWMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRY
                     LPSKDYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFD
                     YGSAIRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFM
                     KTNARSDSYDV"
BASE COUNT          285 a          194 c          163 g          278 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agacatcatc cataaaatgg actttgtcta cagtctgcgc attctcatta gagaacactc
      181 ccttttttgt gacttttctg aagctagagt gaatactttc aactgcatta gtagtgtaca
      241 tgatttttct aatggcgctg ccataatcaa agagttgttc aacatgagaa aagtttcgtt
      301 tgcatacatc tactgcacca gaataatgag accaccgatt ttgaaaactg tcgaaggcag
      361 cgtgtgcagc gtttaaagaa gaagcactgt agaatttttt catgtctcga cagacttcct
      421 tataatcctt acttgggagg tatcgtaacg catttcgaac aagatgaaca atacagcgct
      481 gcacaatgac agaaggaaaa attgcttttg cgccttcttt gagaccggaa acaccgtcca
      541 tcgaaatgaa aaaaacatct tctacaccac gtactttcag ttcatcaaat acttgcatcc
      601 agcgattttt agattctgtt tgattgagcc ataggcctaa aatctccttg tttcctttaa
      661 gatcatagcc aagaatggtg tacacagcat attctttagc ttcatagttt tcgcgtaaag
      721 tgacgtacat acaatcaacg aatagaaatg cataacactt ggctaatggc cgagcttgcc
      781 attcttccaa ttcaggaagg acggcatccg tgatatcaga aatcatttcg tgggaaatat
      841 caaagccgta aatatcttca acggttgcgg cgatatcgca ttgactcatt cctcgtgcat
      901 acatggaaag aaccttacct
//
LOCUS       DAZBWR010000265          908 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000265 DAZBWR010000000
VERSION     DAZBWR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.274"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..740
                     /locus_tag="ACHGT5_002980"
     CDS             48..740
                     /locus_tag="ACHGT5_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN7218878.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          400 a          143 c          151 g          214 t
ORIGIN      
        1 tcacttactt ttagcatgta aaagtattgg attaaggagc tatgcaaatg aaaaagaaat
       61 ttattactgg tgggattgtc gttgcatttc tatgtctaat tggctacaca ggcacgcggt
      121 acgcaaatga aattattttg tggggtggag agacagatat tgatacaatc aatgagaatc
      181 tgacacagct tgatcataca ttgacagata aagagcaagc gattacagat cttaccgctc
      241 gactagcaca aaaagaacag acattgcaag attcaaaggc agatgtccaa gaataccaaa
      301 caaaagtaaa tgaattagaa aaccaaaaaa atcaatcggc agcagaaaaa caaaacttag
      361 aaagtcaatt agtcagcaag gatgcacaac tacaaagcaa acaaaatgaa atcaatgtca
      421 aactagatga gatcaaccaa aaaaatgatg aaatcaatca tctgaatgca gattggacag
      481 caagactaca agaagcacaa gcaaaacttt cagaattaca aaatactgta aatggtttga
      541 atcaacaaat cgcaacactg acacaagaaa aaacaaatgt agaaactcag ttaacagata
      601 cacaaaagaa attagacgaa gcaactgctg aaaactcaag cttgaaacaa tatattcaaa
      661 agttagaaaa agcaaaagat gaagtggaag atacagctga taaatctcag caaattgttg
      721 aagaacattt gcgtaaataa tctttcttaa caagtaaacc taaattagta aagaagcaac
      781 agtaatctat actaagtagt tcagttttgg agtacaaaat gacaaaatgt acttcaaaat
      841 ggactacttt tttcatttta acttacatac caaattacat acaaaatgat ttataaaatg
      901 caattgaa
//
LOCUS       DAZBWR010000266          905 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000266 DAZBWR010000000
VERSION     DAZBWR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.275"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..905
                     /locus_tag="ACHGT5_002981"
                     /pseudo
     CDS             1..905
                     /locus_tag="ACHGT5_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          330 a          157 c          172 g          246 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacac
//
LOCUS       DAZBWR010000267          814 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000267 DAZBWR010000000
VERSION     DAZBWR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.276"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHGT5_002982"
     CDS             136..309
                     /locus_tag="ACHGT5_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218879.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          272 a          131 c          103 g          308 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgtt
//
LOCUS       DAZBWR010000268          800 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000268 DAZBWR010000000
VERSION     DAZBWR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.277"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACHGT5_002983"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACHGT5_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          287 a          125 c          127 g          261 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
      781 ctttattcta tgctttatat
//
LOCUS       DAZBWR010000269          794 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000269 DAZBWR010000000
VERSION     DAZBWR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.278"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACHGT5_002984"
     CDS             <1..367
                     /locus_tag="ACHGT5_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN7218880.1"
                     /translation="FGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFG
                     RVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGK
                     ENWNDLYHANEVLKIAEEK"
     gene            368..>794
                     /locus_tag="ACHGT5_002985"
     CDS             368..>794
                     /locus_tag="ACHGT5_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN7218881.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSV"
BASE COUNT          309 a           87 c          140 g          258 t
ORIGIN      
        1 atttggtttc cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc ttgaaattaa
       61 cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag aaattcattt
      121 agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag atgaatgtat
      181 caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta aagaaatgcc
      241 tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa acgtagtaac
      301 aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa tagctgagga
      361 gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg ggtttaaaaa
      421 taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag caatgataga
      481 tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata cggacagtaa
      541 tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc
      601 tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat tcttacaact
      661 agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt cacctttacg
      721 tgaagtttct aatcttgaag aattaataga tgaacatcta aaaaatttag ataaagacgt
      781 ctacttttca gttg
//
LOCUS       DAZBWR010000270          787 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000270 DAZBWR010000000
VERSION     DAZBWR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.279"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..777)
                     /locus_tag="ACHGT5_002986"
     CDS             complement(<1..777)
                     /locus_tag="ACHGT5_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN7218882.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRL"
BASE COUNT          264 a           92 c          142 g          289 t
ORIGIN      
        1 gagacgtttt gctttttcca tttttagttc tttaaaatag ctaataacac tttttccatt
       61 cacttttcta aaatgttttt ttaagctggc tggactgata aaaaaaattt gggctaattg
      121 ttcaagcaaa aaattttctg aaagatgttc ttctaaatat cgtatgattg gatcaactaa
      181 ttcatctgaa taaagaatat gctttttttt aggaatgact ttctcatttg aaggtattgt
      241 caataaagca agttcctgtt gataatgcct tggtaaggaa gacaattgaa ttttttgcag
      301 acaataagta actaataagg aaagcaaatg aatgctcatc tgttggcata gtatgaaaaa
      361 agaattgatt tcctgatcat aggaaatagc tggcttacta tgttttagat aatcaatttt
      421 gaactgttca tttttcagaa aagtaatgat agatgttatt tcttgatcta tagaaaaaga
      481 ttgtcccgta atatgtgcta ataattgctc atcaaaataa aactggataa tgataaaagt
      541 ttgtgtttcc ttttgagtag aaaagatttg gacttcttgc tgtttatcaa tgaataaaag
      601 ggaaggatta tctagttcta tagatttatg tgcaaattca gcatataaat tagcgctttc
      661 atttattagt agtcgaaaat agggagcagc agataaatga agatgggtat ctatggaata
      721 ggttagtgtt tttttgacaa gattggagga ttggttgttt gttaacaaaa gaaccacttt
      781 cctttct
//
LOCUS       DAZBWR010000271          787 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000271 DAZBWR010000000
VERSION     DAZBWR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.280"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACHGT5_002987"
     CDS             <1..716
                     /locus_tag="ACHGT5_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN7218883.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DAZBWR010000272          784 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000272 DAZBWR010000000
VERSION     DAZBWR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.281"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..126)
                     /locus_tag="ACHGT5_002988"
     tRNA            complement(54..126)
                     /locus_tag="ACHGT5_002988"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91..93),aa:His,seq:gtg)
     gene            complement(129..202)
                     /locus_tag="ACHGT5_002989"
     tRNA            complement(129..202)
                     /locus_tag="ACHGT5_002989"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Trp,seq:cca)
     gene            complement(209..289)
                     /locus_tag="ACHGT5_002990"
     tRNA            complement(209..289)
                     /locus_tag="ACHGT5_002990"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Tyr,seq:gta)
     gene            complement(291..366)
                     /locus_tag="ACHGT5_002991"
     tRNA            complement(291..366)
                     /locus_tag="ACHGT5_002991"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(331..333),aa:Phe,seq:gaa)
     gene            complement(402..474)
                     /locus_tag="ACHGT5_002992"
     tRNA            complement(402..474)
                     /locus_tag="ACHGT5_002992"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Asp,seq:gtc)
     gene            complement(484..556)
                     /locus_tag="ACHGT5_002993"
     tRNA            complement(484..556)
                     /locus_tag="ACHGT5_002993"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(521..523),aa:Val,seq:tac)
     gene            complement(572..643)
                     /locus_tag="ACHGT5_002994"
     tRNA            complement(572..643)
                     /locus_tag="ACHGT5_002994"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(608..610),aa:Glu,seq:ttc)
     gene            complement(658..747)
                     /locus_tag="ACHGT5_002995"
     tRNA            complement(658..747)
                     /locus_tag="ACHGT5_002995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(711..713),aa:Ser,seq:gga)
BASE COUNT          215 a          215 c          182 g          172 t
ORIGIN      
        1 agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat
       61 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
      121 attcgccatg gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt
      181 tctaccttta aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct
      241 aaggaacgga tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg
      301 ggacagaatc gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac
      361 cgcgccatat tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga
      421 acccgcgatc tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca
      481 ttatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
      541 gctgagctaa acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta
      601 ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg
      661 agagtaaggg attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg
      721 ctccttcggc ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa
      781 tgag
//
LOCUS       DAZBWR010000273          771 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000273 DAZBWR010000000
VERSION     DAZBWR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.282"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="ACHGT5_002996"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="ACHGT5_002996"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          251 a          126 c          150 g          244 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta a
//
LOCUS       DAZBWR010000274          758 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000274 DAZBWR010000000
VERSION     DAZBWR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.284"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>758
                     /locus_tag="ACHGT5_002997"
     CDS             <1..>758
                     /locus_tag="ACHGT5_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN7218884.1"
                     /translation="INFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGT
                     LSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSY
                     LGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFT
                     LITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFI
                     AIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFT"
BASE COUNT          236 a           97 c          137 g          288 t
ORIGIN      
        1 gattaacttt aaccatttta aatatttgat tttatctttt atatctactt ttttgaatat
       61 tgcaatctct gtagctttga tgcagacatt atattttgat gataaatatg ttggcagagt
      121 agtcggcgga acactgtcat ttgtgttgat aggtttagta atttatttaa gaataatgat
      181 aaagggtgct catttttttg ataaaaaaca gtggagatac gctttaaaaa tctctttgcc
      241 gctaatccca cattccttag caaatatagt tttagcacaa ttcgatagaa ttatgattaa
      301 ttcttattta ggtcctgctg cggggggaat atattcctac attagtaaca taggtattat
      361 tctaagcgta atatggatgt ctactaataa cgcctgggtt ccatggttct atggtgagat
      421 gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac tattatttag tagtctttac
      481 tttgattacg atgattgtaa tggtagtttc gattgattta gcaaaattta tggctccgaa
      541 agactattat tctgggttat ctttagtggt tccgataacc ttaggctatt tctttcagtt
      601 tttatatagt ttaccagtta atgctgaatt ttatgagaaa aaaacatcgt ttattgcgat
      661 tggtacagta gcaagtgcta tagttaatat actattaaac atgattttca tacctaggtt
      721 aggaataatt gctgctggat acacaaccgt atttacgt
//
LOCUS       DAZBWR010000275          742 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000275 DAZBWR010000000
VERSION     DAZBWR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.285"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHGT5_002998"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHGT5_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>742)
                     /locus_tag="ACHGT5_002999"
     CDS             complement(570..>742)
                     /locus_tag="ACHGT5_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN7218885.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT          228 a          136 c          116 g          262 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt ga
//
LOCUS       DAZBWR010000276          655 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000276 DAZBWR010000000
VERSION     DAZBWR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.286"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACHGT5_003000"
     CDS             <1..360
                     /locus_tag="ACHGT5_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218886.1"
                     /translation="TLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPI
                     STISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKIL
                     LPENWAELRKLVEYGII"
     gene            347..>655
                     /locus_tag="ACHGT5_003001"
     CDS             347..>655
                     /locus_tag="ACHGT5_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN7218887.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     I"
BASE COUNT          220 a           97 c           93 g          245 t
ORIGIN      
        1 actttaatta agcttcatcc tagagcaagg acaaatcgtt atggtgaaaa aaacatattg
       61 aattccagtt taccgtttga agtcttgcta cttgaaaatg acttctcttc aacacttttg
      121 attagtccta tatctacaat ttctttttca ccatctatga tgtttgaaaa gacgatcaac
      181 tctattatgt tggctaaatt aattttaaat gaatatacag cattatctga agataaaaaa
      241 gaaacattga caaatattgt tcttttctgt gaacattata accgtctatc agaagaaaaa
      301 attttactcc cagaaaactg ggcagaatta agaaagttgg tagagtatgg gattatttaa
      361 cacactttcc aagtcaaaat tagttaagtc cagtttctgg tatactatag ggaatttttt
      421 tattaaggga atttcattca taactattcc tatttttgta aatattacta ctcaagcaga
      481 atatggtttg attaataatt tcacttcatt tgtatcaatt ttttcgattc ttgttgggct
      541 aagcctaaat tcggcaatta ataatgcaag ttttgactat aaagatgaca taaatgaatt
      601 tatgtcatcg gtactttctc tagctacact ttcgttaatt gtgttcttat taata
//
LOCUS       DAZBWR010000277          636 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000277 DAZBWR010000000
VERSION     DAZBWR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.287"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..428)
                     /locus_tag="ACHGT5_003002"
     CDS             complement(<1..428)
                     /locus_tag="ACHGT5_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN7218888.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFV"
BASE COUNT          223 a          103 c           89 g          221 t
ORIGIN      
        1 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
       61 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
      121 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
      181 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
      241 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
      301 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
      361 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
      421 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
      481 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
      541 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc taataaaaat
      601 cttagaataa aaatttttac tgatttaatt tagaat
//
LOCUS       DAZBWR010000278          635 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000278 DAZBWR010000000
VERSION     DAZBWR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.288"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..522)
                     /gene="panD"
                     /locus_tag="ACHGT5_003003"
     CDS             complement(127..522)
                     /gene="panD"
                     /locus_tag="ACHGT5_003003"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFN7218889.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(532..>635)
                     /locus_tag="ACHGT5_003004"
     CDS             complement(532..>635)
                     /locus_tag="ACHGT5_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFN7218890.1"
                     /translation="LQRVNYIEKSVLVAIAVFIGKTRLIDNFTFELQ"
BASE COUNT          199 a          126 c           50 g          260 t
ORIGIN      
        1 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
       61 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      121 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      181 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      241 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      301 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      361 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      421 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      481 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      541 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      601 aactgatttt tcaatataat ttactctttg taatg
//
LOCUS       DAZBWR010000279          632 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000279 DAZBWR010000000
VERSION     DAZBWR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.289"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..607
                     /gene="spxA"
                     /locus_tag="ACHGT5_003005"
     CDS             209..607
                     /gene="spxA"
                     /locus_tag="ACHGT5_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN7218891.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          225 a           95 c          120 g          192 t
ORIGIN      
        1 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
       61 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
      121 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
      181 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
      241 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
      301 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
      361 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
      421 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
      481 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
      541 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
      601 attatagaag gataatacga aacttggaaa ag
//
LOCUS       DAZBWR010000280          629 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000280 DAZBWR010000000
VERSION     DAZBWR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.290"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..>629)
                     /locus_tag="ACHGT5_003006"
     CDS             complement(103..>629)
                     /locus_tag="ACHGT5_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218892.1"
                     /translation="LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKNIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          205 a           95 c           69 g          260 t
ORIGIN      
        1 agtcaatttg cttttcacta attacaaaat atgtgatata ggaagtttag ttattgttct
       61 cataatgcca tcctgttttt agattatcta gtaatttttt tgttatttaa aattaggagc
      121 agtgccaatt gttttaattt taaaggcttt ttttatatct tctaaattag aagatatttg
      181 tatatcaata tggaatatat tacctttttt gtattttttt atgttatctt tagctaaaac
      241 atcaaataca tgtccttgaa attgtatcga ggcagctgga tttagtttaa attgaggaaa
      301 ctttaccaca gctgattcaa aacacttgtc acattttttt atatttttac ctttgtaaaa
      361 atattcgatt gaatattcta tttggcacga ctcacttggc agtatatgta tgtcaatatt
      421 tttcatctgt ttcataggta tttcaacatc taaactatta aaaacatata attccctgta
      481 attatttaca tctaactcta ctaaatttat ctttggaaca atagatcctc cacccattcc
      541 accataataa aaaaaatctt gttctctatc aaaatagcac atattaagct cagtttgttt
      601 atcatcctca ataattttat aaatcaaac
//
LOCUS       DAZBWR010000281          584 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000281 DAZBWR010000000
VERSION     DAZBWR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.291"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>584)
                     /locus_tag="ACHGT5_003007"
     CDS             complement(39..>584)
                     /locus_tag="ACHGT5_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN7218893.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          101 g          206 t
ORIGIN      
        1 gaaatccact ccttttttca tgctctaaaa tgatattttt acacaactgt tacggttgtt
       61 ttacgaaatt ggacgggact ttgccagttt aatttctgtt ttatccgttc attattgtag
      121 taatagatat agctatcgat ggctgatttt aattcctcga aacagttata aactttcccg
      181 tgaaaaatct cttgttttaa taagccaaaa aaattctcca taagtgaatt atctaagcag
      241 ttccctttcc gagacatgct ttgaaagatt ttgtactcct tcaatttgtt tctataagct
      301 ttcatttgat acgcccaacc ttgatctgag tgaaacgtac gacgataggg acaatcgtta
      361 gtggtctgga tagcttcctc caaaccttcc attaccgcta atgcattggg cttttcagag
      421 atacgatagg ataaaatctc actattgaac atatccataa aaggatctag atacaatttc
      481 ttttgacgga taatgccttt ggcatccact tcatggtatt taaattcagt tgtatccgtc
      541 gtgattttct gatggcagat actggtgtag aaacgacgat ggat
//
LOCUS       DAZBWR010000282          576 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000282 DAZBWR010000000
VERSION     DAZBWR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.292"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..>576
                     /locus_tag="ACHGT5_003008"
     CDS             80..>576
                     /locus_tag="ACHGT5_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN7218894.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          217 a          106 c           93 g          160 t
ORIGIN      
        1 aaaaatccat acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca
       61 cagaaaagag gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac
      121 taaaggcgat ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct
      181 acggactgaa ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc
      241 aggtaattcg agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac
      301 attggaaata cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa
      361 aagaacaaac gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat
      421 gtctgaaatt gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat
      481 gtccaacatg actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa
      541 tgataagtat gtcgctattt ttatggacgc tactta
//
LOCUS       DAZBWR010000283          575 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000283 DAZBWR010000000
VERSION     DAZBWR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.293"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..575
                     /locus_tag="ACHGT5_003009"
                     /pseudo
     CDS             258..575
                     /locus_tag="ACHGT5_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAZBWR010000284          575 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000284 DAZBWR010000000
VERSION     DAZBWR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.294"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DAZBWR010000285          569 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000285 DAZBWR010000000
VERSION     DAZBWR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.295"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACHGT5_003010"
     rRNA            <1..>569
                     /locus_tag="ACHGT5_003010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAZBWR010000286          534 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000286 DAZBWR010000000
VERSION     DAZBWR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.296"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHGT5_003011"
     CDS             <1..>534
                     /locus_tag="ACHGT5_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN7218895.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZBWR010000287          514 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000287 DAZBWR010000000
VERSION     DAZBWR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.297"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHGT5_003012"
     CDS             complement(<1..132)
                     /locus_tag="ACHGT5_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN7218896.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZBWR010000288          508 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000288 DAZBWR010000000
VERSION     DAZBWR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.298"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DAZBWR010000289          476 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000289 DAZBWR010000000
VERSION     DAZBWR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.300"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>476
                     /locus_tag="ACHGT5_003013"
     CDS             22..>476
                     /locus_tag="ACHGT5_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218897.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DAZBWR010000290          476 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000290 DAZBWR010000000
VERSION     DAZBWR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.301"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..>476
                     /locus_tag="ACHGT5_003014"
     CDS             125..>476
                     /locus_tag="ACHGT5_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN7218898.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          180 a           78 c           86 g          132 t
ORIGIN      
        1 ccgcactatg ccattacatt tatatctacg cgaattgtaa aattaagtta gaaaaataaa
       61 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
      121 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
      181 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
      241 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
      301 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
      361 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
      421 gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttg
//
LOCUS       DAZBWR010000291          470 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000291 DAZBWR010000000
VERSION     DAZBWR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.302"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          152 a           90 c           55 g          173 t
ORIGIN      
        1 gcgaaagcac aaaaaataaa aaaattatta cgttattaaa aaaacagatg aaagatcagg
       61 ttttattaga tctcattctt tcatcacttg aagggattta atgaactact ttttcctctt
      121 tgtcgttttt atatattcgt attccttcaa acagtaggat agcctctttc aatgcttgat
      181 atttgaactg tccgttactt aaaaatttag atgtgccgcc ctcttcctag aacatattct
      241 tttttacttt ttcattaatt gacagaccaa tcgaacgaaa ctgtaaattt cgataggata
      301 atgattggtc aaactcattc attttcaaga catctcgatt catattcaag ataggaatct
      361 ttttatcaac atcactttat tcgtattttc ctctaccata aaagcttcac gctccacaaa
      421 attgttttat acactactat tcagcccatt caacgaccaa tgaatttttt
//
LOCUS       DAZBWR010000292          433 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000292 DAZBWR010000000
VERSION     DAZBWR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.304"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          156 a           61 c           53 g          163 t
ORIGIN      
        1 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
       61 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      121 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
      181 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
      241 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
      301 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
      361 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
      421 gataataaag tac
//
LOCUS       DAZBWR010000293          429 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000293 DAZBWR010000000
VERSION     DAZBWR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.305"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..400
                     /locus_tag="ACHGT5_003015"
     CDS             101..400
                     /locus_tag="ACHGT5_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN7218899.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          165 a           51 c           86 g          127 t
ORIGIN      
        1 gttttaacaa tccagtaatg atttaaaaaa atctagatgt agcgtatact ttgtttatag
       61 aatgtagtgg gaagaaatat atttttggga ggaaatcgtg atgtctaaaa gtgaagaaat
      121 agaaaacatg gtactaaaat tatatgatct cgttttaaat ccagagatca aagaaaagga
      181 acgtatactt ttgatagacg caaagacagg cttagaaaaa ggtcagtatt atcctaaagt
      241 cataaataat ttggaacgta gtttacgtcc gttagcaatc aggggagaat tgtctaaacc
      301 agtatcaccg ttttatatgg agatatcaac aataggaaaa tttgaaaaag aattaggtcg
      361 aggaatggct tcggcaccga ttacttttgg gcatttataa aaacgagtta ttcatctcaa
      421 tgaattaaa
//
LOCUS       DAZBWR010000294          401 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000294 DAZBWR010000000
VERSION     DAZBWR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.306"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          172 a           53 c           67 g          109 t
ORIGIN      
        1 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
       61 ttgttgacat gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg
      121 tgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa caaatgataa
      181 gttatgataa atgagttgct gtaagatttc gaaaaacgaa atcaaaaaaa cttttaaaaa
      241 agagttgaca attacttgtc tctctgatat gatataaaag ttgctacggc agccaaaaac
      301 gaatcgggga acactcctga tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc
      361 aaatgtgtag ggcgtcttga ttcaattcaa gacaacaaac a
//
LOCUS       DAZBWR010000295          380 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000295 DAZBWR010000000
VERSION     DAZBWR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.307"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>380
                     /locus_tag="ACHGT5_003016"
     CDS             <1..>380
                     /locus_tag="ACHGT5_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN7218900.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHK"
BASE COUNT          133 a           71 c           62 g          114 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa
//
LOCUS       DAZBWR010000296          368 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000296 DAZBWR010000000
VERSION     DAZBWR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.311"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHGT5_003017"
     CDS             complement(<1..272)
                     /locus_tag="ACHGT5_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218901.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZBWR010000297          346 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000297 DAZBWR010000000
VERSION     DAZBWR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.312"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>346
                     /locus_tag="ACHGT5_003018"
     CDS             <1..>346
                     /locus_tag="ACHGT5_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN7218902.1"
                     /translation="AVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQ
                     IWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLK
                     RLVKQFDEPKVVV"
BASE COUNT          116 a           55 c           72 g          103 t
ORIGIN      
        1 agccgtgggc tactatcttc gttataacct tagctatcgt gaagttcaag aaatcttata
       61 tgatcgtggc attaacgttt ctcatacgac gatttatcgt tgggtgcaag aatatggcaa
      121 actactctat caaatttgga aaaagaaaaa taaaaaatcc ttttattcat ggaaaatgga
      181 tgaaacgtac atcaaaatta aaggaaaatg gcattatttg tatcgagcca tcgatgcaga
      241 tggtttaacc ttggatattt ggttacgtaa aaaacgggac acacaagcag cctatgcttt
      301 tcttaagcgg ttagtgaagc agtttgatga accgaaggtt gtagtc
//
LOCUS       DAZBWR010000298          321 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000298 DAZBWR010000000
VERSION     DAZBWR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.313"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHGT5_003019"
     CDS             complement(<1..>321)
                     /locus_tag="ACHGT5_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218903.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZBWR010000299          302 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000299 DAZBWR010000000
VERSION     DAZBWR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.314"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>302
                     /locus_tag="ACHGT5_003020"
     CDS             <1..>302
                     /locus_tag="ACHGT5_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN7218904.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          117 a           48 c           68 g           69 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 ag
//
LOCUS       DAZBWR010000300          297 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000300 DAZBWR010000000
VERSION     DAZBWR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.316"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          108 a           43 c           49 g           97 t
ORIGIN      
        1 tattgagagg tcgtgaaaaa gccgcttagt ttcaagtatt aagaaatatt ttgtgaaaat
       61 ggcttctcac atttttgcat aaatttgact taataccgag aaactggctt ttgaacaccg
      121 tttattgagc gtctataaac tgacctgagt attagagaaa caaaacggca aagtagctcc
      181 atatactttg ccgttttgtt ttgaactaat accgaagaga agctgatcaa acaacatttc
      241 ttttataata tagattttag acatatatat gatataaaaa tagttttcgc aaaaaaa
//
LOCUS       DAZBWR010000301          289 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000301 DAZBWR010000000
VERSION     DAZBWR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.317"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>289
                     /locus_tag="ACHGT5_003021"
     CDS             <1..>289
                     /locus_tag="ACHGT5_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218905.1"
                     /translation="EYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELFDWAETYDKIKN
                     FHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKADPYS"
BASE COUNT          125 a           39 c           47 g           78 t
ORIGIN      
        1 gaatatgagc aaataaaaaa atatcttgaa gtaattgttc aaacgtcaga tgttgaagac
       61 tttttttata caatcaaaaa atatcgcgat gaattattcg actgggctga aacatatgat
      121 aaaattaaaa atttccacaa gcaaaatagt actcagaaaa aaatctggga gaatgcacaa
      181 gataaaatta gattaatcga aaaaagtcgg cgtcaaactt ctagccaaga aattgatcaa
      241 ttgattcatg agatgaaaac cattttgaaa aaggctgatc cttattcag
//
LOCUS       DAZBWR010000302          271 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000302 DAZBWR010000000
VERSION     DAZBWR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.318"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           89 a           42 c           47 g           93 t
ORIGIN      
        1 gggaagaata aaagagttag gacgggatta tctactattt ttctttgttt taaatatttg
       61 ttatctttga acataaaaga gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat
      121 aaacggtgtt caattagctg atcttgtatt cacttaaaaa atggtaaagt acgagttgct
      181 attccccatt ttcaattttc taacgctcag gacaaaatac tgttttcaca acctcattgg
      241 aataaaagca tatatattgt ttcaagaacg a
//
LOCUS       DAZBWR010000303          266 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000303 DAZBWR010000000
VERSION     DAZBWR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.319"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACHGT5_003022"
     CDS             complement(34..>266)
                     /locus_tag="ACHGT5_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN7218906.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAZBWR010000304          253 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000304 DAZBWR010000000
VERSION     DAZBWR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.322"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHGT5_003023"
     CDS             <1..>253
                     /locus_tag="ACHGT5_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN7218907.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZBWR010000305          252 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000305 DAZBWR010000000
VERSION     DAZBWR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.323"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACHGT5_003024"
     CDS             complement(32..>252)
                     /locus_tag="ACHGT5_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN7218908.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZBWR010000306          250 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000306 DAZBWR010000000
VERSION     DAZBWR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.324"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           78 a           42 c           33 g           97 t
ORIGIN      
        1 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
       61 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
      121 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
      181 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
      241 ttattttaga
//
LOCUS       DAZBWR010000307          248 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000307 DAZBWR010000000
VERSION     DAZBWR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.denovo.325"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>248)
                     /locus_tag="ACHGT5_003025"
     CDS             complement(44..>248)
                     /locus_tag="ACHGT5_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFN7218909.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           63 a           62 c           49 g           74 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttg
//
LOCUS       DAZBWR010000308        17414 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.056, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000308 DAZBWR010000000
VERSION     DAZBWR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.056"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..782
                     /locus_tag="ACHGT5_003026"
     CDS             <1..782
                     /locus_tag="ACHGT5_003026"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFN7218910.1"
                     /translation="DADEDAEAVRELPELCEGQTFADVAASVTEHFTTPPKPYTEDTL
                     LSAMERAGAEDMPEDAERKGLGTPATRAAILEKLVQMGFVQRKGKQLIPTKDGINLAV
                     VLPEALTSPQLTAEWENRLTEIAKGQADPDEFMDGIETQARELVQTYSCISEEKQKLF
                     QAERVPIGTCPRCGEAVYEGKKNYYCGNRACQFVMWKNDRFFEERKKAFTPKIAAALL
                     KTGKVKVKGLYSVKTGKTYDGTVLLADTGGKYVNYRIEHRS"
     gene            931..4851
                     /locus_tag="ACHGT5_003027"
     CDS             931..4851
                     /locus_tag="ACHGT5_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFN7218911.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            4852..5796
                     /locus_tag="ACHGT5_003028"
     CDS             4852..5796
                     /locus_tag="ACHGT5_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218912.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            5800..6003
                     /locus_tag="ACHGT5_003029"
     CDS             5800..6003
                     /locus_tag="ACHGT5_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFN7218913.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(6303..6539)
                     /locus_tag="ACHGT5_003030"
     CDS             complement(6303..6539)
                     /locus_tag="ACHGT5_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218914.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(6722..7093)
                     /locus_tag="ACHGT5_003031"
     CDS             complement(6722..7093)
                     /locus_tag="ACHGT5_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN7218915.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(7090..8418)
                     /locus_tag="ACHGT5_003032"
     CDS             complement(7090..8418)
                     /locus_tag="ACHGT5_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN7218916.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(8379..8708)
                     /gene="mobC"
                     /locus_tag="ACHGT5_003033"
     CDS             complement(8379..8708)
                     /gene="mobC"
                     /locus_tag="ACHGT5_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFN7218917.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(8966..9337)
                     /locus_tag="ACHGT5_003034"
     CDS             complement(8966..9337)
                     /locus_tag="ACHGT5_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7218918.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            9482..9667
                     /locus_tag="ACHGT5_003035"
     CDS             9482..9667
                     /locus_tag="ACHGT5_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN7218919.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            9825..9992
                     /locus_tag="ACHGT5_003036"
     CDS             9825..9992
                     /locus_tag="ACHGT5_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218920.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            10610..11269
                     /gene="vanR-B"
                     /locus_tag="ACHGT5_003037"
     CDS             10610..11269
                     /gene="vanR-B"
                     /locus_tag="ACHGT5_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN7218921.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            11272..12615
                     /gene="vanS-B"
                     /locus_tag="ACHGT5_003038"
     CDS             11272..12615
                     /gene="vanS-B"
                     /locus_tag="ACHGT5_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN7218922.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            12786..13592
                     /gene="vanY"
                     /locus_tag="ACHGT5_003039"
     CDS             12786..13592
                     /gene="vanY"
                     /locus_tag="ACHGT5_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFN7218923.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            13610..14437
                     /gene="vanW-B"
                     /locus_tag="ACHGT5_003040"
     CDS             13610..14437
                     /gene="vanW-B"
                     /locus_tag="ACHGT5_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFN7218924.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            14434..15405
                     /locus_tag="ACHGT5_003041"
     CDS             14434..15405
                     /locus_tag="ACHGT5_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN7218925.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            15398..16426
                     /gene="vanB"
                     /locus_tag="ACHGT5_003042"
     CDS             15398..16426
                     /gene="vanB"
                     /locus_tag="ACHGT5_003042"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN7218926.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            16432..17040
                     /locus_tag="ACHGT5_003043"
     CDS             16432..17040
                     /locus_tag="ACHGT5_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN7218927.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(17106..17279)
                     /locus_tag="ACHGT5_003044"
     CDS             complement(17106..17279)
                     /locus_tag="ACHGT5_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218928.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         4445 a         4278 c         4708 g         3983 t
ORIGIN      
        1 ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc ggagctttgc gagggccaga
       61 cctttgcaga cgtggccgcc tctgtcaccg agcatttcac cacgccgccc aagccctaca
      121 ccgaagatac gcttttgtcc gctatggaac gggccggggc cgaggatatg ccggaggatg
      181 ccgagcgcaa gggattaggc acaccggcca cccgcgccgc cattctggaa aagctggtgc
      241 agatgggctt tgtccagaga aaaggcaagc agctcattcc caccaaggac ggcatcaatc
      301 tggctgtggt gctgccggag gcgcttactt ctccccagct tactgccgaa tgggaaaacc
      361 ggctgaccga gatcgccaag gggcaggccg atcctgacga atttatggac ggcatcgaga
      421 cgcaggcgcg ggagctggtg cagacctatt cctgtatcag tgaggaaaag cagaaattgt
      481 ttcaggccga gcgtgttcct atcgggacgt gtccccgctg tggtgaggct gtctatgagg
      541 gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt cgtcatgtgg aagaatgacc
      601 gattttttga ggaacgcaag aaagcgttca ccccgaagat cgccgctgcg ctgctcaaaa
      661 ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac cggcaaaacc tacgacggga
      721 cggtgctttt ggctgatacc gggggcaagt acgtcaacta ccgcattgaa cacaggagct
      781 aaaggacagt aacacagcaa gcatagggag tgtggctaca ctcccggtaa aatcccccgg
      841 ccgcgtccct gccgggccgg ggatttttgc ttgttgggga agtccccaac cccctatttt
      901 ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag tattttctat ccaagtccat
      961 aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc tggatttgcc caccacaacc
     1021 gaaacgctgc aagccgccct gcgggatgtt ggcattactg ccgacaatcc gcaggatttt
     1081 tttatcaacg ggtattcctg cccggaggac agacaccttg ccattcccta tgacatggtg
     1141 ctggccgccg acgtggacga gctgaatttc cttgccgccc ggctgggcca gcttgacgcc
     1201 gccgagatcg cagagctgaa cgccgccctg caaaatcccc gtggcggctt tgagaatatc
     1261 ggccagatca tcgactacac cgaaaatgtg gattactatg tgcatcttcc cgacgttagg
     1321 ggggatggac agttgggcga ctactacctg aaccgttccg gcatggtgga tatgccggag
     1381 gaatggaaag ccgggattga tacggcgcag tttggacgcc acattgccca gcaggaacaa
     1441 ggcgttttta cccagtacgg ctatctggtg agaagcggcg acgaatggca gcgcgtccat
     1501 gagggccagc cggtgccgga ggaataccgg gtgatgcact acccccagcc ggaagctgac
     1561 cgggaggaac cgcaggccca gcagccggag gcatccgaaa gccccgacaa agcgccgccc
     1621 ccggttatcc cgatcatgct ggacggcatg aacagcaaag aacgcatgaa agtgattact
     1681 gaccgtctgg aaaccggcgt acaggagctt tttgaaagta gccgctatat ggcttactta
     1741 tccgctatgt ccaagatgca cgattacagt ctgaacaata cgctgctgat cgccatgcag
     1801 ggcggttcga ttgtggcggg ctataacagg tggcaggatg aatttgaccg ccatgtgaag
     1861 cggggcgaga aaggcattaa gatactggcc ccggccccgt tcaaggttaa gcagcagatg
     1921 gagaaaatcg acccggccac ccagcagccc gtgatcggcg cggacggcaa gcccgtgacc
     1981 gaagaacagg aagtgacggt tccggccttt aaggtggtga cagtctttga catcagccag
     2041 accgagggca aggattttcc catgccctat atcagtgaac ttaccggcga cgtgcagcag
     2101 tatcaggatt tctttgccgc gctggaacgg acatcaccct atcccataag cattgagccg
     2161 atttcagacg gggccaaggg ccgctgcttc tacgcaaaac agcgcattgc catcaacgag
     2221 ggcatgagcg aattgcagac cctaaagacc gccatccatg agattgccca tgccaagctc
     2281 cacgccattg accgggaggc ccccgccgag gaacaggcgg cccggccaga ccgcagcacc
     2341 cgcgaggtac aggccgaaag cgtggcctat accgtctgcc agtattacgg gctggacacg
     2401 tcggactatt cttttggcta tgtggccgat tggagcagcg gcagggagtt atccgagctg
     2461 aaagcctccc ttgaaaccat ccgggccacg gccagcgaat tgataaccga gatcgacggc
     2521 cattttgccc agcttcagca ggagcgcgag gcccggcagg aacaggaggc cgtcacggtt
     2581 gacgttaccc cggaggcagc ggccccggcg caggccgagg aagaacaggc cccggccctt
     2641 gatccggcag ccgagccgct tgtgactgtg ctatggagcg aaagccccca gctaaaggac
     2701 ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg cccgtctgga tgcagcatat
     2761 ccggcagagg caggctatga caaaaccgcg ttccgcattg actgtatctt tcagggcgag
     2821 ccggacagct acgaggggcg gcaggatttt ggagataggg acggttccct gatcgaacat
     2881 atccagagct accaccaatt ttacgcgcag gacgaaaact ggaaaaagca tgtaatcgct
     2941 acaaccggca tagagggctg ggagcaggac aaggcccatc gggatatgct gttaggcgag
     3001 tttgtcccct atatgaagct gcattgcagc cttgcccaaa tggaacagac ggcgcagggc
     3061 cgcttacagt ccggggaaac cctgacgccg gaggaaacgg cctatttggg ggcgcttctc
     3121 tcttatgtgc aggagtgccg ccccatgctc aatcagggcc agtatcagtt gccggagccg
     3181 ccgaaactgg ctgacttcga ccagagcttg caggactaca aagcacaggt acaggccgag
     3241 atcgcgcagg aggccgccgc agccggtatg accgtggagg aatacgcggc ggctggctat
     3301 gaagcacccc aacaggacac cttttccatc taccagcttc gaggcgggga ggaaacccgc
     3361 gactatcggt ttgagccata cgagcgttta caggccgcag ggctggccgt ggacagtgcc
     3421 aactatgacc ttgtttatac ggcaccgctg gataaaagca cgtctttgga ggatatttac
     3481 cgcacattca acatcgacca cccggcagat tttaggggac acagcctttc cgtttccgac
     3541 gtggtggtgc tgcaccagaa tggacaggac accgcccact atgtagacag cttcggctat
     3601 atggaagtgc cggagttttt gcgggaaaat cccctgcgta ccgccgaact gtctacggag
     3661 cagaacgaaa acatgattga cggcattatc aacaatacgc cgcccgaacc gcagccggag
     3721 gaacccgaac agctatcttt tgaggatgtg gcagttgctt ccggccagac acaggaggaa
     3781 cccccggagg ctgcgccccc gcaaacggct gtccgctatt atcccatcaa tgagggagcc
     3841 gcccgccgcg ccaaggaagc catgagcttt tctgactacc ggcccggcag cgccacggcg
     3901 gaataccggc actatgtcga tcaggccgtg gaaattgcgg agcgccagaa aaagcgtgtg
     3961 gagccagagt atcacgaaaa gatagacagc ctccttgaca cctacgcccg caagctggcc
     4021 gagaacatga ataagggcta tgagattacc gcccgcgtcc cctctgtcct gattgccggg
     4081 ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg ccgccagtga ccgcaacatg
     4141 gaggaatttc agtacattca gggcttgctt gacaaaatcc gcagcaccgg catgggtggt
     4201 atcagcgccg acgatccgca ggccgtttcc aagctggaaa agaaactgga aaagctggaa
     4261 gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc gcaagcatgg gacgctggac
     4321 ggatgccccc atctgaccga aagaggcatt gaaaacctga aagcggatat ggcgtcgggc
     4381 tggcactatg agaaaaaacc gttccagtcc tggcagctct ccaacaataa cgccgagata
     4441 cgccggttaa agggccgcat tgaggaattg acccgccaaa aggaggcggc ctatgtggga
     4501 tgggagtttg acggcggcac tgtggagatc aaccgggtgg ccaaccgcct gcaaatcttc
     4561 tttgagggga agccggatgc tgctgtacgc gacgagctga aaagcaacgg tttccgctgg
     4621 tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca ccacaatccg cgtcgctgac
     4681 cgtatcaagt gcatccagcc cctttccggc gaaaagccca gcgccttaca gcttgcggcc
     4741 cgcagggaga aagaaaagcc ctctatccgg gcgcagctca acgcggccaa gaccgaacag
     4801 gccaagcagc caccggcgcg ggaaaaatca aagggattgg aggtagagta aatgcagaag
     4861 tttgaaaatg tggacgttct ggccgcgctg gaacagatca tgcggcagaa caccgcgttt
     4921 tatcaaagtg actttggcat tgacaaagac attatcaggc gggatgccgc cagcgatcag
     4981 gccgcagaca agaccctgct ctggatgtcc cggccctccg ggacgtactg tttccgggaa
     5041 cgtgatgttt tcctgaaaga cacccgacag tacaacacat ggaaatttta cggggagcag
     5101 acccgtgaca agatactggc ctacgcggtg gagctgaccg gcacacaggg cggcactatc
     5161 cggggcaatc tctatgagct ggactatccg cagcatttcc ggcacgtcat tgaaaccgcc
     5221 cagccggtga gtgtgaaccg gctcttttat gagcatggca cccgtgacat cccggagagc
     5281 cagcgttttg acggaagccc tgaccgggta ttgggaaact ttttgtacta tgaggcccag
     5341 ccccatgacc cggctgtgct gcaggaggcg ctacggacgg agcagcacag ccgggggcgg
     5401 gccgtccccg gcgatttcaa agtccacgtc accgctttgc atgacagccg gattgaaacc
     5461 gaggcccggc gtattgtgga cagcctgaaa gatctgtccg cgcctaacag ccccaataaa
     5521 acgcatttca tggtggagat ctccccgcac tttgcggcga ttgcttccac caaggataat
     5581 gaccggctct ttgccatgct gccctataaa tccctgtgtt ttaccgggat gaaaggcagg
     5641 catggcgtgt atgccgtgat taaccgggac gagcgccggg atgtgagtat tcgcaggccc
     5701 cggccctcta tccgcaagca gctatccgac agcaaacagg ccgccagccc aaaaaaggcg
     5761 gcggccagaa ccaaaaagaa tgaattggag gtataaggaa tgatgcactt taccgtggag
     5821 gaagaaaacc tgatctgtat gtaccacaat gcagaccggc gcagaacgat aaacaggctc
     5881 accgccgtca tgctggttat ggatggggat atgcgggcgc aggcccagca gaccctttcc
     5941 aagctggaac gcatgacgga tgccgactat gacggccaga agttccactt cacagacgaa
     6001 taacgaacag cagacgccgg gcgcggcggc catgtggtcg cttgcgtccg gcgtcttttc
     6061 tttttgccta ttcctacgca tttagaaccc ggtattgtcg gggataggat gaagtattcg
     6121 cttgcccctg caaacatgcg ggagaagcct tgcagcgggc ggttttcaat cccttaaatg
     6181 cgtaggttgc agctataatt agacccctga aacagatata gccgcgcagc gggtgtatct
     6241 gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa gagggtgcag ccctcttgcc
     6301 gtctatctct gttccagcgg gaagctcggc aatgcttcca gcagtttcag ggaaatgcgc
     6361 tgccgtatat cttcgtcaat gactttcttc tttttgccgt ttgcctgtct gatctccacc
     6421 gtggaaagct cgttgatgta gtcagcataa tgggccaaca ctttttccgt ggcccacctc
     6481 tcaccggcaa cggcggcttt gatcgtttca tagtccagca gcttttcttg tccgtccatc
     6541 ctgatacccc cgtcagactg attttttcta tgataccata ttcggaagca tggaaccaga
     6601 gaaaacggcg cttttctgct ggtaaattct acgcatttag aacagcactt cggcggggat
     6661 aggataaaac acccacatac cccgctgacc atgccggaaa agccttgaaa tataagggct
     6721 tttacagctc taagtgcgta ggtttccagt atcttttccg ctggcgctcg gcctctttgc
     6781 ggcggcgcat ccgtttggag caggccgcgc agtatttggc ccggttagac ttcggcgtga
     6841 acgtggcccc gcactcacag cagcgtttca cttcgtcccg gtctttggtg atctcggcat
     6901 acagggccgc atccagcggg aggacagcgg ccttaaacca gcggcacaga agcgaatagg
     6961 aaatgctctg tacgcaaata cattcttcgc cgtcgtccag caacaggcag ttgccgtcac
     7021 aatagttgca gcactcatgc gtcagcttcc gggccttgcg atattgaggg taattcattc
     7081 gtggaatact catagctctt gcgtccattc ctttcccggc gtcatacgca aaatgccgtc
     7141 cacattctgc ttgatgatgc cgtattcctg catctgcttt ttcgcttcgc cgtacttctc
     7201 atacagccgt tccttttcct cggccaatcg cttgtactcg gctttcaggg cgtacagatc
     7261 ggggagcttt ttcacaccct gtccctttag ggcctttgcc gcagcttcat acaaaatcaa
     7321 cgtgctttca tgctcacgcc ggaattttgc cttatccgct gcattgcggt attccatagc
     7381 cacgggccag agctgcttgt aggtagaaat attctttatc agcaccgcca tatccgcaag
     7441 ccggtgttcc atagctttga gggtagcggc ggcctcgtcg ctggcggcgg tgatctcggc
     7501 cactttgctt tccaaatccg ggtaggtgtc tatcccatgt tcagtcagga aattcaaagt
     7561 ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc tcatagcctg ccgactgctg
     7621 ggccttaatg ttgttttcca gatcaatccg caaagaaatc cccttgcgct ctttgggcgc
     7681 tctggtgata acgcggcctt tgatacgctc tttgatggct tcctcggtgt aggcttcccc
     7741 caatgtcttg cagcgggtaa agcgttcctg tccgggagcg cggaaagaaa taaactttcc
     7801 cggcttgatc tcatagcctt gcgcctgcat gagccgcagg aaatcatcaa aatcttttgc
     7861 ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct ttccagctcg tccctttccg
     7921 ctgggcgtcc cactcggcat agctcttgcc cttactctgg ccgggcttca ccacggacaa
     7981 tccgttttcc ttgcacagcc tgtcgctggt gcgccggata taggcatagc tctgtttgtt
     8041 ggaaatgtac ttgcgctgat ttaccatatc cacggcacag aaaataatgt gattgtggac
     8101 gtggccctta tcaatatggg tagtcagcac atagggatat ttcccttgca gcacttcctc
     8161 ggcaagctgc ttcccgatct catgggcctg ttccggcgtg gcttcgccgg gggcaaagga
     8221 ttgtatcagg tggtggccca gattattgcc cttttggtat gcctgcgcca gcagcatttc
     8281 aaattcaatg tccgctgttt cataagagca ggcaaaggag gccaccagca gcttgccgtc
     8341 agttttgtcc gggttctcga tgtagtcaag ggcttttttc agcgttgact tgataggatg
     8401 tatctttgta actgccatat ctgcgccagc gcccccttta tatcctcgat gtcctgtgcg
     8461 tacaccggcc ccataccgtt cagcctcttt gcaatctgat tgacgttgac gccgatcttc
     8521 tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca tgttgacgat atagccgtca
     8581 atgagcatct tccgggcgta ggctgaaaag ttcttcgtat gcagcagcgc cattttctgt
     8641 tcaatcatag cccgttcctc cggcgtcaca gggacgcgca gcacgatttt tcgctttctg
     8701 tttgccatgc ttcaccgtcc tttccattca ggggtttggg gatttcccca acaagcaaaa
     8761 cggacgcggc ccggcaggga cggggcaggc cgtttttccg ggagtggcta cactccctat
     8821 gcttgctacg ttactgtttc tccactaaca agacgtttcc aaagccccaa actacccgca
     8881 aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg
     8941 ctaaaagtaa aacagaggat gccagttaca catcctccgt ttcattcttc ttcggctttt
     9001 caattagtat ggcgctgtca atcgcgccct cgatgatcgg caggtattct tcggggcaga
     9061 gtttcagctt gtggctgacc ctttgccgca gttcgctttc ctcccgcatg atctccgggt
     9121 taaagtagcg ttccacagga agcccgcagg ctttaataag ctgtatgaca accggcagac
     9181 tgggaatggc cccgtcgttt tcgatgttgg caagatagcg ccagtcaata tttattttct
     9241 ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc cgctttcaca tccgcgccga
     9301 aagtttcaaa gccgggacaa tcttcaatat tcgccatatc gcacctccca cttacattgt
     9361 atatttcata ctctgcccgt ggaatgttgg tatatgcacc tttcttaaag tttatatttc
     9421 acattttgtt tcttttattc ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac
     9481 tatggactac atgacgttga aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat
     9541 aaactactat tgtgctgcgg gacgcatccc cggcgctgta aagatggcga cgatctggct
     9601 cattcccaaa actgcggaaa agccgattga caggcgtaca aagaaaggaa aggcggttac
     9661 tgaatagtat aatgaaaccg gcgcattttg gatatggttt tgaagagaga gaaagcatgg
     9721 tattttgtca cggaaaaact ataagatgat agatggcagg ctgctccaga ccgataaaaa
     9781 gtattctcat ctgaaacaga aagaaaagat ttacacatgg atgtatgaag aagcgaaact
     9841 ttatcatgac agaaatggaa aatgcccgaa acaaagacga ggaagctgct gctgtatcag
     9901 ccgtatatgg tagaattgaa aaggccggca tctggattct ttatggtgaa gtctccagac
     9961 attggataac tgctggtcta atgcgccttt gagtatgggg tgattgattt taataccggg
    10021 gaggtacggc tttttgagga aaatattctg ggatttacat ttggcgatta ttacatttcc
    10081 tgtaactgca aggacacgga tgatgaagat atgaggaata tagacttgct gattttctac
    10141 tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg
    10201 gagtacacac aggccctgcc tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag
    10261 aggccgcgca ttttgctgag gcttggggtg tccccaacaa gcaaaacgag aacgccgcag
    10321 gcgggcaagt ggtggatcag aatatccgta aaggatattc tgatccattg cttgccagta
    10381 ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta
    10441 attattatta tcttgattta attcgtttaa accaataata tagatacata caccatgaaa
    10501 agaagcgagt atcccaatga cggatgatta aaatccgaaa agaaatcgct ttgaaactac
    10561 agggaaacta cagactgtta tgttatcttc ttaaaatgga gggattttta tgtcgatacg
    10621 aattctactt gtcgaggatg atgatcatat ctgtaataca gtaagggcgt ttctggctga
    10681 ggcaggatat caggtggatg cctgcacaga tggaaatgag gcatacacca agttttacga
    10741 aaacacttat caactggtta ttcttgatat tatgctgccc ggtatgaacg ggcatgaact
    10801 tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg atgatgacag ccctgtcgga
    10861 tgacgaaaac caaatccggg cgtttgatgc agaggcagac gactatgtaa caaagccatt
    10921 caagatgcag attttactaa agcgggtgga agccctgctg cggcgcagcg gtgcgctggc
    10981 aaaggaaatc cgtgtcggca ggctgacact tctgccggag gatttcacgg tactttgtga
    11041 cggtacagag ctgcccctga cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa
    11101 caaaggcaga accttaacgc atgaaatcat tttgtcacgt atatggggat atgactttga
    11161 gggtgatggc agcacagtcc acactcatat caaaaatttg cgggccaagc tgccggaaaa
    11221 tatcatcaaa accattcgcg gtgtaggata ccgattggag gaatcataat aatggaaaga
    11281 aaagggattt tcattaaggt tttttcctat acgatcattg ccctgctact gcttgtcggt
    11341 gtaacagcaa cactgtttgc acagcaattt gtgtcttatt tcagagtgat ggaattacag
    11401 caaacagtaa aatcctatca gccattggtg gaactgattc agaatagcga taggcttgat
    11461 attcaagagg tggcagggct gtttcactac aataaccaat cctttgagtt ttatattgaa
    11521 gataaagagg gaagcgtact ctatgccaca ccaaatgcca atacatcaaa tagttttaga
    11581 cccgactttc tttatgtggt acatagagat gataatattt cgattgttgc tcaaagcaag
    11641 gcaggtgtgg gattgcttta tcaagggctg acaattcggg gaattgttat gattgcgata
    11701 atggttgtat tcagcctttt atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc
    11761 aaagccttag cggacagtgc gaataaaatg gcaaacctga aagatgtacc gccgccgctg
    11821 gagcgaaagg atgagcttgg cgcactggct catgatatgc attccatgta tgtcaggctg
    11881 aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca
    11941 cagcgatatt tctttgcggc agcttctcat gagctaaaaa cgcccatcgc ggctacaagc
    12001 gttctgttgg agggaatgct tgaaaatatc ggtgactaca aagatcattc taagtatctg
    12061 cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca tttccgaaat actggagctt
    12121 gtcagcctga atgatgggag aatcgtaccc atagctgaac cgttggacat agggcgcacg
    12181 gttgccgagt tgctgcccga ttttcaaacc ttggcagagg caaacaacca gcggttcgtc
    12241 acagatattc cagccgggca aattgtcctg tccgatccga ggctgctcca aaaggcacta
    12301 tccaatgtca tattgaatgc agttcagaac acgccgcagg gaggcgaggt acggatatgg
    12361 agtgagcctg gtgctgaaaa atgccgcctt tttgttttga acatgggcgt tcacattgat
    12421 gatactgcgc ttccaaggct gttcacccca ttctatcgca ttgatcaggc gcaaagcaga
    12481 aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa aaacgctgga tgccatgagc
    12541 ctccagtatg cgctggaaaa cacctcggat ggcgttttgt tctggctgga tttaccgctc
    12601 acatcaacat tgtaaatatt taaaacttaa atgattttga ccgacaggta taaccctgcc
    12661 ggtctttttt tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc
    12721 ttgccataat aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg
    12781 atcaaatgga aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac
    12841 aatccgtgga aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc
    12901 tgtttttggg gcggagactg gcttccgtat tggaggcaac acagataccg cccatccctg
    12961 caactcatac aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca
    13021 ccgccaaaga acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc
    13081 ccatccccgc acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata
    13141 ttcggatttc tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc
    13201 cgattgtcgc atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa
    13261 ttgccgaata taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt
    13321 gggtggccgt gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg
    13381 acggaattca ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt
    13441 ttggttttat tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc
    13501 cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt
    13561 gccttgagga atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa
    13621 aagattgaca cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg
    13681 cttttatgcg ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc
    13741 gctttcccat ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga
    13801 catgatatac caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg
    13861 cttattgata agaccggggg aacccttttc tttctggtgg ctggtacgcc atgcggacaa
    13921 agataccccc tataaagacg gccttacggt gaccaatggt aaactcacca ccatgtcggg
    13981 cggcggtatg tgccagatga gcaatttact attttggatg ttcctgcata cgccattgac
    14041 aattatccag cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat
    14101 taaaggggtg gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac
    14161 cgactgcacc taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtc
    14221 cgccgacaaa gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg
    14281 tgaaagtggc gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac
    14341 cggggaaata atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct
    14401 cccggaaagt gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt
    14461 tttggatgcg aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt
    14521 atccctacgc tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat
    14581 caatgcgtta gcgtaggcca taagtccgag gtttccgagg cgacaattct tgcgctgaga
    14641 aaggtcgggg taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact
    14701 gccgccgaga gaatgggaat ctcggttggc acggttgcgt attcgccgga cagcgttgcg
    14761 gattatgctt tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc
    14821 gtggcgcaac aagatttcag attggatcgt atccggggga aagaactggg ggatatgact
    14881 gtgggagtta ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt
    14941 ggatgccatg tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt
    15001 gatgagcttc taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc
    15061 cgccatctga tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac
    15121 accgggcgcg gagcgcttgt cgataccggg gcgctggtgg aggcgttgga aagcggaaag
    15181 ctgggtggtg cggcactgga tgtgttggag ggcgaggatc agtttgttta taccgattgc
    15241 tcgcagaaag tgcttgacca tccctttttg tcgcagcttt taaggatgcc gaatgtgatc
    15301 atcacacccc atacggcgta ctacactgag cgtgtgctgc aggatactac agaaaaaaca
    15361 atcaggaatt gtctcaattt tgaaaggagt ttacagcatg aatagaataa aagtcgcaat
    15421 catcttcggc ggttgctcgg aggaacatga tgtgtcggta aaatccgcaa tagaaattgc
    15481 tgcgaacatt gatacggaaa aattcgatcc gcactacatc ggaattacaa aaaacggtgt
    15541 atggaagcta tgcaagaagc catgtacgga atgggaagcc gacagtctcc ccgccatact
    15601 ctccccggat aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg
    15661 gcgtattgat gtggctttcc cggttttgca tggcaaatgc ggggaggatg gtgcgataca
    15721 ggggctgttt gtattgtctg gtatccccta tgtgggctgt gatattcaaa gctccgcagc
    15781 ttgcatggac aaatcactgg cctacattct tacaaaaaat gcgggcatcg ccgttcccga
    15841 atttcaaatg attgataaag gtgacaagcc ggaggcgggt gcgcttacct accctgtctt
    15901 tgtgaagccg gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga
    15961 acttaacgct gcgatagaag cggcaggaca atatgatgga aaaatcttaa ttgagcaagc
    16021 gatttcgggc tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt tgattgtcgg
    16081 cgaagtggat caaatccggc tgagccacgg taccttccgc atccatcagg aaaacgagcc
    16141 ggaaaaaggc tcagaaaatg cgatgattac agttcccgca gacattccgg tcgaggaacg
    16201 aaatcgggtg caggaaacgg caaagaaagt atatcgggtg cttggatgca gagggcttgc
    16261 ccgtgttgat ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg tcaataccct
    16321 gcccggtttt acatcgtaca gccgctaccc acgtatgatg gccgccgcag gaatcacgct
    16381 tcctgcactg attgacagcc tgattacatt ggcgttaaag aggtgataag tatggaaaat
    16441 ggttttttgt ttttagatga aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca
    16501 tgggataact tcacaggaaa accggtggat gggtatgagg tgaatcgcat catcggcaca
    16561 aaggccgtgg cgtttgctct gcgcgaggca caaatccatg cggctgcgct tggctatggc
    16621 ttgcttttat gggatggata tcggccaaga actgcggtgg actgcttcct gcgttgggca
    16681 gcgcaaccgg aggacaatct cacaaaagaa aaattttacc ccaatataga gcgagccgag
    16741 ttgattacaa agggttatgt ggcctcacaa tccagccata gccgtggaag cgcaattgat
    16801 cttacgctct accacctgga tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt
    16861 atggacgaac ggtcgcatca tacagcaaaa gggatagggg atgcagaggc acaaaatcga
    16921 agatgcttgc gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg
    16981 cactataagt tgattgatga gccatacccc gatacctatt ttaattttgc cgtttcataa
    17041 tgaaagtatt ggttttgtat cagattttgg atttgataaa atctatgctg gtcatggaca
    17101 atcttttact ggaaagtggt taaagcatct tttgtaatac attgcaaatt atagtattca
    17161 atctgcccag cggcagaaaa gaaaaaaacc gctgttgggc agacctttcc cacgcccatt
    17221 ttgtattttg ctggcggcgg ttttgagaaa tcaaggccgc cgttcttttc tggcagcaaa
    17281 aggtgtggcg gcccatagga ggatgccgct atgctgtttt gctattgcca tgctctaatc
    17341 cacttccacc actcaaaaaa agccttgccc ctccatgaac tgaaccccaa aagttgaact
    17401 atttaatgga ctgt
//
LOCUS       DAZBWR010000309        16775 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.058, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000309 DAZBWR010000000
VERSION     DAZBWR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.058"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            366..1229
                     /locus_tag="ACHGT5_003045"
     CDS             366..1229
                     /locus_tag="ACHGT5_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN7218929.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1329..1811
                     /locus_tag="ACHGT5_003046"
     CDS             1329..1811
                     /locus_tag="ACHGT5_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN7218930.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2074..2541)
                     /locus_tag="ACHGT5_003047"
     CDS             complement(2074..2541)
                     /locus_tag="ACHGT5_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN7218931.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2664..3515
                     /locus_tag="ACHGT5_003048"
     CDS             2664..3515
                     /locus_tag="ACHGT5_003048"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN7218932.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3766..5724)
                     /gene="nagE"
                     /locus_tag="ACHGT5_003049"
     CDS             complement(3766..5724)
                     /gene="nagE"
                     /locus_tag="ACHGT5_003049"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN7218933.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5721..6593)
                     /locus_tag="ACHGT5_003050"
     CDS             complement(5721..6593)
                     /locus_tag="ACHGT5_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN7218934.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(6935..7807)
                     /locus_tag="ACHGT5_003051"
     CDS             complement(6935..7807)
                     /locus_tag="ACHGT5_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN7218935.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            7935..8504
                     /locus_tag="ACHGT5_003052"
     CDS             7935..8504
                     /locus_tag="ACHGT5_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN7218936.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8571..9308
                     /locus_tag="ACHGT5_003053"
     CDS             8571..9308
                     /locus_tag="ACHGT5_003053"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN7218937.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9539..10807
                     /locus_tag="ACHGT5_003054"
     CDS             9539..10807
                     /locus_tag="ACHGT5_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN7218938.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11294..11758
                     /locus_tag="ACHGT5_003055"
     CDS             11294..11758
                     /locus_tag="ACHGT5_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN7218939.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11742..12371
                     /locus_tag="ACHGT5_003056"
     CDS             11742..12371
                     /locus_tag="ACHGT5_003056"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN7218940.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12725..14203
                     /gene="msr(C)"
                     /locus_tag="ACHGT5_003057"
     CDS             12725..14203
                     /gene="msr(C)"
                     /locus_tag="ACHGT5_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN7218941.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14250..14696
                     /locus_tag="ACHGT5_003058"
     CDS             14250..14696
                     /locus_tag="ACHGT5_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN7218942.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14895..15410)
                     /locus_tag="ACHGT5_003059"
     CDS             complement(14895..15410)
                     /locus_tag="ACHGT5_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN7218943.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15527..16729
                     /locus_tag="ACHGT5_003060"
     CDS             15527..16729
                     /locus_tag="ACHGT5_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN7218944.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         5300 a         2988 c         3483 g         5004 t
ORIGIN      
        1 gatgaggttg aacttttaca caagattgtg gacttgactg caaagaaaaa tccaagcagc
       61 gttctgatca gcagtttgaa caacaatttg ctttgtacag taggtagtaa gcggattaac
      121 taagggaata aaagatattt ttaccgcttt gtttacttag aatctgtctt ccccaaaatt
      181 tatttttcta aaggtaaaac atagataggc agataatacc aattatccgt tacgaaacgc
      241 tgcttgagaa ttttctttag atccatacaa cattttctac atctgactaa attggttcct
      301 gattcatcaa gtaattgact ttctttgata atatgataaa aaggcaatca aggaggaaga
      361 taaatatgct taaagatctg aaccaattga tcgattacat cgaagaacat ttgacagaag
      421 gactttccta taaaataata gccgaaaata caggaatctc tgaatatcaa ttaaaacgca
      481 tctttacgtt tattgcaggg atgtcgctaa tggactatat caaaaaccgt agattggctt
      541 tggctaatcg cgatcttcta gatggatcaa gagtgacaga aacggcgttt agatatggat
      601 atgaaacagt cgaaggtttt tcaagagctt ttcgtgattg gtcaggatat ctgccttcag
      661 aaatcagcag atacggcata caaaaatctt ttccacgctt gacatttttt attgatgtca
      721 gaggaggaaa atcgatggaa tttcgtatcg aaaagaaaga acagttttat gtggcaggtg
      781 tggcgaaaaa agttcctgtc aaatttgaag gagtcaatca agaaatacaa aagttagcgc
      841 agacgatcac ggagactcaa agggaagaga tgcataagtt aggagattta tatccaaatc
      901 aagtaatcaa cgtttcttat ggatttgatg gagaccgctt ggaagaaaag ggcagtttga
      961 tgcacatgat tggctttgct acgacaaaag aaaatccgta cgctgattta gaaatgcttg
     1021 ctatagaaga acacacttgg gccatttttc ctaataaagg gccatttccc caaactttac
     1081 aagatacttg ggggaaaatc tatgcagaat ggcttccttc ttcagattat gggttagtcg
     1141 aagctcccga gatctctttt acaaaatggg gagaggattt cagtaatgtt tacagtgaga
     1201 tttggattgc agtgaaagca aaaaaatagt taaaaaataa aaatatccct agaaaaaaga
     1261 gcgtaatcgt gtcatttccc tttaatttta tgtaagatga acttaatata atagaaaggg
     1321 tgggagaaat ggcaaagtat aggatcgaag aaaaaccaga ttttacggtg acgggtattg
     1381 gtgtggaatt gaagagtagc aatacggatt ttgtcggttt ggctgcagag aaacaagcat
     1441 ttgagcaaaa agttatcggt aatggaacaa tagatgcatt gaaacaagct gctgaaaatc
     1501 attatttctt tacagtcaac gaaccctata acgggaaaat gatgtactat gtcggtgttc
     1561 aaacgagtca ggatgttcca aacactacgc gaatcatccc attcccaaaa ggaacgtatc
     1621 ttgttgtaag cgctttagca gaatcgcctg aagaattaag aaatgatttg acgaatacgg
     1681 cctttgggca tgttttaaga gaagtagaag attacgccta tgttgggggt ccaaatgctg
     1741 tagtcgaaat gggagttacc aacgggaaat atatgggaga aatctggata ccggtagtca
     1801 ataaaccatg ataatataga ggtcgtgaaa gtagcgcttt gacctgagca ttagagagga
     1861 aaaatgggaa atagctttcc atattttgca tttttcagaa ctaatgccga gggtcagcta
     1921 cttgaacacc gtttatcaag acgtcatgac ttcagcgctt tgacctgagc attagagagg
     1981 aaaaaggggc atgacaaggc ttttgtcccc cggagaataa aagaatttat gtagacgatt
     2041 ccgatcagaa aaatgcaggc acagtagaga gaattataaa aatctctcta cgaggcctgt
     2101 gttttctggt cttttttctt tttccaaaac ggctatttta tgtatcagac tatcaagtgt
     2161 gatcttagcc atttcttctt ctgccttttt ctgcagccga gcgtagcttt cttctaaagc
     2221 ttcttggata ttcgcaccaa cgacgcaatt tggatttgtt tttggatcga cttggataag
     2281 ctgtgtatta ccttcgatgc tgcgatagac atctagtaaa gaaatctgat ctggactttt
     2341 ggcaagggaa ggctgcggtt ttccggtttt tgtaaagatc aaaccggatt ttttgagctg
     2401 actcatgatt ttacgtacat ttgctgcatt tgtttcgata ctactggcaa tcatctcact
     2461 agaaagtgat cgggtatctt gaaacatttc aatataggca agtacatgga ttgcatcact
     2521 aaattgcaga gaatacttca tcagttgtca tcctttcatt ttttttgctt gacttatctt
     2581 accacactct ttatgataaa ggtgtaattt ataaatgtac agctgtaaat cttaacaaat
     2641 aaaaagaata gggagtggca aatatgcatt atttagtaac aggagcaact ggaggttttg
     2701 gtggttatgc gctggaattc ttaaaaaaga tggttccaca aaatgaaatc tatgtactag
     2761 tacgtagtga agaaaaagga gaagcactga aagaagctgg atttaatata cgtattggtg
     2821 attatgatga tttagactca atgaaagaag cgttgaaagg aatagatcga ttgctttttg
     2881 tctctggcgt tccaggaaat cgtcaagccg aacataagaa tgtagtgaag gcagcacaag
     2941 cagctggggt atcttatatc gcttatacaa gttttgcaga tgccgatcac tcgatcagtg
     3001 tattagcgcc agatcaccaa ttcactgaaa aaatgattaa ggattcaggt atttcccata
     3061 catttttacg gaataattgg tatgtagaga atgaattacc tatcattggg caggctttaa
     3121 aaactggtca attcgtttat gctgcaggaa atggaaaaac aggttgggca ttgaaacgtg
     3181 aatatgcaga agctgcagca aaagtactag tagaaagtga ctctccagaa atcttagaat
     3241 tatccggcaa tctaactgac tatgaagaat tagccaaggc attagagcgt gcgacaggta
     3301 aagagttaga aattattgaa gcaagtgatg cagcgtttgt cgaaaatcta aaagaagccg
     3361 gattccctca agaagcagct gacatgtttt tagcgatcca acatgatatt aaaaaggatc
     3421 agttagctat ttcctctgat gatttagaaa aagtattggg aaaaccatta acttctcttg
     3481 aagcagcatt aaaagaaata ttggatcagg aataatgtat aaagaggttg tgaaaaagcc
     3541 gattagtttt gagtatgaag aaaaattttg tgaaaaagct gttctgcatc aagtaataag
     3601 aagagtcaaa taaaaaatag ctcttcatat gtttcgttta gctggactta ttaccgcgga
     3661 gttggcagga gaacgccgtt tttattcgga aaaaggagct gagacaagat ttttgtctca
     3721 gctcctgttt gtttatcgat aaaaaagtct ggggaagacg atggctcagg ctttgttgga
     3781 aacagagccg attttatcat ttgcttttac atcacgtgcg ggttcgattt ctaagctttg
     3841 gactttgtcg ccatttgtga atacaacgac catatccgtg tttttgcctg cagattgaat
     3901 agcggcaaga tcaactaaag caatcaattg acctcttgcg actttttgac cttcttctac
     3961 atataaagtg aatggttcgc cttttaagtc aacagtgttg atgcccatat gaagcaagac
     4021 ttcgatacca gcatctgttt ggattccgac agcatgtttc gttgggaaaa tgtttgtgat
     4081 cgtaccagcg attggagaga agatttctcc attttctggt aatacagcat agccgtcacc
     4141 catcattttt tccgcgaata catcatctga aacttcagtg attggaatca gtttcccatt
     4201 tgcgatagcg taaatatctt gttcgatcgt ttcaccggca aatgtttctg ttttaaccac
     4261 ggtgtttgtt tgtgcaggtc gtgctgcagc accaccatgt agttttgcca tctcatctgc
     4321 aacgaattgt acttctgtac ctacgatgac ttgcgcattt tttccgtcga tgatcttcat
     4381 accaggtacg ccagtggctt tgattttatt ttgatcgatc aggcctgtat ctttgacttg
     4441 caaacgaagg cgtgtagtac agttatcgat cgaagttaca ttgtcggctc cgcctaatgc
     4501 agcgtagatt ttgcttgctt gtgccgcata tttattgtct gaattagtcg ttgcagtatc
     4561 gtcatctgaa agatctgctt cctcgcggcc aggagtcatc agatggaatt ttttgatagc
     4621 aaagttaaag ccaaagtagt aaatcactgc tgttactaat ccttggacga tcaacatcaa
     4681 tggctgattc gcaatcggat ttttcaaaga taggaagtaa tcgacaaatc cggcactaaa
     4741 ggcaaatcct gctgtccaat ggaagaatgc actcactgct aaagaaatcc cagtgaaaat
     4801 tgcatgcaag acatataatg gccaagcaac gaacataaat gaaaattcaa gtggttctgt
     4861 cacaccagta aagaatgaag caaaagctgc tgctagcatc aaggaagctg tttttgcttt
     4921 gttttctgga cgtgcatttc tgtaaatagc atatgctcct gctggaagcc cgaacatcat
     4981 gattgggaaa aatccagctt gatacattcc tgttacgcct tttgttcctt cagaagccaa
     5041 aaatttacca atatcattga ttcctgctac gtcaaaccag aacacggagt tcattgcttg
     5101 atgcaatcct gtagggatca gtaaacggtt gaagaagcca taaagacctg caccacccca
     5161 gcctaaactc aaaatgaatt tagcaaaaga aactaaagca gagtaaaccg gaggccatac
     5221 gagcagtagc actgcactga tcagcaacat gacaaaggca gccaagattg gcactagacg
     5281 cttgccgcta aagaaagata aagccatcgg taattttgct tcgtggaaac gattataaag
     5341 agctgccgca atcaatccgg caataatacc aataaatacg ttactgatct gagcaaaaga
     5401 tggatcaact gattctggtt tgatattcaa taaagtagct actgaatcag tttttaaaac
     5461 atttactgga acttcaaatg cgaccaaacc tgccaaggca gcagcacctg atttgtccac
     5521 tgacatccct agtgctaacc caacggcaaa cagcaaaggt aattgaccta aaactgcgtt
     5581 accgccagct tgtaaaaaat gagcgacaat gtcgttacta taagatgccc accagtttcc
     5641 gatccctact agtaaagcag cagcaggtaa gaccgcaact ggaagttgaa gggaacgtcc
     5701 cattcgttgt agatatgctt tcaatttttt tcctcctcat gattgttaac atttgtttgt
     5761 acgattgcgc ttcttaaacg ctcgatatgg atggccaagt aacccagttc ttctttcgtg
     5821 atcgcgagct ggtattcttt ggtcagcaac acacggattt ttttagaaat cttatagctt
     5881 tctgggtatt ttcgtttgac catttctacg atttccgtgt ctaaaacagc atattgttgc
     5941 gcatgggttc gttgtagaag cagtcgtaaa tgattcacca gtcttgaata attcagtgaa
     6001 aatggttctg aatggatatc tgtttccaat tctgattcca ctaaatggat gatgtcagat
     6061 acaatcgtca cttcacggat actgcgatgg ttattgttcc gaccgcttcg tccgctgtga
     6121 atatggattg cgatatatcc tgcttcgtca taagtatatg gaatcccaag ctcttttgtc
     6181 agatactcta ctgcccattg agcaatactg aattcatcac tgtaaagcac ttcgatttct
     6241 cttaaaagtt tgttgcggat gacgataccg ttattaatgt tctccgcaga aaaagcaatg
     6301 tggtcggtca aagcgatcaa tacatgttca ttcaactttt ccatcaaaac agtttccgca
     6361 tgatcaatga tcgtttctgc tgcaaataaa aagcgttcat caatctgatt aagtaaattt
     6421 tgcaacttga tttgaccttc tggttccatg acaaatatcc gttcgacatc acgatcaaaa
     6481 atcaaatcat ttctttttct gtcaaatcca atcccttttc caatagctac tttttcctgt
     6541 ccaccgtcat ctaccaatac tgcattttga ttaaagatct ttttgatttt catgcgcgca
     6601 ccctttttga agatttggtt ttcacgtttg ctagaccagt tgattgcggc caaacaaaaa
     6661 tagacacgaa aaacctccta gggaaaccat gttcctcaga gatttttcat gcctagtatg
     6721 atctagtcac atgaaacgaa ttattgttaa cgtaatcaat tttatataga cagttatgtt
     6781 ttgtcaattc tttttttata attatagtaa tttactggaa ttatcagaaa taaaaggaag
     6841 aaataaatta agaaacttat gttatttaat cgttggatct tttgcttatc ttattcgttg
     6901 ttaaaaagct gaaagcgaag gaaaaaaact ctacttaact gtatttctcc gttaggacat
     6961 ctaaaaattt acgagcagca ggagaaagag gctggtcttt tttccaaatg agattcagtg
     7021 aagcttgtaa tggcggatcg agtgggatga atattttttc cgtcccataa gtattgatga
     7081 tcccatctag agctaatgca ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca
     7141 ataagttata tgttccgacg atcgataact gatctatgct gaaacctagc cattcagcta
     7201 gttgactgtc cactgacgtt tgccgggaaa tgatcaaagg aacagttttc agttcctgcg
     7261 gatggatgac ggtttgcttt gctaaaggat gatttcgatg tatcaaaagt ccccaacgat
     7321 cttgggctgg cagtcgaaat gactggtatt tttgtttgtt tgtcggttcg attacgagtc
     7381 caaaatcaag taatccgcta tccagtttgt ctatgatatc atctgcattc ccgctatata
     7441 aatgaaactg gatatcagga tgcgccttga tcaacgaatg aaggctatca gaaatgaaag
     7501 agattgcttt agtttcgcct ccgccaatgt agatttctcc agagatgatt gcgtcatttg
     7561 ttaaattgtt cgccgttttt tctaccaacg acaagatttc ttttcctttt gaataaagat
     7621 atcgaccaga atccgtcaac tgtatctctc gggagccccg agtgaaaagt gtcacaccaa
     7681 gttcttcttc taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt
     7741 ttgctgctcg gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa
     7801 tatccatttt ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat
     7861 agttaagtat gttttataag tattagacat gataagccgt ccgttataaa gtaaggataa
     7921 aaggagggag caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag
     7981 taacggatcc gctctttgaa gtgatccatc agatccaagc tgaaaacgaa gagcctttag
     8041 cagcgctgaa tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca
     8101 ttagtgacaa agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca
     8161 aacatattag tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag
     8221 gtggcatctg tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga
     8281 accatctaac cgatccaaaa gaacgaagag gcttgttagt aaagccaatc cacatcaaga
     8341 aaaatgcttg gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg
     8401 ccattgttgc agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag
     8461 gaatccctgc caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata
     8521 agagatagtg agtataagca agagattttt caaaacagga ggagaataga atgaatgatt
     8581 tatcagaaaa agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga
     8641 agctattagc agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa
     8701 aagcaataaa agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag
     8761 attttgcaca agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg
     8821 tattgtacaa taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg
     8881 aatggcaaaa tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac
     8941 ttccaatcat ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac
     9001 atgttgttta tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca
     9061 tggaaggatt acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag
     9121 gagctgttca aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc
     9181 atgaagcgca aaaagaatgg ggattgacct ccgaagatat agcttctgct gtagcatttg
     9241 cgatagaaac gccagaccgg atgtctgtca gtaacatgat tatccgtccg acgacacagg
     9301 aagtataatc cataacggat aatatccaaa gagaattact tgttaaatag ttattcaaac
     9361 gaccatcaat ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgtag
     9421 aaaaagcaaa tttatcaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt
     9481 tactttttta gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat
     9541 gaaagcaaag aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac
     9601 aacagtgcta gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga
     9661 aacaggaatt gaaaacgata cagagagtac tttgcagtca gctactgtct cagaaaatac
     9721 aatttcaacc gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac
     9781 atcagcagaa gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga
     9841 tgggcaaaca ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg
     9901 ctatgctttt tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc
     9961 ctcaactgca caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca
    10021 agccatcgtt gaaagtggct gggggaacag tactttggct tctgccccta attacaatct
    10081 atttgggatt aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt
    10141 gaatgggcaa tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc
    10201 attgcaagat aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta
    10261 ttccggtgca tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg
    10321 tcgttatgcc actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa
    10381 tctgacacag tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg
    10441 tcatacagga gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt
    10501 aggctgggaa tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg
    10561 tttatacaat ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc
    10621 tttaacaaaa atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc
    10681 aatttatcga ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc
    10741 taaggaaaga gattggctaa caccgatggg ctggagatat gaaggaatag gattttatgg
    10801 ttattaaaaa gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa
    10861 aaacagattt tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa
    10921 caccgtttac tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta
    10981 agcatactga tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat
    11041 ttttggcggt gtaaaatttt ctactgcaga agagctatct tctatagttt gataagaatc
    11101 aataatcttg gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa
    11161 atttcttttc tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt
    11221 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
    11281 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
    11341 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
    11401 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
    11461 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
    11521 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
    11581 ccagaagaaa atcgagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
    11641 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
    11701 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
    11761 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
    11821 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
    11881 tgcaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
    11941 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
    12001 atatgcaatg ttacgaatat ggcgaagaaa tctataacca agcttacgaa tcagggaaga
    12061 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
    12121 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
    12181 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggagtc gaagcagatc
    12241 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
    12301 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
    12361 cgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
    12421 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
    12481 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
    12541 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
    12601 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
    12661 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
    12721 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
    12781 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
    12841 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
    12901 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
    12961 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
    13021 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
    13081 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
    13141 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
    13201 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
    13261 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
    13321 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
    13381 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
    13441 accggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
    13501 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
    13561 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
    13621 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
    13681 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
    13741 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
    13801 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
    13861 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
    13921 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
    13981 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
    14041 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
    14101 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
    14161 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
    14221 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
    14281 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
    14341 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
    14401 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
    14461 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
    14521 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
    14581 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
    14641 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
    14701 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
    14761 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
    14821 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
    14881 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
    14941 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
    15001 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
    15061 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
    15121 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
    15181 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
    15241 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
    15301 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
    15361 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
    15421 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
    15481 tttacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
    15541 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
    15601 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
    15661 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
    15721 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
    15781 tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg
    15841 attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc
    15901 tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa
    15961 agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg
    16021 tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt
    16081 ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt
    16141 aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat
    16201 cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat
    16261 tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat
    16321 tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt
    16381 agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc
    16441 agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact
    16501 gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta
    16561 tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga
    16621 acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg
    16681 ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg
    16741 ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAZBWR010000310        12672 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.084, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000310 DAZBWR010000000
VERSION     DAZBWR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.084"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..652)
                     /locus_tag="ACHGT5_003061"
     CDS             complement(<1..652)
                     /locus_tag="ACHGT5_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN7218945.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYL"
     gene            complement(857..2050)
                     /locus_tag="ACHGT5_003062"
     CDS             complement(857..2050)
                     /locus_tag="ACHGT5_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN7218946.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2129..2335)
                     /locus_tag="ACHGT5_003063"
     CDS             complement(2129..2335)
                     /locus_tag="ACHGT5_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN7218947.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(2328..3470)
                     /locus_tag="ACHGT5_003064"
     CDS             complement(2328..3470)
                     /locus_tag="ACHGT5_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN7218948.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3982..4617
                     /locus_tag="ACHGT5_003065"
     CDS             3982..4617
                     /locus_tag="ACHGT5_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN7218949.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4753..4845
                     /locus_tag="ACHGT5_003066"
                     /pseudo
     CDS             4753..4845
                     /locus_tag="ACHGT5_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4959..5594
                     /locus_tag="ACHGT5_003067"
     CDS             4959..5594
                     /locus_tag="ACHGT5_003067"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7218950.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            5701..6939
                     /gene="mef(H)"
                     /locus_tag="ACHGT5_003068"
                     /gene_synonym="mefH"
     CDS             5701..6939
                     /gene="mef(H)"
                     /locus_tag="ACHGT5_003068"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN7218951.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            7166..7585
                     /locus_tag="ACHGT5_003069"
     CDS             7166..7585
                     /locus_tag="ACHGT5_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN7218952.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            7590..7829
                     /locus_tag="ACHGT5_003070"
     CDS             7590..7829
                     /locus_tag="ACHGT5_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218953.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(8054..8977)
                     /locus_tag="ACHGT5_003071"
     CDS             complement(8054..8977)
                     /locus_tag="ACHGT5_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN7218954.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(9009..9773)
                     /gene="map"
                     /locus_tag="ACHGT5_003072"
     CDS             complement(9009..9773)
                     /gene="map"
                     /locus_tag="ACHGT5_003072"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN7218955.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            9931..10371
                     /locus_tag="ACHGT5_003073"
     CDS             9931..10371
                     /locus_tag="ACHGT5_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN7218956.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(10549..11118)
                     /locus_tag="ACHGT5_003074"
     CDS             complement(10549..11118)
                     /locus_tag="ACHGT5_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN7218957.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            11373..12605
                     /locus_tag="ACHGT5_003075"
     CDS             11373..12605
                     /locus_tag="ACHGT5_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN7218958.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         3919 a         2318 c         2371 g         4064 t
ORIGIN      
        1 ctaaataaat ctttgccgga taaaaattgt aaggaaaaaa accgataata cttgctacaa
       61 gacagaaaat aacaactgga atatacactg tagaagcatg gaggaaaaaa tatccaatga
      121 ttcctatcgt aattaaaccg atcaatgaaa tccctgccgc cagcccatcc aaaccgtcaa
      181 tcagattgac cgcattcgtg ataccgaaga tccaaaaaat cgttacggga aaactaagcc
      241 agcctagttg gatatatcca ataaaaggaa gcgtgaagga atcaatacgt atatctgcta
      301 caaaataaat aataagagaa gcaatcaaga tccctatact tttttgttta ggtgtcagct
      361 catatttatc atcgagcact cctgtgacta tgattactaa agaagcaatc aagatcggta
      421 caatatacga ttgagggata attttacgga ataagaataa agtcgatata acaaatgaaa
      481 taaagatagg cagtccgccc gctgaaggca ttggaacttt atttattctt ctttcatttg
      541 gtttgtcgac catacctgtt tgtactgaaa tgattttgaa tacaggtgtt aatatcagtg
      601 ataataaaat tgtcagaaaa aatcttataa ccatactaaa agaaacaagc atatgggcac
      661 ctactttaat taagagtcat aattattata aattatatat gtctttttga ctatcttttt
      721 atatcttctc aaatgtccgc aaagccttat tttatcggct ctacgggcat tttgcttttg
      781 tggtaaacct cgcatatcta ggtctatctt cttatatttt ccctgtcaat cgtggttaaa
      841 atcgtggtaa atacttctat gcgattagac gctgaacctc tgattttgcg gtatctatgg
      901 acgcatgagc gtaccagttc attgtgatac tgatgtttga atgccccatg atatactgta
      961 aatctttcgg gttcatgttc ttgcttgcca gccttgtgca gaatgtatgg cgtagcgtat
     1021 gcggtgtaat atgtggcaag ggattgtcct tgtggtgctt gttgtatttc tttaccatac
     1081 ggataaataa ggcgttataa tcaatggcaa cttttggatt acctttcgga ttgataaaca
     1141 caaaaccgct gtgtccgtct acctctatcg actttatctt tggtcggctg ttgataaccc
     1201 gttgaaatgc ctgtatcgtt tctttgctta atggcacttg ccttatcccg cttttcgtct
     1261 taggcgtttc aatataatag ccctgttcct tactctttag taactggtgg tcgataatca
     1321 caacttcatt cttgaaatca atgtcggcta ctgtcagtcc gcacagttcc gagatacgaa
     1381 gtcctgtctt taacagtatc agcacatcat cataatgctt gtgatacacg ttgtccgtct
     1441 taataaatga caataaggct tgctcctgtt cctctgtcaa tgcgactttc tctttggtat
     1501 cattttctat gacttcactt agcttgaaat caaaagggtt tttcctcaca taatcatctt
     1561 gtatggctat gtagaatgac gcttttaacg agcgtttatg gttgttaatg gtattgtagg
     1621 aaaagccttt gtctttcatg cgtaacgccc attctttagc gtcagagggt tttatcgtat
     1681 caatgctcct agcacctaac ttgtcctctt ttaataaccg catgagttgt tcccgttgtt
     1741 tctgtgtgct tttcttcaca tttgtcctct gtgcgttctg tttggcatag agttggcaaa
     1801 gcgtcatttt cttgcctgtg ctgtcgatac cgtcctcaat atcccgtctt atctgctgtt
     1861 ccagttctcg aagtgagggt ttttcccgtt ttccctttgg tgtcgggtct gtgggtgtca
     1921 atctccaagc atacacatat tttgtgtttc ccaatgcgtc tacatattta tataagtatt
     1981 ttccgtctgt tcgttggctc tctccagtat gcaggatacg acctttgtta tcccgtcttt
     2041 tttctttcat ggtgtctgct cctttccttg ttggaaagag ccttgatatg acttgtgttc
     2101 atcataacac atacaaggct catttgcatt agattgcgtc caatttatca ataacctttt
     2161 caaactgtcg gcgtttaatc tgtatgcggt tgccattcat aatgagccag ccagcgtcct
     2221 tgttttcctc tgccaatcgt cttaacttgt tttctccaat gcggaaatac tttgacgctt
     2281 cttcaatggt aagggtgtat ttttcccata cgggaatatc ctgtgtatta ctcataaaac
     2341 accccctcac tttcgattac gtctgatatg tccgtacaca tctgttccac aagcatttcc
     2401 tgtttttcag aaaggtgggt acattcatca atcattttca tgtagtttga acctgtcagc
     2461 ctgtcgattt cctctatcat cacgattgac ggcatgacct gtctttccag ccattgcaga
     2521 gtgcgttcca tattgacagg tacagggttc atttcaaaag gtaaactgtc atgggaaatg
     2581 aatatctccc acaatggata gtcaggaaat tccacaaaat cggtaatgag ataaaacacc
     2641 agcccatgag ggttatacgt caataacagt tcttcaactg cctgtaccgc ctttttgtca
     2701 cgcagacgaa tttcaaaacg gttgataata tcggtatgtt tctttttcgt tgcctgttcc
     2761 ttttgttttt catacaggca gaaatatttt gtgcttgcct ttgagccgat ataaagggta
     2821 cttgctaaat tacggttttt tccgcttaat tttccacctt gtacattttc atagttttta
     2881 cagcgacatt ccgcaccacc atttctgtat ttttcagata gcacaggaat atccagcaga
     2941 ccgcacatat cattgattgc caagtcaaaa cgtcggataa caccgccaca gtcaagacag
     3001 cggtttaaga atgaatacca gtccctgttc tgtgcctgta agacatattc catgttgcgt
     3061 gagcctgccc cttttaattc taaaaatacg cccatgtgtt catcatcaga agccatgacc
     3121 ttgatttccc cgtaagcata atgctcctta tagccataat acccatagtc ataatggata
     3181 aaataatctg ctttcatgcc aagcaccttt cggataactt ccaatgcgtc agtcgttgga
     3241 aaacggacag acagataatc aacgagcaga taaaaaggct tatcgcagat atagtaattc
     3301 agacaacgca ggataatgtt ctgtgtttcc ttgctggcgt tagcttttcc gttctcaaag
     3361 cggttgatac tatatcttga tacatgggta agacttgcaa gttctccttg tgaaagaccg
     3421 ctggcaagac gtttgttctt catttcaagt gcaaattcta aattgttcat tgtattcctc
     3481 caatcccaca atagccttga aatacctgta aaccaccttt caaaaagtgt gatatgtact
     3541 atcttcttaa aacctctcat tactactcac aggtttgggg gtgtatggtt tttgacttgt
     3601 cttttgtgaa atagtgcccc cctgttagat aacgggggca cagacaaggc tcgcaggctc
     3661 gccagccgac aaagcatttc ttccccttga cacaaggtgt catggcggta agcctgagag
     3721 gcttccgcca ccgtgtcaga aatactttgt cagcaagttc tgttttgcct tttctaacaa
     3781 gttccatttc aaccgatatt catatctacg gatttaatca gataggtatt tctttttgaa
     3841 tgtttttata tttcattcta ttgtgcttat tcgattacaa gaacatagta attcttatat
     3901 gcttatttgt caatatcttt ttttgccttt acaaatcata ttaagcatga tagacttata
     3961 atagcgaaga tataacgaga tatggagggt tatgcaatga aaataagcca tttaggaaat
     4021 aatatacaga ccataagaaa atttagggga atgaaacaac aggaacttgc ggacaaaatc
     4081 ggtatcaata tgcagagcct ttccaagatt gaaagagggt tgaactatcc tgcctatgaa
     4141 acgctggaaa agataatgga agtgctggac gttacgccca atgaattgtt gtcgggagaa
     4201 tggaagtatg tcaatcaatc tgaaaaggaa gtctgtcagt ttttaagagc cgaagaacgt
     4261 ctaaatgcag aactgaaaca tggacactat gataacttct ttgacagcga ggaagaatgg
     4321 ctggaatatg agttagaaaa gttacgggaa tatattacag actatatcaa cgggaaaagc
     4381 attgaagcgt ccgaccttta cccaatcaaa gaatttatcc agcatttgaa gtttcaaaaa
     4441 ttattagacc gctatgatga tttatacagt atggatatgt ttggggaaag catagaggga
     4501 cataagtata agacacctta tcaagtcgtg aaaatgataa acccgaactc aaaagaagat
     4561 atggaactgt tacgagaagt attaaggaat aacaattttg atgatgagga cgagtaactt
     4621 ttttcctctt gcgagaagat acagcaaaca aaaagcccga tcaagagtgc aaagcaccac
     4681 caatggtggc aaagctcttg acagggcttt ttcttttctg ttgtgggcaa tcaagaggaa
     4741 gaaagggata aaatctgatt tgtggtaaat caggcaaagg caacgcaggt atcacagtat
     4801 gaattgacac cgcataagga aagcaactgg aagattgtag aataaatgaa aaaggcttgc
     4861 attctgcatt tttataaaat tgcaagtctt tatattgaca agcataaaat actaagatat
     4921 actttaatca gaccaacggt ttgtattgga ggtaagttat ggcacgaaat aaatatccag
     4981 aagaaacagt aaaactcatt ttagatgttt ctacaaaaat gttttgtgaa aaaggatatg
     5041 ataacacatc attgcaagat attatcaatg aaacaaaact ttctaaaggt gctatttatc
     5101 atcatttcga ttcaaaggaa gatattttaa aagcaatttt tcaaagaatt ggaaatgaaa
     5161 atgcagtaat atttgctaaa attagagata acgatagttt aaacggttta gaaaagttaa
     5221 gagaaatatt taagacagcg gtattcaatt ctaaccaaag cgttttattg acagtttctc
     5281 ctcaactatt aaacaatcct cgttttcttg ctatgcaaat agaacaaatg tatgaaattg
     5341 tagtacctga atttgttgaa cccgttttag aacaaggtat caaggataag tctttggaga
     5401 ttgaaaattc acatgaaatt gcagaagtta tcatggtatt aagtaatgta tggctcaacc
     5461 cattagttga aatgacggat aaagagggta tggaaaaacg ttgcaataca ttcaatgccc
     5521 ttttaaatgg aataggcata gacacattat tagacgaaga aacaatttct gcatttatac
     5581 agttttgtaa ataactgtat ttgttatccc tatacgatag actgctctat aaaagagcag
     5641 tttatttaaa ccattatata aaccgacggt ctgtttgatt aaataaaaag gagagatttt
     5701 atgcaagcag aaaaaaaact ttttaatcgt gattttatac ttgttgttat aggacagata
     5761 atttctttat ttggaaacgc tatcctacga tttgcattac cgtactattt gttaaaagaa
     5821 actggttcat cagctttgtt tggtattgct acagcctgct cttttttacc tatggttatt
     5881 ttgtcgctgg taggtggtat tattgctgac aggacaaata aaagaaacat tatggtcata
     5941 ctagattttg taacagcaat cataatttta gtattttatt tgtcattggg aaaaattcct
     6001 atcatacctt tgtttattgt tgctttgatg ttactttatg gtatttctgg tgcatatcaa
     6061 ccagcggtac aagcaagtat tcctctattg gtatcatcag ataaacttac cgtcggtaat
     6121 gctataataa atcaagtaaa tactttagca aactttatcg gaccagctat aagcggagta
     6181 gtttttcaat tttatggtat caatcccatt ttaattgtta gtattatatg ttttactttt
     6241 tcagccatta tggaaatttt tatccatatt ccacaccaaa aaagacctcg tgaagtaggt
     6301 gtaattgcag ttgctaaaaa cgatttaaaa gatagttata cctttttgaa aaaggagaaa
     6361 cccattttta tatccgtaat ctttttggtt tgcattttca atttggtttt aagtgctgtt
     6421 ttaattgttg gtctgcctat cttaatttct gaagttttaa aaatgccaga tacactatat
     6481 ggtttttcac aatcagcatt atcattaggt ggtttatgtg gtggtatttt aacagctctt
     6541 gttgcttcaa agttaaaact aaaaaacctt tataccttgt tacttatttg ttctgtttct
     6601 gttttactta tgggaatttc gctattttta gaattgcctg caatagtttc ctactgggta
     6661 gttacagtga tgtgctttgt ggctatgggt gtatctacta tgtttgttgt tcaaatttac
     6721 actcttgttc agacacaaac accaccgcaa ttagtaggaa agataatggc tacgctggta
     6781 gcagttgcta tgtgcggtca acctattgga caaatggttt acggtatttt attcgatgta
     6841 tttatacaag aaacatgggt tgttatgttt gtagcttcta ttgcttcaat catgatttca
     6901 atatattcaa aaagaatttt tcaaaagtta gagatataat acaaggggtt aaatagtatg
     6961 ttaggctaaa aacaatatat aaaagaaaat ttgaaatctg ctaaaaggat agtgttataa
     7021 aagaaagagt aatattgcag tatttaaggc tcgcacaaat atatgttttg tgtgggcttt
     7081 tttcttacca tttttctgta tttctaaagg gtataaacgg tattccgttt atatatttca
     7141 gcattagcag aaagggggtg agattatgag gacatcttct ttcgagcata tcgtcagatt
     7201 acagttcaat tctttaatgc tgattgtcat taagaacaaa gtgaaaagtc gaaacagaca
     7261 gcttgcaaga cattctaaac gtgaagtatt gttttgtgaa atagccgaaa cgaaacaagt
     7321 tgaacgtgga acgattgata cctgttttta tgattgtgta tcatttaaag tcttgcactt
     7381 tacaatttat gtatctgatg aaacacttgg tacagtttta catagactgt cagaaaaaca
     7441 gcgtagtgcg attttattac gttattttca aggaatgaac gacagagaaa tttcagaact
     7501 gtaccatgta tcacgttctg ctatttgcag tagaagaact agaggactaa ggaagttaaa
     7561 aatgcttttg agcgaaagga agtaaagaaa tgagtaaaga ttatggctat ccctcttttg
     7621 aattgatttg caaagcgtca agcggagatg aaatggcagt taaagagatt ttgaagtttt
     7681 atgacggtta tatttctaaa ttatgcttgc gtccttttta ccactctgaa agtggtaaaa
     7741 tcattatgca ggttgatgaa gaactaaaag gagaaattca tacagacatg atgaaagcaa
     7801 tattgaaatt tgaaatcgga gtgaagtaat caactgtata aaaaaatcgg gagatacaca
     7861 gtttcgtgct atctccctta gtagttttac ggtcatatca aggctcattc aatcgtggta
     7921 aattcgtggt aagatgagtg tttttctata cttcaaaatg cttgaatgta tagtgtttat
     7981 gcggataatt gaaaattaga tataaaattt caaataaatt actgatataa atttacaata
     8041 agaactcttg tttctacttt tttttatgag aaaatttagt tttcaattta ttgaaccatt
     8101 ttcggcttgg atctcgacgt tttttgattt gatgacccgt cacgtattct tgaacaactg
     8161 cattgatgat tccgcccaaa atgatgatcg tagcagcaaa attaagccaa agcatgagta
     8221 caataaaact tcctatgatc tgataaccgc tgatcctcgt tgcaaaatac ctagcataga
     8281 tactaaacac ttgtgataac agcatccagc cgacagtagc gaagatcgtg cccggaatga
     8341 tcgtcttcaa cttcaactga gcattaggga caattaaata aatcaagaac atgatgacaa
     8401 ataaagctac caatgtgatt ggccacttca aagtttggaa ctgatcgata aagttcacgg
     8461 gtatttgtac gattggctga atagcatcca aaatcagctg tcccaaccca agaacgatag
     8521 ttaccccact aatagcaatc atgaacaaga caatgacaat tagtgagaag aaacggacga
     8581 tcaaaaaatt ttttctattt tctacgccat acgctttatt catcgcaatt tgcaaagcat
     8641 tgatactttg acttgcagac cataaagccg ctaatgcaga aacggataac aagcctcctg
     8701 aactttgtgt caaaaggtct ttgattgcag gaccgataaa ctggtaaacg gtctcaggaa
     8761 tcacttctcg taaataggtc agtacagtct cttgatcgat gtggagaaac ggtaaaatat
     8821 taccaaaagc aataatcaat ggaaataaag acaagagtaa ataataagca acgacgacag
     8881 aggtcgtact catttctgaa tcagtgatgc gcttttgagt cgtctcaata aagcgcatca
     8941 gattttcatt ttgttttatt ttgttccaaa ctttcatgta cctctcccgt acctattgac
     9001 tggatagatc aataagtgcc ttcttctcct tgtgatgtca aaattcttgg accatcttta
     9061 gtaatagcca atgtatgttc atattggcaa gaaagtccgc cgtctcttgt atacgcagtc
     9121 cagccgttag gatccatttt cattttccaa gttccggtat taaccatagg ttcgatcgtg
     9181 ataaccatgc cttctttcaa ccgcaaccct tttccagctt ctccgtaatg aggaactgct
     9241 ggttcttcat gaatcgtagg accaacgcca tgaccaataa aatcacgaac gatcgctaga
     9301 ttttcgcctt ctacataagt ttggatagca tgaccgatgt caccaatccg attccctact
     9361 tgtgcttgct caataccgat gtacaaagct ttcttcgtta cttccatcaa tcggtcgatc
     9421 tcaggtgtag attctccaac tacataggcc caacaagaat cagaaatgcc gcctttcaaa
     9481 tcgatacaca tatcgacttt taccaaatca ccgtttttca aaggcttttt acgagggaat
     9541 ccatgacaaa tttcatcatt tatactgata caagtcgcat attcataccc ttcaaagcca
     9601 atttgcgcag caattccgcc atgactctcg atatagtttc tgacaaatac ctcaatatcc
     9661 caacttgtga ttcctggttt tataaaatca cgcagatttt tatgtacatc tgctaataaa
     9721 gctccagatt ctgccatcat ttcaatttca cgtgctgatt ttaaagtaat catattttcg
     9781 tctccttttt acaaatccct ctcattttaa catagcggca tacatttagc caaatgacat
     9841 tccccaggtg tctctaggtt ttattttctt ttcaatgatg ttttggtata attcataatt
     9901 gttaaataat tataaacgag gaggcacatc atggctttag caaaaatcgt atatgctagc
     9961 atgacaggaa acactgaaga aatcgccgat attgttgcag aagcatttga aaatctggat
    10021 atcgaagtcg aaatcaacga atgtacccaa gtagatgcag atgaattcga ggaggcagat
    10081 atttgcgtag tagctactta tacatacgat gatggcgatc tgcctgatga gatcgtagac
    10141 ttttatgaag acttgcaaga attggatcta ttgggaaaaa tttacggtgt ctgcggatct
    10201 ggtgatactt tttacgatga attctgcaaa tcggtggatg atttcgatgc tgcttttgca
    10261 aaaacaggcg catcaaaagg agccgaaaat gttaaagtgg atctgaatgc tgaagaagaa
    10321 gatatcgaaa atttagaagc tttcgctaaa gaactagtcg caaaaattta atatacttgc
    10381 aataaaagag ccatccagat ttattcaatc tgtatggctc ttttattcat tgcattcatg
    10441 gaagagttct tcgggagtta aaaatgcata cgctgtaaga aaaacgaact tttccttatt
    10501 ttgacacatc tcttgttacc tactgatttg tggaactttt gagtatattc aatcgatttc
    10561 gattggttct ttgaatttca agatagcatc tatactgtct tggatcatct tttcaatctg
    10621 ttcatcagtt ggatggatga tgcttgaaga catcaaagct gcgatcccag tagctatgat
    10681 ccatgtccca ttatacaaag attcgatctg ctcttctgaa agattcacat aagctggatc
    10741 gcgtttcact gtattcacaa agtaagtata agagaaattc tgcatctctt ttcctccacc
    10801 atattcttct aagtacagtg aacgatacaa cttcttctct cttcttgcaa aatggatata
    10861 attaaaagca agatcgataa tcgtattccc tgtatgctca acagtgaaca cttcatattg
    10921 taaatgatca tagatctgct caaaaagagc ttgtctcaag tcatccatat ttttaaactc
    10981 aagataaatc ggttgcgtgg aacaattcat tttgttcgcg atgtttctcg cagtaaatcg
    11041 tgtaaatcca tcggtggcag ctacttcata agcagctgcc aaaatctgtt ctctagtaat
    11101 cgtctttttt cttgccatat tttttaatct ccttaatttt tttataacgc ttccaccatt
    11161 gattaatttc attgtaacat gttttttttg atatgcaaaa ttagtttttg attttcttca
    11221 caagctgaaa tagcaagtag taacatatat catgaaactt aaccttaaaa aaataacctc
    11281 ctttttttat ttaaagcctc attgtttgaa tcataattaa atggtatcat gctaatagtt
    11341 accagaaaaa atggtgagga ggaattttta ttatgttatc tgattttaat gaacttctaa
    11401 aaaaatatgc acgcttaatt gcagaaactg gcgtagccac agaaaaagga cacacagttg
    11461 ttttacagat cagcgtggaa caggcacccc ttgctcgatt gatcacacaa gaagcttata
    11521 aactaggagc cgctgaggtt atcgttcaat ggactgacga ccagatccaa agagaatttc
    11581 ttttacatgc tgcaacggat cgtattgaga atgtaccaca atctaaaatc gatcaagcgg
    11641 atgaatggct tgaaaaagga gcaagcagga tcagcgttgt ttctgccgat cccgatgcat
    11701 ttgctggtgt agatagccat cgagtagcgt cttatcaatc agcgaatggc aaagcactaa
    11761 tgaatttacg caaggcaact caagcaaata aagtcagttg gactgtcgtg gctgcggctg
    11821 gtaaacaatg ggcagcaaaa gtcttcccag aactgccaga agaaaaacaa gttgaggcat
    11881 tatgggatca gattttcaaa acgactcgtg tctatgaaga agaccctgtc ttagcatgga
    11941 agaagcatga tgaaaaatta gcaaaaaaag cagaagaatt gaatcgagaa caattttctg
    12001 cccttcacta tactgctccc ggtacagata tcattatcgg attgcctaaa aaccatcttt
    12061 gggaaggcgc tggaagctat aatgctcgtg gagaaaaatt catggcaaat atgccgacgg
    12121 aagaagtgtt cacagcacct gacagtcatc gagtagatgg ttacatttca agtacaaagc
    12181 cactgagtta tgctggcacc atcatctcgg gtatgaaatt tacttttaaa gatggaaaag
    12241 ttgttgattt ttctgccgaa caaggagaag atgtcttagc aaaactgctt gatacagatg
    12301 aaggtgcgcg tcgtttagga gaagtcgcac ttgtacctga cccttcacct atttctcaat
    12361 ctggcatcat ctttttcaat acattatttg atgaaaacgc atcgaatcac ttagcactag
    12421 gttctgctta tgccttcagt gtgaaaggcg gtacagaaat gtccgatgaa gaattagcag
    12481 aagctggatt gaatagaagt caaacccacg tagatttcat ggtaggatct gacaagatgg
    12541 atatcgacgg aatccgtgaa gatggctcta cagtaccgat tttccgaaat ggcgattggg
    12601 cttgagtata ctttcgcaca catgcaaaaa ataatggctc tttgtcaata aggactgatg
    12661 agttgtgcaa aa
//
LOCUS       DAZBWR010000311        10087 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.105, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000311 DAZBWR010000000
VERSION     DAZBWR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.105"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACHGT5_003076"
     CDS             353..523
                     /locus_tag="ACHGT5_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218959.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACHGT5_003077"
     CDS             537..1139
                     /locus_tag="ACHGT5_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7218960.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACHGT5_003078"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACHGT5_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACHGT5_003079"
     CDS             2143..2640
                     /locus_tag="ACHGT5_003079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFN7218961.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACHGT5_003080"
     CDS             2641..3057
                     /locus_tag="ACHGT5_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFN7218962.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACHGT5_003081"
     CDS             3077..4621
                     /locus_tag="ACHGT5_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN7218963.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACHGT5_003082"
     CDS             4764..4988
                     /locus_tag="ACHGT5_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN7218964.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACHGT5_003083"
     CDS             5003..5872
                     /locus_tag="ACHGT5_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFN7218965.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACHGT5_003084"
     CDS             5853..6587
                     /locus_tag="ACHGT5_003084"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN7218966.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACHGT5_003085"
     CDS             6620..7528
                     /locus_tag="ACHGT5_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFN7218967.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACHGT5_003086"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACHGT5_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFN7218968.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACHGT5_003087"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACHGT5_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFN7218969.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACHGT5_003088"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACHGT5_003088"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACHGT5_003089"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACHGT5_003089"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAZBWR010000312         9360 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.113, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000312 DAZBWR010000000
VERSION     DAZBWR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.113"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..146
                     /locus_tag="ACHGT5_003090"
     CDS             <1..146
                     /locus_tag="ACHGT5_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN7218970.1"
                     /translation="FLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSV
                     ENK"
     gene            complement(296..1237)
                     /locus_tag="ACHGT5_003091"
     CDS             complement(296..1237)
                     /locus_tag="ACHGT5_003091"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN7218971.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1363..2484
                     /locus_tag="ACHGT5_003092"
     CDS             1363..2484
                     /locus_tag="ACHGT5_003092"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN7218972.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2742..4244
                     /gene="eat(A)"
                     /locus_tag="ACHGT5_003093"
     CDS             2742..4244
                     /gene="eat(A)"
                     /locus_tag="ACHGT5_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN7218973.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4311..4838
                     /locus_tag="ACHGT5_003094"
     CDS             4311..4838
                     /locus_tag="ACHGT5_003094"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN7218974.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5049..6110
                     /locus_tag="ACHGT5_003095"
     CDS             5049..6110
                     /locus_tag="ACHGT5_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN7218975.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6113..7009
                     /gene="murQ"
                     /locus_tag="ACHGT5_003096"
     CDS             6113..7009
                     /gene="murQ"
                     /locus_tag="ACHGT5_003096"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN7218976.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7023..9005
                     /locus_tag="ACHGT5_003097"
     CDS             7023..9005
                     /locus_tag="ACHGT5_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN7218977.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9070..>9360)
                     /locus_tag="ACHGT5_003098"
     CDS             complement(9070..>9360)
                     /locus_tag="ACHGT5_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN7218978.1"
                     /translation="PFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGV
                     VGSGNKNFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3063 a         1675 c         2044 g         2578 t
ORIGIN      
        1 aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt tatgatgcag
       61 aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg
      121 acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa
      181 caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg
      241 ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt
      301 ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg
      361 tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag
      421 tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt
      481 caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt
      541 cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt
      601 tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc
      661 catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta
      721 aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca
      781 agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac
      841 cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta
      901 catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg
      961 attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt
     1021 ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca
     1081 cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag
     1141 gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc
     1201 tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat
     1261 gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa
     1321 tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca
     1381 attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc
     1441 tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact
     1501 gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc
     1561 ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca
     1621 gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact
     1681 atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa
     1741 gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta
     1801 ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact
     1861 aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct
     1921 gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa
     1981 aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag
     2041 caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc
     2101 ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc
     2161 agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa
     2221 gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca
     2281 aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga
     2341 ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa
     2401 gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga
     2461 tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc
     2521 aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg
     2581 cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg
     2641 gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt
     2701 tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa
     2761 aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa
     2821 attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt
     2881 tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat
     2941 ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg
     3001 atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc
     3061 ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac
     3121 tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt
     3181 tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca
     3241 tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg
     3301 aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac
     3361 ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa
     3421 agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga
     3481 aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg
     3541 atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca
     3601 gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc
     3661 aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc
     3721 tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc
     3781 ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg
     3841 ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg
     3901 aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat
     3961 ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc
     4021 agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg
     4081 aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag
     4141 aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc
     4201 tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag
     4261 aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg
     4321 taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg
     4381 tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt
     4441 tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac
     4501 aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg
     4561 attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag
     4621 cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc
     4681 ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg
     4741 ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat
     4801 tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa
     4861 taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct
     4921 tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga
     4981 aattttgttt ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga
     5041 gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac
     5101 agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac
     5161 cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa
     5221 agaataaact agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt
     5281 cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc
     5341 atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta
     5401 gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga
     5461 ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc
     5521 agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag
     5581 atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga
     5641 tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg
     5701 gagatgcagg tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga
     5761 cagtcttgct ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc
     5821 atacgaatcg gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag
     5881 agattccgcc tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag
     5941 ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag
     6001 cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc
     6061 aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa
     6121 aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga
     6181 gatgagtatt tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc
     6241 ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa
     6301 aaaaggtgga cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga
     6361 tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc
     6421 tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg
     6481 gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc
     6541 tagcggtcgt acaccatatg tgatcggtgg attagattac gcaaaatcga tcggtgctgt
     6601 cacaggaacg atttcttgca ataaacaagc cgaaatcagt aaacacgcgg atttcccaat
     6661 cgaagtcgat gcaggtccag aatttttgac aggctctact cgattgaagt caggaacagc
     6721 acaaaaactt atcttgaata tgatttctac tatcagtatg attggtatag gtaaagtatt
     6781 caataacttg atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg
     6841 aatcatcatg caagcgacag aagctgatta cgaaacagct gaaaagtact ttgaagaagc
     6901 agatcaaaat gttaagctgg ctatcgtcat gattttgaca aatagtgata aagaggaagc
     6961 tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg accttatgag ggggaaaaga
     7021 ccatggcaaa aaacgaactg actttggaag aactggcaaa acaaatttat aaaggggcag
     7081 gcggcatggg caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt atgaagatca
     7141 tcaatgatga tcttgtaaat gtctctgaat taaaagcgat tccaggagtg cttggtgtag
     7201 tagaagatga acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc
     7261 aaatggttga tttggcaggc gtgaaattag gagaaaaact ttcttctgta agtacagcta
     7321 cagcaaattc atcagcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag
     7381 cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa gacatttcta
     7441 atatctttgt gccaatgatt cctgcatttg tcggtactgg gatcgtggca gggattgccg
     7501 cagttttagc aaacttagtc acagccggaa cacttgatgc agcaacatgg caacaatata
     7561 tcgatgtgat gaacatcttg aaaaacggga tgtttagcta cctagttatc tatacaggta
     7621 tcaatgctgc acaagttttt ggtgcaacac caactcttgg tggagttatc ggggccgttg
     7681 tgatgctgac agggatgaat ccggaagcac cactgaaaaa cttatttact ggtgaagctc
     7741 ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg
     7801 ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt acaccaactc
     7861 tttcattgat tgccatcggt ctgattgaaa ttttcttcat catgccttta gcaggtttca
     7921 tttctgatgg catggtcggt gggatcaact gggtacttgg cgttggtgga gcatttgccg
     7981 gcttcgttct aggcacatta ttcttgccga tggttatgtt cggactgcat caaattttga
     8041 ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt ccaattcttg
     8101 cgatggctgg tgcaggacaa gtgggtgcag cattggcatt atggattcgc tgcaaaaaag
     8161 ataaagaatt aacagaaatg atcaaaggag ctttaccagt cgggatccta ggaatcggcg
     8221 aaccattgat ttacggagta acattacctc taggacgccc atttattact gcttgtatcg
     8281 gtggaggtat cggcggggca gtgatcggaa tgatcggaaa tgtcggggct atcgctattg
     8341 gaccttcagg agccgctttg atcccattga tctctgacgg taaatggtta ggctacatcc
     8401 ttggattatt ggcagcttat gctggtggct tcgttgctac attcttcttc ggtattccta
     8461 aagaccgttt agaaaaagaa gaattagcga ctgaaacagc cacagtaaat gaagtgcctg
     8521 aaaaagtagt acctcaaact tcagctgaaa gtgtgactat cctctcatca gttgctgacg
     8581 gaacagttgt ttctttagat aaagcaagtg atccagtttt tgcacaaaag atgatgggtg
     8641 aaggctactt tgtagaacca gaaaacggac atatctattc tccagttact ggggtagtca
     8701 gctctgtctt cccaacgaaa cacgctattg ggatcacaac gccaaatggt ttagaaatct
     8761 tgcttcatat gggaatcaac acagttgact taggcggtac gccatttgat gtgaaagtaa
     8821 cagaagggca gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcgatca
     8881 aagcagcagg caaagaaaca tccatgatcg tcttggtaac caatatggat cgtgtaaaaa
     8941 actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag
     9001 cttaatatga tagaaacaat agaaacgaaa gaagaaactg aaatctattt ttcagcttct
     9061 tcttttttat tatttattgt tttctgctaa aatctgataa attcgtgtca catcagatgg
     9121 tgttccccgt aattcataat ctgccaagaa aggaaagcca aatgtttgtg catattgttt
     9181 ggctgttaga caatactgat tattgaagtt tttatttccg ctaccgacta caccaagaca
     9241 aagtttatga ttgtcttcat gttccagata ttcacgcatc gtttcggtca agatttctgt
     9301 atcaccgtta tctaaaccat ttccgccgtc taaataagta ggaacgaaag taaaaaatgg
//
LOCUS       DAZBWR010000313         2772 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.197, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000313 DAZBWR010000000
VERSION     DAZBWR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.197"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACHGT5_003099"
     CDS             111..194
                     /locus_tag="ACHGT5_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN7218979.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHGT5_003100"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHGT5_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN7218980.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACHGT5_003101"
     CDS             complement(1319..2182)
                     /locus_tag="ACHGT5_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN7218981.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACHGT5_003102"
     CDS             complement(2184..2456)
                     /locus_tag="ACHGT5_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN7218982.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACHGT5_003103"
     CDS             complement(2473..2682)
                     /locus_tag="ACHGT5_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN7218983.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAZBWR010000314         2586 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.201, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000314 DAZBWR010000000
VERSION     DAZBWR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.201"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..630
                     /gene="dfrG"
                     /locus_tag="ACHGT5_003104"
     CDS             133..630
                     /gene="dfrG"
                     /locus_tag="ACHGT5_003104"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN7218984.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(702..>2586)
                     /locus_tag="ACHGT5_003105"
     CDS             complement(702..>2586)
                     /locus_tag="ACHGT5_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN7218985.1"
                     /translation="EELKDRAKQYDLLTPKRFATRYNKMLFLPTSFKWNGSEYPIQYN
                     YCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKDKGHKGMYCNDNPIGRGVSQNCTV
                     TPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGCSEEESTPFNEPKQFYKRGKSRGK
                     SQRYQCKACKKFTNVLPKREETTTYHQQKNTILPMFAKMVVGRVSVSRTCDILGIGVG
                     TYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWLNTDKMHYYLNNVRKKGQGSKKYT
                     GFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISMDELNEDTRKFKEDHLNTFSRKND
                     RLDWSYYPQEPSANDSENRNAYLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMV
                     NADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAE
                     LLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLAT
                     KDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGD
                     KKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDII
                     YFK"
BASE COUNT          796 a          433 c          460 g          897 t
ORIGIN      
        1 ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac taacgggcag
       61 gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt agttaaaaag
      121 gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag agtgattggc
      181 aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa aaatactaca
      241 aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag agccttacct
      301 gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg ttgtgaaatt
      361 gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat ttttattttc
      421 ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta catcacaaaa
      481 atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga ggaatggaat
      541 gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta ctattttcat
      601 gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa gaacagtaaa
      661 gataaagcac aagagcttgg gattactcca agctcttttt gtcatttgaa ataaattata
      721 tctttccaat caaattgttt atctgtaatc cctaatcttt gagcaggtgt caaaaccttt
      781 ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat tgtaatcgca
      841 aattgagcat acttcggatt gaagttagcg taaatatagc tcttcttatc accacgagct
      901 gttgttaatg gtctttcaag agatgatata taacgtctta tctgttgaat aaagctattg
      961 gtcgaatggt cattaacatt taatagcatc tttgcgattt cgtttggttc cagagcagat
     1021 aaatccgttc tacaatcaac tgaataaaat cctttatcct tagtggctaa tggatgtttg
     1081 atagggttat cggcatattt gcgatatctt tctccatctt tactaatagc ctcttcgtgg
     1141 aattgatgac ttgttagtaa ttcagttaga taatgagatg ccaaagtccg tagaaacttt
     1201 gttttgaatc ctctgatatc tccccaatcg agcagttctg cttttgcttg tccgaattct
     1261 tttaagcatt gttttctgct tttcgtcttg ttaacttggc aaataaaatg atgtgcatcg
     1321 ctcaacctta attctttcgt gaatactcta taaaaggcgt ttctaataga gaagtcatca
     1381 tcactaatca ttcgccattc gtcggcatta accatttgtt taattaacca atagtgagca
     1441 gttgtagtat aaggagcatc aacatttaaa ccatcgacaa atctactcct attagtaatt
     1501 ttccctaatt cgtgaagata agcattccta ttttcgctat cgttagcgga tggttcttgt
     1561 ggataataag accaatctaa cctatcattt ttacgactaa aagtattgag atggtcttct
     1621 ttgaattttc ttgtatcttc atttaattca tccattgaaa tattccaatc ataggcaaca
     1681 tcagaacgga acacatatct tgataagacc tcagcagata caactatata cgtttgcata
     1741 ttaaggtctt caaaccccgt atatttcttt gaaccttgtc ctttcttacg gacgttattt
     1801 aggtagtagt gcatcttgtc cgtattaagc cacatctcgt taaatttctt tgtttgaagg
     1861 ggttgtgtct catatcgttc taaaaactcc aaacaacgcc tatacagcca ctccagctta
     1921 tgatagtagg ttccaactcc aattcctaaa atatcacaag tacgactgac tgaaaccctt
     1981 ccaacaacca tcttagcgaa cattggaaga atggtgttct tttgttggtg ataagtagtg
     2041 gtttcttctc tttttggtag aacattagtg aacttcttac acgccttaca ttgatagcgt
     2101 tgagatttgc ccctactttt cccccttttg taaaattgct tcggctcatt gaatggggtt
     2161 gattcttctt ccgagcagcc ttctttatga aattgataat ctggctcaac atcttggatg
     2221 ctattgattt ctataaggcg tttaatttct tcaacaacag accaattaga taaaggggtt
     2281 actgtacagt tttgcgatac acctctgcca ataggattgt cattgcagta cataccttta
     2341 tgacctttat ctttggaact accagtcatt ttatatcgac ttggtttccc ttttacatct
     2401 ttgaatttgt gctgttcttt cccaaaatta caacaaaacg gattgatgca gtaattatat
     2461 tgaatgggat attcagaacc gttccattta aaagacgttg gcaagaacaa cattttgtta
     2521 taccttgttg caaatcgttt tggagttaat aaatcatatt gtttagccct atcttttaat
     2581 tcttcg
//
LOCUS       DAZBWR010000315         2056 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.219, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000315 DAZBWR010000000
VERSION     DAZBWR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.219"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACHGT5_003106"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACHGT5_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACHGT5_003107"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACHGT5_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACHGT5_003108"
     CDS             complement(1055..1345)
                     /locus_tag="ACHGT5_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218986.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1747..1983
                     /locus_tag="ACHGT5_003109"
     CDS             1747..1983
                     /locus_tag="ACHGT5_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN7218987.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          693 a          355 c          325 g          683 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt
     1681 gtaggaatgc aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat
     1741 tttcctatgc aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata
     1801 tcagagcaag taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc
     1861 gaagcagtgg atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt
     1921 gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt
     1981 tgatagacaa tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt
     2041 aaaattccga tttcaa
//
LOCUS       DAZBWR010000316          761 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5121
            SAMEA115707508-rid22987113.guided.283, whole genome shotgun
            sequence.
ACCESSION   DAZBWR010000316 DAZBWR010000000
VERSION     DAZBWR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707508
            Sequence Read Archive: ERR13197237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002338199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002338199.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 13:41:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,964
            CDSs (with protein)               :: 2,964
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 49 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707508-rid22987113.guided.283"
                     /isolate="VRE5121"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..696
                     /gene="aac(6')"
                     /locus_tag="ACHGT5_003110"
     CDS             148..696
                     /gene="aac(6')"
                     /locus_tag="ACHGT5_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN7218988.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          277 a          111 c          169 g          204 t
ORIGIN      
        1 aagtgtttct gtataatttt ttattttata agtaagataa ataatgaaag aatagagata
       61 ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag
      121 aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca
      181 gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga atatggagac
      241 agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta
      301 gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg
      361 gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta
      421 gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg
      481 gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat
      541 aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt
      601 tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg
      661 gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta
      721 atcagcttca agaaataaga aggaatttac gaaaattact t
//
